AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene034047

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034047.t1 MTR_4g011280 97.393 537 14 0 1 537 65 601 0.0 1084
MS.gene034047.t1 MTR_2g101640 71.670 533 147 2 1 529 56 588 0.0 806
MS.gene034047.t1 MTR_2g101590 71.538 520 146 1 1 518 59 578 0.0 764
MS.gene034047.t1 MTR_2g101610 70.577 520 151 1 1 518 60 579 0.0 756
MS.gene034047.t1 MTR_2g101560 68.352 534 164 2 1 529 60 593 0.0 729
MS.gene034047.t1 MTR_1g057460 67.510 514 150 3 1 514 77 573 0.0 712
MS.gene034047.t1 MTR_1g057460 67.510 514 150 3 1 514 6 502 0.0 711
MS.gene034047.t1 MTR_0236s0050 67.899 514 148 4 1 514 6 502 0.0 694
MS.gene034047.t1 MTR_2g101650 59.847 523 206 4 4 524 60 580 0.0 669
MS.gene034047.t1 MTR_8g103233 49.630 540 259 8 1 537 50 579 0.0 522
MS.gene034047.t1 MTR_1g009200 49.807 518 251 6 4 518 63 574 9.84e-170 493
MS.gene034047.t1 MTR_1g026740 48.031 508 255 5 4 507 53 555 3.94e-169 491
MS.gene034047.t1 MTR_1g026750 47.601 521 262 6 4 518 54 569 3.11e-163 476
MS.gene034047.t1 MTR_1g026770 46.796 515 264 6 4 514 51 559 1.55e-159 466
MS.gene034047.t1 MTR_8g469480 37.736 530 310 9 5 520 73 596 1.67e-122 372
MS.gene034047.t1 MTR_4g107510 37.695 512 307 9 4 505 72 581 4.41e-120 366
MS.gene034047.t1 MTR_5g038060 36.147 545 323 11 3 534 66 598 6.81e-120 366
MS.gene034047.t1 MTR_4g107580 36.452 513 313 8 4 505 60 570 1.43e-117 360
MS.gene034047.t1 MTR_5g055000 38.356 511 297 10 5 505 55 557 3.08e-117 358
MS.gene034047.t1 MTR_4g107530 35.361 526 328 9 4 519 69 592 1.68e-110 342
MS.gene034047.t1 MTR_7g088790 34.972 529 325 11 5 521 66 587 1.04e-106 332
MS.gene034047.t1 MTR_5g038190 33.981 515 324 10 1 505 57 565 2.12e-103 322
MS.gene034047.t1 MTR_8g469470 34.875 519 319 10 1 505 65 578 1.83e-101 318
MS.gene034047.t1 MTR_7g088825 31.810 547 349 13 5 535 59 597 2.32e-101 318
MS.gene034047.t1 MTR_1g009170 41.475 434 190 10 91 521 5 377 3.64e-101 310
MS.gene034047.t1 MTR_7g088820 32.276 536 344 9 5 529 62 589 7.31e-99 311
MS.gene034047.t1 MTR_7g088820 32.150 535 344 9 6 529 1 527 8.80e-99 309
MS.gene034047.t1 MTR_7g067010 35.306 490 294 8 20 499 76 552 3.66e-95 301
MS.gene034047.t1 MTR_3g462710 35.010 497 300 11 20 505 76 560 8.95e-95 300
MS.gene034047.t1 MTR_4g107610 30.965 549 346 12 1 526 64 602 3.48e-92 294
MS.gene034047.t1 MTR_4g015080 33.725 510 319 10 4 505 70 568 7.28e-91 290
MS.gene034047.t1 MTR_5g012270 31.354 539 332 12 2 521 63 582 5.57e-89 285
MS.gene034047.t1 MTR_7g088820 31.622 487 314 9 54 529 1 479 4.11e-88 280
MS.gene034047.t1 MTR_7g064780 30.451 532 350 9 3 522 61 584 1.91e-87 281
MS.gene034047.t1 MTR_7g064800 32.427 515 318 12 3 499 61 563 1.85e-86 279
MS.gene034047.t1 MTR_3g112460 32.749 513 308 14 4 502 57 546 3.39e-85 275
MS.gene034047.t1 MTR_5g085850 32.282 539 321 15 5 526 65 576 2.03e-84 273
MS.gene034047.t1 MTR_3g462710 34.125 463 282 11 54 505 1 451 2.53e-84 269
MS.gene034047.t1 MTR_4g114340 32.745 510 332 9 1 507 57 558 6.40e-84 271
MS.gene034047.t1 MTR_3g112450 33.401 491 298 10 25 505 80 551 1.25e-83 271
MS.gene034047.t1 MTR_3g072300 33.469 490 303 12 26 505 82 558 1.63e-83 271
MS.gene034047.t1 MTR_3g112470 32.681 511 311 13 4 502 57 546 1.67e-83 270
MS.gene034047.t1 MTR_4g033325 34.257 505 296 11 9 505 72 548 3.71e-83 269
MS.gene034047.t1 MTR_7g098200 32.617 512 316 11 20 523 81 571 1.33e-82 268
MS.gene034047.t1 MTR_7g098040 32.731 498 311 10 25 511 91 575 2.97e-82 268
MS.gene034047.t1 MTR_7g098200 32.177 519 319 11 20 523 81 581 3.18e-82 268
MS.gene034047.t1 MTR_5g012290 29.907 535 350 10 2 521 61 585 3.26e-82 268
MS.gene034047.t1 MTR_3g069500 31.128 514 317 11 7 505 66 557 4.98e-82 267
MS.gene034047.t1 MTR_2g093160 33.269 520 303 15 1 505 54 544 9.98e-82 265
MS.gene034047.t1 MTR_4g033315 33.803 497 295 11 23 511 86 556 1.45e-81 265
MS.gene034047.t1 MTR_4g033310 33.803 497 295 11 23 511 86 556 1.45e-81 265
MS.gene034047.t1 MTR_7g098220 33.469 490 306 11 25 505 43 521 8.61e-81 263
MS.gene034047.t1 MTR_0294s0020 33.333 495 292 11 20 505 83 548 1.88e-80 262
MS.gene034047.t1 MTR_7g098220 33.265 490 307 10 25 505 87 565 3.05e-80 263
MS.gene034047.t1 MTR_4g019580 31.890 508 320 14 20 518 88 578 3.76e-80 262
MS.gene034047.t1 MTR_7g098180 33.814 485 308 9 24 502 87 564 3.92e-80 262
