AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene37678


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37678.t1 MTR_1g102510 86.260 262 9 2 1 235 1 262 1.92e-153 427
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene37678.t1 AT5G51680 29.080 337 131 11 4 235 10 343 5.35e-15 73.6

Find 52 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACCTTCAATTCCTTTCATT+TGG 0.224391 1.1:-73575726 MS.gene37678:CDS
AAGAAAGGAAGAAACATATT+AGG 0.233439 1.1:-73575753 MS.gene37678:CDS
TTGCCTCAATTCCCTTTGTT+TGG 0.279099 1.1:-73575618 MS.gene37678:CDS
TGAAAAGTTAAGTTCAAGTT+TGG 0.340393 1.1:-73575260 MS.gene37678:CDS
ACCTTCAATTCCTTTCATTT+GGG 0.345813 1.1:-73575725 MS.gene37678:CDS
TATCAAAACTAAATGATTCT+AGG 0.360427 1.1:+73575524 None:intergenic
ACCACGAGAAAGCAAGCTTT+TGG 0.370273 1.1:-73574791 MS.gene37678:CDS
TGCCTCAATTCCCTTTGTTT+GGG 0.372035 1.1:-73575617 MS.gene37678:CDS
AAGCAACTCAAATGAACAAA+TGG 0.378323 1.1:-73575123 MS.gene37678:CDS
TTCAAGTTTGGTGGAACTAA+AGG 0.388380 1.1:-73575248 MS.gene37678:CDS
AACTGAGTTAAATGATTCAT+TGG 0.396407 1.1:+73575501 None:intergenic
AGTATAAGCAGCTATGAAAC+TGG 0.406907 1.1:-73575376 MS.gene37678:CDS
AAACTAGGGAGGGGTATTCC+AGG 0.409764 1.1:+73575586 None:intergenic
TTCTAGGTCCAAGCTAGTTA+TGG 0.418005 1.1:+73575540 None:intergenic
AGAAAGGAAGAAACATATTA+GGG 0.423493 1.1:-73575752 MS.gene37678:CDS
ATGGAACAAGTTACCTTTGG+TGG 0.430078 1.1:+73575097 None:intergenic
TCAAGTTTGGTGGAACTAAA+GGG 0.439502 1.1:-73575247 MS.gene37678:CDS
CAGGCATTCCTATAAAAGAT+TGG 0.447195 1.1:-73575693 MS.gene37678:CDS
AAAGTTAAGTTCAAGTTTGG+TGG 0.448826 1.1:-73575257 MS.gene37678:CDS
CTCTCCAGCTTCGTCGGAGA+TGG 0.451790 1.1:-73575404 MS.gene37678:CDS
TTCCCAAACAAAGGGAATTG+AGG 0.478406 1.1:+73575615 None:intergenic
CTTTAGACTAGTTTCAGCCT+AGG 0.480571 1.1:+73574707 None:intergenic
AGAGAGTTTGCAAGTAAAGA+AGG 0.480592 1.1:+73575478 None:intergenic
CTATCCATCTCCGACGAAGC+TGG 0.483008 1.1:+73575400 None:intergenic
TTTAGACTAGTTTCAGCCTA+GGG 0.498911 1.1:+73574708 None:intergenic
TCCCAAATGAAAGGAATTGA+AGG 0.504204 1.1:+73575724 None:intergenic
AGCAACTCAAATGAACAAAT+GGG 0.509084 1.1:-73575122 MS.gene37678:CDS
TCATCAACATGTGAGAAACT+AGG 0.514045 1.1:+73575571 None:intergenic
ACAAGCAGCAGTATCATTGA+AGG 0.522761 1.1:-73574752 MS.gene37678:CDS
TCCGACGAAGCTGGAGAGGA+TGG 0.522845 1.1:+73575409 None:intergenic
ATAATTAACTCTCCAAGTGT+TGG 0.527351 1.1:+73575175 None:intergenic
GAGGATGGAGTTTGCTGTAG+TGG 0.531543 1.1:+73575424 None:intergenic
TCCATCCTCTCCAGCTTCGT+CGG 0.537624 1.1:-73575410 MS.gene37678:CDS
TTCATTTGGGAAACAAAACC+AGG 0.538522 1.1:-73575712 MS.gene37678:CDS
TCAAGGAAGCAACCAACACT+TGG 0.542435 1.1:-73575187 MS.gene37678:CDS
TTGCTTCCTTGACATATCAC+AGG 0.543111 1.1:+73575198 None:intergenic
TCCAAAAGCTTGCTTTCTCG+TGG 0.546204 1.1:+73574790 None:intergenic
CATCAACATGTGAGAAACTA+GGG 0.549198 1.1:+73575572 None:intergenic
ATTATACCTGTGATATGTCA+AGG 0.573360 1.1:-73575204 MS.gene37678:CDS
GAGTGAAACCATAACTAGCT+TGG 0.574998 1.1:-73575548 MS.gene37678:CDS
TGACAAAATGATGAGTCCAC+TGG 0.586915 1.1:+73575667 None:intergenic
TGAAGAGAAAGTACTACCCT+AGG 0.596271 1.1:-73574724 MS.gene37678:CDS
CATCTCCGACGAAGCTGGAG+AGG 0.596381 1.1:+73575405 None:intergenic
TGGAGTTTGCTGTAGTGGCA+TGG 0.598354 1.1:+73575429 None:intergenic
CAAATGAAAGGAATTGAAGG+TGG 0.607064 1.1:+73575727 None:intergenic
GAACAAATGGGATCCACCAA+AGG 0.609336 1.1:-73575110 MS.gene37678:intron
ACATGTGAGAAACTAGGGAG+GGG 0.616121 1.1:+73575577 None:intergenic
TAAGCAGCTATGAAACTGGA+AGG 0.618972 1.1:-73575372 MS.gene37678:intron
TATAAAAGATTGGAAGCCAG+TGG 0.619766 1.1:-73575683 MS.gene37678:CDS
AACATGTGAGAAACTAGGGA+GGG 0.639709 1.1:+73575576 None:intergenic
CAAGCAGCAGTATCATTGAA+GGG 0.643098 1.1:-73574751 MS.gene37678:CDS
CAACATGTGAGAAACTAGGG+AGG 0.699559 1.1:+73575575 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACGTTTATTGACATAAAAA+TGG + chr1.1:73575397-73575416 None:intergenic 20.0%
!! ATTCAAATTAGCATCTAAAT+TGG - chr1.1:73575556-73575575 MS.gene37678:CDS 20.0%
!! TATCAAAACTAAATGATTCT+AGG + chr1.1:73574951-73574970 None:intergenic 20.0%
!!! AATTGGTTACTTTTTAAGTA+TGG - chr1.1:73575573-73575592 MS.gene37678:CDS 20.0%
! AACTGAGTTAAATGATTCAT+TGG + chr1.1:73574974-73574993 None:intergenic 25.0%
! AAGAAAGGAAGAAACATATT+AGG - chr1.1:73574719-73574738 MS.gene37678:CDS 25.0%
! AGAAAGGAAGAAACATATTA+GGG - chr1.1:73574720-73574739 MS.gene37678:CDS 25.0%
! ATAATCTCATCGAGAAAAAT+TGG + chr1.1:73575538-73575557 None:intergenic 25.0%
! CTAAAACGTTGAATTAAGAT+CGG - chr1.1:73575455-73575474 MS.gene37678:CDS 25.0%
! TATGTCAATAAACGTTTCTT+TGG - chr1.1:73575401-73575420 MS.gene37678:CDS 25.0%
!! TGAAAAGTTAAGTTCAAGTT+TGG - chr1.1:73575212-73575231 MS.gene37678:CDS 25.0%
!!! ATTTGTTTTAGTGGAGTTTT+TGG + chr1.1:73574835-73574854 None:intergenic 25.0%
AAAATGGAACAAGTTACCTT+TGG + chr1.1:73575381-73575400 None:intergenic 30.0%
AAGCAACTCAAATGAACAAA+TGG - chr1.1:73575349-73575368 MS.gene37678:intron 30.0%
ACCTTCAATTCCTTTCATTT+GGG - chr1.1:73574747-73574766 MS.gene37678:CDS 30.0%
AGCAACTCAAATGAACAAAT+GGG - chr1.1:73575350-73575369 MS.gene37678:intron 30.0%
ATAATTAACTCTCCAAGTGT+TGG + chr1.1:73575300-73575319 None:intergenic 30.0%
ATTATACCTGTGATATGTCA+AGG - chr1.1:73575268-73575287 MS.gene37678:CDS 30.0%
TGAAGCAAAAGAGTAAATGT+TGG + chr1.1:73575650-73575669 None:intergenic 30.0%
! AAAGTTAAGTTCAAGTTTGG+TGG - chr1.1:73575215-73575234 MS.gene37678:CDS 30.0%
! GACTTTGTATTTGGAATACT+TGG + chr1.1:73575505-73575524 None:intergenic 30.0%
!!! AAAGTCTGGTTTTCTTGAAA+GGG - chr1.1:73575176-73575195 MS.gene37678:CDS 30.0%
!!! ATTGAGGCAATTTGTTTTAG+TGG + chr1.1:73574844-73574863 None:intergenic 30.0%
!!! TTTAACTGGTTCTGCTTTTT+TGG - chr1.1:73575128-73575147 MS.gene37678:CDS 30.0%
AGAGAGTTTGCAAGTAAAGA+AGG + chr1.1:73574997-73575016 None:intergenic 35.0%
AGTATAAGCAGCTATGAAAC+TGG - chr1.1:73575096-73575115 MS.gene37678:intron 35.0%
CAAATGAAAGGAATTGAAGG+TGG + chr1.1:73574748-73574767 None:intergenic 35.0%
CACCTTCAATTCCTTTCATT+TGG - chr1.1:73574746-73574765 MS.gene37678:CDS 35.0%
CAGGCATTCCTATAAAAGAT+TGG - chr1.1:73574779-73574798 MS.gene37678:CDS 35.0%
CATCAACATGTGAGAAACTA+GGG + chr1.1:73574903-73574922 None:intergenic 35.0%
CTTTCAAGAAAACCAGACTT+TGG + chr1.1:73575177-73575196 None:intergenic 35.0%
TATAAAAGATTGGAAGCCAG+TGG - chr1.1:73574789-73574808 MS.gene37678:CDS 35.0%
TCATCAACATGTGAGAAACT+AGG + chr1.1:73574904-73574923 None:intergenic 35.0%
TCCCAAATGAAAGGAATTGA+AGG + chr1.1:73574751-73574770 None:intergenic 35.0%
TCGTAGTCTGACTTTGTATT+TGG + chr1.1:73575514-73575533 None:intergenic 35.0%
TTCATTTGGGAAACAAAACC+AGG - chr1.1:73574760-73574779 MS.gene37678:CDS 35.0%
TTTGTTTGGGAAGAAAAACC+TGG - chr1.1:73574868-73574887 MS.gene37678:intron 35.0%
! AGGTTTTTCTTCCCAAACAA+AGG + chr1.1:73574869-73574888 None:intergenic 35.0%
! CAATCTTTTATAGGAATGCC+TGG + chr1.1:73574781-73574800 None:intergenic 35.0%
! GGTTTTCTTGAAAGGGATAT+TGG - chr1.1:73575183-73575202 MS.gene37678:CDS 35.0%
! GGTTTTTCTTCCCAAACAAA+GGG + chr1.1:73574868-73574887 None:intergenic 35.0%
! TCAAGTTTGGTGGAACTAAA+GGG - chr1.1:73575225-73575244 MS.gene37678:CDS 35.0%
! TTCAAGTTTGGTGGAACTAA+AGG - chr1.1:73575224-73575243 MS.gene37678:CDS 35.0%
!! ACTGGCTTCCAATCTTTTAT+AGG + chr1.1:73574790-73574809 None:intergenic 35.0%
!!! CAAAGTCTGGTTTTCTTGAA+AGG - chr1.1:73575175-73575194 MS.gene37678:CDS 35.0%
!!! GGTTTTGTTTCCCAAATGAA+AGG + chr1.1:73574760-73574779 None:intergenic 35.0%
AACATGTGAGAAACTAGGGA+GGG + chr1.1:73574899-73574918 None:intergenic 40.0%
ACAAGCAGCAGTATCATTGA+AGG - chr1.1:73575720-73575739 MS.gene37678:CDS 40.0%
ACTGGAAGGTCAAGTTTAAC+TGG - chr1.1:73575114-73575133 MS.gene37678:CDS 40.0%
ATGGAACAAGTTACCTTTGG+TGG + chr1.1:73575378-73575397 None:intergenic 40.0%
CAAGCAGCAGTATCATTGAA+GGG - chr1.1:73575721-73575740 MS.gene37678:CDS 40.0%
GAGTGAAACCATAACTAGCT+TGG - chr1.1:73574924-73574943 MS.gene37678:intron 40.0%
TAAGCAGCTATGAAACTGGA+AGG - chr1.1:73575100-73575119 MS.gene37678:intron 40.0%
TCAAGAAAACCAGACTTTGG+TGG + chr1.1:73575174-73575193 None:intergenic 40.0%
TGACAAAATGATGAGTCCAC+TGG + chr1.1:73574808-73574827 None:intergenic 40.0%
TTCCCAAACAAAGGGAATTG+AGG + chr1.1:73574860-73574879 None:intergenic 40.0%
TTCTAGGTCCAAGCTAGTTA+TGG + chr1.1:73574935-73574954 None:intergenic 40.0%
TTGCCTCAATTCCCTTTGTT+TGG - chr1.1:73574854-73574873 MS.gene37678:intron 40.0%
TTGCTTCCTTGACATATCAC+AGG + chr1.1:73575277-73575296 None:intergenic 40.0%
! TGCCTCAATTCCCTTTGTTT+GGG - chr1.1:73574855-73574874 MS.gene37678:intron 40.0%
!! TGAAGAGAAAGTACTACCCT+AGG - chr1.1:73575748-73575767 MS.gene37678:CDS 40.0%
ACATGTGAGAAACTAGGGAG+GGG + chr1.1:73574898-73574917 None:intergenic 45.0%
CAACATGTGAGAAACTAGGG+AGG + chr1.1:73574900-73574919 None:intergenic 45.0%
CCAGACTTTGGTGGAAACAA+AGG + chr1.1:73575165-73575184 None:intergenic 45.0%
GAACAAATGGGATCCACCAA+AGG - chr1.1:73575362-73575381 MS.gene37678:intron 45.0%
TCAAGGAAGCAACCAACACT+TGG - chr1.1:73575285-73575304 MS.gene37678:intron 45.0%
! ACCACGAGAAAGCAAGCTTT+TGG - chr1.1:73575681-73575700 MS.gene37678:CDS 45.0%
! CCTTTGTTTCCACCAAAGTC+TGG - chr1.1:73575162-73575181 MS.gene37678:CDS 45.0%
! TCCAAAAGCTTGCTTTCTCG+TGG + chr1.1:73575685-73575704 None:intergenic 45.0%
!! AAACTAGGGAGGGGTATTCC+AGG + chr1.1:73574889-73574908 None:intergenic 50.0%
!! GAGGATGGAGTTTGCTGTAG+TGG + chr1.1:73575051-73575070 None:intergenic 50.0%
!! TGGAGTTTGCTGTAGTGGCA+TGG + chr1.1:73575046-73575065 None:intergenic 50.0%
CTATCCATCTCCGACGAAGC+TGG + chr1.1:73575075-73575094 None:intergenic 55.0%
TCCATCCTCTCCAGCTTCGT+CGG - chr1.1:73575062-73575081 MS.gene37678:intron 55.0%
CATCTCCGACGAAGCTGGAG+AGG + chr1.1:73575070-73575089 None:intergenic 60.0%
CTCTCCAGCTTCGTCGGAGA+TGG - chr1.1:73575068-73575087 MS.gene37678:intron 60.0%
TCCGACGAAGCTGGAGAGGA+TGG + chr1.1:73575066-73575085 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr1.1 gene 73574709 73575785 73574709 ID=MS.gene37678
chr1.1 mRNA 73574709 73575785 73574709 ID=MS.gene37678.t1;Parent=MS.gene37678
chr1.1 exon 73575373 73575785 73575373 ID=MS.gene37678.t1.exon1;Parent=MS.gene37678.t1
chr1.1 CDS 73575373 73575785 73575373 ID=cds.MS.gene37678.t1;Parent=MS.gene37678.t1
chr1.1 exon 73575111 73575297 73575111 ID=MS.gene37678.t1.exon2;Parent=MS.gene37678.t1
chr1.1 CDS 73575111 73575297 73575111 ID=cds.MS.gene37678.t1;Parent=MS.gene37678.t1
chr1.1 exon 73574709 73574816 73574709 ID=MS.gene37678.t1.exon3;Parent=MS.gene37678.t1
chr1.1 CDS 73574709 73574816 73574709 ID=cds.MS.gene37678.t1;Parent=MS.gene37678.t1
Gene Sequence

>MS.gene37678

ATGAATGAAAAAGAAAGGAAGAAACATATTAGGGAACCACCTTCAATTCCTTTCATTTGGGAAACAAAACCAGGCATTCCTATAAAAGATTGGAAGCCAGTGGACTCATCATTTTGTCAATTACCAAAAACTCCACTAAAACAAATTGCCTCAATTCCCTTTGTTTGGGAAGAAAAACCTGGAATACCCCTCCCTAGTTTCTCACATGTTGATGAGAGTGAAACCATAACTAGCTTGGACCTAGAATCATTTAGTTTTGATACCAATGAATCATTTAACTCAGTTCCTTCTTTACTTGCAAACTCTCTTGTATCATCAACTAAACTCTCTACAGCCATGCCACTACAGCAAACTCCATCCTCTCCAGCTTCGTCGGAGATGGATAGTAGTATAAGCAGCTATGAAACTGGAAGGTCAAGTTTAACTGGTTCTGCTTTTTTGGAATGTCTATTTCCTTTGTTTCCACCAAAGTCTGGTTTTCTTGAAAGGGATATTGGTTACAATGAAAAGTTAAGTTCAAGTTTGGTGGAACTAAAGGGAAATGATTTTGAGAATGAAGATTATACCTGTGATATGTCAAGGAAGCAACCAACACTTGGAGAGTTAATTATGATGAGCAGAAGAAGAAGCTGCATAAGAAAAGCAACTCAAATGAACAAATGGGATCCACCAAAGGTAACTTGTTCCATTTTTATGTCAATAAACGTTTCTTTGGATTATGTTCAACATTTTTTATGTCATATAATCTAAAACGTTGAATTAAGATCGGACGACACATACTCGAGTTAGTAAACCAAGTATTCCAAATACAAAGTCAGACTACGAACCAATTTTTCTCGATGAGATTATTCAAATTAGCATCTAAATTGGTTACTTTTTAAGTATGGATAAATTTTTATATGAATATGCATGCTCTTGCAGAAAAGTATATTTGAATACCAACATTTACTCTTTTGCTTCAACGTGCAGAAAACCACGAGAAAGCAAGCTTTTGGATGCTTCTCAGTTGTAACAAGCAGCAGTATCATTGAAGGGCTGTTGAAGAGAAAGTACTACCCTAGGCTGAAACTAGTCTAA

Protein sequence

>MS.gene37678.t1

MNEKERKKHIREPPSIPFIWETKPGIPIKDWKPVDSSFCQLPKTPLKQIASIPFVWEEKPGIPLPSFSHVDESETITSLDLESFSFDTNESFNSVPSLLANSLVSSTKLSTAMPLQQTPSSPASSEMDSSISSYETGRDIGYNEKLSSSLVELKGNDFENEDYTCDMSRKQPTLGELIMMSRRRSCIRKATQMNKWDPPKKTTRKQAFGCFSVVTSSSIIEGLLKRKYYPRLKLV