AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004585

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004585.t1 MTR_2g437080 94.764 382 20 0 1 382 1 382 0.0 741
MS.gene004585.t1 MTR_2g437060 74.093 386 88 2 6 379 9 394 0.0 565
MS.gene004585.t1 MTR_2g437030 66.923 390 119 2 1 381 79 467 0.0 515
MS.gene004585.t1 MTR_2g436400 68.548 372 115 1 5 374 10 381 1.61e-180 506
MS.gene004585.t1 MTR_2g437020 65.714 385 120 4 3 382 5 382 2.12e-179 504
MS.gene004585.t1 MTR_2g436440 66.205 361 122 0 19 379 25 385 1.16e-174 492
MS.gene004585.t1 MTR_2g437040 62.797 379 141 0 1 379 1 379 1.92e-167 473
MS.gene004585.t1 MTR_2g437160 53.315 362 167 1 21 380 44 405 4.05e-130 379
MS.gene004585.t1 MTR_2g437120 53.107 354 161 3 27 376 37 389 3.04e-127 372
MS.gene004585.t1 MTR_2g437200 48.872 399 161 5 13 370 2 398 1.76e-124 365
MS.gene004585.t1 MTR_2g437960 52.959 338 156 1 42 376 55 392 4.65e-124 364
MS.gene004585.t1 MTR_2g437170 52.235 358 162 3 32 380 43 400 7.03e-124 363
MS.gene004585.t1 MTR_2g437960 52.959 338 156 1 42 376 55 392 1.42e-123 363
MS.gene004585.t1 MTR_2g437150 51.507 365 167 4 26 380 36 400 9.89e-123 360
MS.gene004585.t1 MTR_2g437990 51.657 362 166 3 27 380 32 392 2.57e-122 360
MS.gene004585.t1 MTR_2g437180 52.089 359 164 5 26 380 36 390 5.98e-121 355
MS.gene004585.t1 MTR_2g437260 48.732 355 176 3 24 374 18 370 1.92e-119 352
MS.gene004585.t1 MTR_4g119550 51.304 345 165 2 36 379 47 389 2.66e-119 351
MS.gene004585.t1 MTR_2g076320 49.862 363 172 3 27 380 32 393 4.84e-119 351
MS.gene004585.t1 MTR_2g437130 48.925 372 184 3 5 376 12 377 1.05e-118 350
MS.gene004585.t1 MTR_2g438010 49.465 374 171 4 24 380 41 413 3.75e-118 349
MS.gene004585.t1 MTR_2g437240 47.188 409 171 6 14 380 3 408 1.12e-116 345
MS.gene004585.t1 MTR_2g438020 48.413 378 155 3 39 378 70 445 4.35e-116 347
MS.gene004585.t1 MTR_2g437100 51.344 372 160 5 26 383 37 401 3.71e-115 341
MS.gene004585.t1 MTR_2g436460 45.093 377 191 3 11 373 5 379 4.45e-107 320
MS.gene004585.t1 MTR_2g437940 48.169 355 150 4 26 376 36 360 2.37e-103 310
MS.gene004585.t1 MTR_4g119580 44.606 343 186 2 30 370 32 372 8.35e-96 291
MS.gene004585.t1 MTR_2g019840 45.767 378 190 9 1 370 1 371 2.74e-92 282
MS.gene004585.t1 MTR_4g120380 44.611 334 183 1 34 367 38 369 1.27e-91 280
MS.gene004585.t1 MTR_2g019810 46.988 332 170 4 41 370 32 359 4.47e-91 278
MS.gene004585.t1 MTR_4g120380 44.762 315 172 1 34 348 38 350 1.07e-85 266
MS.gene004585.t1 MTR_2g437090 62.941 170 63 0 213 382 1 170 3.17e-77 236
MS.gene004585.t1 MTR_2g437160 48.571 245 109 2 153 380 70 314 2.61e-68 218
MS.gene004585.t1 MTR_2g436370 59.358 187 74 1 5 189 9 195 7.32e-67 211
MS.gene004585.t1 MTR_3g450500 31.977 344 210 5 41 370 39 372 1.05e-51 177
MS.gene004585.t1 MTR_4g119570 38.462 247 119 1 120 366 14 227 1.29e-49 167
MS.gene004585.t1 MTR_4g007490 29.255 376 236 7 5 368 11 368 6.49e-44 156
MS.gene004585.t1 MTR_8g080990 29.641 334 229 4 35 364 37 368 1.41e-34 131
MS.gene004585.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 4.53e-29 116
MS.gene004585.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 4.72e-29 117
MS.gene004585.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 4.74e-29 117
MS.gene004585.t1 MTR_4g049400 45.455 121 61 2 26 141 31 151 7.32e-25 101
MS.gene004585.t1 MTR_2g436380 72.131 61 17 0 213 273 1 61 9.84e-24 100
MS.gene004585.t1 MTR_3g085240 37.762 143 79 3 21 163 39 171 1.71e-20 90.1
MS.gene004585.t1 MTR_3g085240 64.151 53 19 0 272 324 176 228 3.51e-15 75.1
MS.gene004585.t1 MTR_8g012210 21.854 302 201 7 86 368 63 348 1.23e-12 68.9
MS.gene004585.t1 MTR_4g119615 52.000 50 24 0 312 361 26 75 8.99e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004585.t1 AT5G07900 34.328 335 208 6 41 367 69 399 2.06e-63 208
MS.gene004585.t1 AT1G21150 30.792 341 228 4 31 365 10 348 1.09e-52 179
MS.gene004585.t1 AT1G21150 31.722 331 218 4 41 365 56 384 1.57e-52 179
MS.gene004585.t1 AT1G21150 31.722 331 218 4 41 365 120 448 9.71e-52 179
MS.gene004585.t1 AT1G61980 28.378 370 216 9 41 367 52 415 3.88e-34 131
MS.gene004585.t1 AT1G61980 28.378 370 216 9 41 367 52 415 3.88e-34 131
MS.gene004585.t1 AT1G61980 28.378 370 216 9 41 367 52 415 3.88e-34 131
MS.gene004585.t1 AT5G64950 27.731 357 233 8 31 368 26 376 6.40e-32 124
MS.gene004585.t1 AT1G61990 27.078 421 236 11 2 367 4 408 6.45e-32 125
MS.gene004585.t1 AT1G61990 27.078 421 236 11 2 367 4 408 6.45e-32 125
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G61970 27.066 351 213 7 42 353 53 399 8.27e-32 124
MS.gene004585.t1 AT1G62085 27.053 414 213 13 2 329 4 414 1.16e-29 119
MS.gene004585.t1 AT1G62085 27.053 414 213 13 2 329 4 414 1.16e-29 119
MS.gene004585.t1 AT3G46950 29.592 294 191 7 2 285 4 291 2.54e-29 118
MS.gene004585.t1 AT1G62120 27.217 327 198 5 42 330 57 381 9.00e-29 116
MS.gene004585.t1 AT5G23930 26.010 396 211 9 41 363 55 441 1.23e-26 110
MS.gene004585.t1 AT1G62110 28.571 245 170 3 42 283 53 295 3.00e-24 103
MS.gene004585.t1 AT1G62010 26.578 301 174 6 71 330 65 359 1.84e-23 101
MS.gene004585.t1 AT1G62150 23.035 369 209 7 35 330 52 418 7.66e-23 100
MS.gene004585.t1 AT1G61960 27.797 295 195 7 3 283 4 294 1.93e-21 95.9
MS.gene004585.t1 AT1G79220 24.183 306 193 8 79 380 119 389 1.57e-11 65.9

Find 79 sgRNAs with CRISPR-Local

Find 99 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTTATCAACAATTT+TGG 0.080401 2.2:-52159357 MS.gene004585:CDS
TGAAGGTGGTGGATGAATTA+AGG 0.202555 2.2:-52158849 MS.gene004585:CDS
AAAGGGTTTGAGATTGAAAA+TGG 0.228978 2.2:+52159464 None:intergenic
CGACAAAATATCTGGTGTTT+TGG 0.229116 2.2:+52158588 None:intergenic
CGACCAACCCCACTTCTTAA+AGG 0.236453 2.2:+52158732 None:intergenic
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2.2:-52158645 MS.gene004585:CDS
GAAAATGGTAATGGGGTTAT+TGG 0.243519 2.2:+52159426 None:intergenic
TTTCCTGCGACCCCTCTAAA+AGG 0.248958 2.2:-52159182 MS.gene004585:CDS
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2.2:-52158526 MS.gene004585:CDS
GTGGATGAATTAAGGGATTT+GGG 0.278480 2.2:-52158841 MS.gene004585:CDS
GGTGGATGAATTAAGGGATT+TGG 0.320429 2.2:-52158842 MS.gene004585:CDS
TTTCTCTTTCCACAAGGCTT+TGG 0.324915 2.2:+52158762 None:intergenic
TGAGATTGAAAATGGATCTA+TGG 0.333993 2.2:+52159472 None:intergenic
CGAGAACAGAATCAGGGTTT+TGG 0.341656 2.2:+52159274 None:intergenic
AATGAAGAGAAAATGGTAAT+GGG 0.355617 2.2:+52159418 None:intergenic
TATCGTTCCAATCTATGAAT+TGG 0.356154 2.2:-52159052 MS.gene004585:CDS
GTGCAGAAATGAAGAGAAAA+TGG 0.357192 2.2:+52159411 None:intergenic
CAAATATTTCTAGATTGCTT+AGG 0.360863 2.2:-52158906 MS.gene004585:CDS
GAAGGTGGTGGATGAATTAA+GGG 0.364635 2.2:-52158848 MS.gene004585:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.2:-52158626 MS.gene004585:CDS
AAGAAATCGAGAACAGAATC+AGG 0.368524 2.2:+52159267 None:intergenic
AAACTTAACGACAAAATATC+TGG 0.378028 2.2:+52158580 None:intergenic
AAAATGGTAATGGGGTTATT+GGG 0.394325 2.2:+52159427 None:intergenic
GGGTATGGTGAGAAAGTAAA+GGG 0.406633 2.2:+52159447 None:intergenic
CTATAAACCAATTCATAGAT+TGG 0.415060 2.2:+52159045 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.2:+52159131 None:intergenic
CAAACCCAAAATTGTTGATA+AGG 0.421423 2.2:+52159352 None:intergenic
GAAAGTAGCCACGGTGCCTT+GGG 0.421775 2.2:+52159201 None:intergenic
GGAAAGTAGCCACGGTGCCT+TGG 0.424368 2.2:+52159200 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.2:-52158627 MS.gene004585:CDS
AAATATTTCTAGATTGCTTA+GGG 0.430507 2.2:-52158905 MS.gene004585:CDS
ATTACATTGTTGCTTCAGAA+TGG 0.447044 2.2:-52158940 MS.gene004585:CDS
AATTGCACAACAGTGGCTCT+TGG 0.457636 2.2:+52158541 None:intergenic
GGGAATGATACGACGTAGTT+GGG 0.467688 2.2:+52159221 None:intergenic
AGATGGTCACTAAATAAAAC+AGG 0.477887 2.2:+52158976 None:intergenic
AAATGAAGAGAAAATGGTAA+TGG 0.484200 2.2:+52159417 None:intergenic
TGGGAATGATACGACGTAGT+TGG 0.489187 2.2:+52159220 None:intergenic
AACTTCTCAGGCATTAAGAA+TGG 0.491818 2.2:+52158472 None:intergenic
CTCGATTTCTTTAGAAACCA+TGG 0.495225 2.2:-52159255 MS.gene004585:CDS
GTATCAAGAAATAACTTCTC+AGG 0.495468 2.2:+52158460 None:intergenic
TGGGTATGGTGAGAAAGTAA+AGG 0.497840 2.2:+52159446 None:intergenic
GGTAATGGGGTTATTGGGTA+TGG 0.511895 2.2:+52159432 None:intergenic
TTTATTTAGTGACCATCTTG+TGG 0.528406 2.2:-52158971 MS.gene004585:CDS
CAAAGCTTAGAAGCAAATTG+TGG 0.530033 2.2:+52159327 None:intergenic
TCAGTTGGGTTGGGATGCTA+AGG 0.533835 2.2:-52158617 MS.gene004585:CDS
CAAGCTTACAGACAAGATCC+TGG 0.534656 2.2:+52159083 None:intergenic
AAGCTTACAGACAAGATCCT+GGG 0.540938 2.2:+52159084 None:intergenic
AGTTGATGCCTTTAAGAAGT+GGG 0.541443 2.2:-52158740 MS.gene004585:CDS
TTCCTGCGACCCCTCTAAAA+GGG 0.542437 2.2:-52159181 MS.gene004585:CDS
CTTGAAACGGAGCTTGTAAG+TGG 0.546088 2.2:+52159302 None:intergenic
CAACTTTAAAAGATTAGAAG+CGG 0.547155 2.2:+52158414 None:intergenic
AAGTTGATGCCTTTAAGAAG+TGG 0.551383 2.2:-52158741 MS.gene004585:CDS
AAAGCTTAGAAGCAAATTGT+GGG 0.556435 2.2:+52159328 None:intergenic
CTTTAAGAAGTGGGGTTGGT+CGG 0.561255 2.2:-52158731 MS.gene004585:CDS
ACTTTATGAGTAAGAACCCC+AGG 0.563877 2.2:-52159101 MS.gene004585:CDS
ATGCCTTTAAGAAGTGGGGT+TGG 0.572339 2.2:-52158735 MS.gene004585:CDS
TAGATTCTTGCAATCGCACA+AGG 0.582615 2.2:-52159025 MS.gene004585:CDS
TGTTCGTGGTGTATGCAAGA+TGG 0.584185 2.2:+52158377 None:intergenic
CATCAACAACCAAAGCCTTG+TGG 0.591454 2.2:-52158771 MS.gene004585:CDS
CAATCTAGAAATATTTGAGT+CGG 0.591851 2.2:+52158912 None:intergenic
AAGGTATGAAACTGCAAATG+AGG 0.595055 2.2:+52159371 None:intergenic
ATGAAGAGAAAATGGTAATG+GGG 0.595101 2.2:+52159419 None:intergenic
ATCAACTTTCTCTTTCCACA+AGG 0.604525 2.2:+52158756 None:intergenic
TGGATGAATTAAGGGATTTG+GGG 0.606794 2.2:-52158840 MS.gene004585:CDS
TAATGTTATGTGCCACAAGA+TGG 0.610046 2.2:+52158959 None:intergenic
GTTGATGCCTTTAAGAAGTG+GGG 0.614341 2.2:-52158739 MS.gene004585:CDS
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2.2:+52159238 None:intergenic
AGAAATCGAGAACAGAATCA+GGG 0.621735 2.2:+52159268 None:intergenic
GGGTCGCAGGAAAGTAGCCA+CGG 0.625027 2.2:+52159192 None:intergenic
TGAGGTTGAATCTGAAGTGT+TGG 0.626308 2.2:+52159389 None:intergenic
CATAAAGTTAACCATGTCAG+AGG 0.632724 2.2:+52159116 None:intergenic
AGGTGCTTCTACCTCTGACA+TGG 0.645076 2.2:-52159127 MS.gene004585:CDS
GAGAAGAAATTGCACAACAG+TGG 0.646434 2.2:+52158534 None:intergenic
