AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene34999

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34999.t1 MTR_7g067010 63.393 560 200 5 25 583 7 562 0.0 735
MS.gene34999.t1 MTR_3g462710 62.367 566 208 4 20 583 2 564 0.0 728
MS.gene34999.t1 MTR_4g015080 61.092 568 216 5 19 585 11 574 0.0 723
MS.gene34999.t1 MTR_3g462710 61.790 458 170 4 128 583 1 455 0.0 569
MS.gene34999.t1 MTR_7g098200 46.303 568 293 3 15 577 2 562 0.0 551
MS.gene34999.t1 MTR_7g098200 45.775 568 286 4 15 577 2 552 0.0 538
MS.gene34999.t1 MTR_7g098160 46.181 563 291 4 22 579 13 568 0.0 536
MS.gene34999.t1 MTR_7g098180 46.422 573 290 6 11 577 4 565 0.0 535
MS.gene34999.t1 MTR_7g098040 45.391 575 300 5 10 579 4 569 0.0 525
MS.gene34999.t1 MTR_7g098090 45.250 579 298 6 11 579 4 573 3.25e-180 522
MS.gene34999.t1 MTR_1g075440 45.745 564 290 5 11 569 1 553 4.15e-180 521
MS.gene34999.t1 MTR_3g112460 46.786 560 283 5 28 585 9 555 3.13e-177 513
MS.gene34999.t1 MTR_1g042310 44.050 563 307 3 20 579 4 561 1.80e-175 509
MS.gene34999.t1 MTR_7g098220 45.709 571 298 4 14 579 2 565 3.50e-175 509
MS.gene34999.t1 MTR_3g112470 46.607 560 284 5 28 585 9 555 1.53e-174 506
MS.gene34999.t1 MTR_1g041835 44.186 559 303 2 24 579 9 561 2.00e-172 501
MS.gene34999.t1 MTR_1g041815 44.544 559 301 2 24 579 9 561 2.46e-172 502
MS.gene34999.t1 MTR_3g072300 44.190 568 310 4 20 585 2 564 1.12e-170 497
MS.gene34999.t1 MTR_7g098230 44.485 544 290 4 39 577 23 559 2.20e-169 494
MS.gene34999.t1 MTR_1g041875 43.911 542 289 3 24 562 11 540 6.57e-169 493
MS.gene34999.t1 MTR_5g055000 45.073 548 295 5 40 583 16 561 4.21e-168 491
MS.gene34999.t1 MTR_3g112450 45.989 561 286 6 28 584 9 556 6.13e-167 487
MS.gene34999.t1 MTR_5g014150 43.728 574 316 5 12 580 15 586 7.36e-167 488
MS.gene34999.t1 MTR_4g098800 43.034 567 312 4 20 581 14 574 5.09e-163 478
MS.gene34999.t1 MTR_4g098800 42.882 576 315 5 14 581 70 639 2.28e-162 478
MS.gene34999.t1 MTR_7g010820 44.248 565 306 5 26 583 10 572 1.06e-160 472
MS.gene34999.t1 MTR_1g042310 43.893 524 286 3 59 579 6 524 3.94e-160 469
MS.gene34999.t1 MTR_1g116930 42.629 563 312 6 20 580 2 555 9.95e-160 469
MS.gene34999.t1 MTR_7g005930 42.989 542 299 5 41 580 34 567 4.59e-159 468
MS.gene34999.t1 MTR_3g069420 42.632 570 312 8 18 582 10 569 6.40e-159 468
MS.gene34999.t1 MTR_3g005010 44.621 567 273 7 26 585 14 546 1.62e-158 465
MS.gene34999.t1 MTR_1g042310 43.678 522 286 3 61 579 1 517 1.83e-158 464
MS.gene34999.t1 MTR_7g098220 45.627 526 274 4 59 579 3 521 7.02e-158 463
MS.gene34999.t1 MTR_7g088825 41.476 569 322 8 20 582 2 565 3.61e-157 463
MS.gene34999.t1 MTR_3g069500 41.901 568 309 7 18 582 11 560 9.35e-156 459
MS.gene34999.t1 MTR_7g088790 41.607 560 320 6 28 582 15 572 2.72e-155 459
MS.gene34999.t1 MTR_7g013270 42.032 571 316 7 15 580 64 624 7.13e-155 459
MS.gene34999.t1 MTR_4g033325 44.065 556 281 8 28 579 17 546 1.14e-154 455
MS.gene34999.t1 MTR_5g012270 42.637 584 315 10 14 585 2 577 1.19e-154 456
MS.gene34999.t1 MTR_1g041885 43.307 508 273 3 61 565 1 496 2.52e-154 454
MS.gene34999.t1 MTR_7g088820 41.316 547 312 7 31 572 16 558 5.38e-154 455
MS.gene34999.t1 MTR_5g012290 43.525 556 307 4 32 582 17 570 4.50e-153 452
MS.gene34999.t1 MTR_0294s0020 43.958 571 287 9 13 579 5 546 6.04e-152 448
MS.gene34999.t1 MTR_7g065080 41.812 574 317 8 13 584 2 560 3.96e-151 447
MS.gene34999.t1 MTR_3g069440 42.576 559 308 7 28 582 19 568 5.89e-150 444
MS.gene34999.t1 MTR_4g019580 41.343 566 323 6 20 584 14 571 2.17e-149 443
MS.gene34999.t1 MTR_4g033315 42.626 556 291 7 28 579 17 548 1.71e-148 439
MS.gene34999.t1 MTR_4g033310 42.626 556 291 7 28 579 17 548 1.71e-148 439
MS.gene34999.t1 MTR_7g098150 46.374 455 232 4 22 471 13 460 2.29e-147 434
MS.gene34999.t1 MTR_4g101380 42.832 572 313 7 21 582 6 573 3.39e-146 435
MS.gene34999.t1 MTR_4g033330 43.438 541 278 8 28 564 17 533 1.11e-144 429
MS.gene34999.t1 MTR_4g114340 43.058 569 309 6 20 583 2 560 2.02e-142 424
MS.gene34999.t1 MTR_7g088820 41.903 494 280 6 84 572 5 496 2.91e-140 417
MS.gene34999.t1 MTR_5g085850 41.093 567 310 4 21 582 9 556 6.56e-140 419
MS.gene34999.t1 MTR_7g006540 39.249 586 332 9 17 582 31 612 6.15e-139 418
MS.gene34999.t1 MTR_8g038880 42.259 549 289 8 44 583 22 551 8.85e-138 412
MS.gene34999.t1 MTR_5g038060 40.868 553 313 7 40 582 29 577 2.02e-136 410
MS.gene34999.t1 MTR_7g098200 43.516 455 245 3 128 577 1 448 1.81e-135 404
MS.gene34999.t1 MTR_4g033395 42.460 504 263 9 28 527 18 498 6.48e-135 402
MS.gene34999.t1 MTR_3g014790 40.422 569 301 10 28 583 5 548 9.21e-135 404
MS.gene34999.t1 MTR_4g033375 42.970 505 259 9 28 527 18 498 1.81e-134 402
MS.gene34999.t1 MTR_4g107510 35.846 597 359 8 1 584 1 586 1.96e-131 397
MS.gene34999.t1 MTR_2g017750 38.898 581 339 9 17 582 9 588 2.45e-131 397
MS.gene34999.t1 MTR_4g107670 37.842 584 332 8 20 584 2 573 7.77e-131 395
MS.gene34999.t1 MTR_8g087780 39.613 568 330 7 28 582 7 574 1.85e-130 394
MS.gene34999.t1 MTR_8g469480 38.686 548 320 8 46 582 42 584 4.55e-129 391
MS.gene34999.t1 MTR_4g107530 37.838 592 340 9 4 583 7 582 7.48e-129 390
MS.gene34999.t1 MTR_8g469470 36.177 586 349 7 13 585 11 584 2.06e-128 389
MS.gene34999.t1 MTR_2g005130 39.677 557 309 8 28 579 26 560 3.38e-127 386
MS.gene34999.t1 MTR_3g100980 37.906 554 324 5 36 582 10 550 5.29e-126 382
MS.gene34999.t1 MTR_4g107580 35.235 596 349 6 1 582 1 573 2.03e-125 382
MS.gene34999.t1 MTR_7g088820 41.333 450 257 6 128 572 1 448 2.95e-124 375
MS.gene34999.t1 MTR_8g103233 39.234 548 318 8 39 579 14 553 3.10e-123 375
MS.gene34999.t1 MTR_8g469310 37.913 575 342 8 17 582 3 571 5.35e-123 375
MS.gene34999.t1 MTR_7g065080 41.478 487 268 8 100 584 2 473 2.30e-122 370
MS.gene34999.t1 MTR_5g038380 37.435 577 343 8 17 582 5 574 1.04e-121 372
MS.gene34999.t1 MTR_3g100980 36.254 582 323 6 36 582 10 578 8.61e-121 370
MS.gene34999.t1 MTR_4g033325 42.451 457 233 8 127 579 18 448 7.74e-119 360
MS.gene34999.t1 MTR_8g087810 39.442 573 325 8 28 582 7 575 9.56e-119 364
MS.gene34999.t1 MTR_5g038190 35.103 584 345 8 10 582 8 568 2.75e-117 360
MS.gene34999.t1 MTR_4g033325 42.661 436 229 6 148 579 4 422 2.26e-116 353
MS.gene34999.t1 MTR_1g085090 35.472 561 341 7 27 582 2 546 3.59e-116 358
MS.gene34999.t1 MTR_4g033385 40.919 457 243 9 127 579 16 449 5.81e-115 350
MS.gene34999.t1 MTR_7g111260 35.897 546 331 6 40 582 17 546 1.33e-114 353
MS.gene34999.t1 MTR_1g026740 36.396 555 336 7 34 579 7 553 3.01e-112 347
MS.gene34999.t1 MTR_2g101650 35.417 576 338 8 13 579 13 563 3.20e-112 347
MS.gene34999.t1 MTR_2g101590 35.690 580 332 11 12 579 15 565 4.73e-112 348
MS.gene34999.t1 MTR_1g009200 36.996 546 324 8 44 579 29 564 2.19e-111 345
MS.gene34999.t1 MTR_4g123537 37.410 556 329 7 32 583 31 571 2.87e-111 345
MS.gene34999.t1 MTR_1g026750 35.702 563 343 6 31 583 5 558 5.08e-111 343
MS.gene34999.t1 MTR_2g101610 36.413 552 329 10 40 579 25 566 2.64e-110 344
MS.gene34999.t1 MTR_4g024930 36.077 571 328 10 18 580 12 553 1.23e-107 335
MS.gene34999.t1 MTR_2g101640 34.715 579 345 9 9 579 9 562 2.49e-105 331
MS.gene34999.t1 MTR_7g098210 37.285 523 236 11 61 577 1 437 1.81e-104 322
MS.gene34999.t1 MTR_4g107610 34.938 561 337 10 41 583 30 580 3.33e-104 327
MS.gene34999.t1 MTR_1g026770 35.584 548 337 8 44 584 17 555 1.69e-103 324
MS.gene34999.t1 MTR_4g011280 34.552 547 342 8 41 579 31 569 2.55e-102 322
MS.gene34999.t1 MTR_7g064780 32.049 571 376 7 17 579 1 567 2.90e-100 316
MS.gene34999.t1 MTR_2g101560 34.177 553 340 10 40 579 25 566 1.25e-99 316
MS.gene34999.t1 MTR_1g057460 34.555 573 329 17 20 579 25 564 3.96e-99 313
MS.gene34999.t1 MTR_6g008690 33.808 562 315 11 47 585 12 539 1.86e-93 297
MS.gene34999.t1 MTR_1g057460 35.409 514 291 14 77 579 10 493 3.62e-92 292
MS.gene34999.t1 MTR_7g064800 33.942 548 348 8 40 577 24 567 4.95e-91 292
MS.gene34999.t1 MTR_0236s0050 34.517 507 305 10 77 579 10 493 1.33e-86 278
MS.gene34999.t1 MTR_2g093640 30.182 550 344 9 44 580 23 545 1.42e-75 250
MS.gene34999.t1 MTR_4g033310 43.478 276 147 3 306 579 216 484 1.60e-75 249
MS.gene34999.t1 MTR_4g033310 48.901 182 84 2 28 209 17 189 4.95e-46 169
MS.gene34999.t1 MTR_2g093160 31.193 545 345 10 44 580 23 545 1.82e-73 245
MS.gene34999.t1 MTR_2g093180 30.702 570 353 11 28 580 2 546 6.80e-72 241
MS.gene34999.t1 MTR_7g052600 29.663 563 355 9 19 579 2 525 7.15e-72 240
MS.gene34999.t1 MTR_7g051880 29.783 554 348 9 28 579 11 525 4.46e-71 238
MS.gene34999.t1 MTR_7g051830 29.422 554 350 8 28 579 11 525 8.30e-70 234
MS.gene34999.t1 MTR_1g009170 32.506 403 210 11 167 561 9 357 3.85e-56 194
MS.gene34999.t1 MTR_3g065350 39.634 164 93 1 351 514 1 158 6.90e-30 115
MS.gene34999.t1 MTR_4g033390 46.988 83 44 0 497 579 30 112 1.61e-21 90.9
MS.gene34999.t1 MTR_8g091265 37.069 116 69 4 312 423 3 118 1.12e-12 66.2
MS.gene34999.t1 MTR_4g124330 31.405 121 60 2 144 264 8 105 2.36e-11 61.6
MS.gene34999.t1 MTR_7g064160 27.500 120 81 1 17 136 4 117 3.88e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene34999.t1 AT2G02040 73.846 585 152 1 1 585 1 584 0.0 912
MS.gene34999.t1 AT1G62200 63.087 596 200 4 1 584 2 589 0.0 772
MS.gene34999.t1 AT1G62200 60.320 625 199 5 1 584 16 632 0.0 758
MS.gene34999.t1 AT1G62200 60.320 625 199 5 1 584 2 618 0.0 757
MS.gene34999.t1 AT3G54140 62.544 566 209 3 20 584 2 565 0.0 743
MS.gene34999.t1 AT3G54140 62.544 566 209 3 20 584 2 565 0.0 743
MS.gene34999.t1 AT5G01180 59.859 568 224 4 19 585 2 566 0.0 721
MS.gene34999.t1 AT5G01180 59.859 568 224 4 19 585 2 566 0.0 721
MS.gene34999.t1 AT5G01180 59.859 568 224 4 19 585 2 566 0.0 721
MS.gene34999.t1 AT2G02020 60.751 586 187 7 1 584 1 545 0.0 716
MS.gene34999.t1 AT2G02020 57.338 586 186 8 1 584 1 524 0.0 657
MS.gene34999.t1 AT5G46050 44.444 549 292 5 19 562 4 544 8.54e-168 490
MS.gene34999.t1 AT1G32450 42.660 579 321 7 11 580 6 582 7.07e-165 483
MS.gene34999.t1 AT1G68570 45.167 569 305 6 19 582 2 568 4.83e-163 478
MS.gene34999.t1 AT2G40460 43.186 565 308 4 20 582 2 555 3.80e-162 475
MS.gene34999.t1 AT5G46040 43.278 543 295 5 25 562 10 544 2.10e-158 466
MS.gene34999.t1 AT1G68570 46.442 534 281 5 54 582 5 538 2.18e-158 465
MS.gene34999.t1 AT4G21680 42.660 579 314 8 19 584 2 575 5.64e-155 457
MS.gene34999.t1 AT2G26690 43.937 569 303 7 20 583 2 559 9.73e-153 451
MS.gene34999.t1 AT3G53960 40.964 581 331 6 5 582 2 573 6.27e-152 450
MS.gene34999.t1 AT2G37900 39.826 575 330 6 15 585 9 571 1.98e-147 437
MS.gene34999.t1 AT5G19640 40.522 575 319 7 11 580 26 582 2.49e-146 436
MS.gene34999.t1 AT1G12110 42.857 560 308 5 32 583 17 572 1.16e-145 433
MS.gene34999.t1 AT1G22540 42.321 560 293 9 30 583 18 553 2.35e-143 426
MS.gene34999.t1 AT2G26690 44.299 535 282 7 54 583 3 526 4.74e-143 425
MS.gene34999.t1 AT3G54450 39.564 551 309 7 40 583 10 543 7.41e-142 422
MS.gene34999.t1 AT2G26690 44.275 524 276 7 65 583 2 514 2.00e-139 416
MS.gene34999.t1 AT1G69870 39.437 568 329 8 23 582 34 594 2.88e-138 416
MS.gene34999.t1 AT1G72140 41.429 560 286 8 30 579 22 549 1.10e-137 412
MS.gene34999.t1 AT5G13400 39.108 583 330 10 19 582 35 611 1.76e-135 408
MS.gene34999.t1 AT1G22570 42.755 559 284 11 12 560 6 538 9.80e-135 404
MS.gene34999.t1 AT1G59740 39.304 575 331 8 6 573 5 568 7.41e-134 403
MS.gene34999.t1 AT1G22550 39.243 581 318 9 12 583 6 560 1.69e-130 394
MS.gene34999.t1 AT3G21670 39.753 566 311 6 28 582 12 558 9.69e-128 387
MS.gene34999.t1 AT1G69850 37.024 578 348 6 19 582 4 579 1.79e-127 387
MS.gene34999.t1 AT1G72130 39.318 557 297 9 34 585 16 536 5.79e-127 384
MS.gene34999.t1 AT2G37900 39.563 503 290 5 85 585 2 492 1.09e-126 381
MS.gene34999.t1 AT1G33440 39.216 561 315 8 28 579 24 567 4.24e-126 384
MS.gene34999.t1 AT1G27040 37.566 567 320 5 28 580 9 555 9.19e-126 381
MS.gene34999.t1 AT1G27040 37.566 567 320 5 28 580 13 559 1.34e-125 381
MS.gene34999.t1 AT1G52190 38.087 554 323 8 40 579 19 566 1.63e-125 382
MS.gene34999.t1 AT1G22570 42.857 518 266 9 53 560 4 501 2.62e-125 379
MS.gene34999.t1 AT3G16180 39.318 557 318 10 36 579 16 565 5.89e-125 380
MS.gene34999.t1 AT1G72125 39.965 573 314 11 17 583 9 557 1.22e-124 379
MS.gene34999.t1 AT5G46050 42.955 440 238 5 128 562 1 432 1.44e-123 372
MS.gene34999.t1 AT1G27040 38.219 539 299 5 56 580 9 527 2.88e-121 369
MS.gene34999.t1 AT1G18880 37.705 549 325 9 46 582 23 566 4.37e-121 370
MS.gene34999.t1 AT1G72120 39.415 581 308 12 14 583 6 553 9.84e-120 365
MS.gene34999.t1 AT1G27080 36.057 563 333 10 34 585 5 551 9.24e-112 346
MS.gene34999.t1 AT5G62730 34.596 581 331 6 18 579 32 582 2.32e-108 337
MS.gene34999.t1 AT5G62680 36.116 551 333 10 46 582 47 592 4.99e-107 335
MS.gene34999.t1 AT3G47960 35.101 547 342 7 46 582 65 608 4.41e-103 325
MS.gene34999.t1 AT1G69860 37.179 546 322 7 46 585 22 552 1.16e-102 322
MS.gene34999.t1 AT5G14940 34.103 563 325 10 40 582 5 541 3.89e-102 320
MS.gene34999.t1 AT5G28470 32.486 551 357 8 36 579 11 553 4.04e-102 320
MS.gene34999.t1 AT5G62730 34.664 551 311 6 48 579 3 523 4.23e-102 319
MS.gene34999.t1 AT1G27080 36.138 523 308 9 73 585 3 509 2.90e-100 315
MS.gene34999.t1 AT5G28470 32.305 551 356 9 36 579 11 551 3.80e-99 312
MS.gene34999.t1 AT1G72130 39.261 433 231 7 157 585 14 418 6.02e-99 307
MS.gene34999.t1 AT1G72130 39.261 433 231 7 157 585 14 418 6.02e-99 307
MS.gene34999.t1 AT3G01350 32.504 563 343 12 39 580 3 549 9.45e-91 291
MS.gene34999.t1 AT3G25260 31.746 567 331 14 17 579 1 515 3.40e-86 277
MS.gene34999.t1 AT3G45650 29.381 565 366 7 26 583 6 544 3.07e-83 271
MS.gene34999.t1 AT3G45680 29.982 567 359 12 26 583 6 543 6.31e-80 262
MS.gene34999.t1 AT3G45650 28.257 591 365 8 26 583 6 570 2.35e-78 259
MS.gene34999.t1 AT3G45660 30.297 538 348 7 50 583 29 543 5.25e-78 257
MS.gene34999.t1 AT3G45660 30.297 538 348 7 50 583 29 543 5.25e-78 257
MS.gene34999.t1 AT3G45660 29.930 568 368 8 23 583 20 564 5.70e-78 258
MS.gene34999.t1 AT3G25280 30.769 559 328 10 32 579 11 521 3.48e-77 254
MS.gene34999.t1 AT3G45720 28.889 540 353 10 50 583 27 541 2.25e-73 244
MS.gene34999.t1 AT3G45710 28.804 552 358 11 41 583 20 545 4.92e-73 244
MS.gene34999.t1 AT3G45700 27.941 544 360 10 44 583 18 533 5.55e-69 233
MS.gene34999.t1 AT3G45690 27.422 547 326 13 30 568 4 487 1.35e-60 209
MS.gene34999.t1 AT5G11570 35.769 260 155 4 324 581 229 478 3.11e-41 156
MS.gene34999.t1 AT5G11570 34.177 237 144 6 28 259 2 231 8.43e-34 135
MS.gene34999.t1 AT2G38100 27.360 519 332 14 70 579 2 484 1.62e-40 154
MS.gene34999.t1 AT2G38100 27.360 519 332 14 70 579 18 500 3.05e-40 154
MS.gene34999.t1 AT2G38100 27.360 519 332 14 70 579 24 506 3.42e-40 154

Find 141 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGCATTCCTACTTTATTTA+TGG 0.075098 4.4:-18351287 MS.gene34999:CDS
ATACCTCAGTATTTCTTATT+TGG 0.084877 4.4:-18350399 MS.gene34999:CDS
CAATATGCTGCCTTCTTCTT+TGG 0.186574 4.4:-18351517 MS.gene34999:CDS
TGATCCGAATATTTCCAATT+TGG 0.187016 4.4:-18350785 MS.gene34999:CDS
AAACCATGTGTGTCGTCTTT+TGG 0.206810 4.4:-18351460 MS.gene34999:CDS
TACCTCAGTATTTCTTATTT+GGG 0.227659 4.4:-18350398 MS.gene34999:CDS
ACTTGGAACATTCTTGTAAT+AGG 0.247470 4.4:+18351199 None:intergenic
TGACCTTTGTTCAGGTTATC+TGG 0.248081 4.4:+18350192 None:intergenic
GGCATTCCTACTTTATTTAT+GGG 0.268231 4.4:-18351286 MS.gene34999:CDS
GGTCTCTACCTGATTGCATT+TGG 0.272617 4.4:-18351496 MS.gene34999:CDS
AAAAGCTTACCTAGGATAAA+TGG 0.279478 4.4:+18352506 None:intergenic
AATAATTACAATGAAATAAA+TGG 0.281390 4.4:+18352193 None:intergenic
ACTATTCCGGCAGCCCAAAT+TGG 0.290011 4.4:+18350771 None:intergenic
ACCCCTTCTTAACCCTTTCT+TGG 0.293483 4.4:+18351410 None:intergenic
TGCTAAATATTGAGATAAAA+AGG 0.294191 4.4:+18350523 None:intergenic
GTCTCTACCTGATTGCATTT+GGG 0.300996 4.4:-18351495 MS.gene34999:CDS
CCCCTTCTTAACCCTTTCTT+GGG 0.315339 4.4:+18351411 None:intergenic
GATCCGAATATTTCCAATTT+GGG 0.320698 4.4:-18350784 MS.gene34999:CDS
CAGCAACAGGTTTATCAATA+AGG 0.341199 4.4:+18350445 None:intergenic
GACAAGAGTTCTGCAATTCT+AGG 0.341415 4.4:-18351106 MS.gene34999:CDS
CCCCTGGCAAGGAAATTTAC+AGG 0.345067 4.4:-18350588 MS.gene34999:CDS
AATCAAGATGACCTTTGTTC+AGG 0.347116 4.4:+18350184 None:intergenic
TGCCTGTAAATTTCCTTGCC+AGG 0.348055 4.4:+18350586 None:intergenic
GAAAATGCAGGCTGGGGTAT+TGG 0.355845 4.4:-18351313 MS.gene34999:CDS
AACATAGGAGCCCTTGTATC+AGG 0.359748 4.4:-18351358 MS.gene34999:CDS
ATTCTTACTTGGGGCGATAC+TGG 0.361479 4.4:-18352235 MS.gene34999:CDS
TTGAAGCCTGCAGACTGTTT+CGG 0.363256 4.4:-18351568 MS.gene34999:CDS
TGCGAACGTTTGGCATACTA+TGG 0.375818 4.4:-18352386 MS.gene34999:CDS
ATACTAATTGGTACAGCAAC+AGG 0.384112 4.4:+18350432 None:intergenic
TATTCTAGCTGATTCTTACT+TGG 0.386592 4.4:-18352246 MS.gene34999:CDS
CATAAACTCCGCATGGCGTC+TGG 0.388446 4.4:+18350321 None:intergenic
GGAAGAAGAATCTCTTCTTC+AGG 0.394408 4.4:-18352882 MS.gene34999:intron
CAGCCCAAATTGGAAATATT+CGG 0.398388 4.4:+18350781 None:intergenic
CAATGAATGTTGCGAACGTT+TGG 0.402936 4.4:-18352396 MS.gene34999:CDS
TTATTTATGGGATTAGCTAT+AGG 0.403195 4.4:-18351274 MS.gene34999:CDS
ACTACACAAGGTGGAAATCC+TGG 0.403776 4.4:-18350222 MS.gene34999:CDS
TGCTATCTTACACCACTCAT+TGG 0.408107 4.4:-18352272 MS.gene34999:CDS
GATGGTTCGGTTGACTTTGA+AGG 0.415640 4.4:-18352572 MS.gene34999:CDS
ATTCTAGCTGATTCTTACTT+GGG 0.418870 4.4:-18352245 MS.gene34999:CDS
GCGGAGTTTATGCAGTGCTT+TGG 0.423325 4.4:-18350310 MS.gene34999:CDS
CCACAAACATTGTTGACAGC+TGG 0.430858 4.4:+18350730 None:intergenic
ATGTAGTTTGCGGGTGAGAT+AGG 0.443137 4.4:+18352343 None:intergenic
TCCGCATGGCGTCTGGAGAT+TGG 0.446869 4.4:+18350328 None:intergenic
CCGGTATTCTGCTTGAGAAC+AGG 0.450038 4.4:+18352545 None:intergenic
CACCAGTCCCAAATGCAATC+AGG 0.453567 4.4:+18351488 None:intergenic
TACCTGATTGCATTTGGGAC+TGG 0.455757 4.4:-18351490 MS.gene34999:CDS
CATTTCCTTGATGTAGTTTG+CGG 0.458161 4.4:+18352333 None:intergenic
GCAGGCTGGGGTATTGGATT+CGG 0.460754 4.4:-18351307 MS.gene34999:CDS
TACATTGACTCTTTCTGCAT+CGG 0.462493 4.4:-18351599 MS.gene34999:CDS
ATTTCCTTGATGTAGTTTGC+GGG 0.466534 4.4:+18352334 None:intergenic
CAATAAAAGTGCCTGATACA+AGG 0.470243 4.4:+18351347 None:intergenic
GCGATACACCGGAGATGGTT+CGG 0.475150 4.4:-18352585 MS.gene34999:CDS
ATGATACTGATCCCCAAGAA+AGG 0.475732 4.4:-18351423 MS.gene34999:CDS
ACTTAGCTTCTTAAATATGT+TGG 0.477213 4.4:-18350139 MS.gene34999:CDS
GAGTTGCAAAGAATGGGAAT+TGG 0.483571 4.4:-18350546 MS.gene34999:CDS
GCCTGTAAATTTCCTTGCCA+GGG 0.484042 4.4:+18350587 None:intergenic
AAAGCTTGTCCATTTATCCT+AGG 0.484795 4.4:-18352515 MS.gene34999:intron
TCTGGGTTCCAGTCTATGAC+AGG 0.485500 4.4:-18350620 MS.gene34999:CDS
AGAATTGCAGAACTCTTGTC+TGG 0.490797 4.4:+18351109 None:intergenic
TCAAAACTTGAGAGGGAAGC+TGG 0.492545 4.4:+18350660 None:intergenic
TACAAGAATGTTCCAAGTTG+TGG 0.492885 4.4:-18351194 MS.gene34999:CDS
GAAAAGATGCTACCACAACT+TGG 0.493115 4.4:+18351182 None:intergenic
AAAGTCAACCGAACCATCTC+CGG 0.494164 4.4:+18352577 None:intergenic
TCCCCAAGAAAGGGTTAAGA+AGG 0.498271 4.4:-18351413 MS.gene34999:CDS
CACAAACATTGTTGACAGCT+GGG 0.503033 4.4:+18350731 None:intergenic
ATATTTCCAATTTGGGCTGC+CGG 0.503529 4.4:-18350777 MS.gene34999:CDS
TACAAGAGACTACTATCCTC+AGG 0.507492 4.4:+18351139 None:intergenic
ATGGTTCGGTTGACTTTGAA+GGG 0.508855 4.4:-18352571 MS.gene34999:CDS
GATTCAAGAAAATGCAGGCT+GGG 0.519096 4.4:-18351320 MS.gene34999:CDS
ACTATAATCCTGTCATAGAC+TGG 0.521555 4.4:+18350612 None:intergenic
TTCTGAAGGATGAGCAGAGA+AGG 0.522440 4.4:-18352607 MS.gene34999:intron
CCAGCTGTCAACAATGTTTG+TGG 0.523589 4.4:-18350730 MS.gene34999:CDS
CCTGTTCTCAAGCAGAATAC+CGG 0.523975 4.4:-18352545 MS.gene34999:CDS
GCTAATCCCATAAATAAAGT+AGG 0.526869 4.4:+18351280 None:intergenic
TGACAGGATTATAGTTCCCC+TGG 0.528093 4.4:-18350604 MS.gene34999:CDS
CAGTCTGCAGGCTTCAATGC+GGG 0.529912 4.4:+18351574 None:intergenic
TCAAGCAGAATACCGGCCAT+TGG 0.534006 4.4:-18352538 MS.gene34999:CDS
CTGCAAGAAATGTGACCACT+TGG 0.536880 4.4:-18352304 MS.gene34999:CDS
GGATTCAAGAAAATGCAGGC+TGG 0.539579 4.4:-18351321 MS.gene34999:CDS
CTGATTGCATTTGGGACTGG+TGG 0.541290 4.4:-18351487 MS.gene34999:CDS
AGAGAATGATTCATCACTTG+TGG 0.547544 4.4:-18352903 MS.gene34999:CDS
GGATTATAGTTCCCCTGGCA+AGG 0.552068 4.4:-18350599 MS.gene34999:CDS
GCTGAACCGAAACAGTCTGC+AGG 0.553406 4.4:+18351562 None:intergenic
TGGACAAGCTTTCCAATGGC+CGG 0.554291 4.4:+18352526 None:intergenic
TTCTAGCTGATTCTTACTTG+GGG 0.554518 4.4:-18352244 MS.gene34999:CDS
GAGACTTTGCACTGTGACAC+AGG 0.556132 4.4:-18350826 MS.gene34999:CDS
CAGGTAGAGACCAAAGAAGA+AGG 0.556686 4.4:+18351507 None:intergenic
CTACACAAGGTGGAAATCCT+GGG 0.557038 4.4:-18350221 MS.gene34999:CDS
AGAAGGCGATACACCGGAGA+TGG 0.558838 4.4:-18352590 MS.gene34999:CDS
AATAAAAGTGCCTGATACAA+GGG 0.559865 4.4:+18351348 None:intergenic
GGTCACATTTCTTGCAGCAG+AGG 0.560390 4.4:+18352310 None:intergenic
TTGCACTGTGACACAGGTGG+AGG 0.563549 4.4:-18350820 MS.gene34999:CDS
TGATACTGATCCCCAAGAAA+GGG 0.568693 4.4:-18351422 MS.gene34999:CDS
TATTGTGAAGGAGTGGCTGA+AGG 0.569410 4.4:+18351535 None:intergenic
ACCTCAGTATTTCTTATTTG+GGG 0.570703 4.4:-18350397 MS.gene34999:CDS
CCCTTCTTAACCCTTTCTTG+GGG 0.573006 4.4:+18351412 None:intergenic
TCAACAATGTTTGTGGAACA+AGG 0.575130 4.4:-18350723 MS.gene34999:CDS
AGCAGAAGTATTCACAAACG+TGG 0.576227 4.4:-18350373 MS.gene34999:CDS
CTCACCCGCAAACTACATCA+AGG 0.577887 4.4:-18352338 MS.gene34999:CDS
ACTATGATGGACACAAGTAT+TGG 0.580948 4.4:-18350699 MS.gene34999:CDS
GCAGAAGTATTCACAAACGT+GGG 0.583778 4.4:-18350372 MS.gene34999:CDS
GTTCTGCAATTCTAGGAAGT+CGG 0.585591 4.4:-18351099 MS.gene34999:CDS
CCCCAAGAAAGGGTTAAGAA+GGG 0.586458 4.4:-18351412 MS.gene34999:CDS
TATTACATCAAAACTTGAGA+GGG 0.586875 4.4:+18350653 None:intergenic
AGAAATGTGACCACTTGGCA+AGG 0.587065 4.4:-18352299 MS.gene34999:CDS
GAGCAGAGAAGGCGATACAC+CGG 0.588196 4.4:-18352596 MS.gene34999:CDS
TCACAGTGCAAAGTCTCCAC+AGG 0.589021 4.4:+18350832 None:intergenic
CCTGTAAATTTCCTTGCCAG+GGG 0.589579 4.4:+18350588 None:intergenic
TAGAGCATAGTGATGAGCTA+AGG 0.592451 4.4:-18351072 MS.gene34999:intron
ATTGTGAAGGAGTGGCTGAA+GGG 0.595876 4.4:+18351536 None:intergenic
GTTCGGTTGACTTTGAAGGG+AGG 0.598065 4.4:-18352568 MS.gene34999:CDS
TTATTACATCAAAACTTGAG+AGG 0.610706 4.4:+18350652 None:intergenic
GGCAGCATATTGTGAAGGAG+TGG 0.612387 4.4:+18351528 None:intergenic
AAGAAGGCAGCATATTGTGA+AGG 0.613318 4.4:+18351523 None:intergenic
GCGGTATTTGGTTGTCCCTG+AGG 0.614533 4.4:-18351155 MS.gene34999:CDS
GTGTGGATTCAAGAAAATGC+AGG 0.620641 4.4:-18351325 MS.gene34999:CDS
GCTAGAATAGCTCCAATGAG+TGG 0.625100 4.4:+18352260 None:intergenic
AGCACTGCATAAACTCCGCA+TGG 0.625778 4.4:+18350314 None:intergenic
GTGGCGACTATTCTAACCTG+TGG 0.627719 4.4:-18350848 MS.gene34999:CDS
TGTGGAACAAGGAACTATGA+TGG 0.627968 4.4:-18350712 MS.gene34999:CDS
CCCAAGAAAGGGTTAAGAAG+GGG 0.629712 4.4:-18351411 MS.gene34999:CDS
ATTCAAGAAAATGCAGGCTG+GGG 0.634416 4.4:-18351319 MS.gene34999:CDS
AGGTTATCTGGAATCCACCC+AGG 0.636000 4.4:+18350204 None:intergenic
TCTGATGCTGAGAGGAAAAG+TGG 0.637296 4.4:-18350867 MS.gene34999:CDS
TAAATGGACAAGCTTTCCAA+TGG 0.641099 4.4:+18352522 None:intergenic
ACAAGAGACTACTATCCTCA+GGG 0.646212 4.4:+18351140 None:intergenic
ATAGCAAGTGCCTTGCCAAG+TGG 0.646727 4.4:+18352289 None:intergenic
ACCAATCTCCAGACGCCATG+CGG 0.649819 4.4:-18350329 MS.gene34999:CDS
CTATAGTGTCTGATGCTGAG+AGG 0.651673 4.4:-18350875 MS.gene34999:CDS
AAATTTACAGGCAAAGAACG+AGG 0.651776 4.4:-18350576 MS.gene34999:CDS
GCCCCAAAAGACGACACACA+TGG 0.665180 4.4:+18351457 None:intergenic
GCCCCAAATAAGAAATACTG+AGG 0.668426 4.4:+18350396 None:intergenic
GTAACTCACTTCACTACACA+AGG 0.671941 4.4:-18350234 MS.gene34999:CDS
AAGTGATGAATCATTCTCTG+TGG 0.673151 4.4:+18352907 None:intergenic
ACTCACTTCACTACACAAGG+TGG 0.673462 4.4:-18350231 MS.gene34999:CDS
ACTTTGCACTGTGACACAGG+TGG 0.676355 4.4:-18350823 MS.gene34999:CDS
CAGAAGTATTCACAAACGTG+GGG 0.695368 4.4:-18350371 MS.gene34999:CDS
ATTCCAGATAACCTGAACAA+AGG 0.696262 4.4:-18350195 MS.gene34999:CDS
CACAAGGTGGAAATCCTGGG+TGG 0.700761 4.4:-18350218 MS.gene34999:CDS
AAACTTGAGAGGGAAGCTGG+TGG 0.700875 4.4:+18350663 None:intergenic
ACAGTCTGCAGGCTTCAATG+CGG 0.704360 4.4:+18351573 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATATATTTTTTTTTAAAT+GGG + chr4.4:18351237-18351256 None:intergenic 0.0%
!! AATAATTACAATGAAATAAA+TGG + chr4.4:18350816-18350835 None:intergenic 10.0%
!! ATTTGAAAAAATATACTAAT+TGG + chr4.4:18352589-18352608 None:intergenic 10.0%
!! GTATAATAAATATAATTAAG+TGG + chr4.4:18350337-18350356 None:intergenic 10.0%
!!! AAGTTTTTAACATTAAATTA+AGG - chr4.4:18351128-18351147 MS.gene34999:CDS 10.0%
!! AAATATATAAGGTCAATATT+AGG - chr4.4:18351247-18351266 MS.gene34999:CDS 15.0%
!! ATGAATTAAACTAATAATCA+TGG + chr4.4:18350359-18350378 None:intergenic 15.0%
!! CTTCAAAAAAAATTGAATTA+AGG - chr4.4:18351155-18351174 MS.gene34999:CDS 15.0%
!! TAATGTTAAAAACTTCAATA+TGG + chr4.4:18351123-18351142 None:intergenic 15.0%
!!! TAATTTGTCTGAATAATAAT+TGG - chr4.4:18351971-18351990 MS.gene34999:intron 15.0%
!! AGACAATATTTGAAGAAAAA+AGG + chr4.4:18350166-18350185 None:intergenic 20.0%
!! GATCATAGTAAATTCAAAAT+AGG + chr4.4:18351067-18351086 None:intergenic 20.0%
!! TAATAGACAATATCACAAAA+TGG + chr4.4:18352046-18352065 None:intergenic 20.0%
!! TGCTAAATATTGAGATAAAA+AGG + chr4.4:18352486-18352505 None:intergenic 20.0%
!!! ATGTAATAAGTGTTATTTTC+TGG - chr4.4:18352368-18352387 MS.gene34999:CDS 20.0%
!!! ATGTCTTATTGTTTTAATGA+AGG - chr4.4:18352092-18352111 MS.gene34999:intron 20.0%
!!! GTAAATTCAAAATAGGTTTT+CGG + chr4.4:18351060-18351079 None:intergenic 20.0%
!!! TGTAATAAGTGTTATTTTCT+GGG - chr4.4:18352369-18352388 MS.gene34999:CDS 20.0%
!!! TTTTACTTTTCTATCAACAT+AGG - chr4.4:18351633-18351652 MS.gene34999:intron 20.0%
!!! TTTTTTCTTCTTGTACTTTT+TGG + chr4.4:18352908-18352927 None:intergenic 20.0%
! AAAAGAATTTGTACTCCAAA+TGG + chr4.4:18350968-18350987 None:intergenic 25.0%
! AACAGCATTAAAAACTATTC+CGG + chr4.4:18352251-18352270 None:intergenic 25.0%
! AACTGATTAGAGAATAATGA+AGG - chr4.4:18351002-18351021 MS.gene34999:intron 25.0%
! AATGAAAAAAAAGCTTACCT+AGG + chr4.4:18350511-18350530 None:intergenic 25.0%
! ACTTAGCTTCTTAAATATGT+TGG - chr4.4:18352867-18352886 MS.gene34999:intron 25.0%
! ATAATTAAGTGGATATGACA+AGG + chr4.4:18350326-18350345 None:intergenic 25.0%
! ATACCTCAGTATTTCTTATT+TGG - chr4.4:18352607-18352626 MS.gene34999:intron 25.0%
! GTAATTATTACAATCCGATA+AGG - chr4.4:18350827-18350846 MS.gene34999:CDS 25.0%
! TATTACATCAAAACTTGAGA+GGG + chr4.4:18352356-18352375 None:intergenic 25.0%
! TGCAATTTATTGTATCCATT+TGG - chr4.4:18350950-18350969 MS.gene34999:intron 25.0%
! TTATTACATCAAAACTTGAG+AGG + chr4.4:18352357-18352376 None:intergenic 25.0%
! TTGTGATATTGTCTATTAAG+AGG - chr4.4:18352048-18352067 MS.gene34999:intron 25.0%
!! ATTTTGTATCTGAATTCTGA+AGG - chr4.4:18350385-18350404 MS.gene34999:CDS 25.0%
!! TACCTCAGTATTTCTTATTT+GGG - chr4.4:18352608-18352627 MS.gene34999:intron 25.0%
!!! AAATTAAATCTTCTGAAGCA+AGG + chr4.4:18352019-18352038 None:intergenic 25.0%
!!! GTCATCTTGATTATTTTTTC+TGG - chr4.4:18352833-18352852 MS.gene34999:intron 25.0%
!!! TTATTTATGGGATTAGCTAT+AGG - chr4.4:18351732-18351751 MS.gene34999:intron 25.0%
AAAAGCTTACCTAGGATAAA+TGG + chr4.4:18350503-18350522 None:intergenic 30.0%
AACAAAGCATATGTAAGCAA+AGG + chr4.4:18351325-18351344 None:intergenic 30.0%
AAGAAAAAAGGTAGAGAGAA+AGG + chr4.4:18350154-18350173 None:intergenic 30.0%
ACTTGGAACATTCTTGTAAT+AGG + chr4.4:18351810-18351829 None:intergenic 30.0%
ACTTGTGTTAATCTGTAACA+AGG - chr4.4:18351363-18351382 MS.gene34999:CDS 30.0%
AGAAAAAAGGTAGAGAGAAA+GGG + chr4.4:18350153-18350172 None:intergenic 30.0%
GATCCGAATATTTCCAATTT+GGG - chr4.4:18352222-18352241 MS.gene34999:CDS 30.0%
GCTAATCCCATAAATAAAGT+AGG + chr4.4:18351729-18351748 None:intergenic 30.0%
TCTACAGATTTCAAAAACCA+GGG - chr4.4:18351778-18351797 MS.gene34999:intron 30.0%
TGATATGAAATACGAATCAC+AGG - chr4.4:18350587-18350606 MS.gene34999:CDS 30.0%
TGATCCGAATATTTCCAATT+TGG - chr4.4:18352221-18352240 MS.gene34999:CDS 30.0%
TTACAGCAAAAGCAATACTT+TGG + chr4.4:18350278-18350297 None:intergenic 30.0%
TTAGAGAATAATGAAGGATG+TGG - chr4.4:18351008-18351027 MS.gene34999:intron 30.0%
! ACCTCAGTATTTCTTATTTG+GGG - chr4.4:18352609-18352628 MS.gene34999:intron 30.0%
! ATTCTAGCTGATTCTTACTT+GGG - chr4.4:18350761-18350780 MS.gene34999:CDS 30.0%
! GACAAGGTATTTACAATTAC+TGG + chr4.4:18350310-18350329 None:intergenic 30.0%
! GGCATTCCTACTTTATTTAT+GGG - chr4.4:18351720-18351739 MS.gene34999:intron 30.0%
! TATTCTAGCTGATTCTTACT+TGG - chr4.4:18350760-18350779 MS.gene34999:CDS 30.0%
! TTTTCAGAGTTGCAAAGAAT+GGG - chr4.4:18352454-18352473 MS.gene34999:intron 30.0%
!! AATAAAAGTGCCTGATACAA+GGG + chr4.4:18351661-18351680 None:intergenic 30.0%
!!! TATTTTTTCTGGCTTTTAGC+TGG - chr4.4:18352844-18352863 MS.gene34999:intron 30.0%
!!! TGGTTTTTGAAATCTGTAGA+GGG + chr4.4:18351778-18351797 None:intergenic 30.0%
AAAGCTTGTCCATTTATCCT+AGG - chr4.4:18350491-18350510 MS.gene34999:CDS 35.0%
AAATTTACAGGCAAAGAACG+AGG - chr4.4:18352430-18352449 MS.gene34999:intron 35.0%
AAGTGATGAATCATTCTCTG+TGG + chr4.4:18350102-18350121 None:intergenic 35.0%
AATCAAGATGACCTTTGTTC+AGG + chr4.4:18352825-18352844 None:intergenic 35.0%
ACTATAATCCTGTCATAGAC+TGG + chr4.4:18352397-18352416 None:intergenic 35.0%
AGAGAATGATTCATCACTTG+TGG - chr4.4:18350103-18350122 MS.gene34999:CDS 35.0%
AGGTCAATATTAGGAACAGT+TGG - chr4.4:18351256-18351275 MS.gene34999:CDS 35.0%
ATACTAATTGGTACAGCAAC+AGG + chr4.4:18352577-18352596 None:intergenic 35.0%
ATGCAAAAATTAGACGTGTG+TGG + chr4.4:18350928-18350947 None:intergenic 35.0%
ATTCCAGATAACCTGAACAA+AGG - chr4.4:18352811-18352830 MS.gene34999:intron 35.0%
CAGCAACAGGTTTATCAATA+AGG + chr4.4:18352564-18352583 None:intergenic 35.0%
CAGCCCAAATTGGAAATATT+CGG + chr4.4:18352228-18352247 None:intergenic 35.0%
CATTTCCTTGATGTAGTTTG+CGG + chr4.4:18350676-18350695 None:intergenic 35.0%
CTACAGATTTCAAAAACCAG+GGG - chr4.4:18351779-18351798 MS.gene34999:intron 35.0%
CTCTACAGATTTCAAAAACC+AGG - chr4.4:18351777-18351796 MS.gene34999:intron 35.0%
TAAATGGACAAGCTTTCCAA+TGG + chr4.4:18350487-18350506 None:intergenic 35.0%
TACAAGAATGTTCCAAGTTG+TGG - chr4.4:18351812-18351831 MS.gene34999:intron 35.0%
TACAGATTTCAAAAACCAGG+GGG - chr4.4:18351780-18351799 MS.gene34999:intron 35.0%
TCAACAATGTTTGTGGAACA+AGG - chr4.4:18352283-18352302 MS.gene34999:CDS 35.0%
TGACAAACTAGACTGTAACA+TGG - chr4.4:18350226-18350245 MS.gene34999:CDS 35.0%
! ATCTTTTCAGAAGCGGTATT+TGG - chr4.4:18351839-18351858 MS.gene34999:intron 35.0%
! ATTTCCTTGATGTAGTTTGC+GGG + chr4.4:18350675-18350694 None:intergenic 35.0%
! CGGCATTCCTACTTTATTTA+TGG - chr4.4:18351719-18351738 MS.gene34999:intron 35.0%
! CTATGAGAATAGAGCCTTAT+CGG + chr4.4:18350844-18350863 None:intergenic 35.0%
! CTTGCTATAATGCTGGAAAA+TGG - chr4.4:18350892-18350911 MS.gene34999:CDS 35.0%
! CTTTTCAGAGTTGCAAAGAA+TGG - chr4.4:18352453-18352472 MS.gene34999:intron 35.0%
! GCTATAGGAAGCTTCTTTTT+AGG - chr4.4:18351747-18351766 MS.gene34999:intron 35.0%
! TATCAGGCACTTTTATTGTG+TGG - chr4.4:18351664-18351683 MS.gene34999:intron 35.0%
! TCTCAATCTTGCTATAATGC+TGG - chr4.4:18350885-18350904 MS.gene34999:CDS 35.0%
! TGCAATGGAAGGAAAGTTTT+TGG + chr4.4:18351089-18351108 None:intergenic 35.0%
! TTCTAGCTGATTCTTACTTG+GGG - chr4.4:18350762-18350781 MS.gene34999:CDS 35.0%
!! ACTATGATGGACACAAGTAT+TGG - chr4.4:18352307-18352326 MS.gene34999:CDS 35.0%
!! AGAAAAGCACTGGAAAATTG+AGG - chr4.4:18351032-18351051 MS.gene34999:intron 35.0%
!! CAATAAAAGTGCCTGATACA+AGG + chr4.4:18351662-18351681 None:intergenic 35.0%
!! CTTTGGACACTGATTTACAA+TGG + chr4.4:18350261-18350280 None:intergenic 35.0%
!! TAAATCTTCTGAAGCAAGGA+AGG + chr4.4:18352015-18352034 None:intergenic 35.0%
!! TACATTGACTCTTTCTGCAT+CGG - chr4.4:18351407-18351426 MS.gene34999:CDS 35.0%
!!! AGAAGGGGTCATTTTTCAAT+TGG - chr4.4:18351610-18351629 MS.gene34999:CDS 35.0%
!!! CTGGTTTTTGAAATCTGTAG+AGG + chr4.4:18351779-18351798 None:intergenic 35.0%
!!! GGTTTTTGAAATCTGTAGAG+GGG + chr4.4:18351777-18351796 None:intergenic 35.0%
AAGAAGGCAGCATATTGTGA+AGG + chr4.4:18351486-18351505 None:intergenic 40.0%
ACAAGAGACTACTATCCTCA+GGG + chr4.4:18351869-18351888 None:intergenic 40.0%
AGAATTGCAGAACTCTTGTC+TGG + chr4.4:18351900-18351919 None:intergenic 40.0%
AGTAGAATCGATGCAATGGA+AGG + chr4.4:18351100-18351119 None:intergenic 40.0%
AGTTATGTTCAGTGCTCAAG+TGG + chr4.4:18351197-18351216 None:intergenic 40.0%
ATATTTCCAATTTGGGCTGC+CGG - chr4.4:18352229-18352248 MS.gene34999:CDS 40.0%
ATGATACTGATCCCCAAGAA+AGG - chr4.4:18351583-18351602 MS.gene34999:CDS 40.0%
ATGGAGTAGAATCGATGCAA+TGG + chr4.4:18351104-18351123 None:intergenic 40.0%
ATTCAAGAAAATGCAGGCTG+GGG - chr4.4:18351687-18351706 MS.gene34999:intron 40.0%
CAATATGCTGCCTTCTTCTT+TGG - chr4.4:18351489-18351508 MS.gene34999:CDS 40.0%
CAATGAATGTTGCGAACGTT+TGG - chr4.4:18350610-18350629 MS.gene34999:CDS 40.0%
CACAAACATTGTTGACAGCT+GGG + chr4.4:18352278-18352297 None:intergenic 40.0%
GAAAAGATGCTACCACAACT+TGG + chr4.4:18351827-18351846 None:intergenic 40.0%
GACAAGAGTTCTGCAATTCT+AGG - chr4.4:18351900-18351919 MS.gene34999:intron 40.0%
GAGTTGCAAAGAATGGGAAT+TGG - chr4.4:18352460-18352479 MS.gene34999:intron 40.0%
GATTCAAGAAAATGCAGGCT+GGG - chr4.4:18351686-18351705 MS.gene34999:intron 40.0%
GCCCCAAATAAGAAATACTG+AGG + chr4.4:18352613-18352632 None:intergenic 40.0%
GGAAGAAGAATCTCTTCTTC+AGG - chr4.4:18350124-18350143 MS.gene34999:CDS 40.0%
GTAACTCACTTCACTACACA+AGG - chr4.4:18352772-18352791 MS.gene34999:intron 40.0%
GTCTCTACCTGATTGCATTT+GGG - chr4.4:18351511-18351530 MS.gene34999:CDS 40.0%
GTGTGGATTCAAGAAAATGC+AGG - chr4.4:18351681-18351700 MS.gene34999:intron 40.0%
TACAAGAGACTACTATCCTC+AGG + chr4.4:18351870-18351889 None:intergenic 40.0%
TAGAGCATAGTGATGAGCTA+AGG - chr4.4:18351934-18351953 MS.gene34999:intron 40.0%
TAGCAAGATTGAGATGTGTG+TGG + chr4.4:18350879-18350898 None:intergenic 40.0%
TGATACTGATCCCCAAGAAA+GGG - chr4.4:18351584-18351603 MS.gene34999:CDS 40.0%
TGCTATCTTACACCACTCAT+TGG - chr4.4:18350734-18350753 MS.gene34999:CDS 40.0%
TGTGGAACAAGGAACTATGA+TGG - chr4.4:18352294-18352313 MS.gene34999:CDS 40.0%
! AAACCATGTGTGTCGTCTTT+TGG - chr4.4:18351546-18351565 MS.gene34999:CDS 40.0%
! AACCATGTGTGTCGTCTTTT+GGG - chr4.4:18351547-18351566 MS.gene34999:CDS 40.0%
! GTTCTGCAATTCTAGGAAGT+CGG - chr4.4:18351907-18351926 MS.gene34999:intron 40.0%
! TAACAAGGCTGTTTCATGCA+GGG - chr4.4:18351378-18351397 MS.gene34999:CDS 40.0%
! TGACCTTTGTTCAGGTTATC+TGG + chr4.4:18352817-18352836 None:intergenic 40.0%
! TGGTAGCATCTTTTCAGAAG+CGG - chr4.4:18351832-18351851 MS.gene34999:intron 40.0%
!! AGCAGAAGTATTCACAAACG+TGG - chr4.4:18352633-18352652 MS.gene34999:intron 40.0%
!! ATGGTTCGGTTGACTTTGAA+GGG - chr4.4:18350435-18350454 MS.gene34999:CDS 40.0%
!! CAGAAGTATTCACAAACGTG+GGG - chr4.4:18352635-18352654 MS.gene34999:intron 40.0%
!! GCACTTTTGACTACTTCACT+GGG - chr4.4:18352718-18352737 MS.gene34999:intron 40.0%
!! GCAGAAGTATTCACAAACGT+GGG - chr4.4:18352634-18352653 MS.gene34999:intron 40.0%
AAAGTCAACCGAACCATCTC+CGG + chr4.4:18350432-18350451 None:intergenic 45.0%
AACATAGGAGCCCTTGTATC+AGG - chr4.4:18351648-18351667 MS.gene34999:intron 45.0%
ACCCCTTCTTAACCCTTTCT+TGG + chr4.4:18351599-18351618 None:intergenic 45.0%
ACTACACAAGGTGGAAATCC+TGG - chr4.4:18352784-18352803 MS.gene34999:intron 45.0%
ACTCACTTCACTACACAAGG+TGG - chr4.4:18352775-18352794 MS.gene34999:intron 45.0%
AGAAATGTGACCACTTGGCA+AGG - chr4.4:18350707-18350726 MS.gene34999:CDS 45.0%
ATTCTTACTTGGGGCGATAC+TGG - chr4.4:18350771-18350790 MS.gene34999:CDS 45.0%
ATTGTGAAGGAGTGGCTGAA+GGG + chr4.4:18351473-18351492 None:intergenic 45.0%
CAGGTAGAGACCAAAGAAGA+AGG + chr4.4:18351502-18351521 None:intergenic 45.0%
CCACAAACATTGTTGACAGC+TGG + chr4.4:18352279-18352298 None:intergenic 45.0%
CCAGCTGTCAACAATGTTTG+TGG - chr4.4:18352276-18352295 MS.gene34999:CDS 45.0%
CCCAAGAAAGGGTTAAGAAG+GGG - chr4.4:18351595-18351614 MS.gene34999:CDS 45.0%
CCCCAAGAAAGGGTTAAGAA+GGG - chr4.4:18351594-18351613 MS.gene34999:CDS 45.0%
CCCCTTCTTAACCCTTTCTT+GGG + chr4.4:18351598-18351617 None:intergenic 45.0%
CCCTTCTTAACCCTTTCTTG+GGG + chr4.4:18351597-18351616 None:intergenic 45.0%
CCTGTAAATTTCCTTGCCAG+GGG + chr4.4:18352421-18352440 None:intergenic 45.0%
CCTGTTCTCAAGCAGAATAC+CGG - chr4.4:18350461-18350480 MS.gene34999:CDS 45.0%
CTACACAAGGTGGAAATCCT+GGG - chr4.4:18352785-18352804 MS.gene34999:intron 45.0%
CTATAGTGTCTGATGCTGAG+AGG - chr4.4:18352131-18352150 MS.gene34999:intron 45.0%
CTGCAAGAAATGTGACCACT+TGG - chr4.4:18350702-18350721 MS.gene34999:CDS 45.0%
GCCTGTAAATTTCCTTGCCA+GGG + chr4.4:18352422-18352441 None:intergenic 45.0%
GCTAGAATAGCTCCAATGAG+TGG + chr4.4:18350749-18350768 None:intergenic 45.0%
GGATTCAAGAAAATGCAGGC+TGG - chr4.4:18351685-18351704 MS.gene34999:intron 45.0%
GGTCTCTACCTGATTGCATT+TGG - chr4.4:18351510-18351529 MS.gene34999:CDS 45.0%
TATTGTGAAGGAGTGGCTGA+AGG + chr4.4:18351474-18351493 None:intergenic 45.0%
TCAAAACTTGAGAGGGAAGC+TGG + chr4.4:18352349-18352368 None:intergenic 45.0%
TCCCCAAGAAAGGGTTAAGA+AGG - chr4.4:18351593-18351612 MS.gene34999:CDS 45.0%
TCTGATGCTGAGAGGAAAAG+TGG - chr4.4:18352139-18352158 MS.gene34999:intron 45.0%
TGACAGGATTATAGTTCCCC+TGG - chr4.4:18352402-18352421 MS.gene34999:intron 45.0%
TGCCTGTAAATTTCCTTGCC+AGG + chr4.4:18352423-18352442 None:intergenic 45.0%
TGCGAACGTTTGGCATACTA+TGG - chr4.4:18350620-18350639 MS.gene34999:CDS 45.0%
TTGAAGCCTGCAGACTGTTT+CGG - chr4.4:18351438-18351457 MS.gene34999:CDS 45.0%
! ACCATGTGTGTCGTCTTTTG+GGG - chr4.4:18351548-18351567 MS.gene34999:CDS 45.0%
! AGGATGTGGAAGAAAAGCAC+TGG - chr4.4:18351022-18351041 MS.gene34999:intron 45.0%
! ATGTAGTTTGCGGGTGAGAT+AGG + chr4.4:18350666-18350685 None:intergenic 45.0%
! GTAACAAGGCTGTTTCATGC+AGG - chr4.4:18351377-18351396 MS.gene34999:CDS 45.0%
! TACCTGATTGCATTTGGGAC+TGG - chr4.4:18351516-18351535 MS.gene34999:CDS 45.0%
! TTCTGAAGGATGAGCAGAGA+AGG - chr4.4:18350399-18350418 MS.gene34999:CDS 45.0%
!! GATGGTTCGGTTGACTTTGA+AGG - chr4.4:18350434-18350453 MS.gene34999:CDS 45.0%
!! GGCACTTTTGACTACTTCAC+TGG - chr4.4:18352717-18352736 MS.gene34999:intron 45.0%
!! CATTTAAAAAAAAATATATA+AGG - chr4.4:18351236-18351255 MS.gene34999:CDS 5.0%
!!! CTTATATATTTTTTTTTAAA+TGG + chr4.4:18351238-18351257 None:intergenic 5.0%
AAACTTGAGAGGGAAGCTGG+TGG + chr4.4:18352346-18352365 None:intergenic 50.0%
ACAGTCTGCAGGCTTCAATG+CGG + chr4.4:18351436-18351455 None:intergenic 50.0%
ACTATTCCGGCAGCCCAAAT+TGG + chr4.4:18352238-18352257 None:intergenic 50.0%
ACTTTGCACTGTGACACAGG+TGG - chr4.4:18352183-18352202 MS.gene34999:intron 50.0%
AGCACTGCATAAACTCCGCA+TGG + chr4.4:18352695-18352714 None:intergenic 50.0%
ATAGCAAGTGCCTTGCCAAG+TGG + chr4.4:18350720-18350739 None:intergenic 50.0%
CACCAGTCCCAAATGCAATC+AGG + chr4.4:18351521-18351540 None:intergenic 50.0%
CCCCTGGCAAGGAAATTTAC+AGG - chr4.4:18352418-18352437 MS.gene34999:intron 50.0%
CCGGTATTCTGCTTGAGAAC+AGG + chr4.4:18350464-18350483 None:intergenic 50.0%
CTCACCCGCAAACTACATCA+AGG - chr4.4:18350668-18350687 MS.gene34999:CDS 50.0%
CTTGTAATAGGACTTCCCCC+TGG + chr4.4:18351798-18351817 None:intergenic 50.0%
GAAAATGCAGGCTGGGGTAT+TGG - chr4.4:18351693-18351712 MS.gene34999:intron 50.0%
GAGACTTTGCACTGTGACAC+AGG - chr4.4:18352180-18352199 MS.gene34999:intron 50.0%
GCGGAGTTTATGCAGTGCTT+TGG - chr4.4:18352696-18352715 MS.gene34999:intron 50.0%
GGATTATAGTTCCCCTGGCA+AGG - chr4.4:18352407-18352426 MS.gene34999:intron 50.0%
GGCAGCATATTGTGAAGGAG+TGG + chr4.4:18351481-18351500 None:intergenic 50.0%
GGTCACATTTCTTGCAGCAG+AGG + chr4.4:18350699-18350718 None:intergenic 50.0%
GTGGCGACTATTCTAACCTG+TGG - chr4.4:18352158-18352177 MS.gene34999:intron 50.0%
TCAAGCAGAATACCGGCCAT+TGG - chr4.4:18350468-18350487 MS.gene34999:CDS 50.0%
TCACAGTGCAAAGTCTCCAC+AGG + chr4.4:18352177-18352196 None:intergenic 50.0%
TCTGGGTTCCAGTCTATGAC+AGG - chr4.4:18352386-18352405 MS.gene34999:CDS 50.0%
TGGACAAGCTTTCCAATGGC+CGG + chr4.4:18350483-18350502 None:intergenic 50.0%
! AGGTTATCTGGAATCCACCC+AGG + chr4.4:18352805-18352824 None:intergenic 50.0%
! CTGATTGCATTTGGGACTGG+TGG - chr4.4:18351519-18351538 MS.gene34999:CDS 50.0%
! GTTCGGTTGACTTTGAAGGG+AGG - chr4.4:18350438-18350457 MS.gene34999:CDS 50.0%
ACCAATCTCCAGACGCCATG+CGG - chr4.4:18352677-18352696 MS.gene34999:intron 55.0%
AGAAGGCGATACACCGGAGA+TGG - chr4.4:18350416-18350435 MS.gene34999:CDS 55.0%
CACAAGGTGGAAATCCTGGG+TGG - chr4.4:18352788-18352807 MS.gene34999:intron 55.0%
CAGTCTGCAGGCTTCAATGC+GGG + chr4.4:18351435-18351454 None:intergenic 55.0%
CATAAACTCCGCATGGCGTC+TGG + chr4.4:18352688-18352707 None:intergenic 55.0%
GAGCAGAGAAGGCGATACAC+CGG - chr4.4:18350410-18350429 MS.gene34999:CDS 55.0%
GCCCCAAAAGACGACACACA+TGG + chr4.4:18351552-18351571 None:intergenic 55.0%
GCGATACACCGGAGATGGTT+CGG - chr4.4:18350421-18350440 MS.gene34999:CDS 55.0%
GCGGTATTTGGTTGTCCCTG+AGG - chr4.4:18351851-18351870 MS.gene34999:intron 55.0%
GCTGAACCGAAACAGTCTGC+AGG + chr4.4:18351447-18351466 None:intergenic 55.0%
TTGCACTGTGACACAGGTGG+AGG - chr4.4:18352186-18352205 MS.gene34999:intron 55.0%
!! GCAGGCTGGGGTATTGGATT+CGG - chr4.4:18351699-18351718 MS.gene34999:intron 55.0%
TCCGCATGGCGTCTGGAGAT+TGG + chr4.4:18352681-18352700 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 18350092 18352936 18350092 ID=MS.gene34999
chr4.4 mRNA 18350092 18352936 18350092 ID=MS.gene34999.t1;Parent=MS.gene34999
chr4.4 exon 18352883 18352936 18352883 ID=MS.gene34999.t1.exon1;Parent=MS.gene34999.t1
chr4.4 CDS 18352883 18352936 18352883 ID=cds.MS.gene34999.t1;Parent=MS.gene34999.t1
chr4.4 exon 18352516 18352621 18352516 ID=MS.gene34999.t1.exon2;Parent=MS.gene34999.t1
chr4.4 CDS 18352516 18352621 18352516 ID=cds.MS.gene34999.t1;Parent=MS.gene34999.t1
chr4.4 exon 18352202 18352419 18352202 ID=MS.gene34999.t1.exon3;Parent=MS.gene34999.t1
chr4.4 CDS 18352202 18352419 18352202 ID=cds.MS.gene34999.t1;Parent=MS.gene34999.t1
chr4.4 exon 18351073 18351629 18351073 ID=MS.gene34999.t1.exon4;Parent=MS.gene34999.t1
chr4.4 CDS 18351073 18351629 18351073 ID=cds.MS.gene34999.t1;Parent=MS.gene34999.t1
chr4.4 exon 18350092 18350914 18350092 ID=MS.gene34999.t1.exon5;Parent=MS.gene34999.t1
chr4.4 CDS 18350092 18350914 18350092 ID=cds.MS.gene34999.t1;Parent=MS.gene34999.t1
Gene Sequence

>MS.gene34999

ATGGGTTCCACAGAGAATGATTCATCACTTGTGGAAGAAGAATCTCTTCTTCAGGTTTCCCTTTCTCTCTACCTTTTTTCTTCAAATATTGTCTTGAAATGTTGAAAGACACTAGACTGTGATAATAGTAATATTGACAAACTAGACTGTAACATGGTGACTGAATCCATTGTAAATCAGTGTCCAAAGTATTGCTTTTGCTGTAAAATGTTTTTCCAGTAATTGTAAATACCTTGTCATATCCACTTAATTATATTTATTATACCATGATTATTAGTTTAATTCATTGTGTTATTTTGTATCTGAATTCTGAAGGATGAGCAGAGAAGGCGATACACCGGAGATGGTTCGGTTGACTTTGAAGGGAGGCCTGTTCTCAAGCAGAATACCGGCCATTGGAAAGCTTGTCCATTTATCCTAGGTAAGCTTTTTTTTCATTTCTCTTATGTATCAATTAATGTGTGTGTGTGTGTGTTTGTGCGTAACATTTTTGTTTGATATGAAATACGAATCACAGGCAATGAATGTTGCGAACGTTTGGCATACTATGGCATTGCGAAAAATCTTGTCACCTATCTCACCCGCAAACTACATCAAGGAAATGCCTCTGCTGCAAGAAATGTGACCACTTGGCAAGGCACTTGCTATCTTACACCACTCATTGGAGCTATTCTAGCTGATTCTTACTTGGGGCGATACTGGACTATTGCTGTTTTCTCTGCCATTTATTTCATTGTAATTATTACAATCCGATAAGGCTCTATTCTCATAGTTAAATGATTCTCCACACACATCTCAATCTTGCTATAATGCTGGAAAATGGAAATGATTCTCCACACACGTCTAATTTTTGCATGATGCAATTTATTGTATCCATTTGGAGTACAAATTCTTTTTTACATGTCATTGTAACTGATTAGAGAATAATGAAGGATGTGGAAGAAAAGCACTGGAAAATTGAGGATCCGAAAACCTATTTTGAATTTACTATGATCCAAAAACTTTCCTTCCATTGCATCGATTCTACTCCATATTGAAGTTTTTAACATTAAATTAAGGTATTCTTCAAAAAAAATTGAATTAAGGTATTTTTTGATTGATTCCACTTGAGCACTGAACATAACTCAGAATGAATGCACTTTCCCATTTAAAAAAAAATATATAAGGTCAATATTAGGAACAGTTGGAATTTGTCTCAGTATGTTTATATCATCGTATTTTTCTATGCATCCTTTGCTTACATATGCTTTGTTGTTTAATTATCTTTACTTACTTGTGTTAATCTGTAACAAGGCTGTTTCATGCAGGGAATGTGTACATTGACTCTTTCTGCATCGGTTCCCGCATTGAAGCCTGCAGACTGTTTCGGTTCAGCATGCCCTTCAGCCACTCCTTCACAATATGCTGCCTTCTTCTTTGGTCTCTACCTGATTGCATTTGGGACTGGTGGTATTAAACCATGTGTGTCGTCTTTTGGGGCAGATCAGTTTGATGATACTGATCCCCAAGAAAGGGTTAAGAAGGGGTCATTTTTCAATTGGTTTTACTTTTCTATCAACATAGGAGCCCTTGTATCAGGCACTTTTATTGTGTGGATTCAAGAAAATGCAGGCTGGGGTATTGGATTCGGCATTCCTACTTTATTTATGGGATTAGCTATAGGAAGCTTCTTTTTAGGCACACCCCTCTACAGATTTCAAAAACCAGGGGGAAGTCCTATTACAAGAATGTTCCAAGTTGTGGTAGCATCTTTTCAGAAGCGGTATTTGGTTGTCCCTGAGGATAGTAGTCTCTTGTACGAGACACCAGACAAGAGTTCTGCAATTCTAGGAAGTCGGAAACTAGAGCATAGTGATGAGCTAAGGTAATTGTTTACTATTAATTTGTCTGAATAATAATTGGTTCAGTTATTGATGACGTCCTTCCTTGCTTCAGAAGATTTAATTTAACACCATTTTGTGATATTGTCTATTAAGAGGAATTTTGCTTTACGTTTTTCAATGTCTTATTGTTTTAATGAAGGTGTCTCGATAAAGCAGCTATAGTGTCTGATGCTGAGAGGAAAAGTGGCGACTATTCTAACCTGTGGAGACTTTGCACTGTGACACAGGTGGAGGAATTGAAAATTTTGATCCGAATATTTCCAATTTGGGCTGCCGGAATAGTTTTTAATGCTGTTTATGCCCAGCTGTCAACAATGTTTGTGGAACAAGGAACTATGATGGACACAAGTATTGGTTCATTCAAAATACCACCAGCTTCCCTCTCAAGTTTTGATGTAATAAGTGTTATTTTCTGGGTTCCAGTCTATGACAGGATTATAGTTCCCCTGGCAAGGAAATTTACAGGCAAAGAACGAGGCTTTTCAGAGTTGCAAAGAATGGGAATTGGCCTTTTTATCTCAATATTTAGCATGTTAGCAGCTGTTGTTGTTGAGATTAAGCGTCTGCAGCTTGCAAAAGAGCTTGACCTTATTGATAAACCTGTTGCTGTACCAATTAGTATATTTTTTCAAATACCTCAGTATTTCTTATTTGGGGCAGCAGAAGTATTCACAAACGTGGGGCAACTTGAATTCTTTTATGACCAATCTCCAGACGCCATGCGGAGTTTATGCAGTGCTTTGGCACTTTTGACTACTTCACTGGGAAATTACTTGAGTTCTTTCATTCTCACTGTTGTAACTCACTTCACTACACAAGGTGGAAATCCTGGGTGGATTCCAGATAACCTGAACAAAGGTCATCTTGATTATTTTTTCTGGCTTTTAGCTGGACTTAGCTTCTTAAATATGTTGGTGTACATAGTTTCAGCCAAAAAGTACAAGAAGAAAAAAGCTTCTTAG
Protein sequence

>MS.gene34999.t1

MGSTENDSSLVEEESLLQDEQRRRYTGDGSVDFEGRPVLKQNTGHWKACPFILGNECCERLAYYGIAKNLVTYLTRKLHQGNASAARNVTTWQGTCYLTPLIGAILADSYLGRYWTIAVFSAIYFIGMCTLTLSASVPALKPADCFGSACPSATPSQYAAFFFGLYLIAFGTGGIKPCVSSFGADQFDDTDPQERVKKGSFFNWFYFSINIGALVSGTFIVWIQENAGWGIGFGIPTLFMGLAIGSFFLGTPLYRFQKPGGSPITRMFQVVVASFQKRYLVVPEDSSLLYETPDKSSAILGSRKLEHSDELRCLDKAAIVSDAERKSGDYSNLWRLCTVTQVEELKILIRIFPIWAAGIVFNAVYAQLSTMFVEQGTMMDTSIGSFKIPPASLSSFDVISVIFWVPVYDRIIVPLARKFTGKERGFSELQRMGIGLFISIFSMLAAVVVEIKRLQLAKELDLIDKPVAVPISIFFQIPQYFLFGAAEVFTNVGQLEFFYDQSPDAMRSLCSALALLTTSLGNYLSSFILTVVTHFTTQGGNPGWIPDNLNKGHLDYFFWLLAGLSFLNMLVYIVSAKKYKKKKAS