AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00286

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00286.t1 MTR_2g436460 66.404 381 56 4 13 323 1 379 3.02e-161 455
MS.gene00286.t1 MTR_2g437030 40.885 384 151 8 13 323 79 459 7.95e-82 256
MS.gene00286.t1 MTR_2g437040 42.500 360 130 7 35 323 20 373 9.07e-82 253
MS.gene00286.t1 MTR_2g437020 40.710 366 142 8 30 323 11 373 9.21e-78 243
MS.gene00286.t1 MTR_2g437170 40.263 380 146 8 22 321 13 391 1.22e-76 241
MS.gene00286.t1 MTR_2g437940 43.732 343 138 7 28 321 19 355 4.58e-76 238
MS.gene00286.t1 MTR_2g437150 39.628 376 146 8 28 323 19 393 2.16e-75 237
MS.gene00286.t1 MTR_2g436440 41.279 344 131 7 50 323 37 379 3.52e-74 233
MS.gene00286.t1 MTR_2g437160 46.909 275 122 4 51 323 55 307 4.11e-72 226
MS.gene00286.t1 MTR_2g437260 38.329 347 131 5 51 321 28 367 1.51e-71 227
MS.gene00286.t1 MTR_2g437080 36.132 393 159 8 1 323 3 373 1.65e-69 221
MS.gene00286.t1 MTR_2g437130 39.883 341 130 5 54 323 38 374 7.39e-69 220
MS.gene00286.t1 MTR_2g437180 39.402 368 133 9 36 321 22 381 1.67e-68 219
MS.gene00286.t1 MTR_2g076320 38.983 354 140 6 46 323 33 386 1.12e-67 217
MS.gene00286.t1 MTR_2g436400 36.011 361 154 6 39 323 21 380 2.50e-67 216
MS.gene00286.t1 MTR_2g437100 37.867 375 150 8 21 320 29 395 7.08e-67 215
MS.gene00286.t1 MTR_2g437990 39.886 351 136 6 46 321 33 383 5.34e-66 214
MS.gene00286.t1 MTR_2g438010 39.437 355 133 7 46 321 53 404 1.08e-65 213
MS.gene00286.t1 MTR_4g119550 36.337 344 141 6 52 323 46 383 4.93e-65 210
MS.gene00286.t1 MTR_2g437960 39.286 336 124 6 61 320 55 386 5.56e-65 210
MS.gene00286.t1 MTR_2g437120 37.887 388 151 10 19 321 2 384 7.16e-65 210
MS.gene00286.t1 MTR_2g437960 39.286 336 124 6 61 320 55 386 8.67e-65 210
MS.gene00286.t1 MTR_2g437060 34.103 390 183 7 4 323 3 388 4.60e-64 207
MS.gene00286.t1 MTR_2g437160 37.752 347 139 5 51 323 55 398 1.03e-63 207
MS.gene00286.t1 MTR_4g119580 35.435 333 144 5 54 317 39 369 3.54e-59 194
MS.gene00286.t1 MTR_2g019810 37.425 334 136 6 57 320 29 359 5.67e-58 191
MS.gene00286.t1 MTR_2g437200 41.634 257 114 2 99 320 143 398 1.38e-56 188
MS.gene00286.t1 MTR_2g437200 54.783 115 52 0 51 165 24 138 3.57e-33 126
MS.gene00286.t1 MTR_4g120380 35.593 354 152 7 36 317 20 369 2.23e-56 187
MS.gene00286.t1 MTR_2g437240 42.308 260 108 4 99 320 143 398 1.21e-55 186
MS.gene00286.t1 MTR_2g437240 55.856 111 49 0 55 165 28 138 7.66e-32 123
MS.gene00286.t1 MTR_2g438020 47.500 200 99 3 124 321 243 438 4.03e-52 179
MS.gene00286.t1 MTR_2g438020 55.238 105 47 0 61 165 73 177 2.15e-28 114