CAAATGTGTGCAATGTCCTA+CGG 0.648773 2.2:+52158878 None:intergenic
ATGGATGTTAACATACAATG+AGG 0.655070 2.2:+52158676 None:intergenic
ACTACGTCGTATCATTCCCA+AGG 0.661929 2.2:-52159217 MS.gene004585:CDS
AGCTTACAGACAAGATCCTG+GGG 0.666533 2.2:+52159085 None:intergenic
GATTGCTTAGGGAACACCGT+AGG 0.718101 2.2:-52158894 MS.gene004585:CDS
GTATCATTCCCAAGGCACCG+TGG 0.787154 2.2:-52159209 MS.gene004585:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG + chr2.2:52159195-52159214 None:intergenic 15.0%
!!! AAATATTTCTAGATTGCTTA+GGG - chr2.2:52158944-52158963 MS.gene004585:CDS 20.0%
! AAACTTAACGACAAAATATC+TGG + chr2.2:52159272-52159291 None:intergenic 25.0%
! AAATGAAGAGAAAATGGTAA+TGG + chr2.2:52158435-52158454 None:intergenic 25.0%
! AATGAAGAGAAAATGGTAAT+GGG + chr2.2:52158434-52158453 None:intergenic 25.0%
! CAATCTAGAAATATTTGAGT+CGG + chr2.2:52158940-52158959 None:intergenic 25.0%
! CTATAAACCAATTCATAGAT+TGG + chr2.2:52158807-52158826 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.2:52158699-52158718 None:intergenic 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.2:52159035-52159054 MS.gene004585:CDS 25.0%
!! AGATATTTTGTCGTTAAGTT+TGG - chr2.2:52159271-52159290 MS.gene004585:CDS 25.0%
!! CAACTTTAAAAGATTAGAAG+CGG + chr2.2:52159438-52159457 None:intergenic 25.0%
!! CATACCTTATCAACAATTTT+GGG - chr2.2:52158493-52158512 MS.gene004585:CDS 25.0%
!! TAATTTAGTGATGAGCTTTT+GGG - chr2.2:52159205-52159224 MS.gene004585:CDS 25.0%
!! TCATACCTTATCAACAATTT+TGG - chr2.2:52158492-52158511 MS.gene004585:CDS 25.0%
!! TTAATTTAGTGATGAGCTTT+TGG - chr2.2:52159204-52159223 MS.gene004585:CDS 25.0%
!!! CAAATATTTCTAGATTGCTT+AGG - chr2.2:52158943-52158962 MS.gene004585:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.2:52158702-52158721 MS.gene004585:CDS 25.0%
AAAGCTTAGAAGCAAATTGT+GGG + chr2.2:52158524-52158543 None:intergenic 30.0%
AGATGGTCACTAAATAAAAC+AGG + chr2.2:52158876-52158895 None:intergenic 30.0%
ATGGATGTTAACATACAATG+AGG + chr2.2:52159176-52159195 None:intergenic 30.0%
CAAACCCAAAATTGTTGATA+AGG + chr2.2:52158500-52158519 None:intergenic 30.0%
GTATCAAGAAATAACTTCTC+AGG + chr2.2:52159392-52159411 None:intergenic 30.0%
GTGCAATTTCTTCTCAATAA+TGG - chr2.2:52159323-52159342 MS.gene004585:CDS 30.0%
TATCGTTCCAATCTATGAAT+TGG - chr2.2:52158797-52158816 MS.gene004585:CDS 30.0%
TGAGATTGAAAATGGATCTA+TGG + chr2.2:52158380-52158399 None:intergenic 30.0%
TGTCGTTAAGTTTGGAAAAA+AGG - chr2.2:52159279-52159298 MS.gene004585:CDS 30.0%
TTTATTTAGTGACCATCTTG+TGG - chr2.2:52158878-52158897 MS.gene004585:CDS 30.0%
