AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004543

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004543.t1 MTR_2g437020 88.172 372 41 1 23 394 15 383 0.0 659
MS.gene004543.t1 MTR_2g437060 67.677 396 115 5 3 391 6 395 0.0 523
MS.gene004543.t1 MTR_2g437030 66.582 395 127 3 1 394 79 469 0.0 514
MS.gene004543.t1 MTR_2g437080 65.570 395 121 4 1 393 1 382 0.0 513
MS.gene004543.t1 MTR_2g436440 63.291 395 138 4 1 394 1 389 1.41e-170 482
MS.gene004543.t1 MTR_2g436400 63.377 385 124 4 5 385 10 381 9.17e-169 477
MS.gene004543.t1 MTR_2g437040 62.341 393 133 5 1 391 1 380 3.35e-161 458
MS.gene004543.t1 MTR_2g437120 54.717 371 156 7 25 389 27 391 4.36e-127 372
MS.gene004543.t1 MTR_4g119550 54.179 347 153 4 46 390 47 389 2.60e-124 364
MS.gene004543.t1 MTR_2g437960 51.407 391 181 6 7 391 9 396 1.35e-123 363
MS.gene004543.t1 MTR_2g437960 51.407 391 181 6 7 391 9 396 2.68e-123 363
MS.gene004543.t1 MTR_2g437160 52.033 369 170 5 25 389 38 403 3.07e-122 360
MS.gene004543.t1 MTR_2g437170 52.000 375 167 6 25 389 27 398 6.71e-122 359
MS.gene004543.t1 MTR_2g437150 52.394 376 164 7 25 389 27 398 7.73e-122 358
MS.gene004543.t1 MTR_2g438010 52.991 351 159 3 39 385 59 407 2.55e-121 358
MS.gene004543.t1 MTR_2g437200 50.386 389 148 6 34 381 14 398 7.67e-121 356
MS.gene004543.t1 MTR_2g437990 52.542 354 163 4 39 389 39 390 2.66e-119 353
MS.gene004543.t1 MTR_2g076320 51.831 355 165 3 39 389 39 391 1.06e-117 348
MS.gene004543.t1 MTR_2g437130 49.745 392 174 6 1 389 8 379 1.08e-117 347
MS.gene004543.t1 MTR_2g437260 49.858 351 171 3 38 385 22 370 6.95e-117 345
MS.gene004543.t1 MTR_2g437180 50.266 376 179 5 17 389 18 388 7.25e-117 346
MS.gene004543.t1 MTR_2g437240 49.219 384 152 5 45 389 27 406 9.45e-115 341
MS.gene004543.t1 MTR_2g437100 52.688 372 157 7 25 381 28 395 3.07e-114 339
MS.gene004543.t1 MTR_2g438020 48.413 378 152 5 49 387 70 443 1.35e-107 325
MS.gene004543.t1 MTR_2g436460 48.266 346 173 4 41 384 38 379 1.23e-106 319
MS.gene004543.t1 MTR_2g437940 48.780 369 152 6 25 389 27 362 2.06e-106 318
MS.gene004543.t1 MTR_4g119580 43.717 382 200 5 1 379 1 370 1.88e-95 290
MS.gene004543.t1 MTR_4g120380 44.591 379 195 6 5 379 3 370 1.03e-90 278
MS.gene004543.t1 MTR_2g019840 45.869 351 181 6 43 390 36 380 1.75e-89 275
MS.gene004543.t1 MTR_4g120380 45.404 359 182 5 5 360 3 350 9.02e-88 272
MS.gene004543.t1 MTR_2g437090 72.353 170 46 1 225 393 1 170 2.27e-84 254
MS.gene004543.t1 MTR_2g019810 45.672 335 171 7 51 381 32 359 3.37e-84 261
MS.gene004543.t1 MTR_2g437160 55.446 202 84 3 192 389 113 312 3.82e-66 213
MS.gene004543.t1 MTR_2g437160 47.475 99 50 2 25 122 38 135 1.12e-19 89.4
