Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0084500 | Msa0667850 | 0.807043 | 6.128372e-50 | -8.615850e-47 |
| Msa0641360 | Msa0667850 | 0.806407 | 8.350259e-50 | -8.615850e-47 |
| Msa0667810 | Msa0667850 | 0.810340 | 1.209891e-50 | -8.615850e-47 |
| Msa0667850 | Msa0667860 | 0.811724 | 6.068559e-51 | -8.615850e-47 |
| Msa0667850 | Msa0669840 | 0.800933 | 1.142381e-48 | -8.615850e-47 |
| Msa0667850 | Msa0682530 | 0.809828 | 1.560487e-50 | -8.615850e-47 |
| Msa0667850 | Msa0742180 | 0.819458 | 1.149393e-52 | -8.615850e-47 |
| Msa0667850 | Msa0764820 | 0.808931 | 2.430475e-50 | -8.615850e-47 |
| Msa0667850 | Msa0786040 | 0.809622 | 1.727203e-50 | -8.615850e-47 |
| Msa0667850 | Msa0823400 | 0.818893 | 1.545587e-52 | -8.615850e-47 |
| Msa0667850 | Msa0862100 | 0.807538 | 4.813659e-50 | -8.615850e-47 |
| Msa0667850 | Msa0862310 | 0.812721 | 3.676059e-51 | -8.615850e-47 |
| Msa0667850 | Msa0885060 | 0.854068 | 1.574333e-61 | -8.615850e-47 |
| Msa0667850 | Msa0929870 | 0.807459 | 5.004123e-50 | -8.615850e-47 |
| Msa0667850 | Msa0999340 | 0.833757 | 4.439057e-56 | -8.615850e-47 |
| Msa0667850 | Msa0999350 | 0.839220 | 1.805155e-57 | -8.615850e-47 |
| Msa0667850 | Msa0999360 | 0.817969 | 2.502278e-52 | -8.615850e-47 |
| Msa0667850 | Msa0999390 | 0.817327 | 3.492483e-52 | -8.615850e-47 |
| Msa0667850 | Msa0999400 | 0.832015 | 1.203026e-55 | -8.615850e-47 |
| Msa0667850 | Msa0999410 | 0.812079 | 5.077047e-51 | -8.615850e-47 |
| Msa0667850 | Msa1007860 | 0.814608 | 1.412912e-51 | -8.615850e-47 |
| Msa0667850 | Msa1033560 | 0.820911 | 5.340440e-53 | -8.615850e-47 |
| Msa0667850 | Msa1034680 | 0.800521 | 1.386445e-48 | -8.615850e-47 |
| Msa0667850 | Msa1073340 | 0.855722 | 5.213166e-62 | -8.615850e-47 |
| Msa0667850 | Msa1113850 | 0.827868 | 1.233394e-54 | -8.615850e-47 |
| Msa0667850 | Msa1145020 | 0.806100 | 9.690403e-50 | -8.615850e-47 |
| Msa0667850 | Msa1160400 | 0.811188 | 7.930710e-51 | -8.615850e-47 |
| Msa0667850 | Msa1201160 | 0.813788 | 2.143145e-51 | -8.615850e-47 |
| Msa0667850 | Msa1228190 | 0.825756 | 3.940927e-54 | -8.615850e-47 |
| Msa0667850 | Msa1271260 | 0.833973 | 3.919975e-56 | -8.615850e-47 |
| Msa0667850 | Msa1345420 | 0.803394 | 3.560173e-49 | -8.615850e-47 |
| Msa0667850 | Msa1378770 | 0.815397 | 9.440299e-52 | -8.615850e-47 |
| Msa0667850 | Msa1420060 | 0.813607 | 2.349499e-51 | -8.615850e-47 |
| Msa0667850 | Msa1421550 | 0.803716 | 3.052745e-49 | -8.615850e-47 |
| Msa0667850 | Msa1424690 | 0.812393 | 4.336038e-51 | -8.615850e-47 |
| Msa0667850 | Msa1435480 | 0.831435 | 1.672113e-55 | -8.615850e-47 |
| Msa0667850 | Msa1458910 | 0.842393 | 2.657949e-58 | -8.615850e-47 |
| Msa0130570 | Msa0667850 | 0.811830 | 5.752550e-51 | -8.615850e-47 |
