Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71833.t1 | XP_013470181.1 | 96.3 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 5.80E-235 | 823.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71833.t1 | Q8RWD0 | 45.3 | 446 | 192 | 12 | 6 | 434 | 3 | 413 | 2.6e-82 | 307.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71833.t1 | A0A072VQB9 | 96.3 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 4.2e-235 | 823.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene71833.t1 | TF | C2C2-CO-like |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049260 | MS.gene71833 | 0.803075 | 4.14E-49 | -1.69E-46 |
| MS.gene049297 | MS.gene71833 | 0.830629 | 2.64E-55 | -1.69E-46 |
| MS.gene049346 | MS.gene71833 | 0.858311 | 8.99E-63 | -1.69E-46 |
| MS.gene049577 | MS.gene71833 | 0.813625 | 2.33E-51 | -1.69E-46 |
| MS.gene049758 | MS.gene71833 | 0.847034 | 1.49E-59 | -1.69E-46 |
| MS.gene049910 | MS.gene71833 | 0.811779 | 5.90E-51 | -1.69E-46 |
| MS.gene049993 | MS.gene71833 | 0.800404 | 1.46E-48 | -1.69E-46 |
| MS.gene050076 | MS.gene71833 | 0.830196 | 3.37E-55 | -1.69E-46 |
| MS.gene050128 | MS.gene71833 | 0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene050301 | MS.gene71833 | 0.843203 | 1.62E-58 | -1.69E-46 |
| MS.gene050397 | MS.gene71833 | 0.814689 | 1.36E-51 | -1.69E-46 |
| MS.gene050404 | MS.gene71833 | 0.8527 | 3.89E-61 | -1.69E-46 |
| MS.gene050406 | MS.gene71833 | 0.875482 | 2.98E-68 | -1.69E-46 |
| MS.gene050464 | MS.gene71833 | 0.824135 | 9.51E-54 | -1.69E-46 |
| MS.gene050529 | MS.gene71833 | 0.814043 | 1.88E-51 | -1.69E-46 |
| MS.gene050558 | MS.gene71833 | 0.801542 | 8.57E-49 | -1.69E-46 |
| MS.gene050642 | MS.gene71833 | 0.82723 | 1.75E-54 | -1.69E-46 |
| MS.gene05101 | MS.gene71833 | 0.826678 | 2.38E-54 | -1.69E-46 |
| MS.gene05119 | MS.gene71833 | -0.808286 | 3.34E-50 | -1.69E-46 |
| MS.gene051240 | MS.gene71833 | 0.808018 | 3.81E-50 | -1.69E-46 |
| MS.gene05137 | MS.gene71833 | 0.821227 | 4.52E-53 | -1.69E-46 |
| MS.gene051471 | MS.gene71833 | 0.840768 | 7.13E-58 | -1.69E-46 |
| MS.gene051582 | MS.gene71833 | 0.834713 | 2.56E-56 | -1.69E-46 |
| MS.gene051662 | MS.gene71833 | 0.844628 | 6.72E-59 | -1.69E-46 |
| MS.gene051663 | MS.gene71833 | 0.815792 | 7.71E-52 | -1.69E-46 |
| MS.gene051860 | MS.gene71833 | 0.800417 | 1.46E-48 | -1.69E-46 |
| MS.gene051983 | MS.gene71833 | 0.819397 | 1.19E-52 | -1.69E-46 |
| MS.gene052078 | MS.gene71833 | 0.82956 | 4.81E-55 | -1.69E-46 |
| MS.gene052176 | MS.gene71833 | 0.801187 | 1.01E-48 | -1.69E-46 |
| MS.gene052178 | MS.gene71833 | 0.833725 | 4.52E-56 | -1.69E-46 |
| MS.gene052253 | MS.gene71833 | 0.803514 | 3.36E-49 | -1.69E-46 |
| MS.gene052302 | MS.gene71833 | 0.802153 | 6.42E-49 | -1.69E-46 |
| MS.gene052309 | MS.gene71833 | -0.806433 | 8.24E-50 | -1.69E-46 |
| MS.gene052326 | MS.gene71833 | 0.807016 | 6.21E-50 | -1.69E-46 |
| MS.gene052340 | MS.gene71833 | 0.819184 | 1.33E-52 | -1.69E-46 |
| MS.gene052376 | MS.gene71833 | 0.805421 | 1.35E-49 | -1.69E-46 |
| MS.gene053073 | MS.gene71833 | 0.80242 | 5.66E-49 | -1.69E-46 |
| MS.gene053557 | MS.gene71833 | 0.80568 | 1.19E-49 | -1.69E-46 |
| MS.gene053836 | MS.gene71833 | 0.890498 | 9.33E-74 | -1.69E-46 |
| MS.gene053907 | MS.gene71833 | 0.878532 | 2.60E-69 | -1.69E-46 |
| MS.gene053971 | MS.gene71833 | 0.866215 | 3.36E-65 | -1.69E-46 |
| MS.gene054183 | MS.gene71833 | 0.80168 | 8.03E-49 | -1.69E-46 |
| MS.gene054473 | MS.gene71833 | 0.857969 | 1.14E-62 | -1.69E-46 |
| MS.gene054618 | MS.gene71833 | 0.804974 | 1.67E-49 | -1.69E-46 |
| MS.gene054930 | MS.gene71833 | 0.81451 | 1.49E-51 | -1.69E-46 |
| MS.gene054942 | MS.gene71833 | 0.827693 | 1.36E-54 | -1.69E-46 |
| MS.gene054983 | MS.gene71833 | 0.873656 | 1.24E-67 | -1.69E-46 |
| MS.gene055277 | MS.gene71833 | 0.82885 | 7.15E-55 | -1.69E-46 |
| MS.gene055422 | MS.gene71833 | 0.848268 | 6.83E-60 | -1.69E-46 |
| MS.gene055428 | MS.gene71833 | 0.874133 | 8.57E-68 | -1.69E-46 |
| MS.gene05553 | MS.gene71833 | 0.812229 | 4.71E-51 | -1.69E-46 |
| MS.gene055604 | MS.gene71833 | 0.802204 | 6.27E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene71833 | 0.839761 | 1.31E-57 | -1.69E-46 |
| MS.gene055980 | MS.gene71833 | 0.821044 | 4.98E-53 | -1.69E-46 |
| MS.gene056451 | MS.gene71833 | 0.871015 | 9.42E-67 | -1.69E-46 |
| MS.gene056612 | MS.gene71833 | 0.865742 | 4.74E-65 | -1.69E-46 |
| MS.gene056723 | MS.gene71833 | 0.805747 | 1.15E-49 | -1.69E-46 |
| MS.gene056772 | MS.gene71833 | 0.859711 | 3.42E-63 | -1.69E-46 |
| MS.gene056899 | MS.gene71833 | -0.833429 | 5.36E-56 | -1.69E-46 |
| MS.gene056979 | MS.gene71833 | 0.812838 | 3.47E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene71833 | 0.869633 | 2.68E-66 | -1.69E-46 |
| MS.gene057121 | MS.gene71833 | 0.838467 | 2.83E-57 | -1.69E-46 |
| MS.gene057245 | MS.gene71833 | 0.847866 | 8.82E-60 | -1.69E-46 |
| MS.gene05733 | MS.gene71833 | 0.80249 | 5.47E-49 | -1.69E-46 |
| MS.gene057439 | MS.gene71833 | 0.852414 | 4.69E-61 | -1.69E-46 |
| MS.gene057639 | MS.gene71833 | 0.829735 | 4.36E-55 | -1.69E-46 |
| MS.gene058002 | MS.gene71833 | 0.857582 | 1.48E-62 | -1.69E-46 |
| MS.gene05811 | MS.gene71833 | 0.803966 | 2.71E-49 | -1.69E-46 |
| MS.gene058142 | MS.gene71833 | 0.837173 | 6.08E-57 | -1.69E-46 |
| MS.gene058201 | MS.gene71833 | 0.807621 | 4.62E-50 | -1.69E-46 |
| MS.gene058233 | MS.gene71833 | 0.845284 | 4.47E-59 | -1.69E-46 |
| MS.gene058276 | MS.gene71833 | 0.823066 | 1.69E-53 | -1.69E-46 |
| MS.gene058660 | MS.gene71833 | 0.809903 | 1.50E-50 | -1.69E-46 |
| MS.gene058746 | MS.gene71833 | 0.803966 | 2.71E-49 | -1.69E-46 |
| MS.gene058749 | MS.gene71833 | 0.82788 | 1.23E-54 | -1.69E-46 |
| MS.gene058845 | MS.gene71833 | 0.805585 | 1.24E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene71833 | 0.823804 | 1.14E-53 | -1.69E-46 |
| MS.gene059228 | MS.gene71833 | 0.800244 | 1.58E-48 | -1.69E-46 |
| MS.gene059518 | MS.gene71833 | 0.823007 | 1.75E-53 | -1.69E-46 |
| MS.gene059597 | MS.gene71833 | 0.809309 | 2.02E-50 | -1.69E-46 |
| MS.gene05984 | MS.gene71833 | 0.818985 | 1.47E-52 | -1.69E-46 |
| MS.gene059894 | MS.gene71833 | 0.883088 | 6.03E-71 | -1.69E-46 |
| MS.gene059899 | MS.gene71833 | 0.813177 | 2.92E-51 | -1.69E-46 |
| MS.gene06037 | MS.gene71833 | 0.834045 | 3.76E-56 | -1.69E-46 |
| MS.gene060407 | MS.gene71833 | 0.821075 | 4.90E-53 | -1.69E-46 |
| MS.gene060476 | MS.gene71833 | 0.804827 | 1.79E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene71833 | 0.896179 | 4.73E-76 | -1.69E-46 |
| MS.gene061112 | MS.gene71833 | 0.864756 | 9.67E-65 | -1.69E-46 |
| MS.gene061113 | MS.gene71833 | 0.814283 | 1.67E-51 | -1.69E-46 |
| MS.gene061114 | MS.gene71833 | -0.805033 | 1.62E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene71833 | 0.861058 | 1.34E-63 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71833.t1 | MTR_1g110870 | 96.330 | 436 | 16 | 0 | 1 | 436 | 1 | 436 | 0.0 | 872 |
| MS.gene71833.t1 | MTR_8g104190 | 41.372 | 452 | 188 | 18 | 9 | 433 | 11 | 412 | 3.41e-82 | 259 |
| MS.gene71833.t1 | MTR_5g072780 | 44.144 | 111 | 42 | 3 | 316 | 424 | 124 | 216 | 6.17e-19 | 85.5 |
| MS.gene71833.t1 | MTR_7g032240 | 40.152 | 132 | 56 | 4 | 316 | 424 | 220 | 351 | 5.99e-17 | 82.4 |
| MS.gene71833.t1 | MTR_4g127420 | 44.898 | 98 | 42 | 4 | 321 | 418 | 293 | 378 | 1.13e-13 | 72.8 |
| MS.gene71833.t1 | MTR_1g013450 | 47.561 | 82 | 36 | 2 | 351 | 427 | 221 | 300 | 2.58e-12 | 67.8 |
| MS.gene71833.t1 | MTR_3g105710 | 63.636 | 55 | 16 | 1 | 370 | 424 | 224 | 274 | 4.79e-12 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene71833.t1 | AT1G25440 | 45.622 | 434 | 188 | 10 | 6 | 424 | 3 | 403 | 6.99e-103 | 312 |
| MS.gene71833.t1 | AT1G68520 | 44.018 | 443 | 188 | 16 | 6 | 429 | 3 | 404 | 2.98e-91 | 282 |
| MS.gene71833.t1 | AT1G73870 | 38.149 | 443 | 190 | 19 | 8 | 427 | 9 | 390 | 3.91e-65 | 214 |
| MS.gene71833.t1 | AT4G25990 | 49.533 | 107 | 38 | 3 | 318 | 424 | 293 | 383 | 8.90e-22 | 97.1 |
| MS.gene71833.t1 | AT5G57180 | 45.217 | 115 | 43 | 3 | 318 | 431 | 337 | 432 | 2.63e-21 | 96.3 |
| MS.gene71833.t1 | AT1G49130 | 40.164 | 122 | 65 | 3 | 15 | 128 | 23 | 144 | 2.85e-19 | 89.0 |
| MS.gene71833.t1 | AT1G49130 | 45.794 | 107 | 37 | 3 | 319 | 425 | 236 | 321 | 7.24e-17 | 81.6 |
| MS.gene71833.t1 | AT1G49130 | 40.164 | 122 | 65 | 3 | 15 | 128 | 16 | 137 | 2.98e-19 | 88.6 |
| MS.gene71833.t1 | AT1G49130 | 45.794 | 107 | 37 | 3 | 319 | 425 | 229 | 314 | 9.48e-17 | 81.3 |
| MS.gene71833.t1 | AT4G25990 | 43.443 | 122 | 38 | 4 | 318 | 424 | 293 | 398 | 2.80e-18 | 87.0 |
| MS.gene71833.t1 | AT5G14370 | 38.028 | 142 | 73 | 4 | 291 | 424 | 202 | 336 | 5.04e-18 | 85.5 |
| MS.gene71833.t1 | AT4G25990 | 47.525 | 101 | 37 | 3 | 318 | 418 | 293 | 377 | 5.07e-18 | 85.9 |
| MS.gene71833.t1 | AT1G07050 | 43.750 | 128 | 45 | 5 | 303 | 426 | 91 | 195 | 2.01e-17 | 80.9 |
| MS.gene71833.t1 | AT5G57180 | 45.098 | 102 | 36 | 3 | 318 | 418 | 337 | 419 | 7.99e-17 | 82.4 |
| MS.gene71833.t1 | AT1G07050 | 41.667 | 120 | 43 | 5 | 303 | 418 | 91 | 187 | 5.00e-13 | 68.2 |
| MS.gene71833.t1 | AT5G24930 | 52.113 | 71 | 28 | 1 | 360 | 424 | 267 | 337 | 1.27e-11 | 66.2 |
| MS.gene71833.t1 | AT5G15840 | 52.308 | 65 | 28 | 2 | 1 | 62 | 41 | 105 | 8.40e-11 | 63.9 |
Find 98 sgRNAs with CRISPR-Local
Find 119 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTGGTGTTCTTGCCTTCT+TGG | 0.140899 | 1.3:+75569408 | None:intergenic |
| GAAGAATTCATCACCATTAA+TGG | 0.149936 | 1.3:+75569456 | None:intergenic |
| ACAAATCTCCCTTTCATTCT+TGG | 0.172964 | 1.3:+75568036 | None:intergenic |
| ATTATTTATCAGGGAACTTT+TGG | 0.202916 | 1.3:-75568228 | MS.gene71833:intron |
| TGAAAATAATGATTATAATA+AGG | 0.203891 | 1.3:-75568750 | MS.gene71833:CDS |
| TTCTCATCAGCATCCAAATT+TGG | 0.222169 | 1.3:+75568638 | None:intergenic |
| TTTCATCTTGTGCCGGATTT+AGG | 0.234999 | 1.3:-75569319 | MS.gene71833:CDS |
| TATGGTGAGTTTGGTGGATA+TGG | 0.250845 | 1.3:-75568186 | MS.gene71833:CDS |
| ATGAAGCGCAACTTCTTTAC+AGG | 0.254107 | 1.3:-75569261 | MS.gene71833:CDS |
| TAATGGTGACAAGCCAAATT+TGG | 0.271510 | 1.3:-75568651 | MS.gene71833:CDS |
| CTGCCTCCATCTACCGTTAC+TGG | 0.277791 | 1.3:+75568156 | None:intergenic |
| GAAAATAATGATTATAATAA+GGG | 0.279237 | 1.3:-75568749 | MS.gene71833:CDS |
| GCGTCGTACAAGTCCATTAA+TGG | 0.290118 | 1.3:-75569469 | MS.gene71833:CDS |
| ACGGAGGTTCATTATGCTTA+TGG | 0.290853 | 1.3:-75568204 | MS.gene71833:CDS |
| CTCAACATCAGCTGCAAATT+CGG | 0.302034 | 1.3:+75569027 | None:intergenic |
| GCAACAGAAGGATCGAAAAT+TGG | 0.331767 | 1.3:+75569235 | None:intergenic |
| CATTATGCTTATGGTGAGTT+TGG | 0.336402 | 1.3:-75568195 | MS.gene71833:CDS |
| TTCTTGGTGAAGCCAGAAAA+AGG | 0.344434 | 1.3:+75569424 | None:intergenic |
| GGTTGTTCTCAAAGAAAATA+AGG | 0.347288 | 1.3:-75568092 | MS.gene71833:CDS |
| GATTAGAAAATGAGTGTATA+GGG | 0.361641 | 1.3:-75568988 | MS.gene71833:CDS |
| CATTAGTCCAGGGACACTTT+TGG | 0.361781 | 1.3:+75568669 | None:intergenic |
| AGCTGATGTTGAGAGCTTAT+TGG | 0.374691 | 1.3:-75569017 | MS.gene71833:CDS |
| AGAAAATAAGGTACGAGGTT+AGG | 0.382846 | 1.3:-75568080 | MS.gene71833:CDS |
| GGATTAGAAAATGAGTGTAT+AGG | 0.385257 | 1.3:-75568989 | MS.gene71833:CDS |
| GCTGATGTTGAGAGCTTATT+GGG | 0.387062 | 1.3:-75569016 | MS.gene71833:CDS |
| CAAAAGTGTCCCTGGACTAA+TGG | 0.395385 | 1.3:-75568668 | MS.gene71833:CDS |
| ATTCCTCGCTGGTTCGCTGC+TGG | 0.398768 | 1.3:+75569357 | None:intergenic |
| TGTCATCCAGTAACGGTAGA+TGG | 0.408098 | 1.3:-75568162 | MS.gene71833:CDS |
| CTCTCATTGTATTTAACATC+AGG | 0.418189 | 1.3:+75569133 | None:intergenic |
| GGAGATAATATGATGGAGAT+TGG | 0.419606 | 1.3:-75568860 | MS.gene71833:CDS |
| GAAGGCAAGAACACCAAGAC+AGG | 0.424009 | 1.3:-75569404 | MS.gene71833:CDS |
| GCAGAGAGGCAAGGGTTTCA+AGG | 0.431755 | 1.3:-75568137 | MS.gene71833:CDS |
| TGTATAGGGATGGAAGAGTT+GGG | 0.440723 | 1.3:-75568974 | MS.gene71833:CDS |
| ATAAATTATACCATGCAATC+AGG | 0.445629 | 1.3:+75568611 | None:intergenic |
| GATTTGTGTTTCCCCTGTCT+TGG | 0.455579 | 1.3:+75569391 | None:intergenic |
| TGAAAGGGAGATTTGTGAAG+AGG | 0.460030 | 1.3:-75568029 | MS.gene71833:CDS |
| ATTTGGATGCTGATGAGAAC+TGG | 0.465263 | 1.3:-75568634 | MS.gene71833:CDS |
| GTGTATAGGGATGGAAGAGT+TGG | 0.474392 | 1.3:-75568975 | MS.gene71833:CDS |
| TGGCTTGTCACCATTAGTCC+AGG | 0.479305 | 1.3:+75568658 | None:intergenic |
| AAATCCGGCACAAGATGAAA+CGG | 0.483022 | 1.3:+75569322 | None:intergenic |
| AGTGAGAAGTGGAAGTGTAG+TGG | 0.486251 | 1.3:-75568926 | MS.gene71833:CDS |
| CCTTCAGTTGGTTCAACTGA+AGG | 0.490709 | 1.3:-75569193 | MS.gene71833:CDS |
| GTTGAGAGCTTATTGGGTAG+GGG | 0.493508 | 1.3:-75569010 | MS.gene71833:CDS |
| TGTACTAGTCCTCCTTCAGT+TGG | 0.493939 | 1.3:-75569205 | MS.gene71833:CDS |
| CTGTTGGAGGCAAAACTGCT+AGG | 0.494037 | 1.3:-75569630 | MS.gene71833:CDS |
| AGTGGAAAGGTGAAGGAAGA+AGG | 0.494618 | 1.3:-75568908 | MS.gene71833:CDS |
| TGTTGAGAGCTTATTGGGTA+GGG | 0.498854 | 1.3:-75569011 | MS.gene71833:CDS |
| TATGCTTATGGTGAGTTTGG+TGG | 0.509644 | 1.3:-75568192 | MS.gene71833:CDS |
| AGAGAGCTATGAAATTGTTG+AGG | 0.512413 | 1.3:-75568882 | MS.gene71833:CDS |
| AGATGGAGGCAGAGAGGCAA+GGG | 0.513332 | 1.3:-75568145 | MS.gene71833:CDS |
| GAAAGGGAGATTTGTGAAGA+GGG | 0.514753 | 1.3:-75568028 | MS.gene71833:CDS |
| GAGAAGAGACCAAGAATGAA+AGG | 0.514881 | 1.3:-75568045 | MS.gene71833:CDS |
| ATATTATTATTATTCCTCGC+TGG | 0.519712 | 1.3:+75569346 | None:intergenic |
| GAGAGCTATGAAATTGTTGA+GGG | 0.520749 | 1.3:-75568881 | MS.gene71833:CDS |
| GGGATAGCCAAAAGTGTCCC+TGG | 0.521182 | 1.3:-75568676 | MS.gene71833:CDS |
| AATCCGGCACAAGATGAAAC+GGG | 0.522679 | 1.3:+75569323 | None:intergenic |
| ATGTTGAGAGCTTATTGGGT+AGG | 0.523810 | 1.3:-75569012 | MS.gene71833:CDS |
| GGTTCAACTGAAGGAGGACT+AGG | 0.524616 | 1.3:-75569184 | MS.gene71833:CDS |
| ACTTCATCGAAACCTAAATC+CGG | 0.535576 | 1.3:+75569307 | None:intergenic |
| TGAAACACTTGAAGAGGTGA+AGG | 0.552820 | 1.3:-75568792 | MS.gene71833:CDS |
| AACGTAGCAAACGCTGTTGG+AGG | 0.555203 | 1.3:-75569643 | MS.gene71833:CDS |
| GAAGAGGTGAAGGAGAAGTG+TGG | 0.556028 | 1.3:-75568782 | MS.gene71833:CDS |
| CCTCGCTGGTTCGCTGCTGG+AGG | 0.557584 | 1.3:+75569360 | None:intergenic |
| TTCTCATGAAACACTTGAAG+AGG | 0.559028 | 1.3:-75568798 | MS.gene71833:CDS |
| ATGATGCAGTAATCATTGCA+TGG | 0.567325 | 1.3:-75568697 | MS.gene71833:CDS |
| TCGGCTAATTCAATATCAGA+TGG | 0.569236 | 1.3:+75569046 | None:intergenic |
| AGAAGAGACCAAGAATGAAA+GGG | 0.570209 | 1.3:-75568044 | MS.gene71833:CDS |
| GAAGTGTAGTGGAAAGGTGA+AGG | 0.573136 | 1.3:-75568915 | MS.gene71833:CDS |
| TGATGCAGTAATCATTGCAT+GGG | 0.576146 | 1.3:-75568696 | MS.gene71833:CDS |
| AGCATGAGGAGAGTGAGAAG+TGG | 0.578354 | 1.3:-75568937 | MS.gene71833:CDS |
| TAGATGGAGGCAGAGAGGCA+AGG | 0.578587 | 1.3:-75568146 | MS.gene71833:CDS |
| GGCTTGTCACCATTAGTCCA+GGG | 0.579867 | 1.3:+75568659 | None:intergenic |
| TGTTGAGGGAGATAATATGA+TGG | 0.580179 | 1.3:-75568867 | MS.gene71833:CDS |
| AAAATAATGATTATAATAAG+GGG | 0.580910 | 1.3:-75568748 | MS.gene71833:CDS |
| TGTTGGAGGCAAAACTGCTA+GGG | 0.581207 | 1.3:-75569629 | MS.gene71833:CDS |
| TATCCCGTTTCATCTTGTGC+CGG | 0.584262 | 1.3:-75569326 | MS.gene71833:CDS |
| TCAGTTGGTTCAACTGAAGG+AGG | 0.585447 | 1.3:-75569190 | MS.gene71833:CDS |
| AGAAAATGAGTGTATAGGGA+TGG | 0.586862 | 1.3:-75568984 | MS.gene71833:CDS |
| GGTGATGAATTCTTCAATTG+TGG | 0.591451 | 1.3:-75569448 | MS.gene71833:CDS |
| AGGAAATGTTGGAACTGCAA+AGG | 0.601959 | 1.3:+75568002 | None:intergenic |
| CCTTCAGTTGAACCAACTGA+AGG | 0.603328 | 1.3:+75569193 | None:intergenic |
| TGAGAACTGGCCTGATTGCA+TGG | 0.605682 | 1.3:-75568621 | MS.gene71833:intron |
| TCAGTTGAACCAACTGAAGG+AGG | 0.611596 | 1.3:+75569196 | None:intergenic |
| ATTGATTGATACAAAGCATG+AGG | 0.625826 | 1.3:-75568951 | MS.gene71833:CDS |
| TAGTGATAATAATTATGAGA+TGG | 0.628845 | 1.3:-75569089 | MS.gene71833:CDS |
| TGAAGCGCAACTTCTTTACA+GGG | 0.630911 | 1.3:-75569260 | MS.gene71833:CDS |
| GTACATAATTCAGCAACAGA+AGG | 0.631423 | 1.3:+75569223 | None:intergenic |
| CTCAAAGAAAATAAGGTACG+AGG | 0.632554 | 1.3:-75568085 | MS.gene71833:CDS |
| AACGGTAGATGGAGGCAGAG+AGG | 0.643178 | 1.3:-75568151 | MS.gene71833:CDS |
| ACAGAGAAAAGCGAAGAACA+AGG | 0.643427 | 1.3:-75568113 | MS.gene71833:CDS |
| AGGCAAGAACACCAAGACAG+GGG | 0.648532 | 1.3:-75569402 | MS.gene71833:CDS |
| GAAGTGGAAGTGTAGTGGAA+AGG | 0.652932 | 1.3:-75568921 | MS.gene71833:CDS |
| AAGGCAAGAACACCAAGACA+GGG | 0.653824 | 1.3:-75569403 | MS.gene71833:CDS |
| ATATGGATGTCATCCAGTAA+CGG | 0.662808 | 1.3:-75568169 | MS.gene71833:CDS |
| AACTGAAGGAGGACTAGGAG+CGG | 0.667542 | 1.3:-75569179 | MS.gene71833:CDS |
| CATCCAGTAACGGTAGATGG+AGG | 0.676181 | 1.3:-75568159 | MS.gene71833:CDS |
| CCTCCAGCAGCGAACCAGCG+AGG | 0.699815 | 1.3:-75569360 | MS.gene71833:CDS |
| GCATAACAAAGAGAGCACGT+TGG | 0.777160 | 1.3:-75569594 | MS.gene71833:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAATGATTATAATAAG+GGG | - | chr1.3:75568903-75568922 | MS.gene71833:CDS | 10.0% |
| !! | AAAATATCTAAATGTATATT+TGG | + | chr1.3:75569190-75569209 | None:intergenic | 10.0% |
| !! | GAAAATAATGATTATAATAA+GGG | - | chr1.3:75568902-75568921 | MS.gene71833:CDS | 10.0% |
| !! | TGAAAATAATGATTATAATA+AGG | - | chr1.3:75568901-75568920 | MS.gene71833:CDS | 10.0% |
| !!! | CTTTCTATCAAATTTTATAA+AGG | - | chr1.3:75569360-75569379 | MS.gene71833:CDS | 15.0% |
| !!! | TATATCTTAATTTTTCTTCA+TGG | - | chr1.3:75569060-75569079 | MS.gene71833:CDS | 15.0% |
| !! | ATTTGATAGAAAGAAAAAAG+TGG | + | chr1.3:75569353-75569372 | None:intergenic | 20.0% |
| !! | TAGTGATAATAATTATGAGA+TGG | - | chr1.3:75568562-75568581 | MS.gene71833:intron | 20.0% |
| !! | TTTACTGTTAGATTAATCAT+TGG | - | chr1.3:75569327-75569346 | MS.gene71833:CDS | 20.0% |
| ! | ATAAATTATACCATGCAATC+AGG | + | chr1.3:75569043-75569062 | None:intergenic | 25.0% |
| ! | ATATTATTATTATTCCTCGC+TGG | + | chr1.3:75568308-75568327 | None:intergenic | 25.0% |
| ! | GATACCAATCATTAACTAAA+GGG | + | chr1.3:75569111-75569130 | None:intergenic | 25.0% |
| ! | GATTAGAAAATGAGTGTATA+GGG | - | chr1.3:75568663-75568682 | MS.gene71833:CDS | 25.0% |
| ! | TGATACCAATCATTAACTAA+AGG | + | chr1.3:75569112-75569131 | None:intergenic | 25.0% |
| !! | ATTATTTATCAGGGAACTTT+TGG | - | chr1.3:75569423-75569442 | MS.gene71833:CDS | 25.0% |
| !!! | TATTTTAGCAAAGGAAATGT+TGG | + | chr1.3:75569663-75569682 | None:intergenic | 25.0% |
| ATTGATTGATACAAAGCATG+AGG | - | chr1.3:75568700-75568719 | MS.gene71833:CDS | 30.0% | |
| CAATCCCTTTAGTTAATGAT+TGG | - | chr1.3:75569104-75569123 | MS.gene71833:CDS | 30.0% | |
| CTCTCATTGTATTTAACATC+AGG | + | chr1.3:75568521-75568540 | None:intergenic | 30.0% | |
| GAAGAATTCATCACCATTAA+TGG | + | chr1.3:75568198-75568217 | None:intergenic | 30.0% | |
| GGATTAGAAAATGAGTGTAT+AGG | - | chr1.3:75568662-75568681 | MS.gene71833:CDS | 30.0% | |
| GGTTGTTCTCAAAGAAAATA+AGG | - | chr1.3:75569559-75569578 | MS.gene71833:CDS | 30.0% | |
| ACAAATCTCCCTTTCATTCT+TGG | + | chr1.3:75569618-75569637 | None:intergenic | 35.0% | |
| ACAGTAAAAAAGTGAACAGC+AGG | + | chr1.3:75569315-75569334 | None:intergenic | 35.0% | |
| ACTTCATCGAAACCTAAATC+CGG | + | chr1.3:75568347-75568366 | None:intergenic | 35.0% | |
| AGAAAATAAGGTACGAGGTT+AGG | - | chr1.3:75569571-75569590 | MS.gene71833:CDS | 35.0% | |
| AGAAAATGAGTGTATAGGGA+TGG | - | chr1.3:75568667-75568686 | MS.gene71833:CDS | 35.0% | |
| AGAAGAGACCAAGAATGAAA+GGG | - | chr1.3:75569607-75569626 | MS.gene71833:CDS | 35.0% | |
| AGAGAGCTATGAAATTGTTG+AGG | - | chr1.3:75568769-75568788 | MS.gene71833:CDS | 35.0% | |
| ATATGGATGTCATCCAGTAA+CGG | - | chr1.3:75569482-75569501 | MS.gene71833:CDS | 35.0% | |
| ATGATGCAGTAATCATTGCA+TGG | - | chr1.3:75568954-75568973 | MS.gene71833:CDS | 35.0% | |
| CATGAGTGTGTCAAAAATCA+CGG | + | chr1.3:75569161-75569180 | None:intergenic | 35.0% | |
| CATTATGCTTATGGTGAGTT+TGG | - | chr1.3:75569456-75569475 | MS.gene71833:CDS | 35.0% | |
| CTCAAAGAAAATAAGGTACG+AGG | - | chr1.3:75569566-75569585 | MS.gene71833:CDS | 35.0% | |
| GAGAGCTATGAAATTGTTGA+GGG | - | chr1.3:75568770-75568789 | MS.gene71833:CDS | 35.0% | |
| GGTGATGAATTCTTCAATTG+TGG | - | chr1.3:75568203-75568222 | MS.gene71833:CDS | 35.0% | |
| GTACATAATTCAGCAACAGA+AGG | + | chr1.3:75568431-75568450 | None:intergenic | 35.0% | |
| TAATGGTGACAAGCCAAATT+TGG | - | chr1.3:75569000-75569019 | MS.gene71833:CDS | 35.0% | |
| TCGGCTAATTCAATATCAGA+TGG | + | chr1.3:75568608-75568627 | None:intergenic | 35.0% | |
| TGATGCAGTAATCATTGCAT+GGG | - | chr1.3:75568955-75568974 | MS.gene71833:CDS | 35.0% | |
| TGTTGAGGGAGATAATATGA+TGG | - | chr1.3:75568784-75568803 | MS.gene71833:CDS | 35.0% | |
| TTCTCATCAGCATCCAAATT+TGG | + | chr1.3:75569016-75569035 | None:intergenic | 35.0% | |
| TTCTCATGAAACACTTGAAG+AGG | - | chr1.3:75568853-75568872 | MS.gene71833:CDS | 35.0% | |
| ! | GGAGATAATATGATGGAGAT+TGG | - | chr1.3:75568791-75568810 | MS.gene71833:CDS | 35.0% |
| ! | TTCAATTGTGGTCCTTTTTC+TGG | - | chr1.3:75568215-75568234 | MS.gene71833:CDS | 35.0% |
| !! | TTATCAGGGAACTTTTGGAA+CGG | - | chr1.3:75569428-75569447 | MS.gene71833:CDS | 35.0% |
| AAAAACGTAGCAAACGCTGT+TGG | - | chr1.3:75568005-75568024 | MS.gene71833:CDS | 40.0% | |
| AAATCCGGCACAAGATGAAA+CGG | + | chr1.3:75568332-75568351 | None:intergenic | 40.0% | |
| ACAGAGAAAAGCGAAGAACA+AGG | - | chr1.3:75569538-75569557 | MS.gene71833:CDS | 40.0% | |
| ACGGAGGTTCATTATGCTTA+TGG | - | chr1.3:75569447-75569466 | MS.gene71833:CDS | 40.0% | |
| AGCTGATGTTGAGAGCTTAT+TGG | - | chr1.3:75568634-75568653 | MS.gene71833:CDS | 40.0% | |
| AGGAAATGTTGGAACTGCAA+AGG | + | chr1.3:75569652-75569671 | None:intergenic | 40.0% | |
| ATGAAGCGCAACTTCTTTAC+AGG | - | chr1.3:75568390-75568409 | MS.gene71833:intron | 40.0% | |
| ATGTTGAGAGCTTATTGGGT+AGG | - | chr1.3:75568639-75568658 | MS.gene71833:CDS | 40.0% | |
| ATTTGGATGCTGATGAGAAC+TGG | - | chr1.3:75569017-75569036 | MS.gene71833:CDS | 40.0% | |
| CTCAACATCAGCTGCAAATT+CGG | + | chr1.3:75568627-75568646 | None:intergenic | 40.0% | |
| CTTTACAACACTTGCACACA+CGG | - | chr1.3:75569266-75569285 | MS.gene71833:CDS | 40.0% | |
| GAAAGGGAGATTTGTGAAGA+GGG | - | chr1.3:75569623-75569642 | MS.gene71833:CDS | 40.0% | |
| GAGAAGAGACCAAGAATGAA+AGG | - | chr1.3:75569606-75569625 | MS.gene71833:CDS | 40.0% | |
| GCAACAGAAGGATCGAAAAT+TGG | + | chr1.3:75568419-75568438 | None:intergenic | 40.0% | |
| GCTGATGTTGAGAGCTTATT+GGG | - | chr1.3:75568635-75568654 | MS.gene71833:CDS | 40.0% | |
| TATGCTTATGGTGAGTTTGG+TGG | - | chr1.3:75569459-75569478 | MS.gene71833:CDS | 40.0% | |
| TGAAACACTTGAAGAGGTGA+AGG | - | chr1.3:75568859-75568878 | MS.gene71833:CDS | 40.0% | |
| TGAAAGGGAGATTTGTGAAG+AGG | - | chr1.3:75569622-75569641 | MS.gene71833:CDS | 40.0% | |
| TGAAGCGCAACTTCTTTACA+GGG | - | chr1.3:75568391-75568410 | MS.gene71833:intron | 40.0% | |
| TGTATAGGGATGGAAGAGTT+GGG | - | chr1.3:75568677-75568696 | MS.gene71833:CDS | 40.0% | |
| TGTTGAGAGCTTATTGGGTA+GGG | - | chr1.3:75568640-75568659 | MS.gene71833:CDS | 40.0% | |
| TTCTTGGTGAAGCCAGAAAA+AGG | + | chr1.3:75568230-75568249 | None:intergenic | 40.0% | |
| TTTCATCTTGTGCCGGATTT+AGG | - | chr1.3:75568332-75568351 | MS.gene71833:intron | 40.0% | |
| ! | CGTGATTTTTGACACACTCA+TGG | - | chr1.3:75569159-75569178 | MS.gene71833:CDS | 40.0% |
| ! | TATGGTGAGTTTGGTGGATA+TGG | - | chr1.3:75569465-75569484 | MS.gene71833:CDS | 40.0% |
| !!! | TTTTTCTGGCTTCACCAAGA+AGG | - | chr1.3:75568229-75568248 | MS.gene71833:intron | 40.0% |
| AAGGCAAGAACACCAAGACA+GGG | - | chr1.3:75568248-75568267 | MS.gene71833:intron | 45.0% | |
| AATCCGGCACAAGATGAAAC+GGG | + | chr1.3:75568331-75568350 | None:intergenic | 45.0% | |
| AGTGGAAAGGTGAAGGAAGA+AGG | - | chr1.3:75568743-75568762 | MS.gene71833:CDS | 45.0% | |
| CCTTCAGTTGAACCAACTGA+AGG | + | chr1.3:75568461-75568480 | None:intergenic | 45.0% | |
| GATTTGTGTTTCCCCTGTCT+TGG | + | chr1.3:75568263-75568282 | None:intergenic | 45.0% | |
| GCAGGTCTAACACAAGTGAA+TGG | + | chr1.3:75569297-75569316 | None:intergenic | 45.0% | |
| GCGTCGTACAAGTCCATTAA+TGG | - | chr1.3:75568182-75568201 | MS.gene71833:CDS | 45.0% | |
| GTGTATAGGGATGGAAGAGT+TGG | - | chr1.3:75568676-75568695 | MS.gene71833:CDS | 45.0% | |
| GTTGAGAGCTTATTGGGTAG+GGG | - | chr1.3:75568641-75568660 | MS.gene71833:CDS | 45.0% | |
| TATCCCGTTTCATCTTGTGC+CGG | - | chr1.3:75568325-75568344 | MS.gene71833:intron | 45.0% | |
| TCAGTTGAACCAACTGAAGG+AGG | + | chr1.3:75568458-75568477 | None:intergenic | 45.0% | |
| TGTACTAGTCCTCCTTCAGT+TGG | - | chr1.3:75568446-75568465 | MS.gene71833:intron | 45.0% | |
| TGTCATCCAGTAACGGTAGA+TGG | - | chr1.3:75569489-75569508 | MS.gene71833:CDS | 45.0% | |
| TGTTGGAGGCAAAACTGCTA+GGG | - | chr1.3:75568022-75568041 | MS.gene71833:CDS | 45.0% | |
| ! | AGTGAGAAGTGGAAGTGTAG+TGG | - | chr1.3:75568725-75568744 | MS.gene71833:CDS | 45.0% |
| ! | CATTAGTCCAGGGACACTTT+TGG | + | chr1.3:75568985-75569004 | None:intergenic | 45.0% |
| ! | CCTTCAGTTGGTTCAACTGA+AGG | - | chr1.3:75568458-75568477 | MS.gene71833:intron | 45.0% |
| ! | GAAGTGGAAGTGTAGTGGAA+AGG | - | chr1.3:75568730-75568749 | MS.gene71833:CDS | 45.0% |
| ! | GAAGTGTAGTGGAAAGGTGA+AGG | - | chr1.3:75568736-75568755 | MS.gene71833:CDS | 45.0% |
| ! | GCATAACAAAGAGAGCACGT+TGG | - | chr1.3:75568057-75568076 | MS.gene71833:CDS | 45.0% |
| ! | TCAGTTGGTTCAACTGAAGG+AGG | - | chr1.3:75568461-75568480 | MS.gene71833:intron | 45.0% |
| !! | CAAAAGTGTCCCTGGACTAA+TGG | - | chr1.3:75568983-75569002 | MS.gene71833:CDS | 45.0% |
| !! | TCTTGGTGTTCTTGCCTTCT+TGG | + | chr1.3:75568246-75568265 | None:intergenic | 45.0% |
| !!! | TTTAATTTAATTATTTATCA+GGG | - | chr1.3:75569414-75569433 | MS.gene71833:CDS | 5.0% |
| !!! | TTTTAATTTAATTATTTATC+AGG | - | chr1.3:75569413-75569432 | MS.gene71833:CDS | 5.0% |
| AACGTAGCAAACGCTGTTGG+AGG | - | chr1.3:75568008-75568027 | MS.gene71833:CDS | 50.0% | |
| AACTGAAGGAGGACTAGGAG+CGG | - | chr1.3:75568472-75568491 | MS.gene71833:intron | 50.0% | |
| AGCATGAGGAGAGTGAGAAG+TGG | - | chr1.3:75568714-75568733 | MS.gene71833:CDS | 50.0% | |
| AGGCAAGAACACCAAGACAG+GGG | - | chr1.3:75568249-75568268 | MS.gene71833:intron | 50.0% | |
| CATCCAGTAACGGTAGATGG+AGG | - | chr1.3:75569492-75569511 | MS.gene71833:CDS | 50.0% | |
| CTGTTGGAGGCAAAACTGCT+AGG | - | chr1.3:75568021-75568040 | MS.gene71833:CDS | 50.0% | |
| GAAGAGGTGAAGGAGAAGTG+TGG | - | chr1.3:75568869-75568888 | MS.gene71833:CDS | 50.0% | |
| GAAGGCAAGAACACCAAGAC+AGG | - | chr1.3:75568247-75568266 | MS.gene71833:intron | 50.0% | |
| GGCTTGTCACCATTAGTCCA+GGG | + | chr1.3:75568995-75569014 | None:intergenic | 50.0% | |
| GGTTCAACTGAAGGAGGACT+AGG | - | chr1.3:75568467-75568486 | MS.gene71833:intron | 50.0% | |
| ! | TGAGAACTGGCCTGATTGCA+TGG | - | chr1.3:75569030-75569049 | MS.gene71833:CDS | 50.0% |
| ! | TGGCTTGTCACCATTAGTCC+AGG | + | chr1.3:75568996-75569015 | None:intergenic | 50.0% |
| !!! | TCAGGGAACTTTTGGAACGG+AGG | - | chr1.3:75569431-75569450 | MS.gene71833:CDS | 50.0% |
| AACGGTAGATGGAGGCAGAG+AGG | - | chr1.3:75569500-75569519 | MS.gene71833:CDS | 55.0% | |
| AGATGGAGGCAGAGAGGCAA+GGG | - | chr1.3:75569506-75569525 | MS.gene71833:CDS | 55.0% | |
| CTGCCTCCATCTACCGTTAC+TGG | + | chr1.3:75569498-75569517 | None:intergenic | 55.0% | |
| GCAGAGAGGCAAGGGTTTCA+AGG | - | chr1.3:75569514-75569533 | MS.gene71833:CDS | 55.0% | |
| TAGATGGAGGCAGAGAGGCA+AGG | - | chr1.3:75569505-75569524 | MS.gene71833:CDS | 55.0% | |
| !! | GGGATAGCCAAAAGTGTCCC+TGG | - | chr1.3:75568975-75568994 | MS.gene71833:CDS | 55.0% |
| !! | ATTCCTCGCTGGTTCGCTGC+TGG | + | chr1.3:75568297-75568316 | None:intergenic | 60.0% |
| CCTCCAGCAGCGAACCAGCG+AGG | - | chr1.3:75568291-75568310 | MS.gene71833:intron | 70.0% | |
| !! | CCTCGCTGGTTCGCTGCTGG+AGG | + | chr1.3:75568294-75568313 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 75567990 | 75569683 | 75567990 | ID=MS.gene71833 |
| chr1.3 | mRNA | 75567990 | 75569683 | 75567990 | ID=MS.gene71833.t1;Parent=MS.gene71833 |
| chr1.3 | exon | 75568622 | 75569683 | 75568622 | ID=MS.gene71833.t1.exon1;Parent=MS.gene71833.t1 |
| chr1.3 | CDS | 75568622 | 75569683 | 75568622 | ID=cds.MS.gene71833.t1;Parent=MS.gene71833.t1 |
| chr1.3 | exon | 75567990 | 75568238 | 75567990 | ID=MS.gene71833.t1.exon2;Parent=MS.gene71833.t1 |
| chr1.3 | CDS | 75567990 | 75568238 | 75567990 | ID=cds.MS.gene71833.t1;Parent=MS.gene71833.t1 |
| Gene Sequence |
| Protein sequence |