MS.gene004543.t1 MTR_2g436370 53.807 197 77 2 5 198 9 194 5.14e-61 196
MS.gene004543.t1 MTR_3g450500 31.831 355 227 4 27 375 20 365 1.93e-53 182
MS.gene004543.t1 MTR_4g007490 30.588 340 229 4 30 365 17 353 3.22e-44 157
MS.gene004543.t1 MTR_4g119570 37.052 251 122 3 130 379 14 229 5.08e-42 147
MS.gene004543.t1 MTR_8g080990 28.783 337 232 3 43 375 35 367 1.58e-32 126
MS.gene004543.t1 MTR_8g081000 26.531 343 243 3 45 386 50 384 1.27e-31 124
MS.gene004543.t1 MTR_8g081000 26.531 343 243 3 45 386 50 384 1.69e-31 124
MS.gene004543.t1 MTR_8g081000 26.531 343 243 3 45 386 50 384 3.05e-31 123
MS.gene004543.t1 MTR_4g049400 46.154 130 65 2 25 150 22 150 3.84e-27 108
MS.gene004543.t1 MTR_2g436380 68.852 61 19 0 225 285 1 61 8.06e-21 92.0
MS.gene004543.t1 MTR_3g085240 44.944 89 32 1 284 372 176 247 6.07e-16 77.4
MS.gene004543.t1 MTR_3g085240 34.132 167 91 5 8 173 23 171 4.49e-14 72.0
MS.gene004543.t1 MTR_8g012210 22.180 266 192 5 95 355 62 317 1.12e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004543.t1 AT5G07900 34.551 356 218 7 34 378 48 399 2.10e-63 209
MS.gene004543.t1 AT1G21150 30.645 310 208 3 41 346 10 316 1.09e-42 153
MS.gene004543.t1 AT1G21150 31.667 300 198 3 51 346 56 352 1.79e-42 153
MS.gene004543.t1 AT1G21150 31.667 300 198 3 51 346 120 416 1.09e-41 152
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT1G61970 27.298 359 210 9 49 365 50 399 1.97e-30 121
MS.gene004543.t1 AT5G64950 27.832 309 205 5 41 337 26 328 2.69e-30 120
MS.gene004543.t1 AT1G61980 28.212 358 206 9 51 366 52 400 4.32e-29 117
MS.gene004543.t1 AT1G61980 28.212 358 206 9 51 366 52 400 4.32e-29 117
MS.gene004543.t1 AT1G61980 28.212 358 206 9 51 366 52 400 4.32e-29 117
MS.gene004543.t1 AT1G61990 28.446 341 183 8 52 349 53 375 5.75e-29 117
MS.gene004543.t1 AT1G61990 28.446 341 183 8 52 349 53 375 5.75e-29 117
MS.gene004543.t1 AT3G46950 30.325 277 176 8 27 297 26 291 1.19e-27 114
MS.gene004543.t1 AT1G62120 27.473 364 208 10 27 342 26 381 2.95e-26 109
MS.gene004543.t1 AT1G62010 26.935 323 186 6 64 342 43 359 3.08e-23 100
MS.gene004543.t1 AT1G62085 25.377 398 211 11 24 341 23 414 1.64e-22 99.4
MS.gene004543.t1 AT1G62085 25.377 398 211 11 24 341 23 414 1.64e-22 99.4
MS.gene004543.t1 AT5G23930 25.118 422 227 11 23 363 20 433 1.72e-21 96.3
MS.gene004543.t1 AT1G61960 28.235 255 173 4 45 295 46 294 3.39e-20 92.4
MS.gene004543.t1 AT1G62110 26.374 273 189 5 28 295 30 295 1.16e-18 87.8
MS.gene004543.t1 AT1G62150 24.206 252 186 3 45 294 52 300 1.13e-16 82.0

Find 81 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.173604 2.1:+56938351 MS.gene004543:CDS
TTTCCTGCAACCCTTCTAAA+AGG 0.220666 2.1:+56938526 MS.gene004543:CDS