MS.gene034047.t1 MTR_7g098230 32.422 512 329 11 19 523 77 578 6.93e-80 261
MS.gene034047.t1 MTR_2g093180 33.911 519 296 14 4 505 57 545 2.80e-79 259
MS.gene034047.t1 MTR_4g101380 30.654 535 349 11 2 521 61 588 5.93e-79 259
MS.gene034047.t1 MTR_2g093640 33.723 513 304 11 4 505 57 544 2.38e-78 256
MS.gene034047.t1 MTR_7g098160 31.412 503 327 10 25 519 90 582 2.70e-78 258
MS.gene034047.t1 MTR_7g065080 30.814 516 320 13 6 505 61 555 4.33e-78 256
MS.gene034047.t1 MTR_3g069420 32.258 496 313 11 19 505 85 566 5.46e-78 256
MS.gene034047.t1 MTR_3g005010 31.727 498 299 9 20 509 82 546 1.13e-77 254
MS.gene034047.t1 MTR_3g069440 30.485 515 329 10 7 505 64 565 2.10e-77 255
MS.gene034047.t1 MTR_7g065080 31.084 489 308 12 25 505 1 468 2.15e-77 252
MS.gene034047.t1 MTR_1g075440 31.845 515 327 10 19 522 79 580 2.69e-77 254
MS.gene034047.t1 MTR_1g042310 31.081 518 327 12 19 522 33 534 3.97e-77 253
MS.gene034047.t1 MTR_4g033325 33.689 469 275 11 45 505 10 450 3.98e-77 250
MS.gene034047.t1 MTR_1g042310 31.081 518 327 12 19 522 40 541 5.27e-77 253
MS.gene034047.t1 MTR_4g033330 34.316 475 280 11 20 488 83 531 1.08e-76 251
MS.gene034047.t1 MTR_1g042310 31.081 518 327 12 19 522 77 578 1.09e-76 253
MS.gene034047.t1 MTR_7g098090 31.456 515 316 11 25 519 90 587 1.59e-76 253
MS.gene034047.t1 MTR_7g006540 32.752 516 306 11 18 505 107 609 1.84e-76 253
MS.gene034047.t1 MTR_4g033325 33.710 442 266 10 72 505 2 424 1.27e-75 246
MS.gene034047.t1 MTR_1g041885 31.875 480 298 13 19 488 33 493 2.31e-75 248
MS.gene034047.t1 MTR_2g005130 29.730 518 333 12 8 517 80 574 1.24e-74 248
MS.gene034047.t1 MTR_1g041875 31.969 513 322 12 19 522 80 574 1.55e-74 247
MS.gene034047.t1 MTR_3g100980 31.784 538 332 14 2 525 50 566 1.59e-74 247
MS.gene034047.t1 MTR_5g014150 29.528 508 346 7 20 519 96 599 1.84e-74 248
MS.gene034047.t1 MTR_2g017750 28.864 537 357 14 1 518 67 597 9.38e-74 246
MS.gene034047.t1 MTR_8g038880 31.200 500 296 10 19 499 71 541 2.02e-73 244
MS.gene034047.t1 MTR_4g033385 32.974 464 272 10 53 505 16 451 1.19e-72 239
MS.gene034047.t1 MTR_7g098200 31.340 485 300 11 54 523 1 467 3.51e-72 238
MS.gene034047.t1 MTR_1g085090 28.177 543 361 13 1 535 52 573 8.93e-72 240
MS.gene034047.t1 MTR_1g041815 30.019 513 338 10 19 522 78 578 6.26e-70 235
MS.gene034047.t1 MTR_7g005930 30.019 523 340 10 9 521 76 582 1.63e-69 234
MS.gene034047.t1 MTR_1g041835 29.447 506 336 10 26 522 85 578 1.86e-69 233
MS.gene034047.t1 MTR_4g033375 32.955 440 264 9 20 453 84 498 2.19e-69 231
MS.gene034047.t1 MTR_4g033395 33.700 454 264 9 9 453 73 498 2.99e-69 231
MS.gene034047.t1 MTR_3g100980 30.389 566 331 15 2 525 50 594 5.13e-69 233
MS.gene034047.t1 MTR_4g098800 29.757 494 324 10 20 502 88 569 3.27e-68 231
MS.gene034047.t1 MTR_4g098800 29.757 494 324 10 20 502 153 634 1.04e-67 231
MS.gene034047.t1 MTR_3g014790 30.485 515 307 13 26 522 75 556 1.74e-67 228
MS.gene034047.t1 MTR_8g469310 31.753 485 284 15 45 504 103 565 5.49e-66 224
MS.gene034047.t1 MTR_7g010820 30.608 477 314 10 20 485 78 548 2.85e-65 223
MS.gene034047.t1 MTR_8g087780 30.723 498 320 14 1 479 54 545 1.43e-64 221
MS.gene034047.t1 MTR_7g111260 29.070 516 326 15 19 524 70 555 4.92e-64 219
MS.gene034047.t1 MTR_7g013270 30.502 518 330 10 20 522 142 644 9.82e-64 220
MS.gene034047.t1 MTR_8g087810 30.566 530 343 12 1 508 54 580 1.09e-63 218
MS.gene034047.t1 MTR_1g116930 29.045 513 333 13 20 522 75 566 9.22e-63 216
MS.gene034047.t1 MTR_5g038380 28.226 496 312 11 33 504 93 568 1.62e-61 213
MS.gene034047.t1 MTR_4g107670 29.554 538 318 18 6 505 54 568 8.30e-61 210
MS.gene034047.t1 MTR_4g123537 28.291 509 328 15 25 522 98 580 1.91e-54 194
MS.gene034047.t1 MTR_6g008690 29.330 433 261 12 99 508 127 537 6.82e-54 191
MS.gene034047.t1 MTR_7g098210 27.713 516 281 13 6 511 12 445 4.79e-52 184
MS.gene034047.t1 MTR_4g033310 31.024 332 212 5 184 511 174 492 2.34e-51 183
MS.gene034047.t1 MTR_4g033310 41.593 113 53 4 23 134 86 186 5.29e-12 68.6
MS.gene034047.t1 MTR_7g098150 29.945 364 242 8 25 383 90 445 3.80e-47 171
MS.gene034047.t1 MTR_4g024930 27.510 498 318 14 19 505 87 552 6.41e-44 164
MS.gene034047.t1 MTR_7g052600 25.000 492 322 11 19 505 76 525 2.11e-36 142
MS.gene034047.t1 MTR_7g051880 25.720 486 314 12 19 499 76 519 1.78e-34 137
MS.gene034047.t1 MTR_7g051830 24.897 486 318 11 19 499 76 519 9.41e-33 132
MS.gene034047.t1 MTR_8g091265 39.450 109 60 4 235 339 3 109 1.05e-17 80.5
MS.gene034047.t1 MTR_3g065350 28.485 165 108 5 277 439 1 157 3.30e-14 70.9
MS.gene034047.t1 MTR_4g033390 34.043 94 59 2 423 513 30 123 5.38e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene034047.t1 AT1G52190 54.696 543 236 6 4 536 57 599 0.0 632
MS.gene034047.t1 AT3G16180 54.664 536 230 7 1 524 56 590 0.0 617
MS.gene034047.t1 AT1G69870 36.447 546 315 12 1 522 76 613 5.44e-120 367
MS.gene034047.t1 AT1G68570 36.773 533 320 9 5 524 30 558 1.98e-110 340
MS.gene034047.t1 AT1G68570 36.773 533 320 9 5 524 60 588 7.07e-110 340
MS.gene034047.t1 AT5G62680 34.962 532 330 10 4 522 79 607 1.88e-108 337
MS.gene034047.t1 AT3G47960 34.961 512 320 8 4 505 97 605 7.85e-104 325
MS.gene034047.t1 AT1G27080 34.011 541 331 13 4 535 8 531 4.12e-103 320
MS.gene034047.t1 AT1G27080 34.011 541 331 13 4 535 50 573 1.12e-102 320
MS.gene034047.t1 AT1G18880 34.047 514 322 10 4 505 55 563 1.56e-100 315
MS.gene034047.t1 AT1G62200 37.081 507 290 12 7 501 92 581 2.86e-98 310
MS.gene034047.t1 AT3G54140 34.818 494 305 8 20 505 76 560 4.54e-97 306
MS.gene034047.t1 AT3G54140 34.818 494 305 8 20 505 76 560 4.54e-97 306
MS.gene034047.t1 AT2G02040 35.060 502 305 10 7 498 81 571 8.63e-96 303
MS.gene034047.t1 AT1G62200 35.888 535 287 14 7 501 92 610 7.20e-94 299
MS.gene034047.t1 AT5G28470 31.703 511 322 10 1 498 50 546 8.75e-94 297
MS.gene034047.t1 AT1G62200 35.888 535 287 14 7 501 106 624 1.20e-93 299
MS.gene034047.t1 AT5G01180 34.490 490 296 12 26 503 83 559 1.08e-91 292
MS.gene034047.t1 AT5G01180 34.490 490 296 12 26 503 83 559 1.08e-91 292
MS.gene034047.t1 AT5G01180 34.490 490 296 12 26 503 83 559 1.08e-91 292
MS.gene034047.t1 AT5G28470 31.507 511 321 11 1 498 50 544 7.48e-91 289
MS.gene034047.t1 AT5G11570 37.402 508 234 15 4 506 49 477 7.97e-91 287
MS.gene034047.t1 AT3G45650 32.871 505 314 9 4 505 58 540 2.37e-90 288
MS.gene034047.t1 AT2G26690 33.936 498 315 10 33 526 44 531 3.24e-90 287
MS.gene034047.t1 AT2G26690 33.936 498 315 10 33 526 56 543 5.76e-90 286
MS.gene034047.t1 AT2G26690 33.396 530 339 10 1 526 57 576 1.68e-89 286
MS.gene034047.t1 AT3G21670 32.348 541 331 13 5 532 64 582 1.80e-85 276
MS.gene034047.t1 AT3G45660 32.742 507 314 11 3 505 56 539 9.15e-85 273
MS.gene034047.t1 AT3G45660 32.742 507 314 11 3 505 56 539 9.15e-85 273
MS.gene034047.t1 AT5G46040 32.696 523 327 8 19 530 78 586 1.06e-84 274
MS.gene034047.t1 AT3G45650 31.262 531 314 10 4 505 58 566 1.26e-84 274
MS.gene034047.t1 AT1G69860 33.528 513 306 12 7 505 55 546 1.34e-84 273
MS.gene034047.t1 AT3G45660 32.544 507 315 11 3 505 77 560 2.03e-84 273
MS.gene034047.t1 AT2G02020 32.347 507 293 11 4 505 79 540 7.38e-83 268
MS.gene034047.t1 AT3G45720 29.924 528 332 12 3 523 54 550 9.93e-82 265
MS.gene034047.t1 AT3G45710 31.496 508 313 11 23 523 76 555 2.46e-80 262
MS.gene034047.t1 AT1G33440 32.830 530 320 12 8 522 78 586 8.64e-80 261
MS.gene034047.t1 AT5G46050 31.068 515 330 8 19 522 78 578 1.88e-79 260
MS.gene034047.t1 AT3G54450 29.787 517 323 10 19 519 63 555 3.53e-79 259
MS.gene034047.t1 AT3G45680 31.120 527 329 11 3 523 55 553 5.26e-79 258
MS.gene034047.t1 AT1G59740 32.000 525 328 14 8 519 81 589 4.33e-78 257
MS.gene034047.t1 AT2G40460 31.600 500 311 11 26 514 82 561 6.76e-77 253
MS.gene034047.t1 AT1G12110 32.402 537 336 13 5 524 62 588 7.07e-77 253
MS.gene034047.t1 AT5G13400 31.792 519 331 9 18 521 108 618 2.57e-76 253
MS.gene034047.t1 AT1G22540 31.224 490 309 8 21 505 83 549 5.54e-74 245
MS.gene034047.t1 AT3G53960 29.091 495 325 11 22 505 91 570 9.42e-74 246
MS.gene034047.t1 AT3G45700 29.533 535 332 11 3 529 51 548 3.38e-73 243
MS.gene034047.t1 AT1G72125 30.081 492 315 9 22 505 83 553 3.82e-73 243
MS.gene034047.t1 AT1G72140 31.014 503 297 9 17 505 83 549 5.50e-73 242
MS.gene034047.t1 AT2G37900 29.652 489 316 11 22 502 13 481 6.59e-73 241
MS.gene034047.t1 AT2G37900 29.652 489 316 11 22 502 92 560 3.75e-72 241
MS.gene034047.t1 AT5G46050 30.833 480 307 8 54 522 1 466 1.05e-71 236
MS.gene034047.t1 AT1G32450 27.925 530 362 9 20 537 89 610 1.71e-70 237
MS.gene034047.t1 AT1G27040 30.290 482 306 10 34 504 61 523 9.69e-70 233
