AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004581

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004581.t1 MTR_2g437020 93.995 383 23 0 1 383 1 383 0.0 733
MS.gene004581.t1 MTR_2g437060 69.018 397 104 5 1 380 1 395 0.0 534
MS.gene004581.t1 MTR_2g437080 66.494 385 117 4 5 382 3 382 0.0 511
MS.gene004581.t1 MTR_2g436440 69.189 370 110 3 15 383 23 389 4.13e-178 501
MS.gene004581.t1 MTR_2g436400 64.583 384 110 4 7 374 8 381 1.64e-172 486
MS.gene004581.t1 MTR_2g437030 68.108 370 114 3 16 383 102 469 1.96e-172 489
MS.gene004581.t1 MTR_2g437040 67.227 357 114 2 25 380 26 380 5.38e-167 472
MS.gene004581.t1 MTR_2g437120 57.065 368 149 7 17 378 27 391 2.17e-133 388
MS.gene004581.t1 MTR_2g437150 54.278 374 157 7 17 378 27 398 9.00e-129 376
MS.gene004581.t1 MTR_2g437170 54.545 374 156 7 17 378 27 398 1.80e-127 373
MS.gene004581.t1 MTR_2g437960 53.100 371 163 4 19 380 28 396 6.46e-126 369
MS.gene004581.t1 MTR_2g437960 52.957 372 164 4 19 381 28 397 9.88e-126 369
MS.gene004581.t1 MTR_4g119550 54.179 347 153 4 35 379 47 389 1.34e-124 365
MS.gene004581.t1 MTR_2g438010 52.066 363 161 5 22 374 48 407 1.07e-122 361
MS.gene004581.t1 MTR_2g437160 52.304 369 166 5 17 378 38 403 7.73e-122 358
MS.gene004581.t1 MTR_2g437200 50.390 385 146 6 27 370 18 398 1.21e-121 357
MS.gene004581.t1 MTR_2g437180 51.058 378 176 6 5 378 16 388 1.91e-121 357
MS.gene004581.t1 MTR_2g437260 50.000 354 172 3 24 374 19 370 1.52e-120 354
MS.gene004581.t1 MTR_2g437990 51.639 366 165 6 22 378 28 390 1.78e-120 355
MS.gene004581.t1 MTR_2g076320 52.316 367 162 5 22 378 28 391 2.49e-120 354
MS.gene004581.t1 MTR_2g437100 54.054 370 152 7 17 370 28 395 1.45e-119 353
MS.gene004581.t1 MTR_2g437130 51.064 376 173 6 7 378 11 379 6.38e-118 348
MS.gene004581.t1 MTR_2g437240 48.201 417 166 8 1 378 1 406 5.70e-115 341
MS.gene004581.t1 MTR_2g436460 49.422 346 169 4 30 373 38 379 2.70e-108 323
MS.gene004581.t1 MTR_2g437940 49.728 368 147 7 17 378 27 362 2.48e-107 320
MS.gene004581.t1 MTR_2g438020 47.619 378 155 5 38 376 70 443 4.01e-107 324
MS.gene004581.t1 MTR_4g119580 45.773 343 179 4 29 368 32 370 2.76e-94 287
MS.gene004581.t1 MTR_2g019840 46.234 385 184 8 9 379 5 380 1.87e-92 283
MS.gene004581.t1 MTR_4g120380 45.994 337 177 3 33 368 38 370 1.24e-89 275
MS.gene004581.t1 MTR_4g120380 47.003 317 164 2 33 349 38 350 6.20e-87 270
MS.gene004581.t1 MTR_2g019810 47.164 335 166 7 40 370 32 359 1.28e-86 267
MS.gene004581.t1 MTR_2g437090 70.000 170 50 1 214 382 1 170 9.64e-84 253
MS.gene004581.t1 MTR_2g436370 57.921 202 62 2 1 187 1 194 6.36e-67 211
MS.gene004581.t1 MTR_2g437160 54.950 202 85 3 181 378 113 312 2.65e-66 213
