AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004540.t1 MTR_2g436400 95.276 381 18 0 1 381 1 381 0.0 746
MS.gene004540.t1 MTR_2g437060 74.936 391 86 3 1 381 1 389 0.0 581
MS.gene004540.t1 MTR_2g437080 70.400 375 103 3 10 381 5 374 0.0 509
MS.gene004540.t1 MTR_2g437020 66.755 379 116 4 5 381 4 374 5.17e-176 495
MS.gene004540.t1 MTR_2g437030 67.192 381 113 2 10 381 83 460 1.03e-173 492
MS.gene004540.t1 MTR_2g436440 64.433 388 123 3 1 381 1 380 9.51e-173 487
MS.gene004540.t1 MTR_2g437040 63.483 356 129 1 27 381 19 374 1.68e-159 453
MS.gene004540.t1 MTR_4g119550 51.765 340 161 2 43 381 47 384 1.73e-122 359
MS.gene004540.t1 MTR_2g437170 50.130 385 173 5 16 381 10 394 3.34e-121 357
MS.gene004540.t1 MTR_2g437150 50.000 380 171 5 16 376 10 389 4.46e-119 351
MS.gene004540.t1 MTR_2g437960 49.326 371 172 3 27 381 20 390 9.97e-119 350
MS.gene004540.t1 MTR_2g437960 49.326 371 172 3 27 381 20 390 1.72e-118 350
MS.gene004540.t1 MTR_2g437120 49.864 369 168 4 25 377 16 383 1.13e-116 345
MS.gene004540.t1 MTR_2g438010 50.862 348 160 3 43 381 62 407 5.58e-116 344
MS.gene004540.t1 MTR_2g437160 48.794 373 175 4 25 381 27 399 8.99e-115 340
MS.gene004540.t1 MTR_2g437100 51.087 368 157 6 33 377 28 395 8.52e-113 335
MS.gene004540.t1 MTR_2g437200 47.200 375 158 3 41 377 26 398 4.06e-112 333
MS.gene004540.t1 MTR_2g437180 50.585 342 162 4 39 377 43 380 4.99e-112 333
MS.gene004540.t1 MTR_2g076320 49.573 351 160 3 43 381 42 387 2.04e-111 332
MS.gene004540.t1 MTR_2g437130 47.849 372 160 7 29 381 19 375 5.13e-111 330
MS.gene004540.t1 MTR_2g437990 48.986 345 170 2 43 381 42 386 3.65e-110 329
MS.gene004540.t1 MTR_2g437240 45.503 378 166 3 42 381 27 402 1.07e-107 322
MS.gene004540.t1 MTR_2g437260 46.726 336 175 2 43 376 32 365 9.69e-107 319
MS.gene004540.t1 MTR_2g436460 46.491 342 180 2 37 377 37 376 2.28e-106 318
MS.gene004540.t1 MTR_2g019840 47.354 378 186 8 10 381 5 375 9.92e-104 311
MS.gene004540.t1 MTR_2g438020 46.216 370 159 3 46 377 70 437 1.71e-102 312
MS.gene004540.t1 MTR_2g436370 77.041 196 44 1 1 196 1 195 2.21e-102 301
MS.gene004540.t1 MTR_2g437940 46.216 370 159 5 25 381 16 358 2.04e-101 305
MS.gene004540.t1 MTR_2g019810 46.407 334 173 4 48 379 32 361 9.41e-96 290
MS.gene004540.t1 MTR_4g120380 44.575 341 185 2 37 375 32 370 1.52e-95 290
MS.gene004540.t1 MTR_4g119580 43.149 343 193 1 33 375 30 370 3.18e-93 284
MS.gene004540.t1 MTR_4g120380 44.860 321 173 2 37 355 32 350 2.53e-89 276
MS.gene004540.t1 MTR_2g437090 69.136 162 50 0 220 381 1 162 7.82e-77 235
