AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004953

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004953.t1 MTR_1g077540 97.287 258 7 0 1 258 1 258 0.0 505
MS.gene004953.t1 MTR_6g069040 79.070 258 51 1 1 258 1 255 7.50e-143 400
MS.gene004953.t1 MTR_1g115405 53.488 258 114 1 1 258 1 252 1.45e-94 278
MS.gene004953.t1 MTR_4g063090 53.101 258 115 1 1 258 1 252 4.28e-92 272
MS.gene004953.t1 MTR_7g023350 53.488 258 114 1 1 258 1 252 7.55e-92 271
MS.gene004953.t1 MTR_2g101370 51.779 253 115 1 4 256 3 248 1.79e-88 263
MS.gene004953.t1 MTR_7g103030 53.488 258 112 2 1 258 1 250 3.12e-87 259
MS.gene004953.t1 MTR_8g013680 49.600 250 119 1 7 256 5 247 3.07e-83 249
MS.gene004953.t1 MTR_5g012810 50.833 240 117 1 5 244 3 241 1.63e-72 222
MS.gene004953.t1 MTR_1g006490 44.664 253 129 3 5 257 3 244 4.98e-71 218
MS.gene004953.t1 MTR_1g006590 44.269 253 130 3 5 257 3 244 1.02e-66 207
MS.gene004953.t1 MTR_2g060360 39.738 229 137 1 10 238 8 235 1.92e-51 168
MS.gene004953.t1 MTR_2g094270 36.475 244 138 6 11 238 30 272 1.21e-35 129
MS.gene004953.t1 MTR_3g118010 37.555 229 129 5 21 238 37 262 1.23e-34 125
MS.gene004953.t1 MTR_7g101190 36.481 233 132 6 21 238 39 270 5.22e-34 124
MS.gene004953.t1 MTR_4g059390 36.910 233 131 6 21 238 39 270 3.04e-33 122
MS.gene004953.t1 MTR_8g098375 35.193 233 136 5 21 241 52 281 6.37e-33 121
MS.gene004953.t1 MTR_5g010150 34.894 235 134 6 21 241 52 281 6.57e-33 121
MS.gene004953.t1 MTR_3g070210 34.310 239 142 5 21 247 55 290 4.61e-31 117
MS.gene004953.t1 MTR_5g082070 35.319 235 136 6 21 243 55 285 6.25e-31 116
MS.gene004953.t1 MTR_4g415300 35.043 234 134 7 21 241 56 284 6.87e-31 116
MS.gene004953.t1 MTR_1g095070 38.503 187 91 5 69 238 25 204 2.60e-30 113
MS.gene004953.t1 MTR_5g063930 34.008 247 137 7 13 249 28 258 7.73e-30 112
MS.gene004953.t1 MTR_3g008920 33.475 236 143 6 20 252 25 249 1.09e-28 110
MS.gene004953.t1 MTR_5g079570 32.203 236 143 5 20 251 39 261 1.02e-27 107
MS.gene004953.t1 MTR_2g017660 32.114 246 157 4 1 243 16 254 1.56e-26 104
MS.gene004953.t1 MTR_2g017590 30.864 243 157 6 2 243 19 251 5.82e-26 102
MS.gene004953.t1 MTR_2g017620 32.751 229 141 5 15 240 18 236 1.51e-25 101
MS.gene004953.t1 MTR_2g017620 32.751 229 141 5 15 240 32 250 2.37e-25 101
MS.gene004953.t1 MTR_4g084880 30.804 224 150 3 20 243 34 252 6.06e-25 100
MS.gene004953.t1 MTR_2g104940 33.641 217 136 3 26 242 85 293 6.53e-25 100
MS.gene004953.t1 MTR_2g017620 37.195 164 99 3 78 240 9 169 6.79e-25 98.2
MS.gene004953.t1 MTR_2g017610 31.120 241 148 6 10 243 24 253 1.78e-24 99.0
MS.gene004953.t1 MTR_8g087720 32.051 234 150 4 12 243 27 253 1.97e-24 99.0
MS.gene004953.t1 MTR_2g094270 34.906 212 124 5 11 209 30 240 6.23e-24 97.4
MS.gene004953.t1 MTR_4g006730 33.641 217 136 3 26 242 80 288 8.79e-24 97.8
MS.gene004953.t1 MTR_2g017570 37.126 167 101 3 78 243 90 253 1.26e-23 96.7
MS.gene004953.t1 MTR_8g087710 32.018 228 140 6 20 243 38 254 8.38e-23 94.7
MS.gene004953.t1 MTR_4g006730 34.021 194 120 3 34 227 1 186 1.54e-21 90.1
MS.gene004953.t1 MTR_4g006630 34.021 194 120 3 34 227 1 186 1.75e-21 89.7
MS.gene004953.t1 MTR_7g010650 29.412 238 141 7 26 257 42 258 5.00e-21 89.7
MS.gene004953.t1 MTR_3g070210 32.692 208 123 5 21 214 55 259 6.10e-20 87.0
MS.gene004953.t1 MTR_1g070385 28.652 178 121 3 79 253 115 289 2.80e-18 84.3
MS.gene004953.t1 MTR_1g095070 33.721 172 88 5 69 221 25 189 5.23e-18 79.7
MS.gene004953.t1 MTR_4g075570 28.571 252 145 9 13 241 43 282 1.16e-17 81.3
MS.gene004953.t1 MTR_4g075590 27.778 252 147 7 13 241 29 268 2.69e-17 80.1
MS.gene004953.t1 MTR_1g097840 30.045 223 145 5 26 245 79 293 4.69e-17 79.3
MS.gene004953.t1 MTR_8g098375 31.707 164 104 3 21 179 52 212 9.64e-16 74.7
