AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00327

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00327.t1 MTR_2g437130 95.472 265 12 0 46 310 123 387 0.0 521
MS.gene00327.t1 MTR_2g437200 69.721 251 73 1 46 293 147 397 2.07e-124 362
MS.gene00327.t1 MTR_2g437170 65.428 269 88 2 44 307 135 403 2.39e-121 354
MS.gene00327.t1 MTR_2g437150 63.469 271 94 2 44 309 135 405 3.22e-119 348
MS.gene00327.t1 MTR_2g076320 63.704 270 95 1 44 310 130 399 8.89e-119 347
MS.gene00327.t1 MTR_2g437990 63.433 268 96 1 45 310 131 398 2.42e-117 345
MS.gene00327.t1 MTR_2g437960 61.567 268 100 1 46 310 135 402 1.02e-116 342
MS.gene00327.t1 MTR_2g437960 61.567 268 100 1 46 310 135 402 1.87e-116 342
MS.gene00327.t1 MTR_2g437160 60.902 266 102 1 45 308 144 409 3.77e-116 341
MS.gene00327.t1 MTR_2g438020 60.211 284 99 3 40 310 170 452 1.32e-115 342
MS.gene00327.t1 MTR_2g437120 63.704 270 94 2 42 308 129 397 2.69e-115 339
MS.gene00327.t1 MTR_2g438010 60.821 268 102 1 46 310 152 419 1.34e-114 337
MS.gene00327.t1 MTR_2g437240 63.433 268 95 1 46 310 147 414 2.09e-114 337
MS.gene00327.t1 MTR_2g437180 59.551 267 103 2 46 309 131 395 5.88e-110 325
MS.gene00327.t1 MTR_2g437940 57.194 278 108 2 44 310 93 370 1.59e-106 315
MS.gene00327.t1 MTR_2g437100 60.311 257 97 2 42 293 138 394 7.30e-103 307
MS.gene00327.t1 MTR_2g437080 52.529 257 122 0 46 302 122 378 8.12e-93 280
MS.gene00327.t1 MTR_2g437160 63.415 205 72 1 107 308 114 318 2.42e-91 275
MS.gene00327.t1 MTR_2g436440 51.724 261 126 0 46 306 128 388 4.65e-90 274
MS.gene00327.t1 MTR_2g437060 51.751 257 124 0 46 302 137 393 1.09e-87 268
MS.gene00327.t1 MTR_2g437020 54.054 259 116 2 46 302 121 378 6.16e-87 265
MS.gene00327.t1 MTR_4g119550 51.724 261 126 0 42 302 128 388 3.33e-86 264
MS.gene00327.t1 MTR_2g437030 49.425 261 132 0 46 306 208 468 7.02e-86 266
MS.gene00327.t1 MTR_2g437040 47.893 261 136 0 46 306 122 382 1.85e-81 251
MS.gene00327.t1 MTR_2g436400 47.727 264 116 3 46 298 129 381 8.83e-80 247
MS.gene00327.t1 MTR_2g437260 42.806 278 148 4 42 310 112 387 5.56e-77 240
MS.gene00327.t1 MTR_2g436460 50.195 257 127 1 42 297 123 379 6.46e-76 237
MS.gene00327.t1 MTR_4g119580 44.223 251 140 0 42 292 120 370 1.76e-65 210
MS.gene00327.t1 MTR_4g120380 42.629 251 144 0 42 292 120 370 8.81e-63 203
MS.gene00327.t1 MTR_2g437090 52.353 170 81 0 138 307 2 171 5.90e-61 192
MS.gene00327.t1 MTR_4g120380 43.290 231 131 0 42 272 120 350 1.98e-57 191
MS.gene00327.t1 MTR_2g019810 41.603 262 149 3 42 301 107 366 2.06e-57 189
MS.gene00327.t1 MTR_2g019840 38.846 260 155 3 42 299 119 376 9.28e-53 177
MS.gene00327.t1 MTR_4g119570 37.097 248 123 1 47 294 17 231 3.48e-48 161
MS.gene00327.t1 MTR_3g450500 28.458 253 175 2 46 292 118 370 5.14e-28 112
MS.gene00327.t1 MTR_3g085240 58.889 90 20 1 196 285 176 248 1.75e-26 105
MS.gene00327.t1 MTR_4g007490 31.102 254 166 4 46 292 117 368 2.21e-26 107
MS.gene00327.t1 MTR_8g081000 26.000 250 182 2 46 292 136 385 2.32e-21 93.6
MS.gene00327.t1 MTR_8g081000 26.000 250 182 2 46 292 136 385 2.38e-21 93.6
MS.gene00327.t1 MTR_8g081000 26.000 250 182 2 46 292 136 385 3.51e-21 93.2
MS.gene00327.t1 MTR_2g436380 61.017 59 23 0 138 196 2 60 3.94e-19 85.9
MS.gene00327.t1 MTR_8g080990 26.423 246 178 2 46 288 123 368 5.11e-18 84.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00327.t1 AT5G07900 32.016 253 164 4 46 291 148 399 8.65e-37 136
MS.gene00327.t1 AT1G21150 28.235 255 177 3 46 294 99 353 2.00e-29 115
MS.gene00327.t1 AT1G21150 28.235 255 177 3 46 294 135 389 3.18e-29 115
MS.gene00327.t1 AT1G21150 28.235 255 177 3 46 294 199 453 6.98e-29 115
MS.gene00327.t1 AT5G64950 26.623 308 202 7 4 292 74 376 9.86e-20 89.0
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G61970 29.137 278 150 8 42 277 127 399 1.00e-17 83.6
MS.gene00327.t1 AT1G62010 27.106 273 160 5 42 277 113 383 2.17e-17 82.4
MS.gene00327.t1 AT1G61980 27.636 275 158 6 42 277 127 399 3.05e-16 79.0
MS.gene00327.t1 AT1G61980 27.636 275 158 6 42 277 127 399 3.05e-16 79.0
MS.gene00327.t1 AT1G61980 27.636 275 158 6 42 277 127 399 3.05e-16 79.0
MS.gene00327.t1 AT1G62110 29.121 182 122 3 31 207 116 295 1.22e-11 65.5
MS.gene00327.t1 AT3G46950 28.804 184 120 4 31 210 116 292 5.92e-11 63.5

Find 56 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTATTCTGTAAAATTCT+AGG 0.190911 2.1:+56223479 None:intergenic
GTTAAGTTATTGATTGATTA+TGG 0.197960 2.1:-56223335 MS.gene00327:CDS
TTCAATTGATAAGATTAATT+TGG 0.211307 2.1:-56223054 MS.gene00327:CDS
TTGGAGGAAGTTAAGGGTTT+AGG 0.213631 2.1:-56223236 MS.gene00327:CDS
GACCCTTCAACTTCAACTTT+TGG 0.225478 2.1:-56223209 MS.gene00327:CDS
TGGAGGAAGTTAAGGGTTTA+GGG 0.238470 2.1:-56223235 MS.gene00327:CDS
ATTCTTTGGCACTTTCTAAA+AGG 0.258538 2.1:-56222998 MS.gene00327:CDS
AGATTATGAAGTCACCTATT+TGG 0.271993 2.1:+56222731 None:intergenic
TCAATTGGGTTGGAATTCTT+TGG 0.276844 2.1:-56223012 MS.gene00327:CDS
TAGTACTTTGGAGGAAGTTA+AGG 0.340131 2.1:-56223243 MS.gene00327:CDS
TGACATGCCATTCACCAAAT+AGG 0.363432 2.1:-56222745 None:intergenic
ATTGAAGTCAACAACAATTC+AGG 0.371659 2.1:+56223071 None:intergenic
CTTCGCTTCACAGTTCTTTG+AGG 0.374046 2.1:-56224143 MS.gene00327:CDS
TTAGTTACAGTTTGCAGAAA+AGG 0.377592 2.1:-56222965 MS.gene00327:CDS
TGAACCGTTTGCTCCGTCTA+AGG 0.377750 2.1:-56222871 MS.gene00327:CDS
TTGACAAGACCAGCTATACT+TGG 0.394866 2.1:-56223281 MS.gene00327:CDS
AATTGATTTCCTTAGTACTT+TGG 0.395237 2.1:-56223255 MS.gene00327:CDS
GATGTTATTCGAGCATTTAG+AGG 0.411519 2.1:-56223098 MS.gene00327:CDS
ATGTATGACTAAAACACTTT+GGG 0.428463 2.1:-56223165 MS.gene00327:CDS
AGTACTTTGGAGGAAGTTAA+GGG 0.430815 2.1:-56223242 MS.gene00327:CDS
GAAGTCACCTATTTGGTGAA+TGG 0.443693 2.1:+56222738 None:intergenic
AATGGATGTGGTTGTTGAAA+TGG 0.453420 2.1:-56225354 MS.gene00327:CDS
CTGCAATTTCTTGTGATGAA+AGG 0.454596 2.1:-56222921 MS.gene00327:CDS
AATGTATGACTAAAACACTT+TGG 0.458815 2.1:-56223166 MS.gene00327:CDS
AACTGAGAACATATTTGCTT+AGG 0.466357 2.1:+56222841 None:intergenic
TTATTTATTAAAGCTTTATG+AGG 0.468070 2.1:-56222802 MS.gene00327:CDS
TTAACAGTAGTAAGATTGTA+AGG 0.474106 2.1:+56223359 None:intergenic
CATGTCAATGTTCTCCTTGG+TGG 0.478386 2.1:+56222761 None:intergenic
TTCTTGTGATGAAAGGTTTG+AGG 0.479601 2.1:-56222914 MS.gene00327:CDS
AAGGATTGTTCCAAGGGCAT+TGG 0.488885 2.1:-56222946 MS.gene00327:CDS
TCAATTGATCCAAGTATAGC+TGG 0.498779 2.1:+56223272 None:intergenic
TGATTTCCTTAGTACTTTGG+AGG 0.499163 2.1:-56223252 MS.gene00327:CDS
TTGGACAGGGGACCTTCCGC+CGG 0.499873 2.1:-56224184 MS.gene00327:intron
TCTGATACCATGATTGCATA+AGG 0.500320 2.1:+56224108 None:intergenic
CTTAGGAACATTTCCTTAGA+CGG 0.503257 2.1:+56222858 None:intergenic
TAACTTCCTCCAAAGTACTA+AGG 0.517307 2.1:+56223246 None:intergenic
CAACATAATTACACAGCTGA+GGG 0.525854 2.1:+56225311 None:intergenic
ATTTCCTTAGACGGAGCAAA+CGG 0.526804 2.1:+56222867 None:intergenic
TTTGGGTTAATCAATTGGGT+TGG 0.527975 2.1:-56223022 MS.gene00327:CDS
AGCAAACGGTTCAACTAAGC+TGG 0.533205 2.1:+56222881 None:intergenic
GACCCAAAAGTTGAAGTTGA+AGG 0.537017 2.1:+56223206 None:intergenic
AATTGCAGAACCAATGCCCT+TGG 0.540852 2.1:+56222936 None:intergenic
TGCAGAAAAGGATTGTTCCA+AGG 0.541911 2.1:-56222953 MS.gene00327:CDS
GCAGAAAAGGATTGTTCCAA+GGG 0.547363 2.1:-56222952 MS.gene00327:CDS
TGGCATGTCAATGTTCTCCT+TGG 0.547892 2.1:+56222758 None:intergenic
TCCGCCGGTGTCATCTCTGA+AGG 0.562552 2.1:-56224169 MS.gene00327:CDS
ACCCAAAAGTTGAAGTTGAA+GGG 0.582729 2.1:+56223207 None:intergenic
CAAAAGCAATCTAGCAATAG+CGG 0.586332 2.1:+56223301 None:intergenic
GGATGTGGTTGTTGAAATGG+TGG 0.589673 2.1:-56225351 MS.gene00327:CDS
TCAACATAATTACACAGCTG+AGG 0.591713 2.1:+56225310 None:intergenic
TCAGAGATGACACCGGCGGA+AGG 0.594445 2.1:+56224172 None:intergenic
GGTTGTTGAAATGGTGGACG+AGG 0.634442 2.1:-56225345 MS.gene00327:CDS
TCTGAATTGAGAAATGGATG+TGG 0.659510 2.1:-56225366 None:intergenic
CGTAAATCTTGCATCCACCA+AGG 0.669439 2.1:-56222775 MS.gene00327:CDS
TCCTTCAGAGATGACACCGG+CGG 0.706068 2.1:+56224168 None:intergenic
GAGTCCTTCAGAGATGACAC+CGG 0.731986 2.1:+56224165 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAAAATTTCGATATAACAA+AGG - chr2.1:56224127-56224146 MS.gene00327:CDS 15.0%
!! TTCAATTGATAAGATTAATT+TGG - chr2.1:56225045-56225064 MS.gene00327:intron 15.0%
!!! TACATATTTTCAGAAAATTA+TGG - chr2.1:56223449-56223468 MS.gene00327:CDS 15.0%
!!! TTATTTATTAAAGCTTTATG+AGG - chr2.1:56225297-56225316 MS.gene00327:intron 15.0%
!!! TTTCAATTTTTTCTATCTAA+AGG - chr2.1:56224557-56224576 MS.gene00327:intron 15.0%
!! AAACTATTCTGTAAAATTCT+AGG + chr2.1:56224623-56224642 None:intergenic 20.0%
!! AAGAAAGTGAAAAATGAATT+GGG + chr2.1:56224440-56224459 None:intergenic 20.0%
!! CATATTTATATATCTGAATG+AGG - chr2.1:56223814-56223833 MS.gene00327:intron 20.0%
!! CTCTTATAAAATTCAAAAAG+AGG + chr2.1:56224662-56224681 None:intergenic 20.0%
!! GAATTGTAATTGTATAATGT+TGG - chr2.1:56223749-56223768 MS.gene00327:intron 20.0%
!! GATAGAAAAAATTGAAACTT+TGG + chr2.1:56224554-56224573 None:intergenic 20.0%
!! TAAACAAATTGTGAATAACT+AGG + chr2.1:56223605-56223624 None:intergenic 20.0%
!! TCAACTAATAAGTTCAAAAT+GGG + chr2.1:56223694-56223713 None:intergenic 20.0%
!! TGGTATTAAAAAGAAAGAAA+AGG + chr2.1:56223350-56223369 None:intergenic 20.0%
!!! AAGTTGATGTTTTTAAGAAA+TGG - chr2.1:56224963-56224982 MS.gene00327:intron 20.0%
!!! AGTTGATGTTTTTAAGAAAT+GGG - chr2.1:56224964-56224983 MS.gene00327:intron 20.0%
!!! CATTTTTAGCAATAAAACAA+TGG + chr2.1:56223557-56223576 None:intergenic 20.0%
!!! CTCTTTTTGAATTTTATAAG+AGG - chr2.1:56224660-56224679 MS.gene00327:intron 20.0%
!!! GTTAAGTTATTGATTGATTA+TGG - chr2.1:56224764-56224783 MS.gene00327:intron 20.0%
!!! TAGAATTTTACAGAATAGTT+TGG - chr2.1:56224622-56224641 MS.gene00327:intron 20.0%
!!! TTATTGGTTAAAATAGTCTT+GGG + chr2.1:56224086-56224105 None:intergenic 20.0%
!!! TTTATTGGTTAAAATAGTCT+TGG + chr2.1:56224087-56224106 None:intergenic 20.0%
! AAAATTGGAATGGGAAAATA+GGG + chr2.1:56224292-56224311 None:intergenic 25.0%
! AAACTGTAACTAAAAATACG+TGG + chr2.1:56225127-56225146 None:intergenic 25.0%
! AATGTATGACTAAAACACTT+TGG - chr2.1:56224933-56224952 MS.gene00327:intron 25.0%
! AGACTAATTTATGACACTAA+AGG + chr2.1:56223125-56223144 None:intergenic 25.0%
! ATGTATGACTAAAACACTTT+GGG - chr2.1:56224934-56224953 MS.gene00327:intron 25.0%
! GAAGAAAGTGAAAAATGAAT+TGG + chr2.1:56224441-56224460 None:intergenic 25.0%
! GTCAACTAATAAGTTCAAAA+TGG + chr2.1:56223695-56223714 None:intergenic 25.0%
! TAGCAATAAAACAATGGAAA+AGG + chr2.1:56223551-56223570 None:intergenic 25.0%
! TATAATGTCTGAAAAATCAG+AGG - chr2.1:56223272-56223291 MS.gene00327:CDS 25.0%
! TCTATTCATGAATCTTGAAT+GGG - chr2.1:56223658-56223677 MS.gene00327:intron 25.0%
! TTAACAGTAGTAAGATTGTA+AGG + chr2.1:56224743-56224762 None:intergenic 25.0%
!! AATTGATTTCCTTAGTACTT+TGG - chr2.1:56224844-56224863 MS.gene00327:intron 25.0%
!! AGTGTCATAAATTAGTCTAT+AGG - chr2.1:56223127-56223146 MS.gene00327:CDS 25.0%
!! CAAATATGTTCTCAGTTTTA+AGG - chr2.1:56225264-56225283 MS.gene00327:intron 25.0%
!! TTCTTGATTTTGCACTTTAT+TGG + chr2.1:56224102-56224121 None:intergenic 25.0%
!!! AGTTTTTGGGTTAATCAATT+GGG - chr2.1:56225073-56225092 MS.gene00327:intron 25.0%
!!! GTTGATGTTTTTAAGAAATG+GGG - chr2.1:56224965-56224984 MS.gene00327:intron 25.0%
!!! TAATTTGGTGTTGAGTTTTT+GGG - chr2.1:56225060-56225079 MS.gene00327:intron 25.0%
!!! TGGAAGTTTTTTTGAACTTT+GGG - chr2.1:56223220-56223239 MS.gene00327:CDS 25.0%
!!! TTAATTTGGTGTTGAGTTTT+TGG - chr2.1:56225059-56225078 MS.gene00327:intron 25.0%
AAATTGGAATGGGAAAATAG+GGG + chr2.1:56224291-56224310 None:intergenic 30.0%
AACTGAGAACATATTTGCTT+AGG + chr2.1:56225261-56225280 None:intergenic 30.0%
AATTTGTGGGAAAATTGGAA+TGG + chr2.1:56224302-56224321 None:intergenic 30.0%
AGAACCCAAATTCTTAAATG+TGG - chr2.1:56222948-56222967 MS.gene00327:CDS 30.0%
ATAGGTGTTTGATGAAATTC+TGG - chr2.1:56222836-56222855 MS.gene00327:CDS 30.0%
ATGAATAGAGCAAGAAACTT+TGG + chr2.1:56223647-56223666 None:intergenic 30.0%
ATTCTTTGGCACTTTCTAAA+AGG - chr2.1:56225101-56225120 MS.gene00327:intron 30.0%
ATTGAAGTCAACAACAATTC+AGG + chr2.1:56225031-56225050 None:intergenic 30.0%
ATTTGTGGGAAAATTGGAAT+GGG + chr2.1:56224301-56224320 None:intergenic 30.0%
CATTGTTTACTGTGACAAAT+AGG - chr2.1:56222818-56222837 MS.gene00327:CDS 30.0%
CTAAAATACACTTGTAGAGT+TGG + chr2.1:56223313-56223332 None:intergenic 30.0%
CTCTATTCATGAATCTTGAA+TGG - chr2.1:56223657-56223676 MS.gene00327:intron 30.0%
GAAAATTGGAATGGGAAAAT+AGG + chr2.1:56224293-56224312 None:intergenic 30.0%
GAACCCAAATTCTTAAATGT+GGG - chr2.1:56222949-56222968 MS.gene00327:CDS 30.0%
TAATTGCTCTGTAATGATGA+TGG + chr2.1:56224244-56224263 None:intergenic 30.0%
TAGGAACTAAGTATACATTG+CGG + chr2.1:56223586-56223605 None:intergenic 30.0%
TAGTTGCAAATTGTCTTGAT+TGG - chr2.1:56223099-56223118 MS.gene00327:CDS 30.0%
TATTGGAATCTGAAAAACCA+AGG + chr2.1:56224469-56224488 None:intergenic 30.0%
TTAGTTACAGTTTGCAGAAA+AGG - chr2.1:56225134-56225153 MS.gene00327:intron 30.0%
TTGTGAATTTGTGGGAAAAT+TGG + chr2.1:56224307-56224326 None:intergenic 30.0%
! AAAAATGAATTGGGCTTTTG+TGG + chr2.1:56224431-56224450 None:intergenic 30.0%
! ACTTTGGAAGAATTGAATTG+TGG + chr2.1:56224538-56224557 None:intergenic 30.0%
! CGTAGATTTTCATTAACGTA+AGG - chr2.1:56223481-56223500 MS.gene00327:CDS 30.0%
! CTTTGGAAGAATTGAATTGT+GGG + chr2.1:56224537-56224556 None:intergenic 30.0%
! TATGATAATGCTGCGTATAT+TGG + chr2.1:56224486-56224505 None:intergenic 30.0%
!! ATCAAGGAAAGTATTGTGTA+AGG + chr2.1:56222923-56222942 None:intergenic 30.0%
!! ATGTTTTTAAGAAATGGGGT+TGG - chr2.1:56224969-56224988 MS.gene00327:intron 30.0%
!! TCAAGGAAAGTATTGTGTAA+GGG + chr2.1:56222922-56222941 None:intergenic 30.0%
!!! ATATGTTCTCAGTTTTAAGG+AGG - chr2.1:56225267-56225286 MS.gene00327:intron 30.0%
!!! GAGTTTTTGGGTTAATCAAT+TGG - chr2.1:56225072-56225091 MS.gene00327:intron 30.0%
!!! GTGGAAGTTTTTTTGAACTT+TGG - chr2.1:56223219-56223238 MS.gene00327:CDS 30.0%
!!! GTTTGATGAAATTCTGGTTT+TGG - chr2.1:56222842-56222861 MS.gene00327:CDS 30.0%
!!! GTTTTTTTGAACTTTGGGTT+TGG - chr2.1:56223225-56223244 MS.gene00327:CDS 30.0%
!!! TTCTAAGATGCTTCCAATTT+TGG - chr2.1:56223180-56223199 MS.gene00327:CDS 30.0%
AATGTCTGAAAAATCAGAGG+TGG - chr2.1:56223275-56223294 MS.gene00327:CDS 35.0%
AATTGAATTGTGGGTATCTG+AGG + chr2.1:56224528-56224547 None:intergenic 35.0%
ACAAATGTTGAGAAATGGTC+AGG + chr2.1:56223894-56223913 None:intergenic 35.0%
ACCCAAAAGTTGAAGTTGAA+GGG + chr2.1:56224895-56224914 None:intergenic 35.0%
AGTTCAAAAAAACTTCCACC+AGG + chr2.1:56223218-56223237 None:intergenic 35.0%
ATTCGGAAGTTGTGAATTTG+TGG + chr2.1:56224316-56224335 None:intergenic 35.0%
CAAAAGCAATCTAGCAATAG+CGG + chr2.1:56224801-56224820 None:intergenic 35.0%
CAAATGCAGTAACTAATGTG+TGG + chr2.1:56223376-56223395 None:intergenic 35.0%
CAAATTCTTAAATGTGGGCT+GGG - chr2.1:56222954-56222973 MS.gene00327:CDS 35.0%
CAACATAATTACACAGCTGA+GGG + chr2.1:56222791-56222810 None:intergenic 35.0%
CACACATTAGTTACTGCATT+TGG - chr2.1:56223374-56223393 MS.gene00327:CDS 35.0%
CAGCCCACATTTAAGAATTT+GGG + chr2.1:56222955-56222974 None:intergenic 35.0%
CCAACACAAATGTTGAGAAA+TGG + chr2.1:56223899-56223918 None:intergenic 35.0%
CCATTTCTCAACATTTGTGT+TGG - chr2.1:56223896-56223915 MS.gene00327:intron 35.0%
CTACTGCTTTAATATGCTCA+TGG - chr2.1:56224016-56224035 MS.gene00327:intron 35.0%
CTGCAATTTCTTGTGATGAA+AGG - chr2.1:56225178-56225197 MS.gene00327:intron 35.0%
CTTAGGAACATTTCCTTAGA+CGG + chr2.1:56225244-56225263 None:intergenic 35.0%
GATGTTATTCGAGCATTTAG+AGG - chr2.1:56225001-56225020 MS.gene00327:intron 35.0%
GTTCAAAAAAACTTCCACCA+GGG + chr2.1:56223217-56223236 None:intergenic 35.0%
TAGAGCAAGAAACTTTGGTT+TGG + chr2.1:56223642-56223661 None:intergenic 35.0%
TATCCATTCTACATCACATG+CGG - chr2.1:56222877-56222896 MS.gene00327:CDS 35.0%
TCAACATAATTACACAGCTG+AGG + chr2.1:56222792-56222811 None:intergenic 35.0%
TCAATTGATCCAAGTATAGC+TGG + chr2.1:56224830-56224849 None:intergenic 35.0%
TCATGAATCTTGAATGGGAT+TGG - chr2.1:56223663-56223682 MS.gene00327:intron 35.0%
TCTGATACCATGATTGCATA+AGG + chr2.1:56223994-56224013 None:intergenic 35.0%
TGATGTGTTTATGAGACTCT+TGG - chr2.1:56223849-56223868 MS.gene00327:intron 35.0%
TGATTTCCTTAGTACTTTGG+AGG - chr2.1:56224847-56224866 MS.gene00327:intron 35.0%
TTCACTTTCTTCACAAACCT+TGG - chr2.1:56224449-56224468 MS.gene00327:intron 35.0%
TTCGGAAGTTGTGAATTTGT+GGG + chr2.1:56224315-56224334 None:intergenic 35.0%
TTCTTGTGATGAAAGGTTTG+AGG - chr2.1:56225185-56225204 MS.gene00327:intron 35.0%
! ACCCTTCAACTTCAACTTTT+GGG - chr2.1:56224891-56224910 MS.gene00327:intron 35.0%
! AGTACTTTGGAGGAAGTTAA+GGG - chr2.1:56224857-56224876 MS.gene00327:intron 35.0%
! CTTTTGACCTTATGCAATCA+TGG - chr2.1:56223984-56224003 MS.gene00327:intron 35.0%
! TAACTTCCTCCAAAGTACTA+AGG + chr2.1:56224856-56224875 None:intergenic 35.0%
! TAGTACTTTGGAGGAAGTTA+AGG - chr2.1:56224856-56224875 MS.gene00327:intron 35.0%
! TTTGGGTTAATCAATTGGGT+TGG - chr2.1:56225077-56225096 MS.gene00327:intron 35.0%
!! AGGGGTTGATAATGATTTGT+GGG + chr2.1:56224273-56224292 None:intergenic 35.0%
!! ATTGGAAGCATCTTAGAACT+TGG + chr2.1:56223178-56223197 None:intergenic 35.0%
!! GATGCTTCCAATTTTGGTAA+TGG - chr2.1:56223186-56223205 MS.gene00327:CDS 35.0%
!! TAGGGGTTGATAATGATTTG+TGG + chr2.1:56224274-56224293 None:intergenic 35.0%
!! TCAATTGGGTTGGAATTCTT+TGG - chr2.1:56225087-56225106 MS.gene00327:intron 35.0%
AGTTGACACTTGATGCATGT+GGG - chr2.1:56223708-56223727 MS.gene00327:intron 40.0%
ATTTCCTTAGACGGAGCAAA+CGG + chr2.1:56225235-56225254 None:intergenic 40.0%
CAGTAACTAATGTGTGGCAT+TGG + chr2.1:56223370-56223389 None:intergenic 40.0%
CCAAATTCTTAAATGTGGGC+TGG - chr2.1:56222953-56222972 MS.gene00327:CDS 40.0%
CCAGCCCACATTTAAGAATT+TGG + chr2.1:56222956-56222975 None:intergenic 40.0%
CTCAACATTTGTGTTGGACA+GGG - chr2.1:56223902-56223921 MS.gene00327:intron 40.0%
GACCCAAAAGTTGAAGTTGA+AGG + chr2.1:56224896-56224915 None:intergenic 40.0%
GCAGAAAAGGATTGTTCCAA+GGG - chr2.1:56225147-56225166 MS.gene00327:intron 40.0%
TAACCGCATGTGATGTAGAA+TGG + chr2.1:56222883-56222902 None:intergenic 40.0%
TAGTTGACACTTGATGCATG+TGG - chr2.1:56223707-56223726 MS.gene00327:intron 40.0%
TCAACATTTGTGTTGGACAG+GGG - chr2.1:56223903-56223922 MS.gene00327:intron 40.0%
TCTCAACATTTGTGTTGGAC+AGG - chr2.1:56223901-56223920 MS.gene00327:intron 40.0%
TCTTACCTCATCAACACCAT+TGG - chr2.1:56224353-56224372 MS.gene00327:intron 40.0%
TGAATCTTGAATGGGATTGG+CGG - chr2.1:56223666-56223685 MS.gene00327:intron 40.0%
TGCAGAAAAGGATTGTTCCA+AGG - chr2.1:56225146-56225165 MS.gene00327:intron 40.0%
TGGAGGAAGTTAAGGGTTTA+GGG - chr2.1:56224864-56224883 MS.gene00327:intron 40.0%
TTCTACATCACATGCGGTTA+TGG - chr2.1:56222883-56222902 MS.gene00327:CDS 40.0%
TTGACAAGACCAGCTATACT+TGG - chr2.1:56224818-56224837 MS.gene00327:intron 40.0%
TTGGAGGAAGTTAAGGGTTT+AGG - chr2.1:56224863-56224882 MS.gene00327:intron 40.0%
! ATTTGGGTTCTGTGCAATCA+AGG + chr2.1:56222939-56222958 None:intergenic 40.0%
! GACCCTTCAACTTCAACTTT+TGG - chr2.1:56224890-56224909 MS.gene00327:intron 40.0%
!! GGGGTTGATAATGATTTGTG+GGG + chr2.1:56224272-56224291 None:intergenic 40.0%
!! GGGTTGATAATGATTTGTGG+GGG + chr2.1:56224271-56224290 None:intergenic 40.0%
!! TTCAACTTTTGGGTCAGCTT+TGG - chr2.1:56224901-56224920 MS.gene00327:intron 40.0%
!!! TTCCAATTTTGGTAATGGCG+TGG - chr2.1:56223191-56223210 MS.gene00327:CDS 40.0%
AATTGCAGAACCAATGCCCT+TGG + chr2.1:56225166-56225185 None:intergenic 45.0%
AGCAAACGGTTCAACTAAGC+TGG + chr2.1:56225221-56225240 None:intergenic 45.0%
CATGTCAATGTTCTCCTTGG+TGG + chr2.1:56225341-56225360 None:intergenic 45.0%
CGTAAATCTTGCATCCACCA+AGG - chr2.1:56225324-56225343 MS.gene00327:CDS 45.0%
CTTCGCTTCACAGTTCTTTG+AGG - chr2.1:56223956-56223975 MS.gene00327:intron 45.0%
GACGGTGAATGAGTCTGATT+CGG + chr2.1:56224333-56224352 None:intergenic 45.0%
GGATGTGGTTGTTGAAATGG+TGG - chr2.1:56222748-56222767 MS.gene00327:CDS 45.0%
TGGCATGTCAATGTTCTCCT+TGG + chr2.1:56225344-56225363 None:intergenic 45.0%
TGTGGGTATCTGAGGAAAGA+AGG + chr2.1:56224520-56224539 None:intergenic 45.0%
TGTTTCACGAGGGAAACCAA+TGG + chr2.1:56224372-56224391 None:intergenic 45.0%
! AAGGATTGTTCCAAGGGCAT+TGG - chr2.1:56225153-56225172 MS.gene00327:intron 45.0%
! CTTTGAGAGCTGTTTCACGA+GGG + chr2.1:56224382-56224401 None:intergenic 45.0%
!! GGAAACCAATGGTGTTGATG+AGG + chr2.1:56224361-56224380 None:intergenic 45.0%
!! GGTGTTGATGAGGTAAGAGA+CGG + chr2.1:56224351-56224370 None:intergenic 45.0%
!!! ATTGCCATTTTGCTCTGAGC+TGG + chr2.1:56224197-56224216 None:intergenic 45.0%
!!! TTGCCATTTTGCTCTGAGCT+GGG + chr2.1:56224196-56224215 None:intergenic 45.0%
ACACTTGATGCATGTGGGCT+AGG - chr2.1:56223713-56223732 MS.gene00327:intron 50.0%
GAGTCCTTCAGAGATGACAC+CGG + chr2.1:56223937-56223956 None:intergenic 50.0%
GGCCACGCCATTACCAAAAT+TGG + chr2.1:56223196-56223215 None:intergenic 50.0%
GTATCTGAGGAAAGAAGGCG+TGG + chr2.1:56224515-56224534 None:intergenic 50.0%
TGAACCGTTTGCTCCGTCTA+AGG - chr2.1:56225228-56225247 MS.gene00327:intron 50.0%
TGACCCAGCTCAGAGCAAAA+TGG - chr2.1:56224190-56224209 MS.gene00327:intron 50.0%
! GAGCGTTGCAGGAGATTTAC+AGG + chr2.1:56223527-56223546 None:intergenic 50.0%
!! ATGGAAAAGGAGAGCGTTGC+AGG + chr2.1:56223538-56223557 None:intergenic 50.0%
!! GCTTTGAGAGCTGTTTCACG+AGG + chr2.1:56224383-56224402 None:intergenic 50.0%
!! GGTTGTTGAAATGGTGGACG+AGG - chr2.1:56222754-56222773 MS.gene00327:CDS 50.0%
TCCTTCAGAGATGACACCGG+CGG + chr2.1:56223934-56223953 None:intergenic 55.0%
!!! TTTTGGTAATGGCGTGGCCC+TGG - chr2.1:56223197-56223216 MS.gene00327:CDS 55.0%
TCAGAGATGACACCGGCGGA+AGG + chr2.1:56223930-56223949 None:intergenic 60.0%
!! TCCGCCGGTGTCATCTCTGA+AGG - chr2.1:56223930-56223949 MS.gene00327:intron 60.0%
TTGGACAGGGGACCTTCCGC+CGG - chr2.1:56223915-56223934 MS.gene00327:intron 65.0%
!! TGGTAATGGCGTGGCCCTGG+TGG - chr2.1:56223200-56223219 MS.gene00327:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56222746 56225375 56222746 ID=MS.gene00327
chr2.1 mRNA 56222746 56225375 56222746 ID=MS.gene00327.t1;Parent=MS.gene00327
chr2.1 exon 56225323 56225375 56225323 ID=MS.gene00327.t1.exon1;Parent=MS.gene00327.t1
chr2.1 CDS 56225323 56225375 56225323 ID=cds.MS.gene00327.t1;Parent=MS.gene00327.t1
chr2.1 exon 56224116 56224198 56224116 ID=MS.gene00327.t1.exon2;Parent=MS.gene00327.t1
chr2.1 CDS 56224116 56224198 56224116 ID=cds.MS.gene00327.t1;Parent=MS.gene00327.t1
chr2.1 exon 56222746 56223542 56222746 ID=MS.gene00327.t1.exon3;Parent=MS.gene00327.t1
chr2.1 CDS 56222746 56223542 56222746 ID=cds.MS.gene00327.t1;Parent=MS.gene00327.t1
Gene Sequence

>MS.gene00327

ATGGATGTGGTTGTTGAAATGGTGGACGAGGCTTCTGATAAACCCTCAGCTGTGTAATTATGTTGAGAATTTCATTGTTTACTGTGACAAATAGGTGTTTGATGAAATTCTGGTTTTGGTTGCTCTTTAATTATCCATTCTACATCACATGCGGTTATGGTTCTTCTGATGAGCCCTTACACAATACTTTCCTTGATTGCACAGAACCCAAATTCTTAAATGTGGGCTGGGTGTTAATTAAGTGTGTGTTTAACAAATGTGTTGCTGAATTAACGTAGATTATTTTTTCTGTTTTGCAGTTGCAAAAATAAAATTAATTTAGATTTTGTTATTAATTTGATTAAAATTGACTGTAGTTGCAAATTGTCTTGATTGGCCTTTAGTGTCATAAATTAGTCTATAGGTTTTTGAGAGTTTTGTGTGTTTTTGCCAAGTTCTAAGATGCTTCCAATTTTGGTAATGGCGTGGCCCTGGTGGAAGTTTTTTTGAACTTTGGGTTTGGTTTAATTTTCATAAGAATGAAAATTATAATGTCTGAAAAATCAGAGGTGGTGACTAGTTGAACCAACTCTACAAGTGTATTTTAGCGAGTTTTGTTTTGCCTTTTCTTTCTTTTTAATACCAATGCCACACATTAGTTACTGCATTTGGTACTAGTTTGTTTTCTTGATATATCTTTGAACATGTACAACATTTTCTATACTACATATTTTCAGAAAATTATGGTATCTTTTTCGTAGATTTTCATTAACGTAAGGAAATGATGTTATTATTTGATCCTGTAAATCTCCTGCAACGCTCTCCTTTTCCATTGTTTTATTGCTAAAAATGCTAGCTCCGCAATGTATACTTAGTTCCTAGTTATTCACAATTTGTTTATTTGATTCATGATACCAAACCAAAGTTTCTTGCTCTATTCATGAATCTTGAATGGGATTGGCGGTTCCCATTTTGAACTTATTAGTTGACACTTGATGCATGTGGGCTAGGATACTGTCTTCATGAATTGTAATTGTATAATGTTGGTGTACACACTGAACTCTGAGAACTTGTACAGAATTGCTACTGCATATTTATATATCTGAATGAGGTAAATCACATCGTGATGTGTTTATGAGACTCTTGGATTACTCTTCTGTTCATTACCTGACCATTTCTCAACATTTGTGTTGGACAGGGGACCTTCCGCCGGTGTCATCTCTGAAGGACTCTTCGCTTCACAGTTCTTTGAGGCAACACTTTTGACCTTATGCAATCATGGTATCAGATGCTACTGCTTTAATATGCTCATGGATAATTGAATGACTGATTAACATAATTAATGAAGCATTTTAACACCCAAGACTATTTTAACCAATAAAGTGCAAAATCAAGAACTATTTTAAAATTTCGATATAACAAAGGACTATTACTCGTAGAGTGACATCACTCTTATTTCATTTCGTGACCCAGCTCAGAGCAAAATGGCAATGCTTCGTTACATTATCTTCAATTACCATCATCATTACAGAGCAATTATTATCCCCCACAAATCATTATCAACCCCTATTTTCCCATTCCAATTTTCCCACAAATTCACAACTTCCGAATCAGACTCATTCACCGTCTCTTACCTCATCAACACCATTGGTTTCCCTCGTGAAACAGCTCTCAAAGCATCAAAACGACTTCGTTTCAACTCTCCACAAAAGCCCAATTCATTTTTCACTTTCTTCACAAACCTTGGTTTTTCAGATTCCAATATACGCAGCATTATCATAAAACAACCACGCCTTCTTTCCTCAGATACCCACAATTCAATTCTTCCAAAGTTTCAATTTTTTCTATCTAAAGGTGCTTCAGCTTCTGAAATCGCGTGTTTGTTAGCAGTGAATCCTAGAATTTTACAGAATAGTTTGGAGAATAGAATAATTCCTCTTTTTGAATTTTATAAGAGGTTTTTTAAATCTGATAAAGCTACAATTTGTTGCATCATTCGAAATTTGATATCTTTGCCTTACAATCTTACTACTGTTAATGTTAAGTTATTGATTGATTATGGAGTTTGTGATTCCGCTATTGCTAGATTGCTTTTGACAAGACCAGCTATACTTGGATCAATTGATTTCCTTAGTACTTTGGAGGAAGTTAAGGGTTTAGGGTTTGACCCTTCAACTTCAACTTTTGGGTCAGCTTTGGTTGCAAAGAAATGTATGACTAAAACACTTTGGGATGAGAAAGTTGATGTTTTTAAGAAATGGGGTTGGTCTGATGAAGATGTTATTCGAGCATTTAGAGGTCGTCCTGAATTGTTGTTGACTTCAATTGATAAGATTAATTTGGTGTTGAGTTTTTGGGTTAATCAATTGGGTTGGAATTCTTTGGCACTTTCTAAAAGGCCACGTATTTTTAGTTACAGTTTGCAGAAAAGGATTGTTCCAAGGGCATTGGTTCTGCAATTTCTTGTGATGAAAGGTTTGAGGAAAAAGAATGCCAGCTTAGTTGAACCGTTTGCTCCGTCTAAGGAAATGTTCCTAAGCAAATATGTTCTCAGTTTTAAGGAGGATTCTGATTATTTATTAAAGCTTTATGAGGAAAACGTAAATCTTGCATCCACCAAGGAGAACATTGACATGCCATTCACCAAATAG
Protein sequence

>MS.gene00327.t1

MDVVVEMVDEASDKPSAVGPSAGVISEGLFASQFFEATLLTLCNHGASASEIACLLAVNPRILQNSLENRIIPLFEFYKRFFKSDKATICCIIRNLISLPYNLTTVNVKLLIDYGVCDSAIARLLLTRPAILGSIDFLSTLEEVKGLGFDPSTSTFGSALVAKKCMTKTLWDEKVDVFKKWGWSDEDVIRAFRGRPELLLTSIDKINLVLSFWVNQLGWNSLALSKRPRIFSYSLQKRIVPRALVLQFLVMKGLRKKNASLVEPFAPSKEMFLSKYVLSFKEDSDYLLKLYEENVNLASTKENIDMPFTK