AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68901

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68901.t1 MTR_2g437020 91.202 341 30 0 14 354 43 383 0.0 629
MS.gene68901.t1 MTR_2g437060 67.787 357 102 3 6 351 41 395 3.99e-169 477
MS.gene68901.t1 MTR_2g437080 67.742 341 107 2 13 352 43 381 7.04e-167 471
MS.gene68901.t1 MTR_2g437030 67.251 342 109 2 14 354 130 469 1.24e-160 458
MS.gene68901.t1 MTR_2g436400 67.164 335 107 2 12 345 49 381 1.83e-160 454
MS.gene68901.t1 MTR_2g436440 66.082 342 113 2 14 354 50 389 1.54e-157 447
MS.gene68901.t1 MTR_2g437040 64.223 341 119 2 12 351 42 380 1.61e-153 437
MS.gene68901.t1 MTR_2g437120 56.305 341 142 5 13 349 54 391 5.51e-124 363
MS.gene68901.t1 MTR_4g119550 54.706 340 148 4 13 350 54 389 6.34e-124 362
MS.gene68901.t1 MTR_2g437960 53.216 342 154 3 14 351 57 396 4.36e-123 360
MS.gene68901.t1 MTR_2g437960 52.907 344 156 3 13 352 56 397 9.31e-123 360
MS.gene68901.t1 MTR_2g438010 54.464 336 147 3 14 345 74 407 5.58e-121 355
MS.gene68901.t1 MTR_2g437170 54.386 342 148 4 14 349 59 398 6.00e-120 352
MS.gene68901.t1 MTR_2g437160 53.824 340 152 3 13 349 66 403 1.42e-119 352
MS.gene68901.t1 MTR_2g437150 53.801 342 150 4 14 349 59 398 1.69e-119 351
MS.gene68901.t1 MTR_2g437990 53.982 339 151 4 14 349 54 390 3.87e-119 351
MS.gene68901.t1 MTR_2g437200 50.678 369 139 5 12 341 34 398 4.15e-118 347
MS.gene68901.t1 MTR_2g076320 52.941 340 154 3 14 349 54 391 4.50e-117 345
MS.gene68901.t1 MTR_2g437180 51.429 350 160 5 3 349 46 388 6.36e-115 339
MS.gene68901.t1 MTR_2g437240 49.734 376 146 5 13 349 35 406 3.78e-114 338
MS.gene68901.t1 MTR_2g437260 49.851 335 165 2 12 345 38 370 4.23e-114 337
MS.gene68901.t1 MTR_2g437100 54.762 336 144 4 12 341 62 395 5.21e-114 337
MS.gene68901.t1 MTR_2g437130 51.404 356 158 4 5 349 28 379 6.69e-114 336
MS.gene68901.t1 MTR_2g438020 48.158 380 150 6 7 347 72 443 3.08e-108 325
MS.gene68901.t1 MTR_2g437940 49.573 351 139 5 3 349 46 362 5.60e-105 313
MS.gene68901.t1 MTR_2g436460 48.810 336 166 4 11 344 48 379 1.29e-104 312
MS.gene68901.t1 MTR_4g119580 46.037 328 172 3 13 339 47 370 7.51e-92 280
MS.gene68901.t1 MTR_2g019840 47.076 342 172 6 12 350 45 380 1.84e-90 276
MS.gene68901.t1 MTR_4g120380 46.483 327 170 3 14 339 48 370 1.12e-87 269
MS.gene68901.t1 MTR_2g437090 72.941 170 45 1 185 353 1 170 1.26e-85 256
MS.gene68901.t1 MTR_4g120380 47.557 307 157 2 14 320 48 350 9.15e-85 263
MS.gene68901.t1 MTR_2g019810 45.946 333 169 7 13 341 34 359 3.24e-84 259
MS.gene68901.t1 MTR_2g437160 55.941 202 83 3 152 349 113 312 1.48e-67 215
MS.gene68901.t1 MTR_2g437160 43.820 89 46 1 12 100 65 149 3.76e-16 78.6
MS.gene68901.t1 MTR_2g436370 59.184 147 60 0 12 158 48 194 4.12e-53 174
MS.gene68901.t1 MTR_3g450500 32.827 329 211 3 13 335 41 365 9.39e-53 179
MS.gene68901.t1 MTR_4g119570 37.450 251 121 3 90 339 14 229 3.12e-43 149
