AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07481

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07481.t1 MTR_1g077540 97.287 258 7 0 1 258 1 258 0.0 504
MS.gene07481.t1 MTR_6g069040 79.457 258 50 1 1 258 1 255 3.19e-143 402
MS.gene07481.t1 MTR_1g115405 53.876 258 113 1 1 258 1 252 2.44e-95 280
MS.gene07481.t1 MTR_7g023350 53.876 258 113 1 1 258 1 252 1.60e-92 273
MS.gene07481.t1 MTR_4g063090 53.488 258 114 1 1 258 1 252 3.96e-92 272
MS.gene07481.t1 MTR_2g101370 52.174 253 114 1 4 256 3 248 4.42e-89 264
MS.gene07481.t1 MTR_7g103030 53.876 258 111 2 1 258 1 250 7.39e-88 261
MS.gene07481.t1 MTR_8g013680 49.600 250 119 1 7 256 5 247 2.69e-82 247
MS.gene07481.t1 MTR_5g012810 50.833 240 117 1 5 244 3 241 2.22e-72 222
MS.gene07481.t1 MTR_1g006490 44.269 253 130 3 5 257 3 244 1.78e-70 217
MS.gene07481.t1 MTR_1g006590 43.874 253 131 3 5 257 3 244 3.18e-66 206
MS.gene07481.t1 MTR_2g060360 40.343 233 138 1 10 242 8 239 2.40e-53 173
MS.gene07481.t1 MTR_2g094270 37.705 244 135 6 11 238 30 272 1.04e-37 134
MS.gene07481.t1 MTR_3g118010 38.865 229 126 5 21 238 37 262 8.31e-37 131
MS.gene07481.t1 MTR_7g101190 37.768 233 129 6 21 238 39 270 7.43e-36 129
MS.gene07481.t1 MTR_4g059390 38.197 233 128 6 21 238 39 270 3.55e-35 127
MS.gene07481.t1 MTR_8g098375 36.522 230 131 5 21 238 52 278 1.10e-34 126
MS.gene07481.t1 MTR_5g010150 36.087 230 132 5 21 238 52 278 1.28e-33 123
MS.gene07481.t1 MTR_3g070210 35.146 239 140 5 21 247 55 290 1.15e-32 121
MS.gene07481.t1 MTR_5g082070 36.087 230 132 5 21 238 55 281 1.95e-32 120
MS.gene07481.t1 MTR_4g415300 36.364 231 129 7 21 238 56 281 2.61e-32 120
MS.gene07481.t1 MTR_1g095070 39.572 187 89 5 69 238 25 204 1.03e-31 116
MS.gene07481.t1 MTR_5g063930 34.538 249 136 7 13 249 28 261 2.45e-30 114
MS.gene07481.t1 MTR_3g008920 33.898 236 142 6 20 252 25 249 5.89e-29 110
MS.gene07481.t1 MTR_5g079570 32.627 236 142 5 20 251 39 261 5.10e-28 108
MS.gene07481.t1 MTR_2g017660 32.432 259 156 6 1 256 16 258 6.28e-27 105
MS.gene07481.t1 MTR_2g104940 34.404 218 135 3 25 242 84 293 1.34e-26 105
MS.gene07481.t1 MTR_2g017590 31.276 243 156 6 2 243 19 251 5.47e-26 102
MS.gene07481.t1 MTR_2g017620 33.333 228 139 5 15 239 18 235 1.22e-25 101
MS.gene07481.t1 MTR_2g017620 33.333 228 139 5 15 239 32 249 1.65e-25 101
MS.gene07481.t1 MTR_4g006730 34.404 218 135 3 25 242 79 288 1.90e-25 102
MS.gene07481.t1 MTR_4g084880 31.250 224 149 3 20 243 34 252 3.92e-25 100
MS.gene07481.t1 MTR_2g017610 31.535 241 147 6 10 243 24 253 1.35e-24 99.4
MS.gene07481.t1 MTR_8g087720 32.479 234 149 4 12 243 27 253 1.47e-24 99.4
MS.gene07481.t1 MTR_2g094270 35.377 212 123 5 11 209 30 240 1.48e-24 99.0
MS.gene07481.t1 MTR_2g017620 37.576 165 95 3 78 239 9 168 1.58e-24 97.1
MS.gene07481.t1 MTR_2g017570 31.799 239 145 6 12 243 26 253 1.23e-23 96.7
MS.gene07481.t1 MTR_8g087710 32.456 228 139 6 20 243 38 254 8.82e-23 94.7
MS.gene07481.t1 MTR_4g006630 33.493 209 131 3 34 242 1 201 1.32e-22 92.8
MS.gene07481.t1 MTR_4g006730 33.493 209 131 3 34 242 1 201 1.49e-22 92.8
MS.gene07481.t1 MTR_7g010650 29.218 243 145 7 21 257 37 258 1.29e-21 91.3
MS.gene07481.t1 MTR_3g070210 33.175 211 126 5 21 219 55 262 3.75e-21 90.1
MS.gene07481.t1 MTR_1g095070 35.152 165 83 5 69 216 25 182 7.14e-19 82.0
MS.gene07481.t1 MTR_1g070385 28.652 178 121 3 79 253 115 289 2.93e-18 84.3
MS.gene07481.t1 MTR_4g075570 34.078 179 88 7 79 234 104 275 1.37e-17 81.3
MS.gene07481.t1 MTR_4g075590 27.917 240 142 6 13 229 29 260 3.03e-17 80.1
MS.gene07481.t1 MTR_1g097840 30.357 224 145 5 25 245 78 293 3.38e-17 79.7
MS.gene07481.t1 MTR_8g098375 32.317 164 103 3 21 179 52 212 1.86e-16 76.6
MS.gene07481.t1 MTR_5g010150 31.928 166 101 4 21 179 52 212 2.66e-16 75.9
MS.gene07481.t1 MTR_4g059390 33.533 167 102 4 21 179 39 204 8.69e-16 73.9
MS.gene07481.t1 MTR_3g070210 31.902 163 103 3 21 178 55 214 8.11e-15 71.6
MS.gene07481.t1 MTR_4g415300 31.148 183 105 7 21 193 56 227 1.73e-14 70.9
MS.gene07481.t1 MTR_4g006730 33.533 167 103 3 25 191 79 237 5.50e-13 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07481.t1 AT1G73190 67.424 264 79 2 2 258 5 268 2.27e-120 344
MS.gene07481.t1 AT1G17810 68.182 264 76 3 2 258 5 267 1.81e-117 337
MS.gene07481.t1 AT4G01470 56.589 258 106 1 1 258 1 252 1.31e-100 294
MS.gene07481.t1 AT2G36830 53.876 258 112 1 1 258 1 251 1.02e-96 284
MS.gene07481.t1 AT3G26520 53.668 259 113 3 1 258 1 253 8.61e-94 276
MS.gene07481.t1 AT3G16240 49.603 252 121 1 7 258 5 250 6.33e-83 249
MS.gene07481.t1 AT5G47450 48.511 235 121 0 5 239 3 237 4.65e-76 231
MS.gene07481.t1 AT4G17340 48.387 248 128 0 5 252 3 250 1.00e-71 220
MS.gene07481.t1 AT2G25810 46.825 252 126 4 4 253 2 247 8.36e-67 207
MS.gene07481.t1 AT3G47440 43.612 227 123 3 18 242 20 243 5.72e-52 170
MS.gene07481.t1 AT2G37170 37.398 246 138 6 8 238 67 311 3.20e-39 139
MS.gene07481.t1 AT2G37170 37.398 246 138 6 8 238 24 268 3.26e-39 138
MS.gene07481.t1 AT2G37180 38.197 233 128 6 21 238 37 268 9.79e-38 134
MS.gene07481.t1 AT2G39010 37.657 239 121 7 21 238 38 269 3.18e-37 133
MS.gene07481.t1 AT3G53420 38.034 234 127 7 21 238 39 270 8.76e-37 132
MS.gene07481.t1 AT3G53420 38.034 234 127 7 21 238 39 270 8.76e-37 132
MS.gene07481.t1 AT2G16850 40.260 231 120 7 21 238 36 261 1.12e-36 131
MS.gene07481.t1 AT4G35100 38.589 241 130 7 11 238 28 263 4.11e-36 130
MS.gene07481.t1 AT4G35100 38.589 241 130 7 11 238 28 263 4.11e-36 130
MS.gene07481.t1 AT3G54820 36.752 234 130 7 21 238 38 269 6.06e-35 127
MS.gene07481.t1 AT5G60660 36.437 247 139 7 8 238 26 270 1.14e-33 124
MS.gene07481.t1 AT1G01620 38.095 231 125 7 21 238 52 277 1.35e-33 123
MS.gene07481.t1 AT4G00430 37.229 231 127 7 21 238 53 278 2.45e-33 122
MS.gene07481.t1 AT2G45960 37.083 240 124 9 21 243 52 281 3.97e-33 122
MS.gene07481.t1 AT3G61430 36.667 240 125 9 21 243 52 281 1.19e-32 120
MS.gene07481.t1 AT3G61430 36.667 240 125 9 21 243 52 281 1.19e-32 120
MS.gene07481.t1 AT4G23400 36.596 235 123 8 21 238 53 278 1.52e-32 120
MS.gene07481.t1 AT1G01620 41.436 181 94 4 69 238 26 205 3.02e-31 115
MS.gene07481.t1 AT5G37820 34.361 227 134 6 21 243 79 294 2.21e-28 110
MS.gene07481.t1 AT5G37820 34.361 227 134 6 21 243 43 258 2.36e-28 109
MS.gene07481.t1 AT5G37810 33.921 227 135 6 21 243 43 258 1.22e-25 102
MS.gene07481.t1 AT1G80760 33.784 222 139 4 21 242 80 293 7.29e-25 100
MS.gene07481.t1 AT1G52180 62.667 75 28 0 134 208 42 116 5.53e-24 94.0
MS.gene07481.t1 AT1G31885 35.135 222 130 6 20 234 42 256 1.12e-23 97.8
MS.gene07481.t1 AT4G19030 32.900 231 141 6 20 243 54 277 2.01e-23 96.7
MS.gene07481.t1 AT4G18910 31.169 231 145 6 20 243 51 274 1.57e-22 94.4
MS.gene07481.t1 AT2G45960 34.272 213 114 8 21 216 52 255 6.35e-22 92.4
MS.gene07481.t1 AT2G45960 34.272 213 114 8 21 216 52 255 1.02e-21 92.0
MS.gene07481.t1 AT1G31885 35.885 209 120 6 33 234 1 202 1.23e-21 91.3
MS.gene07481.t1 AT3G06100 26.337 243 168 4 3 243 28 261 1.26e-18 83.2
MS.gene07481.t1 AT4G10380 31.308 214 139 4 21 234 78 283 1.90e-18 83.2
MS.gene07481.t1 AT2G39010 33.140 172 94 5 21 178 38 202 1.94e-18 81.6
MS.gene07481.t1 AT3G06100 27.027 222 151 4 24 243 1 213 3.18e-16 75.9
MS.gene07481.t1 AT4G00430 33.728 169 101 5 21 183 53 216 1.26e-15 73.9
MS.gene07481.t1 AT2G34390 32.934 167 102 4 78 237 100 263 4.16e-15 73.6
MS.gene07481.t1 AT2G34390 32.934 167 102 4 78 237 58 221 6.84e-15 72.4
MS.gene07481.t1 AT3G06100 28.986 138 96 2 106 243 45 180 1.51e-11 62.4
MS.gene07481.t1 AT4G19030 31.250 176 107 6 20 188 54 222 9.97e-11 60.5

Find 71 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGGGTGGGTCCATTTCT+TGG 0.155301 1.2:+63225385 MS.gene07481:CDS
TTCAGCTACGGTTGCTCTTA+TGG 0.191582 1.2:-63222877 None:intergenic
GGGATTATCATTGGATCTTC+TGG 0.200631 1.2:+63225368 MS.gene07481:CDS
TGTGCCAATGGTGCCTCTTT+TGG 0.216016 1.2:-63225227 None:intergenic
TATTGGGTTGCTCAACTTCT+TGG 0.226985 1.2:+63223234 MS.gene07481:CDS
TAGTGACAAGCCTCAATAAA+AGG 0.227108 1.2:-63223272 None:intergenic
ACTTGCATCTTTGTCTTTGC+TGG 0.233185 1.2:+63222909 MS.gene07481:CDS
TCCTTAGAGCTGTCTACTAT+TGG 0.241545 1.2:+63223217 MS.gene07481:CDS
ATGAATCCTGCTAGGGCTTT+TGG 0.279098 1.2:+63225322 MS.gene07481:CDS
CCTTAGAGCTGTCTACTATT+GGG 0.314794 1.2:+63223218 MS.gene07481:CDS
ATCAACCCTGCAGTTACTTT+TGG 0.319696 1.2:+63223168 MS.gene07481:CDS
GAGGCTTGTCACTAATAATA+TGG 0.336955 1.2:+63223281 MS.gene07481:CDS
GTGGCATTTGGTGTTGGTGC+TGG 0.344660 1.2:+63225133 MS.gene07481:CDS
TCAACTTCTTGGTTCTTTAG+TGG 0.352258 1.2:+63223245 MS.gene07481:CDS
GAAAGTGACTAGTAATCTTC+AGG 0.355730 1.2:-63225493 None:intergenic
TCATCTAGCATGCATGTATC+TGG 0.363820 1.2:+63223138 MS.gene07481:CDS
TTAGTATGTGGAGGCTCAAC+TGG 0.363936 1.2:-63225448 None:intergenic
GGATTATCATTGGATCTTCT+GGG 0.375978 1.2:+63225369 MS.gene07481:CDS
CTGCTTTAGTGGGCTGGAGA+TGG 0.380286 1.2:+63225347 MS.gene07481:CDS
CCTTCCAAATGCATATCTTC+TGG 0.381858 1.2:-63222829 None:intergenic
ACGGTTGCTCTTATGGAATC+TGG 0.390261 1.2:-63222870 None:intergenic
ATCTTTGTCTTTGCTGGAGA+AGG 0.395798 1.2:+63222915 MS.gene07481:CDS
CTTGAGATTGCCATGACATT+TGG 0.396370 1.2:+63225169 MS.gene07481:CDS
TGCTTTAGTGGGCTGGAGAT+GGG 0.398103 1.2:+63225348 MS.gene07481:CDS
CAGGCGTTCAATGTGGCATT+TGG 0.406421 1.2:+63225121 MS.gene07481:CDS
CTAGCTGGTGGACCATTTGA+TGG 0.412946 1.2:+63225292 MS.gene07481:CDS
GCTATTGCAGCTCCAAGAAA+TGG 0.420934 1.2:-63225397 None:intergenic
TTAGCAATTGCACTTGTTGT+TGG 0.426628 1.2:+63225259 MS.gene07481:CDS
TTCAATGTGGCATTTGGTGT+TGG 0.431011 1.2:+63225127 MS.gene07481:CDS
TTGGACCTGCTTTAGTGGGC+TGG 0.433591 1.2:+63225341 MS.gene07481:CDS
CCCAATAGTAGACAGCTCTA+AGG 0.447069 1.2:-63223218 None:intergenic
TAATCCTCATCATCAACCTT+TGG 0.455773 1.2:+63225468 MS.gene07481:CDS
GCAACCAGAAGATATGCATT+TGG 0.457504 1.2:+63222825 MS.gene07481:CDS
AAGGATGCAGGTGCATCATC+TGG 0.462584 1.2:+63223063 MS.gene07481:CDS
AGTGACAAGCCTCAATAAAA+GGG 0.476287 1.2:-63223271 None:intergenic
TGGCATTTGGTGTTGGTGCT+GGG 0.481573 1.2:+63225134 MS.gene07481:CDS
TTCATGCATGCTCCATCAAA+TGG 0.481598 1.2:-63225304 None:intergenic
GCTGGAGATGGGATTATCAT+TGG 0.481888 1.2:+63225359 MS.gene07481:CDS
AACAACAAGTGCAATTGCTA+AGG 0.505583 1.2:-63225257 None:intergenic
CATCTCCAGCCCACTAAAGC+AGG 0.513626 1.2:-63225346 None:intergenic
GATCCCAAAAGAGGCACCAT+TGG 0.516327 1.2:+63225223 MS.gene07481:CDS
AGAGCACCAAAAGTAACTGC+AGG 0.519027 1.2:-63223174 None:intergenic
AAGATATGCATTTGGAAGGA+TGG 0.521944 1.2:+63222833 MS.gene07481:CDS
GCAGGTCCAAAAGCCCTAGC+AGG 0.531117 1.2:-63225328 None:intergenic
GAGCATGCATGAATCCTGCT+AGG 0.538388 1.2:+63225314 MS.gene07481:CDS
CATCAAATGGTCCACCAGCT+AGG 0.542081 1.2:-63225291 None:intergenic
ACTGCCATTGATCCCAAAAG+AGG 0.546787 1.2:+63225214 MS.gene07481:CDS
TATCATTGGATCTTCTGGGT+GGG 0.555235 1.2:+63225373 MS.gene07481:CDS
GGGAAGATATACAAGGATGC+AGG 0.559750 1.2:+63223051 MS.gene07481:intron
GTTGGTGCAAACATCCTAGC+TGG 0.564252 1.2:+63225277 MS.gene07481:CDS
TTATCATTGGATCTTCTGGG+TGG 0.568273 1.2:+63225372 MS.gene07481:CDS
TGCATCATCTGGTGAGCTAG+TGG 0.572276 1.2:+63223074 MS.gene07481:CDS
GGAGCCAAAGGTTGATGATG+AGG 0.575555 1.2:-63225472 None:intergenic
ATACATTAAACCAAATGTCA+TGG 0.580514 1.2:-63225179 None:intergenic
CCAGAAGATATGCATTTGGA+AGG 0.599791 1.2:+63222829 MS.gene07481:CDS
TAGTAATCTTCAGGAGCCAA+AGG 0.600955 1.2:-63225484 None:intergenic
TCTAGCATGCATGTATCTGG+TGG 0.608990 1.2:+63223141 MS.gene07481:CDS
AGCATGCATGAATCCTGCTA+GGG 0.613304 1.2:+63225315 MS.gene07481:CDS
TGCATTTGGAAGGATGGATG+AGG 0.613424 1.2:+63222839 MS.gene07481:CDS
TAAGGGTGCAATTGTGCCAA+TGG 0.613670 1.2:-63225239 None:intergenic
ACTACTACGAACTTAGAGCA+AGG 0.618291 1.2:-63222944 None:intergenic
ACAACAAGTGCAATTGCTAA+GGG 0.618371 1.2:-63225256 None:intergenic
TGATGATGAGGATTAGTATG+TGG 0.623112 1.2:-63225460 None:intergenic
TTGCCTGTTATAGAGACCAC+AGG 0.627295 1.2:+63225102 MS.gene07481:intron
CTACTACGAACTTAGAGCAA+GGG 0.634814 1.2:-63222943 None:intergenic
TAATGCAGGGAAGATATACA+AGG 0.641110 1.2:+63223044 MS.gene07481:intron
GAGCACCAAAAGTAACTGCA+GGG 0.663279 1.2:-63223173 None:intergenic
AATGCCACATTGAACGCCTG+TGG 0.670395 1.2:-63225118 None:intergenic
GAGACCACAGGCGTTCAATG+TGG 0.680641 1.2:+63225114 MS.gene07481:intron
GGTGCAAACATCCTAGCTGG+TGG 0.691436 1.2:+63225280 MS.gene07481:CDS
TGATGAGGATTAGTATGTGG+AGG 0.738837 1.2:-63225457 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCCATATAGATATATAATA+TGG - chr1.2:63222976-63222995 None:intergenic 15.0%
!! ATTAAAATAGAAGTGATTAT+TGG - chr1.2:63223378-63223397 None:intergenic 15.0%
!! CAAATAGATTAATGATATTT+GGG - chr1.2:63224916-63224935 None:intergenic 15.0%
!! TAATCATAAGTATATATAGA+AGG - chr1.2:63223004-63223023 None:intergenic 15.0%
!! TACCATATTATATATCTATA+TGG + chr1.2:63222971-63222990 MS.gene07481:intron 15.0%
!!! AAATCTAGAATCTAAAATTT+AGG + chr1.2:63224458-63224477 MS.gene07481:intron 15.0%
!!! AAATTGCATTTTTATTTTAC+CGG - chr1.2:63224381-63224400 None:intergenic 15.0%
!!! AATATTATGTTTTGTAGTTT+TGG + chr1.2:63224108-63224127 MS.gene07481:intron 15.0%
!!! ACTTTTATATGAATACATTT+TGG + chr1.2:63225423-63225442 MS.gene07481:CDS 15.0%
!!! ATATGTATTTTTTATTTGAG+TGG + chr1.2:63224791-63224810 MS.gene07481:intron 15.0%
!!! TATGTATTTTTTATTTGAGT+GGG + chr1.2:63224792-63224811 MS.gene07481:intron 15.0%
!! ACTATATAGTTAATTTAGAC+TGG + chr1.2:63224849-63224868 MS.gene07481:intron 20.0%
!! CCAAATAGATTAATGATATT+TGG - chr1.2:63224917-63224936 None:intergenic 20.0%
!! CCAAATATCATTAATCTATT+TGG + chr1.2:63224914-63224933 MS.gene07481:intron 20.0%
!! GTAAAAAATATGTTAGATGA+GGG - chr1.2:63223345-63223364 None:intergenic 20.0%
!! TAAAAAATATGTTAGATGAG+GGG - chr1.2:63223344-63223363 None:intergenic 20.0%
!! TAACAAAGCATATAAGATTT+CGG - chr1.2:63225040-63225059 None:intergenic 20.0%
!! TGTAAAAAATATGTTAGATG+AGG - chr1.2:63223346-63223365 None:intergenic 20.0%
!! TGTTAAATGTTGCAAATATA+TGG - chr1.2:63224574-63224593 None:intergenic 20.0%
!!! ATACTTATGATTAATTTGTG+TGG + chr1.2:63223011-63223030 MS.gene07481:intron 20.0%
!!! ATATAAGTTCTATTGATTAG+AGG + chr1.2:63223711-63223730 MS.gene07481:intron 20.0%
!!! ATGTATTTTTTATTTGAGTG+GGG + chr1.2:63224793-63224812 MS.gene07481:intron 20.0%
!!! ATTTACTGAACCATTTTTTT+TGG + chr1.2:63223989-63224008 MS.gene07481:intron 20.0%
!!! TAATGACTATTTTTGTATTG+TGG + chr1.2:63225079-63225098 MS.gene07481:intron 20.0%
!!! TATAAGTTCTATTGATTAGA+GGG + chr1.2:63223712-63223731 MS.gene07481:intron 20.0%
!!! TTAAACTTTTTAATCCTAAG+AGG + chr1.2:63224403-63224422 MS.gene07481:intron 20.0%
! AAAAATATTAAGCGAAAGAG+AGG + chr1.2:63224727-63224746 MS.gene07481:intron 25.0%
! ACAAGTATAAGCTCTATAAA+TGG - chr1.2:63223786-63223805 None:intergenic 25.0%
! ATACATCATCAAATTACATG+AGG - chr1.2:63224158-63224177 None:intergenic 25.0%
! ATACATTAAACCAAATGTCA+TGG - chr1.2:63225182-63225201 None:intergenic 25.0%
! TATGCAAAATACAATTCACT+AGG - chr1.2:63223501-63223520 None:intergenic 25.0%
!! TCTAGAATCTAAAATTTAGG+AGG + chr1.2:63224461-63224480 MS.gene07481:intron 25.0%
!!! TTATGATTAATTTGTGTGGT+TGG + chr1.2:63223015-63223034 MS.gene07481:intron 25.0%
AAATAGTCATTACGCATGAT+TGG - chr1.2:63225071-63225090 None:intergenic 30.0%
AAATTACATGAGGCCAATTA+TGG - chr1.2:63224148-63224167 None:intergenic 30.0%
AATTACATGAGGCCAATTAT+GGG - chr1.2:63224147-63224166 None:intergenic 30.0%
ACTTCGAGATGGATAAAAAT+AGG + chr1.2:63225009-63225028 MS.gene07481:intron 30.0%
AGTTGAGAAAAATTCAGCTA+CGG - chr1.2:63222892-63222911 None:intergenic 30.0%
ATTGAGCTCAAAACTCAAAA+GGG - chr1.2:63223421-63223440 None:intergenic 30.0%
CATAAAATATGTAAGGTCTG+GGG - chr1.2:63224033-63224052 None:intergenic 30.0%
CTTCGAGATGGATAAAAATA+GGG + chr1.2:63225010-63225029 MS.gene07481:intron 30.0%
GACGATGCATAAAATATGTA+AGG - chr1.2:63224040-63224059 None:intergenic 30.0%
GCATAAAATATGTAAGGTCT+GGG - chr1.2:63224034-63224053 None:intergenic 30.0%
TCTTCCTCTCATAGTAAAAT+AGG + chr1.2:63224352-63224371 MS.gene07481:intron 30.0%
TGCATAAAATATGTAAGGTC+TGG - chr1.2:63224035-63224054 None:intergenic 30.0%
TTACAATACCTAGTTAATCC+CGG + chr1.2:63224327-63224346 MS.gene07481:intron 30.0%
TTCGAGATGGATAAAAATAG+GGG + chr1.2:63225011-63225030 MS.gene07481:intron 30.0%
TTGAAAATTGTTGCATGTGT+AGG + chr1.2:63224872-63224891 MS.gene07481:intron 30.0%
! TATTGATTAGAGGGTTTCAA+AGG + chr1.2:63223721-63223740 MS.gene07481:intron 30.0%
!!! TACTGAACCATTTTTTTTGG+TGG + chr1.2:63223992-63224011 MS.gene07481:intron 30.0%
AACAACAAGTGCAATTGCTA+AGG - chr1.2:63225260-63225279 None:intergenic 35.0%
AAGAGACTAAAACTTCGAGA+TGG + chr1.2:63224998-63225017 MS.gene07481:intron 35.0%
AAGATATGCATTTGGAAGGA+TGG + chr1.2:63222833-63222852 MS.gene07481:CDS 35.0%
ACAACAAGTGCAATTGCTAA+GGG - chr1.2:63225259-63225278 None:intergenic 35.0%
AGTGACAAGCCTCAATAAAA+GGG - chr1.2:63223274-63223293 None:intergenic 35.0%
ATCCTAAGAGGCAATTTAAG+AGG + chr1.2:63224415-63224434 MS.gene07481:intron 35.0%
CTCATAGTAAAATAGGTCTC+CGG + chr1.2:63224359-63224378 MS.gene07481:intron 35.0%
GAGGCTTGTCACTAATAATA+TGG + chr1.2:63223281-63223300 MS.gene07481:CDS 35.0%
GATTGAGCTCAAAACTCAAA+AGG - chr1.2:63223422-63223441 None:intergenic 35.0%
GGATTATCATTGGATCTTCT+GGG + chr1.2:63225369-63225388 MS.gene07481:CDS 35.0%
TAATCCTCATCATCAACCTT+TGG + chr1.2:63225468-63225487 MS.gene07481:CDS 35.0%
TAATGCAGGGAAGATATACA+AGG + chr1.2:63223044-63223063 MS.gene07481:intron 35.0%
TACAATACCTAGTTAATCCC+GGG + chr1.2:63224328-63224347 MS.gene07481:intron 35.0%
TAGTGACAAGCCTCAATAAA+AGG - chr1.2:63223275-63223294 None:intergenic 35.0%
TTAGATGAGGGGAACAAATA+AGG - chr1.2:63223333-63223352 None:intergenic 35.0%
! AAATTTTGTCCTAGCTCAGT+TGG - chr1.2:63224067-63224086 None:intergenic 35.0%
! AGGCAATTTAAGAGGCATAA+AGG + chr1.2:63224423-63224442 MS.gene07481:intron 35.0%
! CTCTTATTTCCACCCATAAT+TGG + chr1.2:63224132-63224151 MS.gene07481:intron 35.0%
! GAGACCTATTTTACTATGAG+AGG - chr1.2:63224359-63224378 None:intergenic 35.0%
! TCAACTTCTTGGTTCTTTAG+TGG + chr1.2:63223245-63223264 MS.gene07481:CDS 35.0%
! TGATGATGAGGATTAGTATG+TGG - chr1.2:63225463-63225482 None:intergenic 35.0%
! TGGTTGGTTGAATTAATGCA+GGG + chr1.2:63223031-63223050 MS.gene07481:intron 35.0%
! TTGGATGATTTTACACCATC+TGG + chr1.2:63224933-63224952 MS.gene07481:intron 35.0%
!! TTAGCAATTGCACTTGTTGT+TGG + chr1.2:63225259-63225278 MS.gene07481:CDS 35.0%
!!! TGAACCATTTTTTTTGGTGG+TGG + chr1.2:63223995-63224014 MS.gene07481:intron 35.0%
ACTACTACGAACTTAGAGCA+AGG - chr1.2:63222947-63222966 None:intergenic 40.0%
ACTTGCATCTTTGTCTTTGC+TGG + chr1.2:63222909-63222928 MS.gene07481:CDS 40.0%
ATCTTTGTCTTTGCTGGAGA+AGG + chr1.2:63222915-63222934 MS.gene07481:CDS 40.0%
CCAGAAGATATGCATTTGGA+AGG + chr1.2:63222829-63222848 MS.gene07481:CDS 40.0%
CCTTAGAGCTGTCTACTATT+GGG + chr1.2:63223218-63223237 MS.gene07481:CDS 40.0%
CCTTCCAAATGCATATCTTC+TGG - chr1.2:63222832-63222851 None:intergenic 40.0%
CTACTACGAACTTAGAGCAA+GGG - chr1.2:63222946-63222965 None:intergenic 40.0%
CTTGAGATTGCCATGACATT+TGG + chr1.2:63225169-63225188 MS.gene07481:CDS 40.0%
GAGATGGATAAAAATAGGGG+AGG + chr1.2:63225014-63225033 MS.gene07481:intron 40.0%
GAGGGGAACAAATAAGGAAA+AGG - chr1.2:63223327-63223346 None:intergenic 40.0%
GCAACCAGAAGATATGCATT+TGG + chr1.2:63222825-63222844 MS.gene07481:CDS 40.0%
GGGAACAAATAAGGAAAAGG+TGG - chr1.2:63223324-63223343 None:intergenic 40.0%
GGGATTATCATTGGATCTTC+TGG + chr1.2:63225368-63225387 MS.gene07481:CDS 40.0%
TAGTAATCTTCAGGAGCCAA+AGG - chr1.2:63225487-63225506 None:intergenic 40.0%
TATCATTGGATCTTCTGGGT+GGG + chr1.2:63225373-63225392 MS.gene07481:CDS 40.0%
TCATCTAGCATGCATGTATC+TGG + chr1.2:63223138-63223157 MS.gene07481:CDS 40.0%
TCCTTAGAGCTGTCTACTAT+TGG + chr1.2:63223217-63223236 MS.gene07481:CDS 40.0%
TGCCTCTTAAATTGCCTCTT+AGG - chr1.2:63224420-63224439 None:intergenic 40.0%
TTACTATGAGAGGAAGAACC+CGG - chr1.2:63224349-63224368 None:intergenic 40.0%
TTATCATTGGATCTTCTGGG+TGG + chr1.2:63225372-63225391 MS.gene07481:CDS 40.0%
TTCATGCATGCTCCATCAAA+TGG - chr1.2:63225307-63225326 None:intergenic 40.0%
! GTGGTTGGTTGAATTAATGC+AGG + chr1.2:63223030-63223049 MS.gene07481:intron 40.0%
! TATTGGGTTGCTCAACTTCT+TGG + chr1.2:63223234-63223253 MS.gene07481:CDS 40.0%
! TGATGAGGATTAGTATGTGG+AGG - chr1.2:63225460-63225479 None:intergenic 40.0%
!! ATCAACCCTGCAGTTACTTT+TGG + chr1.2:63223168-63223187 MS.gene07481:CDS 40.0%
!! TTCAATGTGGCATTTGGTGT+TGG + chr1.2:63225127-63225146 MS.gene07481:CDS 40.0%
!!! GTTACTTTTGGTGCTCTTCT+TGG + chr1.2:63223180-63223199 MS.gene07481:CDS 40.0%
ACGGTTGCTCTTATGGAATC+TGG - chr1.2:63222873-63222892 None:intergenic 45.0%
ACTGCCATTGATCCCAAAAG+AGG + chr1.2:63225214-63225233 MS.gene07481:CDS 45.0%
AGCATGCATGAATCCTGCTA+GGG + chr1.2:63225315-63225334 MS.gene07481:CDS 45.0%
CCCAATAGTAGACAGCTCTA+AGG - chr1.2:63223221-63223240 None:intergenic 45.0%
GAGCACCAAAAGTAACTGCA+GGG - chr1.2:63223176-63223195 None:intergenic 45.0%
GCTATTGCAGCTCCAAGAAA+TGG - chr1.2:63225400-63225419 None:intergenic 45.0%
GCTGGAGATGGGATTATCAT+TGG + chr1.2:63225359-63225378 MS.gene07481:CDS 45.0%
GGGAAGATATACAAGGATGC+AGG + chr1.2:63223051-63223070 MS.gene07481:intron 45.0%
TACATGAGGCCAATTATGGG+TGG - chr1.2:63224144-63224163 None:intergenic 45.0%
TACTATGAGAGGAAGAACCC+GGG - chr1.2:63224348-63224367 None:intergenic 45.0%
TCGTCTATACCAACTGAGCT+AGG + chr1.2:63224055-63224074 MS.gene07481:intron 45.0%
TCTAGCATGCATGTATCTGG+TGG + chr1.2:63223141-63223160 MS.gene07481:CDS 45.0%
TGCATTTGGAAGGATGGATG+AGG + chr1.2:63222839-63222858 MS.gene07481:CDS 45.0%
TTAGTATGTGGAGGCTCAAC+TGG - chr1.2:63225451-63225470 None:intergenic 45.0%
TTCAGCTACGGTTGCTCTTA+TGG - chr1.2:63222880-63222899 None:intergenic 45.0%
TTGCCTGTTATAGAGACCAC+AGG + chr1.2:63225102-63225121 MS.gene07481:intron 45.0%
TTTCATGCGATACGACCAGA+TGG - chr1.2:63224951-63224970 None:intergenic 45.0%
! AGAGCACCAAAAGTAACTGC+AGG - chr1.2:63223177-63223196 None:intergenic 45.0%
! ATGAATCCTGCTAGGGCTTT+TGG + chr1.2:63225322-63225341 MS.gene07481:CDS 45.0%
! GCTTTTGGACCTGCTTTAGT+GGG + chr1.2:63225337-63225356 MS.gene07481:CDS 45.0%
! TAAGGGTGCAATTGTGCCAA+TGG - chr1.2:63225242-63225261 None:intergenic 45.0%
! TAGTGGCTGCCCTTTTATTG+AGG + chr1.2:63223262-63223281 MS.gene07481:CDS 45.0%
!!! ACTTTTGGTGCTCTTCTTGG+TGG + chr1.2:63223183-63223202 MS.gene07481:CDS 45.0%
!!! ATTTTTTTTGGTGGTGGCCG+GGG + chr1.2:63224001-63224020 MS.gene07481:intron 45.0%
!!! CATTTTTTTTGGTGGTGGCC+GGG + chr1.2:63224000-63224019 MS.gene07481:intron 45.0%
!!! CCATTTTTTTTGGTGGTGGC+CGG + chr1.2:63223999-63224018 MS.gene07481:intron 45.0%
!!! ATTTTATTTTGAAATAAAAT+TGG + chr1.2:63223451-63223470 MS.gene07481:intron 5.0%
!!! TTTTATTTTGAAATAAAATT+GGG + chr1.2:63223452-63223471 MS.gene07481:intron 5.0%
AAGGATGCAGGTGCATCATC+TGG + chr1.2:63223063-63223082 MS.gene07481:CDS 50.0%
AATGCCACATTGAACGCCTG+TGG - chr1.2:63225121-63225140 None:intergenic 50.0%
ACGCCTGTGGTCTCTATAAC+AGG - chr1.2:63225108-63225127 None:intergenic 50.0%
CAGGCGTTCAATGTGGCATT+TGG + chr1.2:63225121-63225140 MS.gene07481:CDS 50.0%
CATCAAATGGTCCACCAGCT+AGG - chr1.2:63225294-63225313 None:intergenic 50.0%
CCGGCCACCACCAAAAAAAA+TGG - chr1.2:63224002-63224021 None:intergenic 50.0%
GAGCATGCATGAATCCTGCT+AGG + chr1.2:63225314-63225333 MS.gene07481:CDS 50.0%
GAGTGGGGTGTATTCACATC+TGG + chr1.2:63224808-63224827 MS.gene07481:intron 50.0%
GGAAGAACCCGGGATTAACT+AGG - chr1.2:63224338-63224357 None:intergenic 50.0%
GTTGGTGCAAACATCCTAGC+TGG + chr1.2:63225277-63225296 MS.gene07481:CDS 50.0%
TGCATCATCTGGTGAGCTAG+TGG + chr1.2:63223074-63223093 MS.gene07481:CDS 50.0%
TTCTGGGTGGGTCCATTTCT+TGG + chr1.2:63225385-63225404 MS.gene07481:CDS 50.0%
! CTAGCTGGTGGACCATTTGA+TGG + chr1.2:63225292-63225311 MS.gene07481:CDS 50.0%
! GGCTTTTGGACCTGCTTTAG+TGG + chr1.2:63225336-63225355 MS.gene07481:CDS 50.0%
! GTGCCAATGGTGCCTCTTTT+GGG - chr1.2:63225229-63225248 None:intergenic 50.0%
! TGTGCCAATGGTGCCTCTTT+TGG - chr1.2:63225230-63225249 None:intergenic 50.0%
!! GATCCCAAAAGAGGCACCAT+TGG + chr1.2:63225223-63225242 MS.gene07481:CDS 50.0%
!! GGAGCCAAAGGTTGATGATG+AGG - chr1.2:63225475-63225494 None:intergenic 50.0%
!! TGCTTTAGTGGGCTGGAGAT+GGG + chr1.2:63225348-63225367 MS.gene07481:CDS 50.0%
!! TGGCATTTGGTGTTGGTGCT+GGG + chr1.2:63225134-63225153 MS.gene07481:CDS 50.0%
!!! GGTGCCTCTTTTGGGATCAA+TGG - chr1.2:63225221-63225240 None:intergenic 50.0%
CATCTCCAGCCCACTAAAGC+AGG - chr1.2:63225349-63225368 None:intergenic 55.0%
GAGACCACAGGCGTTCAATG+TGG + chr1.2:63225114-63225133 MS.gene07481:intron 55.0%
GGTGCAAACATCCTAGCTGG+TGG + chr1.2:63225280-63225299 MS.gene07481:CDS 55.0%
! TTGGACCTGCTTTAGTGGGC+TGG + chr1.2:63225341-63225360 MS.gene07481:CDS 55.0%
!! CTGCTTTAGTGGGCTGGAGA+TGG + chr1.2:63225347-63225366 MS.gene07481:CDS 55.0%
!! GTGGCATTTGGTGTTGGTGC+TGG + chr1.2:63225133-63225152 MS.gene07481:CDS 55.0%
GCAGGTCCAAAAGCCCTAGC+AGG - chr1.2:63225331-63225350 None:intergenic 60.0%
! AAGGTCTGGGGTTCGAACCC+CGG - chr1.2:63224021-63224040 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 63222822 63225507 63222822 ID=MS.gene07481
chr1.2 mRNA 63222822 63225507 63222822 ID=MS.gene07481.t1;Parent=MS.gene07481
chr1.2 exon 63222822 63222954 63222822 ID=MS.gene07481.t1.exon1;Parent=MS.gene07481.t1
chr1.2 CDS 63222822 63222954 63222822 ID=cds.MS.gene07481.t1;Parent=MS.gene07481.t1
chr1.2 exon 63223052 63223302 63223052 ID=MS.gene07481.t1.exon2;Parent=MS.gene07481.t1
chr1.2 CDS 63223052 63223302 63223052 ID=cds.MS.gene07481.t1;Parent=MS.gene07481.t1
chr1.2 exon 63225115 63225507 63225115 ID=MS.gene07481.t1.exon3;Parent=MS.gene07481.t1
chr1.2 CDS 63225115 63225507 63225115 ID=cds.MS.gene07481.t1;Parent=MS.gene07481.t1
Gene Sequence

>MS.gene07481

ATGGCAACCAGAAGATATGCATTTGGAAGGATGGATGAGGCAAATCATCCAGATTCCATAAGAGCAACCGTAGCTGAATTTTTCTCAACTTGCATCTTTGTCTTTGCTGGAGAAGGATCTGCCCTTGCTCTAAGTTCGTAGTAGTGTAGTACCATATTATATATCTATATGGATACACTCCTTCTATATATACTTATGATTAATTTGTGTGGTTGGTTGAATTAATGCAGGGAAGATATACAAGGATGCAGGTGCATCATCTGGTGAGCTAGTGGTACTAGCACTAGCTCATGCATTTTCACTATTTGCAGCTATATCATCTAGCATGCATGTATCTGGTGGACATATCAACCCTGCAGTTACTTTTGGTGCTCTTCTTGGTGGTAGAATCTCTGTCCTTAGAGCTGTCTACTATTGGGTTGCTCAACTTCTTGGTTCTTTAGTGGCTGCCCTTTTATTGAGGCTTGTCACTAATAATATGGTATAACATTACTCAATTCCACCTTTTCCTTATTTGTTCCCCTCATCTAACATATTTTTTACATTATTTTATCCAATAATCACTTCTATTTTAATCTTAATCAGATAGACTTTAACCCTTTTGAGTTTTGAGCTCAATCACTTGTTTTATTTTATTTTGAAATAAAATTGGGAAGAGAAAATTACTCATCACTTTCCTAGTGAATTGTATTTTGCATAGATTAAGAAAAACAAATAGAAGAGTTAAAACAATAAGTTTACTAAATTACTCTTATTGATATATATTATTATACGACAAATGTAAAATGAAAGATATTCACTTGAGATAGAGCTTATAAATGTAAAATGAAAGATATTCACTTGAGATAATGAAAGATATTCACTTGAGATAGAGCTTATACTTGTACGAATATAAGTTCTATTGATTAGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAATTTTAAAATTTACTGAACCATTTATAGAGCTTATACTTGTACTAATATAAGTTCTATTGATTAGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAATTTCAAAATTTACTGAACCATTTATAGAGCTTATACTTGTACGAATATAAGTTCTATTGATTAGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAATTTCAAAAAAAGTCAAAATTTCAAAATTTACTGAACCATTTTTTTTGGTGGTGGCCGGGGTTCGAACCCCAGACCTTACATATTTTATGCATCGTCTATACCAACTGAGCTAGGACAAAATTTACTGAACAATTATCACTTATTAATATTATGTTTTGTAGTTTTGGTCTCTTATTTCCACCCATAATTGGCCTCATGTAATTTGATGATGTATAGTGATCAACACTTGTTTTCTTAGTTTTTTACTTAACACGATCAACACTTACAATTTCAACTTTAAAAATTAATTATTTTATAATTTGATTATCGATATACAAATAACTAATCTACAAATGTTTTATGAAAACTCAATCTTCTCTACACTTACAATACCTAGTTAATCCCGGGTTCTTCCTCTCATAGTAAAATAGGTCTCCGGTAAAATAAAAATGCAATTTTTTTAAACTTTTTAATCCTAAGAGGCAATTTAAGAGGCATAAAGGAAGACTAAAAAAAAATCTAGAATCTAAAATTTAGGAGGCATATATTAGCAATATTCTCAACATTCAACGTCAATCATATAATGAAATTAGCAATTATATTTTTCAAACTTCAACATATATTAGAACCATATATTTGCAACATTTAACAAAATTAGCAATTATACACTGAAATTTTAAAAAGTAATTACTAATAAACATTAAGCAACGTATAACAAAATTCGTGTAGCTAATTTTCATTAAAAAATCATAATTAGAAGAACTTCTAAATATGAAACGAAATAAAAAATATTAAGCGAAAGAGAGGTGTAGAGTGAAACGCGTATTGAATAAAGAAAAAGAATGAGCATATGTATTTTTTATTTGAGTGGGGTGTATTCACATCTGGTCAATTCACATGCACTTAACTATATAGTTAATTTAGACTGGTTGAAAATTGTTGCATGTGTAGGAAAAAAAAAAAAAGAGTACCCAAATATCATTAATCTATTTGGATGATTTTACACCATCTGGTCGTATCGCATGAAACGTCATATTTTAGTTGACGAATTGAATAAGAGACTAAAACTTCGAGATGGATAAAAATAGGGGAGGCCGAAATCTTATATGCTTTGTTATTCTCATGCCAATCATGCGTAATGACTATTTTTGTATTGTGGTTGCCTGTTATAGAGACCACAGGCGTTCAATGTGGCATTTGGTGTTGGTGCTGGGAACGCTCTTGTACTTGAGATTGCCATGACATTTGGTTTAATGTATGTTGTGTATGCAACTGCCATTGATCCCAAAAGAGGCACCATTGGCACAATTGCACCCTTAGCAATTGCACTTGTTGTTGGTGCAAACATCCTAGCTGGTGGACCATTTGATGGAGCATGCATGAATCCTGCTAGGGCTTTTGGACCTGCTTTAGTGGGCTGGAGATGGGATTATCATTGGATCTTCTGGGTGGGTCCATTTCTTGGAGCTGCAATAGCAGCACTTTTATATGAATACATTTTGGTTCCAGTTGAGCCTCCACATACTAATCCTCATCATCAACCTTTGGCTCCTGAAGATTACTAG
Protein sequence

>MS.gene07481.t1

MATRRYAFGRMDEANHPDSIRATVAEFFSTCIFVFAGEGSALALRKIYKDAGASSGELVVLALAHAFSLFAAISSSMHVSGGHINPAVTFGALLGGRISVLRAVYYWVAQLLGSLVAALLLRLVTNNMRPQAFNVAFGVGAGNALVLEIAMTFGLMYVVYATAIDPKRGTIGTIAPLAIALVVGANILAGGPFDGACMNPARAFGPALVGWRWDYHWIFWVGPFLGAAIAALLYEYILVPVEPPHTNPHHQPLAPEDY