Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0709730 | XP_013462141.1 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 9.79e-136 | 387 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0709730 | sp|A4QND5|NIP7_XENTR | 61.497 | 187 | 64 | 1 | 1 | 187 | 1 | 179 | 1.02e-79 | 238 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0709730 | I3ST48 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 4.68e-136 | 387 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| Msa0002790 | Msa0709730 | 0.808925 | 2.437622e-50 | -8.615850e-47 |
| Msa0019390 | Msa0709730 | 0.865854 | 4.365256e-65 | -8.615850e-47 |
| Msa0022970 | Msa0709730 | 0.844838 | 5.898615e-59 | -8.615850e-47 |
| Msa0043660 | Msa0709730 | 0.813879 | 2.047288e-51 | -8.615850e-47 |
| Msa0044890 | Msa0709730 | 0.814373 | 1.592458e-51 | -8.615850e-47 |
| Msa0067630 | Msa0709730 | 0.815288 | 9.985051e-52 | -8.615850e-47 |
| Msa0068970 | Msa0709730 | 0.810716 | 1.003941e-50 | -8.615850e-47 |
| Msa0115130 | Msa0709730 | 0.819397 | 1.186506e-52 | -8.615850e-47 |
| Msa0118710 | Msa0709730 | 0.837764 | 4.287372e-57 | -8.615850e-47 |
| Msa0605000 | Msa0709730 | 0.809993 | 1.437296e-50 | -8.615850e-47 |
| Msa0648780 | Msa0709730 | 0.863801 | 1.919833e-64 | -8.615850e-47 |
| Msa0660850 | Msa0709730 | 0.831718 | 1.423742e-55 | -8.615850e-47 |
| Msa0704980 | Msa0709730 | 0.822203 | 2.685292e-53 | -8.615850e-47 |
| Msa0709730 | Msa0719420 | 0.851662 | 7.668276e-61 | -8.615850e-47 |
| Msa0709730 | Msa0719610 | 0.806947 | 6.420937e-50 | -8.615850e-47 |
| Msa0709730 | Msa0745740 | 0.802245 | 6.149032e-49 | -8.615850e-47 |
| Msa0709730 | Msa0757940 | 0.827223 | 1.761109e-54 | -8.615850e-47 |
| Msa0709730 | Msa0763330 | 0.830627 | 2.639850e-55 | -8.615850e-47 |
| Msa0709730 | Msa0772480 | 0.810888 | 9.212841e-51 | -8.615850e-47 |
| Msa0709730 | Msa0789680 | 0.852877 | 3.459179e-61 | -8.615850e-47 |
| Msa0709730 | Msa0790120 | 0.842561 | 2.399012e-58 | -8.615850e-47 |
| Msa0709730 | Msa0799260 | 0.839175 | 1.853629e-57 | -8.615850e-47 |
| Msa0709730 | Msa0799310 | 0.836879 | 7.223208e-57 | -8.615850e-47 |
| Msa0709730 | Msa0802410 | 0.832818 | 7.608885e-56 | -8.615850e-47 |
| Msa0709730 | Msa0806880 | 0.802810 | 4.700641e-49 | -8.615850e-47 |
| Msa0709730 | Msa0837020 | 0.805372 | 1.378181e-49 | -8.615850e-47 |
| Msa0709730 | Msa0837630 | 0.812330 | 4.475943e-51 | -8.615850e-47 |
| Msa0709730 | Msa0856530 | 0.812584 | 3.938619e-51 | -8.615850e-47 |
| Msa0709730 | Msa0869130 | 0.841243 | 5.346380e-58 | -8.615850e-47 |
| Msa0709730 | Msa0879540 | 0.817336 | 3.477020e-52 | -8.615850e-47 |
| Msa0709730 | Msa0930490 | 0.857031 | 2.152023e-62 | -8.615850e-47 |
| Msa0709730 | Msa0933300 | 0.866619 | 2.497547e-65 | -8.615850e-47 |
| Msa0709730 | Msa1027640 | 0.826745 | 2.291264e-54 | -8.615850e-47 |