MS.gene034047.t1 AT1G27040 30.290 482 306 10 34 504 93 555 2.78e-69 233
MS.gene034047.t1 AT1G27040 30.290 482 306 10 34 504 89 551 3.20e-69 233
MS.gene034047.t1 AT1G22570 29.897 485 294 9 20 486 45 501 1.11e-66 225
MS.gene034047.t1 AT1G22570 29.897 485 294 9 20 486 82 538 2.16e-66 225
MS.gene034047.t1 AT2G02020 30.693 505 283 12 4 505 79 519 2.33e-65 221
MS.gene034047.t1 AT1G22550 29.447 506 306 12 20 505 82 556 3.36e-65 222
MS.gene034047.t1 AT4G21680 29.563 504 324 11 20 505 80 570 7.11e-65 221
MS.gene034047.t1 AT5G14940 29.183 514 324 13 22 518 61 551 5.19e-64 218
MS.gene034047.t1 AT1G72120 30.346 491 309 9 23 505 84 549 1.01e-63 218
MS.gene034047.t1 AT3G45690 29.857 489 272 14 23 505 73 496 2.09e-62 213
MS.gene034047.t1 AT1G72130 28.889 495 302 10 20 505 77 530 1.04e-61 212
MS.gene034047.t1 AT5G19640 27.866 506 330 10 20 515 111 591 3.94e-60 209
MS.gene034047.t1 AT3G01350 29.533 535 311 18 22 523 61 562 6.73e-60 207
MS.gene034047.t1 AT1G69850 28.717 491 324 11 33 504 90 573 9.23e-59 205
MS.gene034047.t1 AT3G25260 27.593 511 307 13 3 504 56 512 1.22e-54 192
MS.gene034047.t1 AT1G72130 28.605 430 267 9 85 505 14 412 8.84e-52 182
MS.gene034047.t1 AT1G72130 28.605 430 267 9 85 505 14 412 8.84e-52 182
MS.gene034047.t1 AT3G25280 26.772 508 325 12 1 504 54 518 1.40e-48 176
MS.gene034047.t1 AT5G62730 27.475 495 328 11 19 504 48 520 3.50e-48 175
MS.gene034047.t1 AT5G62730 27.475 495 328 11 19 504 107 579 8.45e-48 175
MS.gene034047.t1 AT2G38100 23.669 507 330 21 31 522 37 501 1.51e-20 95.5
MS.gene034047.t1 AT2G38100 23.866 507 329 21 31 522 59 523 3.11e-20 94.4
MS.gene034047.t1 AT2G38100 23.669 507 330 21 31 522 53 517 3.64e-20 94.4

Find 114 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATTTCATGCCTCTTAT+TGG 0.106772 4.4:-88180612 MS.gene034047:CDS
TCCAATGCCCTTTGGTTATC+AGG 0.181681 4.4:+88179499 None:intergenic
CAGCCGGAACTTCGAAGTTT+TGG 0.192354 4.4:+88178783 None:intergenic
CGGTATAGTAGAACTCATTT+TGG 0.208877 4.4:+88178450 None:intergenic
GCCGATTCGACAAGTATTAT+TGG 0.244280 4.4:-88178274 MS.gene034047:CDS
TCATTTCTGAAGTCAATTAA+TGG 0.244897 4.4:+88178140 None:intergenic
AGGTTTCTTGCTGTTGGTTT+AGG 0.258775 4.4:-88180561 MS.gene034047:CDS
CTTAACATTGGAGGCTCATT+TGG 0.269927 4.4:-88178842 MS.gene034047:CDS
CTGTTCCAACTGATAAAATA+AGG 0.273173 4.4:-88179174 MS.gene034047:intron
GCTGAAGCATTCAATGTTAT+AGG 0.290021 4.4:-88178473 MS.gene034047:CDS
TGATATTCACAATATTTATA+TGG 0.291881 4.4:-88178742 MS.gene034047:CDS
CTTGGATGGAAACTTGGTTT+TGG 0.310808 4.4:-88179394 MS.gene034047:CDS
ACAAATAGCACCTTGTTTAC+CGG 0.313397 4.4:-88179298 MS.gene034047:CDS
TTCATTGCTGATTCTCATCT+TGG 0.318778 4.4:-88180585 MS.gene034047:CDS
GTAGTATGTAGTTGGGCTTA+TGG 0.330481 4.4:-88178212 MS.gene034047:CDS
ACCAATAAGAGGCATGAAAT+TGG 0.330763 4.4:+88180611 None:intergenic
AGGTTCTATCATCACTTTCA+TGG 0.335587 4.4:-88180541 MS.gene034047:intron
TGGTGGTTGCAACAGATAAA+AGG 0.339836 4.4:+88180631 None:intergenic
TCCCTTGCTCTTATGTCCAT+TGG 0.348657 4.4:-88179583 MS.gene034047:CDS
CTTCAATGCTCCATAGCGTT+TGG 0.357999 4.4:-88179550 MS.gene034047:CDS
CAGCAATTGTCGAGACTATA+AGG 0.361104 4.4:-88178595 MS.gene034047:CDS
CTTGGAAGGTTTCTTGCTGT+TGG 0.361872 4.4:-88180567 MS.gene034047:CDS
TACATTCCAGCCGAGTTCTT+AGG 0.374613 4.4:+88179223 None:intergenic
AACCCAAAACTTCGAAGTTC+CGG 0.380968 4.4:-88178786 MS.gene034047:CDS
TGGAAATGATGGATGGATAT+CGG 0.386093 4.4:-88178312 MS.gene034047:CDS
TTCTGTCATAATTGCATTCA+CGG 0.389746 4.4:-88179440 MS.gene034047:CDS
GAAAAGAACATAAGTGTTGC+TGG 0.403449 4.4:+88179367 None:intergenic
GTTTCCATCCAAGATGATCT+TGG 0.407018 4.4:+88179404 None:intergenic
TGGAATGTATCATCATATAA+AGG 0.408219 4.4:-88179209 MS.gene034047:CDS
CGACATAGTCTTGGGAAATT+CGG 0.414520 4.4:+88178430 None:intergenic
GTCTTTGCACTAATCCTAAC+CGG 0.414813 4.4:+88178665 None:intergenic
TGTATTATGTAGTATGTAGT+TGG 0.415728 4.4:-88178220 MS.gene034047:CDS
AGGTCTTGCTTGTGGGATCA+TGG 0.416917 4.4:+88179675 None:intergenic
GCCAATAATACTTGTCGAAT+CGG 0.421090 4.4:+88178273 None:intergenic
AGAGCAATCAATGAAGGATA+TGG 0.424291 4.4:-88178566 MS.gene034047:CDS
GATCATCTTGGATGGAAACT+TGG 0.426344 4.4:-88179400 MS.gene034047:CDS
ATGGATCAGCTTCAGATCCT+TGG 0.436157 4.4:-88178949 MS.gene034047:CDS