MS.gene00286.t1 MTR_4g120380 35.224 335 141 7 36 298 20 350 7.50e-51 174
MS.gene00286.t1 MTR_4g120380 26.562 192 115 3 93 284 250 415 2.61e-14 73.6
MS.gene00286.t1 MTR_2g437090 49.342 152 76 1 172 323 11 161 5.98e-47 156
MS.gene00286.t1 MTR_2g019840 34.311 341 155 11 51 323 35 374 8.46e-43 152
MS.gene00286.t1 MTR_3g085240 34.354 294 125 8 19 311 22 248 3.15e-36 131
MS.gene00286.t1 MTR_2g436370 46.763 139 66 2 39 169 20 158 5.79e-36 129
MS.gene00286.t1 MTR_3g450500 29.217 332 158 7 61 317 40 369 9.60e-33 125
MS.gene00286.t1 MTR_4g007490 26.648 349 180 6 44 317 20 367 1.96e-30 119
MS.gene00286.t1 MTR_4g119570 34.247 219 106 5 98 316 47 227 1.20e-28 110
MS.gene00286.t1 MTR_4g049400 42.735 117 61 2 46 156 33 149 4.12e-18 82.4
MS.gene00286.t1 MTR_8g080990 23.774 265 160 5 87 314 109 368 1.73e-15 76.6
MS.gene00286.t1 MTR_5g041630 28.495 186 128 4 94 275 91 275 1.81e-15 76.3
MS.gene00286.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.18e-14 73.6
MS.gene00286.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.51e-14 73.6
MS.gene00286.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.86e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00286.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene00286.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene00286.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene00286.t1 AT1G21150 26.420 352 183 6 40 315 33 384 1.68e-32 124
MS.gene00286.t1 AT1G21150 26.420 352 183 6 40 315 97 448 5.22e-32 124
MS.gene00286.t1 AT5G07900 33.333 216 141 3 105 318 186 400 2.02e-31 122
MS.gene00286.t1 AT5G07900 31.818 132 89 1 38 168 46 177 1.10e-16 80.5
MS.gene00286.t1 AT1G21150 25.294 340 181 5 49 315 9 348 2.63e-31 120
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene00286.t1 AT1G61990 25.895 363 156 9 61 317 53 408 1.42e-22 97.8
MS.gene00286.t1 AT1G61990 25.895 363 156 9 61 317 53 408 1.42e-22 97.8
MS.gene00286.t1 AT1G62120 42.105 114 66 0 44 157 40 153 4.11e-22 96.7
MS.gene00286.t1 AT1G62120 29.412 170 83 4 171 304 238 406 1.14e-11 65.9
MS.gene00286.t1 AT3G46950 38.621 145 77 2 25 164 19 156 7.65e-22 95.9
MS.gene00286.t1 AT1G62085 41.346 104 61 0 61 164 56 159 1.33e-19 89.4
MS.gene00286.t1 AT1G62085 22.822 241 148 5 67 304 235 440 4.84e-11 63.9
MS.gene00286.t1 AT1G62085 41.346 104 61 0 61 164 56 159 1.33e-19 89.4
MS.gene00286.t1 AT1G62085 22.822 241 148 5 67 304 235 440 4.84e-11 63.9
MS.gene00286.t1 AT1G61960 39.450 109 66 0 61 169 53 161 7.17e-19 87.4