! AAAGGGTTTGAGATTGAAAA+TGG + chr2.2:52158388-52158407 None:intergenic 30.0%
! ATGAAGAGAAAATGGTAATG+GGG + chr2.2:52158433-52158452 None:intergenic 30.0%
! ATTACATTGTTGCTTCAGAA+TGG - chr2.2:52158909-52158928 MS.gene004585:CDS 30.0%
!! AAAATGGTAATGGGGTTATT+GGG + chr2.2:52158425-52158444 None:intergenic 30.0%
!!! ACACCAAAAGTTGTTTTTGA+AGG + chr2.2:52159041-52159060 None:intergenic 30.0%
AACTTCTCAGGCATTAAGAA+TGG + chr2.2:52159380-52159399 None:intergenic 35.0%
AAGAAATCGAGAACAGAATC+AGG + chr2.2:52158585-52158604 None:intergenic 35.0%
AAGGTATGAAACTGCAAATG+AGG + chr2.2:52158481-52158500 None:intergenic 35.0%
AGAAATCGAGAACAGAATCA+GGG + chr2.2:52158584-52158603 None:intergenic 35.0%
ATCAACTTTCTCTTTCCACA+AGG + chr2.2:52159096-52159115 None:intergenic 35.0%
CAAAGCTTAGAAGCAAATTG+TGG + chr2.2:52158525-52158544 None:intergenic 35.0%
CATAAAGTTAACCATGTCAG+AGG + chr2.2:52158736-52158755 None:intergenic 35.0%
GTGCAGAAATGAAGAGAAAA+TGG + chr2.2:52158441-52158460 None:intergenic 35.0%
GTGGATGAATTAAGGGATTT+GGG - chr2.2:52159008-52159027 MS.gene004585:CDS 35.0%
TAATGTTATGTGCCACAAGA+TGG + chr2.2:52158893-52158912 None:intergenic 35.0%
TGGATGAATTAAGGGATTTG+GGG - chr2.2:52159009-52159028 MS.gene004585:CDS 35.0%
! AGTTGATGCCTTTAAGAAGT+GGG - chr2.2:52159109-52159128 MS.gene004585:CDS 35.0%
! CGACAAAATATCTGGTGTTT+TGG + chr2.2:52159264-52159283 None:intergenic 35.0%
! CTCGATTTCTTTAGAAACCA+TGG - chr2.2:52158594-52158613 MS.gene004585:CDS 35.0%
! GCACACATTTGACATTTTGA+AGG - chr2.2:52158983-52159002 MS.gene004585:CDS 35.0%
! TTTCGTGCAATTTTTGTTCG+TGG + chr2.2:52159489-52159508 None:intergenic 35.0%
!! AAGTTGATGCCTTTAAGAAG+TGG - chr2.2:52159108-52159127 MS.gene004585:CDS 35.0%
!! ATTTGACATTTTGAAGGTGG+TGG - chr2.2:52158989-52159008 MS.gene004585:CDS 35.0%
!! CACATTTGACATTTTGAAGG+TGG - chr2.2:52158986-52159005 MS.gene004585:CDS 35.0%
!! GAAAATGGTAATGGGGTTAT+TGG + chr2.2:52158426-52158445 None:intergenic 35.0%
AAGCTTACAGACAAGATCCT+GGG + chr2.2:52158768-52158787 None:intergenic 40.0%
ACTTTATGAGTAAGAACCCC+AGG - chr2.2:52158748-52158767 MS.gene004585:CDS 40.0%
CAAATGTGTGCAATGTCCTA+CGG + chr2.2:52158974-52158993 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+TGG + chr2.2:52159318-52159337 None:intergenic 40.0%
GGGTATGGTGAGAAAGTAAA+GGG + chr2.2:52158405-52158424 None:intergenic 40.0%
GGTGGATGAATTAAGGGATT+TGG - chr2.2:52159007-52159026 MS.gene004585:CDS 40.0%
TAGATTCTTGCAATCGCACA+AGG - chr2.2:52158824-52158843 MS.gene004585:CDS 40.0%
