AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004545

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004545.t1 MTR_2g436460 66.404 381 56 4 13 323 1 379 3.02e-161 455
MS.gene004545.t1 MTR_2g437030 40.885 384 151 8 13 323 79 459 7.95e-82 256
MS.gene004545.t1 MTR_2g437040 42.500 360 130 7 35 323 20 373 9.07e-82 253
MS.gene004545.t1 MTR_2g437020 40.710 366 142 8 30 323 11 373 9.21e-78 243
MS.gene004545.t1 MTR_2g437170 40.263 380 146 8 22 321 13 391 1.22e-76 241
MS.gene004545.t1 MTR_2g437940 43.732 343 138 7 28 321 19 355 4.58e-76 238
MS.gene004545.t1 MTR_2g437150 39.628 376 146 8 28 323 19 393 2.16e-75 237
MS.gene004545.t1 MTR_2g436440 41.279 344 131 7 50 323 37 379 3.52e-74 233
MS.gene004545.t1 MTR_2g437160 46.909 275 122 4 51 323 55 307 4.11e-72 226
MS.gene004545.t1 MTR_2g437260 38.329 347 131 5 51 321 28 367 1.51e-71 227
MS.gene004545.t1 MTR_2g437080 36.132 393 159 8 1 323 3 373 1.65e-69 221
MS.gene004545.t1 MTR_2g437130 39.883 341 130 5 54 323 38 374 7.39e-69 220
MS.gene004545.t1 MTR_2g437180 39.402 368 133 9 36 321 22 381 1.67e-68 219
MS.gene004545.t1 MTR_2g076320 38.983 354 140 6 46 323 33 386 1.12e-67 217
MS.gene004545.t1 MTR_2g436400 36.011 361 154 6 39 323 21 380 2.50e-67 216
MS.gene004545.t1 MTR_2g437100 37.867 375 150 8 21 320 29 395 7.08e-67 215
MS.gene004545.t1 MTR_2g437990 39.886 351 136 6 46 321 33 383 5.34e-66 214
MS.gene004545.t1 MTR_2g438010 39.437 355 133 7 46 321 53 404 1.08e-65 213
MS.gene004545.t1 MTR_4g119550 36.337 344 141 6 52 323 46 383 4.93e-65 210
MS.gene004545.t1 MTR_2g437960 39.286 336 124 6 61 320 55 386 5.56e-65 210
MS.gene004545.t1 MTR_2g437120 37.887 388 151 10 19 321 2 384 7.16e-65 210
MS.gene004545.t1 MTR_2g437960 39.286 336 124 6 61 320 55 386 8.67e-65 210
MS.gene004545.t1 MTR_2g437060 34.103 390 183 7 4 323 3 388 4.60e-64 207
MS.gene004545.t1 MTR_2g437160 37.752 347 139 5 51 323 55 398 1.03e-63 207
MS.gene004545.t1 MTR_4g119580 35.435 333 144 5 54 317 39 369 3.54e-59 194
MS.gene004545.t1 MTR_2g019810 37.425 334 136 6 57 320 29 359 5.67e-58 191
MS.gene004545.t1 MTR_2g437200 41.634 257 114 2 99 320 143 398 1.38e-56 188
MS.gene004545.t1 MTR_2g437200 54.783 115 52 0 51 165 24 138 3.57e-33 126
MS.gene004545.t1 MTR_4g120380 35.593 354 152 7 36 317 20 369 2.23e-56 187
MS.gene004545.t1 MTR_2g437240 42.308 260 108 4 99 320 143 398 1.21e-55 186
MS.gene004545.t1 MTR_2g437240 55.856 111 49 0 55 165 28 138 7.66e-32 123
MS.gene004545.t1 MTR_2g438020 47.500 200 99 3 124 321 243 438 4.03e-52 179
MS.gene004545.t1 MTR_2g438020 55.238 105 47 0 61 165 73 177 2.15e-28 114
MS.gene004545.t1 MTR_4g120380 35.224 335 141 7 36 298 20 350 7.50e-51 174
MS.gene004545.t1 MTR_4g120380 26.562 192 115 3 93 284 250 415 2.61e-14 73.6
MS.gene004545.t1 MTR_2g437090 49.342 152 76 1 172 323 11 161 5.98e-47 156
MS.gene004545.t1 MTR_2g019840 34.311 341 155 11 51 323 35 374 8.46e-43 152
MS.gene004545.t1 MTR_3g085240 34.354 294 125 8 19 311 22 248 3.15e-36 131
MS.gene004545.t1 MTR_2g436370 46.763 139 66 2 39 169 20 158 5.79e-36 129
MS.gene004545.t1 MTR_3g450500 29.217 332 158 7 61 317 40 369 9.60e-33 125
MS.gene004545.t1 MTR_4g007490 26.648 349 180 6 44 317 20 367 1.96e-30 119
MS.gene004545.t1 MTR_4g119570 34.247 219 106 5 98 316 47 227 1.20e-28 110
MS.gene004545.t1 MTR_4g049400 42.735 117 61 2 46 156 33 149 4.12e-18 82.4
MS.gene004545.t1 MTR_8g080990 23.774 265 160 5 87 314 109 368 1.73e-15 76.6
MS.gene004545.t1 MTR_5g041630 28.495 186 128 4 94 275 91 275 1.81e-15 76.3
MS.gene004545.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.18e-14 73.6
MS.gene004545.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.51e-14 73.6
MS.gene004545.t1 MTR_8g081000 23.881 268 162 5 87 317 122 384 2.86e-14 73.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004545.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene004545.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene004545.t1 AT1G61980 28.022 364 154 7 61 317 53 415 6.60e-35 131
MS.gene004545.t1 AT1G21150 26.420 352 183 6 40 315 33 384 1.68e-32 124
MS.gene004545.t1 AT1G21150 26.420 352 183 6 40 315 97 448 5.22e-32 124
MS.gene004545.t1 AT5G07900 33.333 216 141 3 105 318 186 400 2.02e-31 122
MS.gene004545.t1 AT5G07900 31.818 132 89 1 38 168 46 177 1.10e-16 80.5
MS.gene004545.t1 AT1G21150 25.294 340 181 5 49 315 9 348 2.63e-31 120
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61970 26.410 390 177 11 35 317 29 415 3.68e-27 110
MS.gene004545.t1 AT1G61990 25.895 363 156 9 61 317 53 408 1.42e-22 97.8
MS.gene004545.t1 AT1G61990 25.895 363 156 9 61 317 53 408 1.42e-22 97.8
MS.gene004545.t1 AT1G62120 42.105 114 66 0 44 157 40 153 4.11e-22 96.7
MS.gene004545.t1 AT1G62120 29.412 170 83 4 171 304 238 406 1.14e-11 65.9
MS.gene004545.t1 AT3G46950 38.621 145 77 2 25 164 19 156 7.65e-22 95.9
MS.gene004545.t1 AT1G62085 41.346 104 61 0 61 164 56 159 1.33e-19 89.4
MS.gene004545.t1 AT1G62085 22.822 241 148 5 67 304 235 440 4.84e-11 63.9
MS.gene004545.t1 AT1G62085 41.346 104 61 0 61 164 56 159 1.33e-19 89.4
MS.gene004545.t1 AT1G62085 22.822 241 148 5 67 304 235 440 4.84e-11 63.9
MS.gene004545.t1 AT1G61960 39.450 109 66 0 61 169 53 161 7.17e-19 87.4
MS.gene004545.t1 AT1G61960 22.989 261 161 5 67 322 231 456 1.06e-13 72.0
MS.gene004545.t1 AT5G23930 39.316 117 71 0 41 157 36 152 1.58e-18 86.3
MS.gene004545.t1 AT1G62110 39.167 120 72 1 39 157 30 149 3.02e-18 85.5
MS.gene004545.t1 AT1G62150 39.423 104 63 0 61 164 59 162 2.13e-17 82.8
MS.gene004545.t1 AT1G62150 29.208 202 131 7 126 323 263 456 1.57e-12 68.6
MS.gene004545.t1 AT5G64950 28.986 207 136 5 119 317 172 375 2.90e-15 76.3
MS.gene004545.t1 AT1G56380 29.187 209 116 3 57 234 24 231 2.66e-14 73.6
MS.gene004545.t1 AT1G56380 29.187 209 116 3 57 234 13 220 2.93e-14 73.2
MS.gene004545.t1 AT1G62010 30.769 169 80 4 171 303 216 383 3.62e-14 73.2

Find 69 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAATAAGGTGATGAGATTT+TGG 0.153456 2.1:+56931758 MS.gene004545:CDS
ACAAGAAGCCCTAGAATTCT+TGG 0.255383 2.1:+56931307 MS.gene004545:CDS
AGACTTGAACCAAGAATTCT+AGG 0.272133 2.1:-56931316 None:intergenic
TTCTGATGAGAAACCGAAAT+TGG 0.280006 2.1:-56931062 None:intergenic
TCATATCTCATTACCAATTT+CGG 0.283798 2.1:+56931049 MS.gene004545:CDS
TGATTTCGACATGGCCCTTT+TGG 0.313754 2.1:-56931626 None:intergenic
AAAGAGGGGTTTGAAGCTTA+AGG 0.317138 2.1:-56930906 None:intergenic
TTTCGTATAGTTTGGAGAAT+AGG 0.328948 2.1:+56931833 MS.gene004545:CDS
AAAGAGTAGAAATGAACTTT+GGG 0.332780 2.1:-56930967 None:intergenic
GTATTGTTGTGGTTCGATTT+AGG 0.332858 2.1:-56930934 None:intergenic
ATTGAATTGTCTACTCCATT+TGG 0.352685 2.1:+56931916 MS.gene004545:CDS
TTCGTATAGTTTGGAGAATA+GGG 0.359509 2.1:+56931834 MS.gene004545:CDS
AAGTTGTTGTCTTTAAGAAA+TGG 0.363837 2.1:+56931662 MS.gene004545:CDS
ATCCACGGAGAAAATTAATA+AGG 0.367860 2.1:+56931744 MS.gene004545:CDS
TTGGGTCAATGAGTTGGGTT+GGG 0.371128 2.1:+56931777 MS.gene004545:CDS
AGTTGTTGTCTTTAAGAAAT+GGG 0.373623 2.1:+56931663 MS.gene004545:CDS
TTGGATTTGGTTAAGGAAAT+GGG 0.379428 2.1:+56931559 MS.gene004545:intron
TTCAGAGGCACAACAAATCT+TGG 0.379614 2.1:+56930864 MS.gene004545:CDS
GAAAGAGTAGAAATGAACTT+TGG 0.387896 2.1:-56930968 None:intergenic
AAATGGTTCTTGAAGCGTTT+AGG 0.395855 2.1:+56931698 MS.gene004545:CDS
TGAGTTGGGTTGGGACTCTT+CGG 0.410843 2.1:+56931786 MS.gene004545:CDS
CTTCTTACCGATTCAAATGT+AGG 0.416369 2.1:-56931355 None:intergenic
GGGGTCACATTTAAGAATGT+TGG 0.425752 2.1:-56931209 None:intergenic
TCAAGCATTGTTAGCCAAAA+GGG 0.433648 2.1:+56931612 MS.gene004545:CDS
GTCAAGCATTGTTAGCCAAA+AGG 0.438054 2.1:+56931611 MS.gene004545:CDS
TTAAATGTGACCCCTACAAA+AGG 0.439736 2.1:+56931218 MS.gene004545:CDS
TGAGATATGAAACTGTGAAC+TGG 0.448214 2.1:-56931036 None:intergenic
AGGAGGAGTGGTTCAAAGAG+AGG 0.463557 2.1:-56930994 None:intergenic
ATGAACTTTGGGGTTGGAAA+GGG 0.467912 2.1:-56930956 None:intergenic
GGGTCACATTTAAGAATGTT+GGG 0.468503 2.1:-56931208 None:intergenic
GGTTTGTTGTGAAGTGGTTG+AGG 0.469829 2.1:-56931014 None:intergenic
ACTCTTCGGCTCTTGTCAAA+AGG 0.469912 2.1:+56931800 MS.gene004545:CDS
TGCTTCAAATATTGATATTG+TGG 0.478150 2.1:+56931276 MS.gene004545:CDS
TCGATTTAGGTTGAGAAAGA+GGG 0.479858 2.1:-56930921 None:intergenic
TTCTTACCGATTCAAATGTA+GGG 0.480837 2.1:-56931354 None:intergenic
TTCTTGAAGCGTTTAGGAAG+CGG 0.487125 2.1:+56931704 MS.gene004545:CDS
TTTGGGTCAATGAGTTGGGT+TGG 0.489140 2.1:+56931776 MS.gene004545:CDS
GGCCATGTCGAAATCACGTT+GGG 0.495859 2.1:+56931633 MS.gene004545:CDS
AAAACAGCGATTACTGAGTC+GGG 0.496874 2.1:-56931133 None:intergenic
TTCGATTTAGGTTGAGAAAG+AGG 0.496950 2.1:-56930922 None:intergenic
ATGGGGTTGGTCTGATGAAA+TGG 0.499033 2.1:+56931681 MS.gene004545:CDS
TATAATCCCTACATTTGAAT+CGG 0.504738 2.1:+56931348 MS.gene004545:CDS
GGAGGAGTGGTTCAAAGAGA+GGG 0.505452 2.1:-56930993 None:intergenic
GTAGAAATGAACTTTGGGGT+TGG 0.510973 2.1:-56930962 None:intergenic
GTTGTTGTCTTTAAGAAATG+GGG 0.513781 2.1:+56931664 MS.gene004545:CDS
AATGAACTTTGGGGTTGGAA+AGG 0.516771 2.1:-56930957 None:intergenic
TAAATGTGACCCCTACAAAA+GGG 0.524191 2.1:+56931219 MS.gene004545:CDS
TGCGAAACAACACAAGCCCT+TGG 0.528307 2.1:-56931862 None:intergenic
TTCGGCTCTTGTCAAAAGGC+CGG 0.528513 2.1:+56931804 MS.gene004545:CDS
GAAAACAGCGATTACTGAGT+CGG 0.530805 2.1:-56931134 None:intergenic
GGTTGGAAAGGGTATTGTTG+TGG 0.539751 2.1:-56930945 None:intergenic
TGGAGAATAGGGTCATTCCA+AGG 0.540810 2.1:+56931845 MS.gene004545:CDS
AAGCTTAAGGATTTCAACAT+CGG 0.550353 2.1:-56930893 None:intergenic
TTGTTGTGAAGTGGTTGAGG+AGG 0.551983 2.1:-56931011 None:intergenic
TTTCAATCTTGATGTTTCAG+AGG 0.555969 2.1:+56930849 None:intergenic
CGATTTAGGTTGAGAAAGAG+GGG 0.559408 2.1:-56930920 None:intergenic
TTGTCTTTAAGAAATGGGGT+TGG 0.562623 2.1:+56931668 MS.gene004545:CDS
GTGAAGTGGTTGAGGAGGAG+TGG 0.563267 2.1:-56931006 None:intergenic
ATTGTGTATGTTAGCATCCA+CGG 0.574891 2.1:+56931729 MS.gene004545:CDS
GGAGAATAGGGTCATTCCAA+GGG 0.578298 2.1:+56931846 MS.gene004545:CDS
GACTTGAACCAAGAATTCTA+GGG 0.579810 2.1:-56931315 None:intergenic
GGCTCTTGTCAAAAGGCCGG+AGG 0.586864 2.1:+56931807 MS.gene004545:CDS
GTTTCGCATTTGATCTCCAG+AGG 0.592319 2.1:+56931877 MS.gene004545:CDS
GAACTGGGTTTGTTGTGAAG+TGG 0.592348 2.1:-56931020 None:intergenic
GAGATATGAAACTGTGAACT+GGG 0.635008 2.1:-56931035 None:intergenic
AAGAGTAGAAATGAACTTTG+GGG 0.644547 2.1:-56930966 None:intergenic
GGGCCATGTCGAAATCACGT+TGG 0.650039 2.1:+56931632 MS.gene004545:CDS
ATCCCAACGTGATTTCGACA+TGG 0.666340 2.1:-56931635 None:intergenic
GTGGATGCTAACATACACAA+TGG 0.700210 2.1:-56931727 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAATTTTTAACTTCAAA+AGG + chr2.1:56931253-56931272 MS.gene004545:CDS 15.0%
!! GAAGTTAAAAATTCAAACTT+TGG - chr2.1:56931250-56931269 None:intergenic 20.0%
!! TCATAAGTCAATAATATAGA+CGG - chr2.1:56931526-56931545 None:intergenic 20.0%
!!! CTTCATTGTAAACATTTTTT+TGG + chr2.1:56931415-56931434 MS.gene004545:intron 20.0%
!!! TATTATTGACTTATGATATG+AGG + chr2.1:56931530-56931549 MS.gene004545:intron 20.0%
! AAAGAGTAGAAATGAACTTT+GGG - chr2.1:56930970-56930989 None:intergenic 25.0%
! AAAGCTATACCTAGAAAAAA+TGG + chr2.1:56932005-56932024 MS.gene004545:CDS 25.0%
! AAGCTATACCTAGAAAAAAT+GGG + chr2.1:56932006-56932025 MS.gene004545:CDS 25.0%
! TATAATCCCTACATTTGAAT+CGG + chr2.1:56931348-56931367 MS.gene004545:CDS 25.0%
! TCAATAACTTTCTCATTAGA+AGG - chr2.1:56931388-56931407 None:intergenic 25.0%
! TCATATCTCATTACCAATTT+CGG + chr2.1:56931049-56931068 MS.gene004545:CDS 25.0%
! TGCTTCAAATATTGATATTG+TGG + chr2.1:56931276-56931295 MS.gene004545:CDS 25.0%
!! AAGTTGTTGTCTTTAAGAAA+TGG + chr2.1:56931662-56931681 MS.gene004545:CDS 25.0%
!! ACATTTTCATTAACACCAAA+TGG - chr2.1:56931934-56931953 None:intergenic 25.0%
!! AGTTGTTGTCTTTAAGAAAT+GGG + chr2.1:56931663-56931682 MS.gene004545:CDS 25.0%
!! GTTAATATGTTACTTGATGA+TGG + chr2.1:56931460-56931479 MS.gene004545:intron 25.0%
!! TAATAAGGTGATGAGATTTT+GGG + chr2.1:56931759-56931778 MS.gene004545:CDS 25.0%
!! TTAATAAGGTGATGAGATTT+TGG + chr2.1:56931758-56931777 MS.gene004545:CDS 25.0%
AAACTATACGAAAAAACCTC+CGG - chr2.1:56931826-56931845 None:intergenic 30.0%
AAGAGTAGAAATGAACTTTG+GGG - chr2.1:56930969-56930988 None:intergenic 30.0%
AAGCTTAAGGATTTCAACAT+CGG - chr2.1:56930896-56930915 None:intergenic 30.0%
ATCCACGGAGAAAATTAATA+AGG + chr2.1:56931744-56931763 MS.gene004545:CDS 30.0%
ATTGAATTGTCTACTCCATT+TGG + chr2.1:56931916-56931935 MS.gene004545:CDS 30.0%
GAAAGAGTAGAAATGAACTT+TGG - chr2.1:56930971-56930990 None:intergenic 30.0%
TAACTTTCTCATTAGAAGGA+AGG - chr2.1:56931384-56931403 None:intergenic 30.0%
TGACAAAAATTCACATTGGT+AGG - chr2.1:56931595-56931614 None:intergenic 30.0%
TGCTTGACAAAAATTCACAT+TGG - chr2.1:56931599-56931618 None:intergenic 30.0%
TTATGATATGAGGAATGCTT+TGG + chr2.1:56931540-56931559 MS.gene004545:intron 30.0%
TTCGTATAGTTTGGAGAATA+GGG + chr2.1:56931834-56931853 MS.gene004545:CDS 30.0%
TTTCGTATAGTTTGGAGAAT+AGG + chr2.1:56931833-56931852 MS.gene004545:CDS 30.0%
! AAAGTATGTGCAGTGTTTTA+AGG + chr2.1:56931963-56931982 MS.gene004545:CDS 30.0%
! ATTCTTTTCTATCAAACCTC+TGG - chr2.1:56931896-56931915 None:intergenic 30.0%
! GTTGTTGTCTTTAAGAAATG+GGG + chr2.1:56931664-56931683 MS.gene004545:CDS 30.0%
! TTGGATTTGGTTAAGGAAAT+GGG + chr2.1:56931559-56931578 MS.gene004545:intron 30.0%
! TTTGGATTTGGTTAAGGAAA+TGG + chr2.1:56931558-56931577 MS.gene004545:intron 30.0%
!! TTCTTACCGATTCAAATGTA+GGG - chr2.1:56931357-56931376 None:intergenic 30.0%
AGACTTGAACCAAGAATTCT+AGG - chr2.1:56931319-56931338 None:intergenic 35.0%
ATTGTGTATGTTAGCATCCA+CGG + chr2.1:56931729-56931748 MS.gene004545:CDS 35.0%
CTACATTTGAATCGGTAAGA+AGG + chr2.1:56931356-56931375 MS.gene004545:intron 35.0%
GACTTGAACCAAGAATTCTA+GGG - chr2.1:56931318-56931337 None:intergenic 35.0%
GAGATATGAAACTGTGAACT+GGG - chr2.1:56931038-56931057 None:intergenic 35.0%
GGGTCACATTTAAGAATGTT+GGG - chr2.1:56931211-56931230 None:intergenic 35.0%
GTATTGTTGTGGTTCGATTT+AGG - chr2.1:56930937-56930956 None:intergenic 35.0%
TAAATGTGACCCCTACAAAA+GGG + chr2.1:56931219-56931238 MS.gene004545:CDS 35.0%
TGAGATATGAAACTGTGAAC+TGG - chr2.1:56931039-56931058 None:intergenic 35.0%
TTAAATGTGACCCCTACAAA+AGG + chr2.1:56931218-56931237 MS.gene004545:CDS 35.0%
TTCTGATGAGAAACCGAAAT+TGG - chr2.1:56931065-56931084 None:intergenic 35.0%
! AAATGGTTCTTGAAGCGTTT+AGG + chr2.1:56931698-56931717 MS.gene004545:CDS 35.0%
! CACCTTATTAATTTTCTCCG+TGG - chr2.1:56931749-56931768 None:intergenic 35.0%
! GAATGCTTTGGATTTGGTTA+AGG + chr2.1:56931552-56931571 MS.gene004545:intron 35.0%
! TATGAGGAATGCTTTGGATT+TGG + chr2.1:56931546-56931565 MS.gene004545:intron 35.0%
! TCAAGCATTGTTAGCCAAAA+GGG + chr2.1:56931612-56931631 MS.gene004545:CDS 35.0%
! TTGTCTTTAAGAAATGGGGT+TGG + chr2.1:56931668-56931687 MS.gene004545:CDS 35.0%
! TTTGGCAAAACCCTTTTGTA+GGG - chr2.1:56931232-56931251 None:intergenic 35.0%
!! AGATTTTGGGTCAATGAGTT+GGG + chr2.1:56931772-56931791 MS.gene004545:CDS 35.0%
!! ATCGCTGTTTTCAGAAACTA+CGG + chr2.1:56931145-56931164 MS.gene004545:CDS 35.0%
!! CTTCTTACCGATTCAAATGT+AGG - chr2.1:56931358-56931377 None:intergenic 35.0%
!! TCGATTTAGGTTGAGAAAGA+GGG - chr2.1:56930924-56930943 None:intergenic 35.0%
!! TTCGATTTAGGTTGAGAAAG+AGG - chr2.1:56930925-56930944 None:intergenic 35.0%
!!! GGAGGTTTTTTCGTATAGTT+TGG + chr2.1:56931825-56931844 MS.gene004545:CDS 35.0%
AAAACAGCGATTACTGAGTC+GGG - chr2.1:56931136-56931155 None:intergenic 40.0%
ACAAGAAGCCCTAGAATTCT+TGG + chr2.1:56931307-56931326 MS.gene004545:CDS 40.0%
GAAAACAGCGATTACTGAGT+CGG - chr2.1:56931137-56931156 None:intergenic 40.0%
GGGGTCACATTTAAGAATGT+TGG - chr2.1:56931212-56931231 None:intergenic 40.0%
GTGGATGCTAACATACACAA+TGG - chr2.1:56931730-56931749 None:intergenic 40.0%
! AAAGAGGGGTTTGAAGCTTA+AGG - chr2.1:56930909-56930928 None:intergenic 40.0%
! CTTTGGCAAAACCCTTTTGT+AGG - chr2.1:56931233-56931252 None:intergenic 40.0%
! GATTACTGAGTCGGGTTTTT+GGG - chr2.1:56931128-56931147 None:intergenic 40.0%
! GTCAAGCATTGTTAGCCAAA+AGG + chr2.1:56931611-56931630 MS.gene004545:CDS 40.0%
! TTCAGAGGCACAACAAATCT+TGG + chr2.1:56930864-56930883 MS.gene004545:CDS 40.0%
! TTCTTGAAGCGTTTAGGAAG+CGG + chr2.1:56931704-56931723 MS.gene004545:CDS 40.0%
! TTGGCAAAACCCTTTTGTAG+GGG - chr2.1:56931231-56931250 None:intergenic 40.0%
!! AATGAACTTTGGGGTTGGAA+AGG - chr2.1:56930960-56930979 None:intergenic 40.0%
!! ATGAACTTTGGGGTTGGAAA+GGG - chr2.1:56930959-56930978 None:intergenic 40.0%
!! CGATTTAGGTTGAGAAAGAG+GGG - chr2.1:56930923-56930942 None:intergenic 40.0%
!! GAGATTTTGGGTCAATGAGT+TGG + chr2.1:56931771-56931790 MS.gene004545:CDS 40.0%
!! GTAGAAATGAACTTTGGGGT+TGG - chr2.1:56930965-56930984 None:intergenic 40.0%
!!! GTATGTGCAGTGTTTTAAGG+AGG + chr2.1:56931966-56931985 MS.gene004545:CDS 40.0%
ACTCTTCGGCTCTTGTCAAA+AGG + chr2.1:56931800-56931819 MS.gene004545:CDS 45.0%
ATCCCAACGTGATTTCGACA+TGG - chr2.1:56931638-56931657 None:intergenic 45.0%
GGAGAATAGGGTCATTCCAA+GGG + chr2.1:56931846-56931865 MS.gene004545:CDS 45.0%
GTTTCGCATTTGATCTCCAG+AGG + chr2.1:56931877-56931896 MS.gene004545:CDS 45.0%
TGGAGAATAGGGTCATTCCA+AGG + chr2.1:56931845-56931864 MS.gene004545:CDS 45.0%
TTGTTGTGAAGTGGTTGAGG+AGG - chr2.1:56931014-56931033 None:intergenic 45.0%
! CGATTACTGAGTCGGGTTTT+TGG - chr2.1:56931129-56931148 None:intergenic 45.0%
! TGATTTCGACATGGCCCTTT+TGG - chr2.1:56931629-56931648 None:intergenic 45.0%
! TTGGGTCAATGAGTTGGGTT+GGG + chr2.1:56931777-56931796 MS.gene004545:CDS 45.0%
! TTTGGGTCAATGAGTTGGGT+TGG + chr2.1:56931776-56931795 MS.gene004545:CDS 45.0%
!! ATGGGGTTGGTCTGATGAAA+TGG + chr2.1:56931681-56931700 MS.gene004545:CDS 45.0%
!! GAACTGGGTTTGTTGTGAAG+TGG - chr2.1:56931023-56931042 None:intergenic 45.0%
!! GGTTGGAAAGGGTATTGTTG+TGG - chr2.1:56930948-56930967 None:intergenic 45.0%
!! GGTTTGTTGTGAAGTGGTTG+AGG - chr2.1:56931017-56931036 None:intergenic 45.0%
AGGAGGAGTGGTTCAAAGAG+AGG - chr2.1:56930997-56931016 None:intergenic 50.0%
GGAGGAGTGGTTCAAAGAGA+GGG - chr2.1:56930996-56931015 None:intergenic 50.0%
GGCCATGTCGAAATCACGTT+GGG + chr2.1:56931633-56931652 MS.gene004545:CDS 50.0%
TGCGAAACAACACAAGCCCT+TGG - chr2.1:56931865-56931884 None:intergenic 50.0%
TTCGGCTCTTGTCAAAAGGC+CGG + chr2.1:56931804-56931823 MS.gene004545:CDS 50.0%
!! TGAGTTGGGTTGGGACTCTT+CGG + chr2.1:56931786-56931805 MS.gene004545:CDS 50.0%
GGGCCATGTCGAAATCACGT+TGG + chr2.1:56931632-56931651 MS.gene004545:CDS 55.0%
GTGAAGTGGTTGAGGAGGAG+TGG - chr2.1:56931009-56931028 None:intergenic 55.0%
GGCTCTTGTCAAAAGGCCGG+AGG + chr2.1:56931807-56931826 MS.gene004545:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56930860 56932035 56930860 ID=MS.gene004545
chr2.1 mRNA 56930860 56932035 56930860 ID=MS.gene004545.t1;Parent=MS.gene004545
chr2.1 exon 56930860 56931369 56930860 ID=MS.gene004545.t1.exon1;Parent=MS.gene004545.t1
chr2.1 CDS 56930860 56931369 56930860 ID=cds.MS.gene004545.t1;Parent=MS.gene004545.t1
chr2.1 exon 56931574 56932035 56931574 ID=MS.gene004545.t1.exon2;Parent=MS.gene004545.t1
chr2.1 CDS 56931574 56932035 56931574 ID=cds.MS.gene004545.t1;Parent=MS.gene004545.t1
Gene Sequence

>MS.gene004545

ATGTTTCAGAGGCACAACAAATCTTGGAACTCTCCGATGTTGAAATCCTTAAGCTTCAAACCCCTCTTTCTCAACCTAAATCGAACCACAACAATACCCTTTCCAACCCCAAAGTTCATTTCTACTCTTTCAACCCTCTCTTTGAACCACTCCTCCTCAACCACTTCACAACAAACCCAGTTCACAGTTTCATATCTCATTACCAATTTCGGTTTCTCATCAGAAACAGCTTCAAAAGCTTCAAAACTAGTTCAATTCGACACTTCCCAAAAACCCGACTCAGTAATCGCTGTTTTCAGAAACTACGGTTTCTCTGATTCTCAGATAAAAAACATCATTAGACAAGCACCCAACATTCTTAAATGTGACCCCTACAAAAGGGTTTTGCCAAAGTTTGAATTTTTAACTTCAAAAGGTGCTTCAAATATTGATATTGTGGAAATAGTTACAAGAAGCCCTAGAATTCTTGGTTCAAGTCTTGAGAATAGTATAATCCCTACATTTGAATCGGTAAGAAGGTTCCTTCCTTCTAATGAGAAAGTTATTGAACGTATACTTCATTGTAAACATTTTTTTGGTCATTTTCATTTTATTAGAAATGTTAATATGTTACTTGATGATGGAGTCATTCACTCAAATATCACTTTTTTATTGCTTAGAAGACCGTCTATATTATTGACTTATGATATGAGGAATGCTTTGGATTTGGTTAAGGAAATGGGTTTTGATGATCCTACCAATGTGAATTTTTGTCAAGCATTGTTAGCCAAAAGGGCCATGTCGAAATCACGTTGGGATGCGAAAGTTGTTGTCTTTAAGAAATGGGGTTGGTCTGATGAAATGGTTCTTGAAGCGTTTAGGAAGCGGCCATTGTGTATGTTAGCATCCACGGAGAAAATTAATAAGGTGATGAGATTTTGGGTCAATGAGTTGGGTTGGGACTCTTCGGCTCTTGTCAAAAGGCCGGAGGTTTTTTCGTATAGTTTGGAGAATAGGGTCATTCCAAGGGCTTGTGTTGTTTCGCATTTGATCTCCAGAGGTTTGATAGAAAAGAATATTGAATTGTCTACTCCATTTGGTGTTAATGAAAATGTGTTTCTTGAAAAGTATGTGCAGTGTTTTAAGGAGGAAAGAGATGATTTGTTAAAGCTATACCTAGAAAAAATGGGTGTCTGA
Protein sequence

>MS.gene004545.t1

MFQRHNKSWNSPMLKSLSFKPLFLNLNRTTTIPFPTPKFISTLSTLSLNHSSSTTSQQTQFTVSYLITNFGFSSETASKASKLVQFDTSQKPDSVIAVFRNYGFSDSQIKNIIRQAPNILKCDPYKRVLPKFEFLTSKGASNIDIVEIVTRSPRILGSSLENSIIPTFESEMGFDDPTNVNFCQALLAKRAMSKSRWDAKVVVFKKWGWSDEMVLEAFRKRPLCMLASTEKINKVMRFWVNELGWDSSALVKRPEVFSYSLENRVIPRACVVSHLISRGLIEKNIELSTPFGVNENVFLEKYVQCFKEERDDLLKLYLEKMGV