MS.gene004540.t1 MTR_2g437160 53.571 196 88 1 189 381 113 308 4.12e-65 210
MS.gene004540.t1 MTR_2g437160 40.157 127 58 4 25 137 27 149 1.05e-16 80.5
MS.gene004540.t1 MTR_3g450500 32.738 336 214 5 48 375 39 370 8.13e-54 182
MS.gene004540.t1 MTR_4g119570 38.462 247 119 2 127 373 14 227 7.33e-50 167
MS.gene004540.t1 MTR_4g007490 31.250 352 232 6 35 379 24 372 3.15e-49 170
MS.gene004540.t1 MTR_8g080990 29.492 295 205 2 80 371 74 368 4.69e-37 138
MS.gene004540.t1 MTR_8g081000 26.899 316 228 2 63 375 70 385 1.08e-33 129
MS.gene004540.t1 MTR_8g081000 26.899 316 228 2 63 375 70 385 1.10e-33 129
MS.gene004540.t1 MTR_8g081000 26.899 316 228 2 63 375 70 385 1.81e-33 129
MS.gene004540.t1 MTR_2g436380 55.752 113 43 3 220 332 1 106 1.65e-30 118
MS.gene004540.t1 MTR_4g049400 42.105 114 62 1 39 148 38 151 1.40e-20 90.1
MS.gene004540.t1 MTR_3g085240 62.264 53 20 0 279 331 176 228 2.12e-15 75.9
MS.gene004540.t1 MTR_3g085240 31.757 148 89 3 22 169 35 170 1.68e-11 64.3
MS.gene004540.t1 MTR_4g119615 60.000 50 20 0 319 368 26 75 7.70e-14 67.4
MS.gene004540.t1 MTR_2g019830 55.385 65 28 1 313 377 136 199 1.77e-11 63.5
MS.gene004540.t1 MTR_8g012210 20.137 293 229 4 92 380 62 353 3.02e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004540.t1 AT5G07900 34.234 333 211 4 48 374 69 399 1.41e-64 211
MS.gene004540.t1 AT1G21150 29.714 350 234 6 37 378 9 354 2.78e-49 170
MS.gene004540.t1 AT1G21150 30.088 339 225 6 48 378 56 390 1.38e-47 166
MS.gene004540.t1 AT1G21150 30.088 339 225 6 48 378 120 454 6.07e-47 166
MS.gene004540.t1 AT1G61980 27.855 359 214 7 48 366 52 405 5.74e-38 141
MS.gene004540.t1 AT1G61980 27.855 359 214 7 48 366 52 405 5.74e-38 141
MS.gene004540.t1 AT1G61980 27.855 359 214 7 48 366 52 405 5.74e-38 141
MS.gene004540.t1 AT1G62120 25.990 404 245 10 8 366 17 411 1.52e-34 132
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61970 26.382 398 241 8 10 360 7 399 2.75e-34 131
MS.gene004540.t1 AT1G61990 27.596 366 211 8 51 374 55 408 4.03e-32 125
MS.gene004540.t1 AT1G61990 27.596 366 211 8 51 374 55 408 4.03e-32 125
MS.gene004540.t1 AT5G64950 29.526 359 228 9 36 375 24 376 7.93e-32 124
MS.gene004540.t1 AT1G62010 25.776 322 198 6 83 366 71 389 9.24e-29 116
MS.gene004540.t1 AT3G46950 28.912 294 181 7 8 292 17 291 2.81e-27 112
MS.gene004540.t1 AT1G62110 26.712 292 192 5 8 290 17 295 8.92e-27 111
MS.gene004540.t1 AT1G62085 27.525 396 205 14 48 366 55 445 4.17e-26 109
MS.gene004540.t1 AT1G62085 27.525 396 205 14 48 366 55 445 4.17e-26 109
MS.gene004540.t1 AT1G61960 30.290 241 164 3 51 289 55 293 5.07e-25 106
MS.gene004540.t1 AT5G23930 21.882 425 259 9 8 365 18 436 4.00e-24 103
MS.gene004540.t1 AT1G62150 27.344 256 171 6 42 289 52 300 8.60e-21 94.0
MS.gene004540.t1 AT1G56380 22.616 367 226 12 49 366 28 385 6.22e-14 73.2
MS.gene004540.t1 AT1G56380 22.616 367 226 12 49 366 17 374 6.37e-14 73.2

Find 79 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAATCTGACAAGGAAATTT+TGG 0.116874 2.1:-56963841 MS.gene004540:CDS
GGAAAAGAGGGTTAGGGTTT+GGG 0.197496 2.1:+56964249 None:intergenic
TAAATTTAGTGATGAACTTT+TGG 0.199642 2.1:-56963470 MS.gene004540:CDS
GTAAAGGAATTAAAGGATTT+GGG 0.236314 2.1:-56963666 MS.gene004540:CDS
CTCACATTCTTTAGAAACTA+TGG 0.241468 2.1:-56964080 MS.gene004540:CDS
AGACCAACCCCATTTCTTAA+AGG 0.251614 2.1:+56963557 None:intergenic
GGTATTGTTTACTGTTGTTT+TGG 0.253046 2.1:+56963602 None:intergenic
GGTAAAGGAATTAAAGGATT+TGG 0.272443 2.1:-56963667 MS.gene004540:CDS
AAACTTAATGACAAAATATA+AGG 0.279924 2.1:+56963405 None:intergenic
ATGGATGTTAACATAGAATA+AGG 0.283748 2.1:+56963501 None:intergenic
ATGGTAGGTGGAACAGAGTT+TGG 0.292000 2.1:+56964139 None:intergenic
TCCTACCTCATCAACAATAT+TGG 0.301774 2.1:-56964182 MS.gene004540:CDS
AAACTTTGGCAACACAGTTT+TGG 0.307791 2.1:+56963992 None:intergenic
TGGAAAAGAGGGTTAGGGTT+TGG 0.315934 2.1:+56964248 None:intergenic
TGACAAAATATAAGGTTGTT+TGG 0.318444 2.1:+56963413 None:intergenic
TCCCTTGAGATAATTGATTA+GGG 0.328607 2.1:+56964298 None:intergenic
ATCCCTTGAGATAATTGATT+AGG 0.337905 2.1:+56964297 None:intergenic
CAAGTCGGTAAAGGAATTAA+AGG 0.349745 2.1:-56963673 MS.gene004540:CDS
TGTTCAACATCAATTCTTCA+TGG 0.364493 2.1:-56964334 MS.gene004540:CDS
TTGGGTCAATCAGTTGGGTT+GGG 0.365374 2.1:-56963451 MS.gene004540:CDS
AAGTTGATGCCTTTAAGAAA+TGG 0.366199 2.1:-56963566 MS.gene004540:CDS
GAAAAGAGGGTTAGGGTTTG+GGG 0.370673 2.1:+56964250 None:intergenic
AGTTGATGCCTTTAAGAAAT+GGG 0.371481 2.1:-56963565 MS.gene004540:CDS
TTTGGGGATTGTGATGTTAG+AGG 0.390306 2.1:+56964266 None:intergenic
AAATGGTGGAAAAGAGGGTT+AGG 0.393469 2.1:+56964242 None:intergenic
GTCAGAGGTAGAAGCGCCTT+TGG 0.405353 2.1:+56963956 None:intergenic
AACTTATCAGTCACTACAAA+TGG 0.416436 2.1:+56963297 None:intergenic
TTGGGGATTGTGATGTTAGA+GGG 0.419030 2.1:+56964267 None:intergenic
CATCAACTTTCTCTTTCCAT+AGG 0.426803 2.1:+56963580 None:intergenic
TGGCAATCATATCGCGTAGT+TGG 0.438979 2.1:+56964045 None:intergenic
GGTTTGCAGGAAAGCAGCTC+TGG 0.441213 2.1:+56964017 None:intergenic
AAGAATGTGAGAACAGAATC+AGG 0.451252 2.1:+56964092 None:intergenic
GGCAATCATATCGCGTAGTT+GGG 0.453430 2.1:+56964046 None:intergenic
CTGAAGTGTTGGTGCAGAAA+TGG 0.455082 2.1:+56964225 None:intergenic
ATCACAATGCTGATTGAGAA+TGG 0.457142 2.1:-56963765 MS.gene004540:CDS
AATAGTCCCAGCCTATGAAT+TGG 0.461317 2.1:-56963877 MS.gene004540:CDS
GGAAAGCAGCTCTGGCATCT+TGG 0.471973 2.1:+56964025 None:intergenic
GAATCTGTACACCAATTCAT+AGG 0.473956 2.1:+56963866 None:intergenic
TTTGGGTCAATCAGTTGGGT+TGG 0.480856 2.1:-56963452 MS.gene004540:CDS
CTGTACACCAATTCATAGGC+TGG 0.485721 2.1:+56963870 None:intergenic
CAGAGTTTGGAAGCAGATTG+TGG 0.492532 2.1:+56964152 None:intergenic
CTGGGACTATTTGATTGTTG+AGG 0.502053 2.1:+56963889 None:intergenic
AACCCTAATCAATTATCTCA+AGG 0.502785 2.1:-56964300 MS.gene004540:CDS
TGGGGATTGTGATGTTAGAG+GGG 0.504801 2.1:+56964268 None:intergenic
TCAGTTGGGTTGGGATGCTA+TGG 0.506950 2.1:-56963442 MS.gene004540:CDS
TCACAATGCTGATTGAGAAT+GGG 0.508211 2.1:-56963764 MS.gene004540:CDS
AATATAAGGTTGTTTGGCAA+TGG 0.508469 2.1:+56963419 None:intergenic
AGAGTTTGGAAGCAGATTGT+GGG 0.513374 2.1:+56964153 None:intergenic
TGCAGAAATGGTGGAAAAGA+GGG 0.515244 2.1:+56964237 None:intergenic
GGGTGGTCTCGAAACGAAGA+TGG 0.518421 2.1:+56964120 None:intergenic
ATCAACTTTCTCTTTCCATA+GGG 0.521600 2.1:+56963581 None:intergenic
CAACAGTAAACAATACCCTA+TGG 0.530932 2.1:-56963596 MS.gene004540:CDS
TGACATGCTCAAGTCGGTAA+AGG 0.531848 2.1:-56963682 MS.gene004540:CDS
GTGCAGAAATGGTGGAAAAG+AGG 0.534389 2.1:+56964236 None:intergenic
GTGCAATTTCTTCTCAACAA+AGG 0.536369 2.1:-56963351 MS.gene004540:CDS
AATGGTGGAAAAGAGGGTTA+GGG 0.547641 2.1:+56964243 None:intergenic
AATTGCACAACAGCGGCCCT+CGG 0.562081 2.1:+56963366 None:intergenic
CAAAACCAATATTGTTGATG+AGG 0.562530 2.1:+56964177 None:intergenic
AGGCTTACATACAAGACTCT+AGG 0.563542 2.1:+56963909 None:intergenic
TAAAGGAATTAAAGGATTTG+GGG 0.564846 2.1:-56963665 MS.gene004540:CDS
CATTGCTGAGGGAACAGACT+CGG 0.569500 2.1:-56963719 MS.gene004540:CDS
ACCCTAATCAATTATCTCAA+GGG 0.570256 2.1:-56964299 MS.gene004540:CDS
GAGGTAGGAGACTGCAAGAG+AGG 0.570893 2.1:+56964196 None:intergenic
GTTGATGCCTTTAAGAAATG+GGG 0.573670 2.1:-56963564 MS.gene004540:CDS
CAGATTCTTGCAATCTGACA+AGG 0.575407 2.1:-56963850 MS.gene004540:CDS
ACCAATATTGTTGATGAGGT+AGG 0.575620 2.1:+56964181 None:intergenic
AAACATTAAATCATTGCTGA+GGG 0.598387 2.1:-56963730 MS.gene004540:CDS
ATGCCTTTAAGAAATGGGGT+TGG 0.598819 2.1:-56963560 MS.gene004540:CDS
TGTACACCAATTCATAGGCT+GGG 0.603503 2.1:+56963871 None:intergenic
AATTGATTAGGGTTCTTCTG+TGG 0.610118 2.1:+56964309 None:intergenic
CAAACATTAAATCATTGCTG+AGG 0.611140 2.1:-56963731 MS.gene004540:CDS
GACAATGTTAACAATGTCAG+AGG 0.615619 2.1:+56963941 None:intergenic
AGAGGTTGAATCTGAAGTGT+TGG 0.626308 2.1:+56964214 None:intergenic
GGTCTCGAAACGAAGATGGT+AGG 0.628439 2.1:+56964124 None:intergenic
CACAATGCTGATTGAGAATG+GGG 0.632348 2.1:-56963763 MS.gene004540:CDS
CTCGAAACGAAGATGGTAGG+TGG 0.633297 2.1:+56964127 None:intergenic
GAGAAGAAATTGCACAACAG+CGG 0.635644 2.1:+56963359 None:intergenic
AAGTGTTGGTGCAGAAATGG+TGG 0.640434 2.1:+56964228 None:intergenic
GAGACGTGACATGCTCAAGT+CGG 0.669830 2.1:-56963688 MS.gene004540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTTAATGACAAAATATA+AGG + chr2.1:56964145-56964164 None:intergenic 15.0%
!!! CACTAAATTTATTTTATCAA+TGG + chr2.1:56964068-56964087 None:intergenic 15.0%
!!! TTATATTTTGTCATTAAGTT+TGG - chr2.1:56964144-56964163 MS.gene004540:CDS 15.0%
!!! AAATTTAGTGATGAACTTTT+GGG - chr2.1:56964078-56964097 MS.gene004540:CDS 20.0%
!!! TAAATTTAGTGATGAACTTT+TGG - chr2.1:56964077-56964096 MS.gene004540:CDS 20.0%
!!! TTATCTTTTAAAGTTGTATG+AGG - chr2.1:56964315-56964334 MS.gene004540:CDS 20.0%
! AAACATTAAATCATTGCTGA+GGG - chr2.1:56963817-56963836 MS.gene004540:CDS 25.0%
! ATGGATGTTAACATAGAATA+AGG + chr2.1:56964049-56964068 None:intergenic 25.0%
! GAGAGAAAAAATTGAAACTT+TGG + chr2.1:56963572-56963591 None:intergenic 25.0%
! GTAAAGGAATTAAAGGATTT+GGG - chr2.1:56963881-56963900 MS.gene004540:CDS 25.0%
! TAAAGGAATTAAAGGATTTG+GGG - chr2.1:56963882-56963901 MS.gene004540:CDS 25.0%
! TGACAAAATATAAGGTTGTT+TGG + chr2.1:56964137-56964156 None:intergenic 25.0%
! TGTCATTAAGTTTGGAAAAA+AGG - chr2.1:56964152-56964171 MS.gene004540:CDS 25.0%
!! AATCCTTCAAAAACAACTTT+TGG - chr2.1:56963908-56963927 MS.gene004540:CDS 25.0%
!!! TATAAAACGTTTTGAGAATG+AGG - chr2.1:56964288-56964307 MS.gene004540:CDS 25.0%
!!! TTTCAATTTTTTCTCTCCAA+AGG - chr2.1:56963575-56963594 MS.gene004540:CDS 25.0%
AACCCTAATCAATTATCTCA+AGG - chr2.1:56963247-56963266 MS.gene004540:CDS 30.0%
AACTTATCAGTCACTACAAA+TGG + chr2.1:56964253-56964272 None:intergenic 30.0%
AATATAAGGTTGTTTGGCAA+TGG + chr2.1:56964131-56964150 None:intergenic 30.0%
ACCCTAATCAATTATCTCAA+GGG - chr2.1:56963248-56963267 MS.gene004540:CDS 30.0%
ATCAACTTTCTCTTTCCATA+GGG + chr2.1:56963969-56963988 None:intergenic 30.0%
ATCCCTTGAGATAATTGATT+AGG + chr2.1:56963253-56963272 None:intergenic 30.0%
CAAAACCAATATTGTTGATG+AGG + chr2.1:56963373-56963392 None:intergenic 30.0%
CAAACATTAAATCATTGCTG+AGG - chr2.1:56963816-56963835 MS.gene004540:CDS 30.0%
GGTAAAGGAATTAAAGGATT+TGG - chr2.1:56963880-56963899 MS.gene004540:CDS 30.0%
TCCCTTGAGATAATTGATTA+GGG + chr2.1:56963252-56963271 None:intergenic 30.0%
TGTTCAACATCAATTCTTCA+TGG - chr2.1:56963213-56963232 MS.gene004540:CDS 30.0%
! AGTTGATGCCTTTAAGAAAT+GGG - chr2.1:56963982-56964001 MS.gene004540:CDS 30.0%
! CTCACATTCTTTAGAAACTA+TGG - chr2.1:56963467-56963486 MS.gene004540:CDS 30.0%
!! AAGTTGATGCCTTTAAGAAA+TGG - chr2.1:56963981-56964000 MS.gene004540:CDS 30.0%
!! GGTATTGTTTACTGTTGTTT+TGG + chr2.1:56963948-56963967 None:intergenic 30.0%
!!! ACACCAAAAGTTGTTTTTGA+AGG + chr2.1:56963914-56963933 None:intergenic 30.0%
AAGAATGTGAGAACAGAATC+AGG + chr2.1:56963458-56963477 None:intergenic 35.0%
AATTGATTAGGGTTCTTCTG+TGG + chr2.1:56963241-56963260 None:intergenic 35.0%
CAACAGTAAACAATACCCTA+TGG - chr2.1:56963951-56963970 MS.gene004540:CDS 35.0%
CAAGTCGGTAAAGGAATTAA+AGG - chr2.1:56963874-56963893 MS.gene004540:CDS 35.0%
CATCAACTTTCTCTTTCCAT+AGG + chr2.1:56963970-56963989 None:intergenic 35.0%
GAATCTGTACACCAATTCAT+AGG + chr2.1:56963684-56963703 None:intergenic 35.0%
GACAATGTTAACAATGTCAG+AGG + chr2.1:56963609-56963628 None:intergenic 35.0%
GTGCAATTTCTTCTCAACAA+AGG - chr2.1:56964196-56964215 MS.gene004540:CDS 35.0%
TATGAGGAAAAACGCAATCT+AGG - chr2.1:56964331-56964350 MS.gene004540:CDS 35.0%
TCCTACCTCATCAACAATAT+TGG - chr2.1:56963365-56963384 MS.gene004540:CDS 35.0%
! AAACTTTGGCAACACAGTTT+TGG + chr2.1:56963558-56963577 None:intergenic 35.0%
! ACCAATATTGTTGATGAGGT+AGG + chr2.1:56963369-56963388 None:intergenic 35.0%
! ATCACAATGCTGATTGAGAA+TGG - chr2.1:56963782-56963801 MS.gene004540:CDS 35.0%
! GAGAACAGAATCAGGTTTTT+GGG + chr2.1:56963450-56963469 None:intergenic 35.0%
! GCAATCTGACAAGGAAATTT+TGG - chr2.1:56963706-56963725 MS.gene004540:CDS 35.0%
! GTTGATGCCTTTAAGAAATG+GGG - chr2.1:56963983-56964002 MS.gene004540:CDS 35.0%
! TCACAATGCTGATTGAGAAT+GGG - chr2.1:56963783-56963802 MS.gene004540:CDS 35.0%
! TGAGAACAGAATCAGGTTTT+TGG + chr2.1:56963451-56963470 None:intergenic 35.0%
!! AACTTTTGGGTCAATCAGTT+GGG - chr2.1:56964091-56964110 MS.gene004540:CDS 35.0%
AATAGTCCCAGCCTATGAAT+TGG - chr2.1:56963670-56963689 MS.gene004540:CDS 40.0%
AGACCAACCCCATTTCTTAA+AGG + chr2.1:56963993-56964012 None:intergenic 40.0%
AGAGTTTGGAAGCAGATTGT+GGG + chr2.1:56963397-56963416 None:intergenic 40.0%
AGGCTTACATACAAGACTCT+AGG + chr2.1:56963641-56963660 None:intergenic 40.0%
CAGATTCTTGCAATCTGACA+AGG - chr2.1:56963697-56963716 MS.gene004540:CDS 40.0%
CTGGGACTATTTGATTGTTG+AGG + chr2.1:56963661-56963680 None:intergenic 40.0%
GAGAAGAAATTGCACAACAG+CGG + chr2.1:56964191-56964210 None:intergenic 40.0%
GGAAAAAAGGATCATTCCGA+GGG - chr2.1:56964165-56964184 MS.gene004540:CDS 40.0%
TGGAAAAAAGGATCATTCCG+AGG - chr2.1:56964164-56964183 MS.gene004540:CDS 40.0%
TGTACACCAATTCATAGGCT+GGG + chr2.1:56963679-56963698 None:intergenic 40.0%
TTGGGGATTGTGATGTTAGA+GGG + chr2.1:56963283-56963302 None:intergenic 40.0%
TTTGGGGATTGTGATGTTAG+AGG + chr2.1:56963284-56963303 None:intergenic 40.0%
! AAATGGTGGAAAAGAGGGTT+AGG + chr2.1:56963308-56963327 None:intergenic 40.0%
! AATGGTGGAAAAGAGGGTTA+GGG + chr2.1:56963307-56963326 None:intergenic 40.0%
! AGAGGTTGAATCTGAAGTGT+TGG + chr2.1:56963336-56963355 None:intergenic 40.0%
! ATGCCTTTAAGAAATGGGGT+TGG - chr2.1:56963987-56964006 MS.gene004540:CDS 40.0%
! CACAATGCTGATTGAGAATG+GGG - chr2.1:56963784-56963803 MS.gene004540:CDS 40.0%
! TGCAGAAATGGTGGAAAAGA+GGG + chr2.1:56963313-56963332 None:intergenic 40.0%
!! AACAGAATCAGGTTTTTGGG+TGG + chr2.1:56963447-56963466 None:intergenic 40.0%
!! GAACTTTTGGGTCAATCAGT+TGG - chr2.1:56964090-56964109 MS.gene004540:CDS 40.0%
!!! TTTGGCAACACAGTTTTGGA+GGG + chr2.1:56963554-56963573 None:intergenic 40.0%
ATGGTAGGTGGAACAGAGTT+TGG + chr2.1:56963411-56963430 None:intergenic 45.0%
CAGAGTTTGGAAGCAGATTG+TGG + chr2.1:56963398-56963417 None:intergenic 45.0%
CTGTACACCAATTCATAGGC+TGG + chr2.1:56963680-56963699 None:intergenic 45.0%
GAAAAGAGGGTTAGGGTTTG+GGG + chr2.1:56963300-56963319 None:intergenic 45.0%
GGAAAAGAGGGTTAGGGTTT+GGG + chr2.1:56963301-56963320 None:intergenic 45.0%
GGCAATCATATCGCGTAGTT+GGG + chr2.1:56963504-56963523 None:intergenic 45.0%
TGACATGCTCAAGTCGGTAA+AGG - chr2.1:56963865-56963884 MS.gene004540:CDS 45.0%
TGGAAAAGAGGGTTAGGGTT+TGG + chr2.1:56963302-56963321 None:intergenic 45.0%
TGGCAATCATATCGCGTAGT+TGG + chr2.1:56963505-56963524 None:intergenic 45.0%
TGGGGATTGTGATGTTAGAG+GGG + chr2.1:56963282-56963301 None:intergenic 45.0%
TTGGGTCAATCAGTTGGGTT+GGG - chr2.1:56964096-56964115 MS.gene004540:CDS 45.0%
TTTGGGTCAATCAGTTGGGT+TGG - chr2.1:56964095-56964114 MS.gene004540:CDS 45.0%
! GTGCAGAAATGGTGGAAAAG+AGG + chr2.1:56963314-56963333 None:intergenic 45.0%
!! AAGTGTTGGTGCAGAAATGG+TGG + chr2.1:56963322-56963341 None:intergenic 45.0%
!! CTGAAGTGTTGGTGCAGAAA+TGG + chr2.1:56963325-56963344 None:intergenic 45.0%
!! CTTTGGCAACACAGTTTTGG+AGG + chr2.1:56963555-56963574 None:intergenic 45.0%
CATTGCTGAGGGAACAGACT+CGG - chr2.1:56963828-56963847 MS.gene004540:CDS 50.0%
CTCGAAACGAAGATGGTAGG+TGG + chr2.1:56963423-56963442 None:intergenic 50.0%
GAGACGTGACATGCTCAAGT+CGG - chr2.1:56963859-56963878 MS.gene004540:CDS 50.0%
GGTCTCGAAACGAAGATGGT+AGG + chr2.1:56963426-56963445 None:intergenic 50.0%
!! TCAGTTGGGTTGGGATGCTA+TGG - chr2.1:56964105-56964124 MS.gene004540:CDS 50.0%
!!! CACAGTTTTGGAGGGTTTGC+AGG + chr2.1:56963546-56963565 None:intergenic 50.0%
AATTGCACAACAGCGGCCCT+CGG + chr2.1:56964184-56964203 None:intergenic 55.0%
GAGGTAGGAGACTGCAAGAG+AGG + chr2.1:56963354-56963373 None:intergenic 55.0%
GGGTGGTCTCGAAACGAAGA+TGG + chr2.1:56963430-56963449 None:intergenic 55.0%
GGTTTGCAGGAAAGCAGCTC+TGG + chr2.1:56963533-56963552 None:intergenic 55.0%
! GGAAAGCAGCTCTGGCATCT+TGG + chr2.1:56963525-56963544 None:intergenic 55.0%
!! GTCAGAGGTAGAAGCGCCTT+TGG + chr2.1:56963594-56963613 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 56963212 56964357 56963212 ID=MS.gene004540
chr2.1 mRNA 56963212 56964357 56963212 ID=MS.gene004540.t1;Parent=MS.gene004540
chr2.1 exon 56963212 56964357 56963212 ID=MS.gene004540.t1.exon1;Parent=MS.gene004540.t1
chr2.1 CDS 56963212 56964357 56963212 ID=cds.MS.gene004540.t1;Parent=MS.gene004540.t1
Gene Sequence

>MS.gene004540

ATGTTCAACATCAATTCTTCATGGCGCCACAGAAGAACCCTAATCAATTATCTCAAGGGATTCACTACCCCTCTAACATCACAATCCCCAAACCCTAACCCTCTTTTCCACCATTTCTGCACCAACACTTCAGATTCAACCTCTCTTGCAGTCTCCTACCTCATCAACAATATTGGTTTTGACCCACAATCTGCTTCCAAACTCTGTTCCACCTACCATCTTCGTTTCGAGACCACCCAAAAACCTGATTCTGTTCTCACATTCTTTAGAAACTATGGATTCTCTAATTCCCAACTACGCGATATGATTGCCAAGATGCCAGAGCTGCTTTCCTGCAAACCCTCCAAAACTGTGTTGCCAAAGTTTCAATTTTTTCTCTCCAAAGGCGCTTCTACCTCTGACATTGTTAACATTGTCACTAAGACTCCTAGAGTCTTGTATGTAAGCCTCAACAATCAAATAGTCCCAGCCTATGAATTGGTGTACAGATTCTTGCAATCTGACAAGGAAATTTTGGAAAAGTTGAATTACAGTGCAAATTTACTTTGTGATTGTACTGTGCAAAATAACATCACAATGCTGATTGAGAATGGGGTGTCTGACTCAAACATTAAATCATTGCTGAGGGAACAGACTCGGATATTCAAGAGACGTGACATGCTCAAGTCGGTAAAGGAATTAAAGGATTTGGGGTTTAATCCTTCAAAAACAACTTTTGGTGTAGCATTGCATGCCAAAACAACAGTAAACAATACCCTATGGAAAGAGAAAGTTGATGCCTTTAAGAAATGGGGTTGGTCTGAAGAAGATTCTCTCGAAGCATTTAGAAAGAAACCTTATTCTATGTTAACATCCATTGATAAAATAAATTTAGTGATGAACTTTTGGGTCAATCAGTTGGGTTGGGATGCTATGGCCATTGCCAAACAACCTTATATTTTGTCATTAAGTTTGGAAAAAAGGATCATTCCGAGGGCCGCTGTTGTGCAATTTCTTCTCAACAAAGGTTTGACAGATAAGAACGCAAGCTTAACTTATCCATTTGTAGTGACTGATAAGTTGTTTCTTAATATGTTTATAAAACGTTTTGAGAATGAGGCTTCTTATCTTTTAAAGTTGTATGAGGAAAAACGCAATCTAGGATAG
Protein sequence

>MS.gene004540.t1

MFNINSSWRHRRTLINYLKGFTTPLTSQSPNPNPLFHHFCTNTSDSTSLAVSYLINNIGFDPQSASKLCSTYHLRFETTQKPDSVLTFFRNYGFSNSQLRDMIAKMPELLSCKPSKTVLPKFQFFLSKGASTSDIVNIVTKTPRVLYVSLNNQIVPAYELVYRFLQSDKEILEKLNYSANLLCDCTVQNNITMLIENGVSDSNIKSLLREQTRIFKRRDMLKSVKELKDLGFNPSKTTFGVALHAKTTVNNTLWKEKVDAFKKWGWSEEDSLEAFRKKPYSMLTSIDKINLVMNFWVNQLGWDAMAIAKQPYILSLSLEKRIIPRAAVVQFLLNKGLTDKNASLTYPFVVTDKLFLNMFIKRFENEASYLLKLYEEKRNLG