MS.gene68901.t1 MTR_4g007490 31.193 327 218 4 13 335 40 363 3.03e-42 151
MS.gene68901.t1 MTR_8g080990 29.412 323 221 2 17 335 48 367 6.01e-33 126
MS.gene68901.t1 MTR_8g081000 27.019 322 227 2 26 346 70 384 1.65e-32 125
MS.gene68901.t1 MTR_8g081000 27.019 322 227 2 26 346 70 384 1.86e-32 125
MS.gene68901.t1 MTR_8g081000 27.019 322 227 2 26 346 70 384 2.85e-32 125
MS.gene68901.t1 MTR_4g049400 47.475 99 52 0 12 110 52 150 3.13e-23 97.1
MS.gene68901.t1 MTR_2g436380 70.492 61 18 0 185 245 1 61 4.67e-22 94.7
MS.gene68901.t1 MTR_3g085240 44.944 89 32 1 244 332 176 247 4.56e-16 77.4
MS.gene68901.t1 MTR_3g085240 34.959 123 69 3 12 133 59 171 8.98e-11 62.0
MS.gene68901.t1 MTR_8g012210 22.180 266 192 5 55 315 62 317 1.10e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68901.t1 AT5G07900 34.940 332 207 5 13 338 71 399 1.10e-63 208
MS.gene68901.t1 AT1G21150 31.908 304 200 3 7 306 52 352 1.76e-43 155
MS.gene68901.t1 AT1G21150 31.908 304 200 3 7 306 116 416 8.19e-43 154
MS.gene68901.t1 AT1G21150 31.773 299 197 3 12 306 21 316 2.10e-42 151
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61970 27.606 355 206 9 13 325 54 399 1.11e-29 118
MS.gene68901.t1 AT1G61980 28.371 356 204 9 13 326 54 400 3.43e-29 117
MS.gene68901.t1 AT1G61980 28.371 356 204 9 13 326 54 400 3.43e-29 117
MS.gene68901.t1 AT1G61980 28.371 356 204 9 13 326 54 400 3.43e-29 117
MS.gene68901.t1 AT5G64950 27.304 293 198 4 14 297 42 328 3.61e-29 116
MS.gene68901.t1 AT1G61990 28.529 340 182 8 13 309 54 375 5.49e-29 116
MS.gene68901.t1 AT1G61990 28.529 340 182 8 13 309 54 375 5.49e-29 116
MS.gene68901.t1 AT1G62120 27.410 332 191 8 13 302 58 381 1.08e-25 107
MS.gene68901.t1 AT3G46950 30.924 249 157 6 13 257 54 291 2.32e-25 106
MS.gene68901.t1 AT1G62010 26.935 323 186 6 24 302 43 359 2.29e-23 100
MS.gene68901.t1 AT5G23930 25.974 385 203 11 13 323 57 433 1.26e-21 95.9
MS.gene68901.t1 AT1G62085 25.000 364 192 10 13 301 57 414 1.39e-20 92.8
MS.gene68901.t1 AT1G62085 25.000 364 192 10 13 301 57 414 1.39e-20 92.8
MS.gene68901.t1 AT1G61960 28.745 247 166 4 13 255 54 294 5.50e-20 91.3
MS.gene68901.t1 AT1G62110 27.016 248 170 4 13 255 54 295 2.36e-18 86.3
MS.gene68901.t1 AT1G56380 24.789 355 219 9 12 323 17 366 3.76e-17 82.4
MS.gene68901.t1 AT1G56380 24.789 355 219 9 12 323 28 377 4.02e-17 82.4
MS.gene68901.t1 AT1G62150 24.590 244 179 3 13 254 60 300 1.36e-16 81.3

Find 73 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.159722 2.4:+55023488 MS.gene68901:CDS
TCAATTTAGTGATGAACTTT+TGG 0.199642 2.4:+55024206 MS.gene68901:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2.4:+55023663 MS.gene68901:CDS
GAAATTGGTGGAGGAATTAA+AGG 0.238950 2.4:+55024003 MS.gene68901:CDS
GGTGGAGGAATTAAAGGATT+TGG 0.242361 2.4:+55024009 MS.gene68901:CDS
ATCAACTTCTTTAGAAACTA+TGG 0.267482 2.4:+55023590 MS.gene68901:CDS
GTGGAGGAATTAAAGGATTT+GGG 0.314616 2.4:+55024010 MS.gene68901:CDS
AGACCAACCCCACTTCTTAA+AGG 0.324708 2.4:-55024119 None:intergenic
GTTTCTTGATAAGTGTATAA+AGG 0.325915 2.4:+55024402 MS.gene68901:CDS
TTCGAAAAGAAATGGAAATA+TGG 0.345689 2.4:-55023214 None:intergenic
TTAAAAGAATGCTTTGGTTA+AGG 0.353006 2.4:+55023945 MS.gene68901:CDS
GACACATGACATGCTGAAAT+TGG 0.353711 2.4:+55023988 MS.gene68901:CDS
CCTGTTAGTTCGAAAAGAAA+TGG 0.356466 2.4:-55023222 None:intergenic
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2.4:-55023639 None:intergenic
TTTCTTGATAAGTGTATAAA+GGG 0.396797 2.4:+55024403 MS.gene68901:CDS
ATGTTCTACAAGCATTTAGA+AGG 0.400394 2.4:+55024149 MS.gene68901:CDS
GTCAGAGGTAGAAGCGCCTT+TGG 0.405353 2.4:-55023714 None:intergenic
TGCGTATGTGGAGCAAAGTC+TGG 0.409089 2.4:-55023531 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2.4:+55024325 MS.gene68901:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2.4:-55023624 None:intergenic
TAAGTGTATAAAGGGTATTA+AGG 0.419835 2.4:+55024411 MS.gene68901:CDS
GAATCTATAAATCAATTGAT+AGG 0.434987 2.4:-55023804 None:intergenic
ATTCGCAATCCATCTTTCTT+CGG 0.435209 2.4:+55023860 MS.gene68901:CDS
CAAACATTAAAAGAATGCTT+TGG 0.436839 2.4:+55023939 MS.gene68901:CDS
CTTAAAACTAATTGCGTATG+TGG 0.438575 2.4:-55023543 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2.4:+55024441 MS.gene68901:CDS
TTGGGTCAATCAGTTAGGTT+GGG 0.447898 2.4:+55024225 MS.gene68901:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2.4:+55024281 MS.gene68901:CDS
TATAAATCAATTGATAGGCT+TGG 0.456794 2.4:-55023799 None:intergenic
AAGAAGTTGATAACAGAGTC+AGG 0.461266 2.4:-55023578 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2.4:-55023625 None:intergenic
TTTGGGTCAATCAGTTAGGT+TGG 0.464253 2.4:+55024224 MS.gene68901:CDS
AATGCTTTGGTTAAGGAGTC+GGG 0.471203 2.4:+55023952 MS.gene68901:CDS
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2.4:+55023664 MS.gene68901:CDS
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2.4:-55023921 None:intergenic
GAATGCTTTGGTTAAGGAGT+CGG 0.487572 2.4:+55023951 MS.gene68901:CDS
ATCTGTAAGGAAAACATTGT+GGG 0.488825 2.4:+55024081 MS.gene68901:CDS
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2.4:+55024282 MS.gene68901:CDS
AAAGTCTGGAAGCAGATTGT+GGG 0.509506 2.4:-55023517 None:intergenic
CGAGCGTCTCCGAAGAAAGA+TGG 0.514763 2.4:-55023869 None:intergenic
TGCATGCCAAACTATCTGTA+AGG 0.519763 2.4:+55024068 MS.gene68901:CDS
CAAAGTCTGGAAGCAGATTG+TGG 0.519859 2.4:-55023518 None:intergenic
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2.4:+55024293 MS.gene68901:CDS
ACTACGTCTTACCATTTCAA+AGG 0.541064 2.4:+55023628 MS.gene68901:CDS
ATTAGATTGCTGATTGCCAA+TGG 0.546889 2.4:+55023905 MS.gene68901:CDS
AAACTGATGGTCCTTTGGCA+AGG 0.547751 2.4:-55024258 None:intergenic
AGTCGATGCCTTTAAGAAGT+GGG 0.559365 2.4:+55024111 MS.gene68901:CDS
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2.4:+55024247 MS.gene68901:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2.4:-55024310 None:intergenic
AACTGATGGTCCTTTGGCAA+GGG 0.571808 2.4:-55024257 None:intergenic
TAGATTCTTGCAATCAGACA+AGG 0.576536 2.4:+55023820 MS.gene68901:CDS
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2.4:+55024294 MS.gene68901:CDS
AAGTCGATGCCTTTAAGAAG+TGG 0.577016 2.4:+55024110 MS.gene68901:CDS
TATCTGTAAGGAAAACATTG+TGG 0.577111 2.4:+55024080 MS.gene68901:CDS
GCCAAAATTGTTGATGAGGT+AGG 0.577466 2.4:-55023489 None:intergenic
AAGGCAACCACATTGTATGT+TGG 0.594814 2.4:+55024168 MS.gene68901:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2.4:-55024306 None:intergenic
AAAAGCCAAAATTGTTGATG+AGG 0.598384 2.4:-55023493 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.598873 2.4:+55024116 MS.gene68901:CDS
GTCGATGCCTTTAAGAAGTG+GGG 0.602513 2.4:+55024112 MS.gene68901:CDS
GGCATGCAATGCTACACCAA+AGG 0.603475 2.4:-55024053 None:intergenic
GAAAATGTTAACAATGTCAG+AGG 0.609432 2.4:-55023729 None:intergenic
ACATGACATGCTGAAATTGG+TGG 0.610610 2.4:+55023991 MS.gene68901:CDS
TTTCTTCGGAGACGCTCGTG+TGG 0.619956 2.4:+55023874 MS.gene68901:CDS
TGACATGCTGAAATTGGTGG+AGG 0.631062 2.4:+55023994 MS.gene68901:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2.4:+55024468 MS.gene68901:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2.4:-55023986 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2.4:-55023653 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2.4:+55023636 MS.gene68901:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2.4:-55024317 None:intergenic
AAGTCTGGAAGCAGATTGTG+GGG 0.672466 2.4:-55023516 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.672936 2.4:+55023910 MS.gene68901:CDS
ATGGATACCAACATACAATG+TGG 0.711327 2.4:-55024175 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAAAGAAAAAACTGA+TGG - chr2.4:55024274-55024293 None:intergenic 20.0%
!! GAATCTATAAATCAATTGAT+AGG - chr2.4:55023807-55023826 None:intergenic 20.0%
!! TTATCTATTAAAGTTGTATG+AGG + chr2.4:55024441-55024460 MS.gene68901:CDS 20.0%
!! TTTCTTGATAAGTGTATAAA+GGG + chr2.4:55024403-55024422 MS.gene68901:CDS 20.0%
!!! AAGATATAAACGATTTTCAT+CGG + chr2.4:55023061-55023080 MS.gene68901:intron 20.0%
!!! CACTAAATTGATTTTATCAA+TGG - chr2.4:55024197-55024216 None:intergenic 20.0%
!!! CTTGGATAATTTTATTATTG+AGG - chr2.4:55023784-55023803 None:intergenic 20.0%
! CAAACATTAAAAGAATGCTT+TGG + chr2.4:55023939-55023958 MS.gene68901:CDS 25.0%
! CAATCAAATTCCAAGAATTA+GGG + chr2.4:55023263-55023282 MS.gene68901:intron 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.4:55023695-55023714 None:intergenic 25.0%
! GTTTCTTGATAAGTGTATAA+AGG + chr2.4:55024402-55024421 MS.gene68901:CDS 25.0%
! TATAAATCAATTGATAGGCT+TGG - chr2.4:55023802-55023821 None:intergenic 25.0%
! TTAAAAGAATGCTTTGGTTA+AGG + chr2.4:55023945-55023964 MS.gene68901:CDS 25.0%
! TTCGAAAAGAAATGGAAATA+TGG - chr2.4:55023217-55023236 None:intergenic 25.0%
!! ATCAACTTCTTTAGAAACTA+TGG + chr2.4:55023590-55023609 MS.gene68901:CDS 25.0%
!! CAATTTAGTGATGAACTTTT+GGG + chr2.4:55024207-55024226 MS.gene68901:CDS 25.0%
!! CATTCTTTTAATGTTTGAGT+CGG - chr2.4:55023936-55023955 None:intergenic 25.0%
!! TCAATTTAGTGATGAACTTT+TGG + chr2.4:55024206-55024225 MS.gene68901:CDS 25.0%
!! TTCTTTAAGTTTCGAAAAGA+GGG + chr2.4:55024282-55024301 MS.gene68901:CDS 25.0%
!! TTTCTTTAAGTTTCGAAAAG+AGG + chr2.4:55024281-55024300 MS.gene68901:CDS 25.0%
!!! TAAGTGTATAAAGGGTATTA+AGG + chr2.4:55024411-55024430 MS.gene68901:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.4:55023698-55023717 MS.gene68901:CDS 25.0%
AAAAGCCAAAATTGTTGATG+AGG - chr2.4:55023496-55023515 None:intergenic 30.0%
ATCTGTAAGGAAAACATTGT+GGG + chr2.4:55024081-55024100 MS.gene68901:CDS 30.0%
ATGTTCTACAAGCATTTAGA+AGG + chr2.4:55024149-55024168 MS.gene68901:CDS 30.0%
CTTAAAACTAATTGCGTATG+TGG - chr2.4:55023546-55023565 None:intergenic 30.0%
GAAAAATGGAGAGAAAATGT+TGG - chr2.4:55023434-55023453 None:intergenic 30.0%
GAAAATGTTAACAATGTCAG+AGG - chr2.4:55023732-55023751 None:intergenic 30.0%
GCAATCAAATTCCAAGAATT+AGG + chr2.4:55023262-55023281 MS.gene68901:intron 30.0%
GGTAATAACGTTGAAAAAGA+GGG - chr2.4:55023403-55023422 None:intergenic 30.0%
TATCTGTAAGGAAAACATTG+TGG + chr2.4:55024080-55024099 MS.gene68901:CDS 30.0%
TGAATTTGAACCCTAATTCT+TGG - chr2.4:55023276-55023295 None:intergenic 30.0%
! TAGAGCTTTTCTCTATTTCA+AGG + chr2.4:55023346-55023365 MS.gene68901:intron 30.0%
! TGTTTTCCTTACAGATAGTT+TGG - chr2.4:55024077-55024096 None:intergenic 30.0%
! TTATCAAGAAACAGCTTTTC+AGG - chr2.4:55024394-55024413 None:intergenic 30.0%
! TTGCAATATCTTCTGAAGAA+AGG + chr2.4:55024325-55024344 MS.gene68901:CDS 30.0%
!! TATTATTGAGGCTTGTTTTC+AGG - chr2.4:55023772-55023791 None:intergenic 30.0%
AACGTTGAAAAAGAGGGTTA+GGG - chr2.4:55023397-55023416 None:intergenic 35.0%
AATTTCAGCATGTCATGTGT+CGG - chr2.4:55023989-55024008 None:intergenic 35.0%
ACTACGTCTTACCATTTCAA+AGG + chr2.4:55023628-55023647 MS.gene68901:CDS 35.0%
AGAAAAAACTGATGGTCCTT+TGG - chr2.4:55024266-55024285 None:intergenic 35.0%
ATATGGAAGCTGAGAACAAA+TGG - chr2.4:55023200-55023219 None:intergenic 35.0%
ATGGATACCAACATACAATG+TGG - chr2.4:55024178-55024197 None:intergenic 35.0%
ATTCGCAATCCATCTTTCTT+CGG + chr2.4:55023860-55023879 MS.gene68901:CDS 35.0%
CAGAAGATATTGCAATACAG+AGG - chr2.4:55024320-55024339 None:intergenic 35.0%
CCTGTTAGTTCGAAAAGAAA+TGG - chr2.4:55023225-55023244 None:intergenic 35.0%
GAAATTGGTGGAGGAATTAA+AGG + chr2.4:55024003-55024022 MS.gene68901:CDS 35.0%
GAAGAAAGGTTTGCGAAAAA+AGG + chr2.4:55024339-55024358 MS.gene68901:CDS 35.0%
GCTACTTCAAAAACAACCTT+TGG + chr2.4:55024037-55024056 MS.gene68901:CDS 35.0%
GGGTAATAACGTTGAAAAAG+AGG - chr2.4:55023404-55023423 None:intergenic 35.0%
GTGGAGGAATTAAAGGATTT+GGG + chr2.4:55024010-55024029 MS.gene68901:CDS 35.0%
TAACGTTGAAAAAGAGGGTT+AGG - chr2.4:55023398-55023417 None:intergenic 35.0%
TAGATTCTTGCAATCAGACA+AGG + chr2.4:55023820-55023839 MS.gene68901:CDS 35.0%
TATGGAAGCTGAGAACAAAT+GGG - chr2.4:55023199-55023218 None:intergenic 35.0%
TGAAATGGTAAGACGTAGTT+GGG - chr2.4:55023627-55023646 None:intergenic 35.0%
TTACCATTTCAAAGGAACCA+TGG + chr2.4:55023636-55023655 MS.gene68901:CDS 35.0%
TTGAAATGGTAAGACGTAGT+TGG - chr2.4:55023628-55023647 None:intergenic 35.0%
! ATTAGATTGCTGATTGCCAA+TGG + chr2.4:55023905-55023924 MS.gene68901:CDS 35.0%
! CCATTTCTTTTCGAACTAAC+AGG + chr2.4:55023222-55023241 MS.gene68901:CDS 35.0%
! GATAACAGAGTCAGGTTTTT+GGG - chr2.4:55023573-55023592 None:intergenic 35.0%
! TCCTACCTCATCAACAATTT+TGG + chr2.4:55023488-55023507 MS.gene68901:CDS 35.0%
! TGATAACAGAGTCAGGTTTT+TGG - chr2.4:55023574-55023593 None:intergenic 35.0%
! TTTGGCAAAACCCTTTTAGA+AGG - chr2.4:55023677-55023696 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+AGG + chr2.4:55024220-55024239 MS.gene68901:CDS 35.0%
!! AAGAAGTTGATAACAGAGTC+AGG - chr2.4:55023581-55023600 None:intergenic 35.0%
!! AGAGAAAATGTTGGTGATGA+TGG - chr2.4:55023425-55023444 None:intergenic 35.0%
!! GAGAAAATGTTGGTGATGAT+GGG - chr2.4:55023424-55023443 None:intergenic 35.0%
!! TTGGCAAAACCCTTTTAGAA+GGG - chr2.4:55023676-55023695 None:intergenic 35.0%
AAAGTCTGGAAGCAGATTGT+GGG - chr2.4:55023520-55023539 None:intergenic 40.0%
AAGGCAACCACATTGTATGT+TGG + chr2.4:55024168-55024187 MS.gene68901:CDS 40.0%
AATGCTTTGGTTAAGGAGTC+GGG + chr2.4:55023952-55023971 MS.gene68901:CDS 40.0%
ACATGACATGCTGAAATTGG+TGG + chr2.4:55023991-55024010 MS.gene68901:CDS 40.0%
ACTCAATCTTGCTTACACCA+TGG + chr2.4:55024468-55024487 MS.gene68901:CDS 40.0%
AGAGAAAAGCTCTATGACGA+TGG - chr2.4:55023340-55023359 None:intergenic 40.0%
AGAGGTTGAATCTGAAGAGT+TGG - chr2.4:55023459-55023478 None:intergenic 40.0%
GAAAAAGAGGGTTAGGGTTA+GGG - chr2.4:55023391-55023410 None:intergenic 40.0%
GAATGCTTTGGTTAAGGAGT+CGG + chr2.4:55023951-55023970 MS.gene68901:CDS 40.0%
GACACATGACATGCTGAAAT+TGG + chr2.4:55023988-55024007 MS.gene68901:CDS 40.0%
GGTGGAGGAATTAAAGGATT+TGG + chr2.4:55024009-55024028 MS.gene68901:CDS 40.0%
TGAAAAAGAGGGTTAGGGTT+AGG - chr2.4:55023392-55023411 None:intergenic 40.0%
TGCATGCCAAACTATCTGTA+AGG + chr2.4:55024068-55024087 MS.gene68901:CDS 40.0%
TTGGGTCAATCAGTTAGGTT+GGG + chr2.4:55024225-55024244 MS.gene68901:CDS 40.0%
TTTCCTGCAACCCTTCTAAA+AGG + chr2.4:55023663-55023682 MS.gene68901:CDS 40.0%
TTTGGGTCAATCAGTTAGGT+TGG + chr2.4:55024224-55024243 MS.gene68901:CDS 40.0%
! AAGTCGATGCCTTTAAGAAG+TGG + chr2.4:55024110-55024129 MS.gene68901:CDS 40.0%
! AGTCGATGCCTTTAAGAAGT+GGG + chr2.4:55024111-55024130 MS.gene68901:CDS 40.0%
! GCCAAAATTGTTGATGAGGT+AGG - chr2.4:55023492-55023511 None:intergenic 40.0%
! TAGCCATGGTTCCTTTGAAA+TGG - chr2.4:55023642-55023661 None:intergenic 40.0%
! TTCCTGCAACCCTTCTAAAA+GGG + chr2.4:55023664-55023683 MS.gene68901:CDS 40.0%
!! AAATGGGTGATGGAAAGGAA+TGG - chr2.4:55023183-55023202 None:intergenic 40.0%
!! AGAACAAATGGGTGATGGAA+AGG - chr2.4:55023188-55023207 None:intergenic 40.0%
!! AGCTTTTCAGGCACTACAAA+TGG - chr2.4:55024382-55024401 None:intergenic 40.0%
!! AGGCTTGTTTTCAGGATGTA+GGG - chr2.4:55023764-55023783 None:intergenic 40.0%
!! AGGGTTTGGTGAGAAAGTTA+AGG - chr2.4:55023372-55023391 None:intergenic 40.0%
!! CTGAAGAGTTGGTGGAAAAA+TGG - chr2.4:55023448-55023467 None:intergenic 40.0%
AAACTGATGGTCCTTTGGCA+AGG - chr2.4:55024261-55024280 None:intergenic 45.0%
AACTGATGGTCCTTTGGCAA+GGG - chr2.4:55024260-55024279 None:intergenic 45.0%
AAGTCTGGAAGCAGATTGTG+GGG - chr2.4:55023519-55023538 None:intergenic 45.0%
AGACCAACCCCACTTCTTAA+AGG - chr2.4:55024122-55024141 None:intergenic 45.0%
AGCTGAGAACAAATGGGTGA+TGG - chr2.4:55023193-55023212 None:intergenic 45.0%
CAAAGTCTGGAAGCAGATTG+TGG - chr2.4:55023521-55023540 None:intergenic 45.0%
GGTTGAATCTGAAGAGTTGG+TGG - chr2.4:55023456-55023475 None:intergenic 45.0%
TATTGCAATACAGAGGCCCT+TGG - chr2.4:55024313-55024332 None:intergenic 45.0%
TGACATGCTGAAATTGGTGG+AGG + chr2.4:55023994-55024013 MS.gene68901:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG + chr2.4:55024116-55024135 MS.gene68901:CDS 45.0%
! ATTGCTGATTGCCAATGGAG+TGG + chr2.4:55023910-55023929 MS.gene68901:CDS 45.0%
! CGATGTGCATGTTTTGTCCA+TGG - chr2.4:55024488-55024507 None:intergenic 45.0%
! GTCGATGCCTTTAAGAAGTG+GGG + chr2.4:55024112-55024131 MS.gene68901:CDS 45.0%
!! AACCCTTTTAGAAGGGTTGC+AGG - chr2.4:55023669-55023688 None:intergenic 45.0%
!! GAGGCTTGTTTTCAGGATGT+AGG - chr2.4:55023765-55023784 None:intergenic 45.0%
!! GGCTTGTTTTCAGGATGTAG+GGG - chr2.4:55023763-55023782 None:intergenic 45.0%
AGAGGGTTAGGGTTAGGGTT+TGG - chr2.4:55023386-55023405 None:intergenic 50.0%
CGAAAAGAGGGTCCTTCCAA+GGG + chr2.4:55024294-55024313 MS.gene68901:CDS 50.0%
GAGGTAGGAGACTGCAAAAG+AGG - chr2.4:55023477-55023496 None:intergenic 50.0%
GGCATGCAATGCTACACCAA+AGG - chr2.4:55024056-55024075 None:intergenic 50.0%
TCGAAAAGAGGGTCCTTCCA+AGG + chr2.4:55024293-55024312 MS.gene68901:CDS 50.0%
TGCGTATGTGGAGCAAAGTC+TGG - chr2.4:55023534-55023553 None:intergenic 50.0%
!! TGGGTGATGGAAAGGAATGG+AGG - chr2.4:55023180-55023199 None:intergenic 50.0%
CGAGCGTCTCCGAAGAAAGA+TGG - chr2.4:55023872-55023891 None:intergenic 55.0%
GATGCACTTGCCCTTGCCAA+AGG + chr2.4:55024247-55024266 MS.gene68901:CDS 55.0%
GCAATACAGAGGCCCTTGGA+AGG - chr2.4:55024309-55024328 None:intergenic 55.0%
GGGTTGCAGGAAAGTAGCCA+TGG - chr2.4:55023656-55023675 None:intergenic 55.0%
GTTTGAGTCGGCCACTCCAT+TGG - chr2.4:55023924-55023943 None:intergenic 55.0%
TTTCTTCGGAGACGCTCGTG+TGG + chr2.4:55023874-55023893 MS.gene68901:CDS 55.0%
!! GTCAGAGGTAGAAGCGCCTT+TGG - chr2.4:55023717-55023736 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 55023044 55024510 55023044 ID=MS.gene68901
chr2.4 mRNA 55023044 55024510 55023044 ID=MS.gene68901.t1;Parent=MS.gene68901
chr2.4 exon 55023044 55023050 55023044 ID=MS.gene68901.t1.exon1;Parent=MS.gene68901.t1
chr2.4 CDS 55023044 55023050 55023044 ID=cds.MS.gene68901.t1;Parent=MS.gene68901.t1
chr2.4 exon 55023211 55023243 55023211 ID=MS.gene68901.t1.exon2;Parent=MS.gene68901.t1
chr2.4 CDS 55023211 55023243 55023211 ID=cds.MS.gene68901.t1;Parent=MS.gene68901.t1
chr2.4 exon 55023486 55024510 55023486 ID=MS.gene68901.t1.exon3;Parent=MS.gene68901.t1
chr2.4 CDS 55023486 55024510 55023486 ID=cds.MS.gene68901.t1;Parent=MS.gene68901.t1
Gene Sequence

>MS.gene68901

ATGAGGAGTATGCATGAAAGATATAAACGATTTTCATCGGAATATGACTATATGAGCATTTGTTCTCTATTTTGACTAGCAAAGTTGATATTGTCACTATTTCAATTTCATTTTGCTTCTCTAAATACGAGCTCCTCCATTCCTTTCCATCACCCATTTGTTCTCAGCTTCCATATTTCCATTTCTTTTCGAACTAACAGGTAAGTTACTCATTCTGTGCAATCAAATTCCAAGAATTAGGGTTCAAATTCATATGCTTTAACATTAATTTATCATTTTTAAATGTTGAATATCCATCGTCATAGAGCTTTTCTCTATTTCAAGGCCTTAACTTTCTCACCAAACCCTAACCCTAACCCTCTTTTTCAACGTTATTACCCATCATCACCAACATTTTCTCTCCATTTTTCCACCAACTCTTCAGATTCAACCTCTTTTGCAGTCTCCTACCTCATCAACAATTTTGGCTTTTCCCCACAATCTGCTTCCAGACTTTGCTCCACATACGCAATTAGTTTTAAGACTGCCCAAAAACCTGACTCTGTTATCAACTTCTTTAGAAACTATGGTTTTTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACCTCTGACATTGTTAACATTTTCACTAAGAGCCCCTACATCCTGAAAACAAGCCTCAATAATAAAATTATCCAAGCCTATCAATTGATTTATAGATTCTTGCAATCAGACAAGGACATTATTGATTGTGTAATTCGCAATCCATCTTTCTTCGGAGACGCTCGTGTGGTATCTAACATTAGATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGGTTAAGGAGTCGGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAATTAAAGGATTTGGGTTTTGCTACTTCAAAAACAACCTTTGGTGTAGCATTGCATGCCAAACTATCTGTAAGGAAAACATTGTGGGAAGAGAAAGTCGATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCATTTAGAAGGCAACCACATTGTATGTTGGTATCCATTGATAAAATCAATTTAGTGATGAACTTTTGGGTCAATCAGTTAGGTTGGGATGCACTTGCCCTTGCCAAAGGACCATCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAAAAAGGATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAAAAGCTGTTTCTTGATAAGTGTATAAAGGGTATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCGTGA
Protein sequence

>MS.gene68901.t1

MRTSIFPFLFELTVSYLINNFGFSPQSASRLCSTYAISFKTAQKPDSVINFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSKGASTSDIVNIFTKSPYILKTSLNNKIIQAYQLIYRFLQSDKDIIDCVIRNPSFFGDARVVSNIRLLIANGVADSNIKRMLWLRSRAFQTPTHDMLKLVEELKDLGFATSKTTFGVALHAKLSVRKTLWEEKVDAFKKWGWSDEDVLQAFRRQPHCMLVSIDKINLVMNFWVNQLGWDALALAKGPSVFSLSFEKRVLPRASVLQYLLKKGLRKKDASLTCPFVVPEKLFLDKCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS