Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06768.t1 | XP_013461518.1 | 99.4 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 6.50E-89 | 336.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06768.t1 | A0A072V2G7 | 99.4 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 4.7e-89 | 336.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049232 | MS.gene06768 | 0.837733 | 4.37E-57 | -1.69E-46 |
| MS.gene049356 | MS.gene06768 | 0.836885 | 7.20E-57 | -1.69E-46 |
| MS.gene049369 | MS.gene06768 | 0.856604 | 2.87E-62 | -1.69E-46 |
| MS.gene049396 | MS.gene06768 | 0.981659 | 3.95E-153 | -1.69E-46 |
| MS.gene049609 | MS.gene06768 | 0.839576 | 1.46E-57 | -1.69E-46 |
| MS.gene049633 | MS.gene06768 | 0.805148 | 1.54E-49 | -1.69E-46 |
| MS.gene050170 | MS.gene06768 | 0.814061 | 1.87E-51 | -1.69E-46 |
| MS.gene050216 | MS.gene06768 | 0.830098 | 3.56E-55 | -1.69E-46 |
| MS.gene050323 | MS.gene06768 | 0.833373 | 5.53E-56 | -1.69E-46 |
| MS.gene050499 | MS.gene06768 | 0.827835 | 1.26E-54 | -1.69E-46 |
| MS.gene050502 | MS.gene06768 | 0.872901 | 2.23E-67 | -1.69E-46 |
| MS.gene050593 | MS.gene06768 | 0.818483 | 1.91E-52 | -1.69E-46 |
| MS.gene050662 | MS.gene06768 | 0.826599 | 2.48E-54 | -1.69E-46 |
| MS.gene050686 | MS.gene06768 | 0.827265 | 1.72E-54 | -1.69E-46 |
| MS.gene050697 | MS.gene06768 | 0.815244 | 1.02E-51 | -1.69E-46 |
| MS.gene050772 | MS.gene06768 | 0.842458 | 2.55E-58 | -1.69E-46 |
| MS.gene05080 | MS.gene06768 | 0.852827 | 3.58E-61 | -1.69E-46 |
| MS.gene050868 | MS.gene06768 | 0.833887 | 4.12E-56 | -1.69E-46 |
| MS.gene050871 | MS.gene06768 | 0.837942 | 3.86E-57 | -1.69E-46 |
| MS.gene05105 | MS.gene06768 | 0.830968 | 2.18E-55 | -1.69E-46 |
| MS.gene051268 | MS.gene06768 | 0.813599 | 2.36E-51 | -1.69E-46 |
| MS.gene051509 | MS.gene06768 | 0.854527 | 1.16E-61 | -1.69E-46 |
| MS.gene051734 | MS.gene06768 | 0.839988 | 1.14E-57 | -1.69E-46 |
| MS.gene051838 | MS.gene06768 | 0.803766 | 2.98E-49 | -1.69E-46 |
| MS.gene051874 | MS.gene06768 | 0.862462 | 4.98E-64 | -1.69E-46 |
| MS.gene052164 | MS.gene06768 | 0.8253 | 5.05E-54 | -1.69E-46 |
| MS.gene052346 | MS.gene06768 | 0.819169 | 1.34E-52 | -1.69E-46 |
| MS.gene052414 | MS.gene06768 | 0.839253 | 1.77E-57 | -1.69E-46 |
| MS.gene052506 | MS.gene06768 | 0.805672 | 1.19E-49 | -1.69E-46 |
| MS.gene052822 | MS.gene06768 | 0.816528 | 5.28E-52 | -1.69E-46 |
| MS.gene053018 | MS.gene06768 | 0.806545 | 7.81E-50 | -1.69E-46 |
| MS.gene053449 | MS.gene06768 | 0.864623 | 1.06E-64 | -1.69E-46 |
| MS.gene053505 | MS.gene06768 | 0.842226 | 2.94E-58 | -1.69E-46 |
| MS.gene053574 | MS.gene06768 | 0.874935 | 4.58E-68 | -1.69E-46 |
| MS.gene053626 | MS.gene06768 | 0.864062 | 1.59E-64 | -1.69E-46 |
| MS.gene053824 | MS.gene06768 | 0.814678 | 1.36E-51 | -1.69E-46 |
| MS.gene053889 | MS.gene06768 | 0.802676 | 5.01E-49 | -1.69E-46 |
| MS.gene053912 | MS.gene06768 | 0.851837 | 6.84E-61 | -1.69E-46 |
| MS.gene053916 | MS.gene06768 | 0.85806 | 1.07E-62 | -1.69E-46 |
| MS.gene054003 | MS.gene06768 | 0.85451 | 1.17E-61 | -1.69E-46 |
| MS.gene054595 | MS.gene06768 | 0.813861 | 2.07E-51 | -1.69E-46 |
| MS.gene05494 | MS.gene06768 | 0.840677 | 7.53E-58 | -1.69E-46 |
| MS.gene05505 | MS.gene06768 | 0.841613 | 4.27E-58 | -1.69E-46 |
| MS.gene055224 | MS.gene06768 | 0.833774 | 4.40E-56 | -1.69E-46 |
| MS.gene055722 | MS.gene06768 | 0.832988 | 6.90E-56 | -1.69E-46 |
| MS.gene055741 | MS.gene06768 | 0.822872 | 1.88E-53 | -1.69E-46 |
| MS.gene055744 | MS.gene06768 | 0.822612 | 2.16E-53 | -1.69E-46 |
| MS.gene05589 | MS.gene06768 | 0.816672 | 4.90E-52 | -1.69E-46 |
| MS.gene055909 | MS.gene06768 | 0.838996 | 2.06E-57 | -1.69E-46 |
| MS.gene055911 | MS.gene06768 | 0.832536 | 8.94E-56 | -1.69E-46 |
| MS.gene05592 | MS.gene06768 | 0.813725 | 2.21E-51 | -1.69E-46 |
| MS.gene056046 | MS.gene06768 | 0.81049 | 1.12E-50 | -1.69E-46 |
| MS.gene056166 | MS.gene06768 | 0.854027 | 1.62E-61 | -1.69E-46 |
| MS.gene056387 | MS.gene06768 | 0.82587 | 3.70E-54 | -1.69E-46 |
| MS.gene056422 | MS.gene06768 | 0.914601 | 1.58E-84 | -1.69E-46 |
| MS.gene056446 | MS.gene06768 | 0.805171 | 1.52E-49 | -1.69E-46 |
| MS.gene056448 | MS.gene06768 | 0.839244 | 1.78E-57 | -1.69E-46 |
| MS.gene056487 | MS.gene06768 | 0.804967 | 1.67E-49 | -1.69E-46 |
| MS.gene056504 | MS.gene06768 | 0.829588 | 4.73E-55 | -1.69E-46 |
| MS.gene056511 | MS.gene06768 | 0.868463 | 6.41E-66 | -1.69E-46 |
| MS.gene05664 | MS.gene06768 | 0.804865 | 1.76E-49 | -1.69E-46 |
| MS.gene05666 | MS.gene06768 | 0.807342 | 5.30E-50 | -1.69E-46 |
| MS.gene057076 | MS.gene06768 | 0.893426 | 6.35E-75 | -1.69E-46 |
| MS.gene057086 | MS.gene06768 | 0.875073 | 4.11E-68 | -1.69E-46 |
| MS.gene057537 | MS.gene06768 | 0.805157 | 1.53E-49 | -1.69E-46 |
| MS.gene057734 | MS.gene06768 | 0.872344 | 3.42E-67 | -1.69E-46 |
| MS.gene057856 | MS.gene06768 | 0.801754 | 7.76E-49 | -1.69E-46 |
| MS.gene057876 | MS.gene06768 | 0.814791 | 1.29E-51 | -1.69E-46 |
| MS.gene05787 | MS.gene06768 | 0.818037 | 2.42E-52 | -1.69E-46 |
| MS.gene057926 | MS.gene06768 | 0.823669 | 1.22E-53 | -1.69E-46 |
| MS.gene058075 | MS.gene06768 | 0.832922 | 7.17E-56 | -1.69E-46 |
| MS.gene058120 | MS.gene06768 | 0.802587 | 5.23E-49 | -1.69E-46 |
| MS.gene058130 | MS.gene06768 | 0.841486 | 4.61E-58 | -1.69E-46 |
| MS.gene058419 | MS.gene06768 | 0.80297 | 4.36E-49 | -1.69E-46 |
| MS.gene058459 | MS.gene06768 | 0.856997 | 2.20E-62 | -1.69E-46 |
| MS.gene059037 | MS.gene06768 | -0.813683 | 2.26E-51 | -1.69E-46 |
| MS.gene05913 | MS.gene06768 | 0.876714 | 1.12E-68 | -1.69E-46 |
| MS.gene059305 | MS.gene06768 | 0.807086 | 6.00E-50 | -1.69E-46 |
| MS.gene059366 | MS.gene06768 | 0.816677 | 4.89E-52 | -1.69E-46 |
| MS.gene059367 | MS.gene06768 | 0.868575 | 5.90E-66 | -1.69E-46 |
| MS.gene059368 | MS.gene06768 | 0.871342 | 7.35E-67 | -1.69E-46 |
| MS.gene059455 | MS.gene06768 | 0.80875 | 2.66E-50 | -1.69E-46 |
| MS.gene059517 | MS.gene06768 | 0.816367 | 5.74E-52 | -1.69E-46 |
| MS.gene059667 | MS.gene06768 | 0.852344 | 4.91E-61 | -1.69E-46 |
| MS.gene059674 | MS.gene06768 | 0.829575 | 4.77E-55 | -1.69E-46 |
| MS.gene059680 | MS.gene06768 | 0.80615 | 9.46E-50 | -1.69E-46 |
| MS.gene059711 | MS.gene06768 | 0.842714 | 2.18E-58 | -1.69E-46 |
| MS.gene059807 | MS.gene06768 | 0.870617 | 1.27E-66 | -1.69E-46 |
| MS.gene059865 | MS.gene06768 | 0.8494 | 3.31E-60 | -1.69E-46 |
| MS.gene060372 | MS.gene06768 | 0.841244 | 5.34E-58 | -1.69E-46 |
| MS.gene060482 | MS.gene06768 | 0.841882 | 3.63E-58 | -1.69E-46 |
| MS.gene060778 | MS.gene06768 | 0.857293 | 1.80E-62 | -1.69E-46 |
| MS.gene060804 | MS.gene06768 | 0.822288 | 2.57E-53 | -1.69E-46 |
| MS.gene060835 | MS.gene06768 | 0.854845 | 9.38E-62 | -1.69E-46 |
| MS.gene060837 | MS.gene06768 | 0.854447 | 1.22E-61 | -1.69E-46 |
| MS.gene06097 | MS.gene06768 | 0.882485 | 1.00E-70 | -1.69E-46 |
| MS.gene060982 | MS.gene06768 | 0.831772 | 1.38E-55 | -1.69E-46 |
| MS.gene061041 | MS.gene06768 | 0.819244 | 1.29E-52 | -1.69E-46 |
| MS.gene061218 | MS.gene06768 | 0.8128 | 3.53E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06768.t1 | MTR_3g094080 | 99.415 | 171 | 1 | 0 | 1 | 171 | 1 | 171 | 5.64e-127 | 353 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06768.t1 | AT3G56290 | 60.920 | 174 | 62 | 2 | 1 | 170 | 1 | 172 | 5.05e-70 | 209 |
Find 30 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCTTCCTTCTTCTCTTGTT+TGG | 0.250102 | 3.3:+78283545 | None:intergenic |
| GCTAGAGAAGCCACAAAATC+TGG | 0.342436 | 3.3:-78283429 | MS.gene06768:CDS |
| CACTTGTCTTGCTTCACCCT+TGG | 0.361736 | 3.3:+78283034 | None:intergenic |
| AACATGCAAGGTATGCAAAA+AGG | 0.370388 | 3.3:-78283060 | MS.gene06768:CDS |
| TGTGCAGAGAAGGCAAAATC+TGG | 0.391867 | 3.3:-78282981 | MS.gene06768:CDS |
| GGAGCTCTTAGAAGGAAAAT+TGG | 0.394746 | 3.3:-78283288 | MS.gene06768:CDS |
| GAGAATATGTTGAAGAGGGT+TGG | 0.421247 | 3.3:-78283091 | MS.gene06768:CDS |
| AGAATATGTTGAAGAGGGTT+GGG | 0.437524 | 3.3:-78283090 | MS.gene06768:CDS |
| TGCAGTATGGAGCTCTTAGA+AGG | 0.445617 | 3.3:-78283296 | MS.gene06768:intron |
| TGAGCATCTTGTGCGGATCT+TGG | 0.446163 | 3.3:+78283465 | None:intergenic |
| AACATACATAAATGGGTCTT+GGG | 0.452482 | 3.3:-78283677 | None:intergenic |
| GAACATACATAAATGGGTCT+TGG | 0.457157 | 3.3:-78283678 | None:intergenic |
| TTCAGCCAAACAAGAGAAGA+AGG | 0.459242 | 3.3:-78283550 | MS.gene06768:CDS |
| GTATAATAAGGAGGGGTCAA+TGG | 0.496878 | 3.3:+78283648 | None:intergenic |
| GAAAATTGGTGGGACTTACA+AGG | 0.499480 | 3.3:-78283274 | MS.gene06768:CDS |
| GCAAGACAAGTGGACAAGCT+TGG | 0.505588 | 3.3:-78283023 | MS.gene06768:CDS |
| AATCTGGAGAACAAATGAGC+AGG | 0.519508 | 3.3:-78283413 | MS.gene06768:CDS |
| CTCTTAGAAGGAAAATTGGT+GGG | 0.533115 | 3.3:-78283284 | MS.gene06768:CDS |
| TGTTCATAAAGAGAAGAGTC+TGG | 0.537908 | 3.3:+78282947 | None:intergenic |
| AGAACAAATGAGCAGGGAGC+AGG | 0.543107 | 3.3:-78283406 | MS.gene06768:intron |
| GGATGGAGAATATGTTGAAG+AGG | 0.544093 | 3.3:-78283096 | MS.gene06768:CDS |
| TCATGTAGCATGTGCAGAGA+AGG | 0.566077 | 3.3:-78282991 | MS.gene06768:CDS |
| ATCTGGAGAACAAATGAGCA+GGG | 0.576834 | 3.3:-78283412 | MS.gene06768:CDS |
| AGATGCTCAGCTTATACAAG+AGG | 0.596458 | 3.3:-78283451 | MS.gene06768:CDS |
| GATGGAGAATATGTTGAAGA+GGG | 0.605246 | 3.3:-78283095 | MS.gene06768:CDS |
| GCTCTTAGAAGGAAAATTGG+TGG | 0.606049 | 3.3:-78283285 | MS.gene06768:CDS |
| AAAACTTGTATAATAAGGAG+GGG | 0.613562 | 3.3:+78283641 | None:intergenic |
| CAAGGGTGAAGCAAGACAAG+TGG | 0.622923 | 3.3:-78283033 | MS.gene06768:CDS |
| TTGGGTAGACAAAACATGCA+AGG | 0.654898 | 3.3:-78283072 | MS.gene06768:CDS |
| TATAAGCTGAGCATCTTGTG+CGG | 0.655921 | 3.3:+78283458 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAACTTGTATAATAAGGA+GGG | + | chr3.3:78282979-78282998 | None:intergenic | 20.0% |
| !! | GAAGAAAAACTTGTATAATA+AGG | + | chr3.3:78282983-78283002 | None:intergenic | 20.0% |
| !!! | AATTATAGGAGTATATTGTT+TGG | - | chr3.3:78283453-78283472 | MS.gene06768:CDS | 20.0% |
| !!! | TTTTTCAAATCTTATGTTGA+AGG | - | chr3.3:78283367-78283386 | MS.gene06768:intron | 20.0% |
| !!! | TTTTTGTAAATAATGCAGTA+TGG | - | chr3.3:78283307-78283326 | MS.gene06768:intron | 20.0% |
| ! | AAAACTTGTATAATAAGGAG+GGG | + | chr3.3:78282978-78282997 | None:intergenic | 25.0% |
| ! | GAAAAACTTGTATAATAAGG+AGG | + | chr3.3:78282980-78282999 | None:intergenic | 25.0% |
| !! | CATGTGATTAGTTGAATTAT+AGG | - | chr3.3:78283439-78283458 | MS.gene06768:CDS | 25.0% |
| ! | AGTTTTTCTTCAAAGTCATC+AGG | - | chr3.3:78282992-78283011 | MS.gene06768:CDS | 30.0% |
| AACATGCAAGGTATGCAAAA+AGG | - | chr3.3:78283556-78283575 | MS.gene06768:CDS | 35.0% | |
| AGAATATGTTGAAGAGGGTT+GGG | - | chr3.3:78283526-78283545 | MS.gene06768:CDS | 35.0% | |
| CTCTTAGAAGGAAAATTGGT+GGG | - | chr3.3:78283332-78283351 | MS.gene06768:intron | 35.0% | |
| GATGGAGAATATGTTGAAGA+GGG | - | chr3.3:78283521-78283540 | MS.gene06768:CDS | 35.0% | |
| TTCTTCCTTCTTCTCTTGTT+TGG | + | chr3.3:78283074-78283093 | None:intergenic | 35.0% | |
| ! | AATCTTGTTGTATTGAGCAG+TGG | - | chr3.3:78283499-78283518 | MS.gene06768:CDS | 35.0% |
| ! | CATTTGTTCTCCAGATTTTG+TGG | + | chr3.3:78283200-78283219 | None:intergenic | 35.0% |
| AATCTGGAGAACAAATGAGC+AGG | - | chr3.3:78283203-78283222 | MS.gene06768:intron | 40.0% | |
| AGATGCTCAGCTTATACAAG+AGG | - | chr3.3:78283165-78283184 | MS.gene06768:intron | 40.0% | |
| ATCTGGAGAACAAATGAGCA+GGG | - | chr3.3:78283204-78283223 | MS.gene06768:intron | 40.0% | |
| GAAAATTGGTGGGACTTACA+AGG | - | chr3.3:78283342-78283361 | MS.gene06768:intron | 40.0% | |
| GAGAATATGTTGAAGAGGGT+TGG | - | chr3.3:78283525-78283544 | MS.gene06768:CDS | 40.0% | |
| GCTCTTAGAAGGAAAATTGG+TGG | - | chr3.3:78283331-78283350 | MS.gene06768:intron | 40.0% | |
| GGAGCTCTTAGAAGGAAAAT+TGG | - | chr3.3:78283328-78283347 | MS.gene06768:intron | 40.0% | |
| GGATGGAGAATATGTTGAAG+AGG | - | chr3.3:78283520-78283539 | MS.gene06768:CDS | 40.0% | |
| GTATAATAAGGAGGGGTCAA+TGG | + | chr3.3:78282971-78282990 | None:intergenic | 40.0% | |
| GTATGCAAAAAGGACACCAA+GGG | - | chr3.3:78283566-78283585 | MS.gene06768:CDS | 40.0% | |
| TATAAGCTGAGCATCTTGTG+CGG | + | chr3.3:78283161-78283180 | None:intergenic | 40.0% | |
| TTCAGCCAAACAAGAGAAGA+AGG | - | chr3.3:78283066-78283085 | MS.gene06768:CDS | 40.0% | |
| TTGGGTAGACAAAACATGCA+AGG | - | chr3.3:78283544-78283563 | MS.gene06768:CDS | 40.0% | |
| TTGTTGTATTGAGCAGTGGA+TGG | - | chr3.3:78283503-78283522 | MS.gene06768:CDS | 40.0% | |
| GCTAGAGAAGCCACAAAATC+TGG | - | chr3.3:78283187-78283206 | MS.gene06768:intron | 45.0% | |
| GGTATGCAAAAAGGACACCA+AGG | - | chr3.3:78283565-78283584 | MS.gene06768:CDS | 45.0% | |
| TCATGTAGCATGTGCAGAGA+AGG | - | chr3.3:78283625-78283644 | MS.gene06768:CDS | 45.0% | |
| TGCAGTATGGAGCTCTTAGA+AGG | - | chr3.3:78283320-78283339 | MS.gene06768:intron | 45.0% | |
| TGTGCAGAGAAGGCAAAATC+TGG | - | chr3.3:78283635-78283654 | MS.gene06768:CDS | 45.0% | |
| AGAACAAATGAGCAGGGAGC+AGG | - | chr3.3:78283210-78283229 | MS.gene06768:intron | 50.0% | |
| CAAGGGTGAAGCAAGACAAG+TGG | - | chr3.3:78283583-78283602 | MS.gene06768:CDS | 50.0% | |
| CACTTGTCTTGCTTCACCCT+TGG | + | chr3.3:78283585-78283604 | None:intergenic | 50.0% | |
| GCAAGACAAGTGGACAAGCT+TGG | - | chr3.3:78283593-78283612 | MS.gene06768:CDS | 50.0% | |
| TGAGCATCTTGTGCGGATCT+TGG | + | chr3.3:78283154-78283173 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 78282950 | 78283688 | 78282950 | ID=MS.gene06768 |
| chr3.3 | mRNA | 78282950 | 78283688 | 78282950 | ID=MS.gene06768.t1;Parent=MS.gene06768 |
| chr3.3 | exon | 78283407 | 78283688 | 78283407 | ID=MS.gene06768.t1.exon1;Parent=MS.gene06768.t1 |
| chr3.3 | CDS | 78283407 | 78283688 | 78283407 | ID=cds.MS.gene06768.t1;Parent=MS.gene06768.t1 |
| chr3.3 | exon | 78283250 | 78283313 | 78283250 | ID=MS.gene06768.t1.exon2;Parent=MS.gene06768.t1 |
| chr3.3 | CDS | 78283250 | 78283313 | 78283250 | ID=cds.MS.gene06768.t1;Parent=MS.gene06768.t1 |
| chr3.3 | exon | 78282950 | 78283119 | 78282950 | ID=MS.gene06768.t1.exon3;Parent=MS.gene06768.t1 |
| chr3.3 | CDS | 78282950 | 78283119 | 78282950 | ID=cds.MS.gene06768.t1;Parent=MS.gene06768.t1 |
| Gene Sequence |
| Protein sequence |
