AlfalfaGEDB | MS.gene073378

M. sativa cultivar XinJiangDaYe / MS.gene073378

KEGG: K15032
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02536.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073378.t1	XP_024633294.1	67.3	392	75	3	15	355	23	412	8.40E-139	503.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073378.t1	A0A072VGL0	67.3	392	75	3	15	355	23	412	6.0e-139	503.4

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene073378.t1	TR	mTERF

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
MS.gene00282	MS.gene073378	PPI
MS.gene00284	MS.gene073378	PPI
MS.gene00286	MS.gene073378	PPI
MS.gene00321	MS.gene073378	PPI
MS.gene00322	MS.gene073378	PPI
MS.gene00324	MS.gene073378	PPI
MS.gene00326	MS.gene073378	PPI
MS.gene00327	MS.gene073378	PPI
MS.gene00381	MS.gene073378	PPI
MS.gene00383	MS.gene073378	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073378.t1	MTR_2g437170	67.347	392	75	3	15	355	23	412	0.0	514
MS.gene073378.t1	MTR_2g437150	63.708	383	86	3	15	346	23	403	9.31e-167	471
MS.gene073378.t1	MTR_2g437160	60.794	403	101	5	1	349	12	411	2.05e-159	453
MS.gene073378.t1	MTR_2g437960	60.976	410	101	5	1	355	1	406	5.26e-158	449
MS.gene073378.t1	MTR_2g437960	60.934	407	100	5	1	352	1	403	1.33e-155	444
MS.gene073378.t1	MTR_2g437940	61.780	382	107	5	1	353	1	372	1.69e-152	434
MS.gene073378.t1	MTR_2g437100	59.383	389	102	4	1	333	7	395	1.44e-148	425
MS.gene073378.t1	MTR_2g437180	57.816	403	103	6	1	346	1	393	6.43e-147	421
MS.gene073378.t1	MTR_2g437120	58.209	402	104	5	1	345	1	395	4.82e-146	419
MS.gene073378.t1	MTR_2g438010	58.201	378	108	3	22	353	48	421	5.06e-145	417

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073378.t1	AT5G07900	28.686	373	202	9	16	330	33	399	1.55e-37	139
MS.gene073378.t1	AT1G21150	26.061	330	193	5	48	328	57	384	2.89e-28	114
MS.gene073378.t1	AT1G21150	25.749	334	197	5	44	328	17	348	2.89e-28	113
MS.gene073378.t1	AT1G21150	26.061	330	193	5	48	328	121	448	8.91e-28	113
MS.gene073378.t1	AT5G64950	26.513	347	198	8	39	330	31	375	1.58e-21	95.1
MS.gene073378.t1	AT1G61980	24.857	350	179	11	48	316	53	399	8.28e-15	75.5
MS.gene073378.t1	AT1G61980	24.857	350	179	11	48	316	53	399	8.28e-15	75.5
MS.gene073378.t1	AT1G61980	24.857	350	179	11	48	316	53	399	8.28e-15	75.5
MS.gene073378.t1	AT1G61970	22.571	350	187	9	48	316	53	399	3.92e-12	67.4
MS.gene073378.t1	AT1G61970	22.571	350	187	9	48	316	53	399	3.92e-12	67.4

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Find 60 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGCTTTGAGAGCAGATTG+AGG	0.549590	2.4:+54558171	None:intergenic
AAAGGATTTGATTAAGTCAT+TGG	0.323129	2.4:-54557695	MS.gene073378:CDS
AACAATTGTTCCAAGGGCAT+TGG	0.458416	2.4:-54557386	MS.gene073378:CDS
AAGAAGGTGAGAACTGAATT+AGG	0.306086	2.4:+54558111	None:intergenic
AAGTTGATGCCCTTAAGAAA+TGG	0.388575	2.4:-54557576	MS.gene073378:CDS
AATTGATATTAGACACAATA+AGG	0.329025	2.4:+54557791	None:intergenic
ACACATTCGGTAGATGGCAT+TGG	0.528449	2.4:+54557178	None:intergenic
ACATCGAATTAAGCAAACAA+TGG	0.642451	2.4:+54557843	None:intergenic
ACCTAAAACTACATTTGTGG+CGG	0.636797	2.4:+54558331	None:intergenic
AGAAACCAAAATTGTTGATG+AGG	0.561539	2.4:+54558196	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGCTACTTCGTAAAATTCT+AGG	+	chr2.4:54557594-54557613	None:intergenic	30.0%
ACATCGAATTAAGCAAACAA+TGG	+	chr2.4:54557679-54557698	None:intergenic	30.0%
AGAAACCAAAATTGTTGATG+AGG	+	chr2.4:54557326-54557345	None:intergenic	30.0%
AGCTACTTCGTAAAATTCTA+GGG	+	chr2.4:54557593-54557612	None:intergenic	30.0%
ATTAGACACAATAAGGTGAT+AGG	+	chr2.4:54557724-54557743	None:intergenic	30.0%
TAACGATGAAACAATATCAG+AGG	+	chr2.4:54557562-54557581	None:intergenic	30.0%
TAGTATCTGATGAGTGAAAT+CGG	+	chr2.4:54557246-54557265	None:intergenic	30.0%
TCATCATCAAAAAAGAACCT+TGG	-	chr2.4:54557466-54557485	MS.gene073378:CDS	30.0%
TTAGACACAATAAGGTGATA+GGG	+	chr2.4:54557723-54557742	None:intergenic	30.0%
TTGAACCATTTCGTTATTCT+GGG	-	chr2.4:54558207-54558226	MS.gene073378:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	54557168	54558373	54557168	ID=MS.gene073378
chr2.4	mRNA	54557168	54558373	54557168	ID=MS.gene073378.t1;Parent=MS.gene073378
chr2.4	exon	54558027	54558373	54558027	ID=MS.gene073378.t1.exon1;Parent=MS.gene073378.t1
chr2.4	CDS	54558027	54558373	54558027	ID=cds.MS.gene073378.t1;Parent=MS.gene073378.t1
chr2.4	exon	54557168	54557888	54557168	ID=MS.gene073378.t1.exon2;Parent=MS.gene073378.t1
chr2.4	CDS	54557168	54557888	54557168	ID=cds.MS.gene073378.t1;Parent=MS.gene073378.t1