TTAATTTAGTGATGAACTTT+TGG 0.234643 2.1:+56939069 MS.gene004543:CDS
GAAATTGGTGGAGGAATTAA+AGG 0.238950 2.1:+56938866 MS.gene004543:CDS
GGTGGAGGAATTAAAGGATT+TGG 0.242361 2.1:+56938872 MS.gene004543:CDS
ATCAACTTCTTTAGAAACTA+TGG 0.267482 2.1:+56938453 MS.gene004543:CDS
GTGGAGGAATTAAAGGATTT+GGG 0.314616 2.1:+56938873 MS.gene004543:CDS
AGACCAACCCCACTTCTTAA+AGG 0.324708 2.1:-56938982 None:intergenic
GTTTCTTGATAAGTGTATAA+AGG 0.325915 2.1:+56939265 MS.gene004543:CDS
TTAAAAGAATGCTTTGGTTA+AGG 0.353006 2.1:+56938808 MS.gene004543:CDS
GACACATGACATGCTGAAAT+TGG 0.353711 2.1:+56938851 MS.gene004543:CDS
TAGCCATGGTTCCTTTGAAA+TGG 0.395647 2.1:-56938502 None:intergenic
TTTCTTGATAAGTGTATAAA+GGG 0.396797 2.1:+56939266 MS.gene004543:CDS
ATGTTCTACAAGCATTTAGA+AGG 0.400394 2.1:+56939012 MS.gene004543:CDS
AGAGGGTTAGGGTTAGGGTT+TGG 0.404880 2.1:-56938246 None:intergenic
GTCAGAGGTAGAAGCGCCTT+TGG 0.405353 2.1:-56938577 None:intergenic
GAATCTATAAATCAATTGAT+AGG 0.409000 2.1:-56938667 None:intergenic
TGCGTATGTGGAGCAAAGTC+TGG 0.409089 2.1:-56938394 None:intergenic
TTGCAATATCTTCTGAAGAA+AGG 0.412458 2.1:+56939188 MS.gene004543:CDS
TGAAATGGTAAGACGTAGTT+GGG 0.418042 2.1:-56938487 None:intergenic
TAAGTGTATAAAGGGTATTA+AGG 0.419835 2.1:+56939274 MS.gene004543:CDS
AGAGAAAATGTTGGTGATGA+TGG 0.427085 2.1:-56938285 None:intergenic
ATTCGCAATCCATCTTTCTT+CGG 0.435209 2.1:+56938723 MS.gene004543:CDS
CAAACATTAAAAGAATGCTT+TGG 0.436839 2.1:+56938802 MS.gene004543:CDS
CTTAAAACTAATTGCGTATG+TGG 0.438575 2.1:-56938406 None:intergenic
TTATCTATTAAAGTTGTATG+AGG 0.446208 2.1:+56939304 MS.gene004543:CDS
TTGGGTCAATCAGTTAGGTT+GGG 0.447898 2.1:+56939088 MS.gene004543:CDS
TTTCTTTAAGTTTCGAAAAG+AGG 0.455763 2.1:+56939144 MS.gene004543:CDS
TATAAATCAATTGATAGGCT+TGG 0.456794 2.1:-56938662 None:intergenic
AAGAAGTTGATAACAGAGTC+AGG 0.461266 2.1:-56938441 None:intergenic
TTGAAATGGTAAGACGTAGT+TGG 0.462482 2.1:-56938488 None:intergenic
TTTGGGTCAATCAGTTAGGT+TGG 0.464253 2.1:+56939087 MS.gene004543:CDS
AATGCTTTGGTTAAGGAGTC+GGG 0.471203 2.1:+56938815 MS.gene004543:CDS
TTCCTGCAACCCTTCTAAAA+GGG 0.482738 2.1:+56938527 MS.gene004543:CDS
GTTTGAGTCGGCCACTCCAT+TGG 0.486888 2.1:-56938784 None:intergenic
GAATGCTTTGGTTAAGGAGT+CGG 0.487572 2.1:+56938814 MS.gene004543:CDS
ATCTGTAAGGAAAACATTGT+GGG 0.488825 2.1:+56938944 MS.gene004543:CDS
TTCTTTAAGTTTCGAAAAGA+GGG 0.495393 2.1:+56939145 MS.gene004543:CDS
AAAGTCTGGAAGCAGATTGT+GGG 0.509506 2.1:-56938380 None:intergenic
AGGGTTTGGTGAGAAAGTTA+AGG 0.510973 2.1:-56938232 None:intergenic
CGAGCGTCTCCGAAGAAAGA+TGG 0.514763 2.1:-56938732 None:intergenic
TGCATGCCAAACTATCTGTA+AGG 0.519763 2.1:+56938931 MS.gene004543:CDS
CAAAGTCTGGAAGCAGATTG+TGG 0.519859 2.1:-56938381 None:intergenic
