Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55013.t1 | XP_003624398.1 | 97.6 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 2.70E-60 | 241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55013.t1 | G7KV85 | 97.6 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 2.0e-60 | 241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene55013 | -0.80964 | 1.71E-50 | -1.69E-46 |
| MS.gene049281 | MS.gene55013 | 0.822274 | 2.59E-53 | -1.69E-46 |
| MS.gene049312 | MS.gene55013 | 0.814277 | 1.67E-51 | -1.69E-46 |
| MS.gene049611 | MS.gene55013 | 0.822864 | 1.89E-53 | -1.69E-46 |
| MS.gene050216 | MS.gene55013 | -0.81179 | 5.87E-51 | -1.69E-46 |
| MS.gene050363 | MS.gene55013 | 0.806819 | 6.84E-50 | -1.69E-46 |
| MS.gene050380 | MS.gene55013 | 0.847363 | 1.21E-59 | -1.69E-46 |
| MS.gene050381 | MS.gene55013 | 0.891964 | 2.45E-74 | -1.69E-46 |
| MS.gene050504 | MS.gene55013 | -0.820054 | 8.40E-53 | -1.69E-46 |
| MS.gene050868 | MS.gene55013 | -0.821111 | 4.80E-53 | -1.69E-46 |
| MS.gene050871 | MS.gene55013 | -0.821814 | 3.31E-53 | -1.69E-46 |
| MS.gene050883 | MS.gene55013 | 0.900612 | 6.15E-78 | -1.69E-46 |
| MS.gene05104 | MS.gene55013 | 0.830202 | 3.35E-55 | -1.69E-46 |
| MS.gene051127 | MS.gene55013 | 0.835582 | 1.54E-56 | -1.69E-46 |
| MS.gene051364 | MS.gene55013 | 0.81704 | 4.05E-52 | -1.69E-46 |
| MS.gene05141 | MS.gene55013 | 0.85515 | 7.65E-62 | -1.69E-46 |
| MS.gene051560 | MS.gene55013 | -0.801926 | 7.15E-49 | -1.69E-46 |
| MS.gene051694 | MS.gene55013 | -0.826477 | 2.66E-54 | -1.69E-46 |
| MS.gene051946 | MS.gene55013 | 0.875385 | 3.21E-68 | -1.69E-46 |
| MS.gene052164 | MS.gene55013 | -0.818055 | 2.39E-52 | -1.69E-46 |
| MS.gene052329 | MS.gene55013 | 0.809485 | 1.85E-50 | -1.69E-46 |
| MS.gene05234 | MS.gene55013 | 0.831567 | 1.55E-55 | -1.69E-46 |
| MS.gene052482 | MS.gene55013 | -0.826578 | 2.51E-54 | -1.69E-46 |
| MS.gene052486 | MS.gene55013 | -0.831109 | 2.01E-55 | -1.69E-46 |
| MS.gene053203 | MS.gene55013 | -0.8268 | 2.22E-54 | -1.69E-46 |
| MS.gene053420 | MS.gene55013 | 0.851417 | 9.00E-61 | -1.69E-46 |
| MS.gene053436 | MS.gene55013 | -0.822536 | 2.25E-53 | -1.69E-46 |
| MS.gene053549 | MS.gene55013 | 0.845631 | 3.60E-59 | -1.69E-46 |
| MS.gene053561 | MS.gene55013 | 0.810155 | 1.33E-50 | -1.69E-46 |
| MS.gene053626 | MS.gene55013 | -0.803735 | 3.03E-49 | -1.69E-46 |
| MS.gene053826 | MS.gene55013 | 0.848772 | 4.95E-60 | -1.69E-46 |
| MS.gene054003 | MS.gene55013 | -0.822685 | 2.08E-53 | -1.69E-46 |
| MS.gene054595 | MS.gene55013 | -0.825859 | 3.72E-54 | -1.69E-46 |
| MS.gene054707 | MS.gene55013 | 0.810382 | 1.18E-50 | -1.69E-46 |
| MS.gene054708 | MS.gene55013 | 0.850131 | 2.07E-60 | -1.69E-46 |
| MS.gene054959 | MS.gene55013 | 0.801071 | 1.07E-48 | -1.69E-46 |
| MS.gene055124 | MS.gene55013 | 0.826394 | 2.78E-54 | -1.69E-46 |
| MS.gene055139 | MS.gene55013 | 0.850686 | 1.45E-60 | -1.69E-46 |
| MS.gene055154 | MS.gene55013 | 0.843737 | 1.17E-58 | -1.69E-46 |
| MS.gene055202 | MS.gene55013 | 0.834452 | 2.97E-56 | -1.69E-46 |
| MS.gene055362 | MS.gene55013 | -0.805378 | 1.37E-49 | -1.69E-46 |
| MS.gene055502 | MS.gene55013 | -0.824421 | 8.15E-54 | -1.69E-46 |
| MS.gene055577 | MS.gene55013 | -0.809828 | 1.56E-50 | -1.69E-46 |
| MS.gene056310 | MS.gene55013 | 0.893579 | 5.51E-75 | -1.69E-46 |
| MS.gene056311 | MS.gene55013 | 0.906034 | 2.28E-80 | -1.69E-46 |
| MS.gene056313 | MS.gene55013 | 0.88924 | 2.89E-73 | -1.69E-46 |
| MS.gene056408 | MS.gene55013 | 0.829055 | 6.38E-55 | -1.69E-46 |
| MS.gene056504 | MS.gene55013 | -0.813923 | 2.00E-51 | -1.69E-46 |
| MS.gene056647 | MS.gene55013 | 0.807452 | 5.02E-50 | -1.69E-46 |
| MS.gene056878 | MS.gene55013 | -0.804105 | 2.53E-49 | -1.69E-46 |
| MS.gene057014 | MS.gene55013 | 0.872882 | 2.26E-67 | -1.69E-46 |
| MS.gene057173 | MS.gene55013 | 0.811151 | 8.08E-51 | -1.69E-46 |
| MS.gene057261 | MS.gene55013 | 0.800003 | 1.77E-48 | -1.69E-46 |
| MS.gene05726 | MS.gene55013 | 0.840395 | 8.93E-58 | -1.69E-46 |
| MS.gene057499 | MS.gene55013 | 0.817581 | 3.06E-52 | -1.69E-46 |
| MS.gene057506 | MS.gene55013 | 0.808361 | 3.22E-50 | -1.69E-46 |
| MS.gene058068 | MS.gene55013 | 0.81896 | 1.49E-52 | -1.69E-46 |
| MS.gene058099 | MS.gene55013 | 0.833981 | 3.90E-56 | -1.69E-46 |
| MS.gene058109 | MS.gene55013 | 0.83468 | 2.60E-56 | -1.69E-46 |
| MS.gene058111 | MS.gene55013 | 0.844136 | 9.11E-59 | -1.69E-46 |
| MS.gene058157 | MS.gene55013 | -0.86052 | 1.95E-63 | -1.69E-46 |
| MS.gene058170 | MS.gene55013 | 0.819297 | 1.25E-52 | -1.69E-46 |
| MS.gene058171 | MS.gene55013 | 0.847361 | 1.21E-59 | -1.69E-46 |
| MS.gene058173 | MS.gene55013 | 0.84263 | 2.30E-58 | -1.69E-46 |
| MS.gene058174 | MS.gene55013 | 0.816751 | 4.70E-52 | -1.69E-46 |
| MS.gene058175 | MS.gene55013 | 0.843904 | 1.05E-58 | -1.69E-46 |
| MS.gene05837 | MS.gene55013 | -0.82753 | 1.49E-54 | -1.69E-46 |
| MS.gene058493 | MS.gene55013 | 0.854671 | 1.05E-61 | -1.69E-46 |
| MS.gene058494 | MS.gene55013 | 0.85038 | 1.76E-60 | -1.69E-46 |
| MS.gene058630 | MS.gene55013 | 0.805345 | 1.40E-49 | -1.69E-46 |
| MS.gene059022 | MS.gene55013 | -0.813482 | 2.50E-51 | -1.69E-46 |
| MS.gene059052 | MS.gene55013 | 0.802934 | 4.43E-49 | -1.69E-46 |
| MS.gene059223 | MS.gene55013 | 0.853805 | 1.87E-61 | -1.69E-46 |
| MS.gene059236 | MS.gene55013 | 0.838498 | 2.78E-57 | -1.69E-46 |
| MS.gene059367 | MS.gene55013 | -0.800296 | 1.54E-48 | -1.69E-46 |
| MS.gene059718 | MS.gene55013 | -0.807422 | 5.09E-50 | -1.69E-46 |
| MS.gene059801 | MS.gene55013 | -0.801733 | 7.83E-49 | -1.69E-46 |
| MS.gene059865 | MS.gene55013 | -0.805176 | 1.51E-49 | -1.69E-46 |
| MS.gene060187 | MS.gene55013 | 0.835476 | 1.64E-56 | -1.69E-46 |
| MS.gene060482 | MS.gene55013 | -0.847001 | 1.52E-59 | -1.69E-46 |
| MS.gene060595 | MS.gene55013 | 0.837275 | 5.72E-57 | -1.69E-46 |
| MS.gene060778 | MS.gene55013 | -0.810969 | 8.85E-51 | -1.69E-46 |
| MS.gene06077 | MS.gene55013 | -0.844657 | 6.60E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55013.t1 | MTR_7g082760 | 97.600 | 125 | 3 | 0 | 1 | 125 | 2 | 126 | 6.16e-87 | 249 |
| MS.gene55013.t1 | MTR_7g082750 | 50.370 | 135 | 48 | 5 | 4 | 125 | 1 | 129 | 7.64e-36 | 119 |
| MS.gene55013.t1 | MTR_7g082740 | 52.756 | 127 | 45 | 4 | 14 | 125 | 4 | 130 | 6.06e-34 | 115 |
| MS.gene55013.t1 | MTR_7g082700 | 52.756 | 127 | 45 | 4 | 14 | 125 | 4 | 130 | 1.30e-33 | 114 |
| MS.gene55013.t1 | MTR_7g082730 | 55.200 | 125 | 41 | 5 | 15 | 125 | 5 | 128 | 4.50e-33 | 112 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 36 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCTTTGTCCACATCTTTAT+TGG | 0.171024 | 7.3:+25168587 | None:intergenic |
| CCGTAAGTACCTCGTTGGTT+TGG | 0.301020 | 7.3:+25168739 | None:intergenic |
| ACTTATATTTGAGATTTCTA+AGG | 0.310038 | 7.3:-25168450 | MS.gene55013:CDS |
| AGAAGGATTAAACTATTTCA+TGG | 0.325765 | 7.3:+25168400 | None:intergenic |
| CAAGGTTAACATGGGCTATT+AGG | 0.336227 | 7.3:-25168613 | MS.gene55013:CDS |
| ATGAAAAGTGATACCATCTT+TGG | 0.337936 | 7.3:-25168530 | MS.gene55013:CDS |
| CGTAAGTACCTCGTTGGTTT+GGG | 0.339937 | 7.3:+25168740 | None:intergenic |
| TAAGGATCGTGTAGTTTCTA+AGG | 0.360505 | 7.3:-25168504 | MS.gene55013:CDS |
| ATTTGAGATTTCTAAGGCCT+TGG | 0.387808 | 7.3:-25168444 | MS.gene55013:CDS |
| CATAATGAAAAGCGTGGAAA+TGG | 0.389387 | 7.3:-25168774 | None:intergenic |
| GAAGGAAGACCAAGGTTCTA+AGG | 0.400859 | 7.3:-25168670 | MS.gene55013:CDS |
| TGATACCATCTTTGGAACTA+AGG | 0.464934 | 7.3:-25168522 | MS.gene55013:CDS |
| TCTTCTCAACCTTAGAACCT+TGG | 0.471764 | 7.3:+25168661 | None:intergenic |
| AGGTTCTAAGGTTGAGAAGA+AGG | 0.473602 | 7.3:-25168658 | MS.gene55013:CDS |
| CCAAACCAACGAGGTACTTA+CGG | 0.478482 | 7.3:-25168739 | MS.gene55013:CDS |
| CTTCCTTCCATCACCGGTGA+TGG | 0.490534 | 7.3:+25168685 | None:intergenic |
| CGCCTCCGTAAGTACCTCGT+TGG | 0.498443 | 7.3:+25168734 | None:intergenic |
| CTTGGTCTTCCTTCCATCAC+CGG | 0.498548 | 7.3:+25168679 | None:intergenic |
| GAGTAGTTCCAAGGTTAACA+TGG | 0.518857 | 7.3:-25168622 | MS.gene55013:CDS |
| GAGGATGCCATCACCGGTGA+TGG | 0.521516 | 7.3:-25168692 | MS.gene55013:CDS |
| CTAATAGCCCATGTTAACCT+TGG | 0.534159 | 7.3:+25168614 | None:intergenic |
| CTCGACGAGGATGCCATCAC+CGG | 0.557338 | 7.3:-25168698 | MS.gene55013:CDS |
| TAAGGTTGAGAAGAAGGCAG+TGG | 0.557373 | 7.3:-25168652 | MS.gene55013:CDS |
| ACGATCCTTAGTTCCAAAGA+TGG | 0.563624 | 7.3:+25168517 | None:intergenic |
| ATGCCATCACCGGTGATGGA+AGG | 0.564522 | 7.3:-25168688 | MS.gene55013:CDS |
| GGTTGAGAAGAAGGCAGTGG+AGG | 0.585451 | 7.3:-25168649 | MS.gene55013:CDS |
| CTTCATCATAATGAAAAGCG+TGG | 0.588975 | 7.3:-25168780 | None:intergenic |
| GGAGGATAAGAGTAGTTCCA+AGG | 0.595694 | 7.3:-25168631 | MS.gene55013:CDS |
| CAACGAGGTACTTACGGAGG+CGG | 0.621368 | 7.3:-25168733 | MS.gene55013:CDS |
| AGTAGTTCCAAGGTTAACAT+GGG | 0.641526 | 7.3:-25168621 | MS.gene55013:CDS |
| TAATTCATGAGAAGCAACCA+AGG | 0.645785 | 7.3:+25168427 | None:intergenic |
| TTAGGTCACCAATAAAGATG+TGG | 0.652661 | 7.3:-25168595 | MS.gene55013:CDS |
| GCGATATCAGCGACTCGACG+AGG | 0.678608 | 7.3:-25168711 | MS.gene55013:CDS |
| CGGTGATGGAAGGAAGACCA+AGG | 0.711559 | 7.3:-25168678 | MS.gene55013:CDS |
| AACCAACGAGGTACTTACGG+AGG | 0.715584 | 7.3:-25168736 | MS.gene55013:CDS |
| CACTGATACCCAAACCAACG+AGG | 0.721556 | 7.3:-25168748 | MS.gene55013:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ACTTATATTTGAGATTTCTA+AGG | - | chr7.3:25168732-25168751 | MS.gene55013:CDS | 20.0% |
| ATGAAAAGTGATACCATCTT+TGG | - | chr7.3:25168652-25168671 | MS.gene55013:CDS | 30.0% | |
| !! | AAAGATGGTATCACTTTTCA+TGG | + | chr7.3:25168653-25168672 | None:intergenic | 30.0% |
| AGTAGTTCCAAGGTTAACAT+GGG | - | chr7.3:25168561-25168580 | MS.gene55013:CDS | 35.0% | |
| TAAGGATCGTGTAGTTTCTA+AGG | - | chr7.3:25168678-25168697 | MS.gene55013:CDS | 35.0% | |
| TAATTCATGAGAAGCAACCA+AGG | + | chr7.3:25168758-25168777 | None:intergenic | 35.0% | |
| TTAGGTCACCAATAAAGATG+TGG | - | chr7.3:25168587-25168606 | MS.gene55013:CDS | 35.0% | |
| ! | AGCTTTGTCCACATCTTTAT+TGG | + | chr7.3:25168598-25168617 | None:intergenic | 35.0% |
| ! | TTATTCTCGTGATGCTTTTG+AGG | - | chr7.3:25168705-25168724 | MS.gene55013:CDS | 35.0% |
| !! | ATTTGAGATTTCTAAGGCCT+TGG | - | chr7.3:25168738-25168757 | MS.gene55013:CDS | 35.0% |
| !! | TGATACCATCTTTGGAACTA+AGG | - | chr7.3:25168660-25168679 | MS.gene55013:CDS | 35.0% |
| ACGATCCTTAGTTCCAAAGA+TGG | + | chr7.3:25168668-25168687 | None:intergenic | 40.0% | |
| CTAATAGCCCATGTTAACCT+TGG | + | chr7.3:25168571-25168590 | None:intergenic | 40.0% | |
| GAGTAGTTCCAAGGTTAACA+TGG | - | chr7.3:25168560-25168579 | MS.gene55013:CDS | 40.0% | |
| TCTTCTCAACCTTAGAACCT+TGG | + | chr7.3:25168524-25168543 | None:intergenic | 40.0% | |
| ! | CAAGGTTAACATGGGCTATT+AGG | - | chr7.3:25168569-25168588 | MS.gene55013:CDS | 40.0% |
| !! | AGGTTCTAAGGTTGAGAAGA+AGG | - | chr7.3:25168524-25168543 | MS.gene55013:CDS | 40.0% |
| GAAGGAAGACCAAGGTTCTA+AGG | - | chr7.3:25168512-25168531 | MS.gene55013:CDS | 45.0% | |
| GGAGGATAAGAGTAGTTCCA+AGG | - | chr7.3:25168551-25168570 | MS.gene55013:CDS | 45.0% | |
| TAAGGTTGAGAAGAAGGCAG+TGG | - | chr7.3:25168530-25168549 | MS.gene55013:CDS | 45.0% | |
| ! | CCAAACCAACGAGGTACTTA+CGG | - | chr7.3:25168443-25168462 | MS.gene55013:CDS | 45.0% |
| !! | CGTAAGTACCTCGTTGGTTT+GGG | + | chr7.3:25168445-25168464 | None:intergenic | 45.0% |
| CACTGATACCCAAACCAACG+AGG | - | chr7.3:25168434-25168453 | MS.gene55013:CDS | 50.0% | |
| ! | AACCAACGAGGTACTTACGG+AGG | - | chr7.3:25168446-25168465 | MS.gene55013:CDS | 50.0% |
| ! | CCGTAAGTACCTCGTTGGTT+TGG | + | chr7.3:25168446-25168465 | None:intergenic | 50.0% |
| ! | CTTGGTCTTCCTTCCATCAC+CGG | + | chr7.3:25168506-25168525 | None:intergenic | 50.0% |
| CTTCCTTCCATCACCGGTGA+TGG | + | chr7.3:25168500-25168519 | None:intergenic | 55.0% | |
| GGTTGAGAAGAAGGCAGTGG+AGG | - | chr7.3:25168533-25168552 | MS.gene55013:CDS | 55.0% | |
| ! | CAACGAGGTACTTACGGAGG+CGG | - | chr7.3:25168449-25168468 | MS.gene55013:CDS | 55.0% |
| !! | ATGCCATCACCGGTGATGGA+AGG | - | chr7.3:25168494-25168513 | MS.gene55013:CDS | 55.0% |
| !! | CGGTGATGGAAGGAAGACCA+AGG | - | chr7.3:25168504-25168523 | MS.gene55013:CDS | 55.0% |
| CGCCTCCGTAAGTACCTCGT+TGG | + | chr7.3:25168451-25168470 | None:intergenic | 60.0% | |
| CTCGACGAGGATGCCATCAC+CGG | - | chr7.3:25168484-25168503 | MS.gene55013:CDS | 60.0% | |
| GAGGATGCCATCACCGGTGA+TGG | - | chr7.3:25168490-25168509 | MS.gene55013:CDS | 60.0% | |
| GCGATATCAGCGACTCGACG+AGG | - | chr7.3:25168471-25168490 | MS.gene55013:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 25168412 | 25168792 | 25168412 | ID=MS.gene55013 |
| chr7.3 | mRNA | 25168412 | 25168792 | 25168412 | ID=MS.gene55013.t1;Parent=MS.gene55013 |
| chr7.3 | exon | 25168412 | 25168792 | 25168412 | ID=MS.gene55013.t1.exon1;Parent=MS.gene55013.t1 |
| chr7.3 | CDS | 25168412 | 25168792 | 25168412 | ID=cds.MS.gene55013.t1;Parent=MS.gene55013.t1 |
| Gene Sequence |
| Protein sequence |