MS.gene00286.t1 AT1G61960 22.989 261 161 5 67 322 231 456 1.06e-13 72.0
MS.gene00286.t1 AT5G23930 39.316 117 71 0 41 157 36 152 1.58e-18 86.3
MS.gene00286.t1 AT1G62110 39.167 120 72 1 39 157 30 149 3.02e-18 85.5
MS.gene00286.t1 AT1G62150 39.423 104 63 0 61 164 59 162 2.13e-17 82.8
MS.gene00286.t1 AT1G62150 29.208 202 131 7 126 323 263 456 1.57e-12 68.6
MS.gene00286.t1 AT5G64950 28.986 207 136 5 119 317 172 375 2.90e-15 76.3
MS.gene00286.t1 AT1G56380 29.187 209 116 3 57 234 24 231 2.66e-14 73.6
MS.gene00286.t1 AT1G56380 29.187 209 116 3 57 234 13 220 2.93e-14 73.2
MS.gene00286.t1 AT1G62010 30.769 169 80 4 171 303 216 383 3.62e-14 73.2

Find 69 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATAAGGTGATGAGATTT+TGG 0.153456 2.2:+52653984 MS.gene00286:CDS
ACAAGAAGCCCTAGAATTCT+TGG 0.255383 2.2:+52653533 MS.gene00286:CDS
AGACTTGAACCAAGAATTCT+AGG 0.272133 2.2:-52653542 None:intergenic
TTCTGATGAGAAACCGAAAT+TGG 0.280006 2.2:-52653288 None:intergenic
TCATATCTCATTACCAATTT+CGG 0.283798 2.2:+52653275 MS.gene00286:CDS
TGATTTCGACATGGCCCTTT+TGG 0.313754 2.2:-52653852 None:intergenic
AAAGAGGGGTTTGAAGCTTA+AGG 0.317138 2.2:-52653132 None:intergenic
TTTCGTATAGTTTGGAGAAT+AGG 0.328948 2.2:+52654059 MS.gene00286:CDS
AAAGAGTAGAAATGAACTTT+GGG 0.332780 2.2:-52653193 None:intergenic
GTATTGTTGTGGTTCGATTT+AGG 0.332858 2.2:-52653160 None:intergenic
ATTGAATTGTCTACTCCATT+TGG 0.352685 2.2:+52654142 MS.gene00286:CDS
TTCGTATAGTTTGGAGAATA+GGG 0.359509 2.2:+52654060 MS.gene00286:CDS
AAGTTGTTGTCTTTAAGAAA+TGG 0.363837 2.2:+52653888 MS.gene00286:CDS
ATCCACGGAGAAAATTAATA+AGG 0.367860 2.2:+52653970 MS.gene00286:CDS
TTGGGTCAATGAGTTGGGTT+GGG 0.371128 2.2:+52654003 MS.gene00286:CDS
AGTTGTTGTCTTTAAGAAAT+GGG 0.373623 2.2:+52653889 MS.gene00286:CDS
TTGGATTTGGTTAAGGAAAT+GGG 0.379428 2.2:+52653785 MS.gene00286:intron
TTCAGAGGCACAACAAATCT+TGG 0.379614 2.2:+52653090 MS.gene00286:CDS
GAAAGAGTAGAAATGAACTT+TGG 0.387896 2.2:-52653194 None:intergenic
AAATGGTTCTTGAAGCGTTT+AGG 0.395855 2.2:+52653924 MS.gene00286:CDS
TGAGTTGGGTTGGGACTCTT+CGG 0.410843 2.2:+52654012 MS.gene00286:CDS
CTTCTTACCGATTCAAATGT+AGG 0.416369 2.2:-52653581 None:intergenic
GGGGTCACATTTAAGAATGT+TGG 0.425752 2.2:-52653435 None:intergenic
TCAAGCATTGTTAGCCAAAA+GGG 0.433648 2.2:+52653838 MS.gene00286:CDS
GTCAAGCATTGTTAGCCAAA+AGG 0.438054 2.2:+52653837 MS.gene00286:CDS
TTAAATGTGACCCCTACAAA+AGG 0.439736 2.2:+52653444 MS.gene00286:CDS
TGAGATATGAAACTGTGAAC+TGG 0.448214 2.2:-52653262 None:intergenic
AGGAGGAGTGGTTCAAAGAG+AGG 0.463557 2.2:-52653220 None:intergenic
ATGAACTTTGGGGTTGGAAA+GGG 0.467912 2.2:-52653182 None:intergenic
GGGTCACATTTAAGAATGTT+GGG 0.468503 2.2:-52653434 None:intergenic
GGTTTGTTGTGAAGTGGTTG+AGG 0.469829 2.2:-52653240 None:intergenic
ACTCTTCGGCTCTTGTCAAA+AGG 0.469912 2.2:+52654026 MS.gene00286:CDS
TGCTTCAAATATTGATATTG+TGG 0.478150 2.2:+52653502 MS.gene00286:CDS
TCGATTTAGGTTGAGAAAGA+GGG 0.479858 2.2:-52653147 None:intergenic
TTCTTACCGATTCAAATGTA+GGG 0.480837 2.2:-52653580 None:intergenic
TTCTTGAAGCGTTTAGGAAG+CGG 0.487125 2.2:+52653930 MS.gene00286:CDS
TTTGGGTCAATGAGTTGGGT+TGG 0.489140 2.2:+52654002 MS.gene00286:CDS
GGCCATGTCGAAATCACGTT+GGG 0.495859 2.2:+52653859 MS.gene00286:CDS
AAAACAGCGATTACTGAGTC+GGG 0.496874 2.2:-52653359 None:intergenic
TTCGATTTAGGTTGAGAAAG+AGG 0.496950 2.2:-52653148 None:intergenic
ATGGGGTTGGTCTGATGAAA+TGG 0.499033 2.2:+52653907 MS.gene00286:CDS
TATAATCCCTACATTTGAAT+CGG 0.504738 2.2:+52653574 MS.gene00286:CDS
GGAGGAGTGGTTCAAAGAGA+GGG 0.505452 2.2:-52653219 None:intergenic
GTAGAAATGAACTTTGGGGT+TGG 0.510973 2.2:-52653188 None:intergenic
GTTGTTGTCTTTAAGAAATG+GGG 0.513781 2.2:+52653890 MS.gene00286:CDS
AATGAACTTTGGGGTTGGAA+AGG 0.516771 2.2:-52653183 None:intergenic
TAAATGTGACCCCTACAAAA+GGG 0.524191 2.2:+52653445 MS.gene00286:CDS
TGCGAAACAACACAAGCCCT+TGG 0.528307 2.2:-52654088 None:intergenic
TTCGGCTCTTGTCAAAAGGC+CGG 0.528513 2.2:+52654030 MS.gene00286:CDS
GAAAACAGCGATTACTGAGT+CGG 0.530805 2.2:-52653360 None:intergenic
GGTTGGAAAGGGTATTGTTG+TGG 0.539751 2.2:-52653171 None:intergenic
TGGAGAATAGGGTCATTCCA+AGG 0.540810 2.2:+52654071 MS.gene00286:CDS
AAGCTTAAGGATTTCAACAT+CGG 0.550353 2.2:-52653119 None:intergenic
TTGTTGTGAAGTGGTTGAGG+AGG 0.551983 2.2:-52653237 None:intergenic
TTTCAATCTTGATGTTTCAG+AGG 0.555969 2.2:+52653075 None:intergenic
CGATTTAGGTTGAGAAAGAG+GGG 0.559408 2.2:-52653146 None:intergenic
TTGTCTTTAAGAAATGGGGT+TGG 0.562623 2.2:+52653894 MS.gene00286:CDS
GTGAAGTGGTTGAGGAGGAG+TGG 0.563267 2.2:-52653232 None:intergenic
ATTGTGTATGTTAGCATCCA+CGG 0.574891 2.2:+52653955 MS.gene00286:CDS
GGAGAATAGGGTCATTCCAA+GGG 0.578298 2.2:+52654072 MS.gene00286:CDS
GACTTGAACCAAGAATTCTA+GGG 0.579810 2.2:-52653541 None:intergenic
GGCTCTTGTCAAAAGGCCGG+AGG 0.586864 2.2:+52654033 MS.gene00286:CDS
GTTTCGCATTTGATCTCCAG+AGG 0.592319 2.2:+52654103 MS.gene00286:CDS
GAACTGGGTTTGTTGTGAAG+TGG 0.592348 2.2:-52653246 None:intergenic
GAGATATGAAACTGTGAACT+GGG 0.635008 2.2:-52653261 None:intergenic
AAGAGTAGAAATGAACTTTG+GGG 0.644547 2.2:-52653192 None:intergenic
GGGCCATGTCGAAATCACGT+TGG 0.650039 2.2:+52653858 MS.gene00286:CDS