| Msa0191040 | Msa0667850 | 0.805506 | 1.291570e-49 | -8.615850e-47 |
| Msa0194380 | Msa0667850 | 0.822229 | 2.649136e-53 | -8.615850e-47 |
| Msa0203030 | Msa0667850 | 0.816019 | 6.860389e-52 | -8.615850e-47 |
| Msa0206800 | Msa0667850 | 0.800385 | 1.477615e-48 | -8.615850e-47 |
| Msa0233340 | Msa0667850 | 0.804555 | 2.042425e-49 | -8.615850e-47 |
| Msa0236110 | Msa0667850 | 0.821572 | 3.759321e-53 | -8.615850e-47 |
| Msa0373960 | Msa0667850 | 0.856703 | 2.688815e-62 | -8.615850e-47 |
| Msa0382010 | Msa0667850 | 0.832338 | 1.000745e-55 | -8.615850e-47 |
| Msa0423100 | Msa0667850 | 0.847097 | 1.434740e-59 | -8.615850e-47 |
| Msa0436500 | Msa0667850 | 0.825218 | 5.284904e-54 | -8.615850e-47 |
| Msa0469260 | Msa0667850 | 0.837166 | 6.102772e-57 | -8.615850e-47 |
| Msa0476590 | Msa0667850 | 0.819185 | 1.326345e-52 | -8.615850e-47 |
| Msa0273990 | Msa0667850 | 0.809608 | 1.739757e-50 | -8.615850e-47 |
| Msa0277290 | Msa0667850 | 0.807829 | 4.175753e-50 | -8.615850e-47 |
| Msa0288190 | Msa0667850 | 0.808036 | 3.772314e-50 | -8.615850e-47 |
| Msa0298240 | Msa0667850 | 0.803906 | 2.787094e-49 | -8.615850e-47 |
| Msa0311690 | Msa0667850 | 0.813777 | 2.155684e-51 | -8.615850e-47 |
| Msa0314630 | Msa0667850 | 0.819001 | 1.460723e-52 | -8.615850e-47 |
| Msa0520510 | Msa0667850 | 0.860854 | 1.544869e-63 | -8.615850e-47 |
| Msa0533080 | Msa0667850 | 0.844189 | 8.818256e-59 | -8.615850e-47 |
| Msa0575740 | Msa0667850 | 0.814170 | 1.765970e-51 | -8.615850e-47 |
| Msa0591800 | Msa0667850 | 0.816735 | 4.745333e-52 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 61 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGCCTCCCCAATGAATA+AGG | 0.271513 | 4_4:-8560503 | Msa0667850:CDS |
| AGCTCATATGGAAGCTGTTA+TGG | 0.350210 | 4_4:-8560608 | Msa0667850:exon |
| AAGGAGTAATCCTTATTCAT+TGG | 0.357129 | 4_4:+8560493 | None:intergenic |
| AGGAGTAATCCTTATTCATT+GGG | 0.384870 | 4_4:+8560494 | None:intergenic |
| TCTGTCTCCGATGTTTGATA+TGG | 0.389125 | 4_4:-8560554 | Msa0667850:CDS |
| TTCAGCTGCACCAGCTCATA+TGG | 0.389169 | 4_4:-8560620 | Msa0667850:exon |
| CCTCAGATTCAATAGTTGTA+AGG | 0.410226 | 4_4:+8560084 | None:intergenic |
| ATATGAGCTGGTGCAGCTGA+AGG | 0.500074 | 4_4:+8560622 | None:intergenic |
| AATGAAGAGTACTGCTGATC+CGG | 0.523147 | 4_4:+8560156 | None:intergenic |
| GAAGGTGAGTACTACTGATC+CGG | 0.524957 | 4_4:+8560186 | None:intergenic |
| TTATGTAGCCGAGGGGTCAC+CGG | 0.530382 | 4_4:-8560205 | Msa0667850:intron |
| CTCAGATTCAATAGTTGTAA+GGG | 0.535368 | 4_4:+8560085 | None:intergenic |
| GTCTCCGATGTTTGATATGG+TGG | 0.541847 | 4_4:-8560551 | Msa0667850:CDS |
| TACCTCGGATACCACTGATC+TGG | 0.541909 | 4_4:+8560418 | None:intergenic |
| TATGAAGATGAGAGGTATGA+AGG | 0.546190 | 4_4:+8560448 | None:intergenic |
| GTACTCACCTTCACCGTCAC+CGG | 0.549143 | 4_4:-8560175 | Msa0667850:CDS |