MS.gene004581.t1 MTR_2g437160 45.614 114 56 3 17 125 38 150 9.54e-19 86.7
MS.gene004581.t1 MTR_3g450500 33.241 361 222 4 19 364 9 365 1.52e-52 179
MS.gene004581.t1 MTR_4g007490 32.530 332 209 7 27 350 25 349 3.17e-43 154
MS.gene004581.t1 MTR_4g119570 37.349 249 120 3 119 366 14 227 3.23e-43 150
MS.gene004581.t1 MTR_8g080990 29.630 297 202 2 72 364 74 367 3.45e-31 122
MS.gene004581.t1 MTR_8g081000 25.656 343 246 3 34 375 50 384 7.21e-28 113
MS.gene004581.t1 MTR_8g081000 25.656 343 246 3 34 375 50 384 8.71e-28 113
MS.gene004581.t1 MTR_8g081000 26.557 305 216 2 72 375 87 384 1.10e-27 113
MS.gene004581.t1 MTR_4g049400 46.923 130 56 4 19 139 25 150 1.63e-27 108
MS.gene004581.t1 MTR_2g436380 70.492 61 18 0 214 274 1 61 2.41e-23 99.0
MS.gene004581.t1 MTR_3g085240 35.583 163 90 6 1 161 21 170 4.41e-15 74.7
MS.gene004581.t1 MTR_3g085240 64.000 50 18 0 273 322 176 225 4.50e-15 74.7
MS.gene004581.t1 MTR_8g012210 22.261 283 198 8 84 355 62 333 7.18e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004581.t1 AT5G07900 32.891 377 239 6 2 367 26 399 3.88e-61 202
MS.gene004581.t1 AT1G21150 30.968 310 207 3 30 335 10 316 3.87e-43 154
MS.gene004581.t1 AT1G21150 32.000 300 197 3 40 335 56 352 6.99e-43 154
MS.gene004581.t1 AT1G21150 32.000 300 197 3 40 335 120 416 3.03e-42 154
MS.gene004581.t1 AT1G61980 29.888 358 200 9 40 355 52 400 1.86e-31 124
MS.gene004581.t1 AT1G61980 29.888 358 200 9 40 355 52 400 1.86e-31 124
MS.gene004581.t1 AT1G61980 29.888 358 200 9 40 355 52 400 1.86e-31 124
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.42e-31 123
MS.gene004581.t1 AT1G61990 28.701 331 195 5 41 338 53 375 1.97e-30 120
MS.gene004581.t1 AT1G61990 28.701 331 195 5 41 338 53 375 1.97e-30 120
MS.gene004581.t1 AT5G64950 27.832 309 205 5 30 326 26 328 8.49e-30 119
MS.gene004581.t1 AT1G62120 29.600 375 204 13 7 331 17 381 1.10e-29 119
MS.gene004581.t1 AT3G46950 31.119 286 180 8 7 286 17 291 7.79e-27 111
MS.gene004581.t1 AT1G62085 26.781 407 204 13 8 330 18 414 2.90e-26 110
MS.gene004581.t1 AT1G62085 26.781 407 204 13 8 330 18 414 2.90e-26 110
MS.gene004581.t1 AT5G23930 26.509 381 202 8 40 350 55 427 1.04e-24 105
MS.gene004581.t1 AT1G62010 27.797 295 169 4 75 331 71 359 2.53e-24 103
MS.gene004581.t1 AT1G62110 28.421 285 191 6 7 284 17 295 3.84e-23 100
MS.gene004581.t1 AT1G61960 29.839 248 164 4 41 284 53 294 1.73e-21 95.9
MS.gene004581.t1 AT1G62150 25.581 258 175 4 34 283 52 300 1.29e-19 90.5
MS.gene004581.t1 AT1G79220 23.975 317 220 10 76 380 82 389 9.31e-12 66.6

Find 85 sgRNAs with CRISPR-Local

Find 104 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTCAGGCTTGCTTTA+AGG 0.167218 2.2:-52182908 None:intergenic