TCGAAAAGAGGGTCCTTCCA+AGG 0.522254 2.1:+56939156 MS.gene004543:CDS
GAGGTAGGAGACGGCAAAAG+AGG 0.528049 2.1:-56938337 None:intergenic
GGTTGAATCTGAAGAGTTGG+TGG 0.537307 2.1:-56938316 None:intergenic
ACTACGTCTTACCATTTCAA+AGG 0.541064 2.1:+56938491 MS.gene004543:CDS
ATTAGATTGCTGATTGCCAA+TGG 0.546889 2.1:+56938768 MS.gene004543:CDS
AAACTGATGGTCCTTTGGCA+AGG 0.547751 2.1:-56939121 None:intergenic
AGAGGTTGAATCTGAAGAGT+TGG 0.550770 2.1:-56938319 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.556947 2.1:+56938979 MS.gene004543:CDS
GATGCACTTGCCCTTGCCAA+AGG 0.563336 2.1:+56939110 MS.gene004543:CDS
AGTCCATGCCTTTAAGAAGT+GGG 0.564002 2.1:+56938974 MS.gene004543:CDS
TATTGCAATACAGAGGCCCT+TGG 0.565124 2.1:-56939173 None:intergenic
GAGAAAATGTTGGTGATGAT+GGG 0.566239 2.1:-56938284 None:intergenic
AACTGATGGTCCTTTGGCAA+GGG 0.571808 2.1:-56939120 None:intergenic
CGAAAAGAGGGTCCTTCCAA+GGG 0.576545 2.1:+56939157 MS.gene004543:CDS
TATCTGTAAGGAAAACATTG+TGG 0.577111 2.1:+56938943 MS.gene004543:CDS
GCCAAAATTGTTGATGAGGT+AGG 0.577466 2.1:-56938352 None:intergenic
AACCCCACTTCTTAAAGGCA+TGG 0.577614 2.1:-56938977 None:intergenic
AGAGAAAAGCTCTATGACGA+TGG 0.581395 2.1:-56938200 None:intergenic
AAGTCCATGCCTTTAAGAAG+TGG 0.581654 2.1:+56938973 MS.gene004543:CDS
AAGGCAACCACATTGTATGT+TGG 0.594814 2.1:+56939031 MS.gene004543:CDS
GCAATACAGAGGCCCTTGGA+AGG 0.596897 2.1:-56939169 None:intergenic
AAAAGCCAAAATTGTTGATG+AGG 0.598384 2.1:-56938356 None:intergenic
GTCCATGCCTTTAAGAAGTG+GGG 0.602513 2.1:+56938975 MS.gene004543:CDS
GGCATGCAATGCTACACCAA+AGG 0.603475 2.1:-56938916 None:intergenic
TAGATTCATGCAATCAGACA+AGG 0.605554 2.1:+56938683 MS.gene004543:CDS
GAAAATGCTAACAATGTCAG+AGG 0.609432 2.1:-56938592 None:intergenic
ACATGACATGCTGAAATTGG+TGG 0.610610 2.1:+56938854 MS.gene004543:CDS
TTTCTTCGGAGACGCTCGTG+TGG 0.619956 2.1:+56938737 MS.gene004543:CDS
ATTGTTGATGAGGTAGGAGA+CGG 0.624106 2.1:-56938346 None:intergenic
TGACATGCTGAAATTGGTGG+AGG 0.631062 2.1:+56938857 MS.gene004543:CDS
ACTCAATCTTGCTTACACCA+TGG 0.635583 2.1:+56939331 MS.gene004543:CDS
AATTTCAGCATGTCATGTGT+CGG 0.639746 2.1:-56938849 None:intergenic
GGGTTGCAGGAAAGTAGCCA+TGG 0.641042 2.1:-56938516 None:intergenic
TTACCATTTCAAAGGAACCA+TGG 0.651356 2.1:+56938499 MS.gene004543:CDS
CAGAAGATATTGCAATACAG+AGG 0.669772 2.1:-56939180 None:intergenic
AAGTCTGGAAGCAGATTGTG+GGG 0.672466 2.1:-56938379 None:intergenic
ATTGCTGATTGCCAATGGAG+TGG 0.672936 2.1:+56938773 MS.gene004543:CDS
ATGGATACCAACATACAATG+TGG 0.711327 2.1:-56939038 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG - chr2.1:56939060-56939079 None:intergenic 15.0%
!! AAACTTAAAGAAAAAACTGA+TGG - chr2.1:56939137-56939156 None:intergenic 20.0%
