AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene54303


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54303.t1 MTR_2g016030 96.894 161 4 1 1 160 1 161 4.60e-114 320
MS.gene54303.t1 MTR_6g012450 70.370 81 23 1 19 99 110 189 1.62e-36 131
MS.gene54303.t1 MTR_4g082060 67.500 80 26 0 20 99 96 175 1.73e-35 128
MS.gene54303.t1 MTR_7g010950 71.053 76 21 1 24 99 114 188 5.50e-35 127
MS.gene54303.t1 MTR_8g044220 61.628 86 32 1 25 109 96 181 1.26e-34 125
MS.gene54303.t1 MTR_7g086780 71.233 73 20 1 28 99 80 152 6.41e-33 120
MS.gene54303.t1 MTR_5g041380 58.065 93 37 1 18 108 97 189 3.84e-32 117
MS.gene54303.t1 MTR_6g027460 73.239 71 18 1 30 99 79 149 5.75e-32 117
MS.gene54303.t1 MTR_3g435480 57.778 90 29 1 19 99 100 189 6.51e-31 115
MS.gene54303.t1 MTR_5g041530 70.423 71 18 1 32 99 123 193 1.54e-30 114
MS.gene54303.t1 MTR_6g027450 69.014 71 21 1 30 99 72 142 1.60e-30 112
MS.gene54303.t1 MTR_5g041400 62.338 77 29 0 32 108 105 181 4.29e-30 112
MS.gene54303.t1 MTR_5g041420 71.667 60 17 0 40 99 132 191 8.20e-30 110
MS.gene54303.t1 MTR_2g096740 55.263 76 34 0 24 99 40 115 2.09e-27 103
MS.gene54303.t1 MTR_2g096740 55.263 76 34 0 24 99 55 130 2.30e-27 103
MS.gene54303.t1 MTR_8g015840 59.155 71 23 1 34 98 2 72 3.92e-26 99.0
MS.gene54303.t1 MTR_8g068210 47.059 102 48 3 24 123 12 109 4.79e-26 101
MS.gene54303.t1 MTR_8g479350 48.000 100 41 2 48 138 70 167 9.76e-26 100
MS.gene54303.t1 MTR_4g022370 70.175 57 17 0 43 99 79 135 1.30e-25 100
MS.gene54303.t1 MTR_8g027295 56.098 82 32 2 36 113 12 93 3.53e-25 97.8
MS.gene54303.t1 MTR_8g079060 71.154 52 15 0 46 97 29 80 4.16e-25 96.7
MS.gene54303.t1 MTR_4g063780 64.286 70 24 1 43 111 72 141 1.23e-24 97.4
MS.gene54303.t1 MTR_2g093220 55.263 76 34 0 24 99 18 93 1.66e-24 96.3
MS.gene54303.t1 MTR_4g109980 69.811 53 16 0 44 96 38 90 1.70e-24 96.7
MS.gene54303.t1 MTR_3g091820 64.912 57 20 0 43 99 40 96 1.95e-24 96.3
MS.gene54303.t1 MTR_2g059540 73.469 49 13 0 46 94 31 79 2.75e-24 94.0
MS.gene54303.t1 MTR_7g024670 76.000 50 12 0 48 97 79 128 4.21e-24 96.7
MS.gene54303.t1 MTR_7g059400 68.421 57 18 0 43 99 73 129 1.01e-23 95.1
MS.gene54303.t1 MTR_1g077600 54.412 68 31 0 30 97 27 94 1.14e-23 93.6
MS.gene54303.t1 MTR_1g077600 54.412 68 31 0 30 97 27 94 1.36e-23 94.0
MS.gene54303.t1 MTR_4g088580 73.077 52 14 0 48 99 57 108 1.91e-23 94.7
MS.gene54303.t1 MTR_5g031440 68.519 54 17 0 43 96 40 93 1.97e-23 94.4
MS.gene54303.t1 MTR_4g089095 67.308 52 17 0 48 99 52 103 3.48e-23 92.8
MS.gene54303.t1 MTR_4g089095 67.308 52 17 0 48 99 18 69 4.70e-23 92.0
MS.gene54303.t1 MTR_4g461080 54.054 74 34 0 24 97 7 80 9.08e-23 91.7
MS.gene54303.t1 MTR_2g014060 52.632 76 29 1 30 98 2 77 1.11e-22 92.0
MS.gene54303.t1 MTR_1g056810 64.516 62 21 1 39 99 4 65 1.16e-22 91.3
MS.gene54303.t1 MTR_3g090430 65.517 58 20 0 42 99 47 104 1.29e-22 92.0
MS.gene54303.t1 MTR_2g014170 71.429 49 14 0 46 94 45 93 1.51e-22 91.3
MS.gene54303.t1 MTR_2g013370 41.489 94 50 1 6 99 11 99 2.11e-22 90.5
MS.gene54303.t1 MTR_3g077750 66.667 51 17 0 46 96 61 111 1.49e-21 89.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene54303.t1 AT1G29160 62.281 114 23 1 8 101 4 117 3.97e-46 149
MS.gene54303.t1 AT2G34140 63.636 110 24 1 8 101 4 113 6.40e-45 145
MS.gene54303.t1 AT5G62430 58.416 101 28 2 12 99 8 107 1.96e-35 125
MS.gene54303.t1 AT3G47500 58.252 103 43 0 20 122 84 186 4.22e-35 127
MS.gene54303.t1 AT5G39660 82.540 63 11 0 40 102 132 194 1.58e-34 125
MS.gene54303.t1 AT5G39660 82.540 63 11 0 40 102 132 194 1.58e-34 125
MS.gene54303.t1 AT5G39660 82.540 63 11 0 40 102 132 194 1.58e-34 125
MS.gene54303.t1 AT1G26790 56.311 103 38 1 23 125 88 183 3.43e-34 123
MS.gene54303.t1 AT1G69570 60.440 91 29 1 34 124 120 203 5.00e-33 120
MS.gene54303.t1 AT5G60850 57.692 78 33 0 46 123 51 128 2.69e-27 103
MS.gene54303.t1 AT1G51700 71.698 53 15 0 46 98 33 85 3.77e-26 98.6
MS.gene54303.t1 AT3G21270 76.923 52 12 0 46 97 29 80 8.52e-26 97.8
MS.gene54303.t1 AT2G28810 72.727 55 15 0 43 97 74 128 1.63e-25 99.8
MS.gene54303.t1 AT3G50410 71.698 53 15 0 46 98 30 82 2.09e-25 97.8
MS.gene54303.t1 AT4G38000 60.294 68 26 1 43 109 38 105 2.12e-25 97.8
MS.gene54303.t1 AT2G28810 72.727 55 15 0 43 97 91 145 2.63e-25 99.4
MS.gene54303.t1 AT2G37590 68.421 57 18 0 43 99 86 142 7.64e-25 97.8
MS.gene54303.t1 AT3G55370 68.421 57 18 0 43 99 73 129 7.84e-25 97.8
MS.gene54303.t1 AT3G55370 68.421 57 18 0 43 99 73 129 8.04e-25 98.2
MS.gene54303.t1 AT5G65590 68.421 57 18 0 43 99 38 94 1.16e-24 97.4
MS.gene54303.t1 AT3G55370 68.421 57 18 0 43 99 118 174 1.16e-24 98.2
MS.gene54303.t1 AT1G07640 52.500 80 38 0 18 97 49 128 2.69e-24 96.7
MS.gene54303.t1 AT1G07640 76.000 50 12 0 48 97 23 72 3.07e-24 95.5
MS.gene54303.t1 AT1G07640 52.500 80 38 0 18 97 57 136 3.30e-24 96.3
MS.gene54303.t1 AT5G66940 67.925 53 17 0 46 98 32 84 7.88e-24 93.2
MS.gene54303.t1 AT5G60200 66.667 57 19 0 43 99 52 108 1.01e-23 93.6
MS.gene54303.t1 AT5G02460 68.421 57 18 0 43 99 92 148 1.65e-23 95.1
MS.gene54303.t1 AT3G45610 52.778 72 34 0 28 99 22 93 1.93e-23 92.8
MS.gene54303.t1 AT4G24060 63.158 57 21 0 43 99 50 106 2.04e-23 94.4
MS.gene54303.t1 AT1G47655 64.815 54 19 0 41 94 24 77 3.05e-23 91.3
MS.gene54303.t1 AT3G52440 66.102 59 19 1 44 101 22 80 3.55e-23 92.0
MS.gene54303.t1 AT1G28310 68.519 54 17 0 46 99 27 80 3.69e-23 93.2
MS.gene54303.t1 AT3G52440 66.102 59 19 1 44 101 41 99 3.92e-23 92.4
MS.gene54303.t1 AT3G61850 57.812 64 27 0 36 99 52 115 3.98e-23 92.4
MS.gene54303.t1 AT3G61850 57.812 64 27 0 36 99 52 115 3.98e-23 92.4
MS.gene54303.t1 AT3G61850 57.812 64 27 0 36 99 64 127 4.45e-23 92.8
MS.gene54303.t1 AT1G28310 68.519 54 17 0 46 99 51 104 5.88e-23 92.8
MS.gene54303.t1 AT3G61850 57.812 64 27 0 36 99 52 115 6.03e-23 92.4
MS.gene54303.t1 AT3G61850 57.812 64 27 0 36 99 64 127 7.29e-23 92.4
MS.gene54303.t1 AT1G64620 65.385 52 18 0 43 94 46 97 9.91e-23 92.4
MS.gene54303.t1 AT2G28510 71.154 52 15 0 48 99 50 101 1.03e-22 91.7
MS.gene54303.t1 AT4G00940 71.154 52 15 0 48 99 70 121 1.57e-22 91.3
MS.gene54303.t1 AT4G00940 71.154 52 15 0 48 99 70 121 1.57e-22 91.3
MS.gene54303.t1 AT4G00940 71.154 52 15 0 48 99 70 121 1.57e-22 91.3
MS.gene54303.t1 AT1G21340 70.000 50 15 0 48 97 40 89 2.02e-22 90.1
MS.gene54303.t1 AT5G62940 69.388 49 15 0 48 96 75 123 1.15e-21 90.1
MS.gene54303.t1 AT4G21050 58.730 63 26 0 48 110 26 88 1.95e-21 86.7
MS.gene54303.t1 AT2G46590 68.750 48 15 0 46 93 68 115 5.17e-21 87.8
MS.gene54303.t1 AT2G46590 68.750 48 15 0 46 93 80 127 6.02e-21 87.8
MS.gene54303.t1 AT4G21040 60.000 60 20 1 48 103 27 86 2.22e-19 81.6
MS.gene54303.t1 AT4G21080 54.412 68 27 1 48 111 27 94 8.99e-19 80.5
MS.gene54303.t1 AT4G21030 45.588 68 37 0 48 115 23 90 2.10e-14 67.8

Find 45 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATACAAGGGATTAAGCTATT+TGG 0.296201 2.3:+69405602 MS.gene54303:CDS
GGTCTTCCTCCGCCGCTTGC+CGG 0.325640 2.3:-69406002 None:intergenic
GCTTCATAGACACAACTTTC+CGG 0.327262 2.3:-69405899 None:intergenic
GTAAGAGCTGTCAAAGATAC+TGG 0.350596 2.3:+69405792 MS.gene54303:CDS
ATGTCCAAGATGCAAGAGTA+TGG 0.357770 2.3:+69405715 MS.gene54303:CDS
GATGATGGCCATAACTACTT+AGG 0.390058 2.3:+69405932 MS.gene54303:CDS
GTTCTAACCCTAAGTAGTTA+TGG 0.403963 2.3:-69405940 None:intergenic
GTTTCCATACTCTTGCATCT+TGG 0.414049 2.3:-69405719 None:intergenic
TGGGGCCCTCCGTAACGTTC+CGG 0.432988 2.3:+69405823 MS.gene54303:CDS
TTCCGACGACCTGCTCCAAC+CGG 0.436863 2.3:-69405842 None:intergenic
ATCCTCAAGACTTGGCGGCT+TGG 0.465996 2.3:-69405867 None:intergenic
CGTAACGTTCCGGTTGGAGC+AGG 0.491556 2.3:+69405833 MS.gene54303:CDS
CAAAGATACTGGACGGCTGG+TGG 0.496439 2.3:+69405803 MS.gene54303:CDS
ATGATGGCCATAACTACTTA+GGG 0.502994 2.3:+69405933 MS.gene54303:CDS
CTGCTCCAACCGGAACGTTA+CGG 0.518351 2.3:-69405832 None:intergenic
AAAAGAAAGTGAAGATCAAA+CGG 0.525439 2.3:+69405664 MS.gene54303:CDS
GCCCTCCGTAACGTTCCGGT+TGG 0.526190 2.3:+69405827 MS.gene54303:CDS
AAAGATACTGGACGGCTGGT+GGG 0.536772 2.3:+69405804 MS.gene54303:CDS
TTGCATGAGGAATTAAAAGA+AGG 0.537463 2.3:+69405638 MS.gene54303:CDS
TCTTGAGGATAGCGGTTCAC+CGG 0.541063 2.3:+69405880 MS.gene54303:CDS
TGTCAAAGATACTGGACGGC+TGG 0.542444 2.3:+69405800 MS.gene54303:CDS
AGAAAAGGTGAAGAGATACA+AGG 0.544735 2.3:+69405587 MS.gene54303:CDS
ATACTCTTGCATCTTGGACA+TGG 0.546078 2.3:-69405713 None:intergenic
GGCCAAGCCGCCAAGTCTTG+AGG 0.555303 2.3:+69405865 MS.gene54303:CDS
TTCCGGTTGGAGCAGGTCGT+CGG 0.558411 2.3:+69405840 MS.gene54303:CDS
AAGCGGCGGAGGAAGACCTC+AGG 0.572311 2.3:+69406007 MS.gene54303:CDS
AAAATGGCTGAAGTGAGAAA+AGG 0.579551 2.3:+69405572 None:intergenic
AAGATACTGGACGGCTGGTG+GGG 0.581078 2.3:+69405805 MS.gene54303:CDS
CCGCTATCCTCAAGACTTGG+CGG 0.597183 2.3:-69405872 None:intergenic
GAACCGCTATCCTCAAGACT+TGG 0.599554 2.3:-69405875 None:intergenic
GAAAAGGTGAAGAGATACAA+GGG 0.603099 2.3:+69405588 MS.gene54303:CDS
GGTTGGAGCAGGTCGTCGGA+AGG 0.608139 2.3:+69405844 MS.gene54303:CDS
CACAACAATAAAATTGCATG+AGG 0.620119 2.3:+69405625 MS.gene54303:CDS
GAAGCTGCTAGTTCTGATGA+TGG 0.628789 2.3:+69405917 MS.gene54303:CDS
CGGCGGAGGAAGACCTCAGG+TGG 0.630380 2.3:+69406010 MS.gene54303:CDS
TGCTAATGAATAACCACCTG+AGG 0.631649 2.3:-69406023 None:intergenic
GAGCTGTCAAAGATACTGGA+CGG 0.644495 2.3:+69405796 MS.gene54303:CDS
TCCAACCGGAACGTTACGGA+GGG 0.647718 2.3:-69405828 None:intergenic
TCCCGAAAATCACTCTGAGG+AGG 0.648141 2.3:-69405971 None:intergenic
CTCCAACCGGAACGTTACGG+AGG 0.649811 2.3:-69405829 None:intergenic
ACTTCCCGAAAATCACTCTG+AGG 0.686816 2.3:-69405974 None:intergenic
GTAAAGAACAAGAAATCACA+AGG 0.691544 2.3:-69406050 None:intergenic
AGAAAGTGAAGATCAAACGG+TGG 0.696077 2.3:+69405667 MS.gene54303:CDS
CCGCCAAGTCTTGAGGATAG+CGG 0.704408 2.3:+69405872 MS.gene54303:CDS
AAGAACAAGAAATCACAAGG+AGG 0.761754 2.3:-69406047 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAAGAAAGTGAAGATCAAA+CGG + chr2.3:69405664-69405683 MS.gene54303:CDS 25.0%
ATACAAGGGATTAAGCTATT+TGG + chr2.3:69405602-69405621 MS.gene54303:CDS 30.0%
CACAACAATAAAATTGCATG+AGG + chr2.3:69405625-69405644 MS.gene54303:CDS 30.0%
TTGCATGAGGAATTAAAAGA+AGG + chr2.3:69405638-69405657 MS.gene54303:CDS 30.0%
AGAAAAGGTGAAGAGATACA+AGG + chr2.3:69405587-69405606 MS.gene54303:CDS 35.0%
ATGATGGCCATAACTACTTA+GGG + chr2.3:69405933-69405952 MS.gene54303:CDS 35.0%
CAGCTCTTACAAAAATGTCT+TGG - chr2.3:69405782-69405801 None:intergenic 35.0%
GAAAAGGTGAAGAGATACAA+GGG + chr2.3:69405588-69405607 MS.gene54303:CDS 35.0%
GTTCTAACCCTAAGTAGTTA+TGG - chr2.3:69405943-69405962 None:intergenic 35.0%
!!! GATTTTCGGGAAGTATTTTC+CGG + chr2.3:69405983-69406002 MS.gene54303:CDS 35.0%
AGAAAGTGAAGATCAAACGG+TGG + chr2.3:69405667-69405686 MS.gene54303:CDS 40.0%
ATACTCTTGCATCTTGGACA+TGG - chr2.3:69405716-69405735 None:intergenic 40.0%
ATGTCCAAGATGCAAGAGTA+TGG + chr2.3:69405715-69405734 MS.gene54303:CDS 40.0%
GCTTCATAGACACAACTTTC+CGG - chr2.3:69405902-69405921 None:intergenic 40.0%
GTAAGAGCTGTCAAAGATAC+TGG + chr2.3:69405792-69405811 MS.gene54303:CDS 40.0%
GTTTCCATACTCTTGCATCT+TGG - chr2.3:69405722-69405741 None:intergenic 40.0%
TGCTAATGAATAACCACCTG+AGG - chr2.3:69406026-69406045 None:intergenic 40.0%
! GATGATGGCCATAACTACTT+AGG + chr2.3:69405932-69405951 MS.gene54303:CDS 40.0%
ACTTCCCGAAAATCACTCTG+AGG - chr2.3:69405977-69405996 None:intergenic 45.0%
GAGCTGTCAAAGATACTGGA+CGG + chr2.3:69405796-69405815 MS.gene54303:CDS 45.0%
! TGCCTCCTCAGAGTGATTTT+CGG + chr2.3:69405969-69405988 MS.gene54303:CDS 45.0%
!! GAAGCTGCTAGTTCTGATGA+TGG + chr2.3:69405917-69405936 MS.gene54303:CDS 45.0%
AAAGATACTGGACGGCTGGT+GGG + chr2.3:69405804-69405823 MS.gene54303:CDS 50.0%
GAACCGCTATCCTCAAGACT+TGG - chr2.3:69405878-69405897 None:intergenic 50.0%
TGTCAAAGATACTGGACGGC+TGG + chr2.3:69405800-69405819 MS.gene54303:CDS 50.0%
! AAGTATTTTCCGGCAAGCGG+CGG + chr2.3:69405993-69406012 MS.gene54303:CDS 50.0%
! GCCTCCTCAGAGTGATTTTC+GGG + chr2.3:69405970-69405989 MS.gene54303:CDS 50.0%
! TCCCGAAAATCACTCTGAGG+AGG - chr2.3:69405974-69405993 None:intergenic 50.0%
! TCTTGAGGATAGCGGTTCAC+CGG + chr2.3:69405880-69405899 MS.gene54303:CDS 50.0%
!!! GGGAAGTATTTTCCGGCAAG+CGG + chr2.3:69405990-69406009 MS.gene54303:CDS 50.0%
AAGATACTGGACGGCTGGTG+GGG + chr2.3:69405805-69405824 MS.gene54303:CDS 55.0%
ATCCTCAAGACTTGGCGGCT+TGG - chr2.3:69405870-69405889 None:intergenic 55.0%
CAAAGATACTGGACGGCTGG+TGG + chr2.3:69405803-69405822 MS.gene54303:CDS 55.0%
CCGCCAAGTCTTGAGGATAG+CGG + chr2.3:69405872-69405891 MS.gene54303:CDS 55.0%
CCGCTATCCTCAAGACTTGG+CGG - chr2.3:69405875-69405894 None:intergenic 55.0%
CTGCTCCAACCGGAACGTTA+CGG - chr2.3:69405835-69405854 None:intergenic 55.0%
TCCAACCGGAACGTTACGGA+GGG - chr2.3:69405831-69405850 None:intergenic 55.0%
CGTAACGTTCCGGTTGGAGC+AGG + chr2.3:69405833-69405852 MS.gene54303:CDS 60.0%
CTCCAACCGGAACGTTACGG+AGG - chr2.3:69405832-69405851 None:intergenic 60.0%
TTCCGACGACCTGCTCCAAC+CGG - chr2.3:69405845-69405864 None:intergenic 60.0%
TTCCGGTTGGAGCAGGTCGT+CGG + chr2.3:69405840-69405859 MS.gene54303:CDS 60.0%
! TATTTTCCGGCAAGCGGCGG+AGG + chr2.3:69405996-69406015 MS.gene54303:CDS 60.0%
AAGCGGCGGAGGAAGACCTC+AGG + chr2.3:69406007-69406026 MS.gene54303:CDS 65.0%
GCCCTCCGTAACGTTCCGGT+TGG + chr2.3:69405827-69405846 MS.gene54303:CDS 65.0%
GGCCAAGCCGCCAAGTCTTG+AGG + chr2.3:69405865-69405884 MS.gene54303:CDS 65.0%
GGTTGGAGCAGGTCGTCGGA+AGG + chr2.3:69405844-69405863 MS.gene54303:CDS 65.0%
TGGGGCCCTCCGTAACGTTC+CGG + chr2.3:69405823-69405842 MS.gene54303:CDS 65.0%
CGGCGGAGGAAGACCTCAGG+TGG + chr2.3:69406010-69406029 MS.gene54303:CDS 70.0%
GGTCTTCCTCCGCCGCTTGC+CGG - chr2.3:69406005-69406024 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 69405575 69406057 69405575 ID=MS.gene54303
chr2.3 mRNA 69405575 69406057 69405575 ID=MS.gene54303.t1;Parent=MS.gene54303
chr2.3 exon 69405575 69406057 69405575 ID=MS.gene54303.t1.exon1;Parent=MS.gene54303.t1
chr2.3 CDS 69405575 69406057 69405575 ID=cds.MS.gene54303.t1;Parent=MS.gene54303.t1
Gene Sequence

>MS.gene54303

ATGGCTGAAGTGAGAAAAGGTGAAGAGATACAAGGGATTAAGCTATTTGGCACAACAATAAAATTGCATGAGGAATTAAAAGAAGGTGAAAAAGAAAGTGAAGATCAAACGGTGGAGAAGAAGATAGAAAAGATCATACCATGTCCAAGATGCAAGAGTATGGAAACTAAATTTTGTTATTTCAATAATTATAACGTTAATCAACCAAGACATTTTTGTAAGAGCTGTCAAAGATACTGGACGGCTGGTGGGGCCCTCCGTAACGTTCCGGTTGGAGCAGGTCGTCGGAAGGCCAAGCCGCCAAGTCTTGAGGATAGCGGTTCACCGGAAAGTTGTGTCTATGAAGCTGCTAGTTCTGATGATGGCCATAACTACTTAGGGTTAGAACAATTTATGCCTCCTCAGAGTGATTTTCGGGAAGTATTTTCCGGCAAGCGGCGGAGGAAGACCTCAGGTGGTTATTCATTAGCATCCTCCTTGTGA

Protein sequence

>MS.gene54303.t1

MAEVRKGEEIQGIKLFGTTIKLHEELKEGEKESEDQTVEKKIEKIIPCPRCKSMETKFCYFNNYNVNQPRHFCKSCQRYWTAGGALRNVPVGAGRRKAKPPSLEDSGSPESCVYEAASSDDGHNYLGLEQFMPPQSDFREVFSGKRRRKTSGGYSLASSL