AGGCTTGCTTTAAGGATCTT+AGG 0.180668 2.2:-52182900 None:intergenic
TTAATTTACTCATGAACTTT+TGG 0.214050 2.2:+52183345 MS.gene004581:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2.2:+52182802 MS.gene004581:CDS
GGTGGAGGAGTTAAAGGATT+TGG 0.226563 2.2:+52183148 MS.gene004581:CDS
CTCACCTTCTTTAGAAACTA+TGG 0.240194 2.2:+52182729 MS.gene004581:CDS
GAAATTGGTGGAGGAGTTAA+AGG 0.263607 2.2:+52183142 MS.gene004581:CDS
GTGGAGGAGTTAAAGGATTT+GGG 0.277418 2.2:+52183149 MS.gene004581:CDS
ATTGTAAGTGGAGGAAAGTT+TGG 0.277663 2.2:-52182670 None:intergenic
GTTTCTTGATACATGTATAA+AGG 0.301142 2.2:+52183541 MS.gene004581:CDS
ATTCGCAATCCATCTTTCTT+TGG 0.319814 2.2:+52182999 MS.gene004581:CDS
AAAGAGGGTTAGGGTTTAGA+AGG 0.320241 2.2:-52182533 None:intergenic
AGACCAACCCCACTTCTTAA+AGG 0.324708 2.2:-52183258 None:intergenic
CCATGTTTAAGAATATATTA+TGG 0.338291 2.2:+52182496 None:intergenic
GACACATGACATGCTGAAAT+TGG 0.353711 2.2:+52183127 MS.gene004581:CDS
TACATGTATAAAGGGCATTA+AGG 0.358969 2.2:+52183550 MS.gene004581:CDS
GGCTTGCTTTAAGGATCTTA+GGG 0.360892 2.2:-52182899 None:intergenic
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.2:+52183364 MS.gene004581:CDS
TAATGTTGGAAAAGAGGGTT+AGG 0.372467 2.2:-52182543 None:intergenic
ATGTTCTACAAGCGTTTAGA+AGG 0.384175 2.2:+52183288 MS.gene004581:CDS
TTTCTTGATACATGTATAAA+GGG 0.388445 2.2:+52183542 MS.gene004581:CDS
ACTCCTTCAAAATCAACCTT+TGG 0.392434 2.2:+52183176 MS.gene004581:CDS
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2.2:-52182778 None:intergenic
GTCAGAGTTAGAAGCACCTT+TGG 0.395726 2.2:-52182853 None:intergenic
CTGAAGTGTTGGTGCAGAAA+TGG 0.408928 2.2:-52182584 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2.2:+52183464 MS.gene004581:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2.2:-52182763 None:intergenic
AGCTTCTCAGGCACTACAAA+TGG 0.425476 2.2:-52183518 None:intergenic
GAATCTATAAATCAATTGAT+AGG 0.434987 2.2:-52182943 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2.2:+52183580 MS.gene004581:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2.2:+52183420 MS.gene004581:CDS
AAAAGGGTAATGAGTAATGT+TGG 0.457470 2.2:-52182557 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2.2:-52182764 None:intergenic
AATGCTTTGCGTAAGGAGTC+AGG 0.470114 2.2:+52183091 MS.gene004581:CDS
GTATCAAGAAACAGCTTCTC+AGG 0.470819 2.2:-52183530 None:intergenic
GAAAGTTTGGAAGCAGATTG+TGG 0.472463 2.2:-52182657 None:intergenic
CTTGAAACAAACATTGTAAG+TGG 0.473763 2.2:-52182682 None:intergenic
AAGAAGGTGAGAACAGAGTC+AGG 0.478602 2.2:-52182717 None:intergenic
TCCTACCTCATCAACAACTT+TGG 0.478665 2.2:+52182627 MS.gene004581:CDS
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.2:+52183363 MS.gene004581:CDS