!! GAATCTATAAATCAATTGAT+AGG - chr2.1:56938670-56938689 None:intergenic 20.0%
!! TTATCTATTAAAGTTGTATG+AGG + chr2.1:56939304-56939323 MS.gene004543:CDS 20.0%
!! TTTCTTGATAAGTGTATAAA+GGG + chr2.1:56939266-56939285 MS.gene004543:CDS 20.0%
!!! CTTGGATAATTTTATTATTG+AGG - chr2.1:56938647-56938666 None:intergenic 20.0%
!!! TAATTTAGTGATGAACTTTT+GGG + chr2.1:56939070-56939089 MS.gene004543:CDS 20.0%
!!! TTAATTTAGTGATGAACTTT+TGG + chr2.1:56939069-56939088 MS.gene004543:CDS 20.0%
! CAAACATTAAAAGAATGCTT+TGG + chr2.1:56938802-56938821 MS.gene004543:CDS 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.1:56938558-56938577 None:intergenic 25.0%
! GGTAATAATGTTGAAAAAGA+GGG - chr2.1:56938266-56938285 None:intergenic 25.0%
! GTTTCTTGATAAGTGTATAA+AGG + chr2.1:56939265-56939284 MS.gene004543:CDS 25.0%
! TATAAATCAATTGATAGGCT+TGG - chr2.1:56938665-56938684 None:intergenic 25.0%
! TTAAAAGAATGCTTTGGTTA+AGG + chr2.1:56938808-56938827 MS.gene004543:CDS 25.0%
!! ATCAACTTCTTTAGAAACTA+TGG + chr2.1:56938453-56938472 MS.gene004543:CDS 25.0%
!! CATTCTTTTAATGTTTGAGT+CGG - chr2.1:56938799-56938818 None:intergenic 25.0%
!! TTCTTTAAGTTTCGAAAAGA+GGG + chr2.1:56939145-56939164 MS.gene004543:CDS 25.0%
!! TTTCTTTAAGTTTCGAAAAG+AGG + chr2.1:56939144-56939163 MS.gene004543:CDS 25.0%
!!! TAAGTGTATAAAGGGTATTA+AGG + chr2.1:56939274-56939293 MS.gene004543:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.1:56938561-56938580 MS.gene004543:CDS 25.0%
AAAAGCCAAAATTGTTGATG+AGG - chr2.1:56938359-56938378 None:intergenic 30.0%
AATGTTGAAAAAGAGGGTTA+GGG - chr2.1:56938260-56938279 None:intergenic 30.0%
ATCTGTAAGGAAAACATTGT+GGG + chr2.1:56938944-56938963 MS.gene004543:CDS 30.0%
ATGTTCTACAAGCATTTAGA+AGG + chr2.1:56939012-56939031 MS.gene004543:CDS 30.0%
CTTAAAACTAATTGCGTATG+TGG - chr2.1:56938409-56938428 None:intergenic 30.0%
GAAAAATGGAGAGAAAATGT+TGG - chr2.1:56938297-56938316 None:intergenic 30.0%
GGGTAATAATGTTGAAAAAG+AGG - chr2.1:56938267-56938286 None:intergenic 30.0%
TAATGTTGAAAAAGAGGGTT+AGG - chr2.1:56938261-56938280 None:intergenic 30.0%
TATCTGTAAGGAAAACATTG+TGG + chr2.1:56938943-56938962 MS.gene004543:CDS 30.0%
! TGTTTTCCTTACAGATAGTT+TGG - chr2.1:56938940-56938959 None:intergenic 30.0%
! TTATCAAGAAACAGCTTTTC+AGG - chr2.1:56939257-56939276 None:intergenic 30.0%
! TTGCAATATCTTCTGAAGAA+AGG + chr2.1:56939188-56939207 MS.gene004543:CDS 30.0%
!! TATTATTGAGGCTTGTTTTC+AGG - chr2.1:56938635-56938654 None:intergenic 30.0%
AATTTCAGCATGTCATGTGT+CGG - chr2.1:56938852-56938871 None:intergenic 35.0%
ACTACGTCTTACCATTTCAA+AGG + chr2.1:56938491-56938510 MS.gene004543:CDS 35.0%
