AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07055


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07055.t1 MTR_7g109520 96.094 128 4 1 1 127 1 128 6.92e-88 251
MS.gene07055.t1 MTR_1g087030 64.615 130 41 3 2 127 3 131 6.47e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07055.t1 AT3G62410 58.647 133 47 5 1 127 1 131 1.48e-45 144
MS.gene07055.t1 AT2G47400 68.041 97 27 2 33 127 30 124 4.72e-40 130
MS.gene07055.t1 AT1G76560 47.297 74 37 1 56 127 61 134 6.66e-19 77.0

Find 38 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTGATATCTGTTC+TGG 0.263996 7.2:+5679411 None:intergenic
TGTTGTTGGTGAGATCTTGA+TGG 0.363034 7.2:+5679503 None:intergenic
GCTACGCATTCTCCACTAAC+CGG 0.387490 7.2:+5679345 None:intergenic
AAGCTTCTCTTCAACTCATC+AGG 0.395970 7.2:-5679537 MS.gene07055:CDS
GATGAGTTGAAGAGAAGCTT+GGG 0.422285 7.2:+5679540 None:intergenic
TGTTCTGGTGCAGCACGAAC+TGG 0.440452 7.2:+5679426 None:intergenic
CACCATCATTCGACCTGATC+CGG 0.449625 7.2:+5679455 None:intergenic
CGGTGGGGTGTAGCCGGATC+AGG 0.450264 7.2:-5679468 MS.gene07055:CDS
CGTGTCTGCAGTGGTGTTGT+TGG 0.454736 7.2:+5679489 None:intergenic
GGTGAGATCTTGATGGTTTG+TGG 0.456742 7.2:+5679510 None:intergenic
TGTGCTGATGACCCGGTTAG+TGG 0.494167 7.2:-5679357 MS.gene07055:CDS
GAAGAAGGATTCTGATCCAT+TGG 0.495582 7.2:-5679268 MS.gene07055:CDS
CGGCGAGTCATGCAAGGGAT+AGG 0.497910 7.2:-5679290 MS.gene07055:CDS
TGGAGAATGCGTAGCAGCAT+GGG 0.517025 7.2:-5679337 MS.gene07055:CDS
CAGACACGGTGGGGTGTAGC+CGG 0.517241 7.2:-5679474 MS.gene07055:CDS
TGATGAGTTGAAGAGAAGCT+TGG 0.520626 7.2:+5679539 None:intergenic
AGAGAAGCTTGGGGTTTGAA+AGG 0.525586 7.2:+5679550 None:intergenic
TCCATTGGAGGATTACTGTA+AGG 0.532128 7.2:-5679253 MS.gene07055:CDS
GTGCTGCGGCGAGTCATGCA+AGG 0.540725 7.2:-5679296 MS.gene07055:CDS
GTGGAGAATGCGTAGCAGCA+TGG 0.561567 7.2:-5679338 MS.gene07055:CDS
CGTAGCAGCATGGGATGAAG+TGG 0.567468 7.2:-5679328 MS.gene07055:CDS
TCATGCAAGGGATAGGAAGA+AGG 0.572718 7.2:-5679283 MS.gene07055:CDS
ACCAGAACAGATATCAAAGA+AGG 0.574036 7.2:-5679412 MS.gene07055:CDS
AGAGACATGTGCTGATGACC+CGG 0.581174 7.2:-5679364 MS.gene07055:CDS
TCCTTACAGTAATCCTCCAA+TGG 0.581674 7.2:+5679252 None:intergenic
CTACGCATTCTCCACTAACC+GGG 0.586814 7.2:+5679346 None:intergenic
AGCCGGATCAGGTCGAATGA+TGG 0.587328 7.2:-5679457 MS.gene07055:CDS
CAACAACACCACTGCAGACA+CGG 0.593172 7.2:-5679488 MS.gene07055:CDS
GAAGGATTCTGATCCATTGG+AGG 0.594880 7.2:-5679265 MS.gene07055:CDS
ACACCCCACCGTGTCTGCAG+TGG 0.598200 7.2:+5679480 None:intergenic
AACACCACTGCAGACACGGT+GGG 0.598529 7.2:-5679484 MS.gene07055:CDS
ACACCACTGCAGACACGGTG+GGG 0.612423 7.2:-5679483 MS.gene07055:CDS
CGGATCAGGTCGAATGATGG+TGG 0.612425 7.2:-5679454 MS.gene07055:CDS
AGCAGCATGGGATGAAGTGG+AGG 0.620459 7.2:-5679325 MS.gene07055:CDS
TGCTGCGGCGAGTCATGCAA+GGG 0.625380 7.2:-5679295 MS.gene07055:CDS
CAACACCACTGCAGACACGG+TGG 0.673443 7.2:-5679485 MS.gene07055:CDS
ATGAGTTGAAGAGAAGCTTG+GGG 0.682917 7.2:+5679541 None:intergenic
AGTGGAGGAACTCAGTGCTG+CGG 0.688380 7.2:-5679310 MS.gene07055:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACCAGAACAGATATCAAAGA+AGG - chr7.2:5679373-5679392 MS.gene07055:CDS 35.0%
ACCTTCTTTGATATCTGTTC+TGG + chr7.2:5679377-5679396 None:intergenic 35.0%
AAGCTTCTCTTCAACTCATC+AGG - chr7.2:5679248-5679267 MS.gene07055:CDS 40.0%
GAAGAAGGATTCTGATCCAT+TGG - chr7.2:5679517-5679536 MS.gene07055:CDS 40.0%
TCCATTGGAGGATTACTGTA+AGG - chr7.2:5679532-5679551 MS.gene07055:CDS 40.0%
TCCTTACAGTAATCCTCCAA+TGG + chr7.2:5679536-5679555 None:intergenic 40.0%
! ATGAGTTGAAGAGAAGCTTG+GGG + chr7.2:5679247-5679266 None:intergenic 40.0%
! GATGAGTTGAAGAGAAGCTT+GGG + chr7.2:5679248-5679267 None:intergenic 40.0%
! TGATGAGTTGAAGAGAAGCT+TGG + chr7.2:5679249-5679268 None:intergenic 40.0%
! TGTTGTTGGTGAGATCTTGA+TGG + chr7.2:5679285-5679304 None:intergenic 40.0%
! TTTTGCACTCATCAGTCTCA+GGG + chr7.2:5679562-5679581 None:intergenic 40.0%
AGAGAAGCTTGGGGTTTGAA+AGG + chr7.2:5679238-5679257 None:intergenic 45.0%
GAAGGATTCTGATCCATTGG+AGG - chr7.2:5679520-5679539 MS.gene07055:CDS 45.0%
TCATGCAAGGGATAGGAAGA+AGG - chr7.2:5679502-5679521 MS.gene07055:CDS 45.0%
! GGTGAGATCTTGATGGTTTG+TGG + chr7.2:5679278-5679297 None:intergenic 45.0%
! GTTTTGCACTCATCAGTCTC+AGG + chr7.2:5679563-5679582 None:intergenic 45.0%
CAACAACACCACTGCAGACA+CGG - chr7.2:5679297-5679316 MS.gene07055:CDS 50.0%
CACCATCATTCGACCTGATC+CGG + chr7.2:5679333-5679352 None:intergenic 50.0%
CTACGCATTCTCCACTAACC+GGG + chr7.2:5679442-5679461 None:intergenic 50.0%
GCTACGCATTCTCCACTAAC+CGG + chr7.2:5679443-5679462 None:intergenic 50.0%
TGGAGAATGCGTAGCAGCAT+GGG - chr7.2:5679448-5679467 MS.gene07055:CDS 50.0%
! AGAGACATGTGCTGATGACC+CGG - chr7.2:5679421-5679440 MS.gene07055:CDS 50.0%
AACACCACTGCAGACACGGT+GGG - chr7.2:5679301-5679320 MS.gene07055:CDS 55.0%
AGCAGCATGGGATGAAGTGG+AGG - chr7.2:5679460-5679479 MS.gene07055:CDS 55.0%
AGCCGGATCAGGTCGAATGA+TGG - chr7.2:5679328-5679347 MS.gene07055:CDS 55.0%
AGTGGAGGAACTCAGTGCTG+CGG - chr7.2:5679475-5679494 MS.gene07055:CDS 55.0%
CGGATCAGGTCGAATGATGG+TGG - chr7.2:5679331-5679350 MS.gene07055:CDS 55.0%
CGTAGCAGCATGGGATGAAG+TGG - chr7.2:5679457-5679476 MS.gene07055:CDS 55.0%
GTGGAGAATGCGTAGCAGCA+TGG - chr7.2:5679447-5679466 MS.gene07055:CDS 55.0%
TGTTCTGGTGCAGCACGAAC+TGG + chr7.2:5679362-5679381 None:intergenic 55.0%
! TGTGCTGATGACCCGGTTAG+TGG - chr7.2:5679428-5679447 MS.gene07055:CDS 55.0%
!! CGTGTCTGCAGTGGTGTTGT+TGG + chr7.2:5679299-5679318 None:intergenic 55.0%
ACACCACTGCAGACACGGTG+GGG - chr7.2:5679302-5679321 MS.gene07055:CDS 60.0%
CAACACCACTGCAGACACGG+TGG - chr7.2:5679300-5679319 MS.gene07055:CDS 60.0%
CGGCGAGTCATGCAAGGGAT+AGG - chr7.2:5679495-5679514 MS.gene07055:CDS 60.0%
TGCTGCGGCGAGTCATGCAA+GGG - chr7.2:5679490-5679509 MS.gene07055:CDS 60.0%
ACACCCCACCGTGTCTGCAG+TGG + chr7.2:5679308-5679327 None:intergenic 65.0%
CAGACACGGTGGGGTGTAGC+CGG - chr7.2:5679311-5679330 MS.gene07055:CDS 65.0%
GTGCTGCGGCGAGTCATGCA+AGG - chr7.2:5679489-5679508 MS.gene07055:CDS 65.0%
CGGTGGGGTGTAGCCGGATC+AGG - chr7.2:5679317-5679336 MS.gene07055:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 5679212 5679595 5679212 ID=MS.gene07055
chr7.2 mRNA 5679212 5679595 5679212 ID=MS.gene07055.t1;Parent=MS.gene07055
chr7.2 exon 5679212 5679595 5679212 ID=MS.gene07055.t1.exon1;Parent=MS.gene07055.t1
chr7.2 CDS 5679212 5679595 5679212 ID=cds.MS.gene07055.t1;Parent=MS.gene07055.t1
Gene Sequence

>MS.gene07055

ATGGCAACAATAGGTGGTCTCAGCCTTTCAAACCCCAAGCTTCTCTTCAACTCATCAGGATTTCCACAAACCATCAAGATCTCACCAACAACACCACTGCAGACACGGTGGGGTGTAGCCGGATCAGGTCGAATGATGGTGGTTCGACCAGTTCGTGCTGCACCAGAACAGATATCAAAGAAGGTAGAAGAAAGCATTAAGAGTGCGCAAGAGACATGTGCTGATGACCCGGTTAGTGGAGAATGCGTAGCAGCATGGGATGAAGTGGAGGAACTCAGTGCTGCGGCGAGTCATGCAAGGGATAGGAAGAAGGATTCTGATCCATTGGAGGATTACTGTAAGGATAACCCTGAGACTGATGAGTGCAAAACTTATGATAACTGA

Protein sequence

>MS.gene07055.t1

MATIGGLSLSNPKLLFNSSGFPQTIKISPTTPLQTRWGVAGSGRMMVVRPVRAAPEQISKKVEESIKSAQETCADDPVSGECVAAWDEVEELSAAASHARDRKKDSDPLEDYCKDNPETDECKTYDN