| Msa0709730 | Msa1066190 | 0.828173 | 1.041374e-54 | -8.615850e-47 |
| Msa0709730 | Msa1088490 | 0.812644 | 3.821174e-51 | -8.615850e-47 |
| Msa0709730 | Msa1153060 | 0.820561 | 6.425809e-53 | -8.615850e-47 |
| Msa0709730 | Msa1157580 | 0.813579 | 2.383071e-51 | -8.615850e-47 |
| Msa0709730 | Msa1191830 | 0.818201 | 2.217649e-52 | -8.615850e-47 |
| Msa0709730 | Msa1209260 | 0.841614 | 4.270630e-58 | -8.615850e-47 |
| Msa0709730 | Msa1260530 | 0.807588 | 4.698224e-50 | -8.615850e-47 |
| Msa0709730 | Msa1356400 | 0.825342 | 4.940559e-54 | -8.615850e-47 |
| Msa0709730 | Msa1366180 | 0.817200 | 3.729493e-52 | -8.615850e-47 |
| Msa0709730 | Msa1387410 | 0.818531 | 1.866983e-52 | -8.615850e-47 |
| Msa0709730 | Msa1413500 | 0.811951 | 5.414750e-51 | -8.615850e-47 |
| Msa0137550 | Msa0709730 | 0.807598 | 4.674429e-50 | -8.615850e-47 |
| Msa0158000 | Msa0709730 | 0.803143 | 4.012577e-49 | -8.615850e-47 |
| Msa0159170 | Msa0709730 | 0.804793 | 1.821623e-49 | -8.615850e-47 |
| Msa0164770 | Msa0709730 | 0.819418 | 1.173749e-52 | -8.615850e-47 |
| Msa0189300 | Msa0709730 | 0.821688 | 3.534205e-53 | -8.615850e-47 |
| Msa0231030 | Msa0709730 | 0.836511 | 8.963844e-57 | -8.615850e-47 |
| Msa0231660 | Msa0709730 | 0.815141 | 1.076203e-51 | -8.615850e-47 |
| Msa0421500 | Msa0709730 | 0.805638 | 1.212204e-49 | -8.615850e-47 |
| Msa0482130 | Msa0709730 | 0.812853 | 3.439522e-51 | -8.615850e-47 |
| Msa0270550 | Msa0709730 | 0.826388 | 2.787786e-54 | -8.615850e-47 |
| Msa0289580 | Msa0709730 | 0.802080 | 6.648182e-49 | -8.615850e-47 |
| Msa0289610 | Msa0709730 | 0.809512 | 1.824514e-50 | -8.615850e-47 |
| Msa0325590 | Msa0709730 | 0.812401 | 4.318934e-51 | -8.615850e-47 |
| Msa0327300 | Msa0709730 | 0.815326 | 9.789570e-52 | -8.615850e-47 |
| Msa0485510 | Msa0709730 | 0.811537 | 6.664237e-51 | -8.615850e-47 |
| Msa0486070 | Msa0709730 | 0.826500 | 2.622689e-54 | -8.615850e-47 |
| Msa0581280 | Msa0709730 | 0.800157 | 1.643815e-48 | -8.615850e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0709730 | MtrunA17_Chr3g0141781 | 99.465 | 187 | 1 | 0 | 1 | 187 | 1 | 187 | 9.01e-140 | 387 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Msa0709730 | AT4G15770.1 | 83.422 | 187 | 31 | 0 | 1 | 187 | 1 | 187 | 3.92e-119 | 335 |
Find 46 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCGGATGTGCCTTTGGGTTT+TGG | 0.195441 | 5_1:-876454 | Msa0709730:CDS |
| TTCTATGTCGGATGTGCCTT+TGG | 0.274575 | 5_1:-876461 | Msa0709730:CDS |
| TGGTATTGTTGTGCTTCATC+AGG | 0.357825 | 5_1:-876386 | Msa0709730:CDS |
| GTGCTTCATCAGGGTGATAT+TGG | 0.380581 | 5_1:-876376 | Msa0709730:CDS |
| TCTATGTCGGATGTGCCTTT+GGG | 0.403032 | 5_1:-876460 | Msa0709730:CDS |
| GTGATATTGGAGAGTATTTG+AGG | 0.418524 | 5_1:-876363 | Msa0709730:CDS |
