AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene042849

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042849.t1 MTR_6g069040 98.039 255 5 0 1 255 1 255 0.0 497
MS.gene042849.t1 MTR_1g077540 79.070 258 51 1 1 255 1 258 5.62e-151 421
MS.gene042849.t1 MTR_1g115405 57.255 255 106 1 1 255 1 252 2.48e-100 293
MS.gene042849.t1 MTR_7g023350 56.863 255 107 1 1 255 1 252 2.94e-94 277
MS.gene042849.t1 MTR_4g063090 54.902 255 112 1 1 255 1 252 6.04e-94 276
MS.gene042849.t1 MTR_7g103030 57.647 255 103 2 1 255 1 250 1.78e-92 273
MS.gene042849.t1 MTR_2g101370 54.800 250 109 1 4 253 3 248 1.09e-91 271
MS.gene042849.t1 MTR_8g013680 53.846 247 110 1 7 253 5 247 1.27e-85 255
MS.gene042849.t1 MTR_5g012810 53.333 240 111 1 5 244 3 241 2.31e-73 224
MS.gene042849.t1 MTR_1g006490 44.400 250 131 3 5 254 3 244 1.43e-70 217
MS.gene042849.t1 MTR_1g006590 45.200 250 129 3 5 254 3 244 2.87e-67 209
MS.gene042849.t1 MTR_2g060360 40.343 233 138 1 10 242 8 239 1.15e-54 177
MS.gene042849.t1 MTR_2g094270 37.037 243 136 6 12 238 31 272 1.83e-35 128
MS.gene042849.t1 MTR_5g010150 39.419 241 128 8 21 248 52 287 1.97e-35 128
MS.gene042849.t1 MTR_5g082070 38.723 235 128 6 21 243 55 285 2.46e-35 127
MS.gene042849.t1 MTR_7g101190 36.910 233 131 5 21 238 39 270 6.24e-35 127
MS.gene042849.t1 MTR_4g415300 38.034 234 130 6 21 243 56 285 1.67e-34 125
MS.gene042849.t1 MTR_3g118010 37.118 229 130 5 21 238 37 262 1.72e-34 125
MS.gene042849.t1 MTR_8g098375 38.298 235 129 7 21 243 52 282 2.34e-34 125
MS.gene042849.t1 MTR_3g070210 37.872 235 130 6 21 243 55 285 6.75e-34 124
MS.gene042849.t1 MTR_5g063930 34.836 244 142 7 15 255 31 260 1.59e-33 122
MS.gene042849.t1 MTR_4g059390 37.712 236 125 7 21 238 39 270 2.15e-33 122
MS.gene042849.t1 MTR_1g095070 39.779 181 97 4 69 238 25 204 8.42e-31 114
MS.gene042849.t1 MTR_5g079570 33.051 236 146 4 20 255 39 262 1.16e-28 110
MS.gene042849.t1 MTR_2g017620 32.895 228 144 5 16 242 19 238 9.75e-28 107
MS.gene042849.t1 MTR_3g008920 33.475 236 144 6 20 251 25 251 1.15e-27 107
MS.gene042849.t1 MTR_2g017620 32.895 228 144 5 16 242 33 252 1.64e-27 107
MS.gene042849.t1 MTR_2g017590 30.435 253 152 6 1 243 13 251 2.74e-27 106
MS.gene042849.t1 MTR_2g104940 32.735 223 142 3 25 247 84 298 1.77e-26 105
MS.gene042849.t1 MTR_2g017660 29.658 263 155 7 1 253 16 258 7.10e-26 102
MS.gene042849.t1 MTR_4g006730 32.110 218 140 3 25 242 79 288 5.16e-25 100
MS.gene042849.t1 MTR_8g087710 31.718 227 142 5 20 243 38 254 8.57e-25 99.8
MS.gene042849.t1 MTR_2g017620 35.542 166 103 3 78 242 9 171 5.72e-24 95.5
MS.gene042849.t1 MTR_4g084880 29.204 226 151 3 20 243 34 252 8.51e-24 97.1
MS.gene042849.t1 MTR_2g094270 35.545 211 122 5 12 209 31 240 9.26e-24 97.1
MS.gene042849.t1 MTR_2g017610 29.583 240 153 6 10 243 24 253 2.29e-23 95.9
MS.gene042849.t1 MTR_7g010650 32.051 234 147 7 21 254 37 258 7.64e-23 94.7
MS.gene042849.t1 MTR_2g017570 29.515 227 147 5 20 243 37 253 1.82e-22 93.6
MS.gene042849.t1 MTR_8g087720 29.461 241 147 6 12 243 27 253 3.71e-22 92.8
MS.gene042849.t1 MTR_4g006630 31.100 209 136 3 34 242 1 201 5.91e-22 90.9
MS.gene042849.t1 MTR_1g070385 27.897 233 158 5 21 253 62 284 6.28e-22 95.1
MS.gene042849.t1 MTR_4g006730 31.100 209 136 3 34 242 1 201 7.35e-22 90.9
MS.gene042849.t1 MTR_3g070210 33.175 211 126 5 21 219 55 262 8.05e-22 92.0
MS.gene042849.t1 MTR_4g415300 33.898 177 108 4 21 193 56 227 2.26e-19 84.3
MS.gene042849.t1 MTR_1g097840 29.018 224 148 4 25 245 78 293 2.98e-19 85.1
MS.gene042849.t1 MTR_5g010150 36.364 165 95 5 21 179 52 212 3.33e-19 83.6
MS.gene042849.t1 MTR_1g095070 36.478 159 89 4 69 216 25 182 7.95e-19 82.0
MS.gene042849.t1 MTR_8g098375 34.545 165 98 5 21 179 52 212 3.57e-18 81.3
MS.gene042849.t1 MTR_3g070210 33.742 163 100 3 21 178 55 214 6.52e-18 80.1
MS.gene042849.t1 MTR_4g059390 35.294 170 95 5 21 179 39 204 3.09e-17 78.2
MS.gene042849.t1 MTR_4g075590 26.772 254 155 7 11 242 27 271 3.68e-15 73.9
MS.gene042849.t1 MTR_4g075570 28.512 242 144 8 13 234 43 275 5.67e-15 73.6
MS.gene042849.t1 MTR_4g006730 32.335 167 105 3 25 191 79 237 3.45e-14 70.9
MS.gene042849.t1 MTR_8g468730 36.082 97 43 2 146 242 8 85 3.36e-13 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene042849.t1 AT1G73190 74.242 264 58 3 2 255 5 268 7.29e-126 358
MS.gene042849.t1 AT1G17810 73.764 263 60 3 2 255 5 267 4.31e-124 353
MS.gene042849.t1 AT4G01470 58.431 255 103 1 1 255 1 252 7.29e-102 296
MS.gene042849.t1 AT2G36830 55.686 255 109 1 1 255 1 251 2.32e-98 288
MS.gene042849.t1 AT3G26520 56.250 256 108 2 1 255 1 253 2.62e-97 285
MS.gene042849.t1 AT3G16240 52.209 249 116 1 7 255 5 250 4.72e-84 251
MS.gene042849.t1 AT5G47450 51.304 230 112 0 5 234 3 232 9.24e-78 235
MS.gene042849.t1 AT4G17340 51.250 240 117 0 5 244 3 242 6.18e-72 221
MS.gene042849.t1 AT2G25810 45.020 251 130 3 4 254 2 244 3.60e-67 208
MS.gene042849.t1 AT3G47440 41.593 226 129 3 18 242 20 243 6.77e-51 167
MS.gene042849.t1 AT2G37170 38.153 249 132 8 8 238 24 268 1.05e-37 134
MS.gene042849.t1 AT2G37170 38.153 249 132 8 8 238 67 311 1.49e-37 134
MS.gene042849.t1 AT2G37180 36.538 260 137 8 3 238 13 268 6.20e-37 132
MS.gene042849.t1 AT3G54820 37.607 234 128 7 21 238 38 269 1.95e-35 128
MS.gene042849.t1 AT2G39010 36.864 236 127 6 21 238 38 269 3.47e-35 127
MS.gene042849.t1 AT3G53420 38.559 236 123 7 21 238 39 270 5.12e-35 127
MS.gene042849.t1 AT3G53420 38.559 236 123 7 21 238 39 270 5.12e-35 127
MS.gene042849.t1 AT1G01620 40.598 234 124 7 21 243 52 281 4.02e-34 124
MS.gene042849.t1 AT2G16850 38.961 231 123 7 21 238 36 261 6.62e-34 124
MS.gene042849.t1 AT4G00430 39.301 229 125 6 21 238 53 278 8.62e-34 124
MS.gene042849.t1 AT2G45960 39.744 234 126 7 21 243 52 281 1.79e-33 123
MS.gene042849.t1 AT4G35100 37.555 229 129 5 21 238 38 263 4.89e-33 121
MS.gene042849.t1 AT4G35100 37.555 229 129 5 21 238 38 263 4.89e-33 121
MS.gene042849.t1 AT3G61430 39.316 234 127 7 21 243 52 281 7.11e-33 121
MS.gene042849.t1 AT3G61430 39.316 234 127 7 21 243 52 281 7.11e-33 121
MS.gene042849.t1 AT4G23400 38.655 238 123 8 21 243 53 282 7.99e-33 121
MS.gene042849.t1 AT5G60660 36.864 236 127 7 21 238 39 270 3.97e-32 119
MS.gene042849.t1 AT1G01620 42.784 194 98 5 61 243 18 209 2.33e-31 115
MS.gene042849.t1 AT5G37820 29.596 223 150 3 21 243 43 258 3.78e-27 106
MS.gene042849.t1 AT5G37820 29.596 223 150 3 21 243 79 294 6.51e-27 106
MS.gene042849.t1 AT1G80760 31.651 218 141 4 25 242 84 293 2.55e-25 102
MS.gene042849.t1 AT5G37810 29.148 223 151 3 21 243 43 258 3.35e-24 98.6
MS.gene042849.t1 AT4G19030 32.035 231 143 6 20 243 54 277 6.30e-24 97.8
MS.gene042849.t1 AT4G18910 31.034 232 144 6 20 243 51 274 1.39e-23 97.1
MS.gene042849.t1 AT1G31885 33.043 230 124 6 20 234 42 256 4.64e-23 96.3
MS.gene042849.t1 AT1G52180 62.857 70 26 0 139 208 47 116 4.14e-22 89.0
MS.gene042849.t1 AT2G45960 36.548 197 114 5 21 209 52 245 2.92e-21 90.9
MS.gene042849.t1 AT2G45960 35.749 207 119 6 21 216 52 255 3.06e-21 90.5
MS.gene042849.t1 AT4G10380 29.911 224 146 4 25 245 82 297 1.08e-20 89.4
MS.gene042849.t1 AT1G31885 36.571 175 86 4 78 237 41 205 1.80e-20 88.2
MS.gene042849.t1 AT3G06100 26.749 243 167 4 3 243 28 261 2.17e-20 88.2
MS.gene042849.t1 AT2G39010 34.911 169 95 4 21 178 38 202 9.87e-20 85.1
MS.gene042849.t1 AT3G06100 27.477 222 150 4 24 243 1 213 1.35e-18 82.4
MS.gene042849.t1 AT4G00430 37.126 167 98 4 21 183 53 216 6.24e-18 80.1
MS.gene042849.t1 AT2G34390 27.556 225 148 6 20 237 47 263 9.69e-13 67.0
MS.gene042849.t1 AT2G34390 28.509 228 134 7 24 237 9 221 1.48e-12 65.9
MS.gene042849.t1 AT4G19030 31.638 177 107 6 20 189 54 223 1.95e-12 65.1
MS.gene042849.t1 AT3G06100 28.261 138 97 2 106 243 45 180 2.58e-11 61.6

Find 80 sgRNAs with CRISPR-Local

Find 124 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TATTGGATTGCTCAACTTCT+AGG 0.222866 6.3:+66314597 MS.gene042849:CDS
ACTTTCATCTTTGTCTTTGC+TGG 0.227521 6.3:+66314040 MS.gene042849:CDS
GGCACCACCACTGGATCTTT+TGG 0.236177 6.3:+66315426 MS.gene042849:CDS
TCCTTCGTGCTGTCTACTAT+TGG 0.263158 6.3:+66314580 MS.gene042849:CDS
ATGAACCCTGCACTTGCTTT+TGG 0.268743 6.3:+66315380 MS.gene042849:CDS
GAAGGCTCTGGCCTTGCTTT+GGG 0.287910 6.3:+66314064 MS.gene042849:CDS
CTTGTTGGTGGTCCATTTGA+TGG 0.299715 6.3:+66315350 MS.gene042849:CDS
GGCTCCAATAGTGCCCCTTT+TGG 0.299772 6.3:-66315285 None:intergenic
AGAAGGCTCTGGCCTTGCTT+TGG 0.312813 6.3:+66314063 MS.gene042849:CDS
CTTGAGATTATCATGACATT+TGG 0.328426 6.3:+66315227 MS.gene042849:CDS
GCAACTCGTAGGTATGCTTT+TGG 0.330854 6.3:+66313956 MS.gene042849:CDS
GTAGGTAACTAGTAATCTTC+AGG 0.345529 6.3:-66315542 None:intergenic
TTTCATCTGGCAAGGGGAAT+TGG 0.349294 6.3:+66315185 MS.gene042849:CDS
GTCTTTGCTGGAGAAGGCTC+TGG 0.351672 6.3:+66314052 MS.gene042849:CDS
ATCTTTGTCTTTGCTGGAGA+AGG 0.361094 6.3:+66314046 MS.gene042849:CDS
GATCCCAAAAGGGGCACTAT+TGG 0.365740 6.3:+66315281 MS.gene042849:CDS
GCCATTGCACCTTTAGCAAT+TGG 0.368504 6.3:+66315305 MS.gene042849:CDS
TTGTGGCTGCCCTGTTGCTT+AGG 0.375709 6.3:+66314625 MS.gene042849:CDS
TCTTCCAGCATGCATGTATC+TGG 0.391009 6.3:+66314501 MS.gene042849:CDS
TTTCTCAGCTGGTGAATTGT+TGG 0.394785 6.3:+66314437 MS.gene042849:CDS
TAGTGACAAGCCTAAGCAAC+AGG 0.398970 6.3:-66314635 None:intergenic
CATCTCCATCCTACCAAAGA+AGG 0.428978 6.3:-66315404 None:intergenic
AAGGTAGCTCTAATAGAATC+TGG 0.430438 6.3:-66314001 None:intergenic
CAACTGCAATTGATCCCAAA+AGG 0.437356 6.3:+66315270 MS.gene042849:CDS
GTGACATTTGGTGCTCTCAT+AGG 0.439857 6.3:+66314543 MS.gene042849:CDS
GTCAACCCAGCAGTGACATT+TGG 0.440018 6.3:+66314531 MS.gene042849:CDS
TTCATGCATGCTCCATCAAA+TGG 0.445092 6.3:-66315362 None:intergenic
TCCATTCATTGGTGCAGCAT+TGG 0.446273 6.3:+66315454 MS.gene042849:CDS
TTAGCAATTGGACTCATTGT+TGG 0.449771 6.3:+66315317 MS.gene042849:CDS
TTGGCCCTTCTTTGGTAGGA+TGG 0.450021 6.3:+66315399 MS.gene042849:CDS
GTTGGTGCAAATATCCTTGT+TGG 0.459750 6.3:+66315335 MS.gene042849:CDS
CAAGATTCAGCTTTCTCAGC+TGG 0.469730 6.3:+66314426 MS.gene042849:CDS
CTTCTTTGGTAGGATGGAGA+TGG 0.477208 6.3:+66315405 MS.gene042849:CDS
GATGGAGATGGCACCACCAC+TGG 0.482552 6.3:+66315417 MS.gene042849:CDS
TGAATTGTTGGCAGTTGCAC+TGG 0.483624 6.3:+66314449 MS.gene042849:CDS
AGAGCACCAAATGTCACTGC+TGG 0.484887 6.3:-66314537 None:intergenic
CAGCTGAGAAAGCTGAATCT+TGG 0.491187 6.3:-66314425 None:intergenic
TGTGCATGAGGTGGCTCAGT+TGG 0.498129 6.3:-66315506 None:intergenic
AACTGCAATTGATCCCAAAA+GGG 0.498988 6.3:+66315271 MS.gene042849:CDS
GCCAATGCTGCACCAATGAA+TGG 0.500062 6.3:-66315455 None:intergenic
GAAGGGCCAAAAGCAAGTGC+AGG 0.506585 6.3:-66315386 None:intergenic
GTGCATGAGGTGGCTCAGTT+GGG 0.508449 6.3:-66315505 None:intergenic
AGCAAAGACAAAGATGAAAG+TGG 0.508555 6.3:-66314038 None:intergenic
CTTGCCAGATGAAACCCTCC+TGG 0.524847 6.3:-66315176 None:intergenic
AATCTTCAGGAGCAAGAGGC+TGG 0.526932 6.3:-66315529 None:intergenic
GAGACCAGGAGGGTTTCATC+TGG 0.538168 6.3:+66315172 MS.gene042849:intron
TCAACTTCTAGGTGCTATTG+TGG 0.546373 6.3:+66314608 MS.gene042849:CDS
AGGGGAATTGGTGTAGGACA+TGG 0.549780 6.3:+66315197 MS.gene042849:CDS
GCCACCAGATACATGCATGC+TGG 0.551978 6.3:-66314505 None:intergenic
CAGGAGGGTTTCATCTGGCA+AGG 0.560195 6.3:+66315177 MS.gene042849:CDS
TTCTGTTTATTATAGAGACC+AGG 0.564245 6.3:+66315158 MS.gene042849:intron
TCCAATTGCTAAAGGTGCAA+TGG 0.570541 6.3:-66315306 None:intergenic
GGTGCAAATATCCTTGTTGG+TGG 0.574728 6.3:+66315338 MS.gene042849:CDS
TAGTAATCTTCAGGAGCAAG+AGG 0.578113 6.3:-66315533 None:intergenic
AGTGGAAGCAAATTCAGCTA+AGG 0.584032 6.3:-66314020 None:intergenic
CTGGCAAGGGGAATTGGTGT+AGG 0.587924 6.3:+66315191 MS.gene042849:CDS
TCCAGCATGCATGTATCTGG+TGG 0.589012 6.3:+66314504 MS.gene042849:CDS
ACAATGAGTCCAATTGCTAA+AGG 0.602275 6.3:-66315314 None:intergenic
ATGTCACTGCTGGGTTGACA+TGG 0.602359 6.3:-66314527 None:intergenic
ACATTTGGTGCTCTCATAGG+TGG 0.607002 6.3:+66314546 MS.gene042849:CDS
AGGAGGGTTTCATCTGGCAA+GGG 0.609867 6.3:+66315178 MS.gene042849:CDS
TTGGTGCTCTCATAGGTGGT+AGG 0.611918 6.3:+66314550 MS.gene042849:CDS
TAGCTCTAATAGAATCTGGA+TGG 0.613359 6.3:-66313997 None:intergenic
GGAGGGTTTCATCTGGCAAG+GGG 0.615421 6.3:+66315179 MS.gene042849:CDS
CATCAAATGGACCACCAACA+AGG 0.619845 6.3:-66315349 None:intergenic
ACACTTTGATGGCAACTCGT+AGG 0.632452 6.3:+66313945 None:intergenic
AAGGGCCAAAAGCAAGTGCA+GGG 0.633175 6.3:-66315385 None:intergenic
AGAGGCTGGTGTGCATGAGG+TGG 0.640577 6.3:-66315515 None:intergenic
GTGTATACAAACCCAAAGCA+AGG 0.641996 6.3:-66314075 None:intergenic
ATCTCCATCCTACCAAAGAA+GGG 0.643396 6.3:-66315403 None:intergenic
ACTGCAATTGATCCCAAAAG+GGG 0.644013 6.3:+66315272 MS.gene042849:CDS
GACCAACCCAAAAGATCCAG+TGG 0.644182 6.3:-66315433 None:intergenic
TGTTTATTATAGAGACCAGG+AGG 0.648170 6.3:+66315161 MS.gene042849:intron
TAGGCTTGTCACTAATAACA+TGG 0.665439 6.3:+66314644 MS.gene042849:CDS
GCAAGAGGCTGGTGTGCATG+AGG 0.666450 6.3:-66315518 None:intergenic
AGTGACAAGCCTAAGCAACA+GGG 0.669493 6.3:-66314634 None:intergenic
GTTTATTATAGAGACCAGGA+GGG 0.672843 6.3:+66315162 MS.gene042849:intron
GAGCACCAAATGTCACTGCT+GGG 0.683403 6.3:-66314536 None:intergenic
CAACCCAAAAGATCCAGTGG+TGG 0.714046 6.3:-66315430 None:intergenic
TCCAATAGTAGACAGCACGA+AGG 0.754250 6.3:-66314581 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAACATGATTGAAAAAAA+TGG + chr6.3:66315103-66315122 MS.gene042849:intron 15.0%
!! GTTAATTAATTATGCAACTA+AGG - chr6.3:66314116-66314135 None:intergenic 20.0%
!! TATCAAATTATTAGCAATGT+CGG - chr6.3:66314184-66314203 None:intergenic 20.0%
!!! TATCATAGACTCATTTTTAA+TGG + chr6.3:66314808-66314827 MS.gene042849:intron 20.0%
! AGAAAGAGTCATCATAATTT+AGG + chr6.3:66314717-66314736 MS.gene042849:intron 25.0%
! AGATCTAACATATTCAAAAG+AGG - chr6.3:66314771-66314790 None:intergenic 25.0%
! TTCAGTGTAATTATATGTGT+CGG + chr6.3:66314215-66314234 MS.gene042849:intron 25.0%
!! ATTTTCAATAAGAACTCATG+TGG + chr6.3:66315126-66315145 MS.gene042849:intron 25.0%
AAGAGTCATCATAATTTAGG+AGG + chr6.3:66314720-66314739 MS.gene042849:intron 30.0%
CTTCATATTAAAGACATGTC+AGG + chr6.3:66314979-66314998 MS.gene042849:intron 30.0%
CTTGAGATTATCATGACATT+TGG + chr6.3:66315227-66315246 MS.gene042849:CDS 30.0%
GAATATGTTAGATCTGAGTT+TGG + chr6.3:66314776-66314795 MS.gene042849:intron 30.0%
TTCTGTTTATTATAGAGACC+AGG + chr6.3:66315158-66315177 MS.gene042849:intron 30.0%
! AACATATTCAAAAGAGGCAT+CGG - chr6.3:66314765-66314784 None:intergenic 30.0%
!! AATAGGCTTTAAAGAGTTTC+AGG - chr6.3:66314868-66314887 None:intergenic 30.0%
AACTGCAATTGATCCCAAAA+GGG + chr6.3:66315271-66315290 MS.gene042849:CDS 35.0%
ACAATGAGTCCAATTGCTAA+AGG - chr6.3:66315317-66315336 None:intergenic 35.0%
ACACCAACACTTCAGATTAA+AGG + chr6.3:66314920-66314939 MS.gene042849:intron 35.0%
ACCTCATTGAATCTTTGACA+AGG + chr6.3:66314842-66314861 MS.gene042849:intron 35.0%
ACTTTCATCTTTGTCTTTGC+TGG + chr6.3:66314040-66314059 MS.gene042849:CDS 35.0%
AGCAAAGACAAAGATGAAAG+TGG - chr6.3:66314041-66314060 None:intergenic 35.0%
GTCATCATAATTTAGGAGGA+AGG + chr6.3:66314724-66314743 MS.gene042849:intron 35.0%
GTTTATTATAGAGACCAGGA+GGG + chr6.3:66315162-66315181 MS.gene042849:intron 35.0%
TACCACATAGCTATGAAACA+CGG - chr6.3:66314304-66314323 None:intergenic 35.0%
TAGGCTTGTCACTAATAACA+TGG + chr6.3:66314644-66314663 MS.gene042849:CDS 35.0%
TATTGGATTGCTCAACTTCT+AGG + chr6.3:66314597-66314616 MS.gene042849:CDS 35.0%
TGTTTATTATAGAGACCAGG+AGG + chr6.3:66315161-66315180 MS.gene042849:intron 35.0%
TTAGCAATTGGACTCATTGT+TGG + chr6.3:66315317-66315336 MS.gene042849:CDS 35.0%
! ACACCTTTAATCTGAAGTGT+TGG - chr6.3:66314926-66314945 None:intergenic 35.0%
! TAAGAACTCATGTGGTGTTA+TGG + chr6.3:66315134-66315153 MS.gene042849:intron 35.0%
!! AAGGTAGCTCTAATAGAATC+TGG - chr6.3:66314004-66314023 None:intergenic 35.0%
!! TAGCTCTAATAGAATCTGGA+TGG - chr6.3:66314000-66314019 None:intergenic 35.0%
ACTGCAATTGATCCCAAAAG+GGG + chr6.3:66315272-66315291 MS.gene042849:CDS 40.0%
AGTGGAAGCAAATTCAGCTA+AGG - chr6.3:66314023-66314042 None:intergenic 40.0%
ATCTCCATCCTACCAAAGAA+GGG - chr6.3:66315406-66315425 None:intergenic 40.0%
ATCTTTGTCTTTGCTGGAGA+AGG + chr6.3:66314046-66314065 MS.gene042849:CDS 40.0%
CAACTGCAATTGATCCCAAA+AGG + chr6.3:66315270-66315289 MS.gene042849:CDS 40.0%
GCCTTGTCAAAGATTCAATG+AGG - chr6.3:66314846-66314865 None:intergenic 40.0%
GGCATGGATTCGTGAATAAT+AGG - chr6.3:66314885-66314904 None:intergenic 40.0%
GTGTATACAAACCCAAAGCA+AGG - chr6.3:66314078-66314097 None:intergenic 40.0%
GTTGGTGCAAATATCCTTGT+TGG + chr6.3:66315335-66315354 MS.gene042849:CDS 40.0%
TAGTAATCTTCAGGAGCAAG+AGG - chr6.3:66315536-66315555 None:intergenic 40.0%
TTCATGCATGCTCCATCAAA+TGG - chr6.3:66315365-66315384 None:intergenic 40.0%
TTTAGGAGGAAGGTGAGAAT+AGG + chr6.3:66314734-66314753 MS.gene042849:intron 40.0%
TTTCTCAGCTGGTGAATTGT+TGG + chr6.3:66314437-66314456 MS.gene042849:CDS 40.0%
! CATGTCAGGTGTCAGTTTTT+CGG + chr6.3:66314993-66315012 MS.gene042849:intron 40.0%
! TCCAATTGCTAAAGGTGCAA+TGG - chr6.3:66315309-66315328 None:intergenic 40.0%
!! TATATGTGTCGGTGTTGTGT+CGG + chr6.3:66314226-66314245 MS.gene042849:intron 40.0%
!! TCAACTTCTAGGTGCTATTG+TGG + chr6.3:66314608-66314627 MS.gene042849:CDS 40.0%
!! TCGTAGGTATGCTTTTGGAA+GGG + chr6.3:66313961-66313980 MS.gene042849:CDS 40.0%
!!! TTTTGGGTTGGTCCATTCAT+TGG + chr6.3:66315443-66315462 MS.gene042849:CDS 40.0%
ACCAGCATGTCATGACTATG+TGG + chr6.3:66314134-66314153 MS.gene042849:intron 45.0%
AGTGACAAGCCTAAGCAACA+GGG - chr6.3:66314637-66314656 None:intergenic 45.0%
CAAGATTCAGCTTTCTCAGC+TGG + chr6.3:66314426-66314445 MS.gene042849:CDS 45.0%
CAGCTGAGAAAGCTGAATCT+TGG - chr6.3:66314428-66314447 None:intergenic 45.0%
CATCAAATGGACCACCAACA+AGG - chr6.3:66315352-66315371 None:intergenic 45.0%
CATCTCCATCCTACCAAAGA+AGG - chr6.3:66315407-66315426 None:intergenic 45.0%
CTTCTTTGGTAGGATGGAGA+TGG + chr6.3:66315405-66315424 MS.gene042849:CDS 45.0%
GACTATGTGGCTATGAAGCA+CGG + chr6.3:66314147-66314166 MS.gene042849:intron 45.0%
GAGGAAGGTGAGAATAGGTT+AGG + chr6.3:66314739-66314758 MS.gene042849:intron 45.0%
GCCATTGCACCTTTAGCAAT+TGG + chr6.3:66315305-66315324 MS.gene042849:CDS 45.0%
GGTGCAAATATCCTTGTTGG+TGG + chr6.3:66315338-66315357 MS.gene042849:CDS 45.0%
GTCCGTGTTTCATAGCTATG+TGG + chr6.3:66314299-66314318 MS.gene042849:intron 45.0%
TAGTGACAAGCCTAAGCAAC+AGG - chr6.3:66314638-66314657 None:intergenic 45.0%
TCCAATAGTAGACAGCACGA+AGG - chr6.3:66314584-66314603 None:intergenic 45.0%
TCCATTCATTGGTGCAGCAT+TGG + chr6.3:66315454-66315473 MS.gene042849:CDS 45.0%
TCCTTCGTGCTGTCTACTAT+TGG + chr6.3:66314580-66314599 MS.gene042849:CDS 45.0%
TCTTCCAGCATGCATGTATC+TGG + chr6.3:66314501-66314520 MS.gene042849:CDS 45.0%
TGAATTGTTGGCAGTTGCAC+TGG + chr6.3:66314449-66314468 MS.gene042849:CDS 45.0%
TTTCATCTGGCAAGGGGAAT+TGG + chr6.3:66315185-66315204 MS.gene042849:CDS 45.0%
! ATGAACCCTGCACTTGCTTT+TGG + chr6.3:66315380-66315399 MS.gene042849:CDS 45.0%
! CTCGTAGGTATGCTTTTGGA+AGG + chr6.3:66313960-66313979 MS.gene042849:CDS 45.0%
! GCAACTCGTAGGTATGCTTT+TGG + chr6.3:66313956-66313975 MS.gene042849:CDS 45.0%
!! ACATTTGGTGCTCTCATAGG+TGG + chr6.3:66314546-66314565 MS.gene042849:CDS 45.0%
!! ACTTGCTTTTGGCCCTTCTT+TGG + chr6.3:66315391-66315410 MS.gene042849:CDS 45.0%
!! CTTGTTGGTGGTCCATTTGA+TGG + chr6.3:66315350-66315369 MS.gene042849:CDS 45.0%
!! GAAGTGTTGGTGTCGAACAA+TGG - chr6.3:66314913-66314932 None:intergenic 45.0%
!! GATTAGAGGTGTCCTGGTAT+CGG - chr6.3:66314271-66314290 None:intergenic 45.0%
!! GTGACATTTGGTGCTCTCAT+AGG + chr6.3:66314543-66314562 MS.gene042849:CDS 45.0%
AAGGGCCAAAAGCAAGTGCA+GGG - chr6.3:66315388-66315407 None:intergenic 50.0%
AATCTTCAGGAGCAAGAGGC+TGG - chr6.3:66315532-66315551 None:intergenic 50.0%
ACCAGGACACCTCTAATCTG+AGG + chr6.3:66314273-66314292 MS.gene042849:intron 50.0%
ACGGACACTCCTCAGATTAG+AGG - chr6.3:66314285-66314304 None:intergenic 50.0%
AGGAGGGTTTCATCTGGCAA+GGG + chr6.3:66315178-66315197 MS.gene042849:CDS 50.0%
AGGGGAATTGGTGTAGGACA+TGG + chr6.3:66315197-66315216 MS.gene042849:CDS 50.0%
CAACCCAAAAGATCCAGTGG+TGG - chr6.3:66315433-66315452 None:intergenic 50.0%
GACCAACCCAAAAGATCCAG+TGG - chr6.3:66315436-66315455 None:intergenic 50.0%
GAGCACCAAATGTCACTGCT+GGG - chr6.3:66314539-66314558 None:intergenic 50.0%
GCCAATGCTGCACCAATGAA+TGG - chr6.3:66315458-66315477 None:intergenic 50.0%
GCCACATAGTCATGACATGC+TGG - chr6.3:66314138-66314157 None:intergenic 50.0%
GTCAACCCAGCAGTGACATT+TGG + chr6.3:66314531-66314550 MS.gene042849:CDS 50.0%
TACGACACATGTCCGATACC+AGG + chr6.3:66314256-66314275 MS.gene042849:intron 50.0%
TCCAGCATGCATGTATCTGG+TGG + chr6.3:66314504-66314523 MS.gene042849:CDS 50.0%
TTGGCCCTTCTTTGGTAGGA+TGG + chr6.3:66315399-66315418 MS.gene042849:CDS 50.0%
! AGAGCACCAAATGTCACTGC+TGG - chr6.3:66314540-66314559 None:intergenic 50.0%
! ATGTCACTGCTGGGTTGACA+TGG - chr6.3:66314530-66314549 None:intergenic 50.0%
! GCACCACCACTGGATCTTTT+GGG + chr6.3:66315427-66315446 MS.gene042849:CDS 50.0%
! GCTCCAATAGTGCCCCTTTT+GGG - chr6.3:66315287-66315306 None:intergenic 50.0%
! GCTTTTGGCCCTTCTTTGGT+AGG + chr6.3:66315395-66315414 MS.gene042849:CDS 50.0%
! TCGAACAATGGTGTCAGGCA+TGG - chr6.3:66314901-66314920 None:intergenic 50.0%
! TGGTGTCGAACAATGGTGTC+AGG - chr6.3:66314906-66314925 None:intergenic 50.0%
! TTGGTGCTCTCATAGGTGGT+AGG + chr6.3:66314550-66314569 MS.gene042849:CDS 50.0%
!! CACCACTGGATCTTTTGGGT+TGG + chr6.3:66315431-66315450 MS.gene042849:CDS 50.0%
!! GATCCCAAAAGGGGCACTAT+TGG + chr6.3:66315281-66315300 MS.gene042849:CDS 50.0%
!! TCCTCAGATTAGAGGTGTCC+TGG - chr6.3:66314277-66314296 None:intergenic 50.0%
!! TGCTTTTGGAAGGGCTGATG+AGG + chr6.3:66313970-66313989 MS.gene042849:CDS 50.0%
CAGGAGGGTTTCATCTGGCA+AGG + chr6.3:66315177-66315196 MS.gene042849:CDS 55.0%
CTGGCAAGGGGAATTGGTGT+AGG + chr6.3:66315191-66315210 MS.gene042849:CDS 55.0%
CTTGCCAGATGAAACCCTCC+TGG - chr6.3:66315179-66315198 None:intergenic 55.0%
GAAGGGCCAAAAGCAAGTGC+AGG - chr6.3:66315389-66315408 None:intergenic 55.0%
GAGACCAGGAGGGTTTCATC+TGG + chr6.3:66315172-66315191 MS.gene042849:intron 55.0%
GCCACCAGATACATGCATGC+TGG - chr6.3:66314508-66314527 None:intergenic 55.0%
GGAGGGTTTCATCTGGCAAG+GGG + chr6.3:66315179-66315198 MS.gene042849:CDS 55.0%
GTCTTTGCTGGAGAAGGCTC+TGG + chr6.3:66314052-66314071 MS.gene042849:CDS 55.0%
GTGCATGAGGTGGCTCAGTT+GGG - chr6.3:66315508-66315527 None:intergenic 55.0%
TGTGCATGAGGTGGCTCAGT+TGG - chr6.3:66315509-66315528 None:intergenic 55.0%
! GGCACCACCACTGGATCTTT+TGG + chr6.3:66315426-66315445 MS.gene042849:CDS 55.0%
! GGCTCCAATAGTGCCCCTTT+TGG - chr6.3:66315288-66315307 None:intergenic 55.0%
! TTGTGGCTGCCCTGTTGCTT+AGG + chr6.3:66314625-66314644 MS.gene042849:CDS 55.0%
!! AGAAGGCTCTGGCCTTGCTT+TGG + chr6.3:66314063-66314082 MS.gene042849:CDS 55.0%
!! GAAGGCTCTGGCCTTGCTTT+GGG + chr6.3:66314064-66314083 MS.gene042849:CDS 55.0%
AGAGGCTGGTGTGCATGAGG+TGG - chr6.3:66315518-66315537 None:intergenic 60.0%
GATGGAGATGGCACCACCAC+TGG + chr6.3:66315417-66315436 MS.gene042849:CDS 60.0%
GCAAGAGGCTGGTGTGCATG+AGG - chr6.3:66315521-66315540 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 66313953 66315556 66313953 ID=MS.gene042849
chr6.3 mRNA 66313953 66315556 66313953 ID=MS.gene042849.t1;Parent=MS.gene042849
chr6.3 exon 66313953 66314085 66313953 ID=MS.gene042849.t1.exon1;Parent=MS.gene042849.t1
chr6.3 CDS 66313953 66314085 66313953 ID=cds.MS.gene042849.t1;Parent=MS.gene042849.t1
chr6.3 exon 66314415 66314665 66314415 ID=MS.gene042849.t1.exon2;Parent=MS.gene042849.t1
chr6.3 CDS 66314415 66314665 66314415 ID=cds.MS.gene042849.t1;Parent=MS.gene042849.t1
chr6.3 exon 66315173 66315556 66315173 ID=MS.gene042849.t1.exon3;Parent=MS.gene042849.t1
chr6.3 CDS 66315173 66315556 66315173 ID=cds.MS.gene042849.t1;Parent=MS.gene042849.t1
Gene Sequence

>MS.gene042849

ATGGCAACTCGTAGGTATGCTTTTGGAAGGGCTGATGAGGTTTACCATCCAGATTCTATTAGAGCTACCTTAGCTGAATTTGCTTCCACTTTCATCTTTGTCTTTGCTGGAGAAGGCTCTGGCCTTGCTTTGGGTTTGTATACACTCAAAATTAACATAACCTTAGTTGCATAATTAATTAACCAGCATGTCATGACTATGTGGCTATGAAGCACGGATACTGACACGCCGACATTGCTAATAATTTGATAAAATCTCATAATTCAGTGTAATTATATGTGTCGGTGTTGTGTCGGTGTTAGATACGACACATGTCCGATACCAGGACACCTCTAATCTGAGGAGTGTCCGTGTTTCATAGCTATGTGGTAGTTAACATTTGTGTTTATTTTTTATGCATATATATATATATATTATAGTATGTTGATTGTGATTTTTGTGCATTGTTTCTCTTTGTTACAGTTAAGATATACCAAGATTCAGCTTTCTCAGCTGGTGAATTGTTGGCAGTTGCACTGGCACATGCATTTGCTCTATTTGCTGCTGTGTCTTCCAGCATGCATGTATCTGGTGGCCATGTCAACCCAGCAGTGACATTTGGTGCTCTCATAGGTGGTAGGATCTCTGTCCTTCGTGCTGTCTACTATTGGATTGCTCAACTTCTAGGTGCTATTGTGGCTGCCCTGTTGCTTAGGCTTGTCACTAATAACATGGTAATCATTTTTTTGATGAATGAGTCATAATTTTTATCATGTGTATCAGATAGAAAGAGTCATCATAATTTAGGAGGAAGGTGAGAATAGGTTAGGCCGATGCCTCTTTTGAATATGTTAGATCTGAGTTTGGTCTATCATCTATCATAGACTCATTTTTAATGGCGAGTTGTCTGACCTCATTGAATCTTTGACAAGGCCTGAAACTCTTTAAAGCCTATTATTCACGAATCCATGCCTGACACCATTGTTCGACACCAACACTTCAGATTAAAGGTGTCACATGCGTGAATGTACATACAAGTATCTGACACTTCATATTAAAGACATGTCAGGTGTCAGTTTTTCGGCTTGTCTATGTCAATACATCATAGAAAATGTAGTAAAAAAAGATTAGATAATATTAACATGATTGAAAACAATAGATTTTCTATAATATTAACATGATTGAAAAAAATGGATTTTCAATAAGAACTCATGTGGTGTTATGGTTTCTGTTTATTATAGAGACCAGGAGGGTTTCATCTGGCAAGGGGAATTGGTGTAGGACATGGACTTATACTTGAGATTATCATGACATTTGGACTAATGTACACAGTATATGCAACTGCAATTGATCCCAAAAGGGGCACTATTGGAGCCATTGCACCTTTAGCAATTGGACTCATTGTTGGTGCAAATATCCTTGTTGGTGGTCCATTTGATGGAGCATGCATGAACCCTGCACTTGCTTTTGGCCCTTCTTTGGTAGGATGGAGATGGCACCACCACTGGATCTTTTGGGTTGGTCCATTCATTGGTGCAGCATTGGCAGCACTCATATATGAATATCTTGTGATCCCAACTGAGCCACCTCATGCACACCAGCCTCTTGCTCCTGAAGATTACTAG
Protein sequence

>MS.gene042849.t1

MATRRYAFGRADEVYHPDSIRATLAEFASTFIFVFAGEGSGLALVKIYQDSAFSAGELLAVALAHAFALFAAVSSSMHVSGGHVNPAVTFGALIGGRISVLRAVYYWIAQLLGAIVAALLLRLVTNNMRPGGFHLARGIGVGHGLILEIIMTFGLMYTVYATAIDPKRGTIGAIAPLAIGLIVGANILVGGPFDGACMNPALAFGPSLVGWRWHHHWIFWVGPFIGAALAALIYEYLVIPTEPPHAHQPLAPEDY