AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00322

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00322.t1 MTR_2g437040 93.473 383 25 0 1 383 1 383 0.0 720
MS.gene00322.t1 MTR_2g436440 65.464 388 128 2 1 382 1 388 0.0 510
MS.gene00322.t1 MTR_2g437060 66.410 390 119 2 3 380 6 395 0.0 508
MS.gene00322.t1 MTR_2g437080 63.874 382 138 0 1 382 1 382 9.82e-178 499
MS.gene00322.t1 MTR_2g437020 64.516 372 126 3 14 380 10 380 2.74e-169 478
MS.gene00322.t1 MTR_2g437030 62.660 391 136 2 1 382 79 468 2.37e-168 479
MS.gene00322.t1 MTR_2g436400 62.097 372 139 1 5 374 10 381 1.68e-164 466
MS.gene00322.t1 MTR_4g119550 50.435 345 168 2 36 379 47 389 5.89e-124 363
MS.gene00322.t1 MTR_2g437260 49.162 358 176 3 24 377 18 373 2.85e-121 356
MS.gene00322.t1 MTR_2g437200 50.266 376 147 4 33 370 25 398 1.10e-120 355
MS.gene00322.t1 MTR_2g437130 50.139 361 176 2 22 382 27 383 4.01e-120 353
MS.gene00322.t1 MTR_2g437990 48.711 388 192 3 2 382 7 394 7.39e-120 354
MS.gene00322.t1 MTR_2g437240 49.096 387 157 3 35 383 27 411 2.71e-119 352
MS.gene00322.t1 MTR_2g437120 50.847 354 170 2 32 382 43 395 5.93e-119 351
MS.gene00322.t1 MTR_2g438010 47.815 389 195 3 2 382 27 415 6.82e-118 349
MS.gene00322.t1 MTR_2g437960 50.872 344 166 1 42 382 55 398 5.14e-116 343
MS.gene00322.t1 MTR_2g437960 50.872 344 166 1 42 382 55 398 1.03e-115 343
MS.gene00322.t1 MTR_2g437170 50.278 360 170 3 32 382 43 402 2.30e-115 342
MS.gene00322.t1 MTR_2g436460 48.031 381 175 6 13 373 2 379 4.71e-114 337
MS.gene00322.t1 MTR_2g076320 47.301 389 197 3 2 382 7 395 7.55e-114 338
MS.gene00322.t1 MTR_2g437160 48.485 363 185 1 22 382 45 407 1.35e-113 337
MS.gene00322.t1 MTR_2g437150 48.774 367 178 4 26 382 36 402 2.54e-112 334
MS.gene00322.t1 MTR_2g437180 48.857 350 174 2 36 382 45 392 1.57e-110 329
MS.gene00322.t1 MTR_2g437100 50.898 334 159 2 42 370 62 395 2.16e-107 322
MS.gene00322.t1 MTR_4g120380 46.791 374 189 6 1 368 1 370 4.81e-105 314
MS.gene00322.t1 MTR_2g438020 45.503 378 164 4 39 377 70 444 8.55e-105 318
MS.gene00322.t1 MTR_4g119580 46.471 340 178 2 31 368 33 370 3.38e-104 312
MS.gene00322.t1 MTR_2g437940 46.927 358 162 3 25 382 37 366 3.09e-101 305
MS.gene00322.t1 MTR_4g120380 46.893 354 178 6 1 348 1 350 1.44e-97 297
MS.gene00322.t1 MTR_2g019810 45.970 335 169 7 41 370 32 359 7.98e-95 288
MS.gene00322.t1 MTR_2g019840 42.105 380 204 6 4 374 3 375 3.50e-93 284
MS.gene00322.t1 MTR_2g436370 62.121 198 63 3 1 189 1 195 8.69e-73 226
MS.gene00322.t1 MTR_2g437090 61.446 166 64 0 215 380 3 168 8.77e-72 222
MS.gene00322.t1 MTR_2g437160 49.754 203 99 1 183 382 114 316 4.58e-63 205
MS.gene00322.t1 MTR_3g450500 32.353 374 234 5 6 368 5 370 6.19e-60 198
MS.gene00322.t1 MTR_4g119570 37.751 249 122 1 120 368 14 229 1.22e-52 175
MS.gene00322.t1 MTR_4g007490 31.988 347 227 5 28 368 25 368 7.51e-50 172
MS.gene00322.t1 MTR_8g080990 31.757 296 199 2 72 364 73 368 4.00e-41 149
MS.gene00322.t1 MTR_8g081000 26.349 315 228 3 71 381 85 399 1.56e-31 123
MS.gene00322.t1 MTR_8g081000 26.578 301 218 2 71 368 85 385 2.25e-31 123
MS.gene00322.t1 MTR_8g081000 26.578 301 218 2 71 368 85 385 3.02e-31 123
MS.gene00322.t1 MTR_4g049400 48.214 112 54 1 32 139 38 149 4.30e-24 99.8
MS.gene00322.t1 MTR_2g436380 43.119 109 55 3 217 325 5 106 2.41e-20 90.5
MS.gene00322.t1 MTR_3g085240 37.580 157 85 4 10 163 25 171 2.54e-19 87.0
MS.gene00322.t1 MTR_3g085240 50.847 59 29 0 272 330 176 234 1.31e-13 70.5
MS.gene00322.t1 MTR_8g012210 22.414 290 214 7 86 368 63 348 5.45e-13 70.1
MS.gene00322.t1 MTR_4g119615 56.000 50 22 0 312 361 26 75 1.23e-12 63.9
MS.gene00322.t1 MTR_3g092710 23.684 266 181 6 95 348 236 491 1.26e-12 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00322.t1 AT5G07900 33.934 333 212 4 41 367 69 399 1.75e-61 203
MS.gene00322.t1 AT1G21150 31.627 332 217 6 41 365 56 384 6.01e-46 162
MS.gene00322.t1 AT1G21150 30.321 343 229 6 30 365 9 348 7.14e-46 161
MS.gene00322.t1 AT1G21150 31.627 332 217 6 41 365 120 448 2.08e-45 162
MS.gene00322.t1 AT1G61980 30.081 369 211 8 41 367 52 415 2.36e-43 156
MS.gene00322.t1 AT1G61980 30.081 369 211 8 41 367 52 415 2.36e-43 156
MS.gene00322.t1 AT1G61980 30.081 369 211 8 41 367 52 415 2.36e-43 156
MS.gene00322.t1 AT1G62085 29.024 410 206 10 27 353 32 439 1.38e-39 147
MS.gene00322.t1 AT1G62085 29.024 410 206 10 27 353 32 439 1.38e-39 147
MS.gene00322.t1 AT3G46950 27.901 405 213 9 22 353 32 430 6.56e-39 144
MS.gene00322.t1 AT1G62120 29.219 397 227 9 5 355 19 407 6.27e-38 142
MS.gene00322.t1 AT1G61990 27.981 411 239 7 5 367 7 408 8.34e-38 141
MS.gene00322.t1 AT1G61990 27.981 411 239 7 5 367 7 408 8.34e-38 141
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT1G61970 28.571 406 220 10 5 353 7 399 3.48e-37 139
MS.gene00322.t1 AT5G64950 30.682 352 212 10 30 359 25 366 1.23e-33 129
MS.gene00322.t1 AT1G61960 26.044 407 212 8 42 367 53 451 6.67e-33 128
MS.gene00322.t1 AT1G62110 26.923 390 206 7 41 353 52 439 2.70e-32 127
MS.gene00322.t1 AT1G62150 25.750 400 219 9 35 359 52 448 4.78e-31 123
MS.gene00322.t1 AT5G23930 27.506 389 204 7 41 358 55 436 1.96e-29 119
MS.gene00322.t1 AT1G62010 26.611 357 187 7 54 359 57 389 1.28e-28 115
MS.gene00322.t1 AT1G56380 25.135 370 213 11 42 359 28 385 6.14e-23 99.8
MS.gene00322.t1 AT1G56380 25.135 370 213 11 42 359 17 374 6.22e-23 99.8
MS.gene00322.t1 AT1G79220 24.026 308 191 9 79 380 119 389 2.05e-13 71.6

Find 75 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCATACCTCATTCACAATTT+TGG 0.106320 2.1:-56266635 MS.gene00322:CDS
GTGAAGGAGTTAAAGGATTT+AGG 0.142526 2.1:-56266119 MS.gene00322:CDS
AATTGCAATCCAACTTTATT+TGG 0.152258 2.1:-56266263 MS.gene00322:CDS
TTGCAATGACAGCGCGTATT+TGG 0.169327 2.1:+56266498 None:intergenic
TGCTAAAACACCAAGGGTTT+TGG 0.198044 2.1:-56265868 MS.gene00322:CDS
TAAACTCAGTGATGAGCTTT+TGG 0.230631 2.1:-56265923 MS.gene00322:CDS
TTTCTTCATAAAGCTTTAAT+AGG 0.257224 2.1:+56265682 None:intergenic
GAAGAGAGGTTTGCATAAAA+AGG 0.278062 2.1:-56265790 MS.gene00322:CDS
TATGTTCCTTACTGCAAGTT+TGG 0.287367 2.1:-56266360 MS.gene00322:CDS
TGAAGGAGTTAAAGGATTTA+GGG 0.287415 2.1:-56266118 MS.gene00322:CDS
AGACCAGCCCCATTTCTTAA+AGG 0.311413 2.1:+56266010 None:intergenic
AAACTTTGGCAACACCCTTT+TGG 0.315392 2.1:+56266445 None:intergenic
AAATATTGCAAGATTGCTTC+GGG 0.322556 2.1:-56266183 MS.gene00322:CDS
GGTTTCGGATAAATATGTTT+TGG 0.343580 2.1:+56266055 None:intergenic
TGAGTTGGTGAAGGAGTTAA+AGG 0.346015 2.1:-56266126 MS.gene00322:CDS
AGTTGATACCTTTAAGAAAT+GGG 0.353795 2.1:-56266018 MS.gene00322:CDS
AAGTTGATACCTTTAAGAAA+TGG 0.365182 2.1:-56266019 MS.gene00322:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.1:-56265904 MS.gene00322:CDS
TGCAATGACAGCGCGTATTT+GGG 0.367219 2.1:+56266499 None:intergenic
AATAGTCCCAACCTATGAAT+TGG 0.380595 2.1:-56266330 MS.gene00322:CDS
CTACTTCTTCCAAATAAAGT+TGG 0.386610 2.1:+56266254 None:intergenic
CATGTATATAAATCGGTTTA+AGG 0.388072 2.1:-56265718 MS.gene00322:CDS
GTCAGAGTTAGAAGCACCTT+TGG 0.395726 2.1:+56266409 None:intergenic
TAAATATTGCAAGATTGCTT+CGG 0.406570 2.1:-56266184 MS.gene00322:CDS
GTATTTATAAACCAATTCAT+AGG 0.407861 2.1:+56266319 None:intergenic
AAGAAGTTGAGAACTGAATC+AGG 0.411972 2.1:+56266545 None:intergenic
TATAAACCAATTCATAGGTT+GGG 0.416412 2.1:+56266324 None:intergenic
GTCTCATCAGTGACTATAAA+CGG 0.428293 2.1:+56265750 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.1:-56265905 MS.gene00322:CDS
TCTATGACATGTATATAAAT+CGG 0.442482 2.1:-56265725 MS.gene00322:CDS
TTTCCTGCGATCCCTCCAAA+AGG 0.445420 2.1:-56266460 MS.gene00322:CDS
TCTTGTCCAAACTTGCAGTA+AGG 0.462468 2.1:+56266354 None:intergenic
GTGGAGAAATAGTGAGAAAA+GGG 0.466735 2.1:+56266689 None:intergenic
GGTGGAGAAATAGTGAGAAA+AGG 0.473547 2.1:+56266688 None:intergenic
TGGGTTTGGTGAGAAAGTTA+AGG 0.482786 2.1:+56266724 None:intergenic
TTTAACTCCTTCACCAACTC+AGG 0.483502 2.1:+56266128 None:intergenic
AAATAGTGAGAAAAGGGTAA+TGG 0.487797 2.1:+56266695 None:intergenic
TTATAAACCAATTCATAGGT+TGG 0.490431 2.1:+56266323 None:intergenic
TAAGGTCTTGAGATAGAGAA+GGG 0.500312 2.1:+56266742 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.1:-56265895 MS.gene00322:CDS
TATGATCCTTCGCTCCAGAC+TGG 0.512261 2.1:+56265842 None:intergenic
CTCAACTTCTTTAGAAACCA+TGG 0.514218 2.1:-56266533 MS.gene00322:CDS
AGAATCCAAAATTGTGAATG+AGG 0.517196 2.1:+56266630 None:intergenic
TTAAGGTCTTGAGATAGAGA+AGG 0.519722 2.1:+56266741 None:intergenic
AAACTTGCAGTAAGGAACAT+AGG 0.519839 2.1:+56266362 None:intergenic
CTTGAAATGAACATTGTAAG+TGG 0.523656 2.1:+56266580 None:intergenic
GAGGTATGACACTGCAAAAG+AGG 0.530330 2.1:+56266649 None:intergenic
TATTCCACAACTGAGGCTCT+TGG 0.536781 2.1:+56265819 None:intergenic
AACTTGCAGTAAGGAACATA+GGG 0.539419 2.1:+56266363 None:intergenic
TTCCTGCGATCCCTCCAAAA+GGG 0.542143 2.1:-56266459 MS.gene00322:CDS
ATACCTTTAAGAAATGGGGC+TGG 0.564816 2.1:-56266013 MS.gene00322:CDS
GGTTGAATCTGAAGTGTTGG+TGG 0.573845 2.1:+56266670 None:intergenic
GTTGATACCTTTAAGAAATG+GGG 0.574983 2.1:-56266017 MS.gene00322:CDS
GTGGAATATCTTCTGAAGAG+AGG 0.579571 2.1:-56265804 MS.gene00322:CDS
CATATTTATCCGAAACCCGA+TGG 0.584129 2.1:-56266049 MS.gene00322:CDS
ATCAAATTGTTGATTCAGAG+TGG 0.585441 2.1:-56266218 MS.gene00322:CDS
CAACTTGCCTGAGTTGGTGA+AGG 0.601746 2.1:-56266135 MS.gene00322:CDS
TTTGGGAATCAGAGAAACCA+TGG 0.602265 2.1:+56266516 None:intergenic
ATTGAATCTTGCACAAACCA+AGG 0.602352 2.1:-56265658 MS.gene00322:CDS
GGCCATTGCTAAAACACCAA+GGG 0.609965 2.1:-56265874 MS.gene00322:CDS
TGAGATAGAGAAGGGTTGAA+CGG 0.624735 2.1:+56266750 None:intergenic
GACAGGCAACTTGCCTGAGT+TGG 0.629550 2.1:-56266141 MS.gene00322:CDS
CATACCAAGAGCCTCAGTTG+TGG 0.635286 2.1:-56265823 MS.gene00322:CDS
ACATAGGGTTCTTAGTGACA+AGG 0.636619 2.1:+56266378 None:intergenic
AGAGGTTGAATCTGAAGTGT+TGG 0.638939 2.1:+56266667 None:intergenic
ACTGAGAAAATTGATTGTGA+AGG 0.638960 2.1:+56266089 None:intergenic
AATACGCGCTGTCATTGCAA+AGG 0.640391 2.1:-56266495 MS.gene00322:CDS
GGATCGCAGGAAAGCAAGCG+TGG 0.641477 2.1:+56266470 None:intergenic
TTTATTTGGAAGAAGTAGCG+TGG 0.642616 2.1:-56266249 MS.gene00322:CDS
GAAATGAACATTGTAAGTGG+AGG 0.651609 2.1:+56266583 None:intergenic
GTATATAAATCGGTTTAAGG+AGG 0.668406 2.1:-56265715 MS.gene00322:CDS
CAGAAGATATTCCACAACTG+AGG 0.677963 2.1:+56265812 None:intergenic
TGGCCATTGCTAAAACACCA+AGG 0.690744 2.1:-56265875 MS.gene00322:CDS
AATATTGCAAGATTGCTTCG+GGG 0.721927 2.1:-56266182 MS.gene00322:CDS
ATGGATGTTAACATACACTG+AGG 0.775832 2.1:+56265954 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTATTTATAAACCAATTCAT+AGG + chr2.1:56266089-56266108 None:intergenic 20.0%
!! TCTATGACATGTATATAAAT+CGG - chr2.1:56266680-56266699 MS.gene00322:CDS 20.0%
!!! AATATTAAGTTTTTCATGAC+AGG - chr2.1:56266247-56266266 MS.gene00322:CDS 20.0%
!!! TTTCTTCATAAAGCTTTAAT+AGG + chr2.1:56266726-56266745 None:intergenic 20.0%
! AATTGCAATCCAACTTTATT+TGG - chr2.1:56266142-56266161 MS.gene00322:CDS 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.1:56265977-56265996 None:intergenic 25.0%
! TAAATATTGCAAGATTGCTT+CGG - chr2.1:56266221-56266240 MS.gene00322:CDS 25.0%
! TATAAACCAATTCATAGGTT+GGG + chr2.1:56266084-56266103 None:intergenic 25.0%
! TTATAAACCAATTCATAGGT+TGG + chr2.1:56266085-56266104 None:intergenic 25.0%
!! AGTTGATACCTTTAAGAAAT+GGG - chr2.1:56266387-56266406 MS.gene00322:CDS 25.0%
!! CATGTATATAAATCGGTTTA+AGG - chr2.1:56266687-56266706 MS.gene00322:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.1:56265980-56265999 MS.gene00322:CDS 25.0%
!!! AAAGTTTTGAAGCAAATTGT+GGG + chr2.1:56265802-56265821 None:intergenic 25.0%
!!! AAGTTGATACCTTTAAGAAA+TGG - chr2.1:56266386-56266405 MS.gene00322:CDS 25.0%
!!! CACTGAGTTTATTTTATCAA+TGG + chr2.1:56266473-56266492 None:intergenic 25.0%
!!! TATCAACTTTTTCATTCCAT+CGG + chr2.1:56266375-56266394 None:intergenic 25.0%
AAATAGTGAGAAAAGGGTAA+TGG + chr2.1:56265713-56265732 None:intergenic 30.0%
AAATATTGCAAGATTGCTTC+GGG - chr2.1:56266222-56266241 MS.gene00322:CDS 30.0%
ACTGAGAAAATTGATTGTGA+AGG + chr2.1:56266319-56266338 None:intergenic 30.0%
AGAATCCAAAATTGTGAATG+AGG + chr2.1:56265778-56265797 None:intergenic 30.0%
CTACTTCTTCCAAATAAAGT+TGG + chr2.1:56266154-56266173 None:intergenic 30.0%
CTTGAAATGAACATTGTAAG+TGG + chr2.1:56265828-56265847 None:intergenic 30.0%
TGAAGGAGTTAAAGGATTTA+GGG - chr2.1:56266287-56266306 MS.gene00322:CDS 30.0%
! GGTTTCGGATAAATATGTTT+TGG + chr2.1:56266353-56266372 None:intergenic 30.0%
! GTATATAAATCGGTTTAAGG+AGG - chr2.1:56266690-56266709 MS.gene00322:CDS 30.0%
! GTTGATACCTTTAAGAAATG+GGG - chr2.1:56266388-56266407 MS.gene00322:CDS 30.0%
! TCATACCTCATTCACAATTT+TGG - chr2.1:56265770-56265789 MS.gene00322:CDS 30.0%
!! ATCAAATTGTTGATTCAGAG+TGG - chr2.1:56266187-56266206 MS.gene00322:CDS 30.0%
!!! AAAAGGGTAATGGAGTTTTT+GGG + chr2.1:56265703-56265722 None:intergenic 30.0%
!!! AAGTTTTGAAGCAAATTGTG+GGG + chr2.1:56265801-56265820 None:intergenic 30.0%
!!! ATCAACTTTTTCATTCCATC+GGG + chr2.1:56266374-56266393 None:intergenic 30.0%
!!! GAAAGTTTTGAAGCAAATTG+TGG + chr2.1:56265803-56265822 None:intergenic 30.0%
AAACTTGCAGTAAGGAACAT+AGG + chr2.1:56266046-56266065 None:intergenic 35.0%
AACTTGCAGTAAGGAACATA+GGG + chr2.1:56266045-56266064 None:intergenic 35.0%
AAGAAGTTGAGAACTGAATC+AGG + chr2.1:56265863-56265882 None:intergenic 35.0%
AATAGTCCCAACCTATGAAT+TGG - chr2.1:56266075-56266094 MS.gene00322:CDS 35.0%
AATATTGCAAGATTGCTTCG+GGG - chr2.1:56266223-56266242 MS.gene00322:CDS 35.0%
ATGGATGTTAACATACACTG+AGG + chr2.1:56266454-56266473 None:intergenic 35.0%
ATTGAATCTTGCACAAACCA+AGG - chr2.1:56266747-56266766 MS.gene00322:CDS 35.0%
GAAATGAACATTGTAAGTGG+AGG + chr2.1:56265825-56265844 None:intergenic 35.0%
GAAGAGAGGTTTGCATAAAA+AGG - chr2.1:56266615-56266634 MS.gene00322:CDS 35.0%
GTCTCATCAGTGACTATAAA+CGG + chr2.1:56266658-56266677 None:intergenic 35.0%
GTGAAGGAGTTAAAGGATTT+AGG - chr2.1:56266286-56266305 MS.gene00322:CDS 35.0%
GTGGAGAAATAGTGAGAAAA+GGG + chr2.1:56265719-56265738 None:intergenic 35.0%
TAAGGTCTTGAGATAGAGAA+GGG + chr2.1:56265666-56265685 None:intergenic 35.0%
TATGTTCCTTACTGCAAGTT+TGG - chr2.1:56266045-56266064 MS.gene00322:CDS 35.0%
TTAAGGTCTTGAGATAGAGA+AGG + chr2.1:56265667-56265686 None:intergenic 35.0%
TTTATTTGGAAGAAGTAGCG+TGG - chr2.1:56266156-56266175 MS.gene00322:CDS 35.0%
! AAACTCAGTGATGAGCTTTT+GGG - chr2.1:56266483-56266502 MS.gene00322:CDS 35.0%
! CTCAACTTCTTTAGAAACCA+TGG - chr2.1:56265872-56265891 MS.gene00322:CDS 35.0%
! TAAACTCAGTGATGAGCTTT+TGG - chr2.1:56266482-56266501 MS.gene00322:CDS 35.0%
! TGAGAACTGAATCAGGTTTT+CGG + chr2.1:56265856-56265875 None:intergenic 35.0%
! TTTTTCATTCCATCGGGTTT+CGG + chr2.1:56266368-56266387 None:intergenic 35.0%
!!! GAAAAGGGTAATGGAGTTTT+TGG + chr2.1:56265704-56265723 None:intergenic 35.0%
!!! TATCATGCCAGTTTTTTCCT+TGG + chr2.1:56266767-56266786 None:intergenic 35.0%
ACATAGGGTTCTTAGTGACA+AGG + chr2.1:56266030-56266049 None:intergenic 40.0%
CAGAAGATATTCCACAACTG+AGG + chr2.1:56266596-56266615 None:intergenic 40.0%
CATATTTATCCGAAACCCGA+TGG - chr2.1:56266356-56266375 MS.gene00322:CDS 40.0%
GCACAAACCAAGGAAAAAAC+TGG - chr2.1:56266757-56266776 MS.gene00322:CDS 40.0%
GGTGGAGAAATAGTGAGAAA+AGG + chr2.1:56265720-56265739 None:intergenic 40.0%
TCTTGTCCAAACTTGCAGTA+AGG + chr2.1:56266054-56266073 None:intergenic 40.0%
TGAGATAGAGAAGGGTTGAA+CGG + chr2.1:56265658-56265677 None:intergenic 40.0%
TTTAACTCCTTCACCAACTC+AGG + chr2.1:56266280-56266299 None:intergenic 40.0%
TTTGGGAATCAGAGAAACCA+TGG + chr2.1:56265892-56265911 None:intergenic 40.0%
! AAACTTTGGCAACACCCTTT+TGG + chr2.1:56265963-56265982 None:intergenic 40.0%
! AGAACTGAATCAGGTTTTCG+GGG + chr2.1:56265854-56265873 None:intergenic 40.0%
! AGAGGTTGAATCTGAAGTGT+TGG + chr2.1:56265741-56265760 None:intergenic 40.0%
! ATACCTTTAAGAAATGGGGC+TGG - chr2.1:56266392-56266411 MS.gene00322:CDS 40.0%
! CTAAAACACCAAGGGTTTTG+GGG - chr2.1:56266539-56266558 MS.gene00322:CDS 40.0%
! GAGAACTGAATCAGGTTTTC+GGG + chr2.1:56265855-56265874 None:intergenic 40.0%
! GCTAAAACACCAAGGGTTTT+GGG - chr2.1:56266538-56266557 MS.gene00322:CDS 40.0%
! GTGGAATATCTTCTGAAGAG+AGG - chr2.1:56266601-56266620 MS.gene00322:CDS 40.0%
! TGCTAAAACACCAAGGGTTT+TGG - chr2.1:56266537-56266556 MS.gene00322:CDS 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.1:56266496-56266515 MS.gene00322:CDS 40.0%
!! TAAAACACCAAGGGTTTTGG+GGG - chr2.1:56266540-56266559 MS.gene00322:CDS 40.0%
!! TGAGTTGGTGAAGGAGTTAA+AGG - chr2.1:56266279-56266298 MS.gene00322:CDS 40.0%
!! TGGGTTTGGTGAGAAAGTTA+AGG + chr2.1:56265684-56265703 None:intergenic 40.0%
!!! AACCCTTGGTGTTTTAGCAA+TGG + chr2.1:56266536-56266555 None:intergenic 40.0%
!!! GGTAATGGAGTTTTTGGGTT+TGG + chr2.1:56265698-56265717 None:intergenic 40.0%
AATACGCGCTGTCATTGCAA+AGG - chr2.1:56265910-56265929 MS.gene00322:CDS 45.0%
AGACCAGCCCCATTTCTTAA+AGG + chr2.1:56266398-56266417 None:intergenic 45.0%
GAGGTATGACACTGCAAAAG+AGG + chr2.1:56265759-56265778 None:intergenic 45.0%
GGCCATTGCTAAAACACCAA+GGG - chr2.1:56266531-56266550 MS.gene00322:CDS 45.0%
TATTCCACAACTGAGGCTCT+TGG + chr2.1:56266589-56266608 None:intergenic 45.0%
TGCAATGACAGCGCGTATTT+GGG + chr2.1:56265909-56265928 None:intergenic 45.0%
TGGCCATTGCTAAAACACCA+AGG - chr2.1:56266530-56266549 MS.gene00322:CDS 45.0%
TTGCAATGACAGCGCGTATT+TGG + chr2.1:56265910-56265929 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.1:56266501-56266520 MS.gene00322:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.1:56266500-56266519 MS.gene00322:CDS 45.0%
! TTTGGCAACACCCTTTTGGA+GGG + chr2.1:56265959-56265978 None:intergenic 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.1:56266495-56266514 MS.gene00322:CDS 45.0%
!! GGTTGAATCTGAAGTGTTGG+TGG + chr2.1:56265738-56265757 None:intergenic 45.0%
!! GTCAGAGTTAGAAGCACCTT+TGG + chr2.1:56265999-56266018 None:intergenic 45.0%
CATACCAAGAGCCTCAGTTG+TGG - chr2.1:56266582-56266601 MS.gene00322:CDS 50.0%
TATGATCCTTCGCTCCAGAC+TGG + chr2.1:56266566-56266585 None:intergenic 50.0%
TTCCTGCGATCCCTCCAAAA+GGG - chr2.1:56265946-56265965 MS.gene00322:CDS 50.0%
TTTCCTGCGATCCCTCCAAA+AGG - chr2.1:56265945-56265964 MS.gene00322:CDS 50.0%
! CTTTGGCAACACCCTTTTGG+AGG + chr2.1:56265960-56265979 None:intergenic 50.0%
!! CAACTTGCCTGAGTTGGTGA+AGG - chr2.1:56266270-56266289 MS.gene00322:CDS 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.1:56266510-56266529 MS.gene00322:CDS 50.0%
GACAGGCAACTTGCCTGAGT+TGG - chr2.1:56266264-56266283 MS.gene00322:CDS 55.0%
GGATCGCAGGAAAGCAAGCG+TGG + chr2.1:56265938-56265957 None:intergenic 60.0%
! AGACTGGCCCCCAAAACCCT+TGG + chr2.1:56266550-56266569 None:intergenic 60.0%
!! CACCCTTTTGGAGGGATCGC+AGG + chr2.1:56265951-56265970 None:intergenic 60.0%
!!! AAGGGTTTTGGGGGCCAGTC+TGG - chr2.1:56266549-56266568 MS.gene00322:CDS 60.0%
! TGGGGGCCAGTCTGGAGCGA+AGG - chr2.1:56266557-56266576 MS.gene00322:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56265638 56266789 56265638 ID=MS.gene00322
chr2.1 mRNA 56265638 56266789 56265638 ID=MS.gene00322.t1;Parent=MS.gene00322
chr2.1 exon 56265638 56266789 56265638 ID=MS.gene00322.t1.exon1;Parent=MS.gene00322.t1
chr2.1 CDS 56265638 56266789 56265638 ID=cds.MS.gene00322.t1;Parent=MS.gene00322.t1
Gene Sequence

>MS.gene00322

ATGTTTAACACTCATCTCCGTTCAACCCTTCTCTATCTCAAGACCTTAACTTTCTCACCAAACCCAAAAACTCCATTACCCTTTTCTCACTATTTCTCCACCAACACTTCAGATTCAACCTCTTTTGCAGTGTCATACCTCATTCACAATTTTGGATTCTCCCCACAATTTGCTTCAAAACTTTCCTCCACTTACAATGTTCATTTCAAGACTCCCCGAAAACCTGATTCAGTTCTCAACTTCTTTAGAAACCATGGTTTCTCTGATTCCCAAATACGCGCTGTCATTGCAAAGGAACCACGCTTGCTTTCCTGCGATCCCTCCAAAAGGGTGTTGCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTAACTCTGACATTGTTAACCTTGTCACTAAGAACCCTATGTTCCTTACTGCAAGTTTGGACAAGAAAATAGTCCCAACCTATGAATTGGTTTATAAATACTTGCAATCTGATAAACACATTCTTGCTTTGTTGAATTGCAATCCAACTTTATTTGGAAGAAGTAGCGTGGAACATAACATCAAATTGTTGATTCAGAGTGGAGTGACAGACATAAATATTGCAAGATTGCTTCGGGGCAATATTAAGTTTTTCATGACAGGCAACTTGCCTGAGTTGGTGAAGGAGTTAAAGGATTTAGGGTTTCATCCTTCACAATCAATTTTCTCAGTAGCATTGATTGCCAAAACATATTTATCCGAAACCCGATGGAATGAAAAAGTTGATACCTTTAAGAAATGGGGCTGGTCTGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCAGTGTATGTTAACATCCATTGATAAAATAAACTCAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCTAAAACACCAAGGGTTTTGGGGGCCAGTCTGGAGCGAAGGATCATACCAAGAGCCTCAGTTGTGGAATATCTTCTGAAGAGAGGTTTGCATAAAAAGGAAGCAAGTTTAACTTCTCCGTTTATAGTCACTGATGAGACATTCTATGACATGTATATAAATCGGTTTAAGGAGGAGTCTTCTTACCTATTAAAGCTTTATGAAGAAAAATTGAATCTTGCACAAACCAAGGAAAAAACTGGCATGATATGA
Protein sequence

>MS.gene00322.t1

MFNTHLRSTLLYLKTLTFSPNPKTPLPFSHYFSTNTSDSTSFAVSYLIHNFGFSPQFASKLSSTYNVHFKTPRKPDSVLNFFRNHGFSDSQIRAVIAKEPRLLSCDPSKRVLPKFQFLLSKGASNSDIVNLVTKNPMFLTASLDKKIVPTYELVYKYLQSDKHILALLNCNPTLFGRSSVEHNIKLLIQSGVTDINIARLLRGNIKFFMTGNLPELVKELKDLGFHPSQSIFSVALIAKTYLSETRWNEKVDTFKKWGWSDEDVLEAFKKQPQCMLTSIDKINSVMSFWVNQLGWDAMAIAKTPRVLGASLERRIIPRASVVEYLLKRGLHKKEASLTSPFIVTDETFYDMYINRFKEESSYLLKLYEEKLNLAQTKEKTGMI