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2.2:+52182803 MS.gene004581:CDS
GTGCAGAAATGGAGAGAAAA+GGG 0.484129 2.2:-52182573 None:intergenic
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2.2:-52183060 None:intergenic
AAAGTTTGGAAGCAGATTGT+GGG 0.488544 2.2:-52182656 None:intergenic
GAAACCATAGTTTCTAAAGA+AGG 0.490559 2.2:-52182733 None:intergenic
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2.2:+52183421 MS.gene004581:CDS
AATGAGTAATGTTGGAAAAG+AGG 0.500309 2.2:-52182549 None:intergenic
GGTGCAGAAATGGAGAGAAA+AGG 0.506359 2.2:-52182574 None:intergenic
CCATAATATATTCTTAAACA+TGG 0.509202 2.2:-52182496 None:intergenic
ATGAGTAATGTTGGAAAAGA+GGG 0.517078 2.2:-52182548 None:intergenic
TTAAAAGAATGCTTTGCGTA+AGG 0.521144 2.2:+52183084 MS.gene004581:CDS
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2.2:+52183432 MS.gene004581:CDS
ATTATATTGCTGATTGCCAA+TGG 0.523498 2.2:+52183044 MS.gene004581:CDS
CGAGCGTCTCCAAAGAAAGA+TGG 0.526080 2.2:-52183008 None:intergenic
AAACTGCTGGTCCTTTGGCA+AGG 0.527369 2.2:-52183397 None:intergenic
AAGAGGGTTAGGGTTTAGAA+GGG 0.536351 2.2:-52182532 None:intergenic
ACTACGTCTTACCATTTCAA+AGG 0.541064 2.2:+52182767 MS.gene004581:CDS
GAGGTAGGAGACTGCAAAAG+AGG 0.555968 2.2:-52182613 None:intergenic
AGTCGATGCCTTTAAGAAGT+GGG 0.559365 2.2:+52183250 MS.gene004581:CDS
AACTGCTGGTCCTTTGGCAA+GGG 0.562420 2.2:-52183396 None:intergenic
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2.2:+52183386 MS.gene004581:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2.2:-52183449 None:intergenic
AATGTTGGAAAAGAGGGTTA+GGG 0.572620 2.2:-52182542 None:intergenic
GGCTAGCAATGCTACACCAA+AGG 0.575700 2.2:-52183192 None:intergenic
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2.2:+52183433 MS.gene004581:CDS
AAGTCGATGCCTTTAAGAAG+TGG 0.577016 2.2:+52183249 MS.gene004581:CDS
TAGATTCTTGCAATCAGACA+AGG 0.583953 2.2:+52182959 MS.gene004581:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2.2:-52183445 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.598873 2.2:+52183255 MS.gene004581:CDS
GCCAAAGTTGTTGATGAGGT+AGG 0.602091 2.2:-52182628 None:intergenic
GTCGATGCCTTTAAGAAGTG+GGG 0.602513 2.2:+52183251 MS.gene004581:CDS
ACATGACATGCTGAAATTGG+TGG 0.610610 2.2:+52183130 MS.gene004581:CDS
AAGTTTGGAAGCAGATTGTG+GGG 0.614108 2.2:-52182655 None:intergenic
AGAGGTTGAATCTGAAGTGT+TGG 0.619487 2.2:-52182595 None:intergenic
AAAAGCCAAAGTTGTTGATG+AGG 0.632517 2.2:-52182632 None:intergenic
TTTCTTTGGAGACGCTCGTG+TGG 0.632941 2.2:+52183013 MS.gene004581:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2.2:+52183607 MS.gene004581:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2.2:-52183125 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2.2:-52182792 None:intergenic