MS.gene004953.t1 MTR_5g010150 31.325 166 102 4 21 179 52 212 1.05e-15 74.3
MS.gene004953.t1 MTR_4g059390 32.934 167 103 4 21 179 39 204 3.52e-15 72.4
MS.gene004953.t1 MTR_3g070210 31.288 163 104 3 21 178 55 214 3.89e-14 69.7
MS.gene004953.t1 MTR_4g415300 30.178 169 99 6 21 179 56 215 7.88e-14 69.3
MS.gene004953.t1 MTR_4g006730 33.133 166 103 3 26 191 80 237 2.36e-12 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004953.t1 AT1G73190 67.045 264 80 2 2 258 5 268 4.93e-120 343
MS.gene004953.t1 AT1G17810 67.424 264 78 3 2 258 5 267 1.28e-115 332
MS.gene004953.t1 AT4G01470 56.202 258 107 1 1 258 1 252 4.04e-100 292
MS.gene004953.t1 AT2G36830 53.488 258 113 1 1 258 1 251 4.60e-96 282
MS.gene004953.t1 AT3G26520 53.282 259 114 3 1 258 1 253 4.43e-93 275
MS.gene004953.t1 AT3G16240 49.206 252 122 1 7 258 5 250 1.56e-82 248
MS.gene004953.t1 AT5G47450 48.511 235 121 0 5 239 3 237 8.84e-76 231
MS.gene004953.t1 AT4G17340 48.387 248 128 0 5 252 3 250 1.83e-71 219
MS.gene004953.t1 AT2G25810 46.825 252 126 4 4 253 2 247 3.87e-67 208
MS.gene004953.t1 AT3G47440 43.172 227 124 3 18 242 20 243 2.90e-50 166
MS.gene004953.t1 AT2G37170 36.179 246 141 6 8 238 24 268 2.92e-37 133
MS.gene004953.t1 AT2G37170 36.179 246 141 6 8 238 67 311 3.67e-37 134
MS.gene004953.t1 AT2G37180 36.910 233 131 6 21 238 37 268 8.01e-36 129
MS.gene004953.t1 AT2G39010 36.402 239 124 7 21 238 38 269 3.36e-35 127
MS.gene004953.t1 AT2G16850 38.961 231 123 7 21 238 36 261 7.80e-35 126
MS.gene004953.t1 AT3G53420 36.752 234 130 7 21 238 39 270 7.82e-35 126
MS.gene004953.t1 AT3G53420 36.752 234 130 7 21 238 39 270 7.82e-35 126
MS.gene004953.t1 AT4G35100 37.344 241 133 7 11 238 28 263 4.30e-34 124
MS.gene004953.t1 AT4G35100 37.344 241 133 7 11 238 28 263 4.30e-34 124
MS.gene004953.t1 AT3G54820 35.470 234 133 7 21 238 38 269 5.17e-33 122
MS.gene004953.t1 AT1G01620 36.752 234 130 7 21 241 52 280 5.31e-32 119
MS.gene004953.t1 AT5G60660 35.223 247 142 7 8 238 26 270 8.09e-32 119
MS.gene004953.t1 AT4G00430 35.897 234 132 7 21 241 53 281 1.33e-31 118
MS.gene004953.t1 AT2G45960 36.250 240 126 9 21 243 52 281 1.43e-31 118
MS.gene004953.t1 AT3G61430 35.833 240 127 9 21 243 52 281 4.54e-31 117
MS.gene004953.t1 AT3G61430 35.833 240 127 9 21 243 52 281 4.54e-31 117
MS.gene004953.t1 AT4G23400 35.294 238 128 8 21 241 53 281 6.83e-31 116
MS.gene004953.t1 AT1G01620 40.217 184 98 4 69 241 26 208 3.19e-30 112
MS.gene004953.t1 AT5G37820 38.857 175 97 4 73 243 126 294 2.02e-28 110
MS.gene004953.t1 AT5G37820 38.857 175 97 4 73 243 90 258 2.04e-28 109
MS.gene004953.t1 AT5G37810 37.714 175 99 4 73 243 90 258 1.88e-25 102
MS.gene004953.t1 AT1G52180 62.667 75 28 0 134 208 42 116 5.19e-24 94.0
MS.gene004953.t1 AT1G80760 33.180 217 137 4 26 242 85 293 1.68e-23 97.1
MS.gene004953.t1 AT4G19030 32.468 231 142 6 20 243 54 277 2.93e-23 96.3
MS.gene004953.t1 AT1G31885 35.135 222 130 6 20 234 42 256 3.08e-23 96.7
MS.gene004953.t1 AT4G18910 30.736 231 146 6 20 243 51 274 1.97e-22 94.0
MS.gene004953.t1 AT1G31885 36.058 208 119 6 34 234 2 202 9.26e-22 91.7
MS.gene004953.t1 AT2G45960 33.990 203 111 7 21 209 52 245 1.97e-20 88.2
MS.gene004953.t1 AT2G45960 33.990 203 111 7 21 209 52 245 2.34e-20 88.6
MS.gene004953.t1 AT4G10380 30.045 223 145 5 26 245 83 297 1.43e-18 83.6
MS.gene004953.t1 AT3G06100 25.926 243 169 4 3 243 28 261 6.25e-18 81.3
MS.gene004953.t1 AT2G39010 32.558 172 95 5 21 178 38 202 9.95e-18 79.7
MS.gene004953.t1 AT3G06100 28.022 182 129 2 62 243 34 213 4.18e-16 75.5
MS.gene004953.t1 AT2G34390 32.934 167 102 4 78 237 100 263 4.82e-15 73.6
MS.gene004953.t1 AT4G00430 33.136 169 102 5 21 183 53 216 5.88e-15 72.0
MS.gene004953.t1 AT2G34390 32.934 167 102 4 78 237 58 221 7.41e-15 72.4
MS.gene004953.t1 AT3G06100 28.986 138 96 2 106 243 45 180 1.60e-11 62.4

Find 73 sgRNAs with CRISPR-Local

Find 167 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCTGGGTGGGTCCATTTCT+TGG 0.155301 1.4:-63551682 MS.gene004953:CDS
GGCATTATCATTGGATCTTC+TGG 0.179466 1.4:-63551699 MS.gene004953:CDS
TTCAACTACGGTTGCTCTTA+TGG 0.185278 1.4:+63554340 None:intergenic
TGTGCCAATGGTGCCTCTTT+TGG 0.216016 1.4:+63551840 None:intergenic
TATTGGGTTGCTCAACTTCT+TGG 0.226985 1.4:-63553983 MS.gene004953:CDS
TAGTGACAAGCCTCAATAAA+AGG 0.227108 1.4:+63553945 None:intergenic
ACTTGCATCTTTGTCTTTGC+TGG 0.233185 1.4:-63554308 MS.gene004953:CDS
TCCTTAGAGCTGTCTACTAT+TGG 0.241545 1.4:-63554000 MS.gene004953:CDS
GTTACCTTTGGTGCTCTTCT+TGG 0.247119 1.4:-63554037 MS.gene004953:CDS
ATGAACCCTGCTAGGGCTTT+TGG 0.293034 1.4:-63551745 MS.gene004953:CDS
CCTTAGAGCTGTCTACTATT+GGG 0.314794 1.4:-63553999 MS.gene004953:CDS
GAGGCTTGTCACTAATAATA+TGG 0.336955 1.4:-63553936 MS.gene004953:intron
GTGGCATTTGGTGTTGGTGC+TGG 0.344660 1.4:-63551934 MS.gene004953:CDS
TCAACTTCTTGGTTCTTTAG+TGG 0.352258 1.4:-63553972 MS.gene004953:CDS
GAAAGTGACTAGTAATCTTC+AGG 0.355730 1.4:+63551574 None:intergenic
TCATCTAGCATGCATGTATC+TGG 0.363820 1.4:-63554079 MS.gene004953:CDS
CCTTCCAAATGCATATCTTC+TGG 0.381858 1.4:+63554388 None:intergenic
GCATTATCATTGGATCTTCT+GGG 0.387836 1.4:-63551698 MS.gene004953:CDS
ATCTTTGTCTTTGCTGGAGA+AGG 0.395798 1.4:-63554302 MS.gene004953:CDS
ACGGTTGCTCTTATGGAATC+TGG 0.398639 1.4:+63554347 None:intergenic
CTAGCTGGTGGACCGTTTGA+TGG 0.398686 1.4:-63551775 MS.gene004953:CDS
ATCAACCCTGCTGTTACCTT+TGG 0.403209 1.4:-63554049 MS.gene004953:CDS
CAGGCGTTCAATGTGGCATT+TGG 0.406421 1.4:-63551946 MS.gene004953:CDS
CTTGAGATTGCCATGACGTT+TGG 0.407861 1.4:-63551898 MS.gene004953:CDS
TTAGCAATTGCACTTGTAGT+TGG 0.417528 1.4:-63551808 MS.gene004953:CDS
GCTATTGCAGCTCCAAGAAA+TGG 0.420934 1.4:+63551670 None:intergenic
GTTGGAGATGGCATTATCAT+TGG 0.427808 1.4:-63551708 MS.gene004953:CDS
TTCAATGTGGCATTTGGTGT+TGG 0.431011 1.4:-63551940 MS.gene004953:CDS
TTGGACCTGCTTTAGTGGGT+TGG 0.436140 1.4:-63551726 MS.gene004953:CDS
CCCAATAGTAGACAGCTCTA+AGG 0.447069 1.4:+63553999 None:intergenic
TAATCCTCATCATCAACCTT+TGG 0.455773 1.4:-63551599 MS.gene004953:CDS
CTGCTTTAGTGGGTTGGAGA+TGG 0.457448 1.4:-63551720 MS.gene004953:CDS
GCAACCAGAAGATATGCATT+TGG 0.457504 1.4:-63554392 MS.gene004953:CDS
TGATGATGAGGATTAGTATG+AGG 0.461315 1.4:+63551607 None:intergenic
AAGGATGCAGGTGCATCATC+TGG 0.462584 1.4:-63554154 MS.gene004953:CDS
ACCTTTGGTGCTCTTCTTGG+TGG 0.475601 1.4:-63554034 MS.gene004953:CDS
AGTGACAAGCCTCAATAAAA+GGG 0.476287 1.4:+63553946 None:intergenic
GCAGGTCCAAAAGCCCTAGC+AGG 0.476305 1.4:+63551739 None:intergenic
AGAGCACCAAAGGTAACAGC+AGG 0.480841 1.4:+63554043 None:intergenic
TGGCATTTGGTGTTGGTGCT+GGG 0.481573 1.4:-63551933 MS.gene004953:CDS
AACTACAAGTGCAATTGCTA+AGG 0.484188 1.4:+63551810 None:intergenic
GAGCATGCATGAACCCTGCT+AGG 0.512331 1.4:-63551753 MS.gene004953:CDS
TTCATGCATGCTCCATCAAA+CGG 0.513236 1.4:+63551763 None:intergenic
GATCCCAAAAGAGGCACCAT+TGG 0.516327 1.4:-63551844 MS.gene004953:CDS
AAGATATGCATTTGGAAGGA+TGG 0.521944 1.4:-63554384 MS.gene004953:CDS
CATCAAACGGTCCACCAGCT+AGG 0.543543 1.4:+63551776 None:intergenic
ACTGCCATTGATCCCAAAAG+AGG 0.546787 1.4:-63551853 MS.gene004953:CDS
GTTGGAGCAAACATCCTAGC+TGG 0.554931 1.4:-63551790 MS.gene004953:CDS
CATCTCCAACCCACTAAAGC+AGG 0.558301 1.4:+63551721 None:intergenic
GGGAAGATATACAAGGATGC+AGG 0.559750 1.4:-63554166 MS.gene004953:CDS
TATCATTGGATCTTCTGGGT+GGG 0.567093 1.4:-63551694 MS.gene004953:CDS
TGCATCATCTGGTGAGCTAG+TGG 0.572276 1.4:-63554143 MS.gene004953:CDS
GGAGCCAAAGGTTGATGATG+AGG 0.575555 1.4:+63551595 None:intergenic
TTATCATTGGATCTTCTGGG+TGG 0.594315 1.4:-63551695 MS.gene004953:CDS
ACCACCAAGAAGAGCACCAA+AGG 0.595805 1.4:+63554033 None:intergenic
AGCATGCATGAACCCTGCTA+GGG 0.596030 1.4:-63551752 MS.gene004953:CDS
CCAGAAGATATGCATTTGGA+AGG 0.599791 1.4:-63554388 MS.gene004953:CDS
TAGTAATCTTCAGGAGCCAA+AGG 0.600955 1.4:+63551583 None:intergenic
ACTACAAGTGCAATTGCTAA+GGG 0.608280 1.4:+63551811 None:intergenic
TCTAGCATGCATGTATCTGG+TGG 0.608990 1.4:-63554076 MS.gene004953:CDS
CAGGTCCAAAAGCCCTAGCA+GGG 0.610940 1.4:+63551740 None:intergenic
TGCATTTGGAAGGATGGATG+AGG 0.613424 1.4:-63554378 MS.gene004953:CDS
TAAGGGTGCAATTGTGCCAA+TGG 0.613670 1.4:+63551828 None:intergenic
ACTACTACGAACTTAGAGCA+AGG 0.618291 1.4:+63554273 None:intergenic
ATACATTAAGCCAAACGTCA+TGG 0.620799 1.4:+63551888 None:intergenic
GGAGCAAACATCCTAGCTGG+TGG 0.624019 1.4:-63551787 MS.gene004953:CDS
TTGCCTGTTATAGAGACCAC+AGG 0.627295 1.4:-63551965 MS.gene004953:intron
CTACTACGAACTTAGAGCAA+GGG 0.634814 1.4:+63554274 None:intergenic
TAATGCAGGGAAGATATACA+AGG 0.641110 1.4:-63554173 MS.gene004953:intron
TGATGAGGATTAGTATGAGG+AGG 0.643456 1.4:+63551610 None:intergenic
AATGCCACATTGAACGCCTG+TGG 0.670395 1.4:+63551949 None:intergenic
GAGACCACAGGCGTTCAATG+TGG 0.680641 1.4:-63551953 MS.gene004953:CDS
GAGCACCAAAGGTAACAGCA+GGG 0.691088 1.4:+63554044 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ACTTTTATATGAATATATTA+TGG - chr1.4:63554333-63554352 MS.gene004953:CDS 10.0%
!! ATCCATATAGATATATAATA+TGG + chr1.4:63551736-63551755 None:intergenic 15.0%
!! CAAATAGATTAATGATATTT+GGG + chr1.4:63553826-63553845 None:intergenic 15.0%
!! TACCATATTATATATCTATA+TGG - chr1.4:63551731-63551750 MS.gene004953:CDS 15.0%
!!! AAATCTAGAATCTAAAATTT+AGG - chr1.4:63553415-63553434 MS.gene004953:intron 15.0%
!!! AAATTGCATTTTTATTTTAC+CGG + chr1.4:63553338-63553357 None:intergenic 15.0%
!!! AATATTATGTTTTGTAGTTT+TGG - chr1.4:63553044-63553063 MS.gene004953:intron 15.0%
!!! ATATGTATTTTTTATTTGAG+TGG - chr1.4:63553703-63553722 MS.gene004953:intron 15.0%
!!! TATGTATTTTTTATTTGAGT+GGG - chr1.4:63553704-63553723 MS.gene004953:intron 15.0%
!! ACTATATAGTTAATTTAGAC+TGG - chr1.4:63553761-63553780 MS.gene004953:intron 20.0%
!! ATTAAAATAGAAGTGAGTAT+TGG + chr1.4:63552125-63552144 None:intergenic 20.0%
!! CCAAATAGATTAATGATATT+TGG + chr1.4:63553827-63553846 None:intergenic 20.0%
!! CCAAATATCATTAATCTATT+TGG - chr1.4:63553824-63553843 MS.gene004953:intron 20.0%
!! TAACAAAGCATATAAGATTA+CGG + chr1.4:63553950-63553969 None:intergenic 20.0%
!! TAATCACAAGTATATATAGA+AGG + chr1.4:63551764-63551783 None:intergenic 20.0%
!! TGTTAAATGTTGCAAATATA+TGG + chr1.4:63553531-63553550 None:intergenic 20.0%
!!! ATATAAGTTCTATTGATTAG+AGG - chr1.4:63552447-63552466 MS.gene004953:intron 20.0%
!!! ATATAAGTTCTATTGATTTG+AGG - chr1.4:63552671-63552690 MS.gene004953:intron 20.0%
!!! ATGTATTTTTTATTTGAGTG+GGG - chr1.4:63553705-63553724 MS.gene004953:intron 20.0%
!!! ATTTACTGAACCATTTTTTT+TGG - chr1.4:63552726-63552745 MS.gene004953:intron 20.0%
!!! CTTTCATTTTACATTTATCT+GGG + chr1.4:63552771-63552790 None:intergenic 20.0%
!!! TAATGACTATTTTTGTATTG+TGG - chr1.4:63553989-63554008 MS.gene004953:CDS 20.0%
!!! TATAAGTTCTATTGATTAGA+GGG - chr1.4:63552448-63552467 MS.gene004953:intron 20.0%
!!! TATAAGTTCTATTGATTTGA+GGG - chr1.4:63552672-63552691 MS.gene004953:intron 20.0%
!!! TCTTTCATTTTACATTTATC+TGG + chr1.4:63552772-63552791 None:intergenic 20.0%
!!! TTAAACTTTTTAATCCTAAG+AGG - chr1.4:63553360-63553379 MS.gene004953:intron 20.0%
!!! TTTCATTTTACATTTATCTG+GGG + chr1.4:63552770-63552789 None:intergenic 20.0%
! AAATATGTTAGATGAGGAAA+AGG + chr1.4:63552087-63552106 None:intergenic 25.0%
! AGTTGAGAAAAATTCAACTA+CGG + chr1.4:63551652-63551671 None:intergenic 25.0%
! ATACATCATCAAATTACATG+AGG + chr1.4:63553094-63553113 None:intergenic 25.0%
! TGTAAAATTGACAATTCACT+AGG + chr1.4:63552248-63552267 None:intergenic 25.0%
! TGTACGAATATAAGTTCTAT+TGG - chr1.4:63552440-63552459 MS.gene004953:intron 25.0%
! TGTAGAAAATATGTTAGATG+AGG + chr1.4:63552093-63552112 None:intergenic 25.0%
!! TCTAGAATCTAAAATTTAGG+AGG - chr1.4:63553418-63553437 MS.gene004953:intron 25.0%
!!! ATACTTGTGATTAATTTGTG+TGG - chr1.4:63551771-63551790 MS.gene004953:CDS 25.0%
AAATAGTCATTACGCATGAT+TGG + chr1.4:63553981-63554000 None:intergenic 30.0%
AAATTACATGAGGCCAATTA+TGG + chr1.4:63553084-63553103 None:intergenic 30.0%
AATTACATGAGGCCAATTAT+GGG + chr1.4:63553083-63553102 None:intergenic 30.0%
ACTTCGAGATGGATAAAAAT+AGG - chr1.4:63553919-63553938 MS.gene004953:intron 30.0%
ATTGAGCTCAAAACTCAAAA+GGG + chr1.4:63552168-63552187 None:intergenic 30.0%
CTTCGAGATGGATAAAAATA+GGG - chr1.4:63553920-63553939 MS.gene004953:intron 30.0%
GCATATGCATAAAATATGCA+AGG + chr1.4:63552954-63552973 None:intergenic 30.0%
TTACAATACCTAGTTAATCC+CGG - chr1.4:63553264-63553283 MS.gene004953:intron 30.0%
TTCGAGATGGATAAAAATAG+GGG - chr1.4:63553921-63553940 MS.gene004953:intron 30.0%
TTGAAAATTGTTGCATGTGT+AGG - chr1.4:63553784-63553803 MS.gene004953:intron 30.0%
! TATTGATTAGAGGGTTTCAA+AGG - chr1.4:63552457-63552476 MS.gene004953:intron 30.0%
!! TATTGATTTGAGGGTTTCAA+AGG - chr1.4:63552681-63552700 MS.gene004953:intron 30.0%
!! TTGTGATTAATTTGTGTGGT+TGG - chr1.4:63551775-63551794 MS.gene004953:CDS 30.0%
!!! TACTGAACCATTTTTTTTGG+TGG - chr1.4:63552729-63552748 MS.gene004953:intron 30.0%
AACTACAAGTGCAATTGCTA+AGG + chr1.4:63554170-63554189 None:intergenic 35.0%
AAGAGACTAAAACTTCGAGA+TGG - chr1.4:63553908-63553927 MS.gene004953:intron 35.0%
AAGATATGCATTTGGAAGGA+TGG - chr1.4:63551593-63551612 MS.gene004953:CDS 35.0%
ACTACAAGTGCAATTGCTAA+GGG + chr1.4:63554169-63554188 None:intergenic 35.0%
AGTGACAAGCCTCAATAAAA+GGG + chr1.4:63552034-63552053 None:intergenic 35.0%
ATACATTAAGCCAAACGTCA+TGG + chr1.4:63554092-63554111 None:intergenic 35.0%
ATCCTAAGAGGCAATTTAAG+AGG - chr1.4:63553372-63553391 MS.gene004953:intron 35.0%
CATAAAATATGCAAGGTCTG+GGG + chr1.4:63552947-63552966 None:intergenic 35.0%
CTCATAGTAAAATAGGTCTC+CGG - chr1.4:63553316-63553335 MS.gene004953:intron 35.0%
GAGGCTTGTCACTAATAATA+TGG - chr1.4:63552041-63552060 MS.gene004953:intron 35.0%
GATTGAGCTCAAAACTCAAA+AGG + chr1.4:63552169-63552188 None:intergenic 35.0%
GCATAAAATATGCAAGGTCT+GGG + chr1.4:63552948-63552967 None:intergenic 35.0%
GCATTATCATTGGATCTTCT+GGG - chr1.4:63554279-63554298 MS.gene004953:intron 35.0%
TAATCCTCATCATCAACCTT+TGG - chr1.4:63554378-63554397 MS.gene004953:CDS 35.0%
TAATGCAGGGAAGATATACA+AGG - chr1.4:63551804-63551823 MS.gene004953:CDS 35.0%
TACAATACCTAGTTAATCCC+GGG - chr1.4:63553265-63553284 MS.gene004953:intron 35.0%
TAGTGACAAGCCTCAATAAA+AGG + chr1.4:63552035-63552054 None:intergenic 35.0%
TATGTTAGATGAGGAAAAGG+TGG + chr1.4:63552084-63552103 None:intergenic 35.0%
TGCATAAAATATGCAAGGTC+TGG + chr1.4:63552949-63552968 None:intergenic 35.0%
TTAGCAATTGCACTTGTAGT+TGG - chr1.4:63554169-63554188 MS.gene004953:intron 35.0%
TTCGGCTCTCATAGTAAAAT+AGG - chr1.4:63553309-63553328 MS.gene004953:intron 35.0%
! AAATTTTGTCCTAGCTCAGT+TGG + chr1.4:63553003-63553022 None:intergenic 35.0%
! AGGCAATTTAAGAGGCATAA+AGG - chr1.4:63553380-63553399 MS.gene004953:intron 35.0%
! CTCTTATTTCCACCCATAAT+TGG - chr1.4:63553068-63553087 MS.gene004953:intron 35.0%
! TCAACTTCTTGGTTCTTTAG+TGG - chr1.4:63552005-63552024 MS.gene004953:intron 35.0%
! TGATGATGAGGATTAGTATG+AGG + chr1.4:63554373-63554392 None:intergenic 35.0%
! TGGTTGGTTGAATTAATGCA+GGG - chr1.4:63551791-63551810 MS.gene004953:CDS 35.0%
! TTGGATGATTTTACACCATC+TGG - chr1.4:63553843-63553862 MS.gene004953:intron 35.0%
!!! TGAACCATTTTTTTTGGTGG+TGG - chr1.4:63552732-63552751 MS.gene004953:intron 35.0%
ACTACTACGAACTTAGAGCA+AGG + chr1.4:63551707-63551726 None:intergenic 40.0%
ACTTGCATCTTTGTCTTTGC+TGG - chr1.4:63551669-63551688 MS.gene004953:CDS 40.0%
ATCTTTGTCTTTGCTGGAGA+AGG - chr1.4:63551675-63551694 MS.gene004953:CDS 40.0%
CCAGAAGATATGCATTTGGA+AGG - chr1.4:63551589-63551608 MS.gene004953:CDS 40.0%
CCTTAGAGCTGTCTACTATT+GGG - chr1.4:63551978-63551997 MS.gene004953:intron 40.0%
CCTTCCAAATGCATATCTTC+TGG + chr1.4:63551592-63551611 None:intergenic 40.0%
CTACTACGAACTTAGAGCAA+GGG + chr1.4:63551706-63551725 None:intergenic 40.0%
GAGATGGATAAAAATAGGGG+AGG - chr1.4:63553924-63553943 MS.gene004953:intron 40.0%
GCAACCAGAAGATATGCATT+TGG - chr1.4:63551585-63551604 MS.gene004953:CDS 40.0%
GGCATTATCATTGGATCTTC+TGG - chr1.4:63554278-63554297 MS.gene004953:intron 40.0%
GTTGGAGATGGCATTATCAT+TGG - chr1.4:63554269-63554288 MS.gene004953:intron 40.0%
TAGTAATCTTCAGGAGCCAA+AGG + chr1.4:63554397-63554416 None:intergenic 40.0%
TATCATTGGATCTTCTGGGT+GGG - chr1.4:63554283-63554302 MS.gene004953:intron 40.0%
TCATCTAGCATGCATGTATC+TGG - chr1.4:63551898-63551917 MS.gene004953:CDS 40.0%
TCCTTAGAGCTGTCTACTAT+TGG - chr1.4:63551977-63551996 MS.gene004953:intron 40.0%
TGCCTCTTAAATTGCCTCTT+AGG + chr1.4:63553377-63553396 None:intergenic 40.0%
TTATCATTGGATCTTCTGGG+TGG - chr1.4:63554282-63554301 MS.gene004953:intron 40.0%
TTCAACTACGGTTGCTCTTA+TGG + chr1.4:63551640-63551659 None:intergenic 40.0%
TTCATGCATGCTCCATCAAA+CGG + chr1.4:63554217-63554236 None:intergenic 40.0%
TTCCTATGAAAGAGGAGGTT+CGG - chr1.4:63553291-63553310 MS.gene004953:intron 40.0%
! GTGGTTGGTTGAATTAATGC+AGG - chr1.4:63551790-63551809 MS.gene004953:CDS 40.0%
! TATTGGGTTGCTCAACTTCT+TGG - chr1.4:63551994-63552013 MS.gene004953:intron 40.0%
! TGATGAGGATTAGTATGAGG+AGG + chr1.4:63554370-63554389 None:intergenic 40.0%
!! TTCAATGTGGCATTTGGTGT+TGG - chr1.4:63554037-63554056 MS.gene004953:CDS 40.0%
ACGGTTGCTCTTATGGAATC+TGG + chr1.4:63551633-63551652 None:intergenic 45.0%
ACTGCCATTGATCCCAAAAG+AGG - chr1.4:63554124-63554143 MS.gene004953:CDS 45.0%
ATCAACCCTGCTGTTACCTT+TGG - chr1.4:63551928-63551947 MS.gene004953:CDS 45.0%
CCCAATAGTAGACAGCTCTA+AGG + chr1.4:63551981-63552000 None:intergenic 45.0%
CCTCTTTCATAGGAAGAACC+CGG + chr1.4:63553286-63553305 None:intergenic 45.0%
CTCTTTCATAGGAAGAACCC+GGG + chr1.4:63553285-63553304 None:intergenic 45.0%
CTTGAGATTGCCATGACGTT+TGG - chr1.4:63554079-63554098 MS.gene004953:CDS 45.0%
GCTATTGCAGCTCCAAGAAA+TGG + chr1.4:63554310-63554329 None:intergenic 45.0%
GGGAAGATATACAAGGATGC+AGG - chr1.4:63551811-63551830 MS.gene004953:CDS 45.0%
GGTTCTTCCTATGAAAGAGG+AGG - chr1.4:63553286-63553305 MS.gene004953:intron 45.0%
TACATGAGGCCAATTATGGG+TGG + chr1.4:63553080-63553099 None:intergenic 45.0%
TCTAGCATGCATGTATCTGG+TGG - chr1.4:63551901-63551920 MS.gene004953:CDS 45.0%
TGCATTTGGAAGGATGGATG+AGG - chr1.4:63551599-63551618 MS.gene004953:CDS 45.0%
TTGCCTGTTATAGAGACCAC+AGG - chr1.4:63554012-63554031 MS.gene004953:CDS 45.0%
TTTCATGCGATACGACCAGA+TGG + chr1.4:63553861-63553880 None:intergenic 45.0%
! GCTTTTGGACCTGCTTTAGT+GGG - chr1.4:63554247-63554266 MS.gene004953:intron 45.0%
! TAAGGGTGCAATTGTGCCAA+TGG + chr1.4:63554152-63554171 None:intergenic 45.0%
! TAGTGGCTGCCCTTTTATTG+AGG - chr1.4:63552022-63552041 MS.gene004953:intron 45.0%
! TTGTCCTAGCTCAGTTGGTA+TGG + chr1.4:63552998-63553017 None:intergenic 45.0%
!! GTTACCTTTGGTGCTCTTCT+TGG - chr1.4:63551940-63551959 MS.gene004953:CDS 45.0%
!!! ATTTTTTTTGGTGGTGGCCG+GGG - chr1.4:63552738-63552757 MS.gene004953:intron 45.0%
!!! CATTTTTTTTGGTGGTGGCC+GGG - chr1.4:63552737-63552756 MS.gene004953:intron 45.0%
!!! CCATTTTTTTTGGTGGTGGC+CGG - chr1.4:63552736-63552755 MS.gene004953:intron 45.0%
!! TAATAATATATATCAATAAA+TGG + chr1.4:63552515-63552534 None:intergenic 5.0%
!!! ATTTTATTTTGAAATAAAAT+TGG - chr1.4:63552198-63552217 MS.gene004953:intron 5.0%
!!! TTTTATTTTGAAATAAAATT+GGG - chr1.4:63552199-63552218 MS.gene004953:intron 5.0%
AAGGATGCAGGTGCATCATC+TGG - chr1.4:63551823-63551842 MS.gene004953:CDS 50.0%
AATGCCACATTGAACGCCTG+TGG + chr1.4:63554031-63554050 None:intergenic 50.0%
ACGCCTGTGGTCTCTATAAC+AGG + chr1.4:63554018-63554037 None:intergenic 50.0%
AGCATGCATGAACCCTGCTA+GGG - chr1.4:63554225-63554244 MS.gene004953:intron 50.0%
AGCCGAACCTCCTCTTTCAT+AGG + chr1.4:63553296-63553315 None:intergenic 50.0%
CAGGCGTTCAATGTGGCATT+TGG - chr1.4:63554031-63554050 MS.gene004953:CDS 50.0%
CATCTCCAACCCACTAAAGC+AGG + chr1.4:63554259-63554278 None:intergenic 50.0%
CCGGCCACCACCAAAAAAAA+TGG + chr1.4:63552739-63552758 None:intergenic 50.0%
CCGGGTTCTTCCTATGAAAG+AGG - chr1.4:63553283-63553302 MS.gene004953:intron 50.0%
GAGCACCAAAGGTAACAGCA+GGG + chr1.4:63551936-63551955 None:intergenic 50.0%
GAGTGGGGTGTATTCACATC+TGG - chr1.4:63553720-63553739 MS.gene004953:intron 50.0%
GGAAGAACCCGGGATTAACT+AGG + chr1.4:63553275-63553294 None:intergenic 50.0%
GTTGGAGCAAACATCCTAGC+TGG - chr1.4:63554187-63554206 MS.gene004953:intron 50.0%
TCGTCCATACCAACTGAGCT+AGG - chr1.4:63552991-63553010 MS.gene004953:intron 50.0%
TGCATCATCTGGTGAGCTAG+TGG - chr1.4:63551834-63551853 MS.gene004953:CDS 50.0%
TTCTGGGTGGGTCCATTTCT+TGG - chr1.4:63554295-63554314 MS.gene004953:CDS 50.0%
! AGAGCACCAAAGGTAACAGC+AGG + chr1.4:63551937-63551956 None:intergenic 50.0%
! ATGAACCCTGCTAGGGCTTT+TGG - chr1.4:63554232-63554251 MS.gene004953:intron 50.0%
! GGCTTTTGGACCTGCTTTAG+TGG - chr1.4:63554246-63554265 MS.gene004953:intron 50.0%
! GTGCCAATGGTGCCTCTTTT+GGG + chr1.4:63554139-63554158 None:intergenic 50.0%
! TGTGCCAATGGTGCCTCTTT+TGG + chr1.4:63554140-63554159 None:intergenic 50.0%
! TTGGACCTGCTTTAGTGGGT+TGG - chr1.4:63554251-63554270 MS.gene004953:intron 50.0%
! TTTATCTGGGGTTCGAACCC+CGG + chr1.4:63552758-63552777 None:intergenic 50.0%
!! ACCACCAAGAAGAGCACCAA+AGG + chr1.4:63551947-63551966 None:intergenic 50.0%
!! ACCTTTGGTGCTCTTCTTGG+TGG - chr1.4:63551943-63551962 MS.gene004953:CDS 50.0%
!! CTGCTTTAGTGGGTTGGAGA+TGG - chr1.4:63554257-63554276 MS.gene004953:intron 50.0%
!! GATCCCAAAAGAGGCACCAT+TGG - chr1.4:63554133-63554152 MS.gene004953:CDS 50.0%
!! GGAGCCAAAGGTTGATGATG+AGG + chr1.4:63554385-63554404 None:intergenic 50.0%
!! TGGCATTTGGTGTTGGTGCT+GGG - chr1.4:63554044-63554063 MS.gene004953:CDS 50.0%
!!! GGTGCCTCTTTTGGGATCAA+TGG + chr1.4:63554131-63554150 None:intergenic 50.0%
CAGGTCCAAAAGCCCTAGCA+GGG + chr1.4:63554240-63554259 None:intergenic 55.0%
CATCAAACGGTCCACCAGCT+AGG + chr1.4:63554204-63554223 None:intergenic 55.0%
GAGACCACAGGCGTTCAATG+TGG - chr1.4:63554024-63554043 MS.gene004953:CDS 55.0%
GAGCATGCATGAACCCTGCT+AGG - chr1.4:63554224-63554243 MS.gene004953:intron 55.0%
GGAGCAAACATCCTAGCTGG+TGG - chr1.4:63554190-63554209 MS.gene004953:intron 55.0%
! CTAGCTGGTGGACCGTTTGA+TGG - chr1.4:63554202-63554221 MS.gene004953:intron 55.0%
!! GTGGCATTTGGTGTTGGTGC+TGG - chr1.4:63554043-63554062 MS.gene004953:CDS 55.0%
GCAGGTCCAAAAGCCCTAGC+AGG + chr1.4:63554241-63554260 None:intergenic 60.0%
! AAGGTCTGGGGTTCGAACCC+CGG + chr1.4:63552935-63552954 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 63551582 63554417 63551582 ID=MS.gene004953
chr1.4 mRNA 63551582 63554417 63551582 ID=MS.gene004953.t1;Parent=MS.gene004953
chr1.4 exon 63554285 63554417 63554285 ID=MS.gene004953.t1.exon1;Parent=MS.gene004953.t1
chr1.4 CDS 63554285 63554417 63554285 ID=cds.MS.gene004953.t1;Parent=MS.gene004953.t1
chr1.4 exon 63553937 63554187 63553937 ID=MS.gene004953.t1.exon2;Parent=MS.gene004953.t1
chr1.4 CDS 63553937 63554187 63553937 ID=cds.MS.gene004953.t1;Parent=MS.gene004953.t1
chr1.4 exon 63551582 63551974 63551582 ID=MS.gene004953.t1.exon3;Parent=MS.gene004953.t1
chr1.4 CDS 63551582 63551974 63551582 ID=cds.MS.gene004953.t1;Parent=MS.gene004953.t1
Gene Sequence

>MS.gene004953

ATGGCAACCAGAAGATATGCATTTGGAAGGATGGATGAGGCAAATCATCCAGATTCCATAAGAGCAACCGTAGTTGAATTTTTCTCAACTTGCATCTTTGTCTTTGCTGGAGAAGGATCTGCCCTTGCTCTAAGTTCGTAGTAGTGTAGTACCATATTATATATCTATATGGATACACTCCTTCTATATATACTTGTGATTAATTTGTGTGGTTGGTTGAATTAATGCAGGGAAGATATACAAGGATGCAGGTGCATCATCTGGTGAGCTAGTGGTACTAGCACTAGCTCATGCATTTTCACTATTTGCAGCTATATCATCTAGCATGCATGTATCTGGTGGACATATCAACCCTGCTGTTACCTTTGGTGCTCTTCTTGGTGGTAGAATCTCTGTCCTTAGAGCTGTCTACTATTGGGTTGCTCAACTTCTTGGTTCTTTAGTGGCTGCCCTTTTATTGAGGCTTGTCACTAATAATATGGTATAACATTACTCAATTCCACCTTTTCCTCATCTAACATATTTTCTACATTATTTTATCCAATACTCACTTCTATTTTAATCTTAATCAGATAGACTTTAACCCTTTTGAGTTTTGAGCTCAATCACTTGTTTTATTTTATTTTGAAATAAAATTGGGAAGAGAAAATGACTCATCACTTTCCTAGTGAATTGTCAATTTTACACTATTTTGCATAGATTAAGAAAAACAAATAGAAGAGATAAAACAATAAGTTTACTAAATTACTCTTATTGATATATATTATTATACGACAAATGTAAAATGAAAGATATTCACTTGAGATAGAGCTTATAAATGTAAAATGAAAGATATTCATTTGAGATAGAGCTTATACTTGTACGAATATAAGTTCTATTGATTAGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAAATTTACTGAACCATTTATTGATATATATTATTATACGACAAATGTAAAATGAAAGATATTCACTTGAGATAGAGCTTATAAATGTAAAATGAAAGATATTCACTTGAGATAATGAAAGATATTCACTTGAGATAGAGCTTATACTTGTACGAATATAAGTTCTATTGGAATATAAGTTCTATTGATTTGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAAATTTACTGAACCATTTTTTTTGGTGGTGGCCGGGGTTCGAACCCCAGATAAATGTAAAATGAAAGATATTCACTTGAGATAATGAAAGATATTCACTTGAGATAGAGCTTATACTTGTACGAATATAAGTTCTATTGATTAGAGGGTTTCAAAGGACTTGAATTTATAAAGTCAAAAATTTACTGAACCATTTTTTTTGGTGGTGGCCGGGGTTCGAACCCCAGACCTTGCATATTTTATGCATATGCTTTGCATATTTTATGCATCGTCCATACCAACTGAGCTAGGACAAAATTTACTGAACAATTATCACTTATTAATATTATGTTTTGTAGTTTTGGTCTCTTATTTCCACCCATAATTGGCCTCATGTAATTTGATGATGTATAGTGATCAACACTTGTTTTCTTAGTTTTTTACTTAACACGATCAACACTTACAATTTCAACTTTAAAAATTAATTATTTTATAATTTGATTATCGATATACAAATAACTAATCTACAAAATGTTTTATGAAAACTCAATCTTCTCTACACTTACAATACCTAGTTAATCCCGGGTTCTTCCTATGAAAGAGGAGGTTCGGCTCTCATAGTAAAATAGGTCTCCGGTAAAATAAAAATGCAATTTTTTTAAACTTTTTAATCCTAAGAGGCAATTTAAGAGGCATAAAGGAAGACTACAAAAAAATCTAGAATCTAAAATTTAGGAGGTATATATTAGCAATATTCTCAACATTCAACGTCAATCATATAATGAAATTAGCAATTATATTTTTCAAACTTCAACATATATTAGAACCATATATTTGCAACATTTAACAAATTAGCAATTATGCACTGAAATTTTAAAAAGTAATTACTAATAAACATTAAGCAACGTATAACAAAATTCGTGTAGCTAATCTTCATTAAAAAATCATAATTAGAAGAACTTCTAAATATGAAATGTATTGAATAAAGAAAAAGAATGAGCATATGTATTTTTTATTTGAGTGGGGTGTATTCACATCTGGTCAATTCACATGCACTTAACTATATAGTTAATTTAGACTGGTTGAAAATTGTTGCATGTGTAGGAAAAAAAAAAAGAGTGCCCAAATATCATTAATCTATTTGGATGATTTTACACCATCTGGTCGTATCGCATGAAACGTCATATTTTAGTTGACGAATTGAATAAGAGACTAAAACTTCGAGATGGATAAAAATAGGGGAGGCCGTAATCTTATATGCTTTGTTATTCTCATGCCAATCATGCGTAATGACTATTTTTGTATTGTGGTTGCCTGTTATAGAGACCACAGGCGTTCAATGTGGCATTTGGTGTTGGTGCTGGGAACGCTCTTGTACTTGAGATTGCCATGACGTTTGGCTTAATGTATGTTGTGTATGCAACTGCCATTGATCCCAAAAGAGGCACCATTGGCACAATTGCACCCTTAGCAATTGCACTTGTAGTTGGAGCAAACATCCTAGCTGGTGGACCGTTTGATGGAGCATGCATGAACCCTGCTAGGGCTTTTGGACCTGCTTTAGTGGGTTGGAGATGGCATTATCATTGGATCTTCTGGGTGGGTCCATTTCTTGGAGCTGCAATAGCAGCACTTTTATATGAATATATTATGGTTTCAGTTGAGCCTCCTCATACTAATCCTCATCATCAACCTTTGGCTCCTGAAGATTACTAG
Protein sequence

>MS.gene004953.t1

MATRRYAFGRMDEANHPDSIRATVVEFFSTCIFVFAGEGSALALRKIYKDAGASSGELVVLALAHAFSLFAAISSSMHVSGGHINPAVTFGALLGGRISVLRAVYYWVAQLLGSLVAALLLRLVTNNMRPQAFNVAFGVGAGNALVLEIAMTFGLMYVVYATAIDPKRGTIGTIAPLAIALVVGANILAGGPFDGACMNPARAFGPALVGWRWHYHWIFWVGPFLGAAIAALLYEYIMVSVEPPHTNPHHQPLAPEDY