AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004587

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004587.t1 MTR_2g437150 75.862 406 92 2 1 400 1 406 0.0 626
MS.gene004587.t1 MTR_2g437170 76.427 403 89 2 1 397 1 403 0.0 623
MS.gene004587.t1 MTR_2g437120 74.874 398 99 1 1 398 1 397 0.0 598
MS.gene004587.t1 MTR_2g437180 73.000 400 104 2 1 400 1 396 0.0 587
MS.gene004587.t1 MTR_2g437100 75.635 394 81 3 1 384 7 395 0.0 577
MS.gene004587.t1 MTR_2g437160 70.545 404 111 3 1 400 12 411 0.0 569
MS.gene004587.t1 MTR_2g437960 70.574 401 112 2 1 397 1 399 0.0 551
MS.gene004587.t1 MTR_2g437960 70.574 401 112 2 1 397 1 399 0.0 551
MS.gene004587.t1 MTR_2g437990 68.486 403 117 4 1 399 1 397 0.0 532
MS.gene004587.t1 MTR_2g438010 66.998 403 124 3 1 399 21 418 0.0 528
MS.gene004587.t1 MTR_2g076320 67.742 403 121 3 1 399 1 398 0.0 523
MS.gene004587.t1 MTR_2g437940 66.416 399 104 2 1 399 1 369 0.0 516
MS.gene004587.t1 MTR_2g437240 68.687 396 85 3 36 396 19 410 0.0 512
MS.gene004587.t1 MTR_2g437200 62.148 391 105 3 35 384 10 398 3.57e-158 451
MS.gene004587.t1 MTR_2g438020 61.299 385 111 3 50 399 70 451 6.44e-156 449
MS.gene004587.t1 MTR_2g437130 60.000 380 140 4 20 399 19 386 5.63e-151 432
MS.gene004587.t1 MTR_2g437080 52.825 354 164 1 43 396 32 382 7.24e-130 379
MS.gene004587.t1 MTR_2g436440 51.064 376 180 2 21 396 17 388 5.04e-128 374
MS.gene004587.t1 MTR_2g437020 55.114 352 152 3 43 392 31 378 2.62e-127 372
MS.gene004587.t1 MTR_2g437060 52.078 361 169 2 32 392 37 393 6.74e-127 371
MS.gene004587.t1 MTR_2g436400 48.794 373 175 4 16 388 25 381 6.75e-123 361
MS.gene004587.t1 MTR_2g437030 51.714 350 166 1 43 392 118 464 1.41e-121 360
MS.gene004587.t1 MTR_2g437040 49.333 375 179 2 22 396 19 382 3.07e-119 352
MS.gene004587.t1 MTR_2g437260 50.992 353 165 5 39 388 23 370 2.23e-116 345
MS.gene004587.t1 MTR_4g119550 47.701 348 176 3 46 392 46 388 4.34e-113 336
MS.gene004587.t1 MTR_4g119580 49.555 337 165 2 46 382 39 370 5.70e-103 310
MS.gene004587.t1 MTR_4g120380 48.225 338 170 2 45 382 38 370 2.24e-102 308
MS.gene004587.t1 MTR_2g436460 47.661 342 173 5 45 385 41 377 1.46e-99 301
MS.gene004587.t1 MTR_4g120380 47.761 335 161 3 45 379 38 358 1.21e-97 298
MS.gene004587.t1 MTR_2g437160 70.588 187 55 0 214 400 134 320 2.40e-91 278
MS.gene004587.t1 MTR_2g437160 64.430 149 42 3 1 145 12 153 2.81e-50 172
MS.gene004587.t1 MTR_2g019840 41.429 350 194 7 45 391 37 378 2.74e-83 259