| CCAAGTCTACACAGGATTGT+AGG | 0.422709 | 5_1:-876423 | Msa0709730:CDS |
| GAGTGCGTTGGGTCGCATTA+CGG | 0.440043 | 5_1:-876518 | Msa0709730:CDS |
| CTCGTTTCTGTCTCGTCCAA+AGG | 0.450814 | 5_1:+876880 | None:intergenic |
| TGGAGAGTATTTGAGGATTG+AGG | 0.451876 | 5_1:-876356 | Msa0709730:CDS |
| ATTACGGAGAATATTGTTGC+TGG | 0.453018 | 5_1:-876502 | Msa0709730:CDS |
| AGAATATTGTTGCTGGTGAT+GGG | 0.475550 | 5_1:-876495 | Msa0709730:CDS |
| GAGAATATTGTTGCTGGTGA+TGG | 0.477429 | 5_1:-876496 | Msa0709730:CDS |
| TGAAGCACAACAATACCATT+AGG | 0.481187 | 5_1:+876391 | None:intergenic |
| GTTCCGAGGGAAACGAGGTT+AGG | 0.488759 | 5_1:+876682 | None:intergenic |
| TACACAGGATTGTAGGAAGT+TGG | 0.488772 | 5_1:-876416 | Msa0709730:CDS |
| GTAGCGACCAGGATTTGAAT+CGG | 0.501537 | 5_1:+876780 | None:intergenic |
| GTTTCCCTCGGAACGTGTAT+CGG | 0.505430 | 5_1:-876673 | Msa0709730:CDS |
| TGTAGGAAGTTGGATCCTAA+TGG | 0.511689 | 5_1:-876406 | Msa0709730:CDS |
| TTTAAGCCATACCTTATGCT+TGG | 0.513488 | 5_1:+876585 | None:intergenic |
| TGCTGCGAATGCCAAGCATA+AGG | 0.516412 | 5_1:-876596 | Msa0709730:CDS |
| GAAGGTCCCGATTCAAATCC+TGG | 0.525646 | 5_1:-876787 | Msa0709730:CDS |
| CCTACAATCCTGTGTAGACT+TGG | 0.530107 | 5_1:+876423 | None:intergenic |
| GAATCGGGACCTTCGTGTGA+GGG | 0.536023 | 5_1:+876796 | None:intergenic |
| AGACCTAACCTCGTTTCCCT+CGG | 0.540105 | 5_1:-876685 | Msa0709730:CDS |
| GAGGTTAGGTCTAGCGATGT+TGG | 0.552615 | 5_1:+876696 | None:intergenic |
| CATGTGTTGAAGAGTGCGTT+GGG | 0.555269 | 5_1:-876529 | Msa0709730:CDS |
| TAGCGACCAGGATTTGAATC+GGG | 0.568635 | 5_1:+876781 | None:intergenic |
| GAAAAGCTCTTCAAATTCGT+AGG | 0.571649 | 5_1:-876847 | Msa0709730:CDS |
| CGAATGCCAAGCATAAGGTA+TGG | 0.574051 | 5_1:-876591 | Msa0709730:CDS |
| TCATGTGTTGAAGAGTGCGT+TGG | 0.587350 | 5_1:-876530 | Msa0709730:CDS |
| AGACGGAAACAGTAGCGACC+AGG | 0.591481 | 5_1:+876769 | None:intergenic |
| GGTATTGTTGTGCTTCATCA+GGG | 0.595232 | 5_1:-876385 | Msa0709730:CDS |
| TGTTGGTGGCACGCTTAACG+AGG | 0.595506 | 5_1:+876713 | None:intergenic |
| GTTAGGTCTAGCGATGTTGG+TGG | 0.599334 | 5_1:+876699 | None:intergenic |
| TACACGTTCCGAGGGAAACG+AGG | 0.615959 | 5_1:+876677 | None:intergenic |
| TGAATCGGGACCTTCGTGTG+AGG | 0.619897 | 5_1:+876795 | None:intergenic |
| GCAGCCACCCCAAAACCCAA+AGG | 0.624108 | 5_1:+876445 | None:intergenic |
| AAGTAAATTCAACGCCTGAA+CGG | 0.628881 | 5_1:+876618 | None:intergenic |
| GAATATTGTTGCTGGTGATG+GGG | 0.643723 | 5_1:-876494 | Msa0709730:CDS |
| GTTGGTGGCACGCTTAACGA+GGG | 0.651920 | 5_1:+876714 | None:intergenic |
| GGTGGCTGCCAAGTCTACAC+AGG | 0.664800 | 5_1:-876431 | Msa0709730:CDS |
| GTTGAAAATCCCTCACACGA+AGG | 0.698320 | 5_1:-876805 | Msa0709730:CDS |
| GGACGAGACAGAAACGAGCG+TGG | 0.721090 | 5_1:-876875 | Msa0709730:CDS |
| TGTATCGGAAAATACACACA+CGG | 0.738380 | 5_1:-876658 | Msa0709730:CDS |
| ATCGGAAAATACACACACGG+TGG | 0.745885 | 5_1:-876655 | Msa0709730:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AGATTTTGTTCTTGTTGAGA+CGG | + | chr5_1:876482-876501 | None:intergenic | 30.0% |
| AAAAAAGACATCTCAGATTG+TGG | + | chr5_1:876672-876691 | None:intergenic | 30.0% | |
| !!! | TCTGAGATGTCTTTTTTGTA+TGG | - | chr5_1:876675-876694 | Msa0709730:CDS | 30.0% |
| !!! | CTGAGATGTCTTTTTTGTAT+GGG | - | chr5_1:876676-876695 | Msa0709730:CDS | 30.0% |
| !!! | GGTTGTTGTTTTTTCTATGT+CGG | - | chr5_1:876758-876777 | Msa0709730:CDS | 30.0% |
| GAAAAGCTCTTCAAATTCGT+AGG | - | chr5_1:876384-876403 | Msa0709730:CDS | 35.0% | |
| TGTATCGGAAAATACACACA+CGG | - | chr5_1:876573-876592 | Msa0709730:CDS | 35.0% | |
| ! | AAATTTTCATCTCACCGTTC+AGG | - | chr5_1:876599-876618 | Msa0709730:CDS | 35.0% |
| AAGTAAATTCAACGCCTGAA+CGG | + | chr5_1:876616-876635 | None:intergenic | 35.0% | |
| TTTAAGCCATACCTTATGCT+TGG | + | chr5_1:876649-876668 | None:intergenic | 35.0% | |
| ATTACGGAGAATATTGTTGC+TGG | - | chr5_1:876729-876748 | Msa0709730:CDS | 35.0% | |
| AGAATATTGTTGCTGGTGAT+GGG | - | chr5_1:876736-876755 | Msa0709730:CDS | 35.0% | |
| TGAAGCACAACAATACCATT+AGG | + | chr5_1:876843-876862 | None:intergenic | 35.0% | |
| !! | GTGATATTGGAGAGTATTTG+AGG | - | chr5_1:876868-876887 | Msa0709730:CDS | 35.0% |
| GAGAATATTGTTGCTGGTGA+TGG | - | chr5_1:876735-876754 | Msa0709730:CDS | 40.0% | |
| ! | GAATATTGTTGCTGGTGATG+GGG | - | chr5_1:876737-876756 | Msa0709730:CDS | 40.0% |
| TACACAGGATTGTAGGAAGT+TGG | - | chr5_1:876815-876834 | Msa0709730:CDS | 40.0% | |
| TGTAGGAAGTTGGATCCTAA+TGG | - | chr5_1:876825-876844 | Msa0709730:CDS | 40.0% | |
| TGGTATTGTTGTGCTTCATC+AGG | - | chr5_1:876845-876864 | Msa0709730:CDS | 40.0% | |
| GGTATTGTTGTGCTTCATCA+GGG | - | chr5_1:876846-876865 | Msa0709730:CDS | 40.0% | |
| !! | TGGAGAGTATTTGAGGATTG+AGG | - | chr5_1:876875-876894 | Msa0709730:CDS | 40.0% |
| GTTGAAAATCCCTCACACGA+AGG | - | chr5_1:876426-876445 | Msa0709730:CDS | 45.0% | |
| TAGCGACCAGGATTTGAATC+GGG | + | chr5_1:876453-876472 | None:intergenic | 45.0% | |
| GTAGCGACCAGGATTTGAAT+CGG | + | chr5_1:876454-876473 | None:intergenic | 45.0% | |
| ! | TTTTCCGATACACGTTCCGA+GGG | + | chr5_1:876565-876584 | None:intergenic | 45.0% |
| ! | ATTTTCCGATACACGTTCCG+AGG | + | chr5_1:876566-876585 | None:intergenic | 45.0% |
| ATCGGAAAATACACACACGG+TGG | - | chr5_1:876576-876595 | Msa0709730:CDS | 45.0% | |
| CGAATGCCAAGCATAAGGTA+TGG | - | chr5_1:876640-876659 | Msa0709730:CDS | 45.0% | |
| ! | TCATGTGTTGAAGAGTGCGT+TGG | - | chr5_1:876701-876720 | Msa0709730:CDS | 45.0% |
| ! | CATGTGTTGAAGAGTGCGTT+GGG | - | chr5_1:876702-876721 | Msa0709730:CDS | 45.0% |
| TTCTATGTCGGATGTGCCTT+TGG | - | chr5_1:876770-876789 | Msa0709730:CDS | 45.0% | |