TTTCCTCAACTTTGCTTGGG+TGG 0.439391 4.4:-88178500 MS.gene034047:CDS
GCTCTTATGTCCATTGGAAA+TGG 0.445474 4.4:-88179577 MS.gene034047:CDS
AGAATATTTCCAATGCCCTT+TGG 0.446255 4.4:+88179491 None:intergenic
GCAATGAAAGCACCAATAAG+AGG 0.457008 4.4:+88180600 None:intergenic
CTCGAAGCTCAGAGGAAAAC+CGG 0.457609 4.4:-88178684 MS.gene034047:CDS
ATCTGCAACAACTTCACAAA+TGG 0.461858 4.4:-88179620 MS.gene034047:CDS
ATTAATTGACTTCAGAAATG+AGG 0.462266 4.4:-88178138 MS.gene034047:CDS
CCCTGATAACCAAAGGGCAT+TGG 0.463599 4.4:-88179500 MS.gene034047:CDS
TTGCTGATTCTCATCTTGGA+AGG 0.464292 4.4:-88180581 MS.gene034047:CDS
ACCTCGAGAGGTGGAAATGA+TGG 0.466678 4.4:-88178323 MS.gene034047:CDS
AAGGAGGAGGTCTTGCTTGT+GGG 0.467412 4.4:+88179668 None:intergenic
TTTATTCAGCATTGTCGAAA+GGG 0.476280 4.4:-88178348 MS.gene034047:CDS
ATTAGTGCAAAGACAAGAAT+GGG 0.480477 4.4:-88178656 MS.gene034047:CDS
CAAACGCTATGGAGCATTGA+AGG 0.485367 4.4:+88179551 None:intergenic
GTATTATGTAGTATGTAGTT+GGG 0.485998 4.4:-88178219 MS.gene034047:CDS
ATCAAAAGTAAGTGAAGATG+AGG 0.488823 4.4:-88178171 MS.gene034047:CDS
CCAATGCCCTTTGGTTATCA+GGG 0.489270 4.4:+88179500 None:intergenic
CTGGTCCGCGCCAAACGCTA+TGG 0.494260 4.4:+88179540 None:intergenic
CATAGCGTTTGGCGCGGACC+AGG 0.495367 4.4:-88179539 MS.gene034047:CDS
CATTGGAAATATTCTTCAGT+TGG 0.496292 4.4:-88179483 MS.gene034047:CDS
CCGTTGCGGCTTATAAGAAC+AGG 0.500334 4.4:-88179261 MS.gene034047:CDS
TGGCTTATTGCCACAGTGAG+TGG 0.505821 4.4:-88178254 MS.gene034047:CDS
AGCGATGCTCGACATAGTCT+TGG 0.506861 4.4:+88178421 None:intergenic
GATTAGTGCAAAGACAAGAA+TGG 0.507012 4.4:-88178657 MS.gene034047:CDS
TCTGTCATAATTGCATTCAC+GGG 0.521279 4.4:-88179439 MS.gene034047:CDS
TTGATGTCACTTAACATTGG+AGG 0.525633 4.4:-88178851 MS.gene034047:CDS
GCGATGCTCGACATAGTCTT+GGG 0.526210 4.4:+88178422 None:intergenic
CAGTTGGTACTATGCTTCCT+CGG 0.529829 4.4:-88179467 MS.gene034047:CDS
CGAAGAAGAGCAATCAATGA+AGG 0.534308 4.4:-88178572 MS.gene034047:CDS
TTAATTGACTTCAGAAATGA+GGG 0.534447 4.4:-88178137 MS.gene034047:CDS
CAAGGAGGAGGTCTTGCTTG+TGG 0.537281 4.4:+88179667 None:intergenic
GACATGTTCAACACTGAATG+AGG 0.541800 4.4:+88178536 None:intergenic
CTACCACCTAAGAACTCGGC+TGG 0.541857 4.4:-88179229 MS.gene034047:CDS
GTATATATCCAAGATCATCT+TGG 0.542980 4.4:-88179412 MS.gene034047:CDS
CAAAACTTCGAAGTTCCGGC+TGG 0.549745 4.4:-88178782 MS.gene034047:CDS
GCAAAGACAAGAATGGGACT+TGG 0.551458 4.4:-88178650 MS.gene034047:CDS
AGAAGATATAATGTATCCTA+CGG 0.552827 4.4:+88178373 None:intergenic
AGCTCAGAGGAAAACCGGTT+AGG 0.555051 4.4:-88178679 MS.gene034047:CDS
TTTGATTCTACATCAGCAGT+AGG 0.565843 4.4:+88178188 None:intergenic
CTTATGTCCATTGGAAATGG+TGG 0.567721 4.4:-88179574 MS.gene034047:CDS
TTGAAGGCCACCATTTCCAA+TGG 0.569645 4.4:+88179567 None:intergenic
TCGTACAAAGCCGGTAAACA+AGG 0.571512 4.4:+88179288 None:intergenic
CCTCTGAGCTTCGAGGCTAG+AGG 0.574416 4.4:+88178692 None:intergenic
CGAGAGGTGGAAATGATGGA+TGG 0.575410 4.4:-88178319 MS.gene034047:CDS
AACGGTCACTCGTACAAAGC+CGG 0.581357 4.4:+88179279 None:intergenic
CCTGTTCTTATAAGCCGCAA+CGG 0.581934 4.4:+88179261 None:intergenic
AGATAACCCTGATAACCAAA+GGG 0.582920 4.4:-88179506 MS.gene034047:CDS
TCCATCATTTCCACCTCTCG+AGG 0.582933 4.4:+88178322 None:intergenic
ATATCCAAGATCATCTTGGA+TGG 0.583794 4.4:-88179408 MS.gene034047:CDS
TCAATTGTGCATAGACTCCA+AGG 0.586697 4.4:+88178932 None:intergenic
AAGATAACCCTGATAACCAA+AGG 0.589458 4.4:-88179507 MS.gene034047:CDS
TCCAATGGACATAAGAGCAA+GGG 0.589995 4.4:+88179582 None:intergenic
AATAAGAGGCATGAAATTGG+TGG 0.602647 4.4:+88180614 None:intergenic
GTTGCAGGAATGCAAGGAGG+AGG 0.607647 4.4:+88179655 None:intergenic
AACAGGGAATGATACTCTTG+TGG 0.610637 4.4:-88179705 MS.gene034047:intron
ATCTTGATGTCACTTAACAT+TGG 0.611897 4.4:-88178854 MS.gene034047:CDS
TTCCAATGGACATAAGAGCA+AGG 0.616534 4.4:+88179581 None:intergenic
TCGACTACCACCTAAGAACT+CGG 0.619351 4.4:-88179233 MS.gene034047:CDS
ATTCCACCCAAGCAAAGTTG+AGG 0.630367 4.4:+88178497 None:intergenic
ATTCCAGCCGAGTTCTTAGG+TGG 0.637012 4.4:+88179226 None:intergenic
AAATGAGGGTTCATATGACA+AGG 0.644999 4.4:-88178123 MS.gene034047:CDS
GAAAGGGTAACCTCGAGAGG+TGG 0.651879 4.4:-88178332 MS.gene034047:CDS
ATGCTCCATAGCGTTTGGCG+CGG 0.653136 4.4:-88179545 MS.gene034047:CDS
ATCTGAAGCTGATCCATCAG+AGG 0.653350 4.4:+88178955 None:intergenic
GTCGAAAGGGTAACCTCGAG+AGG 0.656180 4.4:-88178335 MS.gene034047:CDS