TGACATGCTGAAATTGGTGG+AGG 0.646237 2.2:+52183133 MS.gene004581:CDS
GAAACAAACATTGTAAGTGG+AGG 0.650981 2.2:-52182679 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2.2:+52182775 MS.gene004581:CDS
ATGGATGTTAACATACAATG+AGG 0.655070 2.2:-52183314 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.669284 2.2:+52183049 MS.gene004581:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2.2:-52183456 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGATAATTTTATTATTC+AGG - chr2.2:52182923-52182942 None:intergenic 15.0%
!!! GAGTAAATTAATTTTATCAA+TGG - chr2.2:52183336-52183355 None:intergenic 15.0%
!! GAATCTATAAATCAATTGAT+AGG - chr2.2:52182946-52182965 None:intergenic 20.0%
!! TTATCTATTAAAGTTGTATG+AGG + chr2.2:52183580-52183599 MS.gene004581:CDS 20.0%
!! TTTCTTGATACATGTATAAA+GGG + chr2.2:52183542-52183561 MS.gene004581:CDS 20.0%
!!! TAATTTACTCATGAACTTTT+GGG + chr2.2:52183346-52183365 MS.gene004581:CDS 20.0%
!!! TTAATTTACTCATGAACTTT+TGG + chr2.2:52183345-52183364 MS.gene004581:CDS 20.0%
! AAACTTAAAGAAAAAACTGC+TGG - chr2.2:52183413-52183432 None:intergenic 25.0%
! ATCTGTAAGAAAAACATTGT+GGG + chr2.2:52183220-52183239 MS.gene004581:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.2:52182834-52182853 None:intergenic 25.0%
! GTTTCTTGATACATGTATAA+AGG + chr2.2:52183541-52183560 MS.gene004581:CDS 25.0%
! TATCTGTAAGAAAAACATTG+TGG + chr2.2:52183219-52183238 MS.gene004581:CDS 25.0%
!! CATTCTTTTAATGTTTGAGT+CGG - chr2.2:52183075-52183094 None:intergenic 25.0%
!! TGTTTTTCTTACAGATAGTT+TGG - chr2.2:52183216-52183235 None:intergenic 25.0%
!! TTCTTTAAGTTTCGAAAAGA+GGG + chr2.2:52183421-52183440 MS.gene004581:CDS 25.0%
!! TTTCTTTAAGTTTCGAAAAG+AGG + chr2.2:52183420-52183439 MS.gene004581:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.2:52182837-52182856 MS.gene004581:CDS 25.0%
AAAAGGGTAATGAGTAATGT+TGG - chr2.2:52182560-52182579 None:intergenic 30.0%
AATGAGTAATGTTGGAAAAG+AGG - chr2.2:52182552-52182571 None:intergenic 30.0%
ATGAGTAATGTTGGAAAAGA+GGG - chr2.2:52182551-52182570 None:intergenic 30.0%
ATGGATGTTAACATACAATG+AGG - chr2.2:52183317-52183336 None:intergenic 30.0%
CTTGAAACAAACATTGTAAG+TGG - chr2.2:52182685-52182704 None:intergenic 30.0%
TCTGTAAGAAAAACATTGTG+GGG + chr2.2:52183221-52183240 MS.gene004581:CDS 30.0%
TTAAAAGAATGCTTTGCGTA+AGG + chr2.2:52183084-52183103 MS.gene004581:CDS 30.0%
! ATTATATTGCTGATTGCCAA+TGG + chr2.2:52183044-52183063 MS.gene004581:CDS 30.0%
! GAAACCATAGTTTCTAAAGA+AGG - chr2.2:52182736-52182755 None:intergenic 30.0%
! TATTATTCAGGCTTGCTTTA+AGG - chr2.2:52182911-52182930 None:intergenic 30.0%