| TCTATGTCGGATGTGCCTTT+GGG | - | chr5_1:876771-876790 | Msa0709730:CDS | 45.0% | |
| CCTACAATCCTGTGTAGACT+TGG | + | chr5_1:876811-876830 | None:intergenic | 45.0% | |
| CCAAGTCTACACAGGATTGT+AGG | - | chr5_1:876808-876827 | Msa0709730:CDS | 45.0% | |
| GTGCTTCATCAGGGTGATAT+TGG | - | chr5_1:876855-876874 | Msa0709730:CDS | 45.0% | |
| CTCGTTTCTGTCTCGTCCAA+AGG | + | chr5_1:876354-876373 | None:intergenic | 50.0% | |
| ! | CGTGTGAGGGATTTTCAACG+AGG | + | chr5_1:876425-876444 | None:intergenic | 50.0% |
| GAAGGTCCCGATTCAAATCC+TGG | - | chr5_1:876444-876463 | Msa0709730:CDS | 50.0% | |
| GTTAGGTCTAGCGATGTTGG+TGG | + | chr5_1:876535-876554 | None:intergenic | 50.0% | |
| GAGGTTAGGTCTAGCGATGT+TGG | + | chr5_1:876538-876557 | None:intergenic | 50.0% | |
| AGACCTAACCTCGTTTCCCT+CGG | - | chr5_1:876546-876565 | Msa0709730:CDS | 50.0% | |
| GTTTCCCTCGGAACGTGTAT+CGG | - | chr5_1:876558-876577 | Msa0709730:CDS | 50.0% | |
| TGCTGCGAATGCCAAGCATA+AGG | - | chr5_1:876635-876654 | Msa0709730:CDS | 50.0% | |
| !! | TCGGATGTGCCTTTGGGTTT+TGG | - | chr5_1:876777-876796 | Msa0709730:CDS | 50.0% |
| !! | CGGATGTGCCTTTGGGTTTT+GGG | - | chr5_1:876778-876797 | Msa0709730:CDS | 50.0% |
| !! | GGATGTGCCTTTGGGTTTTG+GGG | - | chr5_1:876779-876798 | Msa0709730:CDS | 50.0% |
| GAATCGGGACCTTCGTGTGA+GGG | + | chr5_1:876438-876457 | None:intergenic | 55.0% | |
| TGAATCGGGACCTTCGTGTG+AGG | + | chr5_1:876439-876458 | None:intergenic | 55.0% | |
| AGACGGAAACAGTAGCGACC+AGG | + | chr5_1:876465-876484 | None:intergenic | 55.0% | |
| !! | GTTGGTGGCACGCTTAACGA+GGG | + | chr5_1:876520-876539 | None:intergenic | 55.0% |
| !! | TGTTGGTGGCACGCTTAACG+AGG | + | chr5_1:876521-876540 | None:intergenic | 55.0% |
| GTTCCGAGGGAAACGAGGTT+AGG | + | chr5_1:876552-876571 | None:intergenic | 55.0% | |
| TACACGTTCCGAGGGAAACG+AGG | + | chr5_1:876557-876576 | None:intergenic | 55.0% | |
| GAGTGCGTTGGGTCGCATTA+CGG | - | chr5_1:876713-876732 | Msa0709730:CDS | 55.0% | |
| !!! | TGTGCCTTTGGGTTTTGGGG+TGG | - | chr5_1:876782-876801 | Msa0709730:CDS | 55.0% |
| GGACGAGACAGAAACGAGCG+TGG | - | chr5_1:876356-876375 | Msa0709730:CDS | 60.0% | |
| GCAGCCACCCCAAAACCCAA+AGG | + | chr5_1:876789-876808 | None:intergenic | 60.0% | |
| GGTGGCTGCCAAGTCTACAC+AGG | - | chr5_1:876800-876819 | Msa0709730:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5_1 | gene | 876345 | 876908 | 876345 | ID=Msa0709730;Name=Msa0709730 |
| chr5_1 | mRNA | 876345 | 876908 | 876345 | ID=Msa0709730-mRNA-1;Parent=Msa0709730;Name=Msa0709730-mRNA-1;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|1|1|0|187 |
| chr5_1 | exon | 876345 | 876908 | 876345 | ID=Msa0709730-mRNA-1:exon:422;Parent=Msa0709730-mRNA-1 |
| chr5_1 | CDS | 876345 | 876908 | 876345 | ID=Msa0709730-mRNA-1:cds;Parent=Msa0709730-mRNA-1 |
| Gene Sequence |
| Protein sequence |
