AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073373

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073373.t1 MTR_2g437080 95.026 382 19 0 1 382 1 382 0.0 748
MS.gene073373.t1 MTR_2g437060 74.352 386 87 2 6 379 9 394 0.0 568
MS.gene073373.t1 MTR_2g437030 66.923 390 119 2 1 381 79 467 0.0 516
MS.gene073373.t1 MTR_2g437020 65.974 385 119 4 3 382 5 382 0.0 507
MS.gene073373.t1 MTR_2g436400 68.548 372 115 1 5 374 10 381 1.58e-180 506
MS.gene073373.t1 MTR_2g436440 64.156 385 132 2 1 379 1 385 2.83e-175 493
MS.gene073373.t1 MTR_2g437040 63.325 379 139 0 1 379 1 379 4.73e-169 477
MS.gene073373.t1 MTR_2g437160 53.186 361 167 1 22 380 45 405 1.53e-129 378
MS.gene073373.t1 MTR_2g437120 52.825 354 162 3 27 376 37 389 2.50e-127 373
MS.gene073373.t1 MTR_2g437200 50.000 388 152 4 23 370 13 398 2.99e-125 367
MS.gene073373.t1 MTR_2g437960 52.663 338 157 1 42 376 55 392 1.65e-124 365
MS.gene073373.t1 MTR_2g437170 52.235 358 162 3 32 380 43 400 3.78e-124 364
MS.gene073373.t1 MTR_2g437960 52.663 338 157 1 42 376 55 392 3.86e-124 365
MS.gene073373.t1 MTR_2g437150 51.507 365 167 4 26 380 36 400 3.52e-123 362
MS.gene073373.t1 MTR_2g437990 51.515 363 167 3 26 380 31 392 6.45e-123 362
MS.gene073373.t1 MTR_2g437180 52.089 359 164 5 26 380 36 390 9.93e-122 358
MS.gene073373.t1 MTR_2g437260 47.439 371 188 4 8 374 3 370 6.54e-120 353
MS.gene073373.t1 MTR_2g076320 49.725 364 173 3 26 380 31 393 1.82e-119 352
MS.gene073373.t1 MTR_4g119550 51.014 345 166 2 36 379 47 389 4.11e-119 351
MS.gene073373.t1 MTR_2g438010 49.465 374 171 4 24 380 41 413 4.36e-119 352
MS.gene073373.t1 MTR_2g437130 50.423 355 172 2 22 376 27 377 5.00e-119 350
MS.gene073373.t1 MTR_2g437240 47.188 409 171 6 14 380 3 408 3.49e-117 347
MS.gene073373.t1 MTR_2g438020 48.148 378 156 3 39 378 70 445 3.99e-116 347
MS.gene073373.t1 MTR_2g437100 51.344 372 160 5 26 383 37 401 9.13e-116 343
MS.gene073373.t1 MTR_2g436460 45.213 376 190 3 12 373 6 379 2.10e-107 320
MS.gene073373.t1 MTR_2g437940 47.887 355 151 4 26 376 36 360 1.93e-103 310
MS.gene073373.t1 MTR_4g119580 44.575 341 185 2 30 368 32 370 4.70e-96 291
MS.gene073373.t1 MTR_2g019840 45.745 376 193 8 1 370 1 371 8.71e-92 281
MS.gene073373.t1 MTR_4g120380 44.179 335 185 1 34 368 38 370 1.61e-91 280
MS.gene073373.t1 MTR_2g019810 46.988 332 170 4 41 370 32 359 5.49e-91 278
MS.gene073373.t1 MTR_4g120380 44.444 315 173 1 34 348 38 350 1.51e-85 266
MS.gene073373.t1 MTR_2g437090 62.941 170 63 0 213 382 1 170 3.35e-77 236
MS.gene073373.t1 MTR_2g437160 48.571 245 109 2 153 380 70 314 1.93e-68 219