| ATAACAGCTTCCATATGAGC+TGG | 0.571986 | 4_4:+8560610 | None:intergenic |
| GGATACCACTGATCTGGTGA+GGG | 0.584225 | 4_4:+8560424 | None:intergenic |
| TATGAGCTGGTGCAGCTGAA+GGG | 0.584297 | 4_4:+8560623 | None:intergenic |
| GGAGACAGAAATATCTTCCA+TGG | 0.596472 | 4_4:+8560568 | None:intergenic |
| CGGATACCACTGATCTGGTG+AGG | 0.600056 | 4_4:+8560423 | None:intergenic |
| AGGATTACTCCTTTGATCTG+AGG | 0.600461 | 4_4:-8560483 | Msa0667850:CDS |
| GGGGAGGCAACAAGTCTCCA+TGG | 0.603405 | 4_4:+8560514 | None:intergenic |
| CCTTACAACTATTGAATCTG+AGG | 0.607890 | 4_4:-8560084 | Msa0667850:CDS |
| CTACTGATCCGGTGACCCCT+CGG | 0.615519 | 4_4:+8560197 | None:intergenic |
| GTGAGGGGTATGAAGATGAG+AGG | 0.616209 | 4_4:+8560440 | None:intergenic |
| AGTTGTAAGGGCTCAAGAGA+CGG | 0.621176 | 4_4:+8560097 | None:intergenic |
| TCATCACCAAAATCAAGGAA+GGG | 0.624569 | 4_4:-8560657 | None:intergenic |
| GTAATCCTTATTCATTGGGG+AGG | 0.626219 | 4_4:+8560498 | None:intergenic |
| TGCTGATCCGGTGACGGTGA+AGG | 0.639909 | 4_4:+8560168 | None:intergenic |
| CACCAGATCAGTGGTATCCG+AGG | 0.640182 | 4_4:-8560420 | Msa0667850:intron |
| TGACGAGTACCTCAGATCAA+AGG | 0.642736 | 4_4:+8560474 | None:intergenic |
| GAGTATTGTTGATGCGATGA+CGG | 0.643808 | 4_4:+8560130 | None:intergenic |
| TCATATGGAAGCTGTTATGG+AGG | 0.648929 | 4_4:-8560605 | Msa0667850:exon |
| GAAGATGAGAGGTATGAAGG+TGG | 0.660006 | 4_4:+8560451 | None:intergenic |
| AAGGGCTCAAGAGACGGTGA+AGG | 0.662606 | 4_4:+8560103 | None:intergenic |
| CATACCCCTCACCAGATCAG+TGG | 0.663428 | 4_4:-8560429 | Msa0667850:CDS |
| TGTACCACCATATCAAACAT+CGG | 0.670313 | 4_4:+8560547 | None:intergenic |
| TGGTACAAACATCGTCACCA+TGG | 0.678551 | 4_4:-8560531 | Msa0667850:CDS |
| GAGTACTGCTGATCCGGTGA+CGG | 0.679194 | 4_4:+8560162 | None:intergenic |
| AGGCACATACATCGCCACCA+TGG | 0.693204 | 4_4:-8560585 | Msa0667850:CDS |
| GGAGTAATCCTTATTCATTG+GGG | 0.697509 | 4_4:+8560495 | None:intergenic |
| GATACCACTGATCTGGTGAG+GGG | 0.708796 | 4_4:+8560425 | None:intergenic |
| GACAGAAATATCTTCCATGG+TGG | 0.709754 | 4_4:+8560571 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTATTTTTAGTTGCACTTT+TGG | - | chr4_4:8560436-8560455 | Msa0667850:CDS | 20.0% |
| ! | TATAAGGACATAAATTACCT+CGG | + | chr4_4:8560299-8560318 | None:intergenic | 25.0% |
| AGGAGTAATCCTTATTCATT+GGG | + | chr4_4:8560208-8560227 | None:intergenic | 30.0% | |
| AAGGAGTAATCCTTATTCAT+TGG | + | chr4_4:8560209-8560228 | None:intergenic | 30.0% | |
| CAATGCCATAAAAAATGGTA+AGG | + | chr4_4:8560332-8560351 | None:intergenic | 30.0% | |
| CTCAGATTCAATAGTTGTAA+GGG | + | chr4_4:8560617-8560636 | None:intergenic | 30.0% | |
| TGTACCACCATATCAAACAT+CGG | + | chr4_4:8560155-8560174 | None:intergenic | 35.0% | |
