AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene26974


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26974.t1 MTR_1g022355 95.000 280 11 1 1 280 90 366 0.0 533
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene26974.t1 AT1G19520 55.755 278 104 4 1 274 108 370 1.78e-87 265
MS.gene26974.t1 AT1G19520 56.429 280 101 5 1 275 108 371 2.35e-84 267

Find 74 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAATTAAGTTCAAGATTTA+TGG 0.133676 1.3:-2624102 MS.gene26974:CDS
TCAGCCACTTGTTCCCATTT+AGG 0.207802 1.3:+2624063 None:intergenic
TTGATCTATCGGATCCTAAA+TGG 0.216797 1.3:-2624077 MS.gene26974:CDS
ATGAGGCGGGAGAAGTTATT+TGG 0.238365 1.3:-2624032 MS.gene26974:CDS
TTAGAGAGCTTAGGTTTAAA+TGG 0.238681 1.3:-2624308 MS.gene26974:CDS
GGAGGTTAGTAATAAGGTTT+TGG 0.281718 1.3:-2624442 MS.gene26974:CDS
TGTTTGCATTTCCCGGTTAT+CGG 0.314823 1.3:+2623994 None:intergenic
GGTAACTTGTTTGCATTTCC+CGG 0.333463 1.3:+2623987 None:intergenic
GAATTATCCTTTATACTTCA+AGG 0.334354 1.3:-2623836 MS.gene26974:CDS
AAGGAGGCGAAGCCGATAAC+CGG 0.340946 1.3:-2624006 MS.gene26974:CDS
TGTGTGGGAGGTTAGTAATA+AGG 0.359950 1.3:-2624448 MS.gene26974:CDS
TAGAGCTTCTACAGCCTACT+AGG 0.360591 1.3:-2623897 MS.gene26974:CDS
TGAAAGGGTTGTTTCAGAAA+TGG 0.373951 1.3:-2624559 MS.gene26974:CDS
TAAATGGGCACGTGAGAAAA+TGG 0.380143 1.3:-2624292 MS.gene26974:CDS
TGCATCTTCGCGATTTGATT+CGG 0.380839 1.3:-2624499 MS.gene26974:CDS
ATGGAAAAGGGTAGTGGTAG+TGG 0.392895 1.3:-2624540 MS.gene26974:CDS
ACAGTTACCTTGAAGTATAA+AGG 0.399658 1.3:+2623829 None:intergenic
TGTAGATTTGCTGGTGAAGT+TGG 0.408581 1.3:-2624348 MS.gene26974:CDS
GATGCTGGTGTTCGTGTTGA+TGG 0.410669 1.3:-2624171 MS.gene26974:CDS
AAATTAAGTTCAAGATTTAT+GGG 0.422169 1.3:-2624101 MS.gene26974:CDS
CGAGAAAATTCTGTCGTTGA+AGG 0.433246 1.3:-2623965 MS.gene26974:CDS
AAAGAGATGTGTAGATTTGC+TGG 0.447104 1.3:-2624357 MS.gene26974:CDS
GGTTGTTTCAGAAATGGAAA+AGG 0.448209 1.3:-2624553 MS.gene26974:CDS
GTTGACCCTGGTAGCTGAAT+GGG 0.449689 1.3:-2623920 MS.gene26974:CDS
ATGAGGATTTGGAGAAAATG+AGG 0.450985 1.3:-2624254 MS.gene26974:CDS
TAGAGAGCTTAGGTTTAAAT+GGG 0.451572 1.3:-2624307 MS.gene26974:CDS
TGATCTATCGGATCCTAAAT+GGG 0.452985 1.3:-2624076 MS.gene26974:CDS
GATTTATGGGCTTGATCTAT+CGG 0.456068 1.3:-2624088 MS.gene26974:CDS