MS.gene073373.t1 MTR_2g436370 59.358 187 74 1 5 189 9 195 5.47e-67 211
MS.gene073373.t1 MTR_3g450500 31.977 344 210 5 41 370 39 372 8.80e-52 177
MS.gene073373.t1 MTR_4g119570 38.462 247 119 1 120 366 14 227 8.86e-50 167
MS.gene073373.t1 MTR_4g007490 29.459 370 243 6 5 368 11 368 5.49e-44 156
MS.gene073373.t1 MTR_8g080990 29.641 334 229 4 35 364 37 368 2.46e-35 133
MS.gene073373.t1 MTR_8g081000 27.726 321 213 4 57 368 75 385 7.05e-30 119
MS.gene073373.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 8.10e-30 119
MS.gene073373.t1 MTR_8g081000 27.653 311 210 3 67 368 81 385 8.11e-30 119
MS.gene073373.t1 MTR_4g049400 45.455 121 61 2 26 141 31 151 2.97e-25 102
MS.gene073373.t1 MTR_2g436380 72.131 61 17 0 213 273 1 61 9.45e-24 100
MS.gene073373.t1 MTR_3g085240 39.007 141 76 3 23 163 41 171 9.42e-21 90.9
MS.gene073373.t1 MTR_3g085240 64.151 53 19 0 272 324 176 228 3.37e-15 75.1
MS.gene073373.t1 MTR_8g012210 21.523 302 202 7 86 368 63 348 2.12e-12 68.2
MS.gene073373.t1 MTR_4g119615 52.000 50 24 0 312 361 26 75 8.48e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073373.t1 AT5G07900 32.791 369 240 5 5 367 33 399 2.58e-63 208
MS.gene073373.t1 AT1G21150 31.085 341 227 4 31 365 10 348 1.28e-52 179
MS.gene073373.t1 AT1G21150 32.024 331 217 4 41 365 56 384 1.36e-52 179
MS.gene073373.t1 AT1G21150 32.024 331 217 4 41 365 120 448 7.79e-52 179
MS.gene073373.t1 AT1G61980 27.733 375 212 9 41 367 52 415 3.58e-35 134
MS.gene073373.t1 AT1G61980 27.733 375 212 9 41 367 52 415 3.58e-35 134
MS.gene073373.t1 AT1G61980 27.733 375 212 9 41 367 52 415 3.58e-35 134
MS.gene073373.t1 AT5G64950 27.451 357 234 8 31 368 26 376 1.83e-32 126
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61970 27.762 353 208 8 42 353 53 399 3.67e-32 125
MS.gene073373.t1 AT1G61990 26.366 421 239 10 2 367 4 408 1.19e-31 124
MS.gene073373.t1 AT1G61990 26.366 421 239 10 2 367 4 408 1.19e-31 124
MS.gene073373.t1 AT3G46950 31.716 268 171 6 22 285 32 291 8.59e-30 119
MS.gene073373.t1 AT1G62120 26.816 358 217 7 42 358 57 410 2.28e-29 118
MS.gene073373.t1 AT1G62085 27.053 414 213 13 2 329 4 414 4.54e-29 117
MS.gene073373.t1 AT1G62085 27.053 414 213 13 2 329 4 414 4.54e-29 117
MS.gene073373.t1 AT5G23930 25.635 394 215 8 41 363 55 441 2.03e-26 110
MS.gene073373.t1 AT1G62110 28.163 245 171 3 42 283 53 295 3.00e-24 103
MS.gene073373.t1 AT1G62150 23.035 369 209 7 35 330 52 418 6.16e-23 100
MS.gene073373.t1 AT1G62010 25.418 299 180 5 71 330 65 359 2.39e-22 98.2
MS.gene073373.t1 AT1G61960 27.946 297 192 8 3 283 4 294 3.36e-22 98.2
MS.gene073373.t1 AT1G79220 23.779 307 193 8 79 380 119 389 5.65e-11 63.9

Find 81 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCCTACCTCATCAACAATTT+TGG 0.083683 2.4:-54616675 MS.gene073373:CDS
TGAAGGTGGTGGATGAATTA+AGG 0.202555 2.4:-54616167 MS.gene073373:CDS
AAAGGGTTTGAGATTGAAAA+TGG 0.228978 2.4:+54616782 None:intergenic
CGACAAAATATCTGGTGTTT+TGG 0.229116 2.4:+54615906 None:intergenic
GAAAAGGGTAATGGGGTTAT+TGG 0.230774 2.4:+54616744 None:intergenic
CGACCAACCCCACTTCTTAA+AGG 0.236453 2.4:+54616050 None:intergenic
TTAATTTAGTGATGAGCTTT+TGG 0.242008 2.4:-54615963 MS.gene073373:CDS
TTTCCTGCGACCCCTCTAAA+AGG 0.248958 2.4:-54616500 MS.gene073373:CDS
GTGCAATTTCTTCTCAATAA+TGG 0.259750 2.4:-54615844 MS.gene073373:CDS
GTGGATGAATTAAGGGATTT+GGG 0.278480 2.4:-54616159 MS.gene073373:CDS
TGAGAACTGAATCAGGGTTT+TGG 0.309396 2.4:+54616592 None:intergenic
AAAAGGGTAATGGGGTTATT+GGG 0.314656 2.4:+54616745 None:intergenic
GGTGGATGAATTAAGGGATT+TGG 0.320429 2.4:-54616160 MS.gene073373:CDS
TTTCTCTTTCCACAAGGCTT+TGG 0.324915 2.4:+54616080 None:intergenic
AAGAAATTGAGAACTGAATC+AGG 0.327189 2.4:+54616585 None:intergenic
TGAGATTGAAAATGGATCTA+TGG 0.333993 2.4:+54616790 None:intergenic
TATCGTTCCAATCTATGAAT+TGG 0.356154 2.4:-54616370 MS.gene073373:CDS
CAAATATTTCTAGATTGCTT+AGG 0.360863 2.4:-54616224 MS.gene073373:CDS
GAAGGTGGTGGATGAATTAA+GGG 0.364635 2.4:-54616166 MS.gene073373:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.4:-54615944 MS.gene073373:CDS
AAACTTAACGACAAAATATC+TGG 0.378028 2.4:+54615898 None:intergenic
AATGAAGAGAAAAGGGTAAT+GGG 0.382645 2.4:+54616736 None:intergenic
GGTAATGGGGTTATTGGGTT+TGG 0.408399 2.4:+54616750 None:intergenic
GGGTTTGGTGAGAAAGTAAA+GGG 0.409377 2.4:+54616765 None:intergenic
CTATAAACCAATTCATAGAT+TGG 0.415060 2.4:+54616363 None:intergenic
GTCAGAGGTAGAAGCACCTT+TGG 0.417863 2.4:+54616449 None:intergenic
GAAAGTAGCCACGGTGCCTT+GGG 0.421775 2.4:+54616519 None:intergenic
GGAAAGTAGCCACGGTGCCT+TGG 0.424368 2.4:+54616518 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.429067 2.4:-54615945 MS.gene073373:CDS
AAATATTTCTAGATTGCTTA+GGG 0.430507 2.4:-54616223 MS.gene073373:CDS
ATTACATTGTTGCTTCAGAA+TGG 0.447044 2.4:-54616258 MS.gene073373:CDS
GGGAATGACACGACGTAGTT+GGG 0.448752 2.4:+54616539 None:intergenic
AATTGCACAACAGTGGCTCT+TGG 0.457636 2.4:+54615859 None:intergenic
CTCAATTTCTTTAGAAACCA+TGG 0.466986 2.4:-54616573 MS.gene073373:CDS
AGATGGTCACTAAATAAAAC+AGG 0.477887 2.4:+54616294 None:intergenic
GTGCAGAAATGAAGAGAAAA+GGG 0.479138 2.4:+54616729 None:intergenic
TGGGTTTGGTGAGAAAGTAA+AGG 0.484654 2.4:+54616764 None:intergenic
GGTGCAGAAATGAAGAGAAA+AGG 0.490198 2.4:+54616728 None:intergenic
AACTTCTCAGGCATTAAGAA+TGG 0.491818 2.4:+54615790 None:intergenic
AAATGAAGAGAAAAGGGTAA+TGG 0.494135 2.4:+54616735 None:intergenic
GTATCAAGAAATAACTTCTC+AGG 0.495468 2.4:+54615778 None:intergenic
TGGGAATGACACGACGTAGT+TGG 0.498057 2.4:+54616538 None:intergenic
TTTATTTAGTGACCATCTTG+TGG 0.528406 2.4:-54616289 MS.gene073373:CDS
CAAAGCTTAGAAGCAAATTG+TGG 0.530033 2.4:+54616645 None:intergenic
TCAGTTGGGTTGGGATGCTA+AGG 0.533835 2.4:-54615935 MS.gene073373:CDS
CAAGCTTACAGACAAGATCC+TGG 0.534656 2.4:+54616401 None:intergenic
AAGCTTACAGACAAGATCCT+GGG 0.540938 2.4:+54616402 None:intergenic
AGTTGATGCCTTTAAGAAGT+GGG 0.541443 2.4:-54616058 MS.gene073373:CDS
TTCCTGCGACCCCTCTAAAA+GGG 0.542437 2.4:-54616499 MS.gene073373:CDS
CTTGAAACGGAGCTTGTAAG+TGG 0.546088 2.4:+54616620 None:intergenic
CAACTTTAAAAGATTAGAAG+CGG 0.547155 2.4:+54615732 None:intergenic
AAGTTGATGCCTTTAAGAAG+TGG 0.551383 2.4:-54616059 MS.gene073373:CDS
AAAGCTTAGAAGCAAATTGT+GGG 0.556435 2.4:+54616646 None:intergenic
CCCAAAATTGTTGATGAGGT+AGG 0.556895 2.4:+54616674 None:intergenic
CTTTAAGAAGTGGGGTTGGT+CGG 0.561255 2.4:-54616049 MS.gene073373:CDS
ACTTTATGAGTAAGAACCCC+AGG 0.563877 2.4:-54616419 MS.gene073373:CDS
ATGCCTTTAAGAAGTGGGGT+TGG 0.572339 2.4:-54616053 MS.gene073373:CDS
CAAACCCAAAATTGTTGATG+AGG 0.576353 2.4:+54616670 None:intergenic
TAGATTCTTGCAATCGCACA+AGG 0.582615 2.4:-54616343 MS.gene073373:CDS
TGTTCGTGGTGTATGCAAGA+TGG 0.584185 2.4:+54615695 None:intergenic
AGAAATTGAGAACTGAATCA+GGG 0.588690 2.4:+54616586 None:intergenic
CATCAACAACCAAAGCCTTG+TGG 0.591454 2.4:-54616089 MS.gene073373:CDS
CAATCTAGAAATATTTGAGT+CGG 0.591851 2.4:+54616230 None:intergenic
ATGAAGAGAAAAGGGTAATG+GGG 0.599529 2.4:+54616737 None:intergenic
ATCAACTTTCTCTTTCCACA+AGG 0.604525 2.4:+54616074 None:intergenic
TGGATGAATTAAGGGATTTG+GGG 0.606794 2.4:-54616158 MS.gene073373:CDS
TAATGTTATGTGCCACAAGA+TGG 0.610046 2.4:+54616277 None:intergenic
GAGGTAGGAAACTGCAAATG+AGG 0.610228 2.4:+54616689 None:intergenic
CACATGTGTGCAATGTCCTA+CGG 0.613554 2.4:+54616196 None:intergenic
GTTGATGCCTTTAAGAAGTG+GGG 0.614341 2.4:-54616057 MS.gene073373:CDS
GTTGGGAGTTAGAGAAACCA+TGG 0.615746 2.4:+54616556 None:intergenic
GGGTCGCAGGAAAGTAGCCA+CGG 0.625027 2.4:+54616510 None:intergenic
TGAGGTTGAATCTGAAGTGT+TGG 0.626308 2.4:+54616707 None:intergenic
CATAAAGTTAACCATGTCAG+AGG 0.632724 2.4:+54616434 None:intergenic
AGGTGCTTCTACCTCTGACA+TGG 0.645076 2.4:-54616445 MS.gene073373:CDS
GAGAAGAAATTGCACAACAG+TGG 0.646434 2.4:+54615852 None:intergenic
ATGGATGTTAACATACAATG+AGG 0.655070 2.4:+54615994 None:intergenic
ACTACGTCGTGTCATTCCCA+AGG 0.661929 2.4:-54616535 MS.gene073373:CDS
AGCTTACAGACAAGATCCTG+GGG 0.666533 2.4:+54616403 None:intergenic
GATTGCTTAGGGAACACCGT+AGG 0.718101 2.4:-54616212 MS.gene073373:CDS
GTGTCATTCCCAAGGCACCG+TGG 0.742348 2.4:-54616527 MS.gene073373:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CACTAAATTAATTTTATCAA+TGG + chr2.4:54616513-54616532 None:intergenic 15.0%
!!! AAATATTTCTAGATTGCTTA+GGG - chr2.4:54616262-54616281 MS.gene073373:CDS 20.0%
! AAACTTAACGACAAAATATC+TGG + chr2.4:54616590-54616609 None:intergenic 25.0%
! CAATCTAGAAATATTTGAGT+CGG + chr2.4:54616258-54616277 None:intergenic 25.0%
! CTATAAACCAATTCATAGAT+TGG + chr2.4:54616125-54616144 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.4:54616017-54616036 None:intergenic 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.4:54616353-54616372 MS.gene073373:CDS 25.0%
!! AGATATTTTGTCGTTAAGTT+TGG - chr2.4:54616589-54616608 MS.gene073373:CDS 25.0%
!! CAACTTTAAAAGATTAGAAG+CGG + chr2.4:54616756-54616775 None:intergenic 25.0%
!! TAATTTAGTGATGAGCTTTT+GGG - chr2.4:54616523-54616542 MS.gene073373:CDS 25.0%
!! TTAATTTAGTGATGAGCTTT+TGG - chr2.4:54616522-54616541 MS.gene073373:CDS 25.0%
!!! CAAATATTTCTAGATTGCTT+AGG - chr2.4:54616261-54616280 MS.gene073373:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.4:54616020-54616039 MS.gene073373:CDS 25.0%
AAAGCTTAGAAGCAAATTGT+GGG + chr2.4:54615842-54615861 None:intergenic 30.0%
AAATGAAGAGAAAAGGGTAA+TGG + chr2.4:54615753-54615772 None:intergenic 30.0%
AAGAAATTGAGAACTGAATC+AGG + chr2.4:54615903-54615922 None:intergenic 30.0%
AATGAAGAGAAAAGGGTAAT+GGG + chr2.4:54615752-54615771 None:intergenic 30.0%
AGAAATTGAGAACTGAATCA+GGG + chr2.4:54615902-54615921 None:intergenic 30.0%
AGATGGTCACTAAATAAAAC+AGG + chr2.4:54616194-54616213 None:intergenic 30.0%
ATGGATGTTAACATACAATG+AGG + chr2.4:54616494-54616513 None:intergenic 30.0%
GTATCAAGAAATAACTTCTC+AGG + chr2.4:54616710-54616729 None:intergenic 30.0%
GTGCAATTTCTTCTCAATAA+TGG - chr2.4:54616641-54616660 MS.gene073373:CDS 30.0%
TATCGTTCCAATCTATGAAT+TGG - chr2.4:54616115-54616134 MS.gene073373:CDS 30.0%
TGAGATTGAAAATGGATCTA+TGG + chr2.4:54615698-54615717 None:intergenic 30.0%
TGTCGTTAAGTTTGGAAAAA+AGG - chr2.4:54616597-54616616 MS.gene073373:CDS 30.0%
TTTATTTAGTGACCATCTTG+TGG - chr2.4:54616196-54616215 MS.gene073373:CDS 30.0%
! AAAGGGTTTGAGATTGAAAA+TGG + chr2.4:54615706-54615725 None:intergenic 30.0%
! ATTACATTGTTGCTTCAGAA+TGG - chr2.4:54616227-54616246 MS.gene073373:CDS 30.0%
! CTCAATTTCTTTAGAAACCA+TGG - chr2.4:54615912-54615931 MS.gene073373:CDS 30.0%
!!! ACACCAAAAGTTGTTTTTGA+AGG + chr2.4:54616359-54616378 None:intergenic 30.0%
AACTTCTCAGGCATTAAGAA+TGG + chr2.4:54616698-54616717 None:intergenic 35.0%
ATCAACTTTCTCTTTCCACA+AGG + chr2.4:54616414-54616433 None:intergenic 35.0%
ATGAAGAGAAAAGGGTAATG+GGG + chr2.4:54615751-54615770 None:intergenic 35.0%
CAAACCCAAAATTGTTGATG+AGG + chr2.4:54615818-54615837 None:intergenic 35.0%
CAAAGCTTAGAAGCAAATTG+TGG + chr2.4:54615843-54615862 None:intergenic 35.0%
CATAAAGTTAACCATGTCAG+AGG + chr2.4:54616054-54616073 None:intergenic 35.0%
GTGCAGAAATGAAGAGAAAA+GGG + chr2.4:54615759-54615778 None:intergenic 35.0%
GTGGATGAATTAAGGGATTT+GGG - chr2.4:54616326-54616345 MS.gene073373:CDS 35.0%
TAATGTTATGTGCCACAAGA+TGG + chr2.4:54616211-54616230 None:intergenic 35.0%
TGGATGAATTAAGGGATTTG+GGG - chr2.4:54616327-54616346 MS.gene073373:CDS 35.0%
! AGTTGATGCCTTTAAGAAGT+GGG - chr2.4:54616427-54616446 MS.gene073373:CDS 35.0%
! CCTACCTCATCAACAATTTT+GGG - chr2.4:54615811-54615830 MS.gene073373:CDS 35.0%
! CGACAAAATATCTGGTGTTT+TGG + chr2.4:54616582-54616601 None:intergenic 35.0%
! TCCTACCTCATCAACAATTT+TGG - chr2.4:54615810-54615829 MS.gene073373:CDS 35.0%
! TTTCGTGCAATTTTTGTTCG+TGG + chr2.4:54616807-54616826 None:intergenic 35.0%
!! AAAAGGGTAATGGGGTTATT+GGG + chr2.4:54615743-54615762 None:intergenic 35.0%
!! AAGTTGATGCCTTTAAGAAG+TGG - chr2.4:54616426-54616445 MS.gene073373:CDS 35.0%
AAGCTTACAGACAAGATCCT+GGG + chr2.4:54616086-54616105 None:intergenic 40.0%
ACTTTATGAGTAAGAACCCC+AGG - chr2.4:54616066-54616085 MS.gene073373:CDS 40.0%
GAGAAGAAATTGCACAACAG+TGG + chr2.4:54616636-54616655 None:intergenic 40.0%
GGTGCAGAAATGAAGAGAAA+AGG + chr2.4:54615760-54615779 None:intergenic 40.0%
GGTGGATGAATTAAGGGATT+TGG - chr2.4:54616325-54616344 MS.gene073373:CDS 40.0%
TAGATTCTTGCAATCGCACA+AGG - chr2.4:54616142-54616161 MS.gene073373:CDS 40.0%
TTTCTCTTTCCACAAGGCTT+TGG + chr2.4:54616408-54616427 None:intergenic 40.0%
! CCCAAAATTGTTGATGAGGT+AGG + chr2.4:54615814-54615833 None:intergenic 40.0%
! GCACACATGTGACATTTTGA+AGG - chr2.4:54616301-54616320 MS.gene073373:CDS 40.0%
! GTTGATGCCTTTAAGAAGTG+GGG - chr2.4:54616428-54616447 MS.gene073373:CDS 40.0%
! TGAGAACTGAATCAGGGTTT+TGG + chr2.4:54615896-54615915 None:intergenic 40.0%
! TGAGGTTGAATCTGAAGTGT+TGG + chr2.4:54615781-54615800 None:intergenic 40.0%
! TTTGGCAACACCCTTTTAGA+GGG + chr2.4:54615999-54616018 None:intergenic 40.0%
!! AGCTTTTGGGTCAATCAGTT+GGG - chr2.4:54616536-54616555 MS.gene073373:CDS 40.0%
!! ATGTGACATTTTGAAGGTGG+TGG - chr2.4:54616307-54616326 MS.gene073373:CDS 40.0%
!! CACATGTGACATTTTGAAGG+TGG - chr2.4:54616304-54616323 MS.gene073373:CDS 40.0%
!! GAAAAGGGTAATGGGGTTAT+TGG + chr2.4:54615744-54615763 None:intergenic 40.0%
!! GAAGGTGGTGGATGAATTAA+GGG - chr2.4:54616319-54616338 MS.gene073373:CDS 40.0%
!! GGGTTTGGTGAGAAAGTAAA+GGG + chr2.4:54615723-54615742 None:intergenic 40.0%
!! GGTTTTGGTCAGTCTTGAAA+CGG + chr2.4:54615881-54615900 None:intergenic 40.0%
!! TGAAGGTGGTGGATGAATTA+AGG - chr2.4:54616318-54616337 MS.gene073373:CDS 40.0%
!! TGGGTTTGGTGAGAAAGTAA+AGG + chr2.4:54615724-54615743 None:intergenic 40.0%
AATTGCACAACAGTGGCTCT+TGG + chr2.4:54616629-54616648 None:intergenic 45.0%
AGCTTACAGACAAGATCCTG+GGG + chr2.4:54616085-54616104 None:intergenic 45.0%
CAAGCTTACAGACAAGATCC+TGG + chr2.4:54616087-54616106 None:intergenic 45.0%
CACATGTGTGCAATGTCCTA+CGG + chr2.4:54616292-54616311 None:intergenic 45.0%
CATCAACAACCAAAGCCTTG+TGG - chr2.4:54616396-54616415 MS.gene073373:CDS 45.0%
CTTGAAACGGAGCTTGTAAG+TGG + chr2.4:54615868-54615887 None:intergenic 45.0%
GAGGTAGGAAACTGCAAATG+AGG + chr2.4:54615799-54615818 None:intergenic 45.0%
GTTGGGAGTTAGAGAAACCA+TGG + chr2.4:54615932-54615951 None:intergenic 45.0%
TGTTCGTGGTGTATGCAAGA+TGG + chr2.4:54616793-54616812 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.4:54616541-54616560 MS.gene073373:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.4:54616540-54616559 MS.gene073373:CDS 45.0%
! ATGCCTTTAAGAAGTGGGGT+TGG - chr2.4:54616432-54616451 MS.gene073373:CDS 45.0%
! CTTTAAGAAGTGGGGTTGGT+CGG - chr2.4:54616436-54616455 MS.gene073373:CDS 45.0%
! CTTTGGCAACACCCTTTTAG+AGG + chr2.4:54616000-54616019 None:intergenic 45.0%
!! GAGCTTTTGGGTCAATCAGT+TGG - chr2.4:54616535-54616554 MS.gene073373:CDS 45.0%
!! GGTAATGGGGTTATTGGGTT+TGG + chr2.4:54615738-54615757 None:intergenic 45.0%
!! TTGGCAACACCCTTTTAGAG+GGG + chr2.4:54615998-54616017 None:intergenic 45.0%
AGGTGCTTCTACCTCTGACA+TGG - chr2.4:54616040-54616059 MS.gene073373:CDS 50.0%
CGACCAACCCCACTTCTTAA+AGG + chr2.4:54616438-54616457 None:intergenic 50.0%
GATTGCTTAGGGAACACCGT+AGG - chr2.4:54616273-54616292 MS.gene073373:CDS 50.0%
GGGAATGACACGACGTAGTT+GGG + chr2.4:54615949-54615968 None:intergenic 50.0%
TGGGAATGACACGACGTAGT+TGG + chr2.4:54615950-54615969 None:intergenic 50.0%
TTTCCTGCGACCCCTCTAAA+AGG - chr2.4:54615985-54616004 MS.gene073373:CDS 50.0%
! ACTACGTCGTGTCATTCCCA+AGG - chr2.4:54615950-54615969 MS.gene073373:CDS 50.0%
! TTCCTGCGACCCCTCTAAAA+GGG - chr2.4:54615986-54616005 MS.gene073373:CDS 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.4:54616039-54616058 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+AGG - chr2.4:54616550-54616569 MS.gene073373:CDS 50.0%
GAAAGTAGCCACGGTGCCTT+GGG + chr2.4:54615969-54615988 None:intergenic 55.0%
GGAAAGTAGCCACGGTGCCT+TGG + chr2.4:54615970-54615989 None:intergenic 60.0%
GGGTCGCAGGAAAGTAGCCA+CGG + chr2.4:54615978-54615997 None:intergenic 60.0%
!! CACCCTTTTAGAGGGGTCGC+AGG + chr2.4:54615991-54616010 None:intergenic 60.0%
!! GTGTCATTCCCAAGGCACCG+TGG - chr2.4:54615958-54615977 MS.gene073373:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 54615678 54616829 54615678 ID=MS.gene073373
chr2.4 mRNA 54615678 54616829 54615678 ID=MS.gene073373.t1;Parent=MS.gene073373
chr2.4 exon 54615678 54616829 54615678 ID=MS.gene073373.t1.exon1;Parent=MS.gene073373.t1
chr2.4 CDS 54615678 54616829 54615678 ID=cds.MS.gene073373.t1;Parent=MS.gene073373.t1
Gene Sequence

>MS.gene073373

ATGTTGATTATTCATCGCCATAGATCCATTTTCAATCTCAAACCCTTTACTTTCTCACCAAACCCAATAACCCCATTACCCTTTTCTCTTCATTTCTGCACCAACACTTCAGATTCAACCTCATTTGCAGTTTCCTACCTCATCAACAATTTTGGGTTTGACCCACAATTTGCTTCTAAGCTTTGCTCCACTTACAAGCTCCGTTTCAAGACTGACCAAAACCCTGATTCAGTTCTCAATTTCTTTAGAAACCATGGTTTCTCTAACTCCCAACTACGTCGTGTCATTCCCAAGGCACCGTGGCTACTTTCCTGCGACCCCTCTAAAAGGGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGTGCTTCTACCTCTGACATGGTTAACTTTATGAGTAAGAACCCCAGGATCTTGTCTGTAAGCTTGAAGAATCGTATCGTTCCAATCTATGAATTGGTTTATAGATTCTTGCAATCGCACAAGGACGCTATTGCTTGTGTAATTAGCAATCCTGTTTTATTTAGTGACCATCTTGTGGCACATAACATTACATTGTTGCTTCAGAATGGACTCTCCGACTCAAATATTTCTAGATTGCTTAGGGAACACCGTAGGACATTGCACACATGTGACATTTTGAAGGTGGTGGATGAATTAAGGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCTTTGATAGCGAAATCATCAACAACCAAAGCCTTGTGGAAAGAGAAAGTTGATGCCTTTAAGAAGTGGGGTTGGTCGGATGAAGATGTTCTTGAAGCATTTAAAAAGCAACCTCATTGTATGTTAACATCCATTGATAAAATTAATTTAGTGATGAGCTTTTGGGTCAATCAGTTGGGTTGGGATGCTAAGGACATTGCCAAAACACCAGATATTTTGTCGTTAAGTTTGGAAAAAAGGATCATTCCAAGAGCCACTGTTGTGCAATTTCTTCTCAATAATGGTTTGCGAAATAAGAATTCAAGCTTAATTTATCCATTCTTAATGCCTGAGAAGTTATTTCTTGATACGTTTGTAAAACGTTTTGAGAATGCCGCTTCTAATCTTTTAAAGTTGTATGAAGAAAAACTCCATCTTGCATACACCACGAACAAAAATTGCACGAAATGA
Protein sequence

>MS.gene073373.t1

MLIIHRHRSIFNLKPFTFSPNPITPLPFSLHFCTNTSDSTSFAVSYLINNFGFDPQFASKLCSTYKLRFKTDQNPDSVLNFFRNHGFSNSQLRRVIPKAPWLLSCDPSKRVLPKFQFFLSKGASTSDMVNFMSKNPRILSVSLKNRIVPIYELVYRFLQSHKDAIACVISNPVLFSDHLVAHNITLLLQNGLSDSNISRLLREHRRTLHTCDILKVVDELRDLGFNPSKTTFGVALIAKSSTTKALWKEKVDAFKKWGWSDEDVLEAFKKQPHCMLTSIDKINLVMSFWVNQLGWDAKDIAKTPDILSLSLEKRIIPRATVVQFLLNNGLRNKNSSLIYPFLMPEKLFLDTFVKRFENAASNLLKLYEEKLHLAYTTNKNCTK