AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene00324

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00324.t1 MTR_2g437080 95.526 380 17 0 1 380 1 380 0.0 745
MS.gene00324.t1 MTR_2g437060 73.834 386 89 2 6 379 9 394 0.0 564
MS.gene00324.t1 MTR_2g437030 66.753 388 119 2 1 379 79 465 1.39e-180 510
MS.gene00324.t1 MTR_2g436400 68.280 372 116 1 5 374 10 381 5.86e-180 505
MS.gene00324.t1 MTR_2g437020 65.455 385 121 4 3 382 5 382 2.02e-178 501
MS.gene00324.t1 MTR_2g436440 64.156 385 132 2 1 379 1 385 1.85e-175 494
MS.gene00324.t1 MTR_2g437040 62.533 379 142 0 1 379 1 379 1.86e-167 473
MS.gene00324.t1 MTR_2g437120 53.672 354 159 3 27 376 37 389 3.66e-129 377
MS.gene00324.t1 MTR_2g437160 52.909 361 168 1 22 380 45 405 2.18e-128 375
MS.gene00324.t1 MTR_2g437200 50.000 388 152 4 23 370 13 398 4.10e-125 366
MS.gene00324.t1 MTR_2g437960 52.663 338 157 1 42 376 55 392 6.73e-124 363
MS.gene00324.t1 MTR_2g437960 52.663 338 157 1 42 376 55 392 1.44e-123 363
MS.gene00324.t1 MTR_2g437170 51.955 358 163 3 32 380 43 400 1.75e-123 363
MS.gene00324.t1 MTR_2g437150 51.507 365 167 4 26 380 36 400 1.23e-122 360
MS.gene00324.t1 MTR_2g437990 51.657 362 166 3 27 380 32 392 1.31e-122 361
MS.gene00324.t1 MTR_2g437180 52.089 359 164 5 26 380 36 390 4.55e-121 356
MS.gene00324.t1 MTR_2g076320 49.862 363 172 3 27 380 32 393 1.45e-119 352
MS.gene00324.t1 MTR_2g437130 50.423 355 172 2 22 376 27 377 1.21e-118 349
MS.gene00324.t1 MTR_2g437260 47.170 371 189 4 8 374 3 370 1.73e-118 349
MS.gene00324.t1 MTR_4g119550 50.725 345 167 2 36 379 47 389 2.48e-118 349
MS.gene00324.t1 MTR_2g438010 49.198 374 172 4 24 380 41 413 5.73e-118 349
MS.gene00324.t1 MTR_2g438020 48.942 378 153 3 39 378 70 445 1.06e-117 351
MS.gene00324.t1 MTR_2g437240 46.944 409 172 6 14 380 3 408 4.49e-116 344
MS.gene00324.t1 MTR_2g437100 52.368 359 157 4 26 370 37 395 1.44e-114 340
MS.gene00324.t1 MTR_2g436460 45.600 375 188 3 11 371 5 377 1.33e-107 321
MS.gene00324.t1 MTR_2g437940 48.169 355 150 4 26 376 36 360 1.50e-103 310
MS.gene00324.t1 MTR_4g119580 44.315 343 187 2 30 370 32 372 1.65e-95 290
MS.gene00324.t1 MTR_2g019840 45.745 376 193 8 1 370 1 371 3.95e-92 281
MS.gene00324.t1 MTR_2g019810 47.289 332 169 4 41 370 32 359 6.36e-92 280
MS.gene00324.t1 MTR_4g120380 44.311 334 184 1 34 367 38 369 4.81e-91 278
MS.gene00324.t1 MTR_4g120380 44.444 315 173 1 34 348 38 350 3.81e-85 265
MS.gene00324.t1 MTR_2g437090 61.765 170 65 0 213 382 1 170 8.09e-75 230
MS.gene00324.t1 MTR_2g436370 59.893 187 73 1 5 189 9 195 8.62e-68 213
MS.gene00324.t1 MTR_2g437160 54.229 201 89 1 183 380 114 314 3.23e-67 216
MS.gene00324.t1 MTR_3g450500 31.977 344 210 5 41 370 39 372 8.80e-52 177
MS.gene00324.t1 MTR_4g119570 38.057 247 120 1 120 366 14 227 3.94e-49 166
MS.gene00324.t1 MTR_4g007490 29.459 370 243 6 5 368 11 368 3.85e-44 157
MS.gene00324.t1 MTR_8g080990 29.641 334 229 4 35 364 37 368 4.55e-35 132
MS.gene00324.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 2.13e-29 118
MS.gene00324.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 2.37e-29 117
MS.gene00324.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 2.66e-29 117
MS.gene00324.t1 MTR_4g049400 45.455 121 61 2 26 141 31 151 7.63e-25 101
MS.gene00324.t1 MTR_2g436380 72.131 61 17 0 213 273 1 61 9.17e-24 100
MS.gene00324.t1 MTR_3g085240 37.589 141 78 3 23 163 41 171 6.09e-20 88.6
MS.gene00324.t1 MTR_3g085240 64.151 53 19 0 272 324 176 228 3.44e-15 75.1
MS.gene00324.t1 MTR_8g012210 21.854 302 201 7 86 368 63 348 1.60e-12 68.6
MS.gene00324.t1 MTR_4g119615 52.000 50 24 0 312 361 26 75 8.64e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene00324.t1 AT5G07900 32.520 369 241 5 5 367 33 399 7.56e-63 207
MS.gene00324.t1 AT1G21150 30.792 341 228 4 31 365 10 348 1.40e-52 179
MS.gene00324.t1 AT1G21150 31.722 331 218 4 41 365 56 384 3.42e-52 179
MS.gene00324.t1 AT1G21150 31.722 331 218 4 41 365 120 448 1.36e-51 179
MS.gene00324.t1 AT1G61980 27.559 381 205 10 41 367 52 415 1.26e-34 132
MS.gene00324.t1 AT1G61980 27.559 381 205 10 41 367 52 415 1.26e-34 132
MS.gene00324.t1 AT1G61980 27.559 381 205 10 41 367 52 415 1.26e-34 132
MS.gene00324.t1 AT5G64950 27.451 357 234 8 31 368 26 376 2.33e-32 125
MS.gene00324.t1 AT1G61990 26.603 421 238 10 2 367 4 408 9.26e-32 124
MS.gene00324.t1 AT1G61990 26.603 421 238 10 2 367 4 408 9.26e-32 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G61970 27.762 353 208 8 42 353 53 399 1.04e-31 124
MS.gene00324.t1 AT1G62120 27.964 329 193 6 42 330 57 381 2.07e-29 118
MS.gene00324.t1 AT1G62085 27.295 414 212 13 2 329 4 414 2.78e-29 118
MS.gene00324.t1 AT1G62085 27.295 414 212 13 2 329 4 414 2.78e-29 118
MS.gene00324.t1 AT3G46950 31.716 268 171 6 22 285 32 291 4.83e-29 117
MS.gene00324.t1 AT5G23930 25.888 394 214 8 41 363 55 441 1.36e-26 110
MS.gene00324.t1 AT1G62110 28.571 245 170 3 42 283 53 295 3.64e-24 103
MS.gene00324.t1 AT1G62010 26.087 299 178 5 71 330 65 359 3.37e-23 100
MS.gene00324.t1 AT1G62150 23.035 369 209 7 35 330 52 418 1.29e-22 99.4
MS.gene00324.t1 AT1G61960 28.283 297 191 8 3 283 4 294 3.80e-22 97.8
MS.gene00324.t1 AT1G79220 24.662 296 182 8 79 369 119 378 7.67e-11 63.5

Find 80 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.083683 2.1:-56256687 MS.gene00324:CDS
TGAAGGTGGTGGATGAATTA+AGG 0.202555 2.1:-56256179 MS.gene00324:CDS
GAAAATGGTAATGGGGTTAT+TGG 0.222266 2.1:+56256756 None:intergenic
AAAGGGTTTGAGATTGAAAA+TGG 0.228978 2.1:+56256794 None:intergenic
CGACAAAATATCTGGTGTTT+TGG 0.229116 2.1:+56255918 None:intergenic
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2.1:-56255975 MS.gene00324:CDS
TTTCCTGCGACCCCTCTAAA+AGG 0.248958 2.1:-56256512 MS.gene00324:CDS
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2.1:-56255856 MS.gene00324:CDS
GTGGATGAATTAAGGGATTT+GGG 0.278480 2.1:-56256171 MS.gene00324:CDS
GGACCAACCCCACTTCTTAA+AGG 0.299701 2.1:+56256062 None:intergenic
GGTGGATGAATTAAGGGATT+TGG 0.320429 2.1:-56256172 MS.gene00324:CDS
TTTCTCTTTCCACAAGGCTT+TGG 0.324915 2.1:+56256092 None:intergenic
TGAGATTGAAAATGGATCTA+TGG 0.333993 2.1:+56256802 None:intergenic
AAAATGGTAATGGGGTTATT+GGG 0.341596 2.1:+56256757 None:intergenic
CGAGAACAGAATCAGGGTTT+TGG 0.341656 2.1:+56256604 None:intergenic
AATGAAGAGAAAATGGTAAT+GGG 0.355617 2.1:+56256748 None:intergenic
TATCGTTCCAATCTATGAAT+TGG 0.356154 2.1:-56256382 MS.gene00324:CDS
GTGCAGAAATGAAGAGAAAA+TGG 0.357192 2.1:+56256741 None:intergenic
CAAATATTTCTAGATTGCTT+AGG 0.360863 2.1:-56256236 MS.gene00324:CDS
GAAGGTGGTGGATGAATTAA+GGG 0.364635 2.1:-56256178 MS.gene00324:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.1:-56255956 MS.gene00324:CDS
AAGAAATCGAGAACAGAATC+AGG 0.368524 2.1:+56256597 None:intergenic
TGTTCGTGGTGTATGCAAAA+TGG 0.372913 2.1:+56255707 None:intergenic
AAACTTAACGACAAAATATC+TGG 0.378028 2.1:+56255910 None:intergenic
GGGTTTGGTGAGAAAGTAAA+GGG 0.409377 2.1:+56256777 None:intergenic
CTATAAACCAATTCATAGAT+TGG 0.415060 2.1:+56256375 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.1:+56256461 None:intergenic
GAAAGTAGCCACGGTGCCTT+GGG 0.421775 2.1:+56256531 None:intergenic
GGAAAGTAGCCACGGTGCCT+TGG 0.424368 2.1:+56256530 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.1:-56255957 MS.gene00324:CDS
GGTAATGGGGTTATTGGGTT+TGG 0.430082 2.1:+56256762 None:intergenic
AAATATTTCTAGATTGCTTA+GGG 0.430507 2.1:-56256235 MS.gene00324:CDS
ATTACATTGTTGCTTCAGAA+TGG 0.447044 2.1:-56256270 MS.gene00324:CDS
AATTGCACAACAGTGGCTCT+TGG 0.457636 2.1:+56255871 None:intergenic
GGGAATGATACGACGTAGTT+GGG 0.467688 2.1:+56256551 None:intergenic
AGATGGTCACTAAATAAAAC+AGG 0.477887 2.1:+56256306 None:intergenic
AAATGAAGAGAAAATGGTAA+TGG 0.484200 2.1:+56256747 None:intergenic
TGGGTTTGGTGAGAAAGTAA+AGG 0.484654 2.1:+56256776 None:intergenic
TGGGAATGATACGACGTAGT+TGG 0.489187 2.1:+56256550 None:intergenic
AACTTCTCAGGCATTAAGAA+TGG 0.491818 2.1:+56255802 None:intergenic
CTCGATTTCTTTAGAAACCA+TGG 0.495225 2.1:-56256585 MS.gene00324:CDS
GTATCAAGAAATAACTTCTC+AGG 0.495468 2.1:+56255790 None:intergenic
TTTATTTAGTGACCATCTTG+TGG 0.528406 2.1:-56256301 MS.gene00324:CDS
CAAAGCTTAGAAGCAAATTG+TGG 0.530033 2.1:+56256657 None:intergenic
ATGCCTTTAAGAAGTGGGGT+TGG 0.530413 2.1:-56256065 MS.gene00324:CDS
TCAGTTGGGTTGGGATGCTA+AGG 0.533835 2.1:-56255947 MS.gene00324:CDS
CAAGCTTACAGACAAGATCC+TGG 0.534656 2.1:+56256413 None:intergenic
AAGCTTACAGACAAGATCCT+GGG 0.540938 2.1:+56256414 None:intergenic
AGTTGATGCCTTTAAGAAGT+GGG 0.541443 2.1:-56256070 MS.gene00324:CDS
TTCCTGCGACCCCTCTAAAA+GGG 0.542437 2.1:-56256511 MS.gene00324:CDS
CTTGAAACGGAGCTTGTAAG+TGG 0.546088 2.1:+56256632 None:intergenic
CAACTTTAAAAGATTAGAAG+CGG 0.547155 2.1:+56255744 None:intergenic
AAGTTGATGCCTTTAAGAAG+TGG 0.551383 2.1:-56256071 MS.gene00324:CDS
AAAGCTTAGAAGCAAATTGT+GGG 0.556435 2.1:+56256658 None:intergenic
CCCAAAATTGTTGATGAGGT+AGG 0.556895 2.1:+56256686 None:intergenic
ACTTTATGAGTAAGAACCCC+AGG 0.563877 2.1:-56256431 MS.gene00324:CDS
CAAACCCAAAATTGTTGATG+AGG 0.576353 2.1:+56256682 None:intergenic
TAGATTCTTGCAATCGCACA+AGG 0.582615 2.1:-56256355 MS.gene00324:CDS
CATCAACAACCAAAGCCTTG+TGG 0.591454 2.1:-56256101 MS.gene00324:CDS
CAATCTAGAAATATTTGAGT+CGG 0.591851 2.1:+56256242 None:intergenic
ATGAAGAGAAAATGGTAATG+GGG 0.595101 2.1:+56256749 None:intergenic
TGCTTCAAGAACATCTTCAT+CGG 0.599346 2.1:+56256041 None:intergenic
ATCAACTTTCTCTTTCCACA+AGG 0.604525 2.1:+56256086 None:intergenic
TGGATGAATTAAGGGATTTG+GGG 0.606794 2.1:-56256170 MS.gene00324:CDS
TAATGTTATGTGCCACAAGA+TGG 0.610046 2.1:+56256289 None:intergenic
GAGGTAGGAAACTGCAAATG+AGG 0.610228 2.1:+56256701 None:intergenic
CACATGTGTGCAATGTCCTA+CGG 0.613554 2.1:+56256208 None:intergenic
GTTGATGCCTTTAAGAAGTG+GGG 0.614341 2.1:-56256069 MS.gene00324:CDS
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2.1:+56256568 None:intergenic
AGAAATCGAGAACAGAATCA+GGG 0.621735 2.1:+56256598 None:intergenic
GGGTCGCAGGAAAGTAGCCA+CGG 0.625027 2.1:+56256522 None:intergenic
TGAGGTTGAATCTGAAGTGT+TGG 0.626308 2.1:+56256719 None:intergenic
CATAAAGTTAACCATGTCAG+AGG 0.632724 2.1:+56256446 None:intergenic
AGGTGCTTCTACCTCTGACA+TGG 0.645076 2.1:-56256457 MS.gene00324:CDS
GAGAAGAAATTGCACAACAG+TGG 0.646434 2.1:+56255864 None:intergenic
ATGGATGTTAACATACAATG+AGG 0.655070 2.1:+56256006 None:intergenic
ACTACGTCGTATCATTCCCA+AGG 0.661929 2.1:-56256547 MS.gene00324:CDS
AGCTTACAGACAAGATCCTG+GGG 0.666533 2.1:+56256415 None:intergenic
GATTGCTTAGGGAACACCGT+AGG 0.718101 2.1:-56256224 MS.gene00324:CDS
GTATCATTCCCAAGGCACCG+TGG 0.787154 2.1:-56256539 MS.gene00324:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG + chr2.1:56256525-56256544 None:intergenic 15.0%
!!! AAATATTTCTAGATTGCTTA+GGG - chr2.1:56256274-56256293 MS.gene00324:CDS 20.0%
! AAACTTAACGACAAAATATC+TGG + chr2.1:56256602-56256621 None:intergenic 25.0%
! AAATGAAGAGAAAATGGTAA+TGG + chr2.1:56255765-56255784 None:intergenic 25.0%
! AATGAAGAGAAAATGGTAAT+GGG + chr2.1:56255764-56255783 None:intergenic 25.0%
! CAATCTAGAAATATTTGAGT+CGG + chr2.1:56256270-56256289 None:intergenic 25.0%
! CTATAAACCAATTCATAGAT+TGG + chr2.1:56256137-56256156 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.1:56256029-56256048 None:intergenic 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.1:56256365-56256384 MS.gene00324:CDS 25.0%
!! AGATATTTTGTCGTTAAGTT+TGG - chr2.1:56256601-56256620 MS.gene00324:CDS 25.0%
!! CAACTTTAAAAGATTAGAAG+CGG + chr2.1:56256768-56256787 None:intergenic 25.0%
!! TAATTTAGTGATGAGCTTTT+GGG - chr2.1:56256535-56256554 MS.gene00324:CDS 25.0%
!! TTAATTTAGTGATGAGCTTT+TGG - chr2.1:56256534-56256553 MS.gene00324:CDS 25.0%
!!! CAAATATTTCTAGATTGCTT+AGG - chr2.1:56256273-56256292 MS.gene00324:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.1:56256032-56256051 MS.gene00324:CDS 25.0%
AAAGCTTAGAAGCAAATTGT+GGG + chr2.1:56255854-56255873 None:intergenic 30.0%
AGATGGTCACTAAATAAAAC+AGG + chr2.1:56256206-56256225 None:intergenic 30.0%
ATGGATGTTAACATACAATG+AGG + chr2.1:56256506-56256525 None:intergenic 30.0%
GTATCAAGAAATAACTTCTC+AGG + chr2.1:56256722-56256741 None:intergenic 30.0%
GTGCAATTTCTTCTCAATAA+TGG - chr2.1:56256653-56256672 MS.gene00324:CDS 30.0%
TATCGTTCCAATCTATGAAT+TGG - chr2.1:56256127-56256146 MS.gene00324:CDS 30.0%
TGAGATTGAAAATGGATCTA+TGG + chr2.1:56255710-56255729 None:intergenic 30.0%
TGTCGTTAAGTTTGGAAAAA+AGG - chr2.1:56256609-56256628 MS.gene00324:CDS 30.0%
TTTATTTAGTGACCATCTTG+TGG - chr2.1:56256208-56256227 MS.gene00324:CDS 30.0%
! AAAGGGTTTGAGATTGAAAA+TGG + chr2.1:56255718-56255737 None:intergenic 30.0%
! ATGAAGAGAAAATGGTAATG+GGG + chr2.1:56255763-56255782 None:intergenic 30.0%
! ATTACATTGTTGCTTCAGAA+TGG - chr2.1:56256239-56256258 MS.gene00324:CDS 30.0%
! TTTCGTGTAATTTTTGTTCG+TGG + chr2.1:56256819-56256838 None:intergenic 30.0%
!! AAAATGGTAATGGGGTTATT+GGG + chr2.1:56255755-56255774 None:intergenic 30.0%
!!! ACACCAAAAGTTGTTTTTGA+AGG + chr2.1:56256371-56256390 None:intergenic 30.0%
AACTTCTCAGGCATTAAGAA+TGG + chr2.1:56256710-56256729 None:intergenic 35.0%
AAGAAATCGAGAACAGAATC+AGG + chr2.1:56255915-56255934 None:intergenic 35.0%
AGAAATCGAGAACAGAATCA+GGG + chr2.1:56255914-56255933 None:intergenic 35.0%
ATCAACTTTCTCTTTCCACA+AGG + chr2.1:56256426-56256445 None:intergenic 35.0%
CAAACCCAAAATTGTTGATG+AGG + chr2.1:56255830-56255849 None:intergenic 35.0%
CAAAGCTTAGAAGCAAATTG+TGG + chr2.1:56255855-56255874 None:intergenic 35.0%
CATAAAGTTAACCATGTCAG+AGG + chr2.1:56256066-56256085 None:intergenic 35.0%
GTGCAGAAATGAAGAGAAAA+TGG + chr2.1:56255771-56255790 None:intergenic 35.0%
GTGGATGAATTAAGGGATTT+GGG - chr2.1:56256338-56256357 MS.gene00324:CDS 35.0%
TAATGTTATGTGCCACAAGA+TGG + chr2.1:56256223-56256242 None:intergenic 35.0%
TGCTTCAAGAACATCTTCAT+CGG + chr2.1:56256471-56256490 None:intergenic 35.0%
TGGATGAATTAAGGGATTTG+GGG - chr2.1:56256339-56256358 MS.gene00324:CDS 35.0%
! AGTTGATGCCTTTAAGAAGT+GGG - chr2.1:56256439-56256458 MS.gene00324:CDS 35.0%
! CCTACCTCATCAACAATTTT+GGG - chr2.1:56255823-56255842 MS.gene00324:CDS 35.0%
! CGACAAAATATCTGGTGTTT+TGG + chr2.1:56256594-56256613 None:intergenic 35.0%
! CTCGATTTCTTTAGAAACCA+TGG - chr2.1:56255924-56255943 MS.gene00324:CDS 35.0%
! TCCTACCTCATCAACAATTT+TGG - chr2.1:56255822-56255841 MS.gene00324:CDS 35.0%
!! AAGTTGATGCCTTTAAGAAG+TGG - chr2.1:56256438-56256457 MS.gene00324:CDS 35.0%
!! GAAAATGGTAATGGGGTTAT+TGG + chr2.1:56255756-56255775 None:intergenic 35.0%
AAGCTTACAGACAAGATCCT+GGG + chr2.1:56256098-56256117 None:intergenic 40.0%
ACTTTATGAGTAAGAACCCC+AGG - chr2.1:56256078-56256097 MS.gene00324:CDS 40.0%
GAGAAGAAATTGCACAACAG+TGG + chr2.1:56256648-56256667 None:intergenic 40.0%
GGTGGATGAATTAAGGGATT+TGG - chr2.1:56256337-56256356 MS.gene00324:CDS 40.0%
TAGATTCTTGCAATCGCACA+AGG - chr2.1:56256154-56256173 MS.gene00324:CDS 40.0%
TGTTCGTGGTGTATGCAAAA+TGG + chr2.1:56256805-56256824 None:intergenic 40.0%
TTTCTCTTTCCACAAGGCTT+TGG + chr2.1:56256420-56256439 None:intergenic 40.0%
! CCCAAAATTGTTGATGAGGT+AGG + chr2.1:56255826-56255845 None:intergenic 40.0%
! GCACACATGTGACATTTTGA+AGG - chr2.1:56256313-56256332 MS.gene00324:CDS 40.0%
! GTTGATGCCTTTAAGAAGTG+GGG - chr2.1:56256440-56256459 MS.gene00324:CDS 40.0%
! TGAGGTTGAATCTGAAGTGT+TGG + chr2.1:56255793-56255812 None:intergenic 40.0%
! TTTGGCAACACCCTTTTAGA+GGG + chr2.1:56256011-56256030 None:intergenic 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.1:56256548-56256567 MS.gene00324:CDS 40.0%
!! ATGTGACATTTTGAAGGTGG+TGG - chr2.1:56256319-56256338 MS.gene00324:CDS 40.0%
!! CACATGTGACATTTTGAAGG+TGG - chr2.1:56256316-56256335 MS.gene00324:CDS 40.0%
!! GAAGGTGGTGGATGAATTAA+GGG - chr2.1:56256331-56256350 MS.gene00324:CDS 40.0%
!! GGGTTTGGTGAGAAAGTAAA+GGG + chr2.1:56255735-56255754 None:intergenic 40.0%
!! GGTTTTGGTCAGTCTTGAAA+CGG + chr2.1:56255893-56255912 None:intergenic 40.0%
!! TGAAGGTGGTGGATGAATTA+AGG - chr2.1:56256330-56256349 MS.gene00324:CDS 40.0%
!! TGGGTTTGGTGAGAAAGTAA+AGG + chr2.1:56255736-56255755 None:intergenic 40.0%
AATTGCACAACAGTGGCTCT+TGG + chr2.1:56256641-56256660 None:intergenic 45.0%
ACTACGTCGTATCATTCCCA+AGG - chr2.1:56255962-56255981 MS.gene00324:CDS 45.0%
AGCTTACAGACAAGATCCTG+GGG + chr2.1:56256097-56256116 None:intergenic 45.0%
CAAGCTTACAGACAAGATCC+TGG + chr2.1:56256099-56256118 None:intergenic 45.0%
CACATGTGTGCAATGTCCTA+CGG + chr2.1:56256304-56256323 None:intergenic 45.0%
CATCAACAACCAAAGCCTTG+TGG - chr2.1:56256408-56256427 MS.gene00324:CDS 45.0%
CTTGAAACGGAGCTTGTAAG+TGG + chr2.1:56255880-56255899 None:intergenic 45.0%
GAGGTAGGAAACTGCAAATG+AGG + chr2.1:56255811-56255830 None:intergenic 45.0%
GGGAATGATACGACGTAGTT+GGG + chr2.1:56255961-56255980 None:intergenic 45.0%
GTTGGGAGTTAGAGAAACCA+TGG + chr2.1:56255944-56255963 None:intergenic 45.0%
TGGGAATGATACGACGTAGT+TGG + chr2.1:56255962-56255981 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.1:56256553-56256572 MS.gene00324:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.1:56256552-56256571 MS.gene00324:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG - chr2.1:56256444-56256463 MS.gene00324:CDS 45.0%
! CGAGAACAGAATCAGGGTTT+TGG + chr2.1:56255908-56255927 None:intergenic 45.0%
! CTTTGGCAACACCCTTTTAG+AGG + chr2.1:56256012-56256031 None:intergenic 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.1:56256547-56256566 MS.gene00324:CDS 45.0%
!! GGTAATGGGGTTATTGGGTT+TGG + chr2.1:56255750-56255769 None:intergenic 45.0%
!! TTGGCAACACCCTTTTAGAG+GGG + chr2.1:56256010-56256029 None:intergenic 45.0%
AGGTGCTTCTACCTCTGACA+TGG - chr2.1:56256052-56256071 MS.gene00324:CDS 50.0%
GATTGCTTAGGGAACACCGT+AGG - chr2.1:56256285-56256304 MS.gene00324:CDS 50.0%
GGACCAACCCCACTTCTTAA+AGG + chr2.1:56256450-56256469 None:intergenic 50.0%
TTTCCTGCGACCCCTCTAAA+AGG - chr2.1:56255997-56256016 MS.gene00324:CDS 50.0%
! TTCCTGCGACCCCTCTAAAA+GGG - chr2.1:56255998-56256017 MS.gene00324:CDS 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.1:56256051-56256070 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+AGG - chr2.1:56256562-56256581 MS.gene00324:CDS 50.0%
GAAAGTAGCCACGGTGCCTT+GGG + chr2.1:56255981-56256000 None:intergenic 55.0%
!! GTATCATTCCCAAGGCACCG+TGG - chr2.1:56255970-56255989 MS.gene00324:CDS 55.0%
GGAAAGTAGCCACGGTGCCT+TGG + chr2.1:56255982-56256001 None:intergenic 60.0%
GGGTCGCAGGAAAGTAGCCA+CGG + chr2.1:56255990-56256009 None:intergenic 60.0%
!! CACCCTTTTAGAGGGGTCGC+AGG + chr2.1:56256003-56256022 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56255690 56256841 56255690 ID=MS.gene00324
chr2.1 mRNA 56255690 56256841 56255690 ID=MS.gene00324.t1;Parent=MS.gene00324
chr2.1 exon 56255690 56256841 56255690 ID=MS.gene00324.t1.exon1;Parent=MS.gene00324.t1
chr2.1 CDS 56255690 56256841 56255690 ID=cds.MS.gene00324.t1;Parent=MS.gene00324.t1
Gene Sequence

>MS.gene00324

ATGTTGATTATTCATCGCCATAGATCCATTTTCAATCTCAAACCCTTTACTTTCTCACCAAACCCAATAACCCCATTACCATTTTCTCTTCATTTCTGCACCAACACTTCAGATTCAACCTCATTTGCAGTTTCCTACCTCATCAACAATTTTGGGTTTGACCCACAATTTGCTTCTAAGCTTTGCTCCACTTACAAGCTCCGTTTCAAGACTGACCAAAACCCTGATTCTGTTCTCGATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTATCATTCCCAAGGCACCGTGGCTACTTTCCTGCGACCCCTCTAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATGGTTAACTTTATGAGTAAGAACCCCAGGATCTTGTCTGTAAGCTTGAAGAATCGTATCGTTCCAATCTATGAATTGGTTTATAGATTCTTGCAATCGCACAAGGACACTATTGCTTGTGTAATTAGCAATCCTGTTTTATTTAGTGACCATCTTGTGGCACATAACATTACATTGTTGCTTCAGAATGGACTCTCCGACTCAAATATTTCTAGATTGCTTAGGGAACACCGTAGGACATTGCACACATGTGACATTTTGAAGGTGGTGGATGAATTAAGGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCTTTGATAGCGAAATCATCAACAACCAAAGCCTTGTGGAAAGAGAAAGTTGATGCCTTTAAGAAGTGGGGTTGGTCCGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTAAGGACATTGCCAAAACACCAGATATTTTGTCGTTAAGTTTGGAAAAAAGGATCATTCCAAGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAATAAGAATTCAAGCTTAATTTATCCATTCTTAATGCCTGAGAAGTTATTTCTTGATACGTTTGTAAAACGTTTTGAGAATGCCGCTTCTAATCTTTTAAAGTTGTATGAAGAAAAACTCCATTTTGCATACACCACGAACAAAAATTACACGAAATGA
Protein sequence

>MS.gene00324.t1

MLIIHRHRSIFNLKPFTFSPNPITPLPFSLHFCTNTSDSTSFAVSYLINNFGFDPQFASKLCSTYKLRFKTDQNPDSVLDFFRNHGFSNSQLRRIIPKAPWLLSCDPSKRVLPKFQFFLSKGASTSDMVNFMSKNPRILSVSLKNRIVPIYELVYRFLQSHKDTIACVISNPVLFSDHLVAHNITLLLQNGLSDSNISRLLREHRRTLHTCDILKVVDELRDLGFNPSKTTFGVALIAKSSTTKALWKEKVDAFKKWGWSDEDVLEAFKKQPHCMLTSIDKINLVMSFWVNQLGWDAKDIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRNKNSSLIYPFLMPEKLFLDTFVKRFENAASNLLKLYEEKLHFAYTTNKNYTK