CTTTGTACGAGTGACCGTTG+CGG 0.660158 4.4:-88179275 MS.gene034047:CDS
GATATCGGATAATATTAACA+AGG 0.662248 4.4:-88178297 MS.gene034047:CDS
CCTCTAGCCTCGAAGCTCAG+AGG 0.662495 4.4:-88178692 MS.gene034047:CDS
TCATTAAAGTTATTCCACTG+TGG 0.683505 4.4:-88178886 MS.gene034047:CDS
ATATCGGATAATATTAACAA+GGG 0.683833 4.4:-88178296 MS.gene034047:CDS
TCAGTTGCAGGAATGCAAGG+AGG 0.690858 4.4:+88179652 None:intergenic
ATTACACTAAGTGAACCAGC+CGG 0.696488 4.4:+88178767 None:intergenic
AATTATGACAGAAATAGCCG+AGG 0.702109 4.4:+88179450 None:intergenic
TTCGGACTAGGAATGGCCGT+AGG 0.705285 4.4:-88178389 MS.gene034047:CDS
TATATTAAGACCACTCACTG+TGG 0.737154 4.4:+88178244 None:intergenic
TGTTGAACATGTCTGCAATG+TGG 0.741046 4.4:-88178526 MS.gene034047:CDS
AAGATGCTTGTAGACCACAG+TGG 0.801613 4.4:+88178872 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATATTCACAATATTTATA+TGG - chr4.4:88180048-88180067 MS.gene034047:intron 15.0%
!! AATATAACATGAAAGTTTAG+AGG - chr4.4:88178650-88178669 MS.gene034047:CDS 20.0%
!! ACAAAATCATTTACATTGTT+TGG + chr4.4:88179032-88179051 None:intergenic 20.0%
!! ATATCAACTTATCTAAATGT+AGG + chr4.4:88179678-88179697 None:intergenic 20.0%
!! ATATCGGATAATATTAACAA+GGG - chr4.4:88180494-88180513 MS.gene034047:intron 20.0%
!! CCAAATATTAAATAAGTATC+TGG + chr4.4:88178770-88178789 None:intergenic 20.0%
!! CCAGATACTTATTTAATATT+TGG - chr4.4:88178767-88178786 MS.gene034047:CDS 20.0%
!! GTATTAATATACTGAATTTG+TGG - chr4.4:88179747-88179766 MS.gene034047:intron 20.0%
!! TATCAACTTATCTAAATGTA+GGG + chr4.4:88179677-88179696 None:intergenic 20.0%
!!! GTTTTATTCTTTACATAAAG+TGG + chr4.4:88179474-88179493 None:intergenic 20.0%
! AGAAGATATAATGTATCCTA+CGG + chr4.4:88180420-88180439 None:intergenic 25.0%
! CAAATAAAGATGATGCATTT+AGG - chr4.4:88178730-88178749 MS.gene034047:CDS 25.0%
! CACAAAATGACAACAAAAAT+TGG + chr4.4:88179712-88179731 None:intergenic 25.0%
! GATATCGGATAATATTAACA+AGG - chr4.4:88180493-88180512 MS.gene034047:intron 25.0%
! GTATTATGTAGTATGTAGTT+GGG - chr4.4:88180571-88180590 MS.gene034047:CDS 25.0%
! TAAAGAGCTTCTTTAATAAC+AGG - chr4.4:88178796-88178815 MS.gene034047:CDS 25.0%
! TGGAATGTATCATCATATAA+AGG - chr4.4:88179581-88179600 MS.gene034047:CDS 25.0%
! TGTATTATGTAGTATGTAGT+TGG - chr4.4:88180570-88180589 MS.gene034047:CDS 25.0%
!! ATCATTCACATGATAATGAT+AGG + chr4.4:88178298-88178317 None:intergenic 25.0%
!! ATTAATTGACTTCAGAAATG+AGG - chr4.4:88180652-88180671 MS.gene034047:CDS 25.0%
!! TTAATTGACTTCAGAAATGA+GGG - chr4.4:88180653-88180672 MS.gene034047:CDS 25.0%
!!! ACACTAGACATTTTAGAAAA+TGG - chr4.4:88178387-88178406 MS.gene034047:CDS 25.0%
!!! TCATTTCTGAAGTCAATTAA+TGG + chr4.4:88180653-88180672 None:intergenic 25.0%
!!! TTCAACCTTATTTTATCAGT+TGG + chr4.4:88179624-88179643 None:intergenic 25.0%
AAAATGTCTAGTGTTTGTGT+CGG + chr4.4:88178381-88178400 None:intergenic 30.0%
ACATTGTTTGGTACAAATGA+GGG + chr4.4:88179020-88179039 None:intergenic 30.0%
AGCAAGAAAAAAGAAGAAAG+TGG + chr4.4:88179448-88179467 None:intergenic 30.0%
ATCAAAAGTAAGTGAAGATG+AGG - chr4.4:88180619-88180638 MS.gene034047:CDS 30.0%
ATTAGTGCAAAGACAAGAAT+GGG - chr4.4:88180134-88180153 MS.gene034047:intron 30.0%
CATTGGAAATATTCTTCAGT+TGG - chr4.4:88179307-88179326 MS.gene034047:CDS 30.0%
CATTGTGAAGACAAGATTAA+TGG - chr4.4:88178489-88178508 MS.gene034047:CDS 30.0%
CTAATATTGTGAGATTTCGA+CGG - chr4.4:88178586-88178605 MS.gene034047:CDS 30.0%
CTGTTCCAACTGATAAAATA+AGG - chr4.4:88179616-88179635 MS.gene034047:CDS 30.0%
GAAATCTCACAATATTAGAG+AGG + chr4.4:88178584-88178603 None:intergenic 30.0%
GTATATATCCAAGATCATCT+TGG - chr4.4:88179378-88179397 MS.gene034047:CDS 30.0%
TAATATTGTGAGATTTCGAC+GGG - chr4.4:88178587-88178606 MS.gene034047:CDS 30.0%
TACATTGTTTGGTACAAATG+AGG + chr4.4:88179021-88179040 None:intergenic 30.0%
TCATTAAAGTTATTCCACTG+TGG - chr4.4:88179904-88179923 MS.gene034047:intron 30.0%
TGTCTTCACAATGAAACAAT+TGG + chr4.4:88178482-88178501 None:intergenic 30.0%
TTCAATGAATCTTTCTTGCT+TGG - chr4.4:88178528-88178547 MS.gene034047:CDS 30.0%
TTCTGTCATAATTGCATTCA+CGG - chr4.4:88179350-88179369 MS.gene034047:CDS 30.0%
TTTATTCAGCATTGTCGAAA+GGG - chr4.4:88180442-88180461 MS.gene034047:intron 30.0%
! ATCTTGATGTCACTTAACAT+TGG - chr4.4:88179936-88179955 MS.gene034047:intron 30.0%
! CAACTCAATTTTCTTCCTTT+TGG - chr4.4:88179061-88179080 MS.gene034047:intron 30.0%
! GTTTTTCTCTTTTCTTCACT+TGG - chr4.4:88180166-88180185 MS.gene034047:intron 30.0%
! TTTTATTCAGCATTGTCGAA+AGG - chr4.4:88180441-88180460 MS.gene034047:intron 30.0%
!! ATGTGAATGATTAACTTGTG+TGG - chr4.4:88178307-88178326 MS.gene034047:CDS 30.0%
!! ATTTTCTTCCTTTTGGTAAC+AGG - chr4.4:88179068-88179087 MS.gene034047:intron 30.0%
!! TTTTCTTCCTTTTGGTAACA+GGG - chr4.4:88179069-88179088 MS.gene034047:intron 30.0%
AAATGAGGGTTCATATGACA+AGG - chr4.4:88180667-88180686 MS.gene034047:CDS 35.0%
AAGATAACCCTGATAACCAA+AGG - chr4.4:88179283-88179302 MS.gene034047:CDS 35.0%
AATTATGACAGAAATAGCCG+AGG + chr4.4:88179343-88179362 None:intergenic 35.0%
ACCAATTTCATGCCTCTTAT+TGG - chr4.4:88178178-88178197 MS.gene034047:CDS 35.0%
AGAATATTTCCAATGCCCTT+TGG + chr4.4:88179302-88179321 None:intergenic 35.0%
AGAGCAATCAATGAAGGATA+TGG - chr4.4:88180224-88180243 MS.gene034047:intron 35.0%
AGATAACCCTGATAACCAAA+GGG - chr4.4:88179284-88179303 MS.gene034047:CDS 35.0%
AGGTTCTATCATCACTTTCA+TGG - chr4.4:88178249-88178268 MS.gene034047:CDS 35.0%
ATATCCAAGATCATCTTGGA+TGG - chr4.4:88179382-88179401 MS.gene034047:CDS 35.0%
ATATTGACACCAACACGATA+CGG + chr4.4:88178427-88178446 None:intergenic 35.0%
ATCTGCAACAACTTCACAAA+TGG - chr4.4:88179170-88179189 MS.gene034047:intron 35.0%
GAAAAAGACATAACCTCTGA+TGG - chr4.4:88179822-88179841 MS.gene034047:intron 35.0%
GATTAGTGCAAAGACAAGAA+TGG - chr4.4:88180133-88180152 MS.gene034047:intron 35.0%
GCCAATAATACTTGTCGAAT+CGG + chr4.4:88180520-88180539 None:intergenic 35.0%
GTGAAGACAAGATTAATGGA+GGG - chr4.4:88178493-88178512 MS.gene034047:CDS 35.0%
TATATTAAGACCACTCACTG+TGG + chr4.4:88180549-88180568 None:intergenic 35.0%
TATCATTCCCTGTTACCAAA+AGG + chr4.4:88179079-88179098 None:intergenic 35.0%
TCTGTCATAATTGCATTCAC+GGG - chr4.4:88179351-88179370 MS.gene034047:CDS 35.0%
TGAAGACAAGATTAATGGAG+GGG - chr4.4:88178494-88178513 MS.gene034047:CDS 35.0%
TGTGAAGACAAGATTAATGG+AGG - chr4.4:88178492-88178511 MS.gene034047:CDS 35.0%
TTTGATTCTACATCAGCAGT+AGG + chr4.4:88180605-88180624 None:intergenic 35.0%
! AATAAGAGGCATGAAATTGG+TGG + chr4.4:88178179-88178198 None:intergenic 35.0%
! ACAAATAGCACCTTGTTTAC+CGG - chr4.4:88179492-88179511 MS.gene034047:CDS 35.0%
! ACCAATAAGAGGCATGAAAT+TGG + chr4.4:88178182-88178201 None:intergenic 35.0%
! CATCTTTATTTGATTCACCG+AGG + chr4.4:88178722-88178741 None:intergenic 35.0%
! CGGTATAGTAGAACTCATTT+TGG + chr4.4:88180343-88180362 None:intergenic 35.0%
! CTTTTTCCTCAACTTTGCTT+GGG - chr4.4:88180287-88180306 MS.gene034047:intron 35.0%
! GAAAAGAACATAAGTGTTGC+TGG + chr4.4:88179426-88179445 None:intergenic 35.0%
! GCTGAAGCATTCAATGTTAT+AGG - chr4.4:88180317-88180336 MS.gene034047:intron 35.0%
! GGTTATCTTTTCTATTCACC+TGG + chr4.4:88179272-88179291 None:intergenic 35.0%
! TCAGAGGTTATGTCTTTTTC+TGG + chr4.4:88179822-88179841 None:intergenic 35.0%
! TTGATGTCACTTAACATTGG+AGG - chr4.4:88179939-88179958 MS.gene034047:intron 35.0%
!! GATATGCATTTTTCAGTTGC+AGG + chr4.4:88179153-88179172 None:intergenic 35.0%
!! TGGAAATGATGGATGGATAT+CGG - chr4.4:88180478-88180497 MS.gene034047:intron 35.0%
!! TTCATTGCTGATTCTCATCT+TGG - chr4.4:88178205-88178224 MS.gene034047:CDS 35.0%
!!! GTGGTAATGCTTTGTTTTCT+AGG - chr4.4:88179766-88179785 MS.gene034047:intron 35.0%
AACAGGGAATGATACTCTTG+TGG - chr4.4:88179085-88179104 MS.gene034047:intron 40.0%
AACCCAAAACTTCGAAGTTC+CGG - chr4.4:88180004-88180023 MS.gene034047:intron 40.0%
ATTACACTAAGTGAACCAGC+CGG + chr4.4:88180026-88180045 None:intergenic 40.0%
CAGCAATTGTCGAGACTATA+AGG - chr4.4:88180195-88180214 MS.gene034047:intron 40.0%
CGAAGAAGAGCAATCAATGA+AGG - chr4.4:88180218-88180237 MS.gene034047:intron 40.0%
CTTAACATTGGAGGCTCATT+TGG - chr4.4:88179948-88179967 MS.gene034047:intron 40.0%
CTTATGTCCATTGGAAATGG+TGG - chr4.4:88179216-88179235 MS.gene034047:CDS 40.0%
GACATGTTCAACACTGAATG+AGG + chr4.4:88180257-88180276 None:intergenic 40.0%
GATCATCTTGGATGGAAACT+TGG - chr4.4:88179390-88179409 MS.gene034047:CDS 40.0%
GCTCTTATGTCCATTGGAAA+TGG - chr4.4:88179213-88179232 MS.gene034047:CDS 40.0%
GTAGTATGTAGTTGGGCTTA+TGG - chr4.4:88180578-88180597 MS.gene034047:CDS 40.0%
GTCTTTGCACTAATCCTAAC+CGG + chr4.4:88180128-88180147 None:intergenic 40.0%