! TTGCAATATCTTCTGAAGAA+AGG + chr2.2:52183464-52183483 MS.gene004581:CDS 30.0%
!! TACATGTATAAAGGGCATTA+AGG + chr2.2:52183550-52183569 MS.gene004581:CDS 30.0%
AAAAGCCAAAGTTGTTGATG+AGG - chr2.2:52182635-52182654 None:intergenic 35.0%
AAAGTTTGGAAGCAGATTGT+GGG - chr2.2:52182659-52182678 None:intergenic 35.0%
AATGTTGGAAAAGAGGGTTA+GGG - chr2.2:52182545-52182564 None:intergenic 35.0%
AATTTCAGCATGTCATGTGT+CGG - chr2.2:52183128-52183147 None:intergenic 35.0%
ACTACGTCTTACCATTTCAA+AGG + chr2.2:52182767-52182786 MS.gene004581:CDS 35.0%
ACTCCTTCAAAATCAACCTT+TGG + chr2.2:52183176-52183195 MS.gene004581:CDS 35.0%
ATGTTCTACAAGCGTTTAGA+AGG + chr2.2:52183288-52183307 MS.gene004581:CDS 35.0%
ATTCGCAATCCATCTTTCTT+TGG + chr2.2:52182999-52183018 MS.gene004581:CDS 35.0%
ATTGTAAGTGGAGGAAAGTT+TGG - chr2.2:52182673-52182692 None:intergenic 35.0%
CAGAAGATATTGCAATACAG+AGG - chr2.2:52183459-52183478 None:intergenic 35.0%
GAAACAAACATTGTAAGTGG+AGG - chr2.2:52182682-52182701 None:intergenic 35.0%
TAATGTTGGAAAAGAGGGTT+AGG - chr2.2:52182546-52182565 None:intergenic 35.0%
TAGATTCTTGCAATCAGACA+AGG + chr2.2:52182959-52182978 MS.gene004581:CDS 35.0%
TGAAATGGTAAGACGTAGTT+GGG - chr2.2:52182766-52182785 None:intergenic 35.0%
TTACCATTTCAAAGGAACCA+TGG + chr2.2:52182775-52182794 MS.gene004581:CDS 35.0%
TTGAAATGGTAAGACGTAGT+TGG - chr2.2:52182767-52182786 None:intergenic 35.0%
! CTCACCTTCTTTAGAAACTA+TGG + chr2.2:52182729-52182748 MS.gene004581:CDS 35.0%
! TTTGGCAAAACCCTTTTAGA+AGG - chr2.2:52182816-52182835 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG + chr2.2:52183359-52183378 MS.gene004581:CDS 35.0%
!! ACACCAAAGGTTGATTTTGA+AGG - chr2.2:52183182-52183201 None:intergenic 35.0%
!! TTGGCAAAACCCTTTTAGAA+GGG - chr2.2:52182815-52182834 None:intergenic 35.0%
AAAGAGGGTTAGGGTTTAGA+AGG - chr2.2:52182536-52182555 None:intergenic 40.0%
AAGAGGGTTAGGGTTTAGAA+GGG - chr2.2:52182535-52182554 None:intergenic 40.0%
AAGTTTGGAAGCAGATTGTG+GGG - chr2.2:52182658-52182677 None:intergenic 40.0%
ACATGACATGCTGAAATTGG+TGG + chr2.2:52183130-52183149 MS.gene004581:CDS 40.0%
ACTCAATCTTGCTTACACCA+TGG + chr2.2:52183607-52183626 MS.gene004581:CDS 40.0%
AGAAAAAACTGCTGGTCCTT+TGG - chr2.2:52183405-52183424 None:intergenic 40.0%
GAAAGTTTGGAAGCAGATTG+TGG - chr2.2:52182660-52182679 None:intergenic 40.0%
GAAATTGGTGGAGGAGTTAA+AGG + chr2.2:52183142-52183161 MS.gene004581:CDS 40.0%
GACACATGACATGCTGAAAT+TGG + chr2.2:52183127-52183146 MS.gene004581:CDS 40.0%
GTATCAAGAAACAGCTTCTC+AGG - chr2.2:52183533-52183552 None:intergenic 40.0%
GTGCAGAAATGGAGAGAAAA+GGG - chr2.2:52182576-52182595 None:intergenic 40.0%
GTGGAGGAGTTAAAGGATTT+GGG + chr2.2:52183149-52183168 MS.gene004581:CDS 40.0%
TCCTACCTCATCAACAACTT+TGG + chr2.2:52182627-52182646 MS.gene004581:CDS 40.0%
TTTCCTGCAACCCTTCTAAA+AGG + chr2.2:52182802-52182821 MS.gene004581:CDS 40.0%
! AAGTCGATGCCTTTAAGAAG+TGG + chr2.2:52183249-52183268 MS.gene004581:CDS 40.0%
! AGAGGTTGAATCTGAAGTGT+TGG - chr2.2:52182598-52182617 None:intergenic 40.0%
! AGTCGATGCCTTTAAGAAGT+GGG + chr2.2:52183250-52183269 MS.gene004581:CDS 40.0%
! GAGAACAGAGTCAGGTTTTT+GGG - chr2.2:52182712-52182731 None:intergenic 40.0%
! TAGCCATGGTTCCTTTGAAA+TGG - chr2.2:52182781-52182800 None:intergenic 40.0%
! TGAGAACAGAGTCAGGTTTT+TGG - chr2.2:52182713-52182732 None:intergenic 40.0%
! TGATGTGCATGTTTTGTCCA+TGG - chr2.2:52183627-52183646 None:intergenic 40.0%
! TTCCTGCAACCCTTCTAAAA+GGG + chr2.2:52182803-52182822 MS.gene004581:CDS 40.0%
!! AGGCTTGCTTTAAGGATCTT+AGG - chr2.2:52182903-52182922 None:intergenic 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG + chr2.2:52183358-52183377 MS.gene004581:CDS 40.0%
!! GGCTTGCTTTAAGGATCTTA+GGG - chr2.2:52182902-52182921 None:intergenic 40.0%
AAGAAGGTGAGAACAGAGTC+AGG - chr2.2:52182720-52182739 None:intergenic 45.0%
AATGCTTTGCGTAAGGAGTC+AGG + chr2.2:52183091-52183110 MS.gene004581:CDS 45.0%
AGACCAACCCCACTTCTTAA+AGG - chr2.2:52183261-52183280 None:intergenic 45.0%
AGCTTCTCAGGCACTACAAA+TGG - chr2.2:52183521-52183540 None:intergenic 45.0%
GGTGCAGAAATGGAGAGAAA+AGG - chr2.2:52182577-52182596 None:intergenic 45.0%
GGTGGAGGAGTTAAAGGATT+TGG + chr2.2:52183148-52183167 MS.gene004581:CDS 45.0%
TATTGCAATACAGAGGCCCT+TGG - chr2.2:52183452-52183471 None:intergenic 45.0%
TGACATGCTGAAATTGGTGG+AGG + chr2.2:52183133-52183152 MS.gene004581:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG + chr2.2:52183364-52183383 MS.gene004581:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG + chr2.2:52183363-52183382 MS.gene004581:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG + chr2.2:52183255-52183274 MS.gene004581:CDS 45.0%
! ATTGCTGATTGCCAATGGAG+TGG + chr2.2:52183049-52183068 MS.gene004581:CDS 45.0%
! GCCAAAGTTGTTGATGAGGT+AGG - chr2.2:52182631-52182650 None:intergenic 45.0%
! GTCGATGCCTTTAAGAAGTG+GGG + chr2.2:52183251-52183270 MS.gene004581:CDS 45.0%
!! AACCCTTTTAGAAGGGTTGC+AGG - chr2.2:52182808-52182827 None:intergenic 45.0%
!! CTGAAGTGTTGGTGCAGAAA+TGG - chr2.2:52182587-52182606 None:intergenic 45.0%
!! GTCAGAGTTAGAAGCACCTT+TGG - chr2.2:52182856-52182875 None:intergenic 45.0%
AAACTGCTGGTCCTTTGGCA+AGG - chr2.2:52183400-52183419 None:intergenic 50.0%
AACTGCTGGTCCTTTGGCAA+GGG - chr2.2:52183399-52183418 None:intergenic 50.0%
CGAAAAGAGGGTCCTTCCAA+GGG + chr2.2:52183433-52183452 MS.gene004581:CDS 50.0%