ATCCCAACGTGATTTCGACA+TGG 0.666340 2.2:-52653861 None:intergenic
GTGGATGCTAACATACACAA+TGG 0.700210 2.2:-52653953 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAATTTTTAACTTCAAA+AGG + chr2.2:52653479-52653498 MS.gene00286:CDS 15.0%
!! GAAGTTAAAAATTCAAACTT+TGG - chr2.2:52653476-52653495 None:intergenic 20.0%
!! TCATAAGTCAATAATATAGA+CGG - chr2.2:52653752-52653771 None:intergenic 20.0%
!!! TATTATTGACTTATGATATG+AGG + chr2.2:52653756-52653775 MS.gene00286:intron 20.0%
! AAAGAGTAGAAATGAACTTT+GGG - chr2.2:52653196-52653215 None:intergenic 25.0%
! AAAGCTATACCTAGAAAAAA+TGG + chr2.2:52654231-52654250 MS.gene00286:CDS 25.0%
! AAGCTATACCTAGAAAAAAT+GGG + chr2.2:52654232-52654251 MS.gene00286:CDS 25.0%
! CTTCATTGTAAACATTTCTT+TGG + chr2.2:52653641-52653660 MS.gene00286:intron 25.0%
! TATAATCCCTACATTTGAAT+CGG + chr2.2:52653574-52653593 MS.gene00286:CDS 25.0%
! TCAATAACTTTCTCATTAGA+AGG - chr2.2:52653614-52653633 None:intergenic 25.0%
! TCATATCTCATTACCAATTT+CGG + chr2.2:52653275-52653294 MS.gene00286:CDS 25.0%
! TGCTTCAAATATTGATATTG+TGG + chr2.2:52653502-52653521 MS.gene00286:CDS 25.0%
!! AAGTTGTTGTCTTTAAGAAA+TGG + chr2.2:52653888-52653907 MS.gene00286:CDS 25.0%
!! ACATTTTCATTAACACCAAA+TGG - chr2.2:52654160-52654179 None:intergenic 25.0%
!! AGTTGTTGTCTTTAAGAAAT+GGG + chr2.2:52653889-52653908 MS.gene00286:CDS 25.0%
!! GTTAATATGTTACTTGATGA+TGG + chr2.2:52653686-52653705 MS.gene00286:intron 25.0%
!! TAATAAGGTGATGAGATTTT+GGG + chr2.2:52653985-52654004 MS.gene00286:CDS 25.0%
!! TTAATAAGGTGATGAGATTT+TGG + chr2.2:52653984-52654003 MS.gene00286:CDS 25.0%
AAACTATACGAAAAAACCTC+CGG - chr2.2:52654052-52654071 None:intergenic 30.0%
AAGAGTAGAAATGAACTTTG+GGG - chr2.2:52653195-52653214 None:intergenic 30.0%
AAGCTTAAGGATTTCAACAT+CGG - chr2.2:52653122-52653141 None:intergenic 30.0%
ATCCACGGAGAAAATTAATA+AGG + chr2.2:52653970-52653989 MS.gene00286:CDS 30.0%
ATTGAATTGTCTACTCCATT+TGG + chr2.2:52654142-52654161 MS.gene00286:CDS 30.0%
GAAAGAGTAGAAATGAACTT+TGG - chr2.2:52653197-52653216 None:intergenic 30.0%
TAACTTTCTCATTAGAAGGA+AGG - chr2.2:52653610-52653629 None:intergenic 30.0%
TGACAAAAATTCACATTGGT+AGG - chr2.2:52653821-52653840 None:intergenic 30.0%
TGCTTGACAAAAATTCACAT+TGG - chr2.2:52653825-52653844 None:intergenic 30.0%
TTATGATATGAGGAATGCTT+TGG + chr2.2:52653766-52653785 MS.gene00286:intron 30.0%
TTCGTATAGTTTGGAGAATA+GGG + chr2.2:52654060-52654079 MS.gene00286:CDS 30.0%
TTTCGTATAGTTTGGAGAAT+AGG + chr2.2:52654059-52654078 MS.gene00286:CDS 30.0%