| GGAGTAATCCTTATTCATTG+GGG | + | chr4_4:8560207-8560226 | None:intergenic | 35.0% | |
| ! | TATGAAGATGAGAGGTATGA+AGG | + | chr4_4:8560254-8560273 | None:intergenic | 35.0% |
| ATAAAAAATGGTAAGGAGCG+GGG | + | chr4_4:8560325-8560344 | None:intergenic | 35.0% | |
| CATAAAAAATGGTAAGGAGC+GGG | + | chr4_4:8560326-8560345 | None:intergenic | 35.0% | |
| CCATAAAAAATGGTAAGGAG+CGG | + | chr4_4:8560327-8560346 | None:intergenic | 35.0% | |
| CTGAGCAATGCCATAAAAAA+TGG | + | chr4_4:8560337-8560356 | None:intergenic | 35.0% | |
| TGGCTTGACCATTGTATTTA+TGG | - | chr4_4:8560456-8560475 | Msa0667850:CDS | 35.0% | |
| CCTCAGATTCAATAGTTGTA+AGG | + | chr4_4:8560618-8560637 | None:intergenic | 35.0% | |
| CCTTACAACTATTGAATCTG+AGG | - | chr4_4:8560615-8560634 | Msa0667850:exon | 35.0% | |
| ATAACAGCTTCCATATGAGC+TGG | + | chr4_4:8560092-8560111 | None:intergenic | 40.0% | |
| AGCTCATATGGAAGCTGTTA+TGG | - | chr4_4:8560091-8560110 | Msa0667850:CDS | 40.0% | |
| TCATATGGAAGCTGTTATGG+AGG | - | chr4_4:8560094-8560113 | Msa0667850:CDS | 40.0% | |
| GACAGAAATATCTTCCATGG+TGG | + | chr4_4:8560131-8560150 | None:intergenic | 40.0% | |
| GGAGACAGAAATATCTTCCA+TGG | + | chr4_4:8560134-8560153 | None:intergenic | 40.0% | |
| TCTGTCTCCGATGTTTGATA+TGG | - | chr4_4:8560145-8560164 | Msa0667850:CDS | 40.0% | |
| GTAATCCTTATTCATTGGGG+AGG | + | chr4_4:8560204-8560223 | None:intergenic | 40.0% | |
| AGGATTACTCCTTTGATCTG+AGG | - | chr4_4:8560216-8560235 | Msa0667850:intron | 40.0% | |
| !!! | CCGCTCCTTACCATTTTTTA+TGG | - | chr4_4:8560324-8560343 | Msa0667850:intron | 40.0% |
| ATTGCTCAGTGCATTAGAAC+AGG | - | chr4_4:8560348-8560367 | Msa0667850:intron | 40.0% | |
| GCATTAGAACAGGACATGTA+TGG | - | chr4_4:8560358-8560377 | Msa0667850:intron | 40.0% | |
| !! | CATGCTTTTTATGTAGCCGA+GGG | - | chr4_4:8560486-8560505 | Msa0667850:CDS | 40.0% |
| !! | ATGCTTTTTATGTAGCCGAG+GGG | - | chr4_4:8560487-8560506 | Msa0667850:CDS | 40.0% |
| !! | AATGAAGAGTACTGCTGATC+CGG | + | chr4_4:8560546-8560565 | None:intergenic | 40.0% |
| ! | GAGTATTGTTGATGCGATGA+CGG | + | chr4_4:8560572-8560591 | None:intergenic | 40.0% |
| GTCTCCGATGTTTGATATGG+TGG | - | chr4_4:8560148-8560167 | Msa0667850:CDS | 45.0% | |
| TGGTACAAACATCGTCACCA+TGG | - | chr4_4:8560168-8560187 | Msa0667850:CDS | 45.0% | |
| TGTTGCCTCCCCAATGAATA+AGG | - | chr4_4:8560196-8560215 | Msa0667850:CDS | 45.0% | |
| TGACGAGTACCTCAGATCAA+AGG | + | chr4_4:8560228-8560247 | None:intergenic | 45.0% | |
| ! | GAAGATGAGAGGTATGAAGG+TGG | + | chr4_4:8560251-8560270 | None:intergenic | 45.0% |
| GTAAGGAGCGGGGAAATATA+AGG | + | chr4_4:8560315-8560334 | None:intergenic | 45.0% | |
| GGTGGCTGCCATAAATACAA+TGG | + | chr4_4:8560467-8560486 | None:intergenic | 45.0% | |
| ! | CGGCTACATAAAAAGCATGG+TGG | + | chr4_4:8560485-8560504 | None:intergenic | 45.0% |
| CCTCGGCTACATAAAAAGCA+TGG | + | chr4_4:8560488-8560507 | None:intergenic | 45.0% | |
| !! | CCATGCTTTTTATGTAGCCG+AGG | - | chr4_4:8560485-8560504 | Msa0667850:CDS | 45.0% |
| GAAGGTGAGTACTACTGATC+CGG | + | chr4_4:8560516-8560535 | None:intergenic | 45.0% | |
| AGTTGTAAGGGCTCAAGAGA+CGG | + | chr4_4:8560605-8560624 | None:intergenic | 45.0% | |
| TATGAGCTGGTGCAGCTGAA+GGG | + | chr4_4:8560079-8560098 | None:intergenic | 50.0% | |
| ATATGAGCTGGTGCAGCTGA+AGG | + | chr4_4:8560080-8560099 | None:intergenic | 50.0% | |
| TTCAGCTGCACCAGCTCATA+TGG | - | chr4_4:8560079-8560098 | Msa0667850:CDS | 50.0% | |
| ! | GTGAGGGGTATGAAGATGAG+AGG | + | chr4_4:8560262-8560281 | None:intergenic | 50.0% |
| GATACCACTGATCTGGTGAG+GGG | + | chr4_4:8560277-8560296 | None:intergenic | 50.0% | |
| GGATACCACTGATCTGGTGA+GGG | + | chr4_4:8560278-8560297 | None:intergenic | 50.0% | |
| TACCTCGGATACCACTGATC+TGG | + | chr4_4:8560284-8560303 | None:intergenic | 50.0% | |
| AGGCACATACATCGCCACCA+TGG | - | chr4_4:8560114-8560133 | Msa0667850:CDS | 55.0% | |
| CATACCCCTCACCAGATCAG+TGG | - | chr4_4:8560270-8560289 | Msa0667850:intron | 55.0% | |
| CGGATACCACTGATCTGGTG+AGG | + | chr4_4:8560279-8560298 | None:intergenic | 55.0% | |
| CACCAGATCAGTGGTATCCG+AGG | - | chr4_4:8560279-8560298 | Msa0667850:intron | 55.0% | |
| TTATGTAGCCGAGGGGTCAC+CGG | - | chr4_4:8560494-8560513 | Msa0667850:CDS | 55.0% | |
| GTACTCACCTTCACCGTCAC+CGG | - | chr4_4:8560524-8560543 | Msa0667850:CDS | 55.0% | |
| ! | GAGTACTGCTGATCCGGTGA+CGG | + | chr4_4:8560540-8560559 | None:intergenic | 55.0% |
| AAGGGCTCAAGAGACGGTGA+AGG | + | chr4_4:8560599-8560618 | None:intergenic | 55.0% | |
| GGGGAGGCAACAAGTCTCCA+TGG | + | chr4_4:8560188-8560207 | None:intergenic | 60.0% | |
| CTACTGATCCGGTGACCCCT+CGG | + | chr4_4:8560505-8560524 | None:intergenic | 60.0% | |
| ! | TGCTGATCCGGTGACGGTGA+AGG | + | chr4_4:8560534-8560553 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4_4 | gene | 8560057 | 8560664 | 8560057 | ID=Msa0667850;Name=Msa0667850 |
| chr4_4 | mRNA | 8560057 | 8560664 | 8560057 | ID=Msa0667850-mRNA-1;Parent=Msa0667850;Name=Msa0667850-mRNA-1;_AED=0.06;_eAED=0.39;_QI=41|0|0.5|1|1|1|2|0|121 |
| chr4_4 | exon | 8560057 | 8560219 | 8560057 | ID=Msa0667850-mRNA-1:exon:2754;Parent=Msa0667850-mRNA-1 |
| chr4_4 | exon | 8560421 | 8560664 | 8560421 | ID=Msa0667850-mRNA-1:exon:2753;Parent=Msa0667850-mRNA-1 |
| chr4_4 | five_prime_UTR | 8560624 | 8560664 | 8560624 | ID=Msa0667850-mRNA-1:five_prime_utr;Parent=Msa0667850-mRNA-1 |
| chr4_4 | CDS | 8560421 | 8560623 | 8560421 | ID=Msa0667850-mRNA-1:cds;Parent=Msa0667850-mRNA-1 |
| chr4_4 | CDS | 8560057 | 8560219 | 8560057 | ID=Msa0667850-mRNA-1:cds;Parent=Msa0667850-mRNA-1 |
| Gene Sequence |
| Protein sequence |