TGGAAAAGGGTAGTGGTAGT+GGG 0.463563 1.3:-2624539 MS.gene26974:CDS
GTTGAGGAGAAGAAGGTTGT+TGG 0.471339 1.3:-2624144 MS.gene26974:CDS
TTGATCCAATCCACCCTAGT+AGG 0.474961 1.3:+2623883 None:intergenic
GCTCTACCCATTCAGCTACC+AGG 0.475118 1.3:+2623914 None:intergenic
TTGGTCTTCCTAAGAGGAAA+GGG 0.483400 1.3:-2624125 MS.gene26974:CDS
GCTGGTGAAGTTGGGATTCG+AGG 0.483854 1.3:-2624339 MS.gene26974:CDS
GTTGTTTCAGAAATGGAAAA+GGG 0.487089 1.3:-2624552 MS.gene26974:CDS
TGCTGCTTGTTATTGTTGAA+AGG 0.487258 1.3:-2624575 MS.gene26974:CDS
GAAAATGTTGTTGTTGATGC+TGG 0.487848 1.3:-2624186 MS.gene26974:CDS
AGGAAGAAGGAGAAAATGAA+AGG 0.488535 1.3:-2624402 MS.gene26974:CDS
AACAAGCAGCATTGCATCTA+CGG 0.491489 1.3:+2624587 None:intergenic
ATTGGATCAATTTGCTTGAA+AGG 0.495330 1.3:-2623870 MS.gene26974:CDS
TACAGCCTACTAGGGTGGAT+TGG 0.497105 1.3:-2623888 MS.gene26974:CDS
GTTATCGGCTTCGCCTCCTT+CGG 0.498408 1.3:+2624009 None:intergenic
GTAGATTTGCTGGTGAAGTT+GGG 0.499570 1.3:-2624347 MS.gene26974:CDS
GCGATTTGCTTAGAGAGCTT+AGG 0.500284 1.3:-2624317 MS.gene26974:CDS
TGTTGACCCTGGTAGCTGAA+TGG 0.506521 1.3:-2623921 MS.gene26974:CDS
GCTGCTTGTTATTGTTGAAA+GGG 0.513685 1.3:-2624574 MS.gene26974:CDS
GGAAGAAGGAGAAAATGAAA+GGG 0.516139 1.3:-2624401 MS.gene26974:CDS
TATGAATAAAGAGAGGAAGA+AGG 0.517752 1.3:-2624415 MS.gene26974:CDS
GGAAAAGGGTAGTGGTAGTG+GGG 0.518104 1.3:-2624538 MS.gene26974:CDS
AGGTTGTTGGTCTTCCTAAG+AGG 0.522381 1.3:-2624131 MS.gene26974:CDS
GAAAAGGGTAGTGGTAGTGG+GGG 0.524024 1.3:-2624537 MS.gene26974:CDS
TGGTAATGTTGAGGAGAAGA+AGG 0.529708 1.3:-2624151 MS.gene26974:CDS
AATTCTGTCGTTGAAGGAGA+AGG 0.546483 1.3:-2623959 MS.gene26974:CDS
GTTGGTCTTCCTAAGAGGAA+AGG 0.559694 1.3:-2624126 MS.gene26974:CDS
TCAGAAATGGAAAAGGGTAG+TGG 0.562055 1.3:-2624546 MS.gene26974:CDS
AGGAGGCGAAGCCGATAACC+GGG 0.567405 1.3:-2624005 MS.gene26974:CDS
AGAAGTTATTTGGCCGAAGG+AGG 0.576981 1.3:-2624022 MS.gene26974:CDS
GGCTGATAAGATTCATGAGG+CGG 0.580784 1.3:-2624046 MS.gene26974:CDS
GGGAGAAGTTATTTGGCCGA+AGG 0.586632 1.3:-2624025 MS.gene26974:CDS
GCTGATAAGATTCATGAGGC+GGG 0.589093 1.3:-2624045 MS.gene26974:CDS
TTTATGGAGGACTGTGTGGG+AGG 0.601402 1.3:-2624460 MS.gene26974:CDS
ATCTTCGCGATTTGATTCGG+TGG 0.603612 1.3:-2624496 MS.gene26974:CDS
AGGATTTATGGAGGACTGTG+TGG 0.605077 1.3:-2624464 MS.gene26974:CDS
CTGTCGTTGAAGGAGAAGGA+TGG 0.608593 1.3:-2623955 MS.gene26974:CDS
GGATCCTAAATGGGAACAAG+TGG 0.618929 1.3:-2624067 MS.gene26974:CDS
CTCTACCCATTCAGCTACCA+GGG 0.620249 1.3:+2623915 None:intergenic
GCTTCTACAGCCTACTAGGG+TGG 0.622270 1.3:-2623893 MS.gene26974:CDS
TCGTGTTGATGGTAATGTTG+AGG 0.627189 1.3:-2624160 MS.gene26974:CDS
AGAGCTTCTACAGCCTACTA+GGG 0.634855 1.3:-2623896 MS.gene26974:CDS
GGATTTATGGAGGACTGTGT+GGG 0.636168 1.3:-2624463 MS.gene26974:CDS
TGGTTGATATGAATAAAGAG+AGG 0.637788 1.3:-2624422 MS.gene26974:CDS
AGATGATAGTTTGTTGACCC+TGG 0.658807 1.3:-2623932 MS.gene26974:CDS
AGTGGCTGATAAGATTCATG+AGG 0.693084 1.3:-2624049 MS.gene26974:CDS
ATGGGCACGTGAGAAAATGG+AGG 0.695770 1.3:-2624289 MS.gene26974:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAAGTTCAAGATTTA+TGG - chr1.3:2624344-2624363 MS.gene26974:CDS 15.0%
!! AAATTAAGTTCAAGATTTAT+GGG - chr1.3:2624345-2624364 MS.gene26974:CDS 15.0%
! GAATTATCCTTTATACTTCA+AGG - chr1.3:2624610-2624629 MS.gene26974:CDS 25.0%
!! ATCAACAACAACATTTTCTT+CGG + chr1.3:2624258-2624277 None:intergenic 25.0%
ACAGTTACCTTGAAGTATAA+AGG + chr1.3:2624620-2624639 None:intergenic 30.0%
TAGAGAGCTTAGGTTTAAAT+GGG - chr1.3:2624139-2624158 MS.gene26974:CDS 30.0%
TATGAATAAAGAGAGGAAGA+AGG - chr1.3:2624031-2624050 MS.gene26974:CDS 30.0%
TTAGAGAGCTTAGGTTTAAA+TGG - chr1.3:2624138-2624157 MS.gene26974:CDS 30.0%
! ATTGGATCAATTTGCTTGAA+AGG - chr1.3:2624576-2624595 MS.gene26974:CDS 30.0%
! GTTGTTTCAGAAATGGAAAA+GGG - chr1.3:2623894-2623913 MS.gene26974:CDS 30.0%
! TACGGTACTTTTATCACATT+CGG + chr1.3:2623844-2623863 None:intergenic 30.0%
! TGGTTGATATGAATAAAGAG+AGG - chr1.3:2624024-2624043 MS.gene26974:CDS 30.0%
! TTAATTTTCCCTTTCCTCTT+AGG + chr1.3:2624332-2624351 None:intergenic 30.0%
!! ACATGAGTTTTATGAGGATT+TGG - chr1.3:2624181-2624200 MS.gene26974:CDS 30.0%
!! ATTTTAGTGAGGATTTATGG+AGG - chr1.3:2623973-2623992 MS.gene26974:CDS 30.0%
!! TGGATTTTAGTGAGGATTTA+TGG - chr1.3:2623970-2623989 MS.gene26974:CDS 30.0%
AAAGAGATGTGTAGATTTGC+TGG - chr1.3:2624089-2624108 MS.gene26974:CDS 35.0%
AGGAAGAAGGAGAAAATGAA+AGG - chr1.3:2624044-2624063 MS.gene26974:CDS 35.0%
ATGAGGATTTGGAGAAAATG+AGG - chr1.3:2624192-2624211 MS.gene26974:CDS 35.0%
GATTTATGGGCTTGATCTAT+CGG - chr1.3:2624358-2624377 MS.gene26974:CDS 35.0%
GGAAGAAGGAGAAAATGAAA+GGG - chr1.3:2624045-2624064 MS.gene26974:CDS 35.0%
TGATCTATCGGATCCTAAAT+GGG - chr1.3:2624370-2624389 MS.gene26974:CDS 35.0%
TTGATCTATCGGATCCTAAA+TGG - chr1.3:2624369-2624388 MS.gene26974:CDS 35.0%
! ACGGTACTTTTATCACATTC+GGG + chr1.3:2623843-2623862 None:intergenic 35.0%
! CTTCAACGACAGAATTTTCT+CGG + chr1.3:2624483-2624502 None:intergenic 35.0%
! GAAAATGTTGTTGTTGATGC+TGG - chr1.3:2624260-2624279 MS.gene26974:CDS 35.0%
! GGAGGTTAGTAATAAGGTTT+TGG - chr1.3:2624004-2624023 MS.gene26974:CDS 35.0%
! GGTTGTTTCAGAAATGGAAA+AGG - chr1.3:2623893-2623912 MS.gene26974:CDS 35.0%
! TGAAAGGGTTGTTTCAGAAA+TGG - chr1.3:2623887-2623906 MS.gene26974:CDS 35.0%
!! GCTGCTTGTTATTGTTGAAA+GGG - chr1.3:2623872-2623891 MS.gene26974:CDS 35.0%
!! TGCTGCTTGTTATTGTTGAA+AGG - chr1.3:2623871-2623890 MS.gene26974:CDS 35.0%
AACAAGCAGCATTGCATCTA+CGG + chr1.3:2623862-2623881 None:intergenic 40.0%
AATTCTGTCGTTGAAGGAGA+AGG - chr1.3:2624487-2624506 MS.gene26974:CDS 40.0%
AGTGGCTGATAAGATTCATG+AGG - chr1.3:2624397-2624416 MS.gene26974:CDS 40.0%
CGAGAAAATTCTGTCGTTGA+AGG - chr1.3:2624481-2624500 MS.gene26974:CDS 40.0%
TAAATGGGCACGTGAGAAAA+TGG - chr1.3:2624154-2624173 MS.gene26974:CDS 40.0%
TCAGAAATGGAAAAGGGTAG+TGG - chr1.3:2623900-2623919 MS.gene26974:CDS 40.0%
TGCATCTTCGCGATTTGATT+CGG - chr1.3:2623947-2623966 MS.gene26974:CDS 40.0%
TGTGTGGGAGGTTAGTAATA+AGG - chr1.3:2623998-2624017 MS.gene26974:CDS 40.0%
TGTTTGCATTTCCCGGTTAT+CGG + chr1.3:2624455-2624474 None:intergenic 40.0%
! CGGTACTTTTATCACATTCG+GGG + chr1.3:2623842-2623861 None:intergenic 40.0%
! TGGTAATGTTGAGGAGAAGA+AGG - chr1.3:2624295-2624314 MS.gene26974:CDS 40.0%
! TTGGTCTTCCTAAGAGGAAA+GGG - chr1.3:2624321-2624340 MS.gene26974:CDS 40.0%
!! AGATGATAGTTTGTTGACCC+TGG - chr1.3:2624514-2624533 MS.gene26974:CDS 40.0%
!! GGAGGAACATGAGTTTTATG+AGG - chr1.3:2624175-2624194 MS.gene26974:CDS 40.0%
!! GGTAACTTGTTTGCATTTCC+CGG + chr1.3:2624462-2624481 None:intergenic 40.0%
!! GTAGATTTGCTGGTGAAGTT+GGG - chr1.3:2624099-2624118 MS.gene26974:CDS 40.0%
!! TCGTGTTGATGGTAATGTTG+AGG - chr1.3:2624286-2624305 MS.gene26974:CDS 40.0%
!! TGTAGATTTGCTGGTGAAGT+TGG - chr1.3:2624098-2624117 MS.gene26974:CDS 40.0%
!!! TGATTCGGTGGATTTTAGTG+AGG - chr1.3:2623962-2623981 MS.gene26974:CDS 40.0%
AGAAGTTATTTGGCCGAAGG+AGG - chr1.3:2624424-2624443 MS.gene26974:CDS 45.0%
AGAGCTTCTACAGCCTACTA+GGG - chr1.3:2624550-2624569 MS.gene26974:CDS 45.0%
AGGATTTATGGAGGACTGTG+TGG - chr1.3:2623982-2624001 MS.gene26974:CDS 45.0%
ATGAGGCGGGAGAAGTTATT+TGG - chr1.3:2624414-2624433 MS.gene26974:CDS 45.0%
GCTGATAAGATTCATGAGGC+GGG - chr1.3:2624401-2624420 MS.gene26974:CDS 45.0%
GGATCCTAAATGGGAACAAG+TGG - chr1.3:2624379-2624398 MS.gene26974:CDS 45.0%
GGATTTATGGAGGACTGTGT+GGG - chr1.3:2623983-2624002 MS.gene26974:CDS 45.0%
GGCTGATAAGATTCATGAGG+CGG - chr1.3:2624400-2624419 MS.gene26974:CDS 45.0%
GTTGAGGAGAAGAAGGTTGT+TGG - chr1.3:2624302-2624321 MS.gene26974:CDS 45.0%
TAGAGCTTCTACAGCCTACT+AGG - chr1.3:2624549-2624568 MS.gene26974:CDS 45.0%
TCAGCCACTTGTTCCCATTT+AGG + chr1.3:2624386-2624405 None:intergenic 45.0%
TTGATCCAATCCACCCTAGT+AGG + chr1.3:2624566-2624585 None:intergenic 45.0%
! ATGGAAAAGGGTAGTGGTAG+TGG - chr1.3:2623906-2623925 MS.gene26974:CDS 45.0%
! GCGATTTGCTTAGAGAGCTT+AGG - chr1.3:2624129-2624148 MS.gene26974:CDS 45.0%
! TGGAAAAGGGTAGTGGTAGT+GGG - chr1.3:2623907-2623926 MS.gene26974:CDS 45.0%
!! AGGTTGTTGGTCTTCCTAAG+AGG - chr1.3:2624315-2624334 MS.gene26974:CDS 45.0%
!! ATCTTCGCGATTTGATTCGG+TGG - chr1.3:2623950-2623969 MS.gene26974:CDS 45.0%
!! GTTGGTCTTCCTAAGAGGAA+AGG - chr1.3:2624320-2624339 MS.gene26974:CDS 45.0%
ATGGGCACGTGAGAAAATGG+AGG - chr1.3:2624157-2624176 MS.gene26974:CDS 50.0%
CTCTACCCATTCAGCTACCA+GGG + chr1.3:2624534-2624553 None:intergenic 50.0%
CTGTCGTTGAAGGAGAAGGA+TGG - chr1.3:2624491-2624510 MS.gene26974:CDS 50.0%
GATGCTGGTGTTCGTGTTGA+TGG - chr1.3:2624275-2624294 MS.gene26974:CDS 50.0%
GGGAGAAGTTATTTGGCCGA+AGG - chr1.3:2624421-2624440 MS.gene26974:CDS 50.0%
GTTGACCCTGGTAGCTGAAT+GGG - chr1.3:2624526-2624545 MS.gene26974:CDS 50.0%
TACAGCCTACTAGGGTGGAT+TGG - chr1.3:2624558-2624577 MS.gene26974:CDS 50.0%
TGTTGACCCTGGTAGCTGAA+TGG - chr1.3:2624525-2624544 MS.gene26974:CDS 50.0%
TTTATGGAGGACTGTGTGGG+AGG - chr1.3:2623986-2624005 MS.gene26974:CDS 50.0%
! GGAAAAGGGTAGTGGTAGTG+GGG - chr1.3:2623908-2623927 MS.gene26974:CDS 50.0%
!! GAAAAGGGTAGTGGTAGTGG+GGG - chr1.3:2623909-2623928 MS.gene26974:CDS 50.0%
AAGGAGGCGAAGCCGATAAC+CGG - chr1.3:2624440-2624459 MS.gene26974:CDS 55.0%
GCTCTACCCATTCAGCTACC+AGG + chr1.3:2624535-2624554 None:intergenic 55.0%
GCTGGTGAAGTTGGGATTCG+AGG - chr1.3:2624107-2624126 MS.gene26974:CDS 55.0%
GCTTCTACAGCCTACTAGGG+TGG - chr1.3:2624553-2624572 MS.gene26974:CDS 55.0%
!! GTTATCGGCTTCGCCTCCTT+CGG + chr1.3:2624440-2624459 None:intergenic 55.0%
!! TAGTGGGGGTGGTGATGGAA+AGG - chr1.3:2623923-2623942 MS.gene26974:CDS 55.0%
AGGAGGCGAAGCCGATAACC+GGG - chr1.3:2624441-2624460 MS.gene26974:CDS 60.0%
!! AAGGGTAGTGGTAGTGGGGG+TGG - chr1.3:2623912-2623931 MS.gene26974:CDS 60.0%
!! AGTGGTAGTGGGGGTGGTGA+TGG - chr1.3:2623918-2623937 MS.gene26974:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 2623813 2624655 2623813 ID=MS.gene26974
chr1.3 mRNA 2623813 2624655 2623813 ID=MS.gene26974.t1;Parent=MS.gene26974
chr1.3 exon 2623813 2624655 2623813 ID=MS.gene26974.t1.exon1;Parent=MS.gene26974.t1
chr1.3 CDS 2623813 2624655 2623813 ID=cds.MS.gene26974.t1;Parent=MS.gene26974.t1
Gene Sequence

>MS.gene26974

ATGCGAAAAAAAGTTAAAGAAGTTTACCCCGAATGTGATAAAAGTACCGTAGATGCAATGCTGCTTGTTATTGTTGAAAGGGTTGTTTCAGAAATGGAAAAGGGTAGTGGTAGTGGGGGTGGTGATGGAAAGGTTGCATCTTCGCGATTTGATTCGGTGGATTTTAGTGAGGATTTATGGAGGACTGTGTGGGAGGTTAGTAATAAGGTTTTGGTTGATATGAATAAAGAGAGGAAGAAGGAGAAAATGAAAGGGTTTTTGCAGTGTGATGAAGTGAAAGAGATGTGTAGATTTGCTGGTGAAGTTGGGATTCGAGGCGATTTGCTTAGAGAGCTTAGGTTTAAATGGGCACGTGAGAAAATGGAGGAACATGAGTTTTATGAGGATTTGGAGAAAATGAGGAAAGAGACTCAGATTGTTGATGAAGAAATAAAAAATGAAACCGAAGAAAATGTTGTTGTTGATGCTGGTGTTCGTGTTGATGGTAATGTTGAGGAGAAGAAGGTTGTTGGTCTTCCTAAGAGGAAAGGGAAAATTAAGTTCAAGATTTATGGGCTTGATCTATCGGATCCTAAATGGGAACAAGTGGCTGATAAGATTCATGAGGCGGGAGAAGTTATTTGGCCGAAGGAGGCGAAGCCGATAACCGGGAAATGCAAACAAGTTACCGAGAAAATTCTGTCGTTGAAGGAGAAGGATGGAGATGATAGTTTGTTGACCCTGGTAGCTGAATGGGTAGAGCTTCTACAGCCTACTAGGGTGGATTGGATCAATTTGCTTGAAAGGTTGAAAAATCAGAATTATCCTTTATACTTCAAGGTAACTGTTGCATTGCCTTTTTAG

Protein sequence

>MS.gene26974.t1

MRKKVKEVYPECDKSTVDAMLLVIVERVVSEMEKGSGSGGGDGKVASSRFDSVDFSEDLWRTVWEVSNKVLVDMNKERKKEKMKGFLQCDEVKEMCRFAGEVGIRGDLLRELRFKWAREKMEEHEFYEDLEKMRKETQIVDEEIKNETEENVVVDAGVRVDGNVEEKKVVGLPKRKGKIKFKIYGLDLSDPKWEQVADKIHEAGEVIWPKEAKPITGKCKQVTEKILSLKEKDGDDSLLTLVAEWVELLQPTRVDWINLLERLKNQNYPLYFKVTVALPF