MS.gene004587.t1 MTR_2g019810 43.695 341 173 7 51 384 31 359 1.36e-82 257
MS.gene004587.t1 MTR_2g437090 53.801 171 79 0 227 397 1 171 6.81e-62 197
MS.gene004587.t1 MTR_3g450500 31.548 336 223 4 53 384 40 372 4.58e-53 181
MS.gene004587.t1 MTR_4g049400 60.256 156 53 2 1 152 1 151 2.28e-52 175
MS.gene004587.t1 MTR_4g119570 38.889 252 118 2 131 382 14 229 3.52e-49 166
MS.gene004587.t1 MTR_4g007490 30.618 356 241 4 30 382 16 368 1.15e-47 167
MS.gene004587.t1 MTR_2g436370 47.619 168 73 2 43 210 38 190 3.35e-44 152
MS.gene004587.t1 MTR_8g081000 29.605 304 210 2 81 382 84 385 2.57e-35 134
MS.gene004587.t1 MTR_8g081000 29.605 304 210 2 81 382 84 385 3.23e-35 134
MS.gene004587.t1 MTR_8g081000 29.605 304 210 2 81 382 84 385 5.18e-35 134
MS.gene004587.t1 MTR_8g080990 29.866 298 205 2 83 378 73 368 3.09e-30 120
MS.gene004587.t1 MTR_3g085240 62.637 91 17 1 284 374 174 247 1.57e-26 107
MS.gene004587.t1 MTR_3g085240 40.000 190 91 7 36 223 44 212 2.85e-23 98.2
MS.gene004587.t1 MTR_2g436380 42.424 99 54 1 227 325 1 96 4.62e-16 78.6
MS.gene004587.t1 MTR_8g012210 21.166 326 247 6 81 398 47 370 1.02e-11 66.2
MS.gene004587.t1 MTR_4g119615 55.556 54 23 1 321 374 22 74 1.07e-11 61.2
MS.gene004587.t1 MTR_2g034600 24.803 254 170 6 90 332 215 458 1.71e-11 66.2
MS.gene004587.t1 MTR_2g034600 24.803 254 170 6 90 332 280 523 1.92e-11 66.2
MS.gene004587.t1 MTR_2g034600 24.803 254 170 6 90 332 276 519 1.93e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004587.t1 AT5G07900 32.537 335 215 4 53 381 70 399 3.32e-57 192
MS.gene004587.t1 AT1G21150 33.030 330 216 3 53 379 57 384 7.86e-52 178
MS.gene004587.t1 AT1G21150 32.335 334 221 3 49 379 17 348 2.49e-51 176
MS.gene004587.t1 AT1G21150 33.030 330 216 3 53 379 121 448 3.15e-51 178
MS.gene004587.t1 AT1G61990 30.056 356 196 8 53 368 53 395 3.79e-37 139
MS.gene004587.t1 AT1G61990 30.056 356 196 8 53 368 53 395 3.79e-37 139
MS.gene004587.t1 AT1G61980 28.531 354 207 8 53 367 53 399 8.72e-37 139
MS.gene004587.t1 AT1G61980 28.531 354 207 8 53 367 53 399 8.72e-37 139
MS.gene004587.t1 AT1G61980 28.531 354 207 8 53 367 53 399 8.72e-37 139
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G61970 28.451 355 206 9 53 367 53 399 8.15e-36 136
MS.gene004587.t1 AT1G62120 28.249 354 214 7 51 367 55 405 3.05e-33 129
MS.gene004587.t1 AT5G64950 26.611 357 232 7 32 373 25 366 5.49e-29 117
MS.gene004587.t1 AT3G46950 29.889 271 175 6 35 299 30 291 1.27e-26 111
MS.gene004587.t1 AT1G62085 27.855 359 239 10 53 400 56 405 9.21e-24 103
MS.gene004587.t1 AT1G62085 27.855 359 239 10 53 400 56 405 9.21e-24 103
MS.gene004587.t1 AT1G62010 27.119 295 172 5 87 344 71 359 2.04e-23 101
MS.gene004587.t1 AT1G62150 26.870 361 240 8 53 400 59 408 4.60e-23 100
MS.gene004587.t1 AT1G61960 28.309 272 190 3 26 296 26 293 6.39e-23 100
MS.gene004587.t1 AT1G62110 29.032 248 166 4 53 296 53 294 4.84e-22 98.2
MS.gene004587.t1 AT5G23930 27.881 269 175 5 27 286 22 280 1.36e-18 87.8

Find 72 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCCTTCATTGGCTACTTT+TGG 0.140203 2.2:-52132218 MS.gene004587:CDS
GTCAATTTGATGTCTGATTT+TGG 0.159011 2.2:-52132344 MS.gene004587:CDS
TCATACCTCATCAACAATTT+TGG 0.165317 2.2:-52132770 MS.gene004587:CDS
AAGCTAATGCGTAAAATTCT+AGG 0.232966 2.2:+52132497 None:intergenic
ATAACTGAATCAGGCTTATC+TGG 0.241119 2.2:+52132689 None:intergenic
AAAAGAGGGTTGTTGAGTTT+TGG 0.250282 2.2:+52132869 None:intergenic
TTGGAGGAAGTTAAGTGTTT+AGG 0.257551 2.2:-52132245 MS.gene004587:CDS
AAGTCAAGGCCATCTATATT+TGG 0.261665 2.2:-52132290 MS.gene004587:CDS
TGGAGGAAGTTAAGTGTTTA+GGG 0.262883 2.2:-52132244 MS.gene004587:CDS
AAACTACCAATGACATTATT+AGG 0.279319 2.2:+52132317 None:intergenic
TGGCATACCATTCACTAATT+AGG 0.304191 2.2:-52131754 None:intergenic
ATGCCATTCTTTGTTTAATT+CGG 0.306732 2.2:-52132415 MS.gene004587:CDS
AGACCAGCCCCATTTCTTAA+AGG 0.307294 2.2:+52132136 None:intergenic
TGATTCCTAAAACAACATTC+TGG 0.312730 2.2:+52132912 None:intergenic
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2.2:-52132264 MS.gene004587:CDS
ATTGAAACCAACATCAAATT+AGG 0.361121 2.2:+52132080 None:intergenic
AAGTTGATGCCTTTAAGAAA+TGG 0.364427 2.2:-52132145 MS.gene004587:CDS
TTTGATGATGATGCGTATAT+CGG 0.373047 2.2:+52132634 None:intergenic
AGAAGCTTCTGAGTGAAATT+AGG 0.377101 2.2:+52132828 None:intergenic
AAGAAGGTGATAACTGAATC+AGG 0.385522 2.2:+52132680 None:intergenic
AGTTGATGCCTTTAAGAAAT+GGG 0.392127 2.2:-52132144 MS.gene004587:CDS
TCCTTTGAACCAAATATAGA+TGG 0.398078 2.2:+52132281 None:intergenic
ACAAAGAATGGCATCCTTGT+TGG 0.401490 2.2:+52132424 None:intergenic
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2.2:+52132544 None:intergenic
TTCAGCTTTAAGAAGGAGTC+TGG 0.405388 2.2:-52131834 MS.gene004587:CDS
GGAGTTCCTAATAATGTCAT+TGG 0.406851 2.2:-52132323 MS.gene004587:CDS
TTTGTTATAGTTTGGAGAAA+AGG 0.408694 2.2:-52131974 MS.gene004587:CDS
TTTAGGGTTTCATCCTTCAT+TGG 0.414145 2.2:-52132228 MS.gene004587:CDS
GCCATCTATATTTGGTTCAA+AGG 0.427799 2.2:-52132282 MS.gene004587:CDS
AGAAAATGGTGCAGCTAAGC+TGG 0.430292 2.2:+52131890 None:intergenic
CTTTGGCAAAATAACATTGT+GGG 0.433749 2.2:+52132583 None:intergenic
TCAATTGGGTTGGAATTCCT+TGG 0.440659 2.2:-52132021 MS.gene004587:CDS
GAGAAGTGTTGGTTCAAAAG+AGG 0.453001 2.2:+52132854 None:intergenic
GAAATTACCTAATTAGTGAA+TGG 0.466047 2.2:+52131747 None:intergenic
GATTCCTAAAACAACATTCT+GGG 0.469695 2.2:+52132913 None:intergenic
AAACTATAACAAAACATTTG+TGG 0.471875 2.2:+52131984 None:intergenic
ATTATCCACAGTAAGGTGAT+AGG 0.476240 2.2:+52132367 None:intergenic
ATGCCGAATTAAACAAAGAA+TGG 0.493192 2.2:+52132412 None:intergenic
AGCTAATGCGTAAAATTCTA+GGG 0.502169 2.2:+52132498 None:intergenic
ATGAGGTATGATACAGCAAA+TGG 0.505053 2.2:+52132782 None:intergenic
TTTGGGTCAATCAATTGGGT+TGG 0.506924 2.2:-52132031 MS.gene004587:CDS
GTTGCAGCCTAATTTGATGT+TGG 0.507199 2.2:-52132087 MS.gene004587:CDS
AAATTAGGAACGAGAAGTGT+TGG 0.518663 2.2:+52132843 None:intergenic
TAACTGAATCAGGCTTATCT+GGG 0.520618 2.2:+52132690 None:intergenic
CAAAGCTTTCAACCTCAAGC+AGG 0.538715 2.2:-52132726 MS.gene004587:CDS
ACCCCAAAAGTAGCCAATGA+AGG 0.540625 2.2:+52132215 None:intergenic
AATTGCATAACTGAGGCCCT+TGG 0.542717 2.2:+52131945 None:intergenic
AGAAGTGTTGGTTCAAAAGA+GGG 0.551032 2.2:+52132855 None:intergenic
ATGCCTTTAAGAAATGGGGC+TGG 0.559676 2.2:-52132139 MS.gene004587:CDS
AATTGACATTATCCACAGTA+AGG 0.560808 2.2:+52132360 None:intergenic
TGGTAGTTTGATAAGTGCCA+AGG 0.560890 2.2:+52132004 None:intergenic
TTGGTAGTTTGCTTAAGTCA+AGG 0.561949 2.2:-52132304 MS.gene004587:CDS
ACTTTGGCAAAATAACATTG+TGG 0.562030 2.2:+52132582 None:intergenic
TGGAGAAAAGGATCATTCCA+AGG 0.570897 2.2:-52131962 MS.gene004587:CDS
GGATTTGATTAAGTCATTGG+AGG 0.571043 2.2:-52132261 MS.gene004587:CDS
TTTGGCAAAATAACATTGTG+GGG 0.586854 2.2:+52132584 None:intergenic
AAGAAACAGTTTCTTCAACA+TGG 0.593202 2.2:+52132937 None:intergenic
TGAAACCAAAATTGTTGATG+AGG 0.604511 2.2:+52132765 None:intergenic
TATCGGAGTGTGAAAAGCCA+TGG 0.608796 2.2:+52132651 None:intergenic
AATGAAACTTGCATACACAA+TGG 0.609213 2.2:-52131784 MS.gene004587:CDS
TATTGAAACGAACCTGCTTG+AGG 0.609752 2.2:+52132714 None:intergenic
ACTCAGAAGCTTCTGCACAT+CGG 0.615740 2.2:-52132819 MS.gene004587:CDS
GTTGATGCCTTTAAGAAATG+GGG 0.618010 2.2:-52132143 MS.gene004587:CDS
CAAAAGAAATTGCATAACTG+AGG 0.634260 2.2:+52131938 None:intergenic
TGAGGTATGATACAGCAAAT+GGG 0.638616 2.2:+52132783 None:intergenic
GGAGAAAAGGATCATTCCAA+GGG 0.639024 2.2:-52131961 MS.gene004587:CDS
GCATACACAATGGAAAACAA+TGG 0.644592 2.2:-52131774 MS.gene004587:CDS
AACTGAATCAGGCTTATCTG+GGG 0.674780 2.2:+52132691 None:intergenic
GAATTCCTATCACCTTACTG+TGG 0.683150 2.2:-52132372 MS.gene004587:CDS
GGTTGTGATGAAAGTAACGA+AGG 0.709568 2.2:+52132605 None:intergenic
AAAGCTTTGAGAGCTGACTG+AGG 0.710331 2.2:+52132740 None:intergenic
TAGGTTTATGAAAACCAACA+AGG 0.716946 2.2:-52132438 MS.gene004587:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTATAACAAAACATTTG+TGG + chr2.2:52132709-52132728 None:intergenic 20.0%
!! AATGTTTGAGTAAAAAACTT+TGG - chr2.2:52132515-52132534 MS.gene004587:CDS 20.0%
!! ATGTTTGAGTAAAAAACTTT+GGG - chr2.2:52132516-52132535 MS.gene004587:CDS 20.0%
!! GATAGAAAAAATTGAAACTT+TGG + chr2.2:52132127-52132146 None:intergenic 20.0%
!! TACTTAACAATTCAAAAAGA+GGG + chr2.2:52132234-52132253 None:intergenic 20.0%
!!! ACAAATGTTTTGTTATAGTT+TGG - chr2.2:52132708-52132727 MS.gene004587:CDS 20.0%
!!! ATTTTCTCAGAATAAGAAAA+TGG + chr2.2:52132817-52132836 None:intergenic 20.0%
!!! TTTCAATTTTTTCTATCCAA+AGG - chr2.2:52132130-52132149 MS.gene004587:CDS 20.0%
!!! TTTTTTACTCAAACATTTCT+TGG + chr2.2:52132512-52132531 None:intergenic 20.0%
! AAACTACCAATGACATTATT+AGG + chr2.2:52132376-52132395 None:intergenic 25.0%
! AAAGGATTTGATTAAGTCAT+TGG - chr2.2:52132426-52132445 MS.gene004587:CDS 25.0%
! ATTGAAACCAACATCAAATT+AGG + chr2.2:52132613-52132632 None:intergenic 25.0%
! CTACTTAACAATTCAAAAAG+AGG + chr2.2:52132235-52132254 None:intergenic 25.0%
!! ATGCAATTTCTTTTGATGAA+AGG - chr2.2:52132760-52132779 MS.gene004587:CDS 25.0%
!! ATGCCATTCTTTGTTTAATT+CGG - chr2.2:52132275-52132294 MS.gene004587:CDS 25.0%
!! CTCTTTTTGAATTGTTAAGT+AGG - chr2.2:52132233-52132252 MS.gene004587:CDS 25.0%
!! TTTGTTATAGTTTGGAGAAA+AGG - chr2.2:52132716-52132735 MS.gene004587:CDS 25.0%
!!! TGATTTGTTGATGAGTTTTT+GGG - chr2.2:52132642-52132661 MS.gene004587:CDS 25.0%
!!! TTGATTTGTTGATGAGTTTT+TGG - chr2.2:52132641-52132660 MS.gene004587:CDS 25.0%
AAGCTAATGCGTAAAATTCT+AGG + chr2.2:52132196-52132215 None:intergenic 30.0%
AATGAAACTTGCATACACAA+TGG - chr2.2:52132906-52132925 MS.gene004587:CDS 30.0%
AATTGACATTATCCACAGTA+AGG + chr2.2:52132333-52132352 None:intergenic 30.0%
ACTTTGGCAAAATAACATTG+TGG + chr2.2:52132111-52132130 None:intergenic 30.0%
AGCTAATGCGTAAAATTCTA+GGG + chr2.2:52132195-52132214 None:intergenic 30.0%
ATGCCGAATTAAACAAAGAA+TGG + chr2.2:52132281-52132300 None:intergenic 30.0%
CAAAAGAAATTGCATAACTG+AGG + chr2.2:52132755-52132774 None:intergenic 30.0%
CTTTGGCAAAATAACATTGT+GGG + chr2.2:52132110-52132129 None:intergenic 30.0%
GATTCCTAAAACAACATTCT+GGG + chr2.2:52131780-52131799 None:intergenic 30.0%
TAGGTTTATGAAAACCAACA+AGG - chr2.2:52132252-52132271 MS.gene004587:CDS 30.0%
TCCTTTGAACCAAATATAGA+TGG + chr2.2:52132412-52132431 None:intergenic 30.0%
TGAAACCAAAATTGTTGATG+AGG + chr2.2:52131928-52131947 None:intergenic 30.0%
TGATTCCTAAAACAACATTC+TGG + chr2.2:52131781-52131800 None:intergenic 30.0%
TTTGGCAAAATAACATTGTG+GGG + chr2.2:52132109-52132128 None:intergenic 30.0%
! AGTTGATGCCTTTAAGAAAT+GGG - chr2.2:52132546-52132565 MS.gene004587:CDS 30.0%
! GTCAATTTGATGTCTGATTT+TGG - chr2.2:52132346-52132365 MS.gene004587:CDS 30.0%
! TAGAATTTTACGCATTAGCT+TGG - chr2.2:52132195-52132214 MS.gene004587:CDS 30.0%
! TCATACCTCATCAACAATTT+TGG - chr2.2:52131920-52131939 MS.gene004587:CDS 30.0%
!! AAGTTGATGCCTTTAAGAAA+TGG - chr2.2:52132545-52132564 MS.gene004587:CDS 30.0%
!! TTTGATGATGATGCGTATAT+CGG + chr2.2:52132059-52132078 None:intergenic 30.0%
!!! AGTTTTTGGGTCAATCAATT+GGG - chr2.2:52132655-52132674 MS.gene004587:CDS 30.0%
!!! GTTTGTTTTCAGCTTTAAGA+AGG - chr2.2:52132849-52132868 MS.gene004587:CDS 30.0%
AAGAAGGTGATAACTGAATC+AGG + chr2.2:52132013-52132032 None:intergenic 35.0%
AAGTCAAGGCCATCTATATT+TGG - chr2.2:52132400-52132419 MS.gene004587:CDS 35.0%
AGAAGCTTCTGAGTGAAATT+AGG + chr2.2:52131865-52131884 None:intergenic 35.0%
ATAACTGAATCAGGCTTATC+TGG + chr2.2:52132004-52132023 None:intergenic 35.0%
ATCACCTTCTTCAAAAACCA+TGG - chr2.2:52132022-52132041 MS.gene004587:CDS 35.0%
ATGAGGTATGATACAGCAAA+TGG + chr2.2:52131911-52131930 None:intergenic 35.0%
ATTATCCACAGTAAGGTGAT+AGG + chr2.2:52132326-52132345 None:intergenic 35.0%
GCATACACAATGGAAAACAA+TGG - chr2.2:52132916-52132935 MS.gene004587:CDS 35.0%
GGAGTTCCTAATAATGTCAT+TGG - chr2.2:52132367-52132386 MS.gene004587:CDS 35.0%
GGATTTGATTAAGTCATTGG+AGG - chr2.2:52132429-52132448 MS.gene004587:CDS 35.0%
TAACTGAATCAGGCTTATCT+GGG + chr2.2:52132003-52132022 None:intergenic 35.0%
TGAGGTATGATACAGCAAAT+GGG + chr2.2:52131910-52131929 None:intergenic 35.0%
TGGAGGAAGTTAAGTGTTTA+GGG - chr2.2:52132446-52132465 MS.gene004587:CDS 35.0%
TTGGAGGAAGTTAAGTGTTT+AGG - chr2.2:52132445-52132464 MS.gene004587:CDS 35.0%
! AAAAGAGGGTTGTTGAGTTT+TGG + chr2.2:52131824-52131843 None:intergenic 35.0%
! AAAGAGGGTTGTTGAGTTTT+GGG + chr2.2:52131823-52131842 None:intergenic 35.0%
! AAATTAGGAACGAGAAGTGT+TGG + chr2.2:52131850-52131869 None:intergenic 35.0%
! GCCATCTATATTTGGTTCAA+AGG - chr2.2:52132408-52132427 MS.gene004587:CDS 35.0%
! GTTGATGCCTTTAAGAAATG+GGG - chr2.2:52132547-52132566 MS.gene004587:CDS 35.0%
! TTTAGGGTTTCATCCTTCAT+TGG - chr2.2:52132462-52132481 MS.gene004587:CDS 35.0%
!! AAAGCCATGGTTTTTGAAGA+AGG + chr2.2:52132029-52132048 None:intergenic 35.0%
!! AGAAGTGTTGGTTCAAAAGA+GGG + chr2.2:52131838-52131857 None:intergenic 35.0%
!! TTGGTAGTTTGCTTAAGTCA+AGG - chr2.2:52132386-52132405 MS.gene004587:CDS 35.0%
!!! ATCCTTCATTGGCTACTTTT+GGG - chr2.2:52132473-52132492 MS.gene004587:CDS 35.0%
!!! CTTTCCCAGAATGTTGTTTT+AGG - chr2.2:52131773-52131792 MS.gene004587:CDS 35.0%
!!! GAGTTTTTGGGTCAATCAAT+TGG - chr2.2:52132654-52132673 MS.gene004587:CDS 35.0%
AACTGAATCAGGCTTATCTG+GGG + chr2.2:52132002-52132021 None:intergenic 40.0%
ACAAAGAATGGCATCCTTGT+TGG + chr2.2:52132269-52132288 None:intergenic 40.0%
GAATTCCTATCACCTTACTG+TGG - chr2.2:52132318-52132337 MS.gene004587:CDS 40.0%
GGAGAAAAGGATCATTCCAA+GGG - chr2.2:52132729-52132748 MS.gene004587:CDS 40.0%
GGTTGTGATGAAAGTAACGA+AGG + chr2.2:52132088-52132107 None:intergenic 40.0%
GTTGCAGCCTAATTTGATGT+TGG - chr2.2:52132603-52132622 MS.gene004587:CDS 40.0%
TATTGAAACGAACCTGCTTG+AGG + chr2.2:52131979-52131998 None:intergenic 40.0%
TGGAGAAAAGGATCATTCCA+AGG - chr2.2:52132728-52132747 MS.gene004587:CDS 40.0%
TGGTAGTTTGATAAGTGCCA+AGG + chr2.2:52132689-52132708 None:intergenic 40.0%
TTTGGGTCAATCAATTGGGT+TGG - chr2.2:52132659-52132678 MS.gene004587:CDS 40.0%
! AAGAGGGTTGTTGAGTTTTG+GGG + chr2.2:52131822-52131841 None:intergenic 40.0%
!! ATCAGATGAAGAAGCACCTT+TGG + chr2.2:52132149-52132168 None:intergenic 40.0%
!! CATCCTTCATTGGCTACTTT+TGG - chr2.2:52132472-52132491 MS.gene004587:CDS 40.0%
!! GAGAAGTGTTGGTTCAAAAG+AGG + chr2.2:52131839-52131858 None:intergenic 40.0%
!! TCAATTGGGTTGGAATTCCT+TGG - chr2.2:52132669-52132688 MS.gene004587:CDS 40.0%
!! TTCAGCTTTAAGAAGGAGTC+TGG - chr2.2:52132856-52132875 MS.gene004587:CDS 40.0%
!!! TCCTTCATTGGCTACTTTTG+GGG - chr2.2:52132474-52132493 MS.gene004587:CDS 40.0%
AATTGCATAACTGAGGCCCT+TGG + chr2.2:52132748-52132767 None:intergenic 45.0%
ACCCCAAAAGTAGCCAATGA+AGG + chr2.2:52132478-52132497 None:intergenic 45.0%
ACTCAGAAGCTTCTGCACAT+CGG - chr2.2:52131871-52131890 MS.gene004587:CDS 45.0%
AGAAAATGGTGCAGCTAAGC+TGG + chr2.2:52132803-52132822 None:intergenic 45.0%
AGACCAGCCCCATTTCTTAA+AGG + chr2.2:52132557-52132576 None:intergenic 45.0%
CAAAGCTTTCAACCTCAAGC+AGG - chr2.2:52131964-52131983 MS.gene004587:CDS 45.0%
TATCGGAGTGTGAAAAGCCA+TGG + chr2.2:52132042-52132061 None:intergenic 45.0%
! ATGCCTTTAAGAAATGGGGC+TGG - chr2.2:52132551-52132570 MS.gene004587:CDS 45.0%
!! AAAGCTTTGAGAGCTGACTG+AGG + chr2.2:52131953-52131972 None:intergenic 45.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52131755 52132957 52131755 ID=MS.gene004587
chr2.2 mRNA 52131755 52132957 52131755 ID=MS.gene004587.t1;Parent=MS.gene004587
chr2.2 exon 52131755 52132957 52131755 ID=MS.gene004587.t1.exon1;Parent=MS.gene004587.t1
chr2.2 CDS 52131755 52132957 52131755 ID=cds.MS.gene004587.t1;Parent=MS.gene004587.t1
Gene Sequence

>MS.gene004587

ATGTTGAAGAAACTGTTTCTTTCCCAGAATGTTGTTTTAGGAATCATCACAGCAAAATCAACAACCCCAAAACTCAACAACCCTCTTTTGAACCAACACTTCTCGTTCCTAATTTCACTCAGAAGCTTCTGCACATCGGAATCAGACACACACCCATTTGCTGTATCATACCTCATCAACAATTTTGGTTTCACACCTCAGTCAGCTCTCAAAGCTTTCAACCTCAAGCAGGTTCGTTTCAATACCCCAGATAAGCCTGATTCAGTTATCACCTTCTTCAAAAACCATGGCTTTTCACACTCCGATATACGCATCATCATCAAAAAAACACCTTCGTTACTTTCATCACAACCCCACAATGTTATTTTGCCAAAGTTTCAATTTTTTCTATCCAAAGGTGCTTCTTCATCTGATATTATTTCATTGTTAACTGAAAACCCTAGAATTTTACGCATTAGCTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAGTAGGTTTATGAAAACCAACAAGGATGCCATTCTTTGTTTAATTCGGCATTCGACTGCATTTTCTATGAATTCCTATCACCTTACTGTGGATAATGTCAATTTGATGTCTGATTTTGGAGTTCCTAATAATGTCATTGGTAGTTTGCTTAAGTCAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGTGTTTAGGGTTTCATCCTTCATTGGCTACTTTTGGGGTTGCTTTGAAAGCCAAGAAATGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCTTTAAGAAATGGGGCTGGTCTGATGAAGCTGTTATTCGAACATTTCAGTTGCAGCCTAATTTGATGTTGGTTTCAATTGATAAGATTGATTTGTTGATGAGTTTTTGGGTCAATCAATTGGGTTGGAATTCCTTGGCACTTATCAAACTACCACAAATGTTTTGTTATAGTTTGGAGAAAAGGATCATTCCAAGGGCCTCAGTTATGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCCAGCTTAGCTGCACCATTTTCTTATTCTGAGAAAATATTTTTGAGCAAGTTTGTTTTCAGCTTTAAGAAGGAGTCTGGTTATCTATTAAAGCTATATGAGCAAAAAATGAAACTTGCATACACAATGGAAAACAATGGCATACCATTCACTAATTAG
Protein sequence

>MS.gene004587.t1

MLKKLFLSQNVVLGIITAKSTTPKLNNPLLNQHFSFLISLRSFCTSESDTHPFAVSYLINNFGFTPQSALKAFNLKQVRFNTPDKPDSVITFFKNHGFSHSDIRIIIKKTPSLLSSQPHNVILPKFQFFLSKGASSSDIISLLTENPRILRISLEKRIIPLFELLSRFMKTNKDAILCLIRHSTAFSMNSYHLTVDNVNLMSDFGVPNNVIGSLLKSRPSIFGSKDLIKSLEEVKCLGFHPSLATFGVALKAKKCLSKKLWDEKVDAFKKWGWSDEAVIRTFQLQPNLMLVSIDKIDLLMSFWVNQLGWNSLALIKLPQMFCYSLEKRIIPRASVMQFLLMKGLRKKNASLAAPFSYSEKIFLSKFVFSFKKESGYLLKLYEQKMKLAYTMENNGIPFTN