Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84043.t1 | XP_003590247.2 | 98.5 | 204 | 3 | 0 | 1 | 204 | 1 | 204 | 3.30E-103 | 384.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84043.t1 | G7I7C8 | 98.5 | 204 | 3 | 0 | 1 | 204 | 1 | 204 | 2.4e-103 | 384.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049171 | MS.gene84043 | 0.874499 | 6.44E-68 | -1.69E-46 |
| MS.gene049173 | MS.gene84043 | 0.826656 | 2.41E-54 | -1.69E-46 |
| MS.gene049217 | MS.gene84043 | 0.867609 | 1.21E-65 | -1.69E-46 |
| MS.gene049222 | MS.gene84043 | 0.885178 | 1.02E-71 | -1.69E-46 |
| MS.gene049232 | MS.gene84043 | 0.886367 | 3.64E-72 | -1.69E-46 |
| MS.gene049285 | MS.gene84043 | 0.906591 | 1.26E-80 | -1.69E-46 |
| MS.gene049313 | MS.gene84043 | -0.803123 | 4.05E-49 | -1.69E-46 |
| MS.gene049320 | MS.gene84043 | 0.879616 | 1.08E-69 | -1.69E-46 |
| MS.gene04939 | MS.gene84043 | 0.824181 | 9.28E-54 | -1.69E-46 |
| MS.gene049600 | MS.gene84043 | 0.817469 | 3.24E-52 | -1.69E-46 |
| MS.gene049609 | MS.gene84043 | 0.818055 | 2.39E-52 | -1.69E-46 |
| MS.gene049627 | MS.gene84043 | 0.873632 | 1.26E-67 | -1.69E-46 |
| MS.gene049633 | MS.gene84043 | 0.846253 | 2.44E-59 | -1.69E-46 |
| MS.gene049657 | MS.gene84043 | 0.812162 | 4.87E-51 | -1.69E-46 |
| MS.gene049865 | MS.gene84043 | 0.835375 | 1.74E-56 | -1.69E-46 |
| MS.gene05010 | MS.gene84043 | 0.880084 | 7.35E-70 | -1.69E-46 |
| MS.gene050163 | MS.gene84043 | 0.88964 | 2.02E-73 | -1.69E-46 |
| MS.gene050165 | MS.gene84043 | 0.898158 | 6.98E-77 | -1.69E-46 |
| MS.gene050170 | MS.gene84043 | 0.861691 | 8.58E-64 | -1.69E-46 |
| MS.gene050216 | MS.gene84043 | 0.835114 | 2.03E-56 | -1.69E-46 |
| MS.gene050323 | MS.gene84043 | 0.866096 | 3.66E-65 | -1.69E-46 |
| MS.gene050333 | MS.gene84043 | 0.891614 | 3.38E-74 | -1.69E-46 |
| MS.gene050381 | MS.gene84043 | -0.808401 | 3.15E-50 | -1.69E-46 |
| MS.gene050444 | MS.gene84043 | 0.821277 | 4.40E-53 | -1.69E-46 |
| MS.gene050496 | MS.gene84043 | 0.881047 | 3.32E-70 | -1.69E-46 |
| MS.gene050504 | MS.gene84043 | 0.909931 | 3.30E-82 | -1.69E-46 |
| MS.gene050518 | MS.gene84043 | 0.879805 | 9.23E-70 | -1.69E-46 |
| MS.gene050577 | MS.gene84043 | 0.871151 | 8.50E-67 | -1.69E-46 |
| MS.gene050612 | MS.gene84043 | 0.89068 | 7.91E-74 | -1.69E-46 |
| MS.gene050675 | MS.gene84043 | 0.883218 | 5.40E-71 | -1.69E-46 |
| MS.gene050699 | MS.gene84043 | 0.87038 | 1.52E-66 | -1.69E-46 |
| MS.gene050747 | MS.gene84043 | 0.829959 | 3.84E-55 | -1.69E-46 |
| MS.gene050772 | MS.gene84043 | 0.85335 | 2.53E-61 | -1.69E-46 |
| MS.gene05080 | MS.gene84043 | 0.826686 | 2.37E-54 | -1.69E-46 |
| MS.gene050868 | MS.gene84043 | 0.863889 | 1.80E-64 | -1.69E-46 |
| MS.gene050871 | MS.gene84043 | 0.863443 | 2.48E-64 | -1.69E-46 |
| MS.gene050876 | MS.gene84043 | 0.912346 | 2.17E-83 | -1.69E-46 |
| MS.gene05087 | MS.gene84043 | 0.864868 | 8.92E-65 | -1.69E-46 |
| MS.gene050883 | MS.gene84043 | -0.802566 | 5.28E-49 | -1.69E-46 |
| MS.gene050904 | MS.gene84043 | 0.90413 | 1.69E-79 | -1.69E-46 |
| MS.gene050950 | MS.gene84043 | 0.822802 | 1.95E-53 | -1.69E-46 |
| MS.gene050986 | MS.gene84043 | 0.898619 | 4.44E-77 | -1.69E-46 |
| MS.gene05100 | MS.gene84043 | 0.846291 | 2.38E-59 | -1.69E-46 |
| MS.gene051127 | MS.gene84043 | -0.802027 | 6.82E-49 | -1.69E-46 |
| MS.gene051268 | MS.gene84043 | 0.855173 | 7.53E-62 | -1.69E-46 |
| MS.gene051317 | MS.gene84043 | 0.847366 | 1.21E-59 | -1.69E-46 |
| MS.gene051336 | MS.gene84043 | 0.80222 | 6.22E-49 | -1.69E-46 |
| MS.gene051364 | MS.gene84043 | -0.802793 | 4.74E-49 | -1.69E-46 |
| MS.gene051509 | MS.gene84043 | 0.813932 | 1.99E-51 | -1.69E-46 |
| MS.gene051551 | MS.gene84043 | 0.845905 | 3.03E-59 | -1.69E-46 |
| MS.gene051564 | MS.gene84043 | 0.824446 | 8.04E-54 | -1.69E-46 |
| MS.gene051694 | MS.gene84043 | 0.81986 | 9.30E-53 | -1.69E-46 |
| MS.gene051810 | MS.gene84043 | 0.841798 | 3.82E-58 | -1.69E-46 |
| MS.gene051858 | MS.gene84043 | 0.820082 | 8.28E-53 | -1.69E-46 |
| MS.gene051946 | MS.gene84043 | -0.809208 | 2.12E-50 | -1.69E-46 |
| MS.gene051949 | MS.gene84043 | 0.818161 | 2.26E-52 | -1.69E-46 |
| MS.gene052164 | MS.gene84043 | 0.872171 | 3.90E-67 | -1.69E-46 |
| MS.gene052271 | MS.gene84043 | 0.812992 | 3.21E-51 | -1.69E-46 |
| MS.gene052482 | MS.gene84043 | 0.907188 | 6.64E-81 | -1.69E-46 |
| MS.gene052486 | MS.gene84043 | 0.920398 | 1.35E-87 | -1.69E-46 |
| MS.gene052503 | MS.gene84043 | 0.828551 | 8.44E-55 | -1.69E-46 |
| MS.gene052615 | MS.gene84043 | 0.812426 | 4.26E-51 | -1.69E-46 |
| MS.gene052670 | MS.gene84043 | 0.816974 | 4.19E-52 | -1.69E-46 |
| MS.gene052822 | MS.gene84043 | 0.807313 | 5.37E-50 | -1.69E-46 |
| MS.gene052823 | MS.gene84043 | 0.825485 | 4.57E-54 | -1.69E-46 |
| MS.gene052825 | MS.gene84043 | 0.8185 | 1.90E-52 | -1.69E-46 |
| MS.gene052916 | MS.gene84043 | 0.817133 | 3.86E-52 | -1.69E-46 |
| MS.gene052966 | MS.gene84043 | 0.868885 | 4.68E-66 | -1.69E-46 |
| MS.gene053083 | MS.gene84043 | -0.82623 | 3.04E-54 | -1.69E-46 |
| MS.gene053203 | MS.gene84043 | 0.840698 | 7.44E-58 | -1.69E-46 |
| MS.gene053213 | MS.gene84043 | 0.855977 | 4.39E-62 | -1.69E-46 |
| MS.gene053286 | MS.gene84043 | 0.85951 | 3.93E-63 | -1.69E-46 |
| MS.gene053420 | MS.gene84043 | -0.844599 | 6.84E-59 | -1.69E-46 |
| MS.gene053436 | MS.gene84043 | 0.818051 | 2.40E-52 | -1.69E-46 |
| MS.gene053479 | MS.gene84043 | 0.81297 | 3.24E-51 | -1.69E-46 |
| MS.gene053480 | MS.gene84043 | 0.842689 | 2.22E-58 | -1.69E-46 |
| MS.gene053549 | MS.gene84043 | -0.830226 | 3.31E-55 | -1.69E-46 |
| MS.gene053574 | MS.gene84043 | 0.849479 | 3.15E-60 | -1.69E-46 |
| MS.gene053626 | MS.gene84043 | 0.833592 | 4.88E-56 | -1.69E-46 |
| MS.gene053632 | MS.gene84043 | 0.835063 | 2.09E-56 | -1.69E-46 |
| MS.gene053633 | MS.gene84043 | 0.829531 | 4.89E-55 | -1.69E-46 |
| MS.gene053824 | MS.gene84043 | 0.860158 | 2.51E-63 | -1.69E-46 |
| MS.gene053916 | MS.gene84043 | 0.804244 | 2.37E-49 | -1.69E-46 |
| MS.gene054408 | MS.gene84043 | 0.822501 | 2.29E-53 | -1.69E-46 |
| MS.gene054595 | MS.gene84043 | 0.853046 | 3.09E-61 | -1.69E-46 |
| MS.gene054600 | MS.gene84043 | 0.858315 | 8.96E-63 | -1.69E-46 |
| MS.gene054616 | MS.gene84043 | -0.828908 | 6.92E-55 | -1.69E-46 |
| MS.gene054638 | MS.gene84043 | 0.807763 | 4.31E-50 | -1.69E-46 |
| MS.gene054642 | MS.gene84043 | 0.861898 | 7.42E-64 | -1.69E-46 |
| MS.gene05470 | MS.gene84043 | 0.86638 | 2.97E-65 | -1.69E-46 |
| MS.gene054954 | MS.gene84043 | 0.912276 | 2.35E-83 | -1.69E-46 |
| MS.gene055154 | MS.gene84043 | -0.813608 | 2.35E-51 | -1.69E-46 |
| MS.gene055333 | MS.gene84043 | -0.816815 | 4.55E-52 | -1.69E-46 |
| MS.gene055362 | MS.gene84043 | 0.89462 | 2.08E-75 | -1.69E-46 |
| MS.gene055502 | MS.gene84043 | 0.887065 | 1.98E-72 | -1.69E-46 |
| MS.gene055577 | MS.gene84043 | 0.874148 | 8.47E-68 | -1.69E-46 |
| MS.gene055634 | MS.gene84043 | 0.862824 | 3.85E-64 | -1.69E-46 |
| MS.gene055636 | MS.gene84043 | 0.805851 | 1.09E-49 | -1.69E-46 |
| MS.gene055638 | MS.gene84043 | 0.84313 | 1.69E-58 | -1.69E-46 |
| MS.gene055722 | MS.gene84043 | 0.816962 | 4.22E-52 | -1.69E-46 |
| MS.gene055748 | MS.gene84043 | 0.862171 | 6.12E-64 | -1.69E-46 |
| MS.gene055750 | MS.gene84043 | 0.882041 | 1.45E-70 | -1.69E-46 |
| MS.gene055751 | MS.gene84043 | 0.880491 | 5.25E-70 | -1.69E-46 |
| MS.gene055776 | MS.gene84043 | 0.857937 | 1.16E-62 | -1.69E-46 |
| MS.gene055849 | MS.gene84043 | 0.833816 | 4.29E-56 | -1.69E-46 |
| MS.gene05589 | MS.gene84043 | 0.879754 | 9.62E-70 | -1.69E-46 |
| MS.gene055941 | MS.gene84043 | 0.860899 | 1.50E-63 | -1.69E-46 |
| MS.gene056046 | MS.gene84043 | 0.825423 | 4.73E-54 | -1.69E-46 |
| MS.gene056311 | MS.gene84043 | -0.807697 | 4.45E-50 | -1.69E-46 |
| MS.gene056374 | MS.gene84043 | 0.821349 | 4.23E-53 | -1.69E-46 |
| MS.gene056409 | MS.gene84043 | 0.854829 | 9.49E-62 | -1.69E-46 |
| MS.gene056449 | MS.gene84043 | 0.856811 | 2.50E-62 | -1.69E-46 |
| MS.gene056450 | MS.gene84043 | 0.883846 | 3.18E-71 | -1.69E-46 |
| MS.gene056504 | MS.gene84043 | 0.907764 | 3.56E-81 | -1.69E-46 |
| MS.gene056510 | MS.gene84043 | 0.901842 | 1.78E-78 | -1.69E-46 |
| MS.gene056549 | MS.gene84043 | 0.833603 | 4.85E-56 | -1.69E-46 |
| MS.gene056610 | MS.gene84043 | 0.83523 | 1.89E-56 | -1.69E-46 |
| MS.gene056657 | MS.gene84043 | 0.887471 | 1.39E-72 | -1.69E-46 |
| MS.gene056692 | MS.gene84043 | 0.873524 | 1.38E-67 | -1.69E-46 |
| MS.gene056878 | MS.gene84043 | 0.832804 | 7.67E-56 | -1.69E-46 |
| MS.gene056898 | MS.gene84043 | 0.804152 | 2.48E-49 | -1.69E-46 |
| MS.gene056946 | MS.gene84043 | 0.809646 | 1.71E-50 | -1.69E-46 |
| MS.gene057014 | MS.gene84043 | -0.818523 | 1.88E-52 | -1.69E-46 |
| MS.gene057076 | MS.gene84043 | 0.817228 | 3.68E-52 | -1.69E-46 |
| MS.gene057416 | MS.gene84043 | 0.809862 | 1.53E-50 | -1.69E-46 |
| MS.gene057687 | MS.gene84043 | 0.814753 | 1.31E-51 | -1.69E-46 |
| MS.gene057831 | MS.gene84043 | 0.879748 | 9.67E-70 | -1.69E-46 |
| MS.gene057857 | MS.gene84043 | 0.805068 | 1.60E-49 | -1.69E-46 |
| MS.gene057872 | MS.gene84043 | 0.848602 | 5.52E-60 | -1.69E-46 |
| MS.gene05787 | MS.gene84043 | 0.847978 | 8.21E-60 | -1.69E-46 |
| MS.gene058068 | MS.gene84043 | -0.805209 | 1.49E-49 | -1.69E-46 |
| MS.gene058157 | MS.gene84043 | 0.831031 | 2.10E-55 | -1.69E-46 |
| MS.gene058171 | MS.gene84043 | -0.808497 | 3.01E-50 | -1.69E-46 |
| MS.gene058175 | MS.gene84043 | -0.803993 | 2.67E-49 | -1.69E-46 |
| MS.gene058334 | MS.gene84043 | -0.800909 | 1.16E-48 | -1.69E-46 |
| MS.gene058335 | MS.gene84043 | 0.839197 | 1.83E-57 | -1.69E-46 |
| MS.gene058338 | MS.gene84043 | 0.828146 | 1.06E-54 | -1.69E-46 |
| MS.gene05837 | MS.gene84043 | 0.866167 | 3.48E-65 | -1.69E-46 |
| MS.gene058419 | MS.gene84043 | 0.87997 | 8.06E-70 | -1.69E-46 |
| MS.gene058544 | MS.gene84043 | 0.832354 | 9.92E-56 | -1.69E-46 |
| MS.gene058719 | MS.gene84043 | 0.882398 | 1.08E-70 | -1.69E-46 |
| MS.gene058773 | MS.gene84043 | 0.887178 | 1.79E-72 | -1.69E-46 |
| MS.gene058838 | MS.gene84043 | 0.816357 | 5.76E-52 | -1.69E-46 |
| MS.gene058978 | MS.gene84043 | -0.810818 | 9.54E-51 | -1.69E-46 |
| MS.gene059022 | MS.gene84043 | 0.90817 | 2.29E-81 | -1.69E-46 |
| MS.gene059023 | MS.gene84043 | 0.881724 | 1.89E-70 | -1.69E-46 |
| MS.gene059029 | MS.gene84043 | 0.810396 | 1.18E-50 | -1.69E-46 |
| MS.gene059052 | MS.gene84043 | -0.808303 | 3.31E-50 | -1.69E-46 |
| MS.gene05912 | MS.gene84043 | 0.854525 | 1.16E-61 | -1.69E-46 |
| MS.gene05913 | MS.gene84043 | 0.825954 | 3.54E-54 | -1.69E-46 |
| MS.gene059367 | MS.gene84043 | 0.838453 | 2.85E-57 | -1.69E-46 |
| MS.gene059677 | MS.gene84043 | 0.892401 | 1.64E-74 | -1.69E-46 |
| MS.gene059718 | MS.gene84043 | 0.876843 | 1.01E-68 | -1.69E-46 |
| MS.gene059756 | MS.gene84043 | 0.851874 | 6.68E-61 | -1.69E-46 |
| MS.gene059801 | MS.gene84043 | 0.873553 | 1.34E-67 | -1.69E-46 |
| MS.gene059837 | MS.gene84043 | 0.874252 | 7.81E-68 | -1.69E-46 |
| MS.gene059865 | MS.gene84043 | 0.886102 | 4.58E-72 | -1.69E-46 |
| MS.gene059902 | MS.gene84043 | 0.809744 | 1.63E-50 | -1.69E-46 |
| MS.gene06000 | MS.gene84043 | 0.868524 | 6.12E-66 | -1.69E-46 |
| MS.gene060187 | MS.gene84043 | -0.803854 | 2.86E-49 | -1.69E-46 |
| MS.gene060339 | MS.gene84043 | 0.802722 | 4.90E-49 | -1.69E-46 |
| MS.gene060482 | MS.gene84043 | 0.807252 | 5.53E-50 | -1.69E-46 |
| MS.gene060526 | MS.gene84043 | 0.803241 | 3.83E-49 | -1.69E-46 |
| MS.gene060595 | MS.gene84043 | -0.866359 | 3.02E-65 | -1.69E-46 |
| MS.gene060602 | MS.gene84043 | -0.807075 | 6.03E-50 | -1.69E-46 |
| MS.gene060703 | MS.gene84043 | 0.909348 | 6.29E-82 | -1.69E-46 |
| MS.gene060762 | MS.gene84043 | 0.889674 | 1.96E-73 | -1.69E-46 |
| MS.gene060778 | MS.gene84043 | 0.905642 | 3.46E-80 | -1.69E-46 |
| MS.gene06077 | MS.gene84043 | 0.924358 | 7.84E-90 | -1.69E-46 |
| MS.gene06092 | MS.gene84043 | 0.810825 | 9.51E-51 | -1.69E-46 |
| MS.gene060982 | MS.gene84043 | 0.890328 | 1.09E-73 | -1.69E-46 |
| MS.gene061150 | MS.gene84043 | 0.850552 | 1.58E-60 | -1.69E-46 |
| MS.gene061171 | MS.gene84043 | 0.849025 | 4.21E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84043.t1 | MTR_1g050530 | 98.529 | 204 | 3 | 0 | 1 | 204 | 1 | 204 | 1.89e-146 | 406 |
| MS.gene84043.t1 | MTR_1g050530 | 97.605 | 167 | 4 | 0 | 1 | 167 | 1 | 167 | 1.07e-117 | 332 |
| MS.gene84043.t1 | MTR_1g010080 | 24.038 | 208 | 137 | 6 | 12 | 204 | 25 | 226 | 4.44e-16 | 74.7 |
| MS.gene84043.t1 | MTR_1g010080 | 24.607 | 191 | 124 | 5 | 29 | 204 | 9 | 194 | 1.58e-15 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene84043.t1 | AT5G04440 | 76.608 | 171 | 40 | 0 | 34 | 204 | 63 | 233 | 1.91e-96 | 280 |
| MS.gene84043.t1 | AT5G04440 | 76.608 | 171 | 40 | 0 | 34 | 204 | 63 | 233 | 2.82e-96 | 280 |
| MS.gene84043.t1 | AT4G31115 | 28.161 | 174 | 113 | 4 | 34 | 198 | 54 | 224 | 1.56e-19 | 84.0 |
| MS.gene84043.t1 | AT4G31115 | 28.161 | 174 | 113 | 4 | 34 | 198 | 78 | 248 | 2.72e-19 | 83.6 |
Find 48 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGATTCAATTGCTTGCTT+TGG | 0.335311 | 1.3:+32527109 | None:intergenic |
| AACACTGAATATTGACTTGC+TGG | 0.347727 | 1.3:+32528074 | None:intergenic |
| AACAAACCTGTGACATAAAA+CGG | 0.350906 | 1.3:+32527044 | None:intergenic |
| GAGTACTACCACCTCAATTA+TGG | 0.352926 | 1.3:+32527248 | None:intergenic |
| CAAGAAGTGGAAGGCGTTGT+TGG | 0.383157 | 1.3:+32528286 | None:intergenic |
| AAAGTAGGTACCTACCTTGC+AGG | 0.389681 | 1.3:+32527908 | None:intergenic |
| ACAAACCTGTGACATAAAAC+GGG | 0.400266 | 1.3:+32527045 | None:intergenic |
| TCAACTTTGACAAGCAAAAC+AGG | 0.418135 | 1.3:+32527966 | None:intergenic |
| AATTGGGATGGCTTCGTCAT+TGG | 0.418577 | 1.3:-32528352 | None:intergenic |
| GAGTTGATGACAACGTGAAA+TGG | 0.422343 | 1.3:+32528308 | None:intergenic |
| GAGCTTAATGCAACAGCCAT+TGG | 0.425284 | 1.3:+32527935 | None:intergenic |
| ATGTGGTAATGCAGCTATAA+TGG | 0.425880 | 1.3:-32527334 | MS.gene84043:intron |
| TTTCTTACATTACAGCTTCA+GGG | 0.427767 | 1.3:-32527835 | MS.gene84043:intron |
| AATTTGTAGTTGAATATATG+AGG | 0.428648 | 1.3:-32528100 | MS.gene84043:intron |
| TAATCTTGAGTATTTGTTCA+AGG | 0.430233 | 1.3:+32527074 | None:intergenic |
| AAGCAAGCAATTGAATCAGC+TGG | 0.447176 | 1.3:-32527106 | MS.gene84043:CDS |
| AGTCACTTTCACACGATATC+CGG | 0.455952 | 1.3:+32527307 | None:intergenic |
| AGCAACGAAACGGGCTTTGT+GGG | 0.456712 | 1.3:+32528242 | None:intergenic |
| CGTTCTGAGCAGCGACCATA+GGG | 0.460731 | 1.3:+32527809 | None:intergenic |
| GCTCAGAACGACAAGTTTGA+TGG | 0.461254 | 1.3:-32527796 | MS.gene84043:intron |
| AGCTTAATGCAACAGCCATT+GGG | 0.464658 | 1.3:+32527936 | None:intergenic |
| GAGCAACGAAACGGGCTTTG+TGG | 0.479564 | 1.3:+32528241 | None:intergenic |
| CTTACGGCGAGCAACGAAAC+GGG | 0.479910 | 1.3:+32528233 | None:intergenic |
| TCGTTCTGAGCAGCGACCAT+AGG | 0.486521 | 1.3:+32527808 | None:intergenic |
| ATGCAGCTATAATGGTGAAC+CGG | 0.501075 | 1.3:-32527326 | MS.gene84043:intron |
| TGAAATGGGTTGAACAAGAA+TGG | 0.501639 | 1.3:+32528323 | None:intergenic |
| CCGTTTCGTTGCTCGCCGTA+AGG | 0.506017 | 1.3:-32528232 | MS.gene84043:CDS |
| GAACAGAAACAGACTCCTTA+CGG | 0.507973 | 1.3:+32528217 | None:intergenic |
| GGTATTGCTTTGAAAGCGAA+AGG | 0.511262 | 1.3:+32527130 | None:intergenic |
| CCTTACGGCGAGCAACGAAA+CGG | 0.521735 | 1.3:+32528232 | None:intergenic |
| TTCTTACATTACAGCTTCAG+GGG | 0.527869 | 1.3:-32527834 | MS.gene84043:intron |
| ATTCAGTGTTGGACGCTGAA+AGG | 0.542698 | 1.3:-32528061 | MS.gene84043:CDS |
| ACAGCTTCAGGGGTCCCCTA+TGG | 0.546446 | 1.3:-32527824 | MS.gene84043:intron |
| AGTTGATGACAACGTGAAAT+GGG | 0.550183 | 1.3:+32528309 | None:intergenic |
| AGCAAGCAATTGAATCAGCT+GGG | 0.564610 | 1.3:-32527105 | MS.gene84043:CDS |
| ACTACTTACTTAGGGGACGT+TGG | 0.565216 | 1.3:+32528187 | None:intergenic |
| CACATCAGACACCATAATTG+AGG | 0.567823 | 1.3:-32527259 | MS.gene84043:intron |
| CATTAAGCTCTTATCCTGCA+AGG | 0.575460 | 1.3:-32527922 | MS.gene84043:intron |
| AAACGGGCTTTGTGGGTCGA+AGG | 0.589377 | 1.3:+32528249 | None:intergenic |
| GGAGTTGCTGAAGCAAGAAG+TGG | 0.592610 | 1.3:+32528273 | None:intergenic |
| CGGGCTTTGTGGGTCGAAGG+AGG | 0.597317 | 1.3:+32528252 | None:intergenic |
| AGCAAGTCAATATTCAGTGT+TGG | 0.598943 | 1.3:-32528072 | MS.gene84043:CDS |
| AAGTGACTCAGATAAGTCAC+TGG | 0.624597 | 1.3:-32527292 | MS.gene84043:CDS |
| TTGCTGAAGCAAGAAGTGGA+AGG | 0.666859 | 1.3:+32528277 | None:intergenic |
| AAAGTTGAACAACAACCCAA+TGG | 0.669566 | 1.3:-32527951 | MS.gene84043:CDS |
| CAAACCTGTGACATAAAACG+GGG | 0.689169 | 1.3:+32527046 | None:intergenic |
| AAGGATTGAACGTGTGAGTG+AGG | 0.702772 | 1.3:-32528042 | MS.gene84043:CDS |
| GTTCTGAGCAGCGACCATAG+GGG | 0.774414 | 1.3:+32527810 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAATAAAAATCAGAAGAAA+AGG | + | chr1.3:32528176-32528195 | None:intergenic | 15.0% |
| !! | AAGAAAACAAGAAATAAGAA+AGG | + | chr1.3:32527538-32527557 | None:intergenic | 20.0% |
| !! | AATTTGTAGTTGAATATATG+AGG | - | chr1.3:32527287-32527306 | MS.gene84043:CDS | 20.0% |
| !! | AGAAAACAAGAAATAAGAAA+GGG | + | chr1.3:32527537-32527556 | None:intergenic | 20.0% |
| !!! | AATTAAGTTTTTCACATTTG+AGG | - | chr1.3:32527393-32527412 | MS.gene84043:intron | 20.0% |
| !!! | TTGATAATACTACTTACTTA+GGG | + | chr1.3:32527211-32527230 | None:intergenic | 20.0% |
| ! | AAAGGGTGAAAAAGAAAATA+TGG | + | chr1.3:32527520-32527539 | None:intergenic | 25.0% |
| ! | AAGGGTGAAAAAGAAAATAT+GGG | + | chr1.3:32527519-32527538 | None:intergenic | 25.0% |
| ! | ACGGTGATTTCAAAATTATA+TGG | - | chr1.3:32527900-32527919 | MS.gene84043:intron | 25.0% |
| ! | GTCATTTCAATACTTTAATG+TGG | - | chr1.3:32528036-32528055 | MS.gene84043:CDS | 25.0% |
| ! | GTGTTAATGATAAGTAAAGT+AGG | + | chr1.3:32527497-32527516 | None:intergenic | 25.0% |
| ! | TAATCTTGAGTATTTGTTCA+AGG | + | chr1.3:32528316-32528335 | None:intergenic | 25.0% |
| ! | TGATAATACTACTTACTTAG+GGG | + | chr1.3:32527210-32527229 | None:intergenic | 25.0% |
| !! | CTTGATAATACTACTTACTT+AGG | + | chr1.3:32527212-32527231 | None:intergenic | 25.0% |
| AACAAACCTGTGACATAAAA+CGG | + | chr1.3:32528346-32528365 | None:intergenic | 30.0% | |
| AAGGGAAAAAGGAAATTTGT+AGG | - | chr1.3:32527975-32527994 | MS.gene84043:CDS | 30.0% | |
| AGTAAATGTGAATGTAGCTA+AGG | - | chr1.3:32527871-32527890 | MS.gene84043:intron | 30.0% | |
| AGTAACAGTCAAAGAATACA+TGG | + | chr1.3:32527765-32527784 | None:intergenic | 30.0% | |
| TTTCTTACATTACAGCTTCA+GGG | - | chr1.3:32527552-32527571 | MS.gene84043:intron | 30.0% | |
| ! | GATGACTTGTATTATAAAGC+TGG | - | chr1.3:32527681-32527700 | MS.gene84043:intron | 30.0% |
| ! | TTTTCTTACATTACAGCTTC+AGG | - | chr1.3:32527551-32527570 | MS.gene84043:intron | 30.0% |
| !! | ATTTGATTCATGCTACTTTC+AGG | - | chr1.3:32528010-32528029 | MS.gene84043:CDS | 30.0% |
| AAAGTTGAACAACAACCCAA+TGG | - | chr1.3:32527436-32527455 | MS.gene84043:intron | 35.0% | |
| AAATGTAGACTCGTGATACA+AGG | - | chr1.3:32527798-32527817 | MS.gene84043:CDS | 35.0% | |
| AAGATCAGACAACTCTATCA+TGG | + | chr1.3:32527717-32527736 | None:intergenic | 35.0% | |
| AATGTAGCTAAGGATGGTTA+CGG | - | chr1.3:32527881-32527900 | MS.gene84043:intron | 35.0% | |
| AATGTGAATGTAGCTAAGGA+TGG | - | chr1.3:32527875-32527894 | MS.gene84043:intron | 35.0% | |
| ACAAACCTGTGACATAAAAC+GGG | + | chr1.3:32528345-32528364 | None:intergenic | 35.0% | |
| AGCAAGTCAATATTCAGTGT+TGG | - | chr1.3:32527315-32527334 | MS.gene84043:CDS | 35.0% | |
| TACCTCTACTTGAGTAAACA+TGG | + | chr1.3:32527932-32527951 | None:intergenic | 35.0% | |
| TCAAAGAATACATGGAGCAA+GGG | + | chr1.3:32527757-32527776 | None:intergenic | 35.0% | |
| TCAACTTTGACAAGCAAAAC+AGG | + | chr1.3:32527424-32527443 | None:intergenic | 35.0% | |
| TTCTTACATTACAGCTTCAG+GGG | - | chr1.3:32527553-32527572 | MS.gene84043:intron | 35.0% | |
| ! | ATGTGGTAATGCAGCTATAA+TGG | - | chr1.3:32528053-32528072 | MS.gene84043:CDS | 35.0% |
| ! | CTTTTTCCCTTTCTCAGAAA+GGG | + | chr1.3:32527966-32527985 | None:intergenic | 35.0% |
| ! | TCTTGTTACTTGTCGAATCT+CGG | - | chr1.3:32527637-32527656 | MS.gene84043:intron | 35.0% |
| ! | TGTATCACGAGTCTACATTT+TGG | + | chr1.3:32527798-32527817 | None:intergenic | 35.0% |
| !! | AACACTGAATATTGACTTGC+TGG | + | chr1.3:32527316-32527335 | None:intergenic | 35.0% |
| !! | AGTTGATGACAACGTGAAAT+GGG | + | chr1.3:32527081-32527100 | None:intergenic | 35.0% |
| !! | TGAAATGGGTTGAACAAGAA+TGG | + | chr1.3:32527067-32527086 | None:intergenic | 35.0% |
| AAGCAAGCAATTGAATCAGC+TGG | - | chr1.3:32528281-32528300 | MS.gene84043:CDS | 40.0% | |
| AAGTGACTCAGATAAGTCAC+TGG | - | chr1.3:32528095-32528114 | MS.gene84043:intron | 40.0% | |
| AGCAAGCAATTGAATCAGCT+GGG | - | chr1.3:32528282-32528301 | MS.gene84043:CDS | 40.0% | |
| AGCTTAATGCAACAGCCATT+GGG | + | chr1.3:32527454-32527473 | None:intergenic | 40.0% | |
| ATCAGACACCATAATTGAGG+TGG | - | chr1.3:32528131-32528150 | MS.gene84043:intron | 40.0% | |
| ATGCAGCTATAATGGTGAAC+CGG | - | chr1.3:32528061-32528080 | MS.gene84043:CDS | 40.0% | |
| CAAACCTGTGACATAAAACG+GGG | + | chr1.3:32528344-32528363 | None:intergenic | 40.0% | |
| CACATCAGACACCATAATTG+AGG | - | chr1.3:32528128-32528147 | MS.gene84043:intron | 40.0% | |
| CATTAAGCTCTTATCCTGCA+AGG | - | chr1.3:32527465-32527484 | MS.gene84043:intron | 40.0% | |
| CTCCATGTTTACTCAAGTAG+AGG | - | chr1.3:32527927-32527946 | MS.gene84043:CDS | 40.0% | |
| CTCTACTTGAGTAAACATGG+AGG | + | chr1.3:32527929-32527948 | None:intergenic | 40.0% | |
| GAACAGAAACAGACTCCTTA+CGG | + | chr1.3:32527173-32527192 | None:intergenic | 40.0% | |
| GAGTACTACCACCTCAATTA+TGG | + | chr1.3:32528142-32528161 | None:intergenic | 40.0% | |
| GTCAAAGAATACATGGAGCA+AGG | + | chr1.3:32527758-32527777 | None:intergenic | 40.0% | |
| TGCATTGCTATGTCGAATTC+AGG | - | chr1.3:32528220-32528239 | MS.gene84043:CDS | 40.0% | |
| ! | AGTCACTTTCACACGATATC+CGG | + | chr1.3:32528083-32528102 | None:intergenic | 40.0% |
| ! | CCTTTCTGAGAAAGGGAAAA+AGG | - | chr1.3:32527964-32527983 | MS.gene84043:CDS | 40.0% |
| ! | CCTTTTTCCCTTTCTCAGAA+AGG | + | chr1.3:32527967-32527986 | None:intergenic | 40.0% |
| ! | GCTGATTCAATTGCTTGCTT+TGG | + | chr1.3:32528281-32528300 | None:intergenic | 40.0% |
| !! | GAGTTGATGACAACGTGAAA+TGG | + | chr1.3:32527082-32527101 | None:intergenic | 40.0% |
| !! | GGTATTGCTTTGAAAGCGAA+AGG | + | chr1.3:32528260-32528279 | None:intergenic | 40.0% |
| AAAGTAGGTACCTACCTTGC+AGG | + | chr1.3:32527482-32527501 | None:intergenic | 45.0% | |
| AAGCTCTTATCCTGCAAGGT+AGG | - | chr1.3:32527469-32527488 | MS.gene84043:intron | 45.0% | |
| AAGGATTGAACGTGTGAGTG+AGG | - | chr1.3:32527345-32527364 | MS.gene84043:intron | 45.0% | |
| ACTACTTACTTAGGGGACGT+TGG | + | chr1.3:32527203-32527222 | None:intergenic | 45.0% | |
| GAGCTTAATGCAACAGCCAT+TGG | + | chr1.3:32527455-32527474 | None:intergenic | 45.0% | |
| GCTCAGAACGACAAGTTTGA+TGG | - | chr1.3:32527591-32527610 | MS.gene84043:intron | 45.0% | |
| GTAGACTCGTGATACAAGGA+GGG | - | chr1.3:32527802-32527821 | MS.gene84043:CDS | 45.0% | |
| GTCGAATCTCGGTCATTAGT+TGG | - | chr1.3:32527648-32527667 | MS.gene84043:intron | 45.0% | |
| TGTAGACTCGTGATACAAGG+AGG | - | chr1.3:32527801-32527820 | MS.gene84043:CDS | 45.0% | |
| TTGCTGAAGCAAGAAGTGGA+AGG | + | chr1.3:32527113-32527132 | None:intergenic | 45.0% | |
| ! | ATTCAGTGTTGGACGCTGAA+AGG | - | chr1.3:32527326-32527345 | MS.gene84043:intron | 45.0% |
| ! | CTCGTTCCCTTTCTGAGAAA+GGG | - | chr1.3:32527957-32527976 | MS.gene84043:CDS | 45.0% |
| ! | TCTCGTTCCCTTTCTGAGAA+AGG | - | chr1.3:32527956-32527975 | MS.gene84043:CDS | 45.0% |
| GAGCAAGGGATCTCGATAGT+TGG | + | chr1.3:32527743-32527762 | None:intergenic | 50.0% | |
| GGAGTTGCTGAAGCAAGAAG+TGG | + | chr1.3:32527117-32527136 | None:intergenic | 50.0% | |
| ! | AGCAACGAAACGGGCTTTGT+GGG | + | chr1.3:32527148-32527167 | None:intergenic | 50.0% |
| ! | GCTTCCCCGTTTTATGTCAC+AGG | - | chr1.3:32528337-32528356 | MS.gene84043:CDS | 50.0% |
| !! | CAAGAAGTGGAAGGCGTTGT+TGG | + | chr1.3:32527104-32527123 | None:intergenic | 50.0% |
| CCTTACGGCGAGCAACGAAA+CGG | + | chr1.3:32527158-32527177 | None:intergenic | 55.0% | |
| CTTACGGCGAGCAACGAAAC+GGG | + | chr1.3:32527157-32527176 | None:intergenic | 55.0% | |
| ! | AAACGGGCTTTGTGGGTCGA+AGG | + | chr1.3:32527141-32527160 | None:intergenic | 55.0% |
| ! | CGTTCTGAGCAGCGACCATA+GGG | + | chr1.3:32527581-32527600 | None:intergenic | 55.0% |
| ! | GAGCAACGAAACGGGCTTTG+TGG | + | chr1.3:32527149-32527168 | None:intergenic | 55.0% |
| ! | GTTCTGAGCAGCGACCATAG+GGG | + | chr1.3:32527580-32527599 | None:intergenic | 55.0% |
| ! | TCGTTCTGAGCAGCGACCAT+AGG | + | chr1.3:32527582-32527601 | None:intergenic | 55.0% |
| ACAGCTTCAGGGGTCCCCTA+TGG | - | chr1.3:32527563-32527582 | MS.gene84043:intron | 60.0% | |
| CCGTTTCGTTGCTCGCCGTA+AGG | - | chr1.3:32527155-32527174 | MS.gene84043:intron | 60.0% | |
| ! | CGGGCTTTGTGGGTCGAAGG+AGG | + | chr1.3:32527138-32527157 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 32527042 | 32528367 | 32527042 | ID=MS.gene84043 |
| chr1.3 | mRNA | 32527042 | 32528367 | 32527042 | ID=MS.gene84043.t1;Parent=MS.gene84043 |
| chr1.3 | exon | 32528196 | 32528367 | 32528196 | ID=MS.gene84043.t1.exon1;Parent=MS.gene84043.t1 |
| chr1.3 | CDS | 32528196 | 32528367 | 32528196 | ID=cds.MS.gene84043.t1;Parent=MS.gene84043.t1 |
| chr1.3 | exon | 32527923 | 32528113 | 32527923 | ID=MS.gene84043.t1.exon2;Parent=MS.gene84043.t1 |
| chr1.3 | CDS | 32527923 | 32528113 | 32527923 | ID=cds.MS.gene84043.t1;Parent=MS.gene84043.t1 |
| chr1.3 | exon | 32527797 | 32527842 | 32527797 | ID=MS.gene84043.t1.exon3;Parent=MS.gene84043.t1 |
| chr1.3 | CDS | 32527797 | 32527842 | 32527797 | ID=cds.MS.gene84043.t1;Parent=MS.gene84043.t1 |
| chr1.3 | exon | 32527260 | 32527342 | 32527260 | ID=MS.gene84043.t1.exon4;Parent=MS.gene84043.t1 |
| chr1.3 | CDS | 32527260 | 32527342 | 32527260 | ID=cds.MS.gene84043.t1;Parent=MS.gene84043.t1 |
| chr1.3 | exon | 32527042 | 32527167 | 32527042 | ID=MS.gene84043.t1.exon5;Parent=MS.gene84043.t1 |
| chr1.3 | CDS | 32527042 | 32527167 | 32527042 | ID=cds.MS.gene84043.t1;Parent=MS.gene84043.t1 |
| Gene Sequence |
| Protein sequence |