TGGGTATGGTGAGAAAGTAA+AGG + chr2.2:52158406-52158425 None:intergenic 40.0%
TTTCTCTTTCCACAAGGCTT+TGG + chr2.2:52159090-52159109 None:intergenic 40.0%
! GTTGATGCCTTTAAGAAGTG+GGG - chr2.2:52159110-52159129 MS.gene004585:CDS 40.0%
! TGAGGTTGAATCTGAAGTGT+TGG + chr2.2:52158463-52158482 None:intergenic 40.0%
! TTTGGCAACACCCTTTTAGA+GGG + chr2.2:52158681-52158700 None:intergenic 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.2:52159218-52159237 MS.gene004585:CDS 40.0%
!! GAAGGTGGTGGATGAATTAA+GGG - chr2.2:52159001-52159020 MS.gene004585:CDS 40.0%
!! GGTTTTGGTCAGTCTTGAAA+CGG + chr2.2:52158563-52158582 None:intergenic 40.0%
!! TGAAGGTGGTGGATGAATTA+AGG - chr2.2:52159000-52159019 MS.gene004585:CDS 40.0%
AATTGCACAACAGTGGCTCT+TGG + chr2.2:52159311-52159330 None:intergenic 45.0%
ACTACGTCGTATCATTCCCA+AGG - chr2.2:52158632-52158651 MS.gene004585:CDS 45.0%
AGCTTACAGACAAGATCCTG+GGG + chr2.2:52158767-52158786 None:intergenic 45.0%
CAAGCTTACAGACAAGATCC+TGG + chr2.2:52158769-52158788 None:intergenic 45.0%
CATCAACAACCAAAGCCTTG+TGG - chr2.2:52159078-52159097 MS.gene004585:CDS 45.0%
CTTGAAACGGAGCTTGTAAG+TGG + chr2.2:52158550-52158569 None:intergenic 45.0%
GGGAATGATACGACGTAGTT+GGG + chr2.2:52158631-52158650 None:intergenic 45.0%
GTTGGGAGTTAGAGAAACCA+TGG + chr2.2:52158614-52158633 None:intergenic 45.0%
TGGGAATGATACGACGTAGT+TGG + chr2.2:52158632-52158651 None:intergenic 45.0%
TGTTCGTGGTGTATGCAAGA+TGG + chr2.2:52159475-52159494 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.2:52159223-52159242 MS.gene004585:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.2:52159222-52159241 MS.gene004585:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG - chr2.2:52159114-52159133 MS.gene004585:CDS 45.0%
! CGAGAACAGAATCAGGGTTT+TGG + chr2.2:52158578-52158597 None:intergenic 45.0%
! CTTTAAGAAGTGGGGTTGGT+CGG - chr2.2:52159118-52159137 MS.gene004585:CDS 45.0%
! CTTTGGCAACACCCTTTTAG+AGG + chr2.2:52158682-52158701 None:intergenic 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.2:52159217-52159236 MS.gene004585:CDS 45.0%
!! GGTAATGGGGTTATTGGGTA+TGG + chr2.2:52158420-52158439 None:intergenic 45.0%
!! TTGGCAACACCCTTTTAGAG+GGG + chr2.2:52158680-52158699 None:intergenic 45.0%
AGGTGCTTCTACCTCTGACA+TGG - chr2.2:52158722-52158741 MS.gene004585:CDS 50.0%
CGACCAACCCCACTTCTTAA+AGG + chr2.2:52159120-52159139 None:intergenic 50.0%
GATTGCTTAGGGAACACCGT+AGG - chr2.2:52158955-52158974 MS.gene004585:CDS 50.0%
TTTCCTGCGACCCCTCTAAA+AGG - chr2.2:52158667-52158686 MS.gene004585:CDS 50.0%
! TTCCTGCGACCCCTCTAAAA+GGG - chr2.2:52158668-52158687 MS.gene004585:CDS 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.2:52158721-52158740 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+AGG - chr2.2:52159232-52159251 MS.gene004585:CDS 50.0%
GAAAGTAGCCACGGTGCCTT+GGG + chr2.2:52158651-52158670 None:intergenic 55.0%
!! GTATCATTCCCAAGGCACCG+TGG - chr2.2:52158640-52158659 MS.gene004585:CDS 55.0%
GGAAAGTAGCCACGGTGCCT+TGG + chr2.2:52158652-52158671 None:intergenic 60.0%
GGGTCGCAGGAAAGTAGCCA+CGG + chr2.2:52158660-52158679 None:intergenic 60.0%
!! CACCCTTTTAGAGGGGTCGC+AGG + chr2.2:52158673-52158692 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52158360 52159511 52158360 ID=MS.gene004585
chr2.2 mRNA 52158360 52159511 52158360 ID=MS.gene004585.t1;Parent=MS.gene004585
chr2.2 exon 52158360 52159511 52158360 ID=MS.gene004585.t1.exon1;Parent=MS.gene004585.t1
chr2.2 CDS 52158360 52159511 52158360 ID=cds.MS.gene004585.t1;Parent=MS.gene004585.t1
Gene Sequence

>MS.gene004585

ATGTTGATTATTCATCGCCATAGATCCATTTTCAATCTCAAACCCTTTACTTTCTCACCATACCCAATAACCCCATTACCATTTTCTCTTCATTTCTGCACCAACACTTCAGATTCAACCTCATTTGCAGTTTCATACCTTATCAACAATTTTGGGTTTGACCCACAATTTGCTTCTAAGCTTTGCTCCACTTACAAGCTCCGTTTCAAGACTGACCAAAACCCTGATTCTGTTCTCGATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCGTGGCTACTTTCCTGCGACCCCTCTAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATGGTTAACTTTATGAGTAAGAACCCCAGGATCTTGTCTGTAAGCTTGAAGAATCGTATCGTTCCAATCTATGAATTGGTTTATAGATTCTTGCAATCGCACAAGGACGCTATTGCTTGTGTAATTAGCAATCCTGTTTTATTTAGTGACCATCTTGTGGCACATAACATTACATTGTTGCTTCAGAATGGACTCTCCGACTCAAATATTTCTAGATTGCTTAGGGAACACCGTAGGACATTGCACACATTTGACATTTTGAAGGTGGTGGATGAATTAAGGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCTTTGATAGCGAAATCATCAACAACCAAAGCCTTGTGGAAAGAGAAAGTTGATGCCTTTAAGAAGTGGGGTTGGTCGGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTAAGGACATTGCCAAAACACCAGATATTTTGTCGTTAAGTTTGGAAAAAAGGATCATTCCAAGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAATAAGAATTCAAGCTTAATTTATCCATTCTTAATGCCTGAGAAGTTATTTCTTGATACGTTTGTAAAACGTTTTGAGAATGCCGCTTCTAATCTTTTAAAGTTGTATGAAGAAAAACTCCATCTTGCATACACCACGAACAAAAATTGCACGAAATGA
Protein sequence

>MS.gene004585.t1

MLIIHRHRSIFNLKPFTFSPYPITPLPFSLHFCTNTSDSTSFAVSYLINNFGFDPQFASKLCSTYKLRFKTDQNPDSVLDFFRNHGFSNSQLRRIIPKAPWLLSCDPSKRVLPKFQFFLSKGASTSDMVNFMSKNPRILSVSLKNRIVPIYELVYRFLQSHKDAIACVISNPVLFSDHLVAHNITLLLQNGLSDSNISRLLREHRRTLHTFDILKVVDELRDLGFNPSKTTFGVALIAKSSTTKALWKEKVDAFKKWGWSDEDVLEAFKKQPHCMLTSIDKINLVMSFWVNQLGWDAKDIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRNKNSSLIYPFLMPEKLFLDTFVKRFENAASNLLKLYEEKLHLAYTTNKNCTK