! AAAGTATGTGCAGTGTTTTA+AGG + chr2.2:52654189-52654208 MS.gene00286:CDS 30.0%
! ATTCTTTTCTATCAAACCTC+TGG - chr2.2:52654122-52654141 None:intergenic 30.0%
! GTTGTTGTCTTTAAGAAATG+GGG + chr2.2:52653890-52653909 MS.gene00286:CDS 30.0%
! TTGGATTTGGTTAAGGAAAT+GGG + chr2.2:52653785-52653804 MS.gene00286:intron 30.0%
! TTTGGATTTGGTTAAGGAAA+TGG + chr2.2:52653784-52653803 MS.gene00286:intron 30.0%
!! TTCTTACCGATTCAAATGTA+GGG - chr2.2:52653583-52653602 None:intergenic 30.0%
AGACTTGAACCAAGAATTCT+AGG - chr2.2:52653545-52653564 None:intergenic 35.0%
ATTGTGTATGTTAGCATCCA+CGG + chr2.2:52653955-52653974 MS.gene00286:CDS 35.0%
CTACATTTGAATCGGTAAGA+AGG + chr2.2:52653582-52653601 MS.gene00286:intron 35.0%
GACTTGAACCAAGAATTCTA+GGG - chr2.2:52653544-52653563 None:intergenic 35.0%
GAGATATGAAACTGTGAACT+GGG - chr2.2:52653264-52653283 None:intergenic 35.0%
GGGTCACATTTAAGAATGTT+GGG - chr2.2:52653437-52653456 None:intergenic 35.0%
GTATTGTTGTGGTTCGATTT+AGG - chr2.2:52653163-52653182 None:intergenic 35.0%
TAAATGTGACCCCTACAAAA+GGG + chr2.2:52653445-52653464 MS.gene00286:CDS 35.0%
TGAGATATGAAACTGTGAAC+TGG - chr2.2:52653265-52653284 None:intergenic 35.0%
TTAAATGTGACCCCTACAAA+AGG + chr2.2:52653444-52653463 MS.gene00286:CDS 35.0%
TTCTGATGAGAAACCGAAAT+TGG - chr2.2:52653291-52653310 None:intergenic 35.0%
! AAATGGTTCTTGAAGCGTTT+AGG + chr2.2:52653924-52653943 MS.gene00286:CDS 35.0%
! CACCTTATTAATTTTCTCCG+TGG - chr2.2:52653975-52653994 None:intergenic 35.0%
! GAATGCTTTGGATTTGGTTA+AGG + chr2.2:52653778-52653797 MS.gene00286:intron 35.0%
! TATGAGGAATGCTTTGGATT+TGG + chr2.2:52653772-52653791 MS.gene00286:intron 35.0%
! TCAAGCATTGTTAGCCAAAA+GGG + chr2.2:52653838-52653857 MS.gene00286:CDS 35.0%
! TTGTCTTTAAGAAATGGGGT+TGG + chr2.2:52653894-52653913 MS.gene00286:CDS 35.0%
! TTTGGCAAAACCCTTTTGTA+GGG - chr2.2:52653458-52653477 None:intergenic 35.0%
!! AGATTTTGGGTCAATGAGTT+GGG + chr2.2:52653998-52654017 MS.gene00286:CDS 35.0%
!! ATCGCTGTTTTCAGAAACTA+CGG + chr2.2:52653371-52653390 MS.gene00286:CDS 35.0%
!! CTTCTTACCGATTCAAATGT+AGG - chr2.2:52653584-52653603 None:intergenic 35.0%
!! TCGATTTAGGTTGAGAAAGA+GGG - chr2.2:52653150-52653169 None:intergenic 35.0%
!! TTCGATTTAGGTTGAGAAAG+AGG - chr2.2:52653151-52653170 None:intergenic 35.0%
!!! GGAGGTTTTTTCGTATAGTT+TGG + chr2.2:52654051-52654070 MS.gene00286:CDS 35.0%
AAAACAGCGATTACTGAGTC+GGG - chr2.2:52653362-52653381 None:intergenic 40.0%
ACAAGAAGCCCTAGAATTCT+TGG + chr2.2:52653533-52653552 MS.gene00286:CDS 40.0%
GAAAACAGCGATTACTGAGT+CGG - chr2.2:52653363-52653382 None:intergenic 40.0%
GGGGTCACATTTAAGAATGT+TGG - chr2.2:52653438-52653457 None:intergenic 40.0%
GTGGATGCTAACATACACAA+TGG - chr2.2:52653956-52653975 None:intergenic 40.0%
! AAAGAGGGGTTTGAAGCTTA+AGG - chr2.2:52653135-52653154 None:intergenic 40.0%
! CTTTGGCAAAACCCTTTTGT+AGG - chr2.2:52653459-52653478 None:intergenic 40.0%
! GATTACTGAGTCGGGTTTTT+GGG - chr2.2:52653354-52653373 None:intergenic 40.0%
! GTCAAGCATTGTTAGCCAAA+AGG + chr2.2:52653837-52653856 MS.gene00286:CDS 40.0%
! TTCAGAGGCACAACAAATCT+TGG + chr2.2:52653090-52653109 MS.gene00286:CDS 40.0%
! TTCTTGAAGCGTTTAGGAAG+CGG + chr2.2:52653930-52653949 MS.gene00286:CDS 40.0%
! TTGGCAAAACCCTTTTGTAG+GGG - chr2.2:52653457-52653476 None:intergenic 40.0%
!! AATGAACTTTGGGGTTGGAA+AGG - chr2.2:52653186-52653205 None:intergenic 40.0%
!! ATGAACTTTGGGGTTGGAAA+GGG - chr2.2:52653185-52653204 None:intergenic 40.0%
!! CGATTTAGGTTGAGAAAGAG+GGG - chr2.2:52653149-52653168 None:intergenic 40.0%
!! GAGATTTTGGGTCAATGAGT+TGG + chr2.2:52653997-52654016 MS.gene00286:CDS 40.0%
!! GTAGAAATGAACTTTGGGGT+TGG - chr2.2:52653191-52653210 None:intergenic 40.0%
!!! GTATGTGCAGTGTTTTAAGG+AGG + chr2.2:52654192-52654211 MS.gene00286:CDS 40.0%
ACTCTTCGGCTCTTGTCAAA+AGG + chr2.2:52654026-52654045 MS.gene00286:CDS 45.0%
ATCCCAACGTGATTTCGACA+TGG - chr2.2:52653864-52653883 None:intergenic 45.0%
GGAGAATAGGGTCATTCCAA+GGG + chr2.2:52654072-52654091 MS.gene00286:CDS 45.0%
GTTTCGCATTTGATCTCCAG+AGG + chr2.2:52654103-52654122 MS.gene00286:CDS 45.0%
TGGAGAATAGGGTCATTCCA+AGG + chr2.2:52654071-52654090 MS.gene00286:CDS 45.0%
TTGTTGTGAAGTGGTTGAGG+AGG - chr2.2:52653240-52653259 None:intergenic 45.0%
! CGATTACTGAGTCGGGTTTT+TGG - chr2.2:52653355-52653374 None:intergenic 45.0%
! TGATTTCGACATGGCCCTTT+TGG - chr2.2:52653855-52653874 None:intergenic 45.0%
! TTGGGTCAATGAGTTGGGTT+GGG + chr2.2:52654003-52654022 MS.gene00286:CDS 45.0%
! TTTGGGTCAATGAGTTGGGT+TGG + chr2.2:52654002-52654021 MS.gene00286:CDS 45.0%
!! ATGGGGTTGGTCTGATGAAA+TGG + chr2.2:52653907-52653926 MS.gene00286:CDS 45.0%
!! GAACTGGGTTTGTTGTGAAG+TGG - chr2.2:52653249-52653268 None:intergenic 45.0%
!! GGTTGGAAAGGGTATTGTTG+TGG - chr2.2:52653174-52653193 None:intergenic 45.0%
!! GGTTTGTTGTGAAGTGGTTG+AGG - chr2.2:52653243-52653262 None:intergenic 45.0%
AGGAGGAGTGGTTCAAAGAG+AGG - chr2.2:52653223-52653242 None:intergenic 50.0%
GGAGGAGTGGTTCAAAGAGA+GGG - chr2.2:52653222-52653241 None:intergenic 50.0%
GGCCATGTCGAAATCACGTT+GGG + chr2.2:52653859-52653878 MS.gene00286:CDS 50.0%
TGCGAAACAACACAAGCCCT+TGG - chr2.2:52654091-52654110 None:intergenic 50.0%
TTCGGCTCTTGTCAAAAGGC+CGG + chr2.2:52654030-52654049 MS.gene00286:CDS 50.0%
!! TGAGTTGGGTTGGGACTCTT+CGG + chr2.2:52654012-52654031 MS.gene00286:CDS 50.0%
GGGCCATGTCGAAATCACGT+TGG + chr2.2:52653858-52653877 MS.gene00286:CDS 55.0%
GTGAAGTGGTTGAGGAGGAG+TGG - chr2.2:52653235-52653254 None:intergenic 55.0%
GGCTCTTGTCAAAAGGCCGG+AGG + chr2.2:52654033-52654052 MS.gene00286:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52653086 52654261 52653086 ID=MS.gene00286
chr2.2 mRNA 52653086 52654261 52653086 ID=MS.gene00286.t1;Parent=MS.gene00286
chr2.2 exon 52653086 52653595 52653086 ID=MS.gene00286.t1.exon1;Parent=MS.gene00286.t1
chr2.2 CDS 52653086 52653595 52653086 ID=cds.MS.gene00286.t1;Parent=MS.gene00286.t1
chr2.2 exon 52653800 52654261 52653800 ID=MS.gene00286.t1.exon2;Parent=MS.gene00286.t1
chr2.2 CDS 52653800 52654261 52653800 ID=cds.MS.gene00286.t1;Parent=MS.gene00286.t1
Gene Sequence

>MS.gene00286

ATGTTTCAGAGGCACAACAAATCTTGGAACTCTCCGATGTTGAAATCCTTAAGCTTCAAACCCCTCTTTCTCAACCTAAATCGAACCACAACAATACCCTTTCCAACCCCAAAGTTCATTTCTACTCTTTCAACCCTCTCTTTGAACCACTCCTCCTCAACCACTTCACAACAAACCCAGTTCACAGTTTCATATCTCATTACCAATTTCGGTTTCTCATCAGAAACAGCTTCAAAAGCTTCAAAACTAGTTCAATTCGACACTTCCCAAAAACCCGACTCAGTAATCGCTGTTTTCAGAAACTACGGTTTCTCTGATTCTCAGATAAAAAACATCATTAGACAAGCACCCAACATTCTTAAATGTGACCCCTACAAAAGGGTTTTGCCAAAGTTTGAATTTTTAACTTCAAAAGGTGCTTCAAATATTGATATTGTGGAAATAGTTACAAGAAGCCCTAGAATTCTTGGTTCAAGTCTTGAGAATAGTATAATCCCTACATTTGAATCGGTAAGAAGGTTCCTTCCTTCTAATGAGAAAGTTATTGAACGTATACTTCATTGTAAACATTTCTTTGGTCATTTTCATTTTATTAGAAATGTTAATATGTTACTTGATGATGGAGTCATTCACTCAAATATCACTTTTTTATTGCTTAGAAGACCGTCTATATTATTGACTTATGATATGAGGAATGCTTTGGATTTGGTTAAGGAAATGGGTTTTGATGATCCTACCAATGTGAATTTTTGTCAAGCATTGTTAGCCAAAAGGGCCATGTCGAAATCACGTTGGGATGCGAAAGTTGTTGTCTTTAAGAAATGGGGTTGGTCTGATGAAATGGTTCTTGAAGCGTTTAGGAAGCGGCCATTGTGTATGTTAGCATCCACGGAGAAAATTAATAAGGTGATGAGATTTTGGGTCAATGAGTTGGGTTGGGACTCTTCGGCTCTTGTCAAAAGGCCGGAGGTTTTTTCGTATAGTTTGGAGAATAGGGTCATTCCAAGGGCTTGTGTTGTTTCGCATTTGATCTCCAGAGGTTTGATAGAAAAGAATATTGAATTGTCTACTCCATTTGGTGTTAATGAAAATGTGTTTCTTGAAAAGTATGTGCAGTGTTTTAAGGAGGAAAGAGATGATTTGTTAAAGCTATACCTAGAAAAAATGGGTGTCTGA
Protein sequence

>MS.gene00286.t1

MFQRHNKSWNSPMLKSLSFKPLFLNLNRTTTIPFPTPKFISTLSTLSLNHSSSTTSQQTQFTVSYLITNFGFSSETASKASKLVQFDTSQKPDSVIAVFRNYGFSDSQIKNIIRQAPNILKCDPYKRVLPKFEFLTSKGASNIDIVEIVTRSPRILGSSLENSIIPTFESEMGFDDPTNVNFCQALLAKRAMSKSRWDAKVVVFKKWGWSDEMVLEAFRKRPLCMLASTEKINKVMRFWVNELGWDSSALVKRPEVFSYSLENRVIPRACVVSHLISRGLIEKNIELSTPFGVNENVFLEKYVQCFKEERDDLLKLYLEKMGV