AGAAAAAACTGATGGTCCTT+TGG - chr2.1:56939129-56939148 None:intergenic 35.0%
ATGGATACCAACATACAATG+TGG - chr2.1:56939041-56939060 None:intergenic 35.0%
ATTCGCAATCCATCTTTCTT+CGG + chr2.1:56938723-56938742 MS.gene004543:CDS 35.0%
CAGAAGATATTGCAATACAG+AGG - chr2.1:56939183-56939202 None:intergenic 35.0%
GAAAATGCTAACAATGTCAG+AGG - chr2.1:56938595-56938614 None:intergenic 35.0%
GAAATTGGTGGAGGAATTAA+AGG + chr2.1:56938866-56938885 MS.gene004543:CDS 35.0%
GAAGAAAGGTTTGCGAAAAA+AGG + chr2.1:56939202-56939221 MS.gene004543:CDS 35.0%
GCTACTTCAAAAACAACCTT+TGG + chr2.1:56938900-56938919 MS.gene004543:CDS 35.0%
GTGGAGGAATTAAAGGATTT+GGG + chr2.1:56938873-56938892 MS.gene004543:CDS 35.0%
TAGATTCATGCAATCAGACA+AGG + chr2.1:56938683-56938702 MS.gene004543:CDS 35.0%
TGAAATGGTAAGACGTAGTT+GGG - chr2.1:56938490-56938509 None:intergenic 35.0%
TTACCATTTCAAAGGAACCA+TGG + chr2.1:56938499-56938518 MS.gene004543:CDS 35.0%
TTGAAATGGTAAGACGTAGT+TGG - chr2.1:56938491-56938510 None:intergenic 35.0%
! ATTAGATTGCTGATTGCCAA+TGG + chr2.1:56938768-56938787 MS.gene004543:CDS 35.0%
! GATAACAGAGTCAGGTTTTT+GGG - chr2.1:56938436-56938455 None:intergenic 35.0%
! TCCTACCTCATCAACAATTT+TGG + chr2.1:56938351-56938370 MS.gene004543:CDS 35.0%
! TGATAACAGAGTCAGGTTTT+TGG - chr2.1:56938437-56938456 None:intergenic 35.0%
! TTTGGCAAAACCCTTTTAGA+AGG - chr2.1:56938540-56938559 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+AGG + chr2.1:56939083-56939102 MS.gene004543:CDS 35.0%
!! AAGAAGTTGATAACAGAGTC+AGG - chr2.1:56938444-56938463 None:intergenic 35.0%
!! AGAGAAAATGTTGGTGATGA+TGG - chr2.1:56938288-56938307 None:intergenic 35.0%
!! GAGAAAATGTTGGTGATGAT+GGG - chr2.1:56938287-56938306 None:intergenic 35.0%
!! TTGGCAAAACCCTTTTAGAA+GGG - chr2.1:56938539-56938558 None:intergenic 35.0%
AAAGTCTGGAAGCAGATTGT+GGG - chr2.1:56938383-56938402 None:intergenic 40.0%
AAGGCAACCACATTGTATGT+TGG + chr2.1:56939031-56939050 MS.gene004543:CDS 40.0%
AATGCTTTGGTTAAGGAGTC+GGG + chr2.1:56938815-56938834 MS.gene004543:CDS 40.0%
ACATGACATGCTGAAATTGG+TGG + chr2.1:56938854-56938873 MS.gene004543:CDS 40.0%
ACTCAATCTTGCTTACACCA+TGG + chr2.1:56939331-56939350 MS.gene004543:CDS 40.0%
AGAGAAAAGCTCTATGACGA+TGG - chr2.1:56938203-56938222 None:intergenic 40.0%
AGAGGTTGAATCTGAAGAGT+TGG - chr2.1:56938322-56938341 None:intergenic 40.0%
GAAAAAGAGGGTTAGGGTTA+GGG - chr2.1:56938254-56938273 None:intergenic 40.0%
GAATGCTTTGGTTAAGGAGT+CGG + chr2.1:56938814-56938833 MS.gene004543:CDS 40.0%
GACACATGACATGCTGAAAT+TGG + chr2.1:56938851-56938870 MS.gene004543:CDS 40.0%
GGTGGAGGAATTAAAGGATT+TGG + chr2.1:56938872-56938891 MS.gene004543:CDS 40.0%
TGAAAAAGAGGGTTAGGGTT+AGG - chr2.1:56938255-56938274 None:intergenic 40.0%
TGCATGCCAAACTATCTGTA+AGG + chr2.1:56938931-56938950 MS.gene004543:CDS 40.0%
TTGGGTCAATCAGTTAGGTT+GGG + chr2.1:56939088-56939107 MS.gene004543:CDS 40.0%
TTTCCTGCAACCCTTCTAAA+AGG + chr2.1:56938526-56938545 MS.gene004543:CDS 40.0%
TTTGGGTCAATCAGTTAGGT+TGG + chr2.1:56939087-56939106 MS.gene004543:CDS 40.0%
! AAGTCCATGCCTTTAAGAAG+TGG + chr2.1:56938973-56938992 MS.gene004543:CDS 40.0%
! AGTCCATGCCTTTAAGAAGT+GGG + chr2.1:56938974-56938993 MS.gene004543:CDS 40.0%
! ATTGTTGATGAGGTAGGAGA+CGG - chr2.1:56938349-56938368 None:intergenic 40.0%
! GCCAAAATTGTTGATGAGGT+AGG - chr2.1:56938355-56938374 None:intergenic 40.0%
! TAGCCATGGTTCCTTTGAAA+TGG - chr2.1:56938505-56938524 None:intergenic 40.0%
! TTCCTGCAACCCTTCTAAAA+GGG + chr2.1:56938527-56938546 MS.gene004543:CDS 40.0%
!! AGCTTTTCAGGCACTACAAA+TGG - chr2.1:56939245-56939264 None:intergenic 40.0%
!! AGGCTTGTTTTCAGGATGTA+GGG - chr2.1:56938627-56938646 None:intergenic 40.0%
!! AGGGTTTGGTGAGAAAGTTA+AGG - chr2.1:56938235-56938254 None:intergenic 40.0%
!! CTGAAGAGTTGGTGGAAAAA+TGG - chr2.1:56938311-56938330 None:intergenic 40.0%
AAACTGATGGTCCTTTGGCA+AGG - chr2.1:56939124-56939143 None:intergenic 45.0%
AACCCCACTTCTTAAAGGCA+TGG - chr2.1:56938980-56938999 None:intergenic 45.0%
AACTGATGGTCCTTTGGCAA+GGG - chr2.1:56939123-56939142 None:intergenic 45.0%
AAGTCTGGAAGCAGATTGTG+GGG - chr2.1:56938382-56938401 None:intergenic 45.0%
AGACCAACCCCACTTCTTAA+AGG - chr2.1:56938985-56939004 None:intergenic 45.0%
CAAAGTCTGGAAGCAGATTG+TGG - chr2.1:56938384-56938403 None:intergenic 45.0%
GGTTGAATCTGAAGAGTTGG+TGG - chr2.1:56938319-56938338 None:intergenic 45.0%
TATTGCAATACAGAGGCCCT+TGG - chr2.1:56939176-56939195 None:intergenic 45.0%
TGACATGCTGAAATTGGTGG+AGG + chr2.1:56938857-56938876 MS.gene004543:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG + chr2.1:56938979-56938998 MS.gene004543:CDS 45.0%
! ATTGCTGATTGCCAATGGAG+TGG + chr2.1:56938773-56938792 MS.gene004543:CDS 45.0%
! CGATGTGCATGTTTTGTCCA+TGG - chr2.1:56939351-56939370 None:intergenic 45.0%
! GTCCATGCCTTTAAGAAGTG+GGG + chr2.1:56938975-56938994 MS.gene004543:CDS 45.0%
!! AACCCTTTTAGAAGGGTTGC+AGG - chr2.1:56938532-56938551 None:intergenic 45.0%
!! GAGGCTTGTTTTCAGGATGT+AGG - chr2.1:56938628-56938647 None:intergenic 45.0%
!! GGCTTGTTTTCAGGATGTAG+GGG - chr2.1:56938626-56938645 None:intergenic 45.0%
AGAGGGTTAGGGTTAGGGTT+TGG - chr2.1:56938249-56938268 None:intergenic 50.0%
CGAAAAGAGGGTCCTTCCAA+GGG + chr2.1:56939157-56939176 MS.gene004543:CDS 50.0%
GGCATGCAATGCTACACCAA+AGG - chr2.1:56938919-56938938 None:intergenic 50.0%
TCGAAAAGAGGGTCCTTCCA+AGG + chr2.1:56939156-56939175 MS.gene004543:CDS 50.0%
