Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96681.t1 | XP_013452267.1 | 94.5 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.80E-90 | 341.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96681.t1 | Q9LTD9 | 43.3 | 134 | 68 | 1 | 45 | 178 | 42 | 167 | 2.0e-23 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96681.t1 | A0A072UKD7 | 94.5 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.0e-90 | 341.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049284 | MS.gene96681 | 0.800243 | 1.58E-48 | -1.69E-46 |
| MS.gene049346 | MS.gene96681 | 0.834217 | 3.41E-56 | -1.69E-46 |
| MS.gene049577 | MS.gene96681 | 0.806846 | 6.75E-50 | -1.69E-46 |
| MS.gene049661 | MS.gene96681 | 0.821587 | 3.73E-53 | -1.69E-46 |
| MS.gene049662 | MS.gene96681 | 0.822222 | 2.66E-53 | -1.69E-46 |
| MS.gene049758 | MS.gene96681 | 0.830254 | 3.26E-55 | -1.69E-46 |
| MS.gene049910 | MS.gene96681 | 0.806444 | 8.20E-50 | -1.69E-46 |
| MS.gene050076 | MS.gene96681 | 0.860578 | 1.87E-63 | -1.69E-46 |
| MS.gene050301 | MS.gene96681 | 0.824066 | 9.87E-54 | -1.69E-46 |
| MS.gene050397 | MS.gene96681 | 0.859725 | 3.39E-63 | -1.69E-46 |
| MS.gene050404 | MS.gene96681 | 0.917141 | 7.62E-86 | -1.69E-46 |
| MS.gene050406 | MS.gene96681 | 0.923028 | 4.55E-89 | -1.69E-46 |
| MS.gene050426 | MS.gene96681 | -0.812774 | 3.58E-51 | -1.69E-46 |
| MS.gene050464 | MS.gene96681 | 0.853625 | 2.11E-61 | -1.69E-46 |
| MS.gene050529 | MS.gene96681 | 0.878306 | 3.12E-69 | -1.69E-46 |
| MS.gene050642 | MS.gene96681 | 0.821083 | 4.87E-53 | -1.69E-46 |
| MS.gene050812 | MS.gene96681 | 0.815485 | 9.02E-52 | -1.69E-46 |
| MS.gene05101 | MS.gene96681 | 0.839135 | 1.90E-57 | -1.69E-46 |
| MS.gene051171 | MS.gene96681 | 0.864919 | 8.59E-65 | -1.69E-46 |
| MS.gene051240 | MS.gene96681 | 0.830913 | 2.25E-55 | -1.69E-46 |
| MS.gene051242 | MS.gene96681 | 0.852636 | 4.05E-61 | -1.69E-46 |
| MS.gene051244 | MS.gene96681 | 0.816429 | 5.56E-52 | -1.69E-46 |
| MS.gene051471 | MS.gene96681 | 0.870083 | 1.91E-66 | -1.69E-46 |
| MS.gene051582 | MS.gene96681 | 0.851622 | 7.87E-61 | -1.69E-46 |
| MS.gene051662 | MS.gene96681 | 0.801589 | 8.39E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene96681 | 0.803777 | 2.97E-49 | -1.69E-46 |
| MS.gene052077 | MS.gene96681 | 0.83987 | 1.22E-57 | -1.69E-46 |
| MS.gene052078 | MS.gene96681 | 0.869738 | 2.47E-66 | -1.69E-46 |
| MS.gene052178 | MS.gene96681 | 0.841752 | 3.93E-58 | -1.69E-46 |
| MS.gene052188 | MS.gene96681 | 0.809701 | 1.66E-50 | -1.69E-46 |
| MS.gene052253 | MS.gene96681 | 0.817765 | 2.78E-52 | -1.69E-46 |
| MS.gene052290 | MS.gene96681 | 0.831003 | 2.13E-55 | -1.69E-46 |
| MS.gene052310 | MS.gene96681 | 0.873552 | 1.35E-67 | -1.69E-46 |
| MS.gene052322 | MS.gene96681 | 0.812819 | 3.50E-51 | -1.69E-46 |
| MS.gene052326 | MS.gene96681 | 0.813245 | 2.82E-51 | -1.69E-46 |
| MS.gene052340 | MS.gene96681 | 0.858483 | 7.99E-63 | -1.69E-46 |
| MS.gene053073 | MS.gene96681 | 0.809488 | 1.85E-50 | -1.69E-46 |
| MS.gene053103 | MS.gene96681 | 0.814134 | 1.80E-51 | -1.69E-46 |
| MS.gene053330 | MS.gene96681 | 0.848364 | 6.43E-60 | -1.69E-46 |
| MS.gene053557 | MS.gene96681 | 0.846962 | 1.56E-59 | -1.69E-46 |
| MS.gene053836 | MS.gene96681 | 0.858122 | 1.02E-62 | -1.69E-46 |
| MS.gene053907 | MS.gene96681 | 0.868534 | 6.08E-66 | -1.69E-46 |
| MS.gene053928 | MS.gene96681 | 0.808645 | 2.80E-50 | -1.69E-46 |
| MS.gene053971 | MS.gene96681 | 0.913196 | 8.14E-84 | -1.69E-46 |
| MS.gene054123 | MS.gene96681 | 0.811135 | 8.14E-51 | -1.69E-46 |
| MS.gene054349 | MS.gene96681 | 0.80927 | 2.06E-50 | -1.69E-46 |
| MS.gene054918 | MS.gene96681 | 0.803001 | 4.29E-49 | -1.69E-46 |
| MS.gene054983 | MS.gene96681 | 0.841647 | 4.19E-58 | -1.69E-46 |
| MS.gene055116 | MS.gene96681 | 0.834104 | 3.63E-56 | -1.69E-46 |
| MS.gene055254 | MS.gene96681 | 0.810679 | 1.02E-50 | -1.69E-46 |
| MS.gene055277 | MS.gene96681 | 0.851097 | 1.11E-60 | -1.69E-46 |
| MS.gene055370 | MS.gene96681 | 0.839164 | 1.87E-57 | -1.69E-46 |
| MS.gene055422 | MS.gene96681 | 0.835664 | 1.47E-56 | -1.69E-46 |
| MS.gene055428 | MS.gene96681 | 0.874061 | 9.07E-68 | -1.69E-46 |
| MS.gene05553 | MS.gene96681 | 0.846299 | 2.37E-59 | -1.69E-46 |
| MS.gene055712 | MS.gene96681 | 0.811745 | 6.01E-51 | -1.69E-46 |
| MS.gene055980 | MS.gene96681 | 0.83721 | 5.95E-57 | -1.69E-46 |
| MS.gene056407 | MS.gene96681 | 0.824948 | 6.12E-54 | -1.69E-46 |
| MS.gene056451 | MS.gene96681 | 0.876923 | 9.49E-69 | -1.69E-46 |
| MS.gene056612 | MS.gene96681 | 0.87489 | 4.74E-68 | -1.69E-46 |
| MS.gene056633 | MS.gene96681 | 0.856557 | 2.97E-62 | -1.69E-46 |
| MS.gene056659 | MS.gene96681 | 0.804472 | 2.12E-49 | -1.69E-46 |
| MS.gene056664 | MS.gene96681 | 0.877643 | 5.33E-69 | -1.69E-46 |
| MS.gene056899 | MS.gene96681 | -0.833969 | 3.93E-56 | -1.69E-46 |
| MS.gene05702 | MS.gene96681 | 0.897537 | 1.28E-76 | -1.69E-46 |
| MS.gene057123 | MS.gene96681 | 0.82124 | 4.49E-53 | -1.69E-46 |
| MS.gene057295 | MS.gene96681 | 0.814049 | 1.88E-51 | -1.69E-46 |
| MS.gene05733 | MS.gene96681 | 0.826943 | 2.06E-54 | -1.69E-46 |
| MS.gene057439 | MS.gene96681 | 0.818791 | 1.63E-52 | -1.69E-46 |
| MS.gene058002 | MS.gene96681 | 0.849449 | 3.21E-60 | -1.69E-46 |
| MS.gene058082 | MS.gene96681 | 0.809753 | 1.62E-50 | -1.69E-46 |
| MS.gene05811 | MS.gene96681 | 0.81418 | 1.76E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene96681 | 0.842266 | 2.87E-58 | -1.69E-46 |
| MS.gene058233 | MS.gene96681 | 0.896427 | 3.73E-76 | -1.69E-46 |
| MS.gene058276 | MS.gene96681 | 0.883128 | 5.83E-71 | -1.69E-46 |
| MS.gene058293 | MS.gene96681 | 0.810532 | 1.10E-50 | -1.69E-46 |
| MS.gene058660 | MS.gene96681 | 0.823465 | 1.37E-53 | -1.69E-46 |
| MS.gene058746 | MS.gene96681 | 0.890464 | 9.62E-74 | -1.69E-46 |
| MS.gene058845 | MS.gene96681 | 0.802255 | 6.12E-49 | -1.69E-46 |
| MS.gene059011 | MS.gene96681 | 0.802445 | 5.59E-49 | -1.69E-46 |
| MS.gene05907 | MS.gene96681 | 0.833389 | 5.48E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene96681 | 0.809666 | 1.69E-50 | -1.69E-46 |
| MS.gene059326 | MS.gene96681 | 0.809029 | 2.32E-50 | -1.69E-46 |
| MS.gene059518 | MS.gene96681 | 0.824254 | 8.92E-54 | -1.69E-46 |
| MS.gene059597 | MS.gene96681 | 0.845126 | 4.93E-59 | -1.69E-46 |
| MS.gene059688 | MS.gene96681 | 0.814698 | 1.35E-51 | -1.69E-46 |
| MS.gene059692 | MS.gene96681 | 0.838831 | 2.28E-57 | -1.69E-46 |
| MS.gene059695 | MS.gene96681 | 0.837224 | 5.90E-57 | -1.69E-46 |
| MS.gene05984 | MS.gene96681 | 0.824363 | 8.41E-54 | -1.69E-46 |
| MS.gene059894 | MS.gene96681 | 0.908834 | 1.11E-81 | -1.69E-46 |
| MS.gene06037 | MS.gene96681 | 0.815806 | 7.65E-52 | -1.69E-46 |
| MS.gene060403 | MS.gene96681 | 0.804215 | 2.40E-49 | -1.69E-46 |
| MS.gene060407 | MS.gene96681 | 0.871752 | 5.38E-67 | -1.69E-46 |
| MS.gene06090 | MS.gene96681 | 0.802697 | 4.96E-49 | -1.69E-46 |
| MS.gene061049 | MS.gene96681 | 0.904755 | 8.81E-80 | -1.69E-46 |
| MS.gene061112 | MS.gene96681 | 0.848444 | 6.11E-60 | -1.69E-46 |
| MS.gene061159 | MS.gene96681 | 0.805231 | 1.47E-49 | -1.69E-46 |
| MS.gene061265 | MS.gene96681 | 0.841843 | 3.72E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96681.t1 | MTR_6g051780 | 94.536 | 183 | 10 | 0 | 1 | 183 | 1 | 183 | 2.10e-112 | 318 |
| MS.gene96681.t1 | MTR_5g027010 | 34.356 | 163 | 90 | 3 | 29 | 176 | 33 | 193 | 6.26e-23 | 90.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene96681.t1 | AT5G52780 | 50.000 | 94 | 47 | 0 | 85 | 178 | 74 | 167 | 1.72e-30 | 109 |
| MS.gene96681.t1 | AT4G19100 | 43.564 | 101 | 57 | 0 | 83 | 183 | 49 | 149 | 3.49e-22 | 87.8 |
| MS.gene96681.t1 | AT4G19100 | 43.564 | 101 | 57 | 0 | 83 | 183 | 112 | 212 | 4.53e-22 | 89.4 |
Find 0 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAGTTATGTATAGAATAAT+CGG | - | 43126:3066-3085 | MS.gene96681:CDS | 20.0% |
| !!! | TTGAATTGTTTTGATGTATT+GGG | + | 43126:2904-2923 | MS.gene96681:intergenic | 20.0% |
| ! | ATCATCATCATTCTTATTGT+TGG | + | 43126:3034-3053 | MS.gene96681:intergenic | 25.0% |
| ! | ATTCTATACATAACTTCCTT+AGG | + | 43126:3063-3082 | MS.gene96681:intergenic | 25.0% |
| ! | TCATCATCATTCTTATTGTT+GGG | + | 43126:3033-3052 | MS.gene96681:intergenic | 25.0% |
| !!! | ATGTATTGGGTTGAAAAAAT+GGG | + | 43126:2891-2910 | MS.gene96681:intergenic | 25.0% |
| !!! | GTTGAATTGTTTTGATGTAT+TGG | + | 43126:2905-2924 | MS.gene96681:intergenic | 25.0% |
| AAATATTTGATGCTCCTTTG+TGG | - | 43126:3169-3188 | MS.gene96681:CDS | 30.0% | |
| AATACAAGAGTTGTCACAAA+TGG | + | 43126:3198-3217 | MS.gene96681:intergenic | 30.0% | |
| ACCATCAACAAATAAAGCTA+AGG | - | 43126:2984-3003 | MS.gene96681:CDS | 30.0% | |
| TGAAGAAGTTGAGAAGAATT+GGG | - | 43126:3302-3321 | MS.gene96681:CDS | 30.0% | |
| TTATCATTCCTGCATCTTTA+TGG | - | 43126:3129-3148 | MS.gene96681:CDS | 30.0% | |
| TTGAAGAAGTTGAGAAGAAT+TGG | - | 43126:3301-3320 | MS.gene96681:CDS | 30.0% | |
| ! | GTTTAGTACTTTAGTTCCAA+TGG | - | 43126:3098-3117 | MS.gene96681:CDS | 30.0% |
| ! | TGAAAAAATGGGAAGTTTTG+TGG | + | 43126:2880-2899 | MS.gene96681:intergenic | 30.0% |
| ! | TGATGATGATGATATTCCTA+AGG | - | 43126:3044-3063 | MS.gene96681:CDS | 30.0% |
| !! | AAGTTTTGTGGTTTACGTAT+TGG | + | 43126:2868-2887 | MS.gene96681:intergenic | 30.0% |
| !! | GATGTATTGGGTTGAAAAAA+TGG | + | 43126:2892-2911 | MS.gene96681:intergenic | 30.0% |
| !! | TCCTTAGCTTTATTTGTTGA+TGG | + | 43126:2988-3007 | MS.gene96681:intergenic | 30.0% |
| !! | TTAGCTTTATTTGTTGATGG+TGG | + | 43126:2985-3004 | MS.gene96681:intergenic | 30.0% |
| !!! | GTGACAACTCTTGTATTTTT+TGG | - | 43126:3201-3220 | MS.gene96681:CDS | 30.0% |
| AATGATAACCCTAATGCCAT+TGG | + | 43126:3117-3136 | MS.gene96681:intergenic | 35.0% | |
| TTAACTCACCATAAAGATGC+AGG | + | 43126:3140-3159 | MS.gene96681:intergenic | 35.0% | |
| ! | ACTTTAGTTCCAATGGCATT+AGG | - | 43126:3105-3124 | MS.gene96681:CDS | 35.0% |
| ! | CTTTAGTTCCAATGGCATTA+GGG | - | 43126:3106-3125 | MS.gene96681:CDS | 35.0% |
| !!! | CTTGTATTTTTTGGAGCTTC+TGG | - | 43126:3210-3229 | MS.gene96681:CDS | 35.0% |
| AGAGAGAGAAGGTTCTTTGT+TGG | - | 43126:3275-3294 | MS.gene96681:CDS | 40.0% | |
| GAGAGAGAAGGTTCTTTGTT+GGG | - | 43126:3276-3295 | MS.gene96681:CDS | 40.0% | |
| GTGTTGAGCTATGGAGAATT+GGG | + | 43126:2840-2859 | MS.gene96681:intergenic | 40.0% | |
| TCTGGACTTGGAATTGCATA+TGG | - | 43126:3228-3247 | MS.gene96681:CDS | 40.0% | |
| TGTGTTGAGCTATGGAGAAT+TGG | + | 43126:2841-2860 | MS.gene96681:intergenic | 40.0% | |
| TTGGAGATTGTGTTGAGCTA+TGG | + | 43126:2849-2868 | MS.gene96681:intergenic | 40.0% | |
| ! | TGGTGTGCTTTCTTCTAGTT+TGG | - | 43126:3248-3267 | MS.gene96681:CDS | 40.0% |
| !! | AGAAGAATTGGGTTGAGATG+TGG | - | 43126:3313-3332 | MS.gene96681:CDS | 40.0% |
| !!! | TTTTTTGGAGCTTCTGGACT+TGG | - | 43126:3216-3235 | MS.gene96681:CDS | 40.0% |
| AGTTTGGATGCAGAGAGAGA+AGG | - | 43126:3264-3283 | MS.gene96681:CDS | 45.0% | |
| GTCACAAATGGCATCCACAA+AGG | + | 43126:3186-3205 | MS.gene96681:intergenic | 45.0% | |
| TCTTGTGTTGGAGAAGCCTT+TGG | + | 43126:2962-2981 | MS.gene96681:intergenic | 45.0% | |
| !! | TGTTGATGGTGGTCTTGTGT+TGG | + | 43126:2974-2993 | MS.gene96681:intergenic | 45.0% |
| AAGCCACAAGCTAGTGCCAA+AGG | - | 43126:2943-2962 | MS.gene96681:CDS | 50.0% | |
| GTGGCTTCAACGTGGTGAAT+TGG | + | 43126:2930-2949 | MS.gene96681:intergenic | 50.0% | |
| ! | AAGCCTTTGGCACTAGCTTG+TGG | + | 43126:2949-2968 | MS.gene96681:intergenic | 50.0% |
| ! | ACTAGCTTGTGGCTTCAACG+TGG | + | 43126:2938-2957 | MS.gene96681:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 43126 | gene | 2811 | 3362 | 2811 | ID=MS.gene96681 |
| 43126 | mRNA | 2811 | 3362 | 2811 | ID=MS.gene96681.t1;Parent=MS.gene96681 |
| 43126 | exon | 2811 | 3362 | 2811 | ID=MS.gene96681.t1.exon1;Parent=MS.gene96681.t1 |
| 43126 | CDS | 2811 | 3362 | 2811 | ID=cds.MS.gene96681.t1;Parent=MS.gene96681.t1 |
| Gene Sequence |
| Protein sequence |
