AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene066192


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066192.t1 MTR_2g064370 95.506 267 9 1 1 264 1 267 0.0 513
MS.gene066192.t1 MTR_8g046460 71.654 254 66 3 14 264 18 268 2.72e-133 377
MS.gene066192.t1 MTR_8g028425 57.792 154 63 1 75 226 92 245 9.71e-65 202
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene066192.t1 AT1G79510 65.091 275 85 6 1 264 1 275 6.50e-120 343
MS.gene066192.t1 AT1G79510 65.091 275 85 6 1 264 1 275 6.50e-120 343
MS.gene066192.t1 AT1G16320 59.489 274 100 6 1 264 1 273 4.11e-110 319
MS.gene066192.t1 AT2G46220 55.414 157 68 1 72 226 81 237 5.61e-65 203

Find 60 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGTACAAATTGATCTTC+TGG 0.216595 2.2:+25376378 MS.gene066192:CDS
GACCCTTTGAACACATTTAC+TGG 0.221962 2.2:+25376350 MS.gene066192:CDS
AGGCACTTCAAAGTATAAAT+TGG 0.231544 2.2:+25376547 MS.gene066192:CDS
TATTAAACCAGTTTCAGTTT+TGG 0.296641 2.2:+25376631 MS.gene066192:CDS
AGAAAATGTGAAGTAGGTTT+TGG 0.326406 2.2:-25375569 None:intergenic
TTCATGGATAACTTTATATA+AGG 0.327641 2.2:+25376724 MS.gene066192:CDS
TTCTGGGCATTGAGGTTTCA+TGG 0.332313 2.2:+25376395 MS.gene066192:CDS
TAACTTTATATAAGGCAGTT+AGG 0.342291 2.2:+25376732 MS.gene066192:CDS
AGGGCGTGCCGCGTGTCCCT+TGG 0.359335 2.2:+25376504 MS.gene066192:CDS
TTGGTGAAGAGGTTTGTCTT+TGG 0.362955 2.2:-25375534 None:intergenic
GAGTTGGGAGATAAGGTTGT+AGG 0.369415 2.2:-25375502 None:intergenic
ACAAAATCCAAAACTGAAAC+TGG 0.373146 2.2:-25376638 None:intergenic
GGGAGATAAGGTTGTAGGTT+TGG 0.374951 2.2:-25375497 None:intergenic
GGGCGTGCCGCGTGTCCCTT+GGG 0.391041 2.2:+25376505 MS.gene066192:CDS
CGAAGAATGTGATATGGATT+AGG 0.391674 2.2:+25376471 MS.gene066192:CDS
TTTGATTGAGTTGGGAGATA+AGG 0.392649 2.2:-25375509 None:intergenic
ATGGATTAGGTGGAAACTTA+AGG 0.394506 2.2:+25376484 MS.gene066192:CDS
TGGAGTTTGAAGGGTGGCTA+AGG 0.408366 2.2:-25375624 None:intergenic
AAAGTACAAATTGATCTTCT+GGG 0.411012 2.2:+25376379 MS.gene066192:CDS
TGGATGTCTACTCTTCTTCA+TGG 0.415651 2.2:+25376708 MS.gene066192:CDS
TTGACATAGAATTCATCCTT+TGG 0.425313 2.2:-25375692 None:intergenic
AATGTAGGGTTTGGACTAGC+AGG 0.427331 2.2:-25376671 None:intergenic
AACTTTATATAAGGCAGTTA+GGG 0.433549 2.2:+25376733 MS.gene066192:CDS
AGGTCTTTGATGAAGATCAT+AGG 0.436047 2.2:-25375752 None:intergenic
TGTTTATCTTGAACACTTGA+CGG 0.437362 2.2:-25375665 None:intergenic
AACCTTCGAAGAATGTGATA+TGG 0.437736 2.2:+25376465 MS.gene066192:CDS
TGAACATAAAGTTGATAACT+TGG 0.441163 2.2:+25376589 MS.gene066192:CDS
TGGATTAGGTGGAAACTTAA+GGG 0.463830 2.2:+25376485 MS.gene066192:CDS
ATTCCAGTAAATGTGTTCAA+AGG 0.467787 2.2:-25376353 None:intergenic
AGAAGAAGAATCAGATGAAG+TGG 0.472139 2.2:-25375600 None:intergenic
TTCCAGTAAATGTGTTCAAA+GGG 0.481927 2.2:-25376352 None:intergenic
ACCTCAATTATGATATCTAT+AGG 0.485916 2.2:+25375771 MS.gene066192:CDS
AGGGAGACTTTGAAACAAGA+AGG 0.486403 2.2:+25376752 MS.gene066192:CDS
GATACAAGAAAATGTGAAGT+AGG 0.489505 2.2:-25375575 None:intergenic
GGAGTTTGAAGGGTGGCTAA+GGG 0.505819 2.2:-25375623 None:intergenic
AATTGATCTTCTGGGCATTG+AGG 0.508643 2.2:+25376387 MS.gene066192:CDS
GAAGCTAAAGGAGAATTTCA+AGG 0.512138 2.2:+25376527 MS.gene066192:CDS
AAGTATAAATTGGACAAGAA+TGG 0.514460 2.2:+25376557 MS.gene066192:CDS
ACCTATAGATATCATAATTG+AGG 0.529058 2.2:-25375772 None:intergenic
GATGAATTCTATGTCAACCT+TGG 0.529105 2.2:+25375698 MS.gene066192:CDS
CGTGTCCCTTGGGAAGCTAA+AGG 0.549445 2.2:+25376515 MS.gene066192:CDS
AATTCTCCTTTAGCTTCCCA+AGG 0.554730 2.2:-25376521 None:intergenic
TTGTGAAGTTGGAGTTTGAA+GGG 0.561912 2.2:-25375633 None:intergenic
CTTGTGAAGTTGGAGTTTGA+AGG 0.564715 2.2:-25375634 None:intergenic
ATAGATAATACTTACGAGCA+TGG 0.565339 2.2:-25376803 None:intergenic
ATCCATATCACATTCTTCGA+AGG 0.567194 2.2:-25376467 None:intergenic
GGTGTGTTAACTTGTGAAGT+TGG 0.574807 2.2:-25375644 None:intergenic
AGAATGTGATATGGATTAGG+TGG 0.583307 2.2:+25376474 MS.gene066192:CDS
GAAGAAGAGTAGACATCCAC+AGG 0.583593 2.2:-25376704 None:intergenic
GGGAGACTTTGAAACAAGAA+GGG 0.595464 2.2:+25376753 MS.gene066192:CDS
GAGTTCTAACAGCAAGTCCA+AGG 0.595497 2.2:-25375715 None:intergenic
GATGAAAGAGTTTGGTGAAG+AGG 0.606204 2.2:-25375545 None:intergenic
GGGAGTTTGCTACCACAAGA+TGG 0.611323 2.2:+25376773 MS.gene066192:CDS
ATTCTCCTTTAGCTTCCCAA+GGG 0.617667 2.2:-25376520 None:intergenic
ATGTAGGGTTTGGACTAGCA+GGG 0.639151 2.2:-25376670 None:intergenic
TTAGCTTCCCAAGGGACACG+CGG 0.642123 2.2:-25376512 None:intergenic
CACTTGATGTATATAGAGTG+TGG 0.642892 2.2:+25376441 MS.gene066192:CDS
TCAAGATAAACAGCTTCCAA+AGG 0.645111 2.2:+25375676 MS.gene066192:CDS
CATGGAGCTAGACCATCTTG+TGG 0.685164 2.2:-25376785 None:intergenic
TGAAGTTGGAGTTTGAAGGG+TGG 0.695576 2.2:-25375630 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAGATGTTATAAGAAA+TGG - chr2.2:25375825-25375844 None:intergenic 15.0%
!! TATCAATAGTTTAATACTAT+TGG - chr2.2:25376167-25376186 None:intergenic 15.0%
!! TATTTAATTGTACTATATAC+AGG + chr2.2:25375944-25375963 MS.gene066192:intron 15.0%
!! GTTTATAAAATGAGCTTATT+AGG + chr2.2:25376259-25376278 MS.gene066192:intron 20.0%
!! TGTTAAATAGGCTTATAATA+GGG - chr2.2:25375895-25375914 None:intergenic 20.0%
!! TTACACATTAAGTGTTAAAT+AGG - chr2.2:25375907-25375926 None:intergenic 20.0%
!! TTCATGGATAACTTTATATA+AGG + chr2.2:25376724-25376743 MS.gene066192:CDS 20.0%
!!! GAAACTGGTTTAATATTTTT+GGG - chr2.2:25376626-25376645 None:intergenic 20.0%
!!! TGAAACTGGTTTAATATTTT+TGG - chr2.2:25376627-25376646 None:intergenic 20.0%
!!! TGAAATTTGATCAATTTTCA+GGG + chr2.2:25376310-25376329 MS.gene066192:intron 20.0%
!!! TTATTAGGTTGTGAATTTTA+AGG + chr2.2:25376274-25376293 MS.gene066192:intron 20.0%
!!! TTGAAATTTGATCAATTTTC+AGG + chr2.2:25376309-25376328 MS.gene066192:intron 20.0%
! AAAGTACAAATTGATCTTCT+GGG + chr2.2:25376379-25376398 MS.gene066192:CDS 25.0%
! AACTTTATATAAGGCAGTTA+GGG + chr2.2:25376733-25376752 MS.gene066192:CDS 25.0%
! AAGTATAAATTGGACAAGAA+TGG + chr2.2:25376557-25376576 MS.gene066192:CDS 25.0%
! ACCTATAGATATCATAATTG+AGG - chr2.2:25375775-25375794 None:intergenic 25.0%
! ACCTCAATTATGATATCTAT+AGG + chr2.2:25375771-25375790 MS.gene066192:CDS 25.0%
! GTGTTAAATAGGCTTATAAT+AGG - chr2.2:25375896-25375915 None:intergenic 25.0%
! TAACTTTATATAAGGCAGTT+AGG + chr2.2:25376732-25376751 MS.gene066192:CDS 25.0%
! TTATAAGAAATGGTGAAATG+AGG - chr2.2:25375815-25375834 None:intergenic 25.0%
!! AAAAGTACAAATTGATCTTC+TGG + chr2.2:25376378-25376397 MS.gene066192:CDS 25.0%
!! TATTAAACCAGTTTCAGTTT+TGG + chr2.2:25376631-25376650 MS.gene066192:CDS 25.0%
!!! AGACAAGTTTTTTTATGCTT+AGG + chr2.2:25376050-25376069 MS.gene066192:intron 25.0%
!!! TGAACATAAAGTTGATAACT+TGG + chr2.2:25376589-25376608 MS.gene066192:CDS 25.0%
AATTGTACTATATACAGGAC+CGG + chr2.2:25375949-25375968 MS.gene066192:intron 30.0%
ACAAAATCCAAAACTGAAAC+TGG - chr2.2:25376641-25376660 None:intergenic 30.0%
AGGCACTTCAAAGTATAAAT+TGG + chr2.2:25376547-25376566 MS.gene066192:CDS 30.0%
ATTCCAGTAAATGTGTTCAA+AGG - chr2.2:25376356-25376375 None:intergenic 30.0%
GATACAAGAAAATGTGAAGT+AGG - chr2.2:25375578-25375597 None:intergenic 30.0%
TGTTTATCTTGAACACTTGA+CGG - chr2.2:25375668-25375687 None:intergenic 30.0%
TTCCAGTAAATGTGTTCAAA+GGG - chr2.2:25376355-25376374 None:intergenic 30.0%
TTGACATAGAATTCATCCTT+TGG - chr2.2:25375695-25375714 None:intergenic 30.0%
! AGAAAATGTGAAGTAGGTTT+TGG - chr2.2:25375572-25375591 None:intergenic 30.0%
! CAGGGATGATATAACATTTT+TGG + chr2.2:25376328-25376347 MS.gene066192:intron 30.0%
!!! CTTTGGATTTTGATTGAGTT+GGG - chr2.2:25375520-25375539 None:intergenic 30.0%
!!! TCTTTGGATTTTGATTGAGT+TGG - chr2.2:25375521-25375540 None:intergenic 30.0%
AACCTTCGAAGAATGTGATA+TGG + chr2.2:25376465-25376484 MS.gene066192:CDS 35.0%
AGAAGAAGAATCAGATGAAG+TGG - chr2.2:25375603-25375622 None:intergenic 35.0%
AGGTCTTTGATGAAGATCAT+AGG - chr2.2:25375755-25375774 None:intergenic 35.0%
ATCCATATCACATTCTTCGA+AGG - chr2.2:25376470-25376489 None:intergenic 35.0%
CACTTGATGTATATAGAGTG+TGG + chr2.2:25376441-25376460 MS.gene066192:CDS 35.0%
CGAAGAATGTGATATGGATT+AGG + chr2.2:25376471-25376490 MS.gene066192:CDS 35.0%
GAAGCTAAAGGAGAATTTCA+AGG + chr2.2:25376527-25376546 MS.gene066192:CDS 35.0%
GATGAATTCTATGTCAACCT+TGG + chr2.2:25375698-25375717 MS.gene066192:CDS 35.0%
TCAAGATAAACAGCTTCCAA+AGG + chr2.2:25375676-25375695 MS.gene066192:CDS 35.0%
TTATGCTTAGGAGTTGAACT+TGG + chr2.2:25376062-25376081 MS.gene066192:intron 35.0%
TTGTGAAGTTGGAGTTTGAA+GGG - chr2.2:25375636-25375655 None:intergenic 35.0%
! TCCAAACCCTACATTTTTGT+GGG + chr2.2:25376679-25376698 MS.gene066192:CDS 35.0%
! TGGATTAGGTGGAAACTTAA+GGG + chr2.2:25376485-25376504 MS.gene066192:CDS 35.0%
!! AGAATGTGATATGGATTAGG+TGG + chr2.2:25376474-25376493 MS.gene066192:CDS 35.0%
!! ATGGATTAGGTGGAAACTTA+AGG + chr2.2:25376484-25376503 MS.gene066192:CDS 35.0%
!! TTTGATTGAGTTGGGAGATA+AGG - chr2.2:25375512-25375531 None:intergenic 35.0%
!!! GTTTTGGTGATGAAAGAGTT+TGG - chr2.2:25375556-25375575 None:intergenic 35.0%
!!! TGTTTTGAAAACTCGATGTC+CGG - chr2.2:25375971-25375990 None:intergenic 35.0%
CTTGTGAAGTTGGAGTTTGA+AGG - chr2.2:25375637-25375656 None:intergenic 40.0%
GACCCTTTGAACACATTTAC+TGG + chr2.2:25376350-25376369 MS.gene066192:CDS 40.0%
TGGATGTCTACTCTTCTTCA+TGG + chr2.2:25376708-25376727 MS.gene066192:CDS 40.0%
TTGGTGAAGAGGTTTGTCTT+TGG - chr2.2:25375537-25375556 None:intergenic 40.0%
! AATTCTCCTTTAGCTTCCCA+AGG - chr2.2:25376524-25376543 None:intergenic 40.0%
! AATTGATCTTCTGGGCATTG+AGG + chr2.2:25376387-25376406 MS.gene066192:CDS 40.0%
! ATTCTCCTTTAGCTTCCCAA+GGG - chr2.2:25376523-25376542 None:intergenic 40.0%
! CCAAACCCTACATTTTTGTG+GGG + chr2.2:25376680-25376699 MS.gene066192:CDS 40.0%
! GATGAAAGAGTTTGGTGAAG+AGG - chr2.2:25375548-25375567 None:intergenic 40.0%
! GTCCAAACCCTACATTTTTG+TGG + chr2.2:25376678-25376697 MS.gene066192:CDS 40.0%
!! AGGGAGACTTTGAAACAAGA+AGG + chr2.2:25376752-25376771 MS.gene066192:CDS 40.0%
!! GGGAGACTTTGAAACAAGAA+GGG + chr2.2:25376753-25376772 MS.gene066192:CDS 40.0%
!! GGTGTGTTAACTTGTGAAGT+TGG - chr2.2:25375647-25375666 None:intergenic 40.0%
ACAGGACCCCACAAAAATGT+AGG - chr2.2:25376689-25376708 None:intergenic 45.0%
CAGGACCCCACAAAAATGTA+GGG - chr2.2:25376688-25376707 None:intergenic 45.0%
CCCCACAAAAATGTAGGGTT+TGG - chr2.2:25376683-25376702 None:intergenic 45.0%
GAAGAAGAGTAGACATCCAC+AGG - chr2.2:25376707-25376726 None:intergenic 45.0%
GAGTTGGGAGATAAGGTTGT+AGG - chr2.2:25375505-25375524 None:intergenic 45.0%
GGGAGATAAGGTTGTAGGTT+TGG - chr2.2:25375500-25375519 None:intergenic 45.0%
TGAAGTTGGAGTTTGAAGGG+TGG - chr2.2:25375633-25375652 None:intergenic 45.0%
! GAGTTCTAACAGCAAGTCCA+AGG - chr2.2:25375718-25375737 None:intergenic 45.0%
! TACATTTTTGTGGGGTCCTG+TGG + chr2.2:25376688-25376707 MS.gene066192:CDS 45.0%
!! AATGTAGGGTTTGGACTAGC+AGG - chr2.2:25376674-25376693 None:intergenic 45.0%
!! ATGTAGGGTTTGGACTAGCA+GGG - chr2.2:25376673-25376692 None:intergenic 45.0%
!! TTCTGGGCATTGAGGTTTCA+TGG + chr2.2:25376395-25376414 MS.gene066192:CDS 45.0%
CATGGAGCTAGACCATCTTG+TGG - chr2.2:25376788-25376807 None:intergenic 50.0%
CTATTGGAGTCTAGCGCAAG+TGG - chr2.2:25376151-25376170 None:intergenic 50.0%
GGAGTTTGAAGGGTGGCTAA+GGG - chr2.2:25375626-25375645 None:intergenic 50.0%
TGGAGTTTGAAGGGTGGCTA+AGG - chr2.2:25375627-25375646 None:intergenic 50.0%
!! GGGAGTTTGCTACCACAAGA+TGG + chr2.2:25376773-25376792 MS.gene066192:CDS 50.0%
CGTGTCCCTTGGGAAGCTAA+AGG + chr2.2:25376515-25376534 MS.gene066192:CDS 55.0%
TGGAGTCTAGCGCAAGTGGT+TGG - chr2.2:25376147-25376166 None:intergenic 55.0%
TTAGCTTCCCAAGGGACACG+CGG - chr2.2:25376515-25376534 None:intergenic 55.0%
! GGTTGGTGCATGTGGTGTGT+CGG - chr2.2:25376130-25376149 None:intergenic 55.0%
! GCGCAAGTGGTTGGTGCATG+TGG - chr2.2:25376138-25376157 None:intergenic 60.0%
AGGGCGTGCCGCGTGTCCCT+TGG + chr2.2:25376504-25376523 MS.gene066192:CDS 75.0%
GGGCGTGCCGCGTGTCCCTT+GGG + chr2.2:25376505-25376524 MS.gene066192:CDS 75.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 25375482 25376814 25375482 ID=MS.gene066192
chr2.2 mRNA 25375482 25376814 25375482 ID=MS.gene066192.t1;Parent=MS.gene066192
chr2.2 exon 25375482 25375792 25375482 ID=MS.gene066192.t1.exon1;Parent=MS.gene066192.t1
chr2.2 CDS 25375482 25375792 25375482 ID=cds.MS.gene066192.t1;Parent=MS.gene066192.t1
chr2.2 exon 25376331 25376814 25376331 ID=MS.gene066192.t1.exon2;Parent=MS.gene066192.t1
chr2.2 CDS 25376331 25376814 25376331 ID=cds.MS.gene066192.t1;Parent=MS.gene066192.t1
Gene Sequence

>MS.gene066192

ATGGCTTTTCTTCTACCAAACCTACAACCTTATCTCCCAACTCAATCAAAATCCAAAGACAAACCTCTTCACCAAACTCTTTCATCACCAAAACCTACTTCACATTTTCTTGTATCTTCCACTTCATCTGATTCTTCTTCTCCCTTAGCCACCCTTCAAACTCCAACTTCACAAGTTAACACACCGTCAAGTGTTCAAGATAAACAGCTTCCAAAGGATGAATTCTATGTCAACCTTGGACTTGCTGTTAGAACTCTTCGTGAAGACATGCCTATGATCTTCATCAAAGACCTCAATTATGATATCTATAGGTTATTTGACTATTTCTCACCTCATTTCACCATTTCTTATAACATCTTTTTTTGATTATTTTTGATATATAATTATTCTAACTTTTATTATGTCATCATCCCTATTATAAGCCTATTTAACACTTAATGTGTAAATTTTGTATATGAATATTATTTAATTGTACTATATACAGGACCGGACATCGAGTTTTCAAAACAAAAAAAAAAATTGCACTATATACAAAGATATGTTCAATATTTATTTTTCTTTTCTTCTAAGACAAGTTTTTTTATGCTTAGGAGTTGAACTTGGCATCTCGATATTTACAACACTAGCACACGCAAGAGTGTAACACCGACACACCACATGCACCAACCACTTGCGCTAGACTCCAATAGTATTAAACTATTGATAAATGATGTAATTTTTTTTATAAAAAGATAGCTAGTAATTTGAAATGATGTTTATATTTTTTATTTTTCGTTTGTTTATAAAATGAGCTTATTAGGTTGTGAATTTTAAGGAGCTTATTTTCATTGAAATTTGATCAATTTTCAGGGATGATATAACATTTTTGGACCCTTTGAACACATTTACTGGAATTGAAAAGTACAAATTGATCTTCTGGGCATTGAGGTTTCATGGTAAGCTCTTGTTTCATGATATAGCACTTGATGTATATAGAGTGTGGCAACCTTCGAAGAATGTGATATGGATTAGGTGGAAACTTAAGGGCGTGCCGCGTGTCCCTTGGGAAGCTAAAGGAGAATTTCAAGGCACTTCAAAGTATAAATTGGACAAGAATGGCAAAATTTATGAACATAAAGTTGATAACTTGGCATTCAATTTTCCCAAAAATATTAAACCAGTTTCAGTTTTGGATTTTGTTACTTCATGCCCTGCTAGTCCAAACCCTACATTTTTGTGGGGTCCTGTGGATGTCTACTCTTCTTCATGGATAACTTTATATAAGGCAGTTAGGGAGACTTTGAAACAAGAAGGGAGTTTGCTACCACAAGATGGTCTAGCTCCATGCTCGTAA

Protein sequence

>MS.gene066192.t1

MAFLLPNLQPYLPTQSKSKDKPLHQTLSSPKPTSHFLVSSTSSDSSSPLATLQTPTSQVNTPSSVQDKQLPKDEFYVNLGLAVRTLREDMPMIFIKDLNYDIYRDDITFLDPLNTFTGIEKYKLIFWALRFHGKLLFHDIALDVYRVWQPSKNVIWIRWKLKGVPRVPWEAKGEFQGTSKYKLDKNGKIYEHKVDNLAFNFPKNIKPVSVLDFVTSCPASPNPTFLWGPVDVYSSSWITLYKAVRETLKQEGSLLPQDGLAPCS