CGAGCGTCTCCAAAGAAAGA+TGG - chr2.2:52183011-52183030 None:intergenic 50.0%
GAGGTAGGAGACTGCAAAAG+AGG - chr2.2:52182616-52182635 None:intergenic 50.0%
GGCTAGCAATGCTACACCAA+AGG - chr2.2:52183195-52183214 None:intergenic 50.0%
TCGAAAAGAGGGTCCTTCCA+AGG + chr2.2:52183432-52183451 MS.gene004581:CDS 50.0%
! TTTCTTTGGAGACGCTCGTG+TGG + chr2.2:52183013-52183032 MS.gene004581:CDS 50.0%
GATGCACTTGCCCTTGCCAA+AGG + chr2.2:52183386-52183405 MS.gene004581:CDS 55.0%
GCAATACAGAGGCCCTTGGA+AGG - chr2.2:52183448-52183467 None:intergenic 55.0%
GGGTTGCAGGAAAGTAGCCA+TGG - chr2.2:52182795-52182814 None:intergenic 55.0%
GTTTGAGTCGGCCACTCCAT+TGG - chr2.2:52183063-52183082 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52182498 52183649 52182498 ID=MS.gene004581
chr2.2 mRNA 52182498 52183649 52182498 ID=MS.gene004581.t1;Parent=MS.gene004581
chr2.2 exon 52182498 52183649 52182498 ID=MS.gene004581.t1.exon1;Parent=MS.gene004581.t1
chr2.2 CDS 52182498 52183649 52182498 ID=cds.MS.gene004581.t1;Parent=MS.gene004581.t1
Gene Sequence

>MS.gene004581

ATGTTTAAGAATATATTATGGCGACACAGAACAACCCTTCTAAACCCTAACCCTCTTTTCCAACATTACTCATTACCCTTTTCTCTCCATTTCTGCACCAACACTTCAGATTCAACCTCTTTTGCAGTCTCCTACCTCATCAACAACTTTGGCTTTTCCCCACAATCTGCTTCCAAACTTTCCTCCACTTACAATGTTTGTTTCAAGAATGCCCAAAAACCTGACTCTGTTCTCACCTTCTTTAGAAACTATGGTTTCTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTAACTCTGACATTGTTAACATTGTCACTAAGAACCCTAAGATCCTTAAAGCAAGCCTGAATAATAAAATTATCAAAGCCTATCAATTGATTTATAGATTCTTGCAATCAGACAAGGTCATTATTGATTGTGTTATTCGCAATCCATCTTTCTTTGGAGACGCTCGTGTGGTATCTAACATTATATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGCGTAAGGAGTCAGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAGTTAAAGGATTTGGGTTTTACTCCTTCAAAATCAACCTTTGGTGTAGCATTGCTAGCCAAACTATCTGTAAGAAAAACATTGTGGGGAGAGAAAGTCGATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCGTTTAGAAGGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTACTCATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCACTTGCCCTTGCCAAAGGACCAGCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAACAAGAATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAGAAGCTGTTTCTTGATACATGTATAAAGGGCATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCATGA
Protein sequence

>MS.gene004581.t1

MFKNILWRHRTTLLNPNPLFQHYSLPFSLHFCTNTSDSTSFAVSYLINNFGFSPQSASKLSSTYNVCFKNAQKPDSVLTFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSKGASNSDIVNIVTKNPKILKASLNNKIIKAYQLIYRFLQSDKVIIDCVIRNPSFFGDARVVSNIILLIANGVADSNIKRMLCVRSQAFQTPTHDMLKLVEELKDLGFTPSKSTFGVALLAKLSVRKTLWGEKVDAFKKWGWSDEDVLQAFRRQPHCMLTSIDKINLLMNFWVNQLGWDALALAKGPAVFSLSFEKRVLPRASVLQYLLKKGLRNKNASLTCPFVVPEKLFLDTCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS