Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94277.t1 | XP_013452267.1 | 94.5 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.80E-90 | 341.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94277.t1 | Q9LTD9 | 43.3 | 134 | 68 | 1 | 45 | 178 | 42 | 167 | 2.0e-23 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94277.t1 | A0A072UKD7 | 94.5 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.0e-90 | 341.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene94277 | 0.843292 | 1.53E-58 | -1.69E-46 |
| MS.gene049577 | MS.gene94277 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene94277 | 0.829157 | 6.02E-55 | -1.69E-46 |
| MS.gene049662 | MS.gene94277 | 0.830346 | 3.09E-55 | -1.69E-46 |
| MS.gene049758 | MS.gene94277 | 0.843034 | 1.80E-58 | -1.69E-46 |
| MS.gene049910 | MS.gene94277 | 0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene94277 | 0.856357 | 3.40E-62 | -1.69E-46 |
| MS.gene050301 | MS.gene94277 | 0.830855 | 2.32E-55 | -1.69E-46 |
| MS.gene050397 | MS.gene94277 | 0.869741 | 2.47E-66 | -1.69E-46 |
| MS.gene050404 | MS.gene94277 | 0.909818 | 3.74E-82 | -1.69E-46 |
| MS.gene050406 | MS.gene94277 | 0.920236 | 1.65E-87 | -1.69E-46 |
| MS.gene050426 | MS.gene94277 | -0.82849 | 8.73E-55 | -1.69E-46 |
| MS.gene050432 | MS.gene94277 | 0.80199 | 6.94E-49 | -1.69E-46 |
| MS.gene050464 | MS.gene94277 | 0.862084 | 6.51E-64 | -1.69E-46 |
| MS.gene050529 | MS.gene94277 | 0.887544 | 1.30E-72 | -1.69E-46 |
| MS.gene050642 | MS.gene94277 | 0.835004 | 2.16E-56 | -1.69E-46 |
| MS.gene050670 | MS.gene94277 | 0.809768 | 1.61E-50 | -1.69E-46 |
| MS.gene050812 | MS.gene94277 | 0.819694 | 1.02E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene94277 | 0.834331 | 3.19E-56 | -1.69E-46 |
| MS.gene051171 | MS.gene94277 | 0.855664 | 5.42E-62 | -1.69E-46 |
| MS.gene051240 | MS.gene94277 | 0.827347 | 1.64E-54 | -1.69E-46 |
| MS.gene051242 | MS.gene94277 | 0.844161 | 8.97E-59 | -1.69E-46 |
| MS.gene051244 | MS.gene94277 | 0.806329 | 8.67E-50 | -1.69E-46 |
| MS.gene051471 | MS.gene94277 | 0.865865 | 4.33E-65 | -1.69E-46 |
| MS.gene051582 | MS.gene94277 | 0.850646 | 1.48E-60 | -1.69E-46 |
| MS.gene051983 | MS.gene94277 | 0.821751 | 3.42E-53 | -1.69E-46 |
| MS.gene052011 | MS.gene94277 | -0.804669 | 1.93E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene94277 | 0.851362 | 9.32E-61 | -1.69E-46 |
| MS.gene052078 | MS.gene94277 | 0.873452 | 1.45E-67 | -1.69E-46 |
| MS.gene052178 | MS.gene94277 | 0.848573 | 5.63E-60 | -1.69E-46 |
| MS.gene052188 | MS.gene94277 | 0.824254 | 8.92E-54 | -1.69E-46 |
| MS.gene052253 | MS.gene94277 | 0.826146 | 3.18E-54 | -1.69E-46 |
| MS.gene052290 | MS.gene94277 | 0.81981 | 9.55E-53 | -1.69E-46 |
| MS.gene052310 | MS.gene94277 | 0.873865 | 1.06E-67 | -1.69E-46 |
| MS.gene052322 | MS.gene94277 | 0.808966 | 2.39E-50 | -1.69E-46 |
| MS.gene052326 | MS.gene94277 | 0.81185 | 5.70E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene94277 | 0.861135 | 1.27E-63 | -1.69E-46 |
| MS.gene052758 | MS.gene94277 | 0.809937 | 1.48E-50 | -1.69E-46 |
| MS.gene053073 | MS.gene94277 | 0.80898 | 2.37E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene94277 | 0.80829 | 3.33E-50 | -1.69E-46 |
| MS.gene053209 | MS.gene94277 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene053330 | MS.gene94277 | 0.843426 | 1.41E-58 | -1.69E-46 |
| MS.gene053557 | MS.gene94277 | 0.849636 | 2.85E-60 | -1.69E-46 |
| MS.gene053836 | MS.gene94277 | 0.865197 | 7.03E-65 | -1.69E-46 |
| MS.gene053907 | MS.gene94277 | 0.872874 | 2.27E-67 | -1.69E-46 |
| MS.gene053928 | MS.gene94277 | 0.823584 | 1.28E-53 | -1.69E-46 |
| MS.gene053971 | MS.gene94277 | 0.909988 | 3.10E-82 | -1.69E-46 |
| MS.gene054123 | MS.gene94277 | 0.811224 | 7.79E-51 | -1.69E-46 |
| MS.gene05413 | MS.gene94277 | 0.800162 | 1.64E-48 | -1.69E-46 |
| MS.gene054349 | MS.gene94277 | 0.826158 | 3.16E-54 | -1.69E-46 |
| MS.gene054918 | MS.gene94277 | 0.807599 | 4.67E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene94277 | 0.84667 | 1.88E-59 | -1.69E-46 |
| MS.gene055116 | MS.gene94277 | 0.848214 | 7.07E-60 | -1.69E-46 |
| MS.gene055254 | MS.gene94277 | 0.807378 | 5.20E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene94277 | 0.859617 | 3.65E-63 | -1.69E-46 |
| MS.gene055370 | MS.gene94277 | 0.838584 | 2.64E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene94277 | 0.840107 | 1.06E-57 | -1.69E-46 |
| MS.gene055428 | MS.gene94277 | 0.874454 | 6.67E-68 | -1.69E-46 |
| MS.gene05553 | MS.gene94277 | 0.851048 | 1.14E-60 | -1.69E-46 |
| MS.gene055601 | MS.gene94277 | -0.804435 | 2.16E-49 | -1.69E-46 |
| MS.gene055712 | MS.gene94277 | 0.814942 | 1.19E-51 | -1.69E-46 |
| MS.gene055980 | MS.gene94277 | 0.827433 | 1.57E-54 | -1.69E-46 |
| MS.gene056191 | MS.gene94277 | 0.801154 | 1.03E-48 | -1.69E-46 |
| MS.gene056407 | MS.gene94277 | 0.836746 | 7.81E-57 | -1.69E-46 |
| MS.gene056451 | MS.gene94277 | 0.870423 | 1.48E-66 | -1.69E-46 |
| MS.gene056612 | MS.gene94277 | 0.885035 | 1.15E-71 | -1.69E-46 |
| MS.gene056633 | MS.gene94277 | 0.852295 | 5.07E-61 | -1.69E-46 |
| MS.gene056659 | MS.gene94277 | 0.825853 | 3.74E-54 | -1.69E-46 |
| MS.gene056664 | MS.gene94277 | 0.867907 | 9.68E-66 | -1.69E-46 |
| MS.gene056796 | MS.gene94277 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene056899 | MS.gene94277 | -0.844614 | 6.78E-59 | -1.69E-46 |
| MS.gene056979 | MS.gene94277 | 0.813615 | 2.34E-51 | -1.69E-46 |
| MS.gene05702 | MS.gene94277 | 0.898563 | 4.70E-77 | -1.69E-46 |
| MS.gene057121 | MS.gene94277 | 0.809989 | 1.44E-50 | -1.69E-46 |
| MS.gene057123 | MS.gene94277 | 0.809748 | 1.62E-50 | -1.69E-46 |
| MS.gene057245 | MS.gene94277 | 0.804595 | 2.00E-49 | -1.69E-46 |
| MS.gene057295 | MS.gene94277 | 0.801649 | 8.15E-49 | -1.69E-46 |
| MS.gene05733 | MS.gene94277 | 0.817082 | 3.97E-52 | -1.69E-46 |
| MS.gene057439 | MS.gene94277 | 0.826527 | 2.58E-54 | -1.69E-46 |
| MS.gene058002 | MS.gene94277 | 0.857714 | 1.35E-62 | -1.69E-46 |
| MS.gene058082 | MS.gene94277 | 0.826348 | 2.85E-54 | -1.69E-46 |
| MS.gene05811 | MS.gene94277 | 0.824192 | 9.22E-54 | -1.69E-46 |
| MS.gene058142 | MS.gene94277 | 0.856694 | 2.70E-62 | -1.69E-46 |
| MS.gene058233 | MS.gene94277 | 0.899554 | 1.77E-77 | -1.69E-46 |
| MS.gene058276 | MS.gene94277 | 0.880947 | 3.61E-70 | -1.69E-46 |
| MS.gene058293 | MS.gene94277 | 0.814186 | 1.75E-51 | -1.69E-46 |
| MS.gene058365 | MS.gene94277 | 0.813436 | 2.56E-51 | -1.69E-46 |
| MS.gene058660 | MS.gene94277 | 0.827595 | 1.43E-54 | -1.69E-46 |
| MS.gene058710 | MS.gene94277 | 0.821289 | 4.37E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene94277 | 0.886526 | 3.17E-72 | -1.69E-46 |
| MS.gene058749 | MS.gene94277 | 0.808555 | 2.92E-50 | -1.69E-46 |
| MS.gene058845 | MS.gene94277 | 0.813588 | 2.37E-51 | -1.69E-46 |
| MS.gene059011 | MS.gene94277 | 0.809706 | 1.66E-50 | -1.69E-46 |
| MS.gene05907 | MS.gene94277 | 0.8435 | 1.35E-58 | -1.69E-46 |
| MS.gene059285 | MS.gene94277 | 0.827831 | 1.26E-54 | -1.69E-46 |
| MS.gene059518 | MS.gene94277 | 0.831904 | 1.28E-55 | -1.69E-46 |
| MS.gene059597 | MS.gene94277 | 0.841715 | 4.02E-58 | -1.69E-46 |
| MS.gene059676 | MS.gene94277 | 0.801015 | 1.10E-48 | -1.69E-46 |
| MS.gene059688 | MS.gene94277 | 0.8213 | 4.35E-53 | -1.69E-46 |
| MS.gene059692 | MS.gene94277 | 0.84308 | 1.75E-58 | -1.69E-46 |
| MS.gene059695 | MS.gene94277 | 0.841012 | 6.15E-58 | -1.69E-46 |
| MS.gene05984 | MS.gene94277 | 0.834882 | 2.32E-56 | -1.69E-46 |
| MS.gene059894 | MS.gene94277 | 0.907022 | 7.94E-81 | -1.69E-46 |
| MS.gene059899 | MS.gene94277 | 0.801409 | 9.13E-49 | -1.69E-46 |
| MS.gene06037 | MS.gene94277 | 0.829125 | 6.13E-55 | -1.69E-46 |
| MS.gene060403 | MS.gene94277 | 0.806555 | 7.77E-50 | -1.69E-46 |
| MS.gene060407 | MS.gene94277 | 0.87391 | 1.02E-67 | -1.69E-46 |
| MS.gene06090 | MS.gene94277 | 0.814519 | 1.48E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene94277 | 0.910939 | 1.07E-82 | -1.69E-46 |
| MS.gene061112 | MS.gene94277 | 0.849753 | 2.64E-60 | -1.69E-46 |
| MS.gene061159 | MS.gene94277 | 0.812046 | 5.16E-51 | -1.69E-46 |
| MS.gene061265 | MS.gene94277 | 0.845264 | 4.53E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94277.t1 | MTR_6g051780 | 94.536 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.10e-112 | 318 |
| MS.gene94277.t1 | MTR_5g027010 | 34.356 | 163 | 90 | 3 | 29 | 176 | 33 | 193 | 6.26e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene94277.t1 | AT5G52780 | 50.000 | 94 | 47 | 0 | 85 | 178 | 74 | 167 | 1.72e-30 | 109 |
| MS.gene94277.t1 | AT4G19100 | 43.564 | 101 | 57 | 0 | 83 | 183 | 49 | 149 | 3.49e-22 | 87.8 |
| MS.gene94277.t1 | AT4G19100 | 43.564 | 101 | 57 | 0 | 83 | 183 | 112 | 212 | 4.53e-22 | 89.4 |
Find 0 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGTTATGTATAGAATAAT+CGG | - | 43125:10359-10378 | MS.gene94277:CDS | 20.0% |
| !!! | TTGAATTGTTTTGATGTATT+GGG | + | 43125:10197-10216 | MS.gene94277:intergenic | 20.0% |
| ! | ATCATCATCATTCTTATTGT+TGG | + | 43125:10327-10346 | MS.gene94277:intergenic | 25.0% |
| ! | ATTCTATACATAACTTCCTT+AGG | + | 43125:10356-10375 | MS.gene94277:intergenic | 25.0% |
| ! | TCATCATCATTCTTATTGTT+GGG | + | 43125:10326-10345 | MS.gene94277:intergenic | 25.0% |
| !!! | ATGTATTGGGTTGAAAAAAT+GGG | + | 43125:10184-10203 | MS.gene94277:intergenic | 25.0% |
| !!! | GTTGAATTGTTTTGATGTAT+TGG | + | 43125:10198-10217 | MS.gene94277:intergenic | 25.0% |
| AAATATTTGATGCTCCTTTG+TGG | - | 43125:10462-10481 | MS.gene94277:CDS | 30.0% | |
| AATACAAGAGTTGTCACAAA+TGG | + | 43125:10491-10510 | MS.gene94277:intergenic | 30.0% | |
| ACCATCAACAAATAAAGCTA+AGG | - | 43125:10277-10296 | MS.gene94277:CDS | 30.0% | |
| TGAAGAAGTTGAGAAGAATT+GGG | - | 43125:10595-10614 | MS.gene94277:CDS | 30.0% | |
| TTATCATTCCTGCATCTTTA+TGG | - | 43125:10422-10441 | MS.gene94277:CDS | 30.0% | |
| TTGAAGAAGTTGAGAAGAAT+TGG | - | 43125:10594-10613 | MS.gene94277:CDS | 30.0% | |
| ! | GTTTAGTACTTTAGTTCCAA+TGG | - | 43125:10391-10410 | MS.gene94277:CDS | 30.0% |
| ! | TGAAAAAATGGGAAGTTTTG+TGG | + | 43125:10173-10192 | MS.gene94277:intergenic | 30.0% |
| ! | TGATGATGATGATATTCCTA+AGG | - | 43125:10337-10356 | MS.gene94277:CDS | 30.0% |
| !! | AAGTTTTGTGGTTTACGTAT+TGG | + | 43125:10161-10180 | MS.gene94277:intergenic | 30.0% |
| !! | GATGTATTGGGTTGAAAAAA+TGG | + | 43125:10185-10204 | MS.gene94277:intergenic | 30.0% |
| !! | TCCTTAGCTTTATTTGTTGA+TGG | + | 43125:10281-10300 | MS.gene94277:intergenic | 30.0% |
| !! | TTAGCTTTATTTGTTGATGG+TGG | + | 43125:10278-10297 | MS.gene94277:intergenic | 30.0% |
| !!! | GTGACAACTCTTGTATTTTT+TGG | - | 43125:10494-10513 | MS.gene94277:CDS | 30.0% |
| AATGATAACCCTAATGCCAT+TGG | + | 43125:10410-10429 | MS.gene94277:intergenic | 35.0% | |
| TTAACTCACCATAAAGATGC+AGG | + | 43125:10433-10452 | MS.gene94277:intergenic | 35.0% | |
| ! | ACTTTAGTTCCAATGGCATT+AGG | - | 43125:10398-10417 | MS.gene94277:CDS | 35.0% |
| ! | CTTTAGTTCCAATGGCATTA+GGG | - | 43125:10399-10418 | MS.gene94277:CDS | 35.0% |
| !!! | CTTGTATTTTTTGGAGCTTC+TGG | - | 43125:10503-10522 | MS.gene94277:CDS | 35.0% |
| AGAGAGAGAAGGTTCTTTGT+TGG | - | 43125:10568-10587 | MS.gene94277:CDS | 40.0% | |
| GAGAGAGAAGGTTCTTTGTT+GGG | - | 43125:10569-10588 | MS.gene94277:CDS | 40.0% | |
| GTGTTGAGCTATGGAGAATT+GGG | + | 43125:10133-10152 | MS.gene94277:intergenic | 40.0% | |
| TCTGGACTTGGAATTGCATA+TGG | - | 43125:10521-10540 | MS.gene94277:CDS | 40.0% | |
| TGTGTTGAGCTATGGAGAAT+TGG | + | 43125:10134-10153 | MS.gene94277:intergenic | 40.0% | |
| TTGGAGATTGTGTTGAGCTA+TGG | + | 43125:10142-10161 | MS.gene94277:intergenic | 40.0% | |
| ! | TGGTGTGCTTTCTTCTAGTT+TGG | - | 43125:10541-10560 | MS.gene94277:CDS | 40.0% |
| !! | AGAAGAATTGGGTTGAGATG+TGG | - | 43125:10606-10625 | MS.gene94277:CDS | 40.0% |
| !!! | TTTTTTGGAGCTTCTGGACT+TGG | - | 43125:10509-10528 | MS.gene94277:CDS | 40.0% |
| AGTTTGGATGCAGAGAGAGA+AGG | - | 43125:10557-10576 | MS.gene94277:CDS | 45.0% | |
| GTCACAAATGGCATCCACAA+AGG | + | 43125:10479-10498 | MS.gene94277:intergenic | 45.0% | |
| TCTTGTGTTGGAGAAGCCTT+TGG | + | 43125:10255-10274 | MS.gene94277:intergenic | 45.0% | |
| !! | TGTTGATGGTGGTCTTGTGT+TGG | + | 43125:10267-10286 | MS.gene94277:intergenic | 45.0% |
| AAGCCACAAGCTAGTGCCAA+AGG | - | 43125:10236-10255 | MS.gene94277:CDS | 50.0% | |
| GTGGCTTCAACGTGGTGAAT+TGG | + | 43125:10223-10242 | MS.gene94277:intergenic | 50.0% | |
| ! | AAGCCTTTGGCACTAGCTTG+TGG | + | 43125:10242-10261 | MS.gene94277:intergenic | 50.0% |
| ! | ACTAGCTTGTGGCTTCAACG+TGG | + | 43125:10231-10250 | MS.gene94277:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43125 | gene | 10104 | 10655 | 10104 | ID=MS.gene94277 |
| 43125 | mRNA | 10104 | 10655 | 10104 | ID=MS.gene94277.t1;Parent=MS.gene94277 |
| 43125 | exon | 10104 | 10655 | 10104 | ID=MS.gene94277.t1.exon1;Parent=MS.gene94277.t1 |
| 43125 | CDS | 10104 | 10655 | 10104 | ID=cds.MS.gene94277.t1;Parent=MS.gene94277.t1 |
| Gene Sequence |
| Protein sequence |
