AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004589

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004589.t1 MTR_2g437170 77.296 392 82 3 15 401 23 412 0.0 604
MS.gene004589.t1 MTR_2g437150 73.890 383 93 3 15 392 23 403 0.0 560
MS.gene004589.t1 MTR_2g437160 70.968 403 106 3 1 395 12 411 0.0 555
MS.gene004589.t1 MTR_2g437960 69.268 410 113 4 1 401 1 406 0.0 533
MS.gene004589.t1 MTR_2g437960 69.287 407 112 4 1 398 1 403 0.0 527
MS.gene004589.t1 MTR_2g437100 69.409 389 109 4 1 379 7 395 0.0 515
MS.gene004589.t1 MTR_2g437180 66.253 403 115 4 1 392 1 393 0.0 509
MS.gene004589.t1 MTR_2g437120 66.915 402 115 4 1 391 1 395 1.53e-179 506
MS.gene004589.t1 MTR_2g438010 65.873 378 125 2 22 399 48 421 1.98e-174 493
MS.gene004589.t1 MTR_2g437990 67.109 377 119 3 22 398 28 399 7.61e-171 484
MS.gene004589.t1 MTR_2g076320 66.667 378 122 2 22 399 28 401 3.17e-170 482
MS.gene004589.t1 MTR_2g437240 61.800 411 110 5 24 399 18 416 1.56e-162 463
MS.gene004589.t1 MTR_2g437200 65.195 385 93 3 30 379 20 398 2.74e-162 461
MS.gene004589.t1 MTR_2g437940 61.707 410 108 5 1 399 1 372 1.56e-161 459
MS.gene004589.t1 MTR_2g437130 60.309 388 134 6 13 392 9 384 2.67e-149 428
MS.gene004589.t1 MTR_2g438020 59.383 389 118 3 45 398 70 453 1.03e-147 428
MS.gene004589.t1 MTR_2g437020 52.672 393 165 5 1 387 1 378 3.67e-126 369
MS.gene004589.t1 MTR_2g437080 50.811 370 169 3 26 391 22 382 1.58e-122 360
MS.gene004589.t1 MTR_2g437060 51.771 367 167 3 21 387 37 393 3.98e-121 357
MS.gene004589.t1 MTR_2g436440 49.745 392 176 5 6 391 12 388 2.07e-120 355
MS.gene004589.t1 MTR_2g437030 52.273 352 165 1 40 391 120 468 8.30e-118 351
MS.gene004589.t1 MTR_2g436400 50.291 344 168 1 40 383 41 381 6.23e-113 335
MS.gene004589.t1 MTR_2g437040 50.420 357 166 3 39 391 33 382 2.35e-112 334
MS.gene004589.t1 MTR_2g437260 46.797 359 180 4 30 386 24 373 5.09e-108 323
MS.gene004589.t1 MTR_4g119550 47.126 348 178 3 41 387 46 388 1.35e-104 315
MS.gene004589.t1 MTR_4g120380 46.921 341 176 2 36 376 34 369 1.50e-99 301
MS.gene004589.t1 MTR_2g437160 74.731 186 47 0 210 395 135 320 9.60e-95 287
MS.gene004589.t1 MTR_2g437160 58.219 146 47 3 1 138 12 151 7.37e-41 147
MS.gene004589.t1 MTR_2g436460 47.522 343 174 4 39 380 40 377 2.12e-94 288
MS.gene004589.t1 MTR_4g120380 47.516 322 164 2 36 357 34 350 8.07e-94 288
MS.gene004589.t1 MTR_4g119580 45.133 339 181 2 38 376 36 369 9.71e-92 281
MS.gene004589.t1 MTR_2g019810 43.027 337 181 6 46 379 31 359 7.68e-78 245
MS.gene004589.t1 MTR_2g019840 40.701 371 198 8 23 379 9 371 1.12e-76 243
MS.gene004589.t1 MTR_2g437090 51.462 171 83 0 222 392 1 171 1.12e-57 186
MS.gene004589.t1 MTR_3g450500 30.328 366 245 5 15 376 10 369 2.82e-50 174
MS.gene004589.t1 MTR_4g119570 38.057 247 117 3 129 375 17 227 4.11e-48 164
MS.gene004589.t1 MTR_4g007490 31.214 346 230 5 35 376 26 367 1.84e-45 161
MS.gene004589.t1 MTR_2g436370 46.497 157 81 2 40 196 40 193 2.86e-38 137
MS.gene004589.t1 MTR_4g049400 48.684 152 70 3 1 146 1 150 7.89e-38 137
MS.gene004589.t1 MTR_8g081000 28.000 300 212 2 79 376 87 384 3.34e-29 117
MS.gene004589.t1 MTR_8g081000 28.000 300 212 2 79 376 87 384 3.61e-29 117
MS.gene004589.t1 MTR_8g081000 28.000 300 212 2 79 376 87 384 4.57e-29 117
MS.gene004589.t1 MTR_8g080990 29.293 297 206 2 79 373 74 368 3.49e-28 114
MS.gene004589.t1 MTR_3g085240 56.522 92 23 1 279 370 174 248 9.16e-23 97.1
MS.gene004589.t1 MTR_3g085240 33.523 176 86 6 30 202 48 195 1.02e-15 77.0
MS.gene004589.t1 MTR_2g436380 60.000 60 24 0 222 281 1 60 3.12e-16 79.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004589.t1 AT5G07900 33.512 373 230 7 16 376 33 399 1.59e-57 193
MS.gene004589.t1 AT1G21150 30.539 334 227 3 44 374 17 348 2.69e-47 165
MS.gene004589.t1 AT1G21150 30.909 330 223 3 48 374 57 384 3.29e-47 166
MS.gene004589.t1 AT1G21150 30.909 330 223 3 48 374 121 448 1.64e-46 166
MS.gene004589.t1 AT5G64950 29.213 356 239 8 30 376 24 375 2.98e-32 125
MS.gene004589.t1 AT1G61980 28.852 357 202 9 48 362 53 399 2.52e-28 115
MS.gene004589.t1 AT1G61980 28.852 357 202 9 48 362 53 399 2.52e-28 115
MS.gene004589.t1 AT1G61980 28.852 357 202 9 48 362 53 399 2.52e-28 115
MS.gene004589.t1 AT3G46950 29.167 312 191 9 25 324 31 324 1.47e-26 110
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G61970 27.042 355 211 8 48 362 53 399 2.60e-26 109
MS.gene004589.t1 AT1G62120 28.182 330 198 5 46 339 55 381 7.32e-26 108
MS.gene004589.t1 AT1G61990 27.083 336 195 6 48 346 53 375 9.93e-26 108
MS.gene004589.t1 AT1G61990 27.083 336 195 6 48 346 53 375 9.93e-26 108
MS.gene004589.t1 AT1G61960 29.167 288 195 6 7 292 14 294 9.72e-23 100
MS.gene004589.t1 AT1G62085 27.245 323 209 10 21 324 18 333 2.40e-22 99.0
MS.gene004589.t1 AT1G62085 27.245 323 209 10 21 324 18 333 2.40e-22 99.0
MS.gene004589.t1 AT1G62110 28.077 260 181 4 35 292 40 295 7.77e-22 97.4
MS.gene004589.t1 AT1G62150 28.163 245 172 3 48 291 59 300 4.47e-20 92.0
MS.gene004589.t1 AT5G23930 26.645 304 199 7 33 324 35 326 7.01e-17 82.4
MS.gene004589.t1 AT1G62010 25.763 295 176 5 82 339 71 359 2.04e-15 77.8

Find 64 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTGAACCATTTCGTTATTC+TGG 0.141446 2.2:-52117189 MS.gene004589:CDS
ATCAATTTAATGTCTGATTT+TGG 0.143567 2.2:-52117651 MS.gene004589:CDS
TCATACCTTATCAACAATTT+TGG 0.165317 2.2:-52118077 MS.gene004589:CDS
TTCAATTGATAAGATTAATT+TGG 0.211307 2.2:-52117370 MS.gene004589:CDS
AAGCTACTTCGTAAAATTCT+AGG 0.214273 2.2:+52117804 None:intergenic
TGGAGGAAGTTAAGAGTTTA+GGG 0.232380 2.2:-52117551 MS.gene004589:CDS
TTGGAGGAAGTTAAGAGTTT+AGG 0.257551 2.2:-52117552 MS.gene004589:CDS
ATTCCTTGGCACTTGCTAAA+AGG 0.292312 2.2:-52117314 MS.gene004589:CDS
AAGAAGGTGAGAACTGAATT+AGG 0.306086 2.2:+52117987 None:intergenic
AAAGGATTTGATTAAGTCAT+TGG 0.323129 2.2:-52117571 MS.gene004589:CDS
AATTGATATTAGACACAATA+AGG 0.329025 2.2:+52117667 None:intergenic
GATCCTTGGACGAATACTTT+TGG 0.330015 2.2:-52117525 MS.gene004589:CDS
CAGTCAAGGCCATTCACATT+TGG 0.332942 2.2:-52117597 MS.gene004589:CDS
TAGACCAACCCCATTTCTTA+AGG 0.338165 2.2:+52117442 None:intergenic
ATTAGACACAATAAGGTGAT+AGG 0.358420 2.2:+52117674 None:intergenic
CAAGTTACACATTCGGTAGA+TGG 0.370763 2.2:+52117048 None:intergenic
AAGTTGATGCCCTTAAGAAA+TGG 0.388575 2.2:-52117452 MS.gene004589:CDS
TAGTATCTGATGAGTGAAAT+CGG 0.388813 2.2:+52118152 None:intergenic
AGAGTTTAGGGTTTGATCCT+TGG 0.397034 2.2:-52117539 MS.gene004589:CDS
AGTTGATGCCCTTAAGAAAT+GGG 0.397811 2.2:-52117451 MS.gene004589:CDS
TTGAACCATTTCGTTATTCT+GGG 0.398296 2.2:-52117188 MS.gene004589:CDS
GCAAACAATGGTATCCTTGT+CGG 0.427133 2.2:+52117731 None:intergenic
AGAAACCAAAATTGTTGATA+AGG 0.433609 2.2:+52118072 None:intergenic
GCCATTCACATTTGGTTCAA+AGG 0.442471 2.2:-52117589 MS.gene004589:CDS
AGACCAACCCCATTTCTTAA+GGG 0.447273 2.2:+52117443 None:intergenic
AACAATTGTTCCAAGGGCAT+TGG 0.458416 2.2:-52117262 MS.gene004589:CDS
AGCTACTTCGTAAAATTCTA+GGG 0.465397 2.2:+52117805 None:intergenic
TCAATTGGGTGGGAATTCCT+TGG 0.472262 2.2:-52117328 MS.gene004589:CDS
TTTCATCACAACCCCACAAA+AGG 0.473339 2.2:-52117902 MS.gene004589:CDS
CTGCAATATCTTGTGATGAA+AGG 0.485086 2.2:-52117237 MS.gene004589:CDS
ATCAGAGGAAGAAGCACCAT+TGG 0.505631 2.2:+52117851 None:intergenic
ATCTTGTGATGAAAGGTTTG+AGG 0.509536 2.2:-52117230 MS.gene004589:CDS
TCCTTTGAACCAAATGTGAA+TGG 0.512045 2.2:+52117588 None:intergenic
TGCAGAAAACAATTGTTCCA+AGG 0.522766 2.2:-52117269 MS.gene004589:CDS
TGTTTAACAAGTTACACATT+CGG 0.526635 2.2:+52117041 None:intergenic
CAAGCATTTAGGATGCATCC+TGG 0.527087 2.2:-52117405 MS.gene004589:CDS
ACACATTCGGTAGATGGCAT+TGG 0.528449 2.2:+52117054 None:intergenic
TTGTTTGCTTAATTCGATGT+TGG 0.530080 2.2:-52117716 MS.gene004589:CDS
GCAGAAAACAATTGTTCCAA+GGG 0.535861 2.2:-52117268 MS.gene004589:CDS
CAAAGCTTTCAACAACAAGC+AGG 0.537532 2.2:-52118033 MS.gene004589:CDS
AGTATCTGATGAGTGAAATC+GGG 0.544517 2.2:+52118153 None:intergenic
GAGACCTAAAACTACATTTG+TGG 0.545867 2.2:+52118204 None:intergenic
AAAGCTTTGAGAGCAGATTG+AGG 0.549590 2.2:+52118047 None:intergenic
TATTGCAGAACCAATGCCCT+TGG 0.552410 2.2:+52117252 None:intergenic
TTTGGGTTAATCAATTGGGT+GGG 0.559000 2.2:-52117338 MS.gene004589:CDS
TCGGGAACAACAAGTGTTGA+GGG 0.560234 2.2:+52118171 None:intergenic
ATCGGGAACAACAAGTGTTG+AGG 0.562201 2.2:+52118170 None:intergenic
TTAGACACAATAAGGTGATA+GGG 0.570881 2.2:+52117675 None:intergenic
GGATTTGATTAAGTCATTGG+AGG 0.571043 2.2:-52117568 MS.gene004589:CDS
CTCACCTTCTTAGAAAACCA+TGG 0.576168 2.2:-52117975 MS.gene004589:CDS
TAAGGTATGATACAGCGAAT+GGG 0.582706 2.2:+52118090 None:intergenic
TTGCTAGCTCGCTTCAGTCA+AGG 0.593225 2.2:-52117611 MS.gene004589:CDS
ATAAGGTATGATACAGCGAA+TGG 0.604855 2.2:+52118089 None:intergenic
ATTCCAAAAGTATTCGTCCA+AGG 0.607263 2.2:+52117522 None:intergenic
GAGAAACAATTTCTTCAACA+TGG 0.614078 2.2:+52118229 None:intergenic
TAACGATGAAACAATATCAG+AGG 0.619380 2.2:+52117836 None:intergenic
ATGCCCTTAAGAAATGGGGT+TGG 0.630132 2.2:-52117446 MS.gene004589:CDS
ACCTAAAACTACATTTGTGG+CGG 0.636797 2.2:+52118207 None:intergenic
GTTGATGCCCTTAAGAAATG+GGG 0.641680 2.2:-52117450 MS.gene004589:CDS
ATTGAAGTCAACATGAAACC+AGG 0.642061 2.2:+52117387 None:intergenic
ACATCGAATTAAGCAAACAA+TGG 0.642451 2.2:+52117719 None:intergenic
TAGGTTCTTTAAAACCGACA+AGG 0.672846 2.2:-52117745 MS.gene004589:CDS
GCATACACAAAAGAGAACAA+TGG 0.682398 2.2:-52117081 MS.gene004589:CDS
GGTTGTGATGAAAGTAACCA+AGG 0.698597 2.2:+52117912 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTTAACAATTGAAAAAGA+GGG + chr2.2:52117508-52117527 None:intergenic 15.0%
!! TTCAATTGATAAGATTAATT+TGG - chr2.2:52117901-52117920 MS.gene004589:CDS 15.0%
!! AAACTTGAATCAAAAATATG+TGG + chr2.2:52117983-52118002 None:intergenic 20.0%
!! AAAGTTTGAGTAAAAAACTT+TGG - chr2.2:52117789-52117808 MS.gene004589:CDS 20.0%
!! AAGTTTGAGTAAAAAACTTT+GGG - chr2.2:52117790-52117809 MS.gene004589:CDS 20.0%
!! AATTGATATTAGACACAATA+AGG + chr2.2:52117607-52117626 None:intergenic 20.0%
!! CTATTTAACAATTGAAAAAG+AGG + chr2.2:52117509-52117528 None:intergenic 20.0%
!! GATAGAAAAAATTGAAACTT+TGG + chr2.2:52117401-52117420 None:intergenic 20.0%
!!! ATCAATTTAATGTCTGATTT+TGG - chr2.2:52117620-52117639 MS.gene004589:CDS 20.0%
!!! CTCTTTTTCAATTGTTAAAT+AGG - chr2.2:52117507-52117526 MS.gene004589:CDS 20.0%
!!! TTTCAATTTTTTCTATCCAA+TGG - chr2.2:52117404-52117423 MS.gene004589:CDS 20.0%
! AAAGGATTTGATTAAGTCAT+TGG - chr2.2:52117700-52117719 MS.gene004589:CDS 25.0%
! AGAAACCAAAATTGTTGATA+AGG + chr2.2:52117202-52117221 None:intergenic 25.0%
!! TCATACCTTATCAACAATTT+TGG - chr2.2:52117194-52117213 MS.gene004589:CDS 25.0%
!!! AGTTTTTGGGTTAATCAATT+GGG - chr2.2:52117929-52117948 MS.gene004589:CDS 25.0%
!!! TAATTTGGTGATGAGTTTTT+GGG - chr2.2:52117916-52117935 MS.gene004589:CDS 25.0%
!!! TTAATTTGGTGATGAGTTTT+TGG - chr2.2:52117915-52117934 MS.gene004589:CDS 25.0%
AAGCTACTTCGTAAAATTCT+AGG + chr2.2:52117470-52117489 None:intergenic 30.0%
ACATCGAATTAAGCAAACAA+TGG + chr2.2:52117555-52117574 None:intergenic 30.0%
AGCTACTTCGTAAAATTCTA+GGG + chr2.2:52117469-52117488 None:intergenic 30.0%
ATTAGACACAATAAGGTGAT+AGG + chr2.2:52117600-52117619 None:intergenic 30.0%
TAACGATGAAACAATATCAG+AGG + chr2.2:52117438-52117457 None:intergenic 30.0%
TAGTATCTGATGAGTGAAAT+CGG + chr2.2:52117122-52117141 None:intergenic 30.0%
TCATCATCAAAAAAGAACCT+TGG - chr2.2:52117342-52117361 MS.gene004589:CDS 30.0%
TTAGACACAATAAGGTGATA+GGG + chr2.2:52117599-52117618 None:intergenic 30.0%
TTGAACCATTTCGTTATTCT+GGG - chr2.2:52118083-52118102 MS.gene004589:CDS 30.0%
TTGTTTGCTTAATTCGATGT+TGG - chr2.2:52117555-52117574 MS.gene004589:CDS 30.0%
! AACTGAATTAGGCTTTTCAA+GGG + chr2.2:52117276-52117295 None:intergenic 30.0%
! ATTTTCCCAGAATAACGAAA+TGG + chr2.2:52118091-52118110 None:intergenic 30.0%
! TAGAATTTTACGAAGTAGCT+TGG - chr2.2:52117469-52117488 MS.gene004589:CDS 30.0%
!! AAAACCATGGTTTTCTAAGA+AGG + chr2.2:52117303-52117322 None:intergenic 30.0%
!! CAAGTTTGTTTTCAGCTTTA+AGG - chr2.2:52118120-52118139 MS.gene004589:CDS 30.0%
!!! AAGCTGTTTTTCAAGCATTT+AGG - chr2.2:52117855-52117874 MS.gene004589:CDS 30.0%
!!! GAATACTTTTGGAATTGCTT+TGG - chr2.2:52117757-52117776 MS.gene004589:CDS 30.0%
!!! GAGTTTTTGGGTTAATCAAT+TGG - chr2.2:52117928-52117947 MS.gene004589:CDS 30.0%
AAGAAGGTGAGAACTGAATT+AGG + chr2.2:52117287-52117306 None:intergenic 35.0%
ACCTAAAACTACATTTGTGG+CGG + chr2.2:52117067-52117086 None:intergenic 35.0%
AGTATCTGATGAGTGAAATC+GGG + chr2.2:52117121-52117140 None:intergenic 35.0%
ATAAGGTATGATACAGCGAA+TGG + chr2.2:52117185-52117204 None:intergenic 35.0%
ATCTTGTGATGAAAGGTTTG+AGG - chr2.2:52118041-52118060 MS.gene004589:CDS 35.0%
ATTGAAGTCAACATGAAACC+AGG + chr2.2:52117887-52117906 None:intergenic 35.0%
CTGCAATATCTTGTGATGAA+AGG - chr2.2:52118034-52118053 MS.gene004589:CDS 35.0%
GAGACCTAAAACTACATTTG+TGG + chr2.2:52117070-52117089 None:intergenic 35.0%
GCAGAAAACAATTGTTCCAA+GGG - chr2.2:52118003-52118022 MS.gene004589:CDS 35.0%
GCATACACAAAAGAGAACAA+TGG - chr2.2:52118190-52118209 MS.gene004589:CDS 35.0%
GGATTTGATTAAGTCATTGG+AGG - chr2.2:52117703-52117722 MS.gene004589:CDS 35.0%
GTTGAACCATTTCGTTATTC+TGG - chr2.2:52118082-52118101 MS.gene004589:CDS 35.0%
TAAGGTATGATACAGCGAAT+GGG + chr2.2:52117184-52117203 None:intergenic 35.0%
TCCTTTGAACCAAATGTGAA+TGG + chr2.2:52117686-52117705 None:intergenic 35.0%
TGCAGAAAACAATTGTTCCA+AGG - chr2.2:52118002-52118021 MS.gene004589:CDS 35.0%
TGGAGGAAGTTAAGAGTTTA+GGG - chr2.2:52117720-52117739 MS.gene004589:CDS 35.0%
TTGGAGGAAGTTAAGAGTTT+AGG - chr2.2:52117719-52117738 MS.gene004589:CDS 35.0%
! ACTTTGGCAATAACCTTTTG+TGG + chr2.2:52117385-52117404 None:intergenic 35.0%
! AGTTGATGCCCTTAAGAAAT+GGG - chr2.2:52117820-52117839 MS.gene004589:CDS 35.0%
! CTTTGGCAATAACCTTTTGT+GGG + chr2.2:52117384-52117403 None:intergenic 35.0%
! GAACTGAATTAGGCTTTTCA+AGG + chr2.2:52117277-52117296 None:intergenic 35.0%
! TTTGGCAATAACCTTTTGTG+GGG + chr2.2:52117383-52117402 None:intergenic 35.0%
! TTTGGGTTAATCAATTGGGT+GGG - chr2.2:52117933-52117952 MS.gene004589:CDS 35.0%
!! AAGTTGATGCCCTTAAGAAA+TGG - chr2.2:52117819-52117838 MS.gene004589:CDS 35.0%
!! ATTCCAAAAGTATTCGTCCA+AGG + chr2.2:52117752-52117771 None:intergenic 35.0%
!! TAGGTTCTTTAAAACCGACA+AGG - chr2.2:52117526-52117545 MS.gene004589:CDS 35.0%
!!! GTTTGTTTTCAGCTTTAAGG+AGG - chr2.2:52118123-52118142 MS.gene004589:CDS 35.0%
!!! TTTTGGGTTAATCAATTGGG+TGG - chr2.2:52117932-52117951 MS.gene004589:CDS 35.0%
AGACCAACCCCATTTCTTAA+GGG + chr2.2:52117831-52117850 None:intergenic 40.0%
CAAAGCTTTCAACAACAAGC+AGG - chr2.2:52117238-52117257 MS.gene004589:CDS 40.0%
CAAGTTACACATTCGGTAGA+TGG + chr2.2:52118226-52118245 None:intergenic 40.0%
CTCACCTTCTTAGAAAACCA+TGG - chr2.2:52117296-52117315 MS.gene004589:CDS 40.0%
GCAAACAATGGTATCCTTGT+CGG + chr2.2:52117543-52117562 None:intergenic 40.0%
GGTTGTGATGAAAGTAACCA+AGG + chr2.2:52117362-52117381 None:intergenic 40.0%
GTTGATGCCCTTAAGAAATG+GGG - chr2.2:52117821-52117840 MS.gene004589:CDS 40.0%
TAGACCAACCCCATTTCTTA+AGG + chr2.2:52117832-52117851 None:intergenic 40.0%
TGTTGTCGTGTGAAAAACCA+TGG + chr2.2:52117316-52117335 None:intergenic 40.0%
TTTCATCACAACCCCACAAA+AGG - chr2.2:52117369-52117388 MS.gene004589:CDS 40.0%
! AACAATTGTTCCAAGGGCAT+TGG - chr2.2:52118009-52118028 MS.gene004589:CDS 40.0%
! AGAGTTTAGGGTTTGATCCT+TGG - chr2.2:52117732-52117751 MS.gene004589:CDS 40.0%
! ATTCCTTGGCACTTGCTAAA+AGG - chr2.2:52117957-52117976 MS.gene004589:CDS 40.0%
! GATCCTTGGACGAATACTTT+TGG - chr2.2:52117746-52117765 MS.gene004589:CDS 40.0%
! GCCATTCACATTTGGTTCAA+AGG - chr2.2:52117682-52117701 MS.gene004589:CDS 40.0%
! TCCGCCACAAATGTAGTTTT+AGG - chr2.2:52117063-52117082 MS.gene004589:CDS 40.0%
!! AAAGCTTTGAGAGCAGATTG+AGG + chr2.2:52117227-52117246 None:intergenic 40.0%
ACACATTCGGTAGATGGCAT+TGG + chr2.2:52118220-52118239 None:intergenic 45.0%
ATGCCCTTAAGAAATGGGGT+TGG - chr2.2:52117825-52117844 MS.gene004589:CDS 45.0%
CAGTCAAGGCCATTCACATT+TGG - chr2.2:52117674-52117693 MS.gene004589:CDS 45.0%
TATTGCAGAACCAATGCCCT+TGG + chr2.2:52118022-52118041 None:intergenic 45.0%
TCAATTGGGTGGGAATTCCT+TGG - chr2.2:52117943-52117962 MS.gene004589:CDS 45.0%
! ATCGGGAACAACAAGTGTTG+AGG + chr2.2:52117104-52117123 None:intergenic 45.0%
! CAAGCATTTAGGATGCATCC+TGG - chr2.2:52117866-52117885 MS.gene004589:CDS 45.0%
!! ATCAGAGGAAGAAGCACCAT+TGG + chr2.2:52117423-52117442 None:intergenic 45.0%
!! TCGGGAACAACAAGTGTTGA+GGG + chr2.2:52117103-52117122 None:intergenic 45.0%
TTGCTAGCTCGCTTCAGTCA+AGG - chr2.2:52117660-52117679 MS.gene004589:CDS 50.0%
! TGGCCTTTTAGCAAGTGCCA+AGG + chr2.2:52117963-52117982 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 52117044 52118249 52117044 ID=MS.gene004589
chr2.2 mRNA 52117044 52118249 52117044 ID=MS.gene004589.t1;Parent=MS.gene004589
chr2.2 exon 52117044 52118249 52117044 ID=MS.gene004589.t1.exon1;Parent=MS.gene004589.t1
chr2.2 CDS 52117044 52118249 52117044 ID=cds.MS.gene004589.t1;Parent=MS.gene004589.t1
Gene Sequence

>MS.gene004589

ATGTTGAAGAAATTGTTTCTCCGCCACAAATGTAGTTTTAGGTCTCATCACAACAACCCTCAACACTTGTTGTTCCCGATTTCACTCATCAGATACTACAACACTACAATAACAACAACATCTGAATCAAACACACACCCATTCGCTGTATCATACCTTATCAACAATTTTGGTTTCTCACCTCAATCTGCTCTCAAAGCTTTCAACAACAAGCAGGTTCGTTTCAATACCCTTGAAAAGCCTAATTCAGTTCTCACCTTCTTAGAAAACCATGGTTTTTCACACGACAACATACGCATCATCATCAAAAAAGAACCTTGGTTACTTTCATCACAACCCCACAAAAGGTTATTGCCAAAGTTTCAATTTTTTCTATCCAATGGTGCTTCTTCCTCTGATATTGTTTCATCGTTAACTTCAAACCCTAGAATTTTACGAAGTAGCTTGGAGAAACAAATAATCCCTCTTTTTCAATTGTTAAATAGGTTCTTTAAAACCGACAAGGATACCATTGTTTGCTTAATTCGATGTTGGACTGCACTTGCTATTAATCCCTATCACCTTATTGTGTCTAATATCAATTTAATGTCTGATTTTGGAGTTTCTGATTCTGTCATTGCTAGCTCGCTTCAGTCAAGGCCATTCACATTTGGTTCAAAGGATTTGATTAAGTCATTGGAGGAAGTTAAGAGTTTAGGGTTTGATCCTTGGACGAATACTTTTGGAATTGCTTTGGCAGCTAAGAAAAGTTTGAGTAAAAAACTTTGGGATGAGAAAGTTGATGCCCTTAAGAAATGGGGTTGGTCTAATGAAGCTGTTTTTCAAGCATTTAGGATGCATCCTGGTTTCATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGTTTTTGGGTTAATCAATTGGGTGGGAATTCCTTGGCACTTGCTAAAAGGCCACATATTTTTGATTCAAGTTTGCAGAAAACAATTGTTCCAAGGGCATTGGTTCTGCAATATCTTGTGATGAAAGGTTTGAGGAAAAAGAATGCAAGCTTAGTTGAACCATTTCGTTATTCTGGGAAAATGTTCTTGAGCAAGTTTGTTTTCAGCTTTAAGGAGGAGTCTGATTATCTATTAAAGTTATATGACGAAAAAATGAAACTTGCATACACAAAAGAGAACAATGGCATGCCAATGCCATCTACCGAATGTGTAACTTGTTAA
Protein sequence

>MS.gene004589.t1

MLKKLFLRHKCSFRSHHNNPQHLLFPISLIRYYNTTITTTSESNTHPFAVSYLINNFGFSPQSALKAFNNKQVRFNTLEKPNSVLTFLENHGFSHDNIRIIIKKEPWLLSSQPHKRLLPKFQFFLSNGASSSDIVSSLTSNPRILRSSLEKQIIPLFQLLNRFFKTDKDTIVCLIRCWTALAINPYHLIVSNINLMSDFGVSDSVIASSLQSRPFTFGSKDLIKSLEEVKSLGFDPWTNTFGIALAAKKSLSKKLWDEKVDALKKWGWSNEAVFQAFRMHPGFMLTSIDKINLVMSFWVNQLGGNSLALAKRPHIFDSSLQKTIVPRALVLQYLVMKGLRKKNASLVEPFRYSGKMFLSKFVFSFKEESDYLLKLYDEKMKLAYTKENNGMPMPSTECVTC