Gene Sequence

>MS.gene073378

ATGTTGAAGAAATTGTTTCTCCGCCACAAATGTAGTTTTAGGTCTCATCACAACAACCCTCAACACTTGTTGTTCCCGATTTCACTCATCAGATACTACAACACTACAATAACAACAACATCTGAATCAAACACACACCCATTCGCTGTATCATACCTCATCAACAATTTTGGTTTCTCACCTCAATCTGCTCTCAAAGCTTTCAACAACAAGCAGGTTCGTTTCAATACCCTTGAAAAGCCTAATTCAGTTCTCACCTTCTTAGAAAACCATGGTTTTTCACACGACAACATACGCGTCATCATCAAAAAAGAACCTTGGTTACTTTCATCACAACCCCACAAAAGGTTATTGCTAAAGTTTCAATTTTTTCTATCCAATGGTGCTTCTTCCTCTGATATTGTTTCATCGTTAACTTCAAACCCTAGAATTTTACGAAGTAGCTTGGAGAAACAAATAATCCCTCTTTTTCAATTGTTAAATAGGTTCTTTAAAACCGACAAGGATACCATTGTTTGCTTAATTCGATGTTGGACTGCACTTGCTATTAATCCCTATCACCTTATTGTGTCTAATATCAATTTAATGTCTGATTTTGGAGTTTCTGATTCTGTCATTGCTAGCTCGCTTCAGTCAAGGCCATCTACATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGAGTTTAGGGTTTGATCCTTGGACGAATACTTTTGGAATTGCTTTGGCAGCTAAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCCTTAAGAAATGGGGTTGGTCTAATGAAGCTGTTTTTCAAGCATTTAGGATGCATCCTGGTTTCATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGTTTTTGGGTTAATCAATTGGGTGGGAATTCCTTGGCACTTGCTAAAAGGCCACATATTTTTGATTCAAGTTTGCAGAAAACAATTGTTCCAAGGGCATTGGTTCTGCAATATCTTGTGATGAAAGGTTTGAGGAAAAAGAATGCAAGCTTAGTTGAACCATTTCGTTATTCTGGGAAAATGTTCTTGAGCAAGTTTGTTTTCAGCTTTAAGGAGGAGTCTGATTATCTATTAAAGTTATATGACGAAAAAATGAAACTTGCATACACAAAAGAGAACAATGGCATGCCAATGCCATCTACCGAATGTGTAACTTGTTAA

Protein sequence

>MS.gene073378.t1

MLKKLFLRHKCSFRSHHNNPQHLLFPISLIRYYNTTITTTSESNTHPFAVSYLINNFGFSPQSALKAFNNKQVRFNTLEKPNSVLTFLENHGFSHDNIRVIIKKEPWLLSSQPHKRFFKTDKDTIVCLIRCWTALAINPYHLIVSNINLMSDFGVSDSVIASSLQSRPSTFGSKDLIKSLEEVKSLGFDPWTNTFGIALAAKKSLSKKLWDEKVDALKKWGWSNEAVFQAFRMHPGFMLTSIDKINLVMSFWVNQLGGNSLALAKRPHIFDSSLQKTIVPRALVLQYLVMKGLRKKNASLVEPFRYSGKMFLSKFVFSFKEESDYLLKLYDEKMKLAYTKENNGMPMPSTECVTC