TGCGTATGTGGAGCAAAGTC+TGG - chr2.1:56938397-56938416 None:intergenic 50.0%
CGAGCGTCTCCGAAGAAAGA+TGG - chr2.1:56938735-56938754 None:intergenic 55.0%
GAGGTAGGAGACGGCAAAAG+AGG - chr2.1:56938340-56938359 None:intergenic 55.0%
GATGCACTTGCCCTTGCCAA+AGG + chr2.1:56939110-56939129 MS.gene004543:CDS 55.0%
GCAATACAGAGGCCCTTGGA+AGG - chr2.1:56939172-56939191 None:intergenic 55.0%
GGGTTGCAGGAAAGTAGCCA+TGG - chr2.1:56938519-56938538 None:intergenic 55.0%
GTTTGAGTCGGCCACTCCAT+TGG - chr2.1:56938787-56938806 None:intergenic 55.0%
TTTCTTCGGAGACGCTCGTG+TGG + chr2.1:56938737-56938756 MS.gene004543:CDS 55.0%
!! GTCAGAGGTAGAAGCGCCTT+TGG - chr2.1:56938580-56938599 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56938189 56939373 56938189 ID=MS.gene004543
chr2.1 mRNA 56938189 56939373 56938189 ID=MS.gene004543.t1;Parent=MS.gene004543
chr2.1 exon 56938189 56939373 56938189 ID=MS.gene004543.t1.exon1;Parent=MS.gene004543.t1
chr2.1 CDS 56938189 56939373 56938189 ID=cds.MS.gene004543.t1;Parent=MS.gene004543.t1
Gene Sequence

>MS.gene004543

ATGTTGAATATCCATCGTCATAGAGCTTTTCTCTATCTCAAAGCCTTAACTTTCTCACCAAACCCTAACCCTAACCCTCTTTTTCAACATTATTACCCATCATCACCAACATTTTCTCTCCATTTTTCCACCAACTCTTCAGATTCAACCTCTTTTGCCGTCTCCTACCTCATCAACAATTTTGGCTTTTCCCCACAATCTGCTTCCAGACTTTGCTCCACATACGCAATTAGTTTTAAGACTGCCCAAAAACCTGACTCTGTTATCAACTTCTTTAGAAACTATGGTTTTTCAGATTCCCAACTACGTCTTACCATTTCAAAGGAACCATGGCTACTTTCCTGCAACCCTTCTAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACCTCTGACATTGTTAGCATTTTCACTAAGAGCCCCTACATCCTGAAAACAAGCCTCAATAATAAAATTATCCAAGCCTATCAATTGATTTATAGATTCATGCAATCAGACAAGGACATTATTGATTGTGTAATTCGCAATCCATCTTTCTTCGGAGACGCTCGTGTGGTATCTAACATTAGATTGCTGATTGCCAATGGAGTGGCCGACTCAAACATTAAAAGAATGCTTTGGTTAAGGAGTCGGGCATTTCAGACACCGACACATGACATGCTGAAATTGGTGGAGGAATTAAAGGATTTGGGTTTTGCTACTTCAAAAACAACCTTTGGTGTAGCATTGCATGCCAAACTATCTGTAAGGAAAACATTGTGGGAAGAGAAAGTCCATGCCTTTAAGAAGTGGGGTTGGTCTGATGAAGATGTTCTACAAGCATTTAGAAGGCAACCACATTGTATGTTGGTATCCATTGATAAAATTAATTTAGTGATGAACTTTTGGGTCAATCAGTTAGGTTGGGATGCACTTGCCCTTGCCAAAGGACCATCAGTTTTTTCTTTAAGTTTCGAAAAGAGGGTCCTTCCAAGGGCCTCTGTATTGCAATATCTTCTGAAGAAAGGTTTGCGAAAAAAGGATGCAAGCTTAACTTGTCCATTTGTAGTGCCTGAAAAGCTGTTTCTTGATAAGTGTATAAAGGGTATTAAGGAGTCTTCTTATCTATTAAAGTTGTATGAGGAAAAACTCAATCTTGCTTACACCATGGACAAAACATGCACATCGTGA
Protein sequence

>MS.gene004543.t1

MLNIHRHRAFLYLKALTFSPNPNPNPLFQHYYPSSPTFSLHFSTNSSDSTSFAVSYLINNFGFSPQSASRLCSTYAISFKTAQKPDSVINFFRNYGFSDSQLRLTISKEPWLLSCNPSKRVLPKFQFFLSKGASTSDIVSIFTKSPYILKTSLNNKIIQAYQLIYRFMQSDKDIIDCVIRNPSFFGDARVVSNIRLLIANGVADSNIKRMLWLRSRAFQTPTHDMLKLVEELKDLGFATSKTTFGVALHAKLSVRKTLWEEKVHAFKKWGWSDEDVLQAFRRQPHCMLVSIDKINLVMNFWVNQLGWDALALAKGPSVFSLSFEKRVLPRASVLQYLLKKGLRKKDASLTCPFVVPEKLFLDKCIKGIKESSYLLKLYEEKLNLAYTMDKTCTS