AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073371

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073371.t1 MTR_2g437020 93.734 383 24 0 1 383 1 383 0.0 731
MS.gene073371.t1 MTR_2g437060 69.114 395 103 5 2 380 4 395 0.0 535
MS.gene073371.t1 MTR_2g437080 66.494 385 117 4 5 382 3 382 0.0 510
MS.gene073371.t1 MTR_2g436440 69.189 370 110 3 15 383 23 389 3.32e-178 501
MS.gene073371.t1 MTR_2g437030 68.108 370 114 3 16 383 102 469 1.96e-172 489
MS.gene073371.t1 MTR_2g436400 64.583 384 110 4 7 374 8 381 2.26e-172 486
MS.gene073371.t1 MTR_2g437040 67.227 357 114 2 25 380 26 380 6.48e-167 472
MS.gene073371.t1 MTR_2g437120 57.065 368 149 7 17 378 27 391 2.69e-133 388
MS.gene073371.t1 MTR_2g437150 54.278 374 157 7 17 378 27 398 7.98e-129 376
MS.gene073371.t1 MTR_2g437170 54.545 374 156 7 17 378 27 398 1.76e-127 373
MS.gene073371.t1 MTR_2g437960 53.100 371 163 4 19 380 28 396 7.04e-126 369
MS.gene073371.t1 MTR_2g437960 52.957 372 164 4 19 381 28 397 8.76e-126 369
MS.gene073371.t1 MTR_4g119550 54.179 347 153 4 35 379 47 389 1.61e-124 365
MS.gene073371.t1 MTR_2g438010 52.066 363 161 5 22 374 48 407 9.77e-123 361
MS.gene073371.t1 MTR_2g437160 52.304 369 166 5 17 378 38 403 6.93e-122 358
MS.gene073371.t1 MTR_2g437200 50.390 385 146 6 27 370 18 398 9.71e-122 358
MS.gene073371.t1 MTR_2g437180 51.058 378 176 6 5 378 16 388 2.20e-121 357
MS.gene073371.t1 MTR_2g437990 51.639 366 165 6 22 378 28 390 1.42e-120 356
MS.gene073371.t1 MTR_2g437260 50.000 354 172 3 24 374 19 370 1.46e-120 355
MS.gene073371.t1 MTR_2g076320 52.316 367 162 5 22 378 28 391 2.25e-120 354
MS.gene073371.t1 MTR_2g437100 54.054 370 152 7 17 370 28 395 1.30e-119 353
MS.gene073371.t1 MTR_2g437130 51.064 376 173 6 7 378 11 379 6.17e-118 348
MS.gene073371.t1 MTR_2g437240 47.962 417 167 8 1 378 1 406 2.64e-114 339
MS.gene073371.t1 MTR_2g436460 49.422 346 169 4 30 373 38 379 2.67e-108 323
MS.gene073371.t1 MTR_2g437940 49.728 368 147 7 17 378 27 362 2.35e-107 320
MS.gene073371.t1 MTR_2g438020 47.619 378 155 5 38 376 70 443 3.88e-107 324
MS.gene073371.t1 MTR_4g119580 45.773 343 179 4 29 368 32 370 2.91e-94 287
MS.gene073371.t1 MTR_2g019840 46.234 385 184 8 9 379 5 380 1.63e-92 283
MS.gene073371.t1 MTR_4g120380 45.994 337 177 3 33 368 38 370 1.42e-89 275
MS.gene073371.t1 MTR_4g120380 47.003 317 164 2 33 349 38 350 6.47e-87 270
MS.gene073371.t1 MTR_2g019810 47.164 335 166 7 40 370 32 359 1.46e-86 267
MS.gene073371.t1 MTR_2g437090 70.000 170 50 1 214 382 1 170 1.11e-83 252
MS.gene073371.t1 MTR_2g436370 58.416 202 61 2 1 187 1 194 9.50e-68 213
MS.gene073371.t1 MTR_2g437160 54.950 202 85 3 181 378 113 312 2.16e-66 213
MS.gene073371.t1 MTR_2g437160 45.614 114 56 3 17 125 38 150 9.82e-19 86.7
MS.gene073371.t1 MTR_3g450500 33.241 361 222 4 19 364 9 365 1.49e-52 179
MS.gene073371.t1 MTR_4g007490 32.530 332 209 7 27 350 25 349 2.80e-43 154
MS.gene073371.t1 MTR_4g119570 37.349 249 120 3 119 366 14 227 3.17e-43 150
MS.gene073371.t1 MTR_8g080990 29.630 297 202 2 72 364 74 367 2.76e-31 122
MS.gene073371.t1 MTR_8g081000 25.656 343 246 3 34 375 50 384 6.80e-28 113
MS.gene073371.t1 MTR_8g081000 25.656 343 246 3 34 375 50 384 8.05e-28 113
MS.gene073371.t1 MTR_8g081000 26.557 305 216 2 72 375 87 384 1.10e-27 113
MS.gene073371.t1 MTR_4g049400 46.923 130 56 4 19 139 25 150 1.96e-27 108
MS.gene073371.t1 MTR_2g436380 70.492 61 18 0 214 274 1 61 2.16e-23 99.0
MS.gene073371.t1 MTR_3g085240 64.000 50 18 0 273 322 176 225 4.54e-15 74.7
MS.gene073371.t1 MTR_3g085240 36.000 150 82 5 14 161 33 170 4.67e-15 74.7
MS.gene073371.t1 MTR_8g012210 22.261 283 198 8 84 355 62 333 8.22e-12 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073371.t1 AT5G07900 32.891 377 239 6 2 367 26 399 2.70e-61 203
MS.gene073371.t1 AT1G21150 30.968 310 207 3 30 335 10 316 4.00e-43 154
MS.gene073371.t1 AT1G21150 32.000 300 197 3 40 335 56 352 6.50e-43 154
MS.gene073371.t1 AT1G21150 32.000 300 197 3 40 335 120 416 2.47e-42 154
MS.gene073371.t1 AT1G61980 29.888 358 200 9 40 355 52 400 2.04e-31 124
MS.gene073371.t1 AT1G61980 29.888 358 200 9 40 355 52 400 2.04e-31 124
MS.gene073371.t1 AT1G61980 29.888 358 200 9 40 355 52 400 2.04e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61970 28.000 350 207 6 41 351 53 396 2.06e-31 124
MS.gene073371.t1 AT1G61990 28.701 331 195 5 41 338 53 375 1.87e-30 120
MS.gene073371.t1 AT1G61990 28.701 331 195 5 41 338 53 375 1.87e-30 120
MS.gene073371.t1 AT5G64950 27.832 309 205 5 30 326 26 328 8.08e-30 119
MS.gene073371.t1 AT1G62120 29.600 375 204 13 7 331 17 381 9.64e-30 119
MS.gene073371.t1 AT3G46950 31.119 286 180 8 7 286 17 291 8.02e-27 111
MS.gene073371.t1 AT1G62085 26.781 407 204 13 8 330 18 414 2.56e-26 110
MS.gene073371.t1 AT1G62085 26.781 407 204 13 8 330 18 414 2.56e-26 110
MS.gene073371.t1 AT5G23930 26.509 381 202 8 40 350 55 427 1.11e-24 105
MS.gene073371.t1 AT1G62010 27.797 295 169 4 75 331 71 359 2.46e-24 103
MS.gene073371.t1 AT1G62110 28.421 285 191 6 7 284 17 295 4.06e-23 100
MS.gene073371.t1 AT1G61960 29.839 248 164 4 41 284 53 294 1.76e-21 95.9
MS.gene073371.t1 AT1G62150 25.581 258 175 4 34 283 52 300 1.22e-19 90.5
MS.gene073371.t1 AT1G79220 23.975 317 220 10 76 380 82 389 1.09e-11 66.2

Find 86 sgRNAs with CRISPR-Local

Find 105 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTATTCAGGCTTGCTTTA+AGG 0.167218 2.4:-54639397 None:intergenic
TTAATTTACTCATGAACTTT+TGG 0.214050 2.4:+54639834 MS.gene073371:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2.4:+54639291 MS.gene073371:CDS
GAAATTGGTGGAGGAATTAA+AGG 0.238950 2.4:+54639631 MS.gene073371:CDS
CTCACCTTCTTTAGAAACTA+TGG 0.240194 2.4:+54639218 MS.gene073371:CDS
GGTGGAGGAATTAAAGGATT+TGG 0.242361 2.4:+54639637 MS.gene073371:CDS
ATTGTAAGTGGAGGAAAGTT+TGG 0.277663 2.4:-54639159 None:intergenic
CAGGCTTGCTTTAAGGATCT+TGG 0.285333 2.4:-54639390 None:intergenic
GTTTCTTGATACATGTATAA+AGG 0.301142 2.4:+54640030 MS.gene073371:CDS
CCATGTTTAACAATATATTA+TGG 0.307920 2.4:+54638985 None:intergenic
AGGCTTGCTTTAAGGATCTT+GGG 0.308598 2.4:-54639389 None:intergenic
GTGGAGGAATTAAAGGATTT+GGG 0.314616 2.4:+54639638 MS.gene073371:CDS
ATTCGCAATCCATCTTTCTT+TGG 0.319814 2.4:+54639488 MS.gene073371:CDS
AAAGAGGGTTAGGGTTTAGA+AGG 0.320241 2.4:-54639022 None:intergenic
AGACCAACCCCACTTCTTAA+AGG 0.324708 2.4:-54639747 None:intergenic
GACACATGACATGCTGAAAT+TGG 0.353711 2.4:+54639616 MS.gene073371:CDS
TACATGTATAAAGGGCATTA+AGG 0.358969 2.4:+54640039 MS.gene073371:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.4:+54639853 MS.gene073371:CDS
TAATGTTGGAAAAGAGGGTT+AGG 0.372467 2.4:-54639032 None:intergenic
ATGTTCTACAAGCGTTTAGA+AGG 0.384175 2.4:+54639777 MS.gene073371:CDS
TTTCTTGATACATGTATAAA+GGG 0.388445 2.4:+54640031 MS.gene073371:CDS
ACTCCTTCAAAATCAACCTT+TGG 0.392434 2.4:+54639665 MS.gene073371:CDS
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2.4:-54639267 None:intergenic
GTCAGAGTTAGAAGCACCTT+TGG 0.395726 2.4:-54639342 None:intergenic
CTGAAGTGTTGGTGCAGAAA+TGG 0.408928 2.4:-54639073 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2.4:+54639953 MS.gene073371:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2.4:-54639252 None:intergenic
AGCTTCTCAGGCACTACAAA+TGG 0.425476 2.4:-54640007 None:intergenic
GAATCTATAAATCAATTGAT+AGG 0.434987 2.4:-54639432 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2.4:+54640069 MS.gene073371:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2.4:+54639909 MS.gene073371:CDS
AAAAGGGTAATGAGTAATGT+TGG 0.457470 2.4:-54639046 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2.4:-54639253 None:intergenic
AATGCTTTGCGTAAGGAGTC+AGG 0.470114 2.4:+54639580 MS.gene073371:CDS
GTATCAAGAAACAGCTTCTC+AGG 0.470819 2.4:-54640019 None:intergenic
GAAAGTTTGGAAGCAGATTG+TGG 0.472463 2.4:-54639146 None:intergenic
CTTGAAACAAACATTGTAAG+TGG 0.473763 2.4:-54639171 None:intergenic
AAGAAGGTGAGAACAGAGTC+AGG 0.478602 2.4:-54639206 None:intergenic
TCCTACCTCATCAACAACTT+TGG 0.478665 2.4:+54639116 MS.gene073371:CDS
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.4:+54639852 MS.gene073371:CDS
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2.4:+54639292 MS.gene073371:CDS
GTGCAGAAATGGAGAGAAAA+GGG 0.484129 2.4:-54639062 None:intergenic
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2.4:-54639549 None:intergenic
AAAGTTTGGAAGCAGATTGT+GGG 0.488544 2.4:-54639145 None:intergenic
GAAACCATAGTTTCTAAAGA+AGG 0.490559 2.4:-54639222 None:intergenic
GGCTTGCTTTAAGGATCTTG+GGG 0.492460 2.4:-54639388 None:intergenic
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2.4:+54639910 MS.gene073371:CDS
AATGAGTAATGTTGGAAAAG+AGG 0.500309 2.4:-54639038 None:intergenic
CCATAATATATTGTTAAACA+TGG 0.502176 2.4:-54638985 None:intergenic
GGTGCAGAAATGGAGAGAAA+AGG 0.506359 2.4:-54639063 None:intergenic
ATGAGTAATGTTGGAAAAGA+GGG 0.517078 2.4:-54639037 None:intergenic
TTAAAAGAATGCTTTGCGTA+AGG 0.521144 2.4:+54639573 MS.gene073371:CDS
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2.4:+54639921 MS.gene073371:CDS
ATTATATTGCTGATTGCCAA+TGG 0.523498 2.4:+54639533 MS.gene073371:CDS
CGAGCGTCTCCAAAGAAAGA+TGG 0.526080 2.4:-54639497 None:intergenic
AAACTGCTGGTCCTTTGGCA+AGG 0.527369 2.4:-54639886 None:intergenic
AAGAGGGTTAGGGTTTAGAA+GGG 0.536351 2.4:-54639021 None:intergenic
ACTACGTCTTACCATTTCAA+AGG 0.541064 2.4:+54639256 MS.gene073371:CDS
AGTCGATGCCTTTAAGAAGT+GGG 0.559365 2.4:+54639739 MS.gene073371:CDS
AACTGCTGGTCCTTTGGCAA+GGG 0.562420 2.4:-54639885 None:intergenic
GAGGTAGGAGACTGCAAAAG+AGG 0.562960 2.4:-54639102 None:intergenic
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2.4:+54639875 MS.gene073371:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2.4:-54639938 None:intergenic
AGAGGTCGAATCTGAAGTGT+TGG 0.569312 2.4:-54639084 None:intergenic
AATGTTGGAAAAGAGGGTTA+GGG 0.572620 2.4:-54639031 None:intergenic
GGCTAGCAATGCTACACCAA+AGG 0.575700 2.4:-54639681 None:intergenic
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2.4:+54639922 MS.gene073371:CDS
AAGTCGATGCCTTTAAGAAG+TGG 0.577016 2.4:+54639738 MS.gene073371:CDS
TAGATTCTTGCAATCAGACA+AGG 0.583953 2.4:+54639448 MS.gene073371:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2.4:-54639934 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.598873 2.4:+54639744 MS.gene073371:CDS
GCCAAAGTTGTTGATGAGGT+AGG 0.602091 2.4:-54639117 None:intergenic
GTCGATGCCTTTAAGAAGTG+GGG 0.602513 2.4:+54639740 MS.gene073371:CDS
ACATGACATGCTGAAATTGG+TGG 0.610610 2.4:+54639619 MS.gene073371:CDS
AAGTTTGGAAGCAGATTGTG+GGG 0.614108 2.4:-54639144 None:intergenic
TGACATGCTGAAATTGGTGG+AGG 0.631062 2.4:+54639622 MS.gene073371:CDS
AAAAGCCAAAGTTGTTGATG+AGG 0.632517 2.4:-54639121 None:intergenic
TTTCTTTGGAGACGCTCGTG+TGG 0.632941 2.4:+54639502 MS.gene073371:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2.4:+54640096 MS.gene073371:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2.4:-54639614 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2.4:-54639281 None:intergenic
GAAACAAACATTGTAAGTGG+AGG 0.650981 2.4:-54639168 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2.4:+54639264 MS.gene073371:CDS
ATGGATGTTAACATACAATG+AGG 0.655070 2.4:-54639803 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.669284 2.4:+54639538 MS.gene073371:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2.4:-54639945 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CTTTGATAATTTTATTATTC+AGG - chr2.4:54639412-54639431 None:intergenic 15.0%
!!! GAGTAAATTAATTTTATCAA+TGG - chr2.4:54639825-54639844 None:intergenic 15.0%
!! GAATCTATAAATCAATTGAT+AGG - chr2.4:54639435-54639454 None:intergenic 20.0%
!! TTATCTATTAAAGTTGTATG+AGG + chr2.4:54640069-54640088 MS.gene073371:CDS 20.0%
!! TTTCTTGATACATGTATAAA+GGG + chr2.4:54640031-54640050 MS.gene073371:CDS 20.0%
!!! TAATTTACTCATGAACTTTT+GGG + chr2.4:54639835-54639854 MS.gene073371:CDS 20.0%
!!! TTAATTTACTCATGAACTTT+TGG + chr2.4:54639834-54639853 MS.gene073371:CDS 20.0%
! AAACTTAAAGAAAAAACTGC+TGG - chr2.4:54639902-54639921 None:intergenic 25.0%
! ATCTGTAAGAAAAACATTGT+GGG + chr2.4:54639709-54639728 MS.gene073371:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.4:54639323-54639342 None:intergenic 25.0%
! GTTTCTTGATACATGTATAA+AGG + chr2.4:54640030-54640049 MS.gene073371:CDS 25.0%
! TATCTGTAAGAAAAACATTG+TGG + chr2.4:54639708-54639727 MS.gene073371:CDS 25.0%
!! CATTCTTTTAATGTTTGAGT+CGG - chr2.4:54639564-54639583 None:intergenic 25.0%
!! TGTTTTTCTTACAGATAGTT+TGG - chr2.4:54639705-54639724 None:intergenic 25.0%
!! TTCTTTAAGTTTCGAAAAGA+GGG + chr2.4:54639910-54639929 MS.gene073371:CDS 25.0%
!! TTTCTTTAAGTTTCGAAAAG+AGG + chr2.4:54639909-54639928 MS.gene073371:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.4:54639326-54639345 MS.gene073371:CDS 25.0%
AAAAGGGTAATGAGTAATGT+TGG - chr2.4:54639049-54639068 None:intergenic 30.0%
AATGAGTAATGTTGGAAAAG+AGG - chr2.4:54639041-54639060 None:intergenic 30.0%
ATGAGTAATGTTGGAAAAGA+GGG - chr2.4:54639040-54639059 None:intergenic 30.0%
ATGGATGTTAACATACAATG+AGG - chr2.4:54639806-54639825 None:intergenic 30.0%
CTTGAAACAAACATTGTAAG+TGG - chr2.4:54639174-54639193 None:intergenic 30.0%
TCTGTAAGAAAAACATTGTG+GGG + chr2.4:54639710-54639729 MS.gene073371:CDS 30.0%
TTAAAAGAATGCTTTGCGTA+AGG + chr2.4:54639573-54639592 MS.gene073371:CDS 30.0%
! ATTATATTGCTGATTGCCAA+TGG + chr2.4:54639533-54639552 MS.gene073371:CDS 30.0%
! GAAACCATAGTTTCTAAAGA+AGG - chr2.4:54639225-54639244 None:intergenic 30.0%
! TATTATTCAGGCTTGCTTTA+AGG - chr2.4:54639400-54639419 None:intergenic 30.0%
! TTGCAATATCTTCTGAAGAA+AGG + chr2.4:54639953-54639972 MS.gene073371:CDS 30.0%
!! TACATGTATAAAGGGCATTA+AGG + chr2.4:54640039-54640058 MS.gene073371:CDS 30.0%
AAAAGCCAAAGTTGTTGATG+AGG - chr2.4:54639124-54639143 None:intergenic 35.0%
AAAGTTTGGAAGCAGATTGT+GGG - chr2.4:54639148-54639167 None:intergenic 35.0%
AATGTTGGAAAAGAGGGTTA+GGG - chr2.4:54639034-54639053 None:intergenic 35.0%
AATTTCAGCATGTCATGTGT+CGG - chr2.4:54639617-54639636 None:intergenic 35.0%
ACTACGTCTTACCATTTCAA+AGG + chr2.4:54639256-54639275 MS.gene073371:CDS 35.0%
ACTCCTTCAAAATCAACCTT+TGG + chr2.4:54639665-54639684 MS.gene073371:CDS 35.0%
ATGTTCTACAAGCGTTTAGA+AGG + chr2.4:54639777-54639796 MS.gene073371:CDS 35.0%
ATTCGCAATCCATCTTTCTT+TGG + chr2.4:54639488-54639507 MS.gene073371:CDS 35.0%
ATTGTAAGTGGAGGAAAGTT+TGG - chr2.4:54639162-54639181 None:intergenic 35.0%
CAGAAGATATTGCAATACAG+AGG - chr2.4:54639948-54639967 None:intergenic 35.0%
GAAACAAACATTGTAAGTGG+AGG - chr2.4:54639171-54639190 None:intergenic 35.0%
GAAATTGGTGGAGGAATTAA+AGG + chr2.4:54639631-54639650 MS.gene073371:CDS 35.0%
GTGGAGGAATTAAAGGATTT+GGG + chr2.4:54639638-54639657 MS.gene073371:CDS 35.0%
TAATGTTGGAAAAGAGGGTT+AGG - chr2.4:54639035-54639054 None:intergenic 35.0%
TAGATTCTTGCAATCAGACA+AGG + chr2.4:54639448-54639467 MS.gene073371:CDS 35.0%
TGAAATGGTAAGACGTAGTT+GGG - chr2.4:54639255-54639274 None:intergenic 35.0%
TTACCATTTCAAAGGAACCA+TGG + chr2.4:54639264-54639283 MS.gene073371:CDS 35.0%
TTGAAATGGTAAGACGTAGT+TGG - chr2.4:54639256-54639275 None:intergenic 35.0%
! CTCACCTTCTTTAGAAACTA+TGG + chr2.4:54639218-54639237 MS.gene073371:CDS 35.0%
! TTTGGCAAAACCCTTTTAGA+AGG - chr2.4:54639305-54639324 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG + chr2.4:54639848-54639867 MS.gene073371:CDS 35.0%
!! ACACCAAAGGTTGATTTTGA+AGG - chr2.4:54639671-54639690 None:intergenic 35.0%
!! TTGGCAAAACCCTTTTAGAA+GGG - chr2.4:54639304-54639323 None:intergenic 35.0%
AAAGAGGGTTAGGGTTTAGA+AGG - chr2.4:54639025-54639044 None:intergenic 40.0%
AAGAGGGTTAGGGTTTAGAA+GGG - chr2.4:54639024-54639043 None:intergenic 40.0%
AAGTTTGGAAGCAGATTGTG+GGG - chr2.4:54639147-54639166 None:intergenic 40.0%
ACATGACATGCTGAAATTGG+TGG + chr2.4:54639619-54639638 MS.gene073371:CDS 40.0%
ACTCAATCTTGCTTACACCA+TGG + chr2.4:54640096-54640115 MS.gene073371:CDS 40.0%
AGAAAAAACTGCTGGTCCTT+TGG - chr2.4:54639894-54639913 None:intergenic 40.0%
GAAAGTTTGGAAGCAGATTG+TGG - chr2.4:54639149-54639168 None:intergenic 40.0%
GACACATGACATGCTGAAAT+TGG + chr2.4:54639616-54639635 MS.gene073371:CDS 40.0%
GGTGGAGGAATTAAAGGATT+TGG + chr2.4:54639637-54639656 MS.gene073371:CDS 40.0%
GTATCAAGAAACAGCTTCTC+AGG - chr2.4:54640022-54640041 None:intergenic 40.0%
GTGCAGAAATGGAGAGAAAA+GGG - chr2.4:54639065-54639084 None:intergenic 40.0%
TCCTACCTCATCAACAACTT+TGG + chr2.4:54639116-54639135 MS.gene073371:CDS 40.0%
TTTCCTGCAACCCTTCTAAA+AGG + chr2.4:54639291-54639310 MS.gene073371:CDS 40.0%
! AAGTCGATGCCTTTAAGAAG+TGG + chr2.4:54639738-54639757 MS.gene073371:CDS 40.0%
! AGTCGATGCCTTTAAGAAGT+GGG + chr2.4:54639739-54639758 MS.gene073371:CDS 40.0%
! GAGAACAGAGTCAGGTTTTT+GGG - chr2.4:54639201-54639220 None:intergenic 40.0%
! TAGCCATGGTTCCTTTGAAA+TGG - chr2.4:54639270-54639289 None:intergenic 40.0%
! TGAGAACAGAGTCAGGTTTT+TGG - chr2.4:54639202-54639221 None:intergenic 40.0%
! TGATGTGCATGTTTTGTCCA+TGG - chr2.4:54640116-54640135 None:intergenic 40.0%
! TTCCTGCAACCCTTCTAAAA+GGG + chr2.4:54639292-54639311 MS.gene073371:CDS 40.0%
!! AGGCTTGCTTTAAGGATCTT+GGG - chr2.4:54639392-54639411 None:intergenic 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG + chr2.4:54639847-54639866 MS.gene073371:CDS 40.0%
AAGAAGGTGAGAACAGAGTC+AGG - chr2.4:54639209-54639228 None:intergenic 45.0%
AATGCTTTGCGTAAGGAGTC+AGG + chr2.4:54639580-54639599 MS.gene073371:CDS 45.0%
AGACCAACCCCACTTCTTAA+AGG - chr2.4:54639750-54639769 None:intergenic 45.0%
AGCTTCTCAGGCACTACAAA+TGG - chr2.4:54640010-54640029 None:intergenic 45.0%
GGTGCAGAAATGGAGAGAAA+AGG - chr2.4:54639066-54639085 None:intergenic 45.0%
TATTGCAATACAGAGGCCCT+TGG - chr2.4:54639941-54639960 None:intergenic 45.0%
TGACATGCTGAAATTGGTGG+AGG + chr2.4:54639622-54639641 MS.gene073371:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG + chr2.4:54639853-54639872 MS.gene073371:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG + chr2.4:54639852-54639871 MS.gene073371:CDS 45.0%
! AGAGGTCGAATCTGAAGTGT+TGG - chr2.4:54639087-54639106 None:intergenic 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG + chr2.4:54639744-54639763 MS.gene073371:CDS 45.0%
! ATTGCTGATTGCCAATGGAG+TGG + chr2.4:54639538-54639557 MS.gene073371:CDS 45.0%
! GCCAAAGTTGTTGATGAGGT+AGG - chr2.4:54639120-54639139 None:intergenic 45.0%
! GTCGATGCCTTTAAGAAGTG+GGG + chr2.4:54639740-54639759 MS.gene073371:CDS 45.0%
!! AACCCTTTTAGAAGGGTTGC+AGG - chr2.4:54639297-54639316 None:intergenic 45.0%
!! CAGGCTTGCTTTAAGGATCT+TGG - chr2.4:54639393-54639412 None:intergenic 45.0%
!! CTGAAGTGTTGGTGCAGAAA+TGG - chr2.4:54639076-54639095 None:intergenic 45.0%
!! GGCTTGCTTTAAGGATCTTG+GGG - chr2.4:54639391-54639410 None:intergenic 45.0%
!! GTCAGAGTTAGAAGCACCTT+TGG - chr2.4:54639345-54639364 None:intergenic 45.0%
AAACTGCTGGTCCTTTGGCA+AGG - chr2.4:54639889-54639908 None:intergenic 50.0%
AACTGCTGGTCCTTTGGCAA+GGG - chr2.4:54639888-54639907 None:intergenic 50.0%
CGAAAAGAGGGTCCTTCCAA+GGG + chr2.4:54639922-54639941 MS.gene073371:CDS 50.0%
CGAGCGTCTCCAAAGAAAGA+TGG - chr2.4:54639500-54639519 None:intergenic 50.0%
GAGGTAGGAGACTGCAAAAG+AGG - chr2.4:54639105-54639124 None:intergenic 50.0%
GGCTAGCAATGCTACACCAA+AGG - chr2.4:54639684-54639703 None:intergenic 50.0%
TCGAAAAGAGGGTCCTTCCA+AGG + chr2.4:54639921-54639940 MS.gene073371:CDS 50.0%
! TTTCTTTGGAGACGCTCGTG+TGG + chr2.4:54639502-54639521 MS.gene073371:CDS 50.0%
GATGCACTTGCCCTTGCCAA+AGG + chr2.4:54639875-54639894 MS.gene073371:CDS 55.0%
GCAATACAGAGGCCCTTGGA+AGG - chr2.4:54639937-54639956 None:intergenic 55.0%
GGGTTGCAGGAAAGTAGCCA+TGG - chr2.4:54639284-54639303 None:intergenic 55.0%
GTTTGAGTCGGCCACTCCAT+TGG - chr2.4:54639552-54639571 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 54638987 54640138 54638987 ID=MS.gene073371
chr2.4 mRNA 54638987 54640138 54638987 ID=MS.gene073371.t1;Parent=MS.gene073371
chr2.4 exon 54638987 54640138 54638987 ID=MS.gene073371.t1.exon1;Parent=MS.gene073371.t1
chr2.4 CDS 54638987 54640138 54638987 ID=cds.MS.gene073371.t1;Parent=MS.gene073371.t1
Gene Sequence

>MS.gene073371

ATGTTTAACAATATATTATGGCGACACAGAACAACCCTTCTAAACCCTAACCCTCTTTTCCAACATTACTCATTACCCTTTTCTCTCCATTTCTGCACCAACACTTCAGATTCGACCTCTTTTGCAGTCTCCTACCTCATCAACAACTTTGGCTTTTCCCCACAATCTGCTTCCAAACTTTCCTCCACTTACAATGTTTGTTTCAAGAATGCCCAAAAACCTGACTCTGTTCTCACCTTCTTTAGAAACTATGGTTTCTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTAACTCTGACATTGTTAACATTGTCACTAAGAACCCCAAGATCCTTAAAGCAAGCCTGAATAATAAAATTATCAAAGCCTATCAATTGATTTATAGATTCTTGCAATCAGACAAGGTCATTATTGATTGTGTTATTCGCAATCCATCTTTCTTTGGAGACGCTCGTGTGGTATCTAACATTATATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGCGTAAGGAGTCAGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAATTAAAGGATTTGGGTTTTACTCCTTCAAAATCAACCTTTGGTGTAGCATTGCTAGCCAAACTATCTGTAAGAAAAACATTGTGGGGAGAGAAAGTCGATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCGTTTAGAAGGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTACTCATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCACTTGCCCTTGCCAAAGGACCAGCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAACAAGAATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAGAAGCTGTTTCTTGATACATGTATAAAGGGCATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCATGA
Protein sequence

>MS.gene073371.t1

MFNNILWRHRTTLLNPNPLFQHYSLPFSLHFCTNTSDSTSFAVSYLINNFGFSPQSASKLSSTYNVCFKNAQKPDSVLTFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSKGASNSDIVNIVTKNPKILKASLNNKIIKAYQLIYRFLQSDKVIIDCVIRNPSFFGDARVVSNIILLIANGVADSNIKRMLCVRSQAFQTPTHDMLKLVEELKDLGFTPSKSTFGVALLAKLSVRKTLWGEKVDAFKKWGWSDEDVLQAFRRQPHCMLTSIDKINLLMNFWVNQLGWDALALAKGPAVFSLSFEKRVLPRASVLQYLLKKGLRNKNASLTCPFVVPEKLFLDTCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS