AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene033170

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033170.t1 MTR_4g107580 93.698 603 36 2 1 603 1 601 0.0 1163
MS.gene033170.t1 MTR_4g107510 68.761 573 175 2 3 575 16 584 0.0 846
MS.gene033170.t1 MTR_4g107530 65.789 608 187 4 9 603 5 604 0.0 822
MS.gene033170.t1 MTR_5g038060 47.150 579 290 6 28 597 34 605 0.0 552
MS.gene033170.t1 MTR_8g469480 45.739 575 293 8 27 594 40 602 0.0 526
MS.gene033170.t1 MTR_4g107610 41.404 570 319 6 12 578 18 575 3.99e-155 459
MS.gene033170.t1 MTR_5g038190 38.908 568 334 6 12 575 10 568 1.70e-148 441
MS.gene033170.t1 MTR_8g469470 38.087 575 337 8 11 575 16 581 1.98e-146 436
MS.gene033170.t1 MTR_7g064780 38.957 575 336 7 1 570 1 565 2.29e-140 421
MS.gene033170.t1 MTR_8g103233 39.500 600 338 12 5 600 2 580 8.12e-139 416
MS.gene033170.t1 MTR_5g055000 36.960 579 337 8 5 575 2 560 1.02e-133 403
MS.gene033170.t1 MTR_7g064800 37.479 595 351 9 1 583 1 586 6.02e-133 401
MS.gene033170.t1 MTR_7g088825 38.609 575 331 11 28 595 25 584 9.45e-132 399
MS.gene033170.t1 MTR_4g015080 37.608 577 343 8 1 575 10 571 4.08e-130 394
MS.gene033170.t1 MTR_3g462710 37.591 548 329 5 29 575 28 563 1.72e-128 389
MS.gene033170.t1 MTR_7g088790 36.425 593 359 10 7 593 10 590 3.99e-128 390
MS.gene033170.t1 MTR_7g067010 37.843 547 327 6 29 575 28 561 1.42e-127 387
MS.gene033170.t1 MTR_2g101640 37.153 576 337 8 28 601 26 578 4.11e-126 385
MS.gene033170.t1 MTR_4g011280 34.512 594 374 8 6 597 14 594 3.22e-124 379
MS.gene033170.t1 MTR_1g009200 38.645 546 316 9 28 568 30 561 3.81e-124 379
MS.gene033170.t1 MTR_2g101650 37.887 549 325 9 28 572 27 563 2.26e-123 377
MS.gene033170.t1 MTR_7g088820 34.609 601 374 10 1 596 3 589 3.22e-122 374
MS.gene033170.t1 MTR_3g112460 34.642 586 355 10 10 594 11 569 2.52e-118 363
MS.gene033170.t1 MTR_3g112450 34.812 586 361 5 7 592 5 569 4.77e-117 360
MS.gene033170.t1 MTR_3g112470 35.450 567 343 8 28 594 26 569 6.15e-117 359
MS.gene033170.t1 MTR_2g101560 34.983 606 372 9 7 601 9 603 1.77e-115 358
MS.gene033170.t1 MTR_2g101610 33.879 611 373 9 7 602 9 603 5.93e-114 354
MS.gene033170.t1 MTR_2g101590 33.826 609 375 10 7 602 9 602 2.61e-112 350
MS.gene033170.t1 MTR_7g088820 35.261 536 330 9 66 596 4 527 1.04e-111 345
MS.gene033170.t1 MTR_7g098200 33.670 594 367 10 15 601 19 592 1.43e-110 344
MS.gene033170.t1 MTR_5g014150 32.937 589 369 10 1 573 8 586 1.62e-110 344
MS.gene033170.t1 MTR_5g012290 31.864 590 382 8 12 596 14 588 4.18e-110 342
MS.gene033170.t1 MTR_3g462710 39.246 451 262 6 126 575 15 454 2.62e-109 336
MS.gene033170.t1 MTR_1g026740 33.157 567 363 10 8 572 1 553 5.64e-109 339
MS.gene033170.t1 MTR_5g012270 34.116 554 346 8 28 575 32 572 8.80e-109 339
MS.gene033170.t1 MTR_1g026770 33.750 560 357 8 28 586 18 564 6.13e-108 336
MS.gene033170.t1 MTR_7g098200 33.277 592 362 9 15 601 19 582 1.04e-107 336
MS.gene033170.t1 MTR_1g026750 33.962 583 361 11 13 588 2 567 2.84e-107 334
MS.gene033170.t1 MTR_4g098800 33.453 559 352 5 29 579 40 586 8.83e-107 334
MS.gene033170.t1 MTR_7g005930 31.282 585 385 6 8 592 18 585 3.68e-106 332
MS.gene033170.t1 MTR_4g098800 33.453 559 352 5 29 579 105 651 8.49e-106 333
MS.gene033170.t1 MTR_4g019580 35.701 549 334 9 28 575 39 569 1.78e-105 330
MS.gene033170.t1 MTR_1g057460 33.795 577 341 11 1 572 24 564 2.45e-105 330
MS.gene033170.t1 MTR_3g069420 34.578 616 365 14 1 600 1 594 7.17e-105 329
MS.gene033170.t1 MTR_3g014790 34.083 578 350 8 8 580 1 552 3.93e-104 326
MS.gene033170.t1 MTR_4g101380 35.739 568 350 9 28 591 30 586 1.37e-103 325
MS.gene033170.t1 MTR_3g072300 35.159 566 346 10 29 590 28 576 1.48e-103 325
MS.gene033170.t1 MTR_7g098160 33.218 578 362 10 29 601 37 595 3.69e-103 325
MS.gene033170.t1 MTR_3g069500 33.002 603 365 12 2 593 4 578 1.18e-102 323
MS.gene033170.t1 MTR_3g069440 34.501 571 345 12 29 589 37 588 1.43e-102 323
MS.gene033170.t1 MTR_3g005010 34.955 555 310 9 28 575 33 543 2.43e-101 318
MS.gene033170.t1 MTR_7g088820 35.642 491 299 9 111 596 1 479 2.77e-101 316
MS.gene033170.t1 MTR_7g065080 33.846 585 343 12 8 575 1 558 5.35e-101 318
MS.gene033170.t1 MTR_7g010820 33.216 566 370 6 29 592 30 589 1.37e-100 318
MS.gene033170.t1 MTR_7g098040 31.818 572 370 8 29 597 38 592 8.49e-100 317
MS.gene033170.t1 MTR_7g013270 31.979 566 367 6 28 591 93 642 4.29e-99 315
MS.gene033170.t1 MTR_0294s0020 34.159 565 322 11 24 574 20 548 1.91e-97 308
MS.gene033170.t1 MTR_7g098230 31.779 579 363 9 34 603 35 590 2.46e-97 309
MS.gene033170.t1 MTR_1g116930 31.711 596 373 10 3 595 8 572 3.50e-97 309
MS.gene033170.t1 MTR_1g057460 33.524 525 312 9 53 572 1 493 4.76e-97 306
MS.gene033170.t1 MTR_5g085850 33.800 571 342 8 28 592 33 573 8.86e-97 308
MS.gene033170.t1 MTR_2g093640 34.429 578 340 11 5 575 2 547 1.81e-96 306
MS.gene033170.t1 MTR_1g042310 32.409 577 368 9 28 601 29 586 4.28e-96 306
MS.gene033170.t1 MTR_8g038880 32.280 601 348 10 8 586 1 564 3.18e-95 303
MS.gene033170.t1 MTR_7g098090 31.787 582 370 10 29 601 37 600 4.83e-95 304
MS.gene033170.t1 MTR_2g093160 33.679 579 343 13 5 575 2 547 6.96e-95 301
MS.gene033170.t1 MTR_1g075440 31.524 571 374 7 15 582 18 574 9.68e-95 302
MS.gene033170.t1 MTR_4g033325 33.803 568 320 11 24 574 20 548 3.51e-94 300
MS.gene033170.t1 MTR_7g098220 31.509 603 388 9 4 601 9 591 3.81e-94 301
MS.gene033170.t1 MTR_4g114340 34.358 553 338 11 28 575 27 559 4.12e-94 300
MS.gene033170.t1 MTR_1g042310 32.389 565 360 9 40 601 4 549 1.87e-93 298
MS.gene033170.t1 MTR_4g033315 33.929 560 324 11 29 581 35 555 3.37e-92 295
MS.gene033170.t1 MTR_4g033310 33.929 560 324 11 29 581 35 555 3.37e-92 295
MS.gene033170.t1 MTR_0236s0050 33.397 527 310 10 53 572 1 493 5.36e-92 293
MS.gene033170.t1 MTR_7g006540 31.779 579 367 8 11 575 48 612 8.45e-92 296
MS.gene033170.t1 MTR_7g098200 34.337 498 300 10 111 601 1 478 1.40e-91 291
MS.gene033170.t1 MTR_7g065080 34.274 496 295 9 84 575 3 471 6.03e-91 290
MS.gene033170.t1 MTR_7g098180 30.623 578 377 8 29 601 35 593 7.13e-91 293
MS.gene033170.t1 MTR_1g042310 32.316 557 355 9 48 601 5 542 7.54e-91 291
MS.gene033170.t1 MTR_2g093180 32.927 574 355 10 5 575 2 548 2.62e-90 290
MS.gene033170.t1 MTR_1g085090 32.021 559 349 11 28 582 22 553 3.95e-90 291
MS.gene033170.t1 MTR_4g033330 33.955 536 310 10 29 557 35 533 4.60e-89 286
MS.gene033170.t1 MTR_7g098220 31.802 566 361 9 41 601 2 547 1.05e-88 285
MS.gene033170.t1 MTR_1g041835 33.031 551 352 7 29 577 31 566 1.27e-88 286
MS.gene033170.t1 MTR_1g041875 32.638 527 330 8 28 551 32 536 5.87e-88 285
MS.gene033170.t1 MTR_7g111260 31.552 580 361 13 11 582 2 553 3.10e-87 283
MS.gene033170.t1 MTR_2g005130 30.466 558 347 9 25 575 40 563 1.76e-86 281
MS.gene033170.t1 MTR_3g100980 32.000 575 354 15 28 592 19 566 8.13e-86 278
MS.gene033170.t1 MTR_1g041815 32.035 565 367 7 29 591 31 580 1.95e-85 278
MS.gene033170.t1 MTR_2g017750 30.313 607 395 11 3 595 10 602 2.30e-85 278
MS.gene033170.t1 MTR_4g033375 33.137 510 292 11 24 518 21 496 5.64e-83 269
MS.gene033170.t1 MTR_4g123537 30.390 589 376 12 1 583 17 577 2.58e-82 270
MS.gene033170.t1 MTR_1g041885 31.774 513 325 8 48 557 5 495 5.43e-81 265
MS.gene033170.t1 MTR_4g033395 32.877 511 292 11 24 518 21 496 5.62e-81 263
MS.gene033170.t1 MTR_3g100980 30.680 603 353 17 28 592 19 594 8.13e-80 263
MS.gene033170.t1 MTR_4g107670 30.034 586 361 11 17 579 16 575 1.58e-79 262
MS.gene033170.t1 MTR_4g033325 34.086 443 257 8 139 574 10 424 1.23e-76 250
MS.gene033170.t1 MTR_4g033325 33.264 478 273 10 104 574 12 450 1.45e-76 251
MS.gene033170.t1 MTR_5g038380 29.352 586 364 10 17 579 20 578 1.72e-76 254
MS.gene033170.t1 MTR_8g469310 31.095 566 379 8 17 579 18 575 2.46e-76 254
MS.gene033170.t1 MTR_4g033385 32.727 440 266 8 142 575 37 452 1.72e-74 245
MS.gene033170.t1 MTR_8g087780 29.322 590 376 10 5 575 7 574 1.97e-74 249
MS.gene033170.t1 MTR_8g087810 28.645 583 390 11 5 575 7 575 1.80e-70 238
MS.gene033170.t1 MTR_7g098150 32.319 427 270 6 29 450 37 449 1.60e-68 230
MS.gene033170.t1 MTR_6g008690 30.090 555 333 13 42 579 24 540 4.24e-65 223
MS.gene033170.t1 MTR_1g009170 33.738 412 208 12 150 556 7 358 1.09e-63 214
MS.gene033170.t1 MTR_4g024930 26.389 576 375 11 4 573 21 553 3.27e-56 199
MS.gene033170.t1 MTR_7g098210 27.172 541 288 12 48 581 5 446 5.24e-54 190
MS.gene033170.t1 MTR_4g033310 36.111 288 170 5 294 581 218 491 8.32e-50 180
MS.gene033170.t1 MTR_4g033310 35.758 165 95 2 29 193 35 188 5.23e-21 97.1
MS.gene033170.t1 MTR_7g051880 26.154 520 337 9 53 572 53 525 2.19e-42 160
MS.gene033170.t1 MTR_7g052600 26.346 520 336 10 53 572 53 525 7.83e-42 159
MS.gene033170.t1 MTR_7g051830 25.577 520 340 9 53 572 53 525 3.60e-39 151
MS.gene033170.t1 MTR_3g065350 35.227 176 103 2 339 514 1 165 3.53e-24 99.8
MS.gene033170.t1 MTR_8g091265 38.655 119 69 4 298 414 3 119 3.94e-19 84.7
MS.gene033170.t1 MTR_7g064160 34.545 110 71 1 1 109 4 113 4.02e-15 72.4
MS.gene033170.t1 MTR_2g093650 41.304 92 51 2 356 447 43 131 4.14e-12 66.2
MS.gene033170.t1 MTR_4g033390 31.183 93 62 1 490 582 30 120 6.52e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene033170.t1 AT5G62680 60.245 571 220 4 11 579 31 596 0.0 726
MS.gene033170.t1 AT1G18880 57.601 592 239 6 7 593 2 586 0.0 714
MS.gene033170.t1 AT3G47960 59.788 567 222 4 14 579 51 612 0.0 699
MS.gene033170.t1 AT1G69870 46.633 594 289 9 12 596 44 618 0.0 541
MS.gene033170.t1 AT1G27080 41.156 588 324 9 16 598 4 574 1.46e-152 451
MS.gene033170.t1 AT5G28470 39.162 549 315 5 28 572 20 553 3.54e-144 429
MS.gene033170.t1 AT5G28470 39.162 549 313 6 28 572 20 551 1.74e-141 422
MS.gene033170.t1 AT1G68570 40.385 572 329 8 9 575 4 568 3.13e-141 423
MS.gene033170.t1 AT1G27080 40.619 549 305 8 54 598 1 532 8.12e-139 414
MS.gene033170.t1 AT1G68570 40.665 541 312 7 37 575 5 538 2.20e-136 409
MS.gene033170.t1 AT1G69860 39.455 550 310 5 28 575 21 549 2.28e-133 401
MS.gene033170.t1 AT1G52190 35.130 575 354 10 8 572 1 566 1.16e-129 394
MS.gene033170.t1 AT3G54140 36.746 547 335 8 29 575 28 563 2.93e-127 386
MS.gene033170.t1 AT3G54140 36.746 547 335 8 29 575 28 563 2.93e-127 386
MS.gene033170.t1 AT5G01180 36.063 574 354 8 3 575 2 563 3.67e-125 380
MS.gene033170.t1 AT5G01180 36.063 574 354 8 3 575 2 563 3.67e-125 380
MS.gene033170.t1 AT5G01180 36.063 574 354 8 3 575 2 563 3.67e-125 380
MS.gene033170.t1 AT3G16180 34.635 589 368 9 9 591 7 584 2.46e-124 379
MS.gene033170.t1 AT2G02040 36.380 547 338 6 29 575 45 581 1.22e-121 372
MS.gene033170.t1 AT1G62200 37.273 550 327 6 29 576 56 589 5.34e-121 370
MS.gene033170.t1 AT1G62200 35.813 578 326 8 29 576 70 632 9.31e-116 358
MS.gene033170.t1 AT1G62200 35.813 578 326 8 29 576 56 618 1.01e-115 358
MS.gene033170.t1 AT3G45650 35.284 564 331 9 28 588 25 557 1.75e-111 345
MS.gene033170.t1 AT3G54450 34.188 585 337 10 11 583 2 550 3.87e-111 344
MS.gene033170.t1 AT3G45660 33.972 574 346 8 12 583 8 550 4.08e-108 336
MS.gene033170.t1 AT3G45660 33.972 574 346 8 12 583 8 550 4.08e-108 336
MS.gene033170.t1 AT3G45660 33.972 574 346 8 12 583 29 571 8.97e-108 336
MS.gene033170.t1 AT3G45650 33.729 590 331 10 28 588 25 583 1.66e-105 330
MS.gene033170.t1 AT2G40460 34.957 575 345 9 29 598 28 578 3.62e-105 329
MS.gene033170.t1 AT4G21680 33.574 554 355 5 25 574 28 572 3.64e-105 330
MS.gene033170.t1 AT3G45680 34.099 566 334 10 28 586 23 556 5.68e-104 325
MS.gene033170.t1 AT2G02020 34.552 547 307 6 29 574 46 542 4.65e-102 320
MS.gene033170.t1 AT3G53960 33.788 586 360 9 2 575 4 573 1.16e-101 321
MS.gene033170.t1 AT3G45720 32.982 570 347 8 17 583 11 548 1.57e-101 319
MS.gene033170.t1 AT2G37900 34.608 549 335 9 28 575 41 566 1.92e-101 320
MS.gene033170.t1 AT1G32450 30.794 617 399 10 4 603 9 614 5.06e-99 314
MS.gene033170.t1 AT2G26690 33.391 578 365 9 11 586 10 569 2.09e-96 306
MS.gene033170.t1 AT3G21670 33.813 556 335 8 28 577 32 560 1.17e-95 305
MS.gene033170.t1 AT3G45710 31.369 577 366 9 9 583 5 553 7.29e-95 302
MS.gene033170.t1 AT5G13400 31.552 580 381 7 15 587 53 623 1.20e-94 303
MS.gene033170.t1 AT3G45700 32.522 575 348 10 8 579 1 538 3.01e-94 300
MS.gene033170.t1 AT2G26690 34.239 552 343 9 37 586 3 536 3.41e-94 300
MS.gene033170.t1 AT1G59740 31.993 572 367 10 5 571 19 573 5.84e-94 300
MS.gene033170.t1 AT2G37900 34.455 505 307 9 72 575 6 487 7.09e-93 295
MS.gene033170.t1 AT1G12110 31.525 590 373 9 12 591 14 582 1.92e-92 296
MS.gene033170.t1 AT5G19640 30.944 572 367 9 28 597 62 607 2.63e-91 294
MS.gene033170.t1 AT2G26690 34.133 542 337 9 47 586 1 524 2.98e-91 291
MS.gene033170.t1 AT1G22540 33.392 569 337 10 17 578 22 555 1.47e-90 290
MS.gene033170.t1 AT5G46050 32.804 567 365 7 27 591 28 580 2.10e-90 291
MS.gene033170.t1 AT1G72125 32.594 586 353 10 8 578 1 559 3.66e-90 290
MS.gene033170.t1 AT1G22550 32.470 579 358 8 8 579 11 563 3.82e-89 287
MS.gene033170.t1 AT5G46040 31.514 568 363 8 29 591 31 577 2.10e-88 286
MS.gene033170.t1 AT3G45690 31.359 574 320 12 12 583 6 507 4.60e-88 283
MS.gene033170.t1 AT1G22570 33.573 557 338 9 28 578 33 563 3.32e-86 279
MS.gene033170.t1 AT1G72130 31.777 557 326 8 28 578 28 536 1.82e-85 276
MS.gene033170.t1 AT1G33440 30.333 600 357 13 1 572 1 567 6.90e-84 274
MS.gene033170.t1 AT1G22570 33.151 549 335 9 36 578 4 526 1.13e-82 269
MS.gene033170.t1 AT1G27040 30.986 568 356 7 17 574 19 560 1.21e-82 270
MS.gene033170.t1 AT1G27040 30.986 568 356 7 17 574 15 556 1.93e-82 270
MS.gene033170.t1 AT1G72120 30.887 586 359 8 8 578 1 555 5.30e-82 268
MS.gene033170.t1 AT2G02020 32.299 548 297 11 29 574 46 521 2.24e-81 265
MS.gene033170.t1 AT1G72140 29.432 581 347 11 16 575 14 552 1.17e-80 265
MS.gene033170.t1 AT1G27040 31.502 546 338 7 39 574 9 528 1.29e-80 264
MS.gene033170.t1 AT1G69850 30.351 570 379 7 17 575 17 579 1.49e-79 262
MS.gene033170.t1 AT5G46050 32.988 482 308 6 111 591 1 468 6.69e-75 247
MS.gene033170.t1 AT1G72130 31.828 443 258 6 142 578 14 418 1.04e-71 237
MS.gene033170.t1 AT1G72130 31.828 443 258 6 142 578 14 418 1.04e-71 237
MS.gene033170.t1 AT5G62730 28.673 565 342 11 34 578 6 529 2.26e-67 229
MS.gene033170.t1 AT5G62730 28.673 565 342 11 34 578 65 588 5.07e-67 229
MS.gene033170.t1 AT5G14940 29.234 561 342 15 42 582 24 549 1.29e-65 224
MS.gene033170.t1 AT3G25260 27.458 539 328 13 39 572 35 515 4.04e-60 209
MS.gene033170.t1 AT3G01350 27.368 570 357 13 42 588 24 559 3.82e-59 207
MS.gene033170.t1 AT3G25280 25.806 527 332 15 52 572 48 521 1.14e-48 177
MS.gene033170.t1 AT5G11570 34.231 260 158 2 313 572 230 476 2.59e-46 170
MS.gene033170.t1 AT5G11570 34.091 220 137 4 27 243 15 229 1.65e-35 140
MS.gene033170.t1 AT2G38100 23.273 550 355 19 49 583 14 511 4.58e-26 112
MS.gene033170.t1 AT2G38100 23.273 550 355 19 49 583 20 517 5.14e-26 112
MS.gene033170.t1 AT2G38100 23.077 546 353 19 53 583 2 495 6.48e-26 112

Find 122 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCACATTTGCTTCCTTTC+TGG 0.050423 8.3:+16148835 MS.gene033170:CDS
CTTCTTTCATTTCCACATTT+TGG 0.089475 8.3:-16149293 None:intergenic
TTTCTATGTGACACTTATTT+TGG 0.139339 8.3:+16148791 MS.gene033170:CDS
GTTACTTTGTATTGTGTTCT+AGG 0.161441 8.3:+16152053 MS.gene033170:CDS
GTTCTACCAATTTGGGTTTC+AGG 0.174285 8.3:+16151380 MS.gene033170:CDS
TAGTAAGAGTTCTACCAATT+TGG 0.200123 8.3:+16151372 MS.gene033170:CDS
TTGTTTGGAAGTTGTTAATT+TGG 0.214202 8.3:+16152030 MS.gene033170:CDS
TCATTTCTACTACTTGGATT+TGG 0.222031 8.3:+16149347 MS.gene033170:CDS
TTTGAAATTTGTGTTGAAAA+AGG 0.232339 8.3:-16151474 None:intergenic
CATTTCTACTACTTGGATTT+GGG 0.250805 8.3:+16149348 MS.gene033170:CDS
TTCTTTCCTGAATCTGTGTT+TGG 0.257557 8.3:-16149437 None:intergenic
ACTTGAACAATATTTGTTAC+AGG 0.278311 8.3:-16149659 None:intergenic
AGTAAGAGTTCTACCAATTT+GGG 0.305859 8.3:+16151373 MS.gene033170:CDS
TTCAATCCAAACACAGATTC+AGG 0.309118 8.3:+16149431 MS.gene033170:CDS
TGTTTGGAAGTTGTTAATTT+GGG 0.324597 8.3:+16152031 MS.gene033170:CDS
CGATGTTAAGCCTAACGATA+TGG 0.336603 8.3:+16151525 MS.gene033170:CDS
TTATTACATGATTACTTGTT+TGG 0.345394 8.3:+16152015 MS.gene033170:CDS
CAAATGTCATTTCTACTACT+TGG 0.348911 8.3:+16149341 MS.gene033170:CDS
AGACCTTGTAACTTAGCATT+TGG 0.352308 8.3:+16149398 MS.gene033170:CDS
GAGAATATGAGAAGTCTTGC+AGG 0.364624 8.3:+16151854 MS.gene033170:CDS
TTTCTCATACACCTCAGTTC+AGG 0.371042 8.3:+16149789 MS.gene033170:CDS
CTTGGTCTTGGAATTCCTGC+TGG 0.371692 8.3:+16149563 MS.gene033170:CDS
GGATTTGAACGAAGGGAGAT+TGG 0.377692 8.3:+16151985 MS.gene033170:CDS
GTGAGTTGGGCTCTTGGTCT+TGG 0.384641 8.3:+16149551 MS.gene033170:CDS
TCTGATGCATTTACTTTGGT+TGG 0.389917 8.3:+16151800 MS.gene033170:CDS
GGATTTGGGCTTATGATAGT+AGG 0.391869 8.3:+16149362 MS.gene033170:CDS
TTTCTCGGTTCAAATCCTAT+AGG 0.394792 8.3:-16151716 None:intergenic
GAACAAAAGAGACACATCTA+TGG 0.397086 8.3:+16148434 MS.gene033170:CDS
TTTGGACGCTACAAAACTCT+TGG 0.399400 8.3:+16148809 MS.gene033170:CDS
TTAAATCTGCAACAGGGAAT+TGG 0.401616 8.3:+16151954 MS.gene033170:CDS
CTTCTACAAAGAATGGGAAT+TGG 0.402115 8.3:+16151617 MS.gene033170:CDS
TGTTGATTACAATTATTCAT+AGG 0.403883 8.3:+16151924 MS.gene033170:CDS
GAATCAATAGTTTCTTCAAT+TGG 0.404560 8.3:+16149462 MS.gene033170:CDS
AGTATGCCTGAAACCCAAAT+TGG 0.407025 8.3:-16151386 None:intergenic
ATGGCTATTAATAATCCTAT+AGG 0.413331 8.3:+16151701 MS.gene033170:CDS
TTGACGGTTAAATCTGCAAC+AGG 0.418463 8.3:+16151947 MS.gene033170:CDS
AATCACACTTCTACAAAGAA+TGG 0.430866 8.3:+16151610 MS.gene033170:CDS
AATGACATTTGTCCTGTACT+TGG 0.433383 8.3:-16149329 None:intergenic
TATACAAGTAAATGTGAGTT+GGG 0.433642 8.3:+16149538 MS.gene033170:CDS
AGCTTGGTGTTGATAGAGTA+AGG 0.434780 8.3:-16149767 None:intergenic
TATTGTGTTCTAGGTGGTAC+AGG 0.435061 8.3:+16152062 MS.gene033170:CDS
GGATCAGATGCAGAACCGTC+AGG 0.440378 8.3:-16151305 None:intergenic
TCATGTGTTATTTATTTCAC+TGG 0.440504 8.3:+16149599 MS.gene033170:CDS
GAAGATATTGACAATATTTG+TGG 0.462744 8.3:-16148732 None:intergenic
TATGTGAAGGTAAAAGCAAC+AGG 0.472904 8.3:+16149632 MS.gene033170:CDS
ATTCCTCAGTTAGCACTAGC+AGG 0.477266 8.3:+16151773 MS.gene033170:CDS
CTTATGATAGTAGGAGCTGC+AGG 0.479508 8.3:+16149371 MS.gene033170:CDS
GTAAATGTGAGTTGGGCTCT+TGG 0.482055 8.3:+16149545 MS.gene033170:CDS
AAGGTCATGCCCTTCATCAT+TGG 0.484294 8.3:+16148519 MS.gene033170:CDS
CAATCTGAAACAAAGATTAG+TGG 0.489778 8.3:+16152148 MS.gene033170:CDS
TTCGGTCCGATTGAAGTGCT+TGG 0.491406 8.3:-16151451 None:intergenic
TACAATTATTCATAGGTTGA+CGG 0.491870 8.3:+16151931 MS.gene033170:CDS
ATCACACTTCTACAAAGAAT+GGG 0.492502 8.3:+16151611 MS.gene033170:CDS
TAGTATCCGGTGTGGTAGAA+TGG 0.495604 8.3:+16151666 MS.gene033170:CDS
AACACAGATTCAGGAAAGAA+AGG 0.495614 8.3:+16149440 MS.gene033170:CDS
ATCTTGCTTACCAATGATGA+AGG 0.496920 8.3:-16148529 None:intergenic
GGCGCGATATCTTCCATGTC+GGG 0.500635 8.3:+16151740 MS.gene033170:CDS
GTTTCAAATATCAACTATAG+AGG 0.503648 8.3:+16148492 MS.gene033170:CDS
AGTCTTCCAAGCACTTCAAT+CGG 0.504676 8.3:+16151445 MS.gene033170:CDS
AGACTAACAATGTGTTCTGT+TGG 0.505478 8.3:-16151427 None:intergenic
AATGATGATGGTACTGATCA+AGG 0.505507 8.3:+16152094 MS.gene033170:CDS
GCAAGACTTCTCATATTCTC+AGG 0.505728 8.3:-16151851 None:intergenic
ATACTATGCATGTTAGTATC+CGG 0.511470 8.3:+16151653 MS.gene033170:CDS
TATATGTGGCTATAAAGAAA+AGG 0.514570 8.3:+16149684 MS.gene033170:CDS
TTTGAGAAGCTAGGAGCCAT+TGG 0.519801 8.3:+16148650 MS.gene033170:CDS
AGATTGGTAACCATATCGTT+AGG 0.522148 8.3:-16151535 None:intergenic
GAAAAGTGACAAAGAAAGTG+TGG 0.527633 8.3:+16148458 MS.gene033170:CDS
ATATACAAGTAAATGTGAGT+TGG 0.529836 8.3:+16149537 MS.gene033170:CDS
TGTTATAATTAATACCAGAA+AGG 0.533754 8.3:-16148849 None:intergenic
ATAGGATTTGAACCGAGAAA+AGG 0.541636 8.3:+16151719 MS.gene033170:CDS
ACGGTTCTGCATCTGATCCA+TGG 0.542827 8.3:+16151309 MS.gene033170:CDS
GCTAGTCTAGCTACCTTGCT+AGG 0.542899 8.3:+16148764 MS.gene033170:CDS
AAGATCGTGTAAGAAGCTGC+AGG 0.544793 8.3:-16151500 None:intergenic
AATATTGTCAATATCTTCAG+TGG 0.545365 8.3:+16148737 MS.gene033170:CDS
CTAGGTGGTACAGGTACAAA+GGG 0.545972 8.3:+16152071 MS.gene033170:CDS
GCTCCAAATGCTAAGTTACA+AGG 0.548040 8.3:-16149401 None:intergenic
AATGAAACATTTGAGAAGCT+AGG 0.548041 8.3:+16148641 MS.gene033170:CDS
GAAACTCAATCTACAAGAAG+AGG 0.560510 8.3:+16149706 MS.gene033170:CDS
CAAATATCAACTATAGAGGA+TGG 0.561443 8.3:+16148496 MS.gene033170:CDS
CTGAGGTGTATGAGAAAGCT+TGG 0.564035 8.3:-16149783 None:intergenic
AACCATTTGTGCAAAAGTGA+AGG 0.567355 8.3:-16149495 None:intergenic
AACAGGGAATTGGTTACCAC+AGG 0.570819 8.3:+16151964 MS.gene033170:CDS
TATAGGATTATTAATAGCCA+TGG 0.573328 8.3:-16151699 None:intergenic
TTATAATAGCTGCTTTGTCA+AGG 0.575374 8.3:-16151262 None:intergenic
GAGGTTTGCTAAGGTACCAA+TGG 0.582527 8.3:-16148666 None:intergenic
TTGTGGCTGCAATATTGCTA+AGG 0.583202 8.3:-16148715 None:intergenic
GTGTGGTAGAATGGAAAAGA+AGG 0.585909 8.3:+16151675 MS.gene033170:CDS
TATTGTTCAAGTGATATATG+TGG 0.591824 8.3:+16149670 MS.gene033170:CDS
TATCAACTATAGAGGATGGA+AGG 0.591866 8.3:+16148500 MS.gene033170:CDS
TCTATGCAGTATACAACAAG+TGG 0.592491 8.3:+16151334 MS.gene033170:CDS
AGGTACAAAGGGAATGATGA+TGG 0.594930 8.3:+16152082 MS.gene033170:CDS
TATCTTCCATGTCGGGAATG+TGG 0.598260 8.3:+16151747 MS.gene033170:CDS
TTGTTATACAACTAACAGCA+TGG 0.599941 8.3:+16149252 MS.gene033170:CDS
GAAAACAGCATCAAACCAGC+AGG 0.602141 8.3:-16149578 None:intergenic
AGCATGCATCCACCAAAATG+TGG 0.602631 8.3:+16149281 MS.gene033170:CDS
CTCCCTTCGTTCAAATCCTG+TGG 0.602701 8.3:-16151980 None:intergenic
ACATGCATAGAACCAAGTAC+AGG 0.603457 8.3:+16149317 MS.gene033170:CDS
TGGAAGTAAATATTATGTGA+AGG 0.605535 8.3:+16149619 MS.gene033170:CDS
TCTAGGTGGTACAGGTACAA+AGG 0.610839 8.3:+16152070 MS.gene033170:CDS
AATCAACCACATTCCCGACA+TGG 0.611889 8.3:-16151753 None:intergenic
TACCACAGGATTTGAACGAA+GGG 0.615700 8.3:+16151978 MS.gene033170:CDS
TGTATACTGCATAGATACCA+TGG 0.620158 8.3:-16151326 None:intergenic
ACTTTGTATTGTGTTCTAGG+TGG 0.620237 8.3:+16152056 MS.gene033170:CDS
TGGTACCTTAGCAAACCTCT+TGG 0.621497 8.3:+16148670 MS.gene033170:CDS
ATAGACCAAGAGGTTTGCTA+AGG 0.624994 8.3:-16148675 None:intergenic
ACATAGAAAAGCTCCTAGCA+AGG 0.626725 8.3:-16148777 None:intergenic
GATCAGATGCAGAACCGTCA+GGG 0.627732 8.3:-16151304 None:intergenic
GATGGTACTGATCAAGGTAG+TGG 0.629248 8.3:+16152100 MS.gene033170:CDS
AGGCGCGATATCTTCCATGT+CGG 0.630902 8.3:+16151739 MS.gene033170:CDS
TTACCACAGGATTTGAACGA+AGG 0.642836 8.3:+16151977 MS.gene033170:CDS
AGAATATGAGAAGTCTTGCA+GGG 0.652741 8.3:+16151855 MS.gene033170:CDS
TCTTGCTTACCAATGATGAA+GGG 0.654105 8.3:-16148528 None:intergenic
TTATGATAGTAGGAGCTGCA+GGG 0.663873 8.3:+16149372 MS.gene033170:CDS
AAAATCACCGGAAAAGAAGG+CGG 0.665576 8.3:+16151587 MS.gene033170:CDS
AGAATGGAAAAGAAGGACCA+TGG 0.673688 8.3:+16151682 MS.gene033170:CDS
AGATCGTGTAAGAAGCTGCA+GGG 0.675641 8.3:-16151499 None:intergenic
CAAGACAAAATAAACCCTGA+CGG 0.679950 8.3:+16151290 MS.gene033170:CDS
CTGATGTAAGATAGACCAAG+AGG 0.685895 8.3:-16148685 None:intergenic
AATCCTGCTAGTGCTAACTG+AGG 0.715827 8.3:-16151776 None:intergenic
ATGCATGTTAGTATCCGGTG+TGG 0.719406 8.3:+16151658 MS.gene033170:CDS
GTGGTTGGTTACCTGAACTG+AGG 0.724471 8.3:-16149800 None:intergenic
TGACGGTTAAATCTGCAACA+GGG 0.748913 8.3:+16151948 MS.gene033170:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTGAATATTGTTTA+AGG + chr8.3:16149062-16149081 MS.gene033170:intron 10.0%
!! ACATAAAAAAAATATAAAAG+AGG + chr8.3:16150145-16150164 MS.gene033170:intron 10.0%
!! CTTATAAAATACAATTTAAT+TGG + chr8.3:16149894-16149913 MS.gene033170:intron 10.0%
!! AAAGAGTAATAAATAGTTAA+AGG - chr8.3:16150350-16150369 None:intergenic 15.0%
!! AATTAGTTGAATATTGTTTA+AGG - chr8.3:16149177-16149196 None:intergenic 15.0%
!! TATAAAAATGTGTTAATAAG+TGG - chr8.3:16151131-16151150 None:intergenic 15.0%
!! TCTATTATCAAATAGAATTT+GGG - chr8.3:16150818-16150837 None:intergenic 15.0%
!! TTCTATTATCAAATAGAATT+TGG - chr8.3:16150819-16150838 None:intergenic 15.0%
!!! ACAAAATTATTTTTCGTTAA+TGG - chr8.3:16150406-16150425 None:intergenic 15.0%
!!! ACTATTTTCAATAAACTATT+TGG + chr8.3:16150790-16150809 MS.gene033170:intron 15.0%
!!! CTATTTTCAATAAACTATTT+GGG + chr8.3:16150791-16150810 MS.gene033170:intron 15.0%
!!! CTATTTTTGTAATCAAATTA+AGG - chr8.3:16150934-16150953 None:intergenic 15.0%
!!! GTAAAAAATTATTTCTGATA+CGG - chr8.3:16149851-16149870 None:intergenic 15.0%
!! AAATGTTCATATGAATTAAC+AGG + chr8.3:16151237-16151256 MS.gene033170:intron 20.0%
!! CTATTATCAAATAGAATTTG+GGG - chr8.3:16150817-16150836 None:intergenic 20.0%
!! CTCATATTATCTTAGAATTT+AGG + chr8.3:16151055-16151074 MS.gene033170:intron 20.0%
!! TATATATCTGTGTGTTTATA+TGG + chr8.3:16150845-16150864 MS.gene033170:intron 20.0%
!! TCTAATTTAACCAACAAAAT+CGG + chr8.3:16151083-16151102 MS.gene033170:intron 20.0%
!! TGATTTCTTCATAAAATGAT+TGG + chr8.3:16151209-16151228 MS.gene033170:intron 20.0%
!! TGTTATAATTAATACCAGAA+AGG - chr8.3:16148852-16148871 None:intergenic 20.0%
!! TTATTACATGATTACTTGTT+TGG + chr8.3:16152015-16152034 MS.gene033170:CDS 20.0%
!! TTATTCAAATAAGATGAACA+TGG - chr8.3:16150770-16150789 None:intergenic 20.0%
!! TTGTAATCAAATTAAGGTTA+AGG - chr8.3:16150928-16150947 None:intergenic 20.0%
!! TTTATGAAGAAATCAACAAA+TGG - chr8.3:16151204-16151223 None:intergenic 20.0%
!!! AATTTAATTGGCTTATTTGT+TGG + chr8.3:16149906-16149925 MS.gene033170:intron 20.0%
!!! ACAAGTTAATTATGATTTGT+TGG - chr8.3:16149149-16149168 None:intergenic 20.0%
!!! TGTTGATTACAATTATTCAT+AGG + chr8.3:16151924-16151943 MS.gene033170:CDS 20.0%
!!! TTATTTTTCGTTAATGGTAT+TGG - chr8.3:16150400-16150419 None:intergenic 20.0%
!!! TTTAACTAGTTAGTTTAGTT+AGG - chr8.3:16149118-16149137 None:intergenic 20.0%
!!! TTTGAAATTTGTGTTGAAAA+AGG - chr8.3:16151477-16151496 None:intergenic 20.0%
! AAAAATGTGTTAATAAGTGG+TGG - chr8.3:16151128-16151147 None:intergenic 25.0%
! AAATATGAGTGAAAGACTAT+AGG + chr8.3:16150030-16150049 MS.gene033170:intron 25.0%
! AATATTGTCAATATCTTCAG+TGG + chr8.3:16148737-16148756 MS.gene033170:CDS 25.0%
! AATTCATTTGCATATTATGC+AGG + chr8.3:16148617-16148636 MS.gene033170:intron 25.0%
! AATTTGTGTTGAAAAAGGTT+CGG - chr8.3:16151472-16151491 None:intergenic 25.0%
! ATATACAAGTAAATGTGAGT+TGG + chr8.3:16149537-16149556 MS.gene033170:CDS 25.0%
! ATGACGTAAAAAGAAAATGA+TGG - chr8.3:16149980-16149999 None:intergenic 25.0%
! ATGGCTATTAATAATCCTAT+AGG + chr8.3:16151701-16151720 MS.gene033170:CDS 25.0%
! GAAGATATTGACAATATTTG+TGG - chr8.3:16148735-16148754 None:intergenic 25.0%
! GAATCAATAGTTTCTTCAAT+TGG + chr8.3:16149462-16149481 MS.gene033170:CDS 25.0%
! GTTTCAAATATCAACTATAG+AGG + chr8.3:16148492-16148511 MS.gene033170:CDS 25.0%
! TAGTTGAATATTGTTTAAGG+TGG - chr8.3:16149174-16149193 None:intergenic 25.0%
! TATACAAGTAAATGTGAGTT+GGG + chr8.3:16149538-16149557 MS.gene033170:CDS 25.0%
! TATAGGATTATTAATAGCCA+TGG - chr8.3:16151702-16151721 None:intergenic 25.0%
! TATATGTGGCTATAAAGAAA+AGG + chr8.3:16149684-16149703 MS.gene033170:CDS 25.0%
! TATTGTTCAAGTGATATATG+TGG + chr8.3:16149670-16149689 MS.gene033170:CDS 25.0%
! TGACGTAAAAAGAAAATGAT+GGG - chr8.3:16149979-16149998 None:intergenic 25.0%
! TGGAAGTAAATATTATGTGA+AGG + chr8.3:16149619-16149638 MS.gene033170:CDS 25.0%
! TGTTTGGAAGTTGTTAATTT+GGG + chr8.3:16152031-16152050 MS.gene033170:CDS 25.0%
! TTGTTTGGAAGTTGTTAATT+TGG + chr8.3:16152030-16152049 MS.gene033170:CDS 25.0%
!! ACTTGAACAATATTTGTTAC+AGG - chr8.3:16149662-16149681 None:intergenic 25.0%
!! ATTTTCTGATGCATTTACTT+TGG + chr8.3:16151796-16151815 MS.gene033170:CDS 25.0%
!! CTCATATTTTCATATGCAAA+CGG - chr8.3:16150019-16150038 None:intergenic 25.0%
!! TACAATTATTCATAGGTTGA+CGG + chr8.3:16151931-16151950 MS.gene033170:CDS 25.0%
!! TTTCTATGTGACACTTATTT+TGG + chr8.3:16148791-16148810 MS.gene033170:CDS 25.0%
!!! AACTCTTTGATTTTGAAACA+GGG + chr8.3:16149224-16149243 MS.gene033170:intron 25.0%
!!! GTTGAAGTATTACTTTTTTG+TGG - chr8.3:16148918-16148937 None:intergenic 25.0%
!!! TAACTCTTTGATTTTGAAAC+AGG + chr8.3:16149223-16149242 MS.gene033170:intron 25.0%
!!! TCATGTGTTATTTATTTCAC+TGG + chr8.3:16149599-16149618 MS.gene033170:CDS 25.0%
AACAATAAGAGTAACATGCA+TGG - chr8.3:16148571-16148590 None:intergenic 30.0%
AATCACACTTCTACAAAGAA+TGG + chr8.3:16151610-16151629 MS.gene033170:CDS 30.0%
AATGAAACATTTGAGAAGCT+AGG + chr8.3:16148641-16148660 MS.gene033170:CDS 30.0%
ACACTTGCACAAATATGTAT+CGG - chr8.3:16150702-16150721 None:intergenic 30.0%
ACGTCAAGAGAACTAAATAA+AGG - chr8.3:16150542-16150561 None:intergenic 30.0%
AGTAAGAGTTCTACCAATTT+GGG + chr8.3:16151373-16151392 MS.gene033170:CDS 30.0%
ATACATATTTGTGCAAGTGT+TGG + chr8.3:16150702-16150721 MS.gene033170:intron 30.0%
ATACTATGCATGTTAGTATC+CGG + chr8.3:16151653-16151672 MS.gene033170:CDS 30.0%
ATCACACTTCTACAAAGAAT+GGG + chr8.3:16151611-16151630 MS.gene033170:CDS 30.0%
CAAATATCAACTATAGAGGA+TGG + chr8.3:16148496-16148515 MS.gene033170:CDS 30.0%
CATTTCTACTACTTGGATTT+GGG + chr8.3:16149348-16149367 MS.gene033170:CDS 30.0%
GTATCTTGTATTTATCCTGT+TGG - chr8.3:16149953-16149972 None:intergenic 30.0%
TAGTAAGAGTTCTACCAATT+TGG + chr8.3:16151372-16151391 MS.gene033170:CDS 30.0%
TCATTTCTACTACTTGGATT+TGG + chr8.3:16149347-16149366 MS.gene033170:CDS 30.0%
TGATACGGAAGTAAAAATAC+TGG - chr8.3:16149836-16149855 None:intergenic 30.0%
TTGTTATACAACTAACAGCA+TGG + chr8.3:16149252-16149271 MS.gene033170:CDS 30.0%
! AAATACTGGACATTGATTAG+TGG - chr8.3:16149822-16149841 None:intergenic 30.0%
! AAGTGCGACAAAAGTAATTT+TGG + chr8.3:16150192-16150211 MS.gene033170:intron 30.0%
! CAAATGTCATTTCTACTACT+TGG + chr8.3:16149341-16149360 MS.gene033170:CDS 30.0%
! CAATCTGAAACAAAGATTAG+TGG + chr8.3:16152148-16152167 MS.gene033170:CDS 30.0%
! CTTCTTTCATTTCCACATTT+TGG - chr8.3:16149296-16149315 None:intergenic 30.0%
! GTTACTTTGTATTGTGTTCT+AGG + chr8.3:16152053-16152072 MS.gene033170:CDS 30.0%
! TTATAATAGCTGCTTTGTCA+AGG - chr8.3:16151265-16151284 None:intergenic 30.0%
!! CATATGCAAACGGATTAAAA+AGG - chr8.3:16150009-16150028 None:intergenic 30.0%
!! TTATTTAGTTCTCTTGACGT+GGG + chr8.3:16150542-16150561 MS.gene033170:intron 30.0%
!! TTTATTTAGTTCTCTTGACG+TGG + chr8.3:16150541-16150560 MS.gene033170:intron 30.0%
!!! ACTCTTTGATTTTGAAACAG+GGG + chr8.3:16149225-16149244 MS.gene033170:intron 30.0%
AAAGCGACTTATAAGAAAGG+AGG - chr8.3:16150253-16150272 None:intergenic 35.0%
AACACAGATTCAGGAAAGAA+AGG + chr8.3:16149440-16149459 MS.gene033170:CDS 35.0%
AACCATTTGTGCAAAAGTGA+AGG - chr8.3:16149498-16149517 None:intergenic 35.0%
AAGCGACTTATAAGAAAGGA+GGG - chr8.3:16150252-16150271 None:intergenic 35.0%
AATGACATTTGTCCTGTACT+TGG - chr8.3:16149332-16149351 None:intergenic 35.0%
AGAAAAATCACCGGAAAAGA+AGG + chr8.3:16151584-16151603 MS.gene033170:CDS 35.0%
AGAATATGAGAAGTCTTGCA+GGG + chr8.3:16151855-16151874 MS.gene033170:CDS 35.0%
AGACAATTGAACAACCTTGA+CGG - chr8.3:16148969-16148988 None:intergenic 35.0%
AGACCTTGTAACTTAGCATT+TGG + chr8.3:16149398-16149417 MS.gene033170:CDS 35.0%
AGACTAACAATGTGTTCTGT+TGG - chr8.3:16151430-16151449 None:intergenic 35.0%
ATAGGATTTGAACCGAGAAA+AGG + chr8.3:16151719-16151738 MS.gene033170:CDS 35.0%
ATCTTGCTTACCAATGATGA+AGG - chr8.3:16148532-16148551 None:intergenic 35.0%
CAAATACGTCTGATCTGATA+TGG - chr8.3:16149009-16149028 None:intergenic 35.0%
CAAGACAAAATAAACCCTGA+CGG + chr8.3:16151290-16151309 MS.gene033170:CDS 35.0%
CCAACAATGAGAAAAATCAC+CGG + chr8.3:16151575-16151594 MS.gene033170:CDS 35.0%
CTTCTACAAAGAATGGGAAT+TGG + chr8.3:16151617-16151636 MS.gene033170:CDS 35.0%
GAAAAGTGACAAAGAAAGTG+TGG + chr8.3:16148458-16148477 MS.gene033170:CDS 35.0%
GAAACTCAATCTACAAGAAG+AGG + chr8.3:16149706-16149725 MS.gene033170:CDS 35.0%
GAACAAAAGAGACACATCTA+TGG + chr8.3:16148434-16148453 MS.gene033170:CDS 35.0%
TAAGAGTAACATGCATGGAA+TGG - chr8.3:16148566-16148585 None:intergenic 35.0%
TATCAACTATAGAGGATGGA+AGG + chr8.3:16148500-16148519 MS.gene033170:CDS 35.0%
TATGTGAAGGTAAAAGCAAC+AGG + chr8.3:16149632-16149651 MS.gene033170:CDS 35.0%
TCCTTTCTTATAAGTCGCTT+TGG + chr8.3:16150252-16150271 MS.gene033170:intron 35.0%
TCTATGCAGTATACAACAAG+TGG + chr8.3:16151334-16151353 MS.gene033170:CDS 35.0%
TCTGATGCATTTACTTTGGT+TGG + chr8.3:16151800-16151819 MS.gene033170:CDS 35.0%
TCTTGCTTACCAATGATGAA+GGG - chr8.3:16148531-16148550 None:intergenic 35.0%
TGTATACTGCATAGATACCA+TGG - chr8.3:16151329-16151348 None:intergenic 35.0%
TTAAATCTGCAACAGGGAAT+TGG + chr8.3:16151954-16151973 MS.gene033170:CDS 35.0%
TTCAATCCAAACACAGATTC+AGG + chr8.3:16149431-16149450 MS.gene033170:CDS 35.0%
TTCTTTCCTGAATCTGTGTT+TGG - chr8.3:16149440-16149459 None:intergenic 35.0%
TTTCTCGGTTCAAATCCTAT+AGG - chr8.3:16151719-16151738 None:intergenic 35.0%
! ACGATTTTTCTGTCGTAGAT+TGG - chr8.3:16151554-16151573 None:intergenic 35.0%
! ACTTTGTATTGTGTTCTAGG+TGG + chr8.3:16152056-16152075 MS.gene033170:CDS 35.0%
! AGATTGGTAACCATATCGTT+AGG - chr8.3:16151538-16151557 None:intergenic 35.0%
! CCTTTCTTATAAGTCGCTTT+GGG + chr8.3:16150253-16150272 MS.gene033170:intron 35.0%
! CTTTCATTTCCACATTTTGG+TGG - chr8.3:16149293-16149312 None:intergenic 35.0%
! TTGCACAAATATGTATCGGT+TGG - chr8.3:16150698-16150717 None:intergenic 35.0%
!! AATGATGATGGTACTGATCA+AGG + chr8.3:16152094-16152113 MS.gene033170:CDS 35.0%
!! ACTATACAAGCCGATTTTGT+TGG - chr8.3:16151096-16151115 None:intergenic 35.0%
!! ATATTTGTGCAAGTGTTGGT+TGG + chr8.3:16150706-16150725 MS.gene033170:intron 35.0%
!! TACCTTCACTTTTGCACAAA+TGG + chr8.3:16149493-16149512 MS.gene033170:CDS 35.0%
!! TCGGTTGGAATTTTAAGTGT+CGG - chr8.3:16150683-16150702 None:intergenic 35.0%
!!! AGGGTTTATTTTGTCTTGTG+GGG - chr8.3:16151288-16151307 None:intergenic 35.0%
!!! CAGGGTTTATTTTGTCTTGT+GGG - chr8.3:16151289-16151308 None:intergenic 35.0%
!!! GAATTTTAAGTGTCGGTAGT+TGG - chr8.3:16150676-16150695 None:intergenic 35.0%
!!! TCAGGGTTTATTTTGTCTTG+TGG - chr8.3:16151290-16151309 None:intergenic 35.0%
AAAATCACCGGAAAAGAAGG+CGG + chr8.3:16151587-16151606 MS.gene033170:CDS 40.0%
AACTCATTCGACGAACCAAT+TGG - chr8.3:16151024-16151043 None:intergenic 40.0%
ACATAGAAAAGCTCCTAGCA+AGG - chr8.3:16148780-16148799 None:intergenic 40.0%
ACATGCATAGAACCAAGTAC+AGG + chr8.3:16149317-16149336 MS.gene033170:CDS 40.0%
AGAATGGAAAAGAAGGACCA+TGG + chr8.3:16151682-16151701 MS.gene033170:CDS 40.0%
AGGTACAAAGGGAATGATGA+TGG + chr8.3:16152082-16152101 MS.gene033170:CDS 40.0%
AGTATGCCTGAAACCCAAAT+TGG - chr8.3:16151389-16151408 None:intergenic 40.0%
AGTCTTCCAAGCACTTCAAT+CGG + chr8.3:16151445-16151464 MS.gene033170:CDS 40.0%
CCCAAAGCGACTTATAAGAA+AGG - chr8.3:16150256-16150275 None:intergenic 40.0%
CGATGTTAAGCCTAACGATA+TGG + chr8.3:16151525-16151544 MS.gene033170:CDS 40.0%
CTGATGTAAGATAGACCAAG+AGG - chr8.3:16148688-16148707 None:intergenic 40.0%
GAGAATATGAGAAGTCTTGC+AGG + chr8.3:16151854-16151873 MS.gene033170:CDS 40.0%
GCAAGACTTCTCATATTCTC+AGG - chr8.3:16151854-16151873 None:intergenic 40.0%
GCTCCAAATGCTAAGTTACA+AGG - chr8.3:16149404-16149423 None:intergenic 40.0%
GGATTTGGGCTTATGATAGT+AGG + chr8.3:16149362-16149381 MS.gene033170:CDS 40.0%
GTGTGGTAGAATGGAAAAGA+AGG + chr8.3:16151675-16151694 MS.gene033170:CDS 40.0%
TACCACAGGATTTGAACGAA+GGG + chr8.3:16151978-16151997 MS.gene033170:CDS 40.0%
TGACGGTTAAATCTGCAACA+GGG + chr8.3:16151948-16151967 MS.gene033170:CDS 40.0%
TTACCACAGGATTTGAACGA+AGG + chr8.3:16151977-16151996 MS.gene033170:CDS 40.0%
TTATGATAGTAGGAGCTGCA+GGG + chr8.3:16149372-16149391 MS.gene033170:CDS 40.0%
TTGACGGTTAAATCTGCAAC+AGG + chr8.3:16151947-16151966 MS.gene033170:CDS 40.0%
TTGTGGCTGCAATATTGCTA+AGG - chr8.3:16148718-16148737 None:intergenic 40.0%
TTTCTCATACACCTCAGTTC+AGG + chr8.3:16149789-16149808 MS.gene033170:CDS 40.0%
TTTGGACGCTACAAAACTCT+TGG + chr8.3:16148809-16148828 MS.gene033170:CDS 40.0%
! ACTGGACATTGATTAGTGGT+TGG - chr8.3:16149818-16149837 None:intergenic 40.0%
! CCGGTGATTTTTCTCATTGT+TGG - chr8.3:16151578-16151597 None:intergenic 40.0%
! GTTCTACCAATTTGGGTTTC+AGG + chr8.3:16151380-16151399 MS.gene033170:CDS 40.0%
! TATTGTGTTCTAGGTGGTAC+AGG + chr8.3:16152062-16152081 MS.gene033170:CDS 40.0%
! TCTTTTCCATTCTACCACAC+CGG - chr8.3:16151675-16151694 None:intergenic 40.0%
!! AGCTTGGTGTTGATAGAGTA+AGG - chr8.3:16149770-16149789 None:intergenic 40.0%
!! ATAGACCAAGAGGTTTGCTA+AGG - chr8.3:16148678-16148697 None:intergenic 40.0%
!! CAAAATCGGCTTGTATAGTG+AGG + chr8.3:16151097-16151116 MS.gene033170:intron 40.0%
AACAGGGAATTGGTTACCAC+AGG + chr8.3:16151964-16151983 MS.gene033170:CDS 45.0%
AAGATCGTGTAAGAAGCTGC+AGG - chr8.3:16151503-16151522 None:intergenic 45.0%
AAGGTCATGCCCTTCATCAT+TGG + chr8.3:16148519-16148538 MS.gene033170:CDS 45.0%
AATCAACCACATTCCCGACA+TGG - chr8.3:16151756-16151775 None:intergenic 45.0%
AATCCTGCTAGTGCTAACTG+AGG - chr8.3:16151779-16151798 None:intergenic 45.0%
ACGACTAAAGCGTGACCAAT+TGG + chr8.3:16151006-16151025 MS.gene033170:intron 45.0%
AGATCGTGTAAGAAGCTGCA+GGG - chr8.3:16151502-16151521 None:intergenic 45.0%
AGCATGCATCCACCAAAATG+TGG + chr8.3:16149281-16149300 MS.gene033170:CDS 45.0%
ATGCATGTTAGTATCCGGTG+TGG + chr8.3:16151658-16151677 MS.gene033170:CDS 45.0%
ATTCCTCAGTTAGCACTAGC+AGG + chr8.3:16151773-16151792 MS.gene033170:CDS 45.0%
CTAGGTGGTACAGGTACAAA+GGG + chr8.3:16152071-16152090 MS.gene033170:CDS 45.0%
CTGAGGTGTATGAGAAAGCT+TGG - chr8.3:16149786-16149805 None:intergenic 45.0%
CTGCACATTTGCTTCCTTTC+TGG + chr8.3:16148835-16148854 MS.gene033170:CDS 45.0%
CTTATGATAGTAGGAGCTGC+AGG + chr8.3:16149371-16149390 MS.gene033170:CDS 45.0%
GAAAACAGCATCAAACCAGC+AGG - chr8.3:16149581-16149600 None:intergenic 45.0%
GGATTTGAACGAAGGGAGAT+TGG + chr8.3:16151985-16152004 MS.gene033170:CDS 45.0%
TAGTATCCGGTGTGGTAGAA+TGG + chr8.3:16151666-16151685 MS.gene033170:CDS 45.0%
TATCTTCCATGTCGGGAATG+TGG + chr8.3:16151747-16151766 MS.gene033170:CDS 45.0%
TCTAGGTGGTACAGGTACAA+AGG + chr8.3:16152070-16152089 MS.gene033170:CDS 45.0%
TGGTACCTTAGCAAACCTCT+TGG + chr8.3:16148670-16148689 MS.gene033170:CDS 45.0%
TTTGAGAAGCTAGGAGCCAT+TGG + chr8.3:16148650-16148669 MS.gene033170:CDS 45.0%
! GAAGATATCGCGCCTTTTCT+CGG - chr8.3:16151734-16151753 None:intergenic 45.0%
! GTAAATGTGAGTTGGGCTCT+TGG + chr8.3:16149545-16149564 MS.gene033170:CDS 45.0%
! TGTGATTCCGCCTTCTTTTC+CGG - chr8.3:16151597-16151616 None:intergenic 45.0%
!! GAGGTTTGCTAAGGTACCAA+TGG - chr8.3:16148669-16148688 None:intergenic 45.0%
!! GATGGTACTGATCAAGGTAG+TGG + chr8.3:16152100-16152119 MS.gene033170:CDS 45.0%
!! GTCACTGTTTTTCTGTGGCT+TGG + chr8.3:16151877-16151896 MS.gene033170:CDS 45.0%
!!! ATAATTTTTTACTATTTAAT+TGG + chr8.3:16149859-16149878 MS.gene033170:intron 5.0%
ACGGTTCTGCATCTGATCCA+TGG + chr8.3:16151309-16151328 MS.gene033170:CDS 50.0%
AGGCGCGATATCTTCCATGT+CGG + chr8.3:16151739-16151758 MS.gene033170:CDS 50.0%
CGTCTGATCTGATATGGCTG+CGG - chr8.3:16149003-16149022 None:intergenic 50.0%
CTCCCTTCGTTCAAATCCTG+TGG - chr8.3:16151983-16152002 None:intergenic 50.0%
GATCAGATGCAGAACCGTCA+GGG - chr8.3:16151307-16151326 None:intergenic 50.0%
GTCTGATCTGATATGGCTGC+GGG - chr8.3:16149002-16149021 None:intergenic 50.0%
TAGAAACTGCGAGCACCAAC+AGG + chr8.3:16149935-16149954 MS.gene033170:intron 50.0%
! GCTAGTCTAGCTACCTTGCT+AGG + chr8.3:16148764-16148783 MS.gene033170:CDS 50.0%
! GTGGTTGGTTACCTGAACTG+AGG - chr8.3:16149803-16149822 None:intergenic 50.0%
! GTTTTACAATCGCGCCGTCA+AGG + chr8.3:16148952-16148971 MS.gene033170:intron 50.0%
! TTCGGTCCGATTGAAGTGCT+TGG - chr8.3:16151454-16151473 None:intergenic 50.0%
!! CTTGGTCTTGGAATTCCTGC+TGG + chr8.3:16149563-16149582 MS.gene033170:CDS 50.0%
!! GCAGGGTCACTGTTTTTCTG+TGG + chr8.3:16151872-16151891 MS.gene033170:CDS 50.0%
GGATCAGATGCAGAACCGTC+AGG - chr8.3:16151308-16151327 None:intergenic 55.0%
GGCGCGATATCTTCCATGTC+GGG + chr8.3:16151740-16151759 MS.gene033170:CDS 55.0%
! GTGAGTTGGGCTCTTGGTCT+TGG + chr8.3:16149551-16149570 MS.gene033170:CDS 55.0%
TGATATGGCTGCGGGTCACG+AGG - chr8.3:16148994-16149013 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 16148432 16152177 16148432 ID=MS.gene033170
chr8.3 mRNA 16148432 16152177 16148432 ID=MS.gene033170.t1;Parent=MS.gene033170
chr8.3 exon 16148432 16148540 16148432 ID=MS.gene033170.t1.exon1;Parent=MS.gene033170.t1
chr8.3 CDS 16148432 16148540 16148432 ID=cds.MS.gene033170.t1;Parent=MS.gene033170.t1
chr8.3 exon 16148639 16148856 16148639 ID=MS.gene033170.t1.exon2;Parent=MS.gene033170.t1
chr8.3 CDS 16148639 16148856 16148639 ID=cds.MS.gene033170.t1;Parent=MS.gene033170.t1
chr8.3 exon 16149245 16149810 16149245 ID=MS.gene033170.t1.exon3;Parent=MS.gene033170.t1
chr8.3 CDS 16149245 16149810 16149245 ID=cds.MS.gene033170.t1;Parent=MS.gene033170.t1
chr8.3 exon 16151259 16152177 16151259 ID=MS.gene033170.t1.exon4;Parent=MS.gene033170.t1
chr8.3 CDS 16151259 16152177 16151259 ID=cds.MS.gene033170.t1;Parent=MS.gene033170.t1
Gene Sequence

>MS.gene033170

ATGAACAAAAGAGACACATCTATGGAGAAAAGTGACAAAGAAAGTGTGGAAAACAAGCAAGTTTCAAATATCAACTATAGAGGATGGAAGGTCATGCCCTTCATCATTGGTAAGCAAGATGATTAATTATTCCATTCCATGCATGTTACTCTTATTGTTCAATATGAATTTTGAGTAAACTCATTAATTCATTTGCATATTATGCAGGAAATGAAACATTTGAGAAGCTAGGAGCCATTGGTACCTTAGCAAACCTCTTGGTCTATCTTACATCAGTCTTCAACCTTAGCAATATTGCAGCCACAAATATTGTCAATATCTTCAGTGGAAGTGCTAGTCTAGCTACCTTGCTAGGAGCTTTTCTATGTGACACTTATTTTGGACGCTACAAAACTCTTGGATTCTGCACATTTGCTTCCTTTCTGGTATTAATTATAACAATCTTAGCACTAGTTGTATTAAAATTAATACTACAATTCATCACCACAAAAAAGTAATACTTCAACAAAATTTTAAAAAAGTTTTACAATCGCGCCGTCAAGGTTGTTCAATTGTCTTACCTCGTGACCCGCAGCCATATCAGATCAGACGTATTTGATCACAATATTTTTTAATATCAAAAGTTGTTAGAATTATTTGAATATTGTTTAAGGTACGTAATTAAAGTTAGTTATTGATTAGAACCTAACTAAACTAACTAGTTAAAACTAATTTCCAACAAATCATAATTAACTTGTAACCACCTTAAACAATATTCAACTAATTCTAATAAATATTTTATTTGTGTCATGTAACTCTTTGATTTTGAAACAGGGGTTGCTTGTTATACAACTAACAGCATGGATTAAAAGCATGCATCCACCAAAATGTGGAAATGAAAGAAGCACATGCATAGAACCAAGTACAGGACAAATGTCATTTCTACTACTTGGATTTGGGCTTATGATAGTAGGAGCTGCAGGGATTAGACCTTGTAACTTAGCATTTGGAGCTGATCAATTCAATCCAAACACAGATTCAGGAAAGAAAGGAATCAATAGTTTCTTCAATTGGTATTTTTTTACCTTCACTTTTGCACAAATGGTTTCTTTGTCACTAATTGTTTATATACAAGTAAATGTGAGTTGGGCTCTTGGTCTTGGAATTCCTGCTGGTTTGATGCTGTTTTCATGTGTTATTTATTTCACTGGAAGTAAATATTATGTGAAGGTAAAAGCAACAGGTAGTCCTGTAACAAATATTGTTCAAGTGATATATGTGGCTATAAAGAAAAGGAAACTCAATCTACAAGAAGAGGATGAGTATTCATTGCTTTCTCTTTTTAGCTATATTTCTCCTTACTCTATCAACACCAAGCTTTCTCATACACCTCAGTTCAGGTAACCAACCACTAATCAATGTCCAGTATTTTTACTTCCGTATCAGAAATAATTTTTTACTATTTAATTGGCTTATGCTTATGCTTATAAAATACAATTTAATTGGCTTATTTGTTGGAGAGTTTAGAAACTGCGAGCACCAACAGGATAAATACAAGATACTTTCCCATCATTTTCTTTTTACGTCATTTTTATCCTTTTTAATCCGTTTGCATATGAAAATATGAGTGAAAGACTATAGGTTTAAACATTAAGAGCTCATATCAAAATAAAATCATAGATTTGAATCTTGAAAATTCAATTCATATACAAATTGATCAAAAGAACAAAAATGACATAAAAAAAATATAAAAGAGGAGTTTGTTACTTGAAATTAAGTTAAAGTGCGACAAAAGTAATTTTGGCAAAATCTTAAAAGACATGAATATATATGTTACTCCCTCCTTTCTTATAAGTCGCTTTGGGAAAAAATTTGTATCAAATTATAAGTCATTTTATAATACTAATGAATTATTAATGTTACTTTTTCTATTATACCTTTAACTATTTATTACTCTTTCTTTTTTCAATTCTTTAATTTATCTTTCCAATACCATTAACGAAAAATAATTTTGTAAAATTATTTATAATTTTTCATTCTAGTACACAATTGATTACATTTATTAATTTAAATGAAATAGTCAAAATGACTTATAATTTGAGACAAAGAGAGTATTTCACTTATTTATCCTTTATTTAGTTCTCTTGACGTGGGACTAACATAAGCTGTAAACATAACTATAAAGAGATCTTGAGTCATTGAGTATGTGCATTAGCTCTCTCTTATATATCATTCATTTAACTCATTCATATCAATATGATACCAACTACCGACACTTAAAATTCCAACCGATACATATTTGTGCAAGTGTTGGTTGGATAGAGCATATAAAAATAGCTCATGAAGTTTTCACTATCCATGTTCATCTTATTTGAATAAACTATTTTCAATAAACTATTTGGGCCCCAAATTCTATTTGATAATAGAATTAGCTTATATATCTGTGTGTTTATATGGTATAAACACTTATTCAATGAGTGTTTATTCTTAATTAAGTCGTTTACTCAAGCACAACCTTAACCTTAATTTGATTACAAAAATAGCTTATAGTTATATGTAAATGCTTATATGATAATCATTTGTTGAAAATAATACACGACTAAAGCGTGACCAATTGGTTCGTCGAATGAGTTTTGAATTGACTCTCATATTATCTTAGAATTTAGGTTGAATCTAATTTAACCAACAAAATCGGCTTGTATAGTGAGGATTGTCCACCACTTATTAACACATTTTTATATCATATCTCATCGAATGTGAGACTCTTTGATTTTTGCAAATACAATCTTACCATTTGTTGATTTCTTCATAAAATGATTGGTTATAAAATGTTCATATGAATTAACAGGTTCCTTGACAAAGCAGCTATTATAACCCCACAAGACAAAATAAACCCTGACGGTTCTGCATCTGATCCATGGTATCTATGCAGTATACAACAAGTGGAAGAAGTGAAATGCTTAGTAAGAGTTCTACCAATTTGGGTTTCAGGCATACTCTATTATGTTGCATTAGTCCAACAGAACACATTGTTAGTCTTCCAAGCACTTCAATCGGACCGAACCTTTTTCAACACAAATTTCAAAATCCCTGCAGCTTCTTACACGATCTTCACGATGTTAAGCCTAACGATATGGTTACCAATCTACGACAGAAAAATCGTACCAACAATGAGAAAAATCACCGGAAAAGAAGGCGGAATCACACTTCTACAAAGAATGGGAATTGGAATGTTTATTGCTATACTATGCATGTTAGTATCCGGTGTGGTAGAATGGAAAAGAAGGACCATGGCTATTAATAATCCTATAGGATTTGAACCGAGAAAAGGCGCGATATCTTCCATGTCGGGAATGTGGTTGATTCCTCAGTTAGCACTAGCAGGATTTTCTGATGCATTTACTTTGGTTGGTCAAGTTGAATTTTACTACAAACAGTTTCCTGAGAATATGAGAAGTCTTGCAGGGTCACTGTTTTTCTGTGGCTTGGCACTATCAAGTTATTTTAGTAGTTTGTTGATTACAATTATTCATAGGTTGACGGTTAAATCTGCAACAGGGAATTGGTTACCACAGGATTTGAACGAAGGGAGATTGGATTACTTTTATTACATGATTACTTGTTTGGAAGTTGTTAATTTGGGTTACTTTGTATTGTGTTCTAGGTGGTACAGGTACAAAGGGAATGATGATGGTACTGATCAAGGTAGTGGTGTTGAACTTGATCAAGTGTCTAAACAATCTGAAACAAAGATTAGTGGTGTTTAG
Protein sequence

>MS.gene033170.t1

MNKRDTSMEKSDKESVENKQVSNINYRGWKVMPFIIGNETFEKLGAIGTLANLLVYLTSVFNLSNIAATNIVNIFSGSASLATLLGAFLCDTYFGRYKTLGFCTFASFLGLLVIQLTAWIKSMHPPKCGNERSTCIEPSTGQMSFLLLGFGLMIVGAAGIRPCNLAFGADQFNPNTDSGKKGINSFFNWYFFTFTFAQMVSLSLIVYIQVNVSWALGLGIPAGLMLFSCVIYFTGSKYYVKVKATGSPVTNIVQVIYVAIKKRKLNLQEEDEYSLLSLFSYISPYSINTKLSHTPQFRFLDKAAIITPQDKINPDGSASDPWYLCSIQQVEEVKCLVRVLPIWVSGILYYVALVQQNTLLVFQALQSDRTFFNTNFKIPAASYTIFTMLSLTIWLPIYDRKIVPTMRKITGKEGGITLLQRMGIGMFIAILCMLVSGVVEWKRRTMAINNPIGFEPRKGAISSMSGMWLIPQLALAGFSDAFTLVGQVEFYYKQFPENMRSLAGSLFFCGLALSSYFSSLLITIIHRLTVKSATGNWLPQDLNEGRLDYFYYMITCLEVVNLGYFVLCSRWYRYKGNDDGTDQGSGVELDQVSKQSETKISGV