AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene68900

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68900.t1 MTR_2g436440 84.359 390 40 4 1 370 1 389 0.0 648
MS.gene68900.t1 MTR_2g437060 62.025 395 120 7 1 366 1 394 3.92e-162 460
MS.gene68900.t1 MTR_2g437080 62.953 386 116 6 1 366 1 379 4.31e-161 457
MS.gene68900.t1 MTR_2g437030 62.245 392 125 6 1 370 79 469 3.44e-159 455
MS.gene68900.t1 MTR_2g437020 63.710 372 110 7 21 370 15 383 1.50e-156 445
MS.gene68900.t1 MTR_2g437040 60.769 390 124 6 1 369 1 382 1.08e-152 435
MS.gene68900.t1 MTR_2g436400 61.082 388 115 7 1 360 1 380 3.83e-152 434
MS.gene68900.t1 MTR_2g437120 50.133 377 164 5 17 370 21 396 5.25e-121 356
MS.gene68900.t1 MTR_2g437170 49.734 376 160 7 23 369 27 402 1.95e-112 334
MS.gene68900.t1 MTR_2g437150 48.670 376 164 7 23 369 27 402 1.31e-109 327
MS.gene68900.t1 MTR_2g437100 50.135 371 152 7 20 357 25 395 2.20e-108 323
MS.gene68900.t1 MTR_2g438010 47.967 369 165 5 28 369 47 415 3.56e-108 323
MS.gene68900.t1 MTR_2g437160 46.292 391 181 7 5 369 20 407 1.97e-107 321
MS.gene68900.t1 MTR_2g437960 47.312 372 169 5 25 369 27 398 1.05e-106 319
MS.gene68900.t1 MTR_2g437990 47.581 372 161 6 28 369 27 394 1.64e-106 320
MS.gene68900.t1 MTR_2g437960 47.312 372 169 5 25 369 27 398 2.20e-106 319
MS.gene68900.t1 MTR_2g076320 47.425 369 167 5 28 369 27 395 3.76e-106 318
MS.gene68900.t1 MTR_2g437200 46.736 383 143 7 34 357 18 398 4.08e-106 317
MS.gene68900.t1 MTR_4g119550 46.243 346 163 4 42 366 46 389 3.05e-104 312
MS.gene68900.t1 MTR_2g437130 47.383 363 167 6 29 369 23 383 9.79e-104 311
MS.gene68900.t1 MTR_2g437240 44.935 385 152 6 43 369 28 410 2.19e-102 308
MS.gene68900.t1 MTR_2g437180 45.623 377 176 8 17 369 21 392 2.18e-101 305
MS.gene68900.t1 MTR_2g438020 45.547 393 151 8 36 369 60 448 7.15e-100 305
MS.gene68900.t1 MTR_2g437260 43.200 375 185 8 12 364 5 373 8.37e-98 296
MS.gene68900.t1 MTR_2g437940 44.562 377 154 7 17 369 21 366 9.91e-96 290
MS.gene68900.t1 MTR_2g436460 45.614 342 163 4 40 360 40 379 5.48e-94 286
MS.gene68900.t1 MTR_2g019840 42.448 384 181 9 1 357 1 371 3.37e-85 263
MS.gene68900.t1 MTR_2g019810 42.382 361 177 7 18 357 9 359 7.91e-83 257
MS.gene68900.t1 MTR_4g120380 41.081 370 189 5 7 355 9 370 1.88e-79 248
MS.gene68900.t1 MTR_4g119580 41.520 342 177 4 35 355 31 370 6.21e-79 247
MS.gene68900.t1 MTR_4g120380 40.730 356 182 5 7 341 9 356 1.27e-73 234
MS.gene68900.t1 MTR_2g436370 59.113 203 68 4 1 196 1 195 5.54e-69 216
MS.gene68900.t1 MTR_2g437090 60.479 167 46 3 220 366 1 167 4.39e-57 184
MS.gene68900.t1 MTR_2g437160 45.098 204 89 4 189 369 113 316 1.34e-49 169
MS.gene68900.t1 MTR_4g119570 40.000 230 123 3 127 355 14 229 1.91e-47 161
MS.gene68900.t1 MTR_3g450500 28.000 350 224 7 34 357 25 372 7.03e-40 145
MS.gene68900.t1 MTR_4g007490 28.280 343 217 9 39 355 29 368 4.01e-36 135
MS.gene68900.t1 MTR_4g049400 44.928 138 72 2 13 146 12 149 9.92e-30 114
MS.gene68900.t1 MTR_8g080990 26.786 336 220 7 40 351 35 368 9.39e-24 101
MS.gene68900.t1 MTR_2g436380 48.936 94 36 3 220 305 1 90 9.77e-19 85.9
MS.gene68900.t1 MTR_8g081000 24.351 308 196 7 78 355 85 385 2.41e-18 85.9
MS.gene68900.t1 MTR_8g081000 24.510 306 194 7 80 355 87 385 2.49e-18 86.3
MS.gene68900.t1 MTR_8g081000 24.351 308 196 7 78 355 85 385 2.59e-18 85.9
MS.gene68900.t1 MTR_3g085240 35.669 157 87 4 12 168 27 169 9.11e-16 76.6
MS.gene68900.t1 MTR_4g119615 56.604 53 23 0 296 348 23 75 5.20e-13 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene68900.t1 AT5G07900 31.532 333 200 6 48 354 69 399 1.85e-52 179
MS.gene68900.t1 AT1G21150 30.211 331 203 8 48 352 56 384 2.03e-38 142
MS.gene68900.t1 AT1G21150 30.211 331 203 8 48 352 120 448 5.16e-38 142
MS.gene68900.t1 AT1G21150 29.641 334 207 8 45 352 17 348 8.38e-38 139
MS.gene68900.t1 AT1G61980 27.838 370 198 10 48 354 52 415 7.37e-31 122
MS.gene68900.t1 AT1G61980 27.838 370 198 10 48 354 52 415 7.37e-31 122
MS.gene68900.t1 AT1G61980 27.838 370 198 10 48 354 52 415 7.37e-31 122
MS.gene68900.t1 AT1G61990 27.869 366 194 9 49 354 53 408 5.33e-30 119
MS.gene68900.t1 AT1G61990 27.869 366 194 9 49 354 53 408 5.33e-30 119
MS.gene68900.t1 AT3G46950 32.365 241 151 6 34 270 38 270 3.33e-28 115
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G61970 27.920 351 192 8 48 340 52 399 2.01e-27 112
MS.gene68900.t1 AT1G62120 27.119 354 197 9 48 342 56 407 2.30e-25 106
MS.gene68900.t1 AT1G62085 29.712 313 181 9 25 302 25 333 2.85e-25 106
MS.gene68900.t1 AT1G62085 29.712 313 181 9 25 302 25 333 2.85e-25 106
MS.gene68900.t1 AT1G61960 31.278 227 149 5 48 270 52 275 1.87e-23 101
MS.gene68900.t1 AT1G62110 26.821 302 187 8 30 302 34 330 1.33e-21 96.3
MS.gene68900.t1 AT5G23930 28.571 315 182 10 23 302 20 326 5.23e-21 94.4
MS.gene68900.t1 AT5G64950 26.437 348 211 11 37 346 26 366 8.33e-21 93.2
MS.gene68900.t1 AT1G62010 24.845 322 181 7 83 346 71 389 4.80e-20 91.3
MS.gene68900.t1 AT1G62150 27.953 254 162 8 41 282 51 295 4.00e-18 85.9

Find 93 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTATCTCATTCACAATTT+TGG 0.046895 2.4:+55030317 MS.gene68900:CDS
TAAGAATCATATGACGTAAT+TGG 0.170642 2.4:-55030454 None:intergenic
TGAGAACAGAATCAGGTTTC+TGG 0.201018 2.4:-55030400 None:intergenic
ATTCGAAATTCAACTCTCTT+TGG 0.232842 2.4:+55030689 MS.gene68900:CDS
GTGGAGGAATTAAAGGATTT+GGG 0.286091 2.4:+55030833 MS.gene68900:CDS
CAAGTTGGTGGAGGAATTAA+AGG 0.293374 2.4:+55030826 MS.gene68900:CDS
AAACTTTGGCAAAACCCTTT+TGG 0.296153 2.4:-55030507 None:intergenic
GTTGCAAAAGGTTAGGGTTT+GGG 0.297466 2.4:-55030226 None:intergenic
AGACCAACCCCATTTCTTAA+AGG 0.307244 2.4:-55030939 None:intergenic
GGTGGAGGAATTAAAGGATT+TGG 0.308407 2.4:+55030832 MS.gene68900:CDS
CTCAGTTTCTTTAGAAATCA+TGG 0.318502 2.4:+55030419 MS.gene68900:CDS
GTGCAGAAATGCAAAGAAAA+TGG 0.333884 2.4:-55030260 None:intergenic
ACTAATAACCCTTGGTACTT+TGG 0.349074 2.4:-55031082 None:intergenic
GAGTAATGTTGCAAAAGGTT+AGG 0.357061 2.4:-55030233 None:intergenic
AGTCGATACCTTTAAGAAAT+GGG 0.363719 2.4:+55030931 MS.gene68900:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.4:+55031044 MS.gene68900:intron
GTACACACCAGGGACAAAAT+TGG 0.368393 2.4:+55031300 MS.gene68900:CDS
AAGTCGATACCTTTAAGAAA+TGG 0.369211 2.4:+55030930 MS.gene68900:CDS
AGGTTAGGGTTTGGGGATTG+TGG 0.372461 2.4:-55030218 None:intergenic
AAACAACAAACAAAACCTTA+TGG 0.379559 2.4:+55030900 MS.gene68900:CDS
GGTTAGGGTTTGGGGATTGT+GGG 0.386637 2.4:-55030217 None:intergenic
TATAGTCCCAACCTATGAAT+TGG 0.392822 2.4:+55030622 MS.gene68900:CDS
CTTGATTCTTGCTCGAAGCC+TGG 0.397995 2.4:+55030592 MS.gene68900:CDS
AACGCATTATGTATGTGTTA+AGG 0.404225 2.4:-55031224 None:intergenic
ATCCGTTGAGATAGTAGATT+AGG 0.404365 2.4:-55030187 None:intergenic
AGTAATGTTGCAAAAGGTTA+GGG 0.406209 2.4:-55030232 None:intergenic
TCCAAAATTGTGAATGAGAT+AGG 0.407036 2.4:-55030318 None:intergenic
TACCTCTGACATTGTTAACT+TGG 0.407314 2.4:+55030556 MS.gene68900:CDS
GTGCAATATCTTCTAAAGAA+AGG 0.408077 2.4:+55031144 MS.gene68900:CDS
AAGAATCATATGACGTAATT+GGG 0.408823 2.4:-55030453 None:intergenic
ATTACGTCATATGATTCTTA+AGG 0.409783 2.4:+55030457 MS.gene68900:CDS
TGTTGCAAAAGGTTAGGGTT+TGG 0.412273 2.4:-55030227 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.4:-55030543 None:intergenic
TTATAGATTCTTGCAATCTC+AGG 0.418358 2.4:+55030646 MS.gene68900:CDS
ATCGACTTTCTCTTTCCATA+AGG 0.444594 2.4:-55030915 None:intergenic
TTGCAAAAGGTTAGGGTTTG+GGG 0.444897 2.4:-55030225 None:intergenic
GACATCTAATATCAGATTGC+TGG 0.446468 2.4:+55030724 MS.gene68900:CDS
GAGAACAGAATCAGGTTTCT+GGG 0.446980 2.4:-55030399 None:intergenic
AAGAGTTTAGAAGCAAATTG+TGG 0.449537 2.4:-55030347 None:intergenic
GAATCTATAAACCAATTCAT+AGG 0.454188 2.4:-55030633 None:intergenic
ATCAGATTGCTGGTTGAGAA+TGG 0.454469 2.4:+55030734 MS.gene68900:CDS
AAGAAACTGAGAACAGAATC+AGG 0.455801 2.4:-55030407 None:intergenic
TGGTAAGATTGCTTCGGGAC+TGG 0.458406 2.4:+55030774 MS.gene68900:CDS
ATTGCTTCGGGACTGGAGTC+AGG 0.459523 2.4:+55030781 MS.gene68900:CDS
TCCGTTGAGATAGTAGATTA+GGG 0.460573 2.4:-55030186 None:intergenic
CAACATGGTAAGATTGCTTC+GGG 0.463456 2.4:+55030769 MS.gene68900:CDS
TATAAACCAATTCATAGGTT+GGG 0.464451 2.4:-55030628 None:intergenic
CCAACATGGTAAGATTGCTT+CGG 0.473055 2.4:+55030768 MS.gene68900:CDS
AGAGTTTAGAAGCAAATTGT+GGG 0.475365 2.4:-55030346 None:intergenic
GACTTATCAGTCATTACAAA+TGG 0.480807 2.4:-55031198 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.4:+55031043 MS.gene68900:intron
TTTCCTGCGACCCCTCCAAA+AGG 0.486453 2.4:+55030492 MS.gene68900:CDS
CAGGCTTCGAGCAAGAATCA+AGG 0.503879 2.4:-55030591 None:intergenic
GTTGAAGCGAACATTGTAAG+AGG 0.506885 2.4:-55030372 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.4:+55031053 MS.gene68900:intron
CTATAAACCAATTCATAGGT+TGG 0.510197 2.4:-55030629 None:intergenic
GCAGTGAGTAATGTTGCAAA+AGG 0.511747 2.4:-55030238 None:intergenic
TCAGATTGCTGGTTGAGAAT+GGG 0.517775 2.4:+55030735 MS.gene68900:CDS
ATTTCGAATTACACAAGCAA+TGG 0.525058 2.4:-55030675 None:intergenic
AACCCTTGGTACTTTGGCAA+TGG 0.532796 2.4:-55031076 None:intergenic
ATCTTCTAAAGAAAGGTTTG+CGG 0.535884 2.4:+55031151 MS.gene68900:CDS
TTCCTGCGACCCCTCCAAAA+GGG 0.539011 2.4:+55030493 MS.gene68900:CDS
ACATGACATGCTCAAGTTGG+TGG 0.540844 2.4:+55030814 MS.gene68900:CDS
AGCTGAATCTTGTACACACC+AGG 0.549695 2.4:+55031289 MS.gene68900:CDS
AGATAGGACACAACAAATGA+AGG 0.552393 2.4:-55030302 None:intergenic
GAAATTCAACTCTCTTTGGA+CGG 0.554145 2.4:+55030693 MS.gene68900:CDS
CCGAAGCAATCTTACCATGT+TGG 0.556885 2.4:-55030768 None:intergenic
CACACATGACATGCTCAAGT+TGG 0.571343 2.4:+55030811 MS.gene68900:CDS
GTCGATACCTTTAAGAAATG+GGG 0.575626 2.4:+55030932 MS.gene68900:CDS
AGGCTTCGAGCAAGAATCAA+GGG 0.577197 2.4:-55030590 None:intergenic
CCAGAAAATTATCATACCTA+GGG 0.587562 2.4:+55031113 MS.gene68900:CDS
AGACTACAACTAATAACCCT+TGG 0.592723 2.4:-55031090 None:intergenic
ACCCTAATCTACTATCTCAA+CGG 0.599089 2.4:+55030185 MS.gene68900:CDS
ATCAGTCATTACAAATGGAG+TGG 0.601136 2.4:-55031193 None:intergenic
GGGTCGCAGGAAAGTAGCCA+TGG 0.602735 2.4:-55030482 None:intergenic
CAATCTTACCATGTTGGAGT+CGG 0.603555 2.4:-55030762 None:intergenic
TGACATGCTCAAGTTGGTGG+AGG 0.604351 2.4:+55030817 MS.gene68900:CDS
TCCAGAAAATTATCATACCT+AGG 0.606904 2.4:+55031112 MS.gene68900:CDS
GGCCATTGCCAAAGTACCAA+GGG 0.609457 2.4:+55031074 MS.gene68900:CDS
TGGAGGAATTAAAGGATTTG+GGG 0.615469 2.4:+55030834 MS.gene68900:CDS
ATATTGCTGAAGCATTAGAA+AGG 0.618724 2.4:+55030969 MS.gene68900:CDS
ATACCTTTAAGAAATGGGGT+TGG 0.619145 2.4:+55030936 MS.gene68900:CDS
TATTGCACAACTGAGGCCCT+AGG 0.619185 2.4:-55031129 None:intergenic
CAGATTGCTGGTTGAGAATG+GGG 0.619528 2.4:+55030736 MS.gene68900:CDS
TAGAAGATATTGCACAACTG+AGG 0.636070 2.4:-55031136 None:intergenic
TGGCCATTGCCAAAGTACCA+AGG 0.638748 2.4:+55031073 MS.gene68900:CDS
GAGTTTAGAAGCAAATTGTG+GGG 0.642150 2.4:-55030345 None:intergenic
GACCAAGTTAACAATGTCAG+AGG 0.654821 2.4:-55030558 None:intergenic
TGGGGTGACCGACTCCAACA+TGG 0.675530 2.4:+55030754 MS.gene68900:CDS
GGCTTCGAGCAAGAATCAAG+GGG 0.680008 2.4:-55030589 None:intergenic
ATATGATTCTTAAGGCACCA+TGG 0.707927 2.4:+55030465 MS.gene68900:CDS
GCTGAATCTTGTACACACCA+GGG 0.712860 2.4:+55031290 MS.gene68900:CDS
ACTTGAGCATGTCATGTGTG+CGG 0.743070 2.4:-55030808 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG - chr2.4:55031016-55031035 None:intergenic 15.0%
!!! ATAACAAAAGATGTTTTTGA+AGG - chr2.4:55030866-55030885 None:intergenic 20.0%
!!! TAATTTAGTGATGAACTTTT+GGG + chr2.4:55031026-55031045 MS.gene68900:intron 20.0%
!!! TTAATTTAGTGATGAACTTT+TGG + chr2.4:55031025-55031044 MS.gene68900:intron 20.0%
! AAACAACAAACAAAACCTTA+TGG + chr2.4:55030900-55030919 MS.gene68900:CDS 25.0%
! AAGAATCATATGACGTAATT+GGG - chr2.4:55030456-55030475 None:intergenic 25.0%
! ATTACGTCATATGATTCTTA+AGG + chr2.4:55030457-55030476 MS.gene68900:CDS 25.0%
! GAATCTATAAACCAATTCAT+AGG - chr2.4:55030636-55030655 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG - chr2.4:55030524-55030543 None:intergenic 25.0%
! TAAGAATCATATGACGTAAT+TGG - chr2.4:55030457-55030476 None:intergenic 25.0%
! TATAAACCAATTCATAGGTT+GGG - chr2.4:55030631-55030650 None:intergenic 25.0%
!!! ATGGATTTTAACATACAATG+AGG - chr2.4:55030997-55031016 None:intergenic 25.0%
!!! TAAGGTTTTGTTTGTTGTTT+TGG - chr2.4:55030900-55030919 None:intergenic 25.0%
!!! TGGATTTTAACATACAATGA+GGG - chr2.4:55030996-55031015 None:intergenic 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG + chr2.4:55030527-55030546 MS.gene68900:CDS 25.0%
AACGCATTATGTATGTGTTA+AGG - chr2.4:55031227-55031246 None:intergenic 30.0%
AAGAGTTTAGAAGCAAATTG+TGG - chr2.4:55030350-55030369 None:intergenic 30.0%
AGAGTTTAGAAGCAAATTGT+GGG - chr2.4:55030349-55030368 None:intergenic 30.0%
AGTAATGTTGCAAAAGGTTA+GGG - chr2.4:55030235-55030254 None:intergenic 30.0%
ATTCGAAATTCAACTCTCTT+TGG + chr2.4:55030689-55030708 MS.gene68900:CDS 30.0%
ATTTCGAATTACACAAGCAA+TGG - chr2.4:55030678-55030697 None:intergenic 30.0%
CCAGAAAATTATCATACCTA+GGG + chr2.4:55031113-55031132 MS.gene68900:CDS 30.0%
CTATAAACCAATTCATAGGT+TGG - chr2.4:55030632-55030651 None:intergenic 30.0%
GACTTATCAGTCATTACAAA+TGG - chr2.4:55031201-55031220 None:intergenic 30.0%
TCCAAAATTGTGAATGAGAT+AGG - chr2.4:55030321-55030340 None:intergenic 30.0%
TCCAGAAAATTATCATACCT+AGG + chr2.4:55031112-55031131 MS.gene68900:CDS 30.0%
TTATAGATTCTTGCAATCTC+AGG + chr2.4:55030646-55030665 MS.gene68900:CDS 30.0%
! AAGTCGATACCTTTAAGAAA+TGG + chr2.4:55030930-55030949 MS.gene68900:CDS 30.0%
! AGTCGATACCTTTAAGAAAT+GGG + chr2.4:55030931-55030950 MS.gene68900:CDS 30.0%
! ATATTGCTGAAGCATTAGAA+AGG + chr2.4:55030969-55030988 MS.gene68900:CDS 30.0%
! ATCTTCTAAAGAAAGGTTTG+CGG + chr2.4:55031151-55031170 MS.gene68900:CDS 30.0%
! CACATACATAATGCGTTTTA+AGG + chr2.4:55031230-55031249 MS.gene68900:CDS 30.0%
! CTCAGTTTCTTTAGAAATCA+TGG + chr2.4:55030419-55030438 MS.gene68900:CDS 30.0%
! GTGCAATATCTTCTAAAGAA+AGG + chr2.4:55031144-55031163 MS.gene68900:CDS 30.0%
! TCCTATCTCATTCACAATTT+TGG + chr2.4:55030317-55030336 MS.gene68900:CDS 30.0%
!!! ATACATAATGCGTTTTAAGG+AGG + chr2.4:55031233-55031252 MS.gene68900:CDS 30.0%
AAGAAACTGAGAACAGAATC+AGG - chr2.4:55030410-55030429 None:intergenic 35.0%
ACCCTAATCTACTATCTCAA+CGG + chr2.4:55030185-55030204 MS.gene68900:CDS 35.0%
ACTAATAACCCTTGGTACTT+TGG - chr2.4:55031085-55031104 None:intergenic 35.0%
AGACTACAACTAATAACCCT+TGG - chr2.4:55031093-55031112 None:intergenic 35.0%
AGATAGGACACAACAAATGA+AGG - chr2.4:55030305-55030324 None:intergenic 35.0%
ATCAGTCATTACAAATGGAG+TGG - chr2.4:55031196-55031215 None:intergenic 35.0%
ATCCGTTGAGATAGTAGATT+AGG - chr2.4:55030190-55030209 None:intergenic 35.0%
GAAATTCAACTCTCTTTGGA+CGG + chr2.4:55030693-55030712 MS.gene68900:CDS 35.0%
GACATCTAATATCAGATTGC+TGG + chr2.4:55030724-55030743 MS.gene68900:CDS 35.0%
GAGTAATGTTGCAAAAGGTT+AGG - chr2.4:55030236-55030255 None:intergenic 35.0%
GAGTTTAGAAGCAAATTGTG+GGG - chr2.4:55030348-55030367 None:intergenic 35.0%
GTGCAGAAATGCAAAGAAAA+TGG - chr2.4:55030263-55030282 None:intergenic 35.0%
GTGGAGGAATTAAAGGATTT+GGG + chr2.4:55030833-55030852 MS.gene68900:CDS 35.0%
TACCTCTGACATTGTTAACT+TGG + chr2.4:55030556-55030575 MS.gene68900:CDS 35.0%
TAGAAGATATTGCACAACTG+AGG - chr2.4:55031139-55031158 None:intergenic 35.0%
TATAGTCCCAACCTATGAAT+TGG + chr2.4:55030622-55030641 MS.gene68900:CDS 35.0%
TCCGTTGAGATAGTAGATTA+GGG - chr2.4:55030189-55030208 None:intergenic 35.0%
TGGAGGAATTAAAGGATTTG+GGG + chr2.4:55030834-55030853 MS.gene68900:CDS 35.0%
! AAACTTTGGCAAAACCCTTT+TGG - chr2.4:55030510-55030529 None:intergenic 35.0%
! ATACCTTTAAGAAATGGGGT+TGG + chr2.4:55030936-55030955 MS.gene68900:CDS 35.0%
! ATATGATTCTTAAGGCACCA+TGG + chr2.4:55030465-55030484 MS.gene68900:CDS 35.0%
! ATCGACTTTCTCTTTCCATA+AGG - chr2.4:55030918-55030937 None:intergenic 35.0%
! CCCTAGGTATGATAATTTTC+TGG - chr2.4:55031116-55031135 None:intergenic 35.0%
! GTCGATACCTTTAAGAAATG+GGG + chr2.4:55030932-55030951 MS.gene68900:CDS 35.0%
! TTGGGACTATATGATTTTCC+AGG - chr2.4:55030613-55030632 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG + chr2.4:55031039-55031058 MS.gene68900:intron 35.0%
AGACCAACCCCATTTCTTAA+AGG - chr2.4:55030942-55030961 None:intergenic 40.0%
CAACATGGTAAGATTGCTTC+GGG + chr2.4:55030769-55030788 MS.gene68900:CDS 40.0%
CAATCTTACCATGTTGGAGT+CGG - chr2.4:55030765-55030784 None:intergenic 40.0%
CCAACATGGTAAGATTGCTT+CGG + chr2.4:55030768-55030787 MS.gene68900:CDS 40.0%
GACCAAGTTAACAATGTCAG+AGG - chr2.4:55030561-55030580 None:intergenic 40.0%
GAGAACAGAATCAGGTTTCT+GGG - chr2.4:55030402-55030421 None:intergenic 40.0%
GCAGTGAGTAATGTTGCAAA+AGG - chr2.4:55030241-55030260 None:intergenic 40.0%
GGTGGAGGAATTAAAGGATT+TGG + chr2.4:55030832-55030851 MS.gene68900:CDS 40.0%
GTTGAAGCGAACATTGTAAG+AGG - chr2.4:55030375-55030394 None:intergenic 40.0%
GTTGCAAAAGGTTAGGGTTT+GGG - chr2.4:55030229-55030248 None:intergenic 40.0%
TGAGAACAGAATCAGGTTTC+TGG - chr2.4:55030403-55030422 None:intergenic 40.0%
TGTTGCAAAAGGTTAGGGTT+TGG - chr2.4:55030230-55030249 None:intergenic 40.0%
TTGCAAAAGGTTAGGGTTTG+GGG - chr2.4:55030228-55030247 None:intergenic 40.0%
! ATCAGATTGCTGGTTGAGAA+TGG + chr2.4:55030734-55030753 MS.gene68900:CDS 40.0%
! TCAGATTGCTGGTTGAGAAT+GGG + chr2.4:55030735-55030754 MS.gene68900:CDS 40.0%
! TTTGGCAAAACCCTTTTGGA+GGG - chr2.4:55030506-55030525 None:intergenic 40.0%
!! CAAGTTGGTGGAGGAATTAA+AGG + chr2.4:55030826-55030845 MS.gene68900:CDS 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG + chr2.4:55031038-55031057 MS.gene68900:intron 40.0%
AACCCTTGGTACTTTGGCAA+TGG - chr2.4:55031079-55031098 None:intergenic 45.0%
ACTTGAGCATGTCATGTGTG+CGG - chr2.4:55030811-55030830 None:intergenic 45.0%
AGCTGAATCTTGTACACACC+AGG + chr2.4:55031289-55031308 MS.gene68900:CDS 45.0%
AGGCTTCGAGCAAGAATCAA+GGG - chr2.4:55030593-55030612 None:intergenic 45.0%
CACACATGACATGCTCAAGT+TGG + chr2.4:55030811-55030830 MS.gene68900:CDS 45.0%
CCGAAGCAATCTTACCATGT+TGG - chr2.4:55030771-55030790 None:intergenic 45.0%
GCTGAATCTTGTACACACCA+GGG + chr2.4:55031290-55031309 MS.gene68900:CDS 45.0%
GTACACACCAGGGACAAAAT+TGG + chr2.4:55031300-55031319 MS.gene68900:CDS 45.0%
TTGGGTCAATCAGTTGGGTT+GGG + chr2.4:55031044-55031063 MS.gene68900:intron 45.0%
TTTGGGTCAATCAGTTGGGT+TGG + chr2.4:55031043-55031062 MS.gene68900:intron 45.0%
! CAGATTGCTGGTTGAGAATG+GGG + chr2.4:55030736-55030755 MS.gene68900:CDS 45.0%
! CTTTGGCAAAACCCTTTTGG+AGG - chr2.4:55030507-55030526 None:intergenic 45.0%
! TGACATGCCAATTTTGTCCC+TGG - chr2.4:55031310-55031329 None:intergenic 45.0%
!! ACATGACATGCTCAAGTTGG+TGG + chr2.4:55030814-55030833 MS.gene68900:CDS 45.0%
!! TTGGCAAAACCCTTTTGGAG+GGG - chr2.4:55030505-55030524 None:intergenic 45.0%
AGGTTAGGGTTTGGGGATTG+TGG - chr2.4:55030221-55030240 None:intergenic 50.0%
CAGGCTTCGAGCAAGAATCA+AGG - chr2.4:55030594-55030613 None:intergenic 50.0%
CTTGATTCTTGCTCGAAGCC+TGG + chr2.4:55030592-55030611 MS.gene68900:CDS 50.0%
GGCTTCGAGCAAGAATCAAG+GGG - chr2.4:55030592-55030611 None:intergenic 50.0%
GGTTAGGGTTTGGGGATTGT+GGG - chr2.4:55030220-55030239 None:intergenic 50.0%
TATTGCACAACTGAGGCCCT+AGG - chr2.4:55031132-55031151 None:intergenic 50.0%
TGGTAAGATTGCTTCGGGAC+TGG + chr2.4:55030774-55030793 MS.gene68900:CDS 50.0%
! GGCCATTGCCAAAGTACCAA+GGG + chr2.4:55031074-55031093 MS.gene68900:CDS 50.0%
! TGGCCATTGCCAAAGTACCA+AGG + chr2.4:55031073-55031092 MS.gene68900:CDS 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG - chr2.4:55030546-55030565 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG + chr2.4:55031053-55031072 MS.gene68900:intron 50.0%
!! TGACATGCTCAAGTTGGTGG+AGG + chr2.4:55030817-55030836 MS.gene68900:CDS 50.0%
ATTGCTTCGGGACTGGAGTC+AGG + chr2.4:55030781-55030800 MS.gene68900:CDS 55.0%
TTCCTGCGACCCCTCCAAAA+GGG + chr2.4:55030493-55030512 MS.gene68900:CDS 55.0%
TTTCCTGCGACCCCTCCAAA+AGG + chr2.4:55030492-55030511 MS.gene68900:CDS 55.0%
GGGTCGCAGGAAAGTAGCCA+TGG - chr2.4:55030485-55030504 None:intergenic 60.0%
! TGGGGTGACCGACTCCAACA+TGG + chr2.4:55030754-55030773 MS.gene68900:CDS 60.0%
!! AACCCTTTTGGAGGGGTCGC+AGG - chr2.4:55030498-55030517 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 55030164 55031332 55030164 ID=MS.gene68900
chr2.4 mRNA 55030164 55031332 55030164 ID=MS.gene68900.t1;Parent=MS.gene68900
chr2.4 exon 55030164 55031000 55030164 ID=MS.gene68900.t1.exon1;Parent=MS.gene68900.t1
chr2.4 CDS 55030164 55031000 55030164 ID=cds.MS.gene68900.t1;Parent=MS.gene68900.t1
chr2.4 exon 55031057 55031332 55031057 ID=MS.gene68900.t1.exon2;Parent=MS.gene68900.t1
chr2.4 CDS 55031057 55031332 55031057 ID=cds.MS.gene68900.t1;Parent=MS.gene68900.t1
Gene Sequence

>MS.gene68900

ATGTTCAAGTTCAACAGAAAGACCCTAATCTACTATCTCAACGGATTAACTTTCCCACAATCCCCAAACCCTAACCTTTTGCAACATTACTCACTGCCATTTTCTTTGCATTTCTGCACTAAAACTTCAGCAGATTCACCTTCATTTGTTGTGTCCTATCTCATTCACAATTTTGGATTCACCCCACAATTTGCTTCTAAACTCTTCTCCTCTTACAATGTTCGCTTCAACACTTCCCAGAAACCTGATTCTGTTCTCAGTTTCTTTAGAAATCATGGTTTCTCTGATTCCCAATTACGTCATATGATTCTTAAGGCACCATGGCTACTTTCCTGCGACCCCTCCAAAAGGGTTTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATTGTTAACTTGGTCAGTAAGAGCCCCTTGATTCTTGCTCGAAGCCTGGAAAATCATATAGTCCCAACCTATGAATTGGTTTATAGATTCTTGCAATCTCAGGTTGACACCATTGCTTGTGTAATTCGAAATTCAACTCTCTTTGGACGGAGTCATGTGACATCTAATATCAGATTGCTGGTTGAGAATGGGGTGACCGACTCCAACATGGTAAGATTGCTTCGGGACTGGAGTCAGGTATTCCGCACACATGACATGCTCAAGTTGGTGGAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACATCTTTTGTTATAGCGCTGCTTGCCAAAACAACAAACAAAACCTTATGGAAAGAGAAAGTCGATACCTTTAAGAAATGGGGTTGGTCTGATGAAGATATTGCTGAAGCATTAGAAAGGACCCTCATTGTATGTTAAAATCCATTGATAAAATTAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAAGTACCAAGGGTTATTAGTTGTAGTCTCCAGAAAATTATCATACCTAGGGCCTCAGTTGTGCAATATCTTCTAAAGAAAGGTTTGCGGAAAAAGAATGCAAGCTTAACCACTCCATTTGTAATGACTGATAAGTCGTTCCTTAACACATACATAATGCGTTTTAAGGAGGAGTCATCTTATCTCTTAAAGCTCTATGAAGAAAAGCTGAATCTTGTACACACCAGGGACAAAATTGGCATGTCATGA
Protein sequence

>MS.gene68900.t1

MFKFNRKTLIYYLNGLTFPQSPNPNLLQHYSLPFSLHFCTKTSADSPSFVVSYLIHNFGFTPQFASKLFSSYNVRFNTSQKPDSVLSFFRNHGFSDSQLRHMILKAPWLLSCDPSKRVLPKFQFFLSKGASTSDIVNLVSKSPLILARSLENHIVPTYELVYRFLQSQVDTIACVIRNSTLFGRSHVTSNIRLLVENGVTDSNMVRLLRDWSQVFRTHDMLKLVEELKDLGFNPSKTSFVIALLAKTTNKTLWKEKVDTFKKWGWSDEDIAEALERTLILGWDAMAIAKVPRVISCSLQKIIIPRASVVQYLLKKGLRKKNASLTTPFVMTDKSFLNTYIMRFKEESSYLLKLYEEKLNLVHTRDKIGMS