GTGTGGAAGAAGAGATGAAT+AGG - chr4.4:88178324-88178343 MS.gene034047:CDS 40.0%
GTTTCCATCCAAGATGATCT+TGG + chr4.4:88179389-88179408 None:intergenic 40.0%
GTTTGGTACAAATGAGGGAA+GGG + chr4.4:88179015-88179034 None:intergenic 40.0%
TCAATTGTGCATAGACTCCA+AGG + chr4.4:88179861-88179880 None:intergenic 40.0%
TCCAATGGACATAAGAGCAA+GGG + chr4.4:88179211-88179230 None:intergenic 40.0%
TCTAGTGAAATCTCTCTCCT+CGG - chr4.4:88178702-88178721 MS.gene034047:CDS 40.0%
TGGTGGTTGCAACAGATAAA+AGG + chr4.4:88178162-88178181 None:intergenic 40.0%
TGTTGAACATGTCTGCAATG+TGG - chr4.4:88180264-88180283 MS.gene034047:intron 40.0%
TGTTTGGTACAAATGAGGGA+AGG + chr4.4:88179016-88179035 None:intergenic 40.0%
TTCCAATGGACATAAGAGCA+AGG + chr4.4:88179212-88179231 None:intergenic 40.0%
TTTGGTACAAATGAGGGAAG+GGG + chr4.4:88179014-88179033 None:intergenic 40.0%
! CGACATAGTCTTGGGAAATT+CGG + chr4.4:88180363-88180382 None:intergenic 40.0%
! CTTGGATGGAAACTTGGTTT+TGG - chr4.4:88179396-88179415 MS.gene034047:CDS 40.0%
! GGTGTTACATAGCTCATTTG+TGG - chr4.4:88178455-88178474 MS.gene034047:CDS 40.0%
! TTTTCAGTTGCAGGAATGCA+AGG + chr4.4:88179144-88179163 None:intergenic 40.0%
!! AGCATCGCTTCATCACTTTT+CGG - chr4.4:88180383-88180402 MS.gene034047:intron 40.0%
!! AGGTTTCTTGCTGTTGGTTT+AGG - chr4.4:88178229-88178248 MS.gene034047:CDS 40.0%
!! ATCACTTTTCGGACTAGGAA+TGG - chr4.4:88180394-88180413 MS.gene034047:intron 40.0%
!! GCAATGAAAGCACCAATAAG+AGG + chr4.4:88178193-88178212 None:intergenic 40.0%
!! GCCGATTCGACAAGTATTAT+TGG - chr4.4:88180516-88180535 MS.gene034047:intron 40.0%
!! GCTTTTTCCTCAACTTTGCT+TGG - chr4.4:88180286-88180305 MS.gene034047:intron 40.0%
!! GTATCGTGTTGGTGTCAATA+TGG - chr4.4:88178426-88178445 MS.gene034047:CDS 40.0%
!! TTGCTGATTCTCATCTTGGA+AGG - chr4.4:88178209-88178228 MS.gene034047:CDS 40.0%
!! TTGGTGTCAATATGGAGTGT+CGG - chr4.4:88178434-88178453 MS.gene034047:CDS 40.0%
AAGATGCTTGTAGACCACAG+TGG + chr4.4:88179921-88179940 None:intergenic 45.0%
AGAGATTTCACTAGAGGAGG+AGG + chr4.4:88178697-88178716 None:intergenic 45.0%
ATCGAATGTCCGTATCGTGT+TGG - chr4.4:88178415-88178434 MS.gene034047:CDS 45.0%
ATGGATCAGCTTCAGATCCT+TGG - chr4.4:88179841-88179860 MS.gene034047:intron 45.0%
ATTCCACCCAAGCAAAGTTG+AGG + chr4.4:88180296-88180315 None:intergenic 45.0%
CCAATGCCCTTTGGTTATCA+GGG + chr4.4:88179293-88179312 None:intergenic 45.0%
CCTGTTCTTATAAGCCGCAA+CGG + chr4.4:88179532-88179551 None:intergenic 45.0%
CTTCAATGCTCCATAGCGTT+TGG - chr4.4:88179240-88179259 MS.gene034047:CDS 45.0%
CTTGGCTCAACGATTTGAAG+GGG - chr4.4:88178546-88178565 MS.gene034047:CDS 45.0%
GACAAGATTAATGGAGGGGA+TGG - chr4.4:88178498-88178517 MS.gene034047:CDS 45.0%
GAGAGAGATTTCACTAGAGG+AGG + chr4.4:88178700-88178719 None:intergenic 45.0%
GAGGAGAGAGATTTCACTAG+AGG + chr4.4:88178703-88178722 None:intergenic 45.0%
GCAAAGACAAGAATGGGACT+TGG - chr4.4:88180140-88180159 MS.gene034047:intron 45.0%
GCTTGGCTCAACGATTTGAA+GGG - chr4.4:88178545-88178564 MS.gene034047:CDS 45.0%
TACATTCCAGCCGAGTTCTT+AGG + chr4.4:88179570-88179589 None:intergenic 45.0%
TCCAATGCCCTTTGGTTATC+AGG + chr4.4:88179294-88179313 None:intergenic 45.0%
TCCCTTGCTCTTATGTCCAT+TGG - chr4.4:88179207-88179226 MS.gene034047:CDS 45.0%
TCGACTACCACCTAAGAACT+CGG - chr4.4:88179557-88179576 MS.gene034047:CDS 45.0%
TCGTACAAAGCCGGTAAACA+AGG + chr4.4:88179505-88179524 None:intergenic 45.0%
TGCTTGGCTCAACGATTTGA+AGG - chr4.4:88178544-88178563 MS.gene034047:CDS 45.0%
TTGAAGGCCACCATTTCCAA+TGG + chr4.4:88179226-88179245 None:intergenic 45.0%
TTTCCTCAACTTTGCTTGGG+TGG - chr4.4:88180290-88180309 MS.gene034047:intron 45.0%
! AGCCGGAACTTCGAAGTTTT+GGG + chr4.4:88180009-88180028 None:intergenic 45.0%
! ATCTGAAGCTGATCCATCAG+AGG + chr4.4:88179838-88179857 None:intergenic 45.0%
! CAGTTGGTACTATGCTTCCT+CGG - chr4.4:88179323-88179342 MS.gene034047:CDS 45.0%
! CTTGGAAGGTTTCTTGCTGT+TGG - chr4.4:88178223-88178242 MS.gene034047:CDS 45.0%
! GGAGATGATATTGAGGTGCT+TGG + chr4.4:88178993-88179012 None:intergenic 45.0%
!! CAAACGCTATGGAGCATTGA+AGG + chr4.4:88179242-88179261 None:intergenic 45.0%
!! GCTTCATCACTTTTCGGACT+AGG - chr4.4:88180389-88180408 MS.gene034047:intron 45.0%
AACGGTCACTCGTACAAAGC+CGG + chr4.4:88179514-88179533 None:intergenic 50.0%
AAGGAGGAGGTCTTGCTTGT+GGG + chr4.4:88179125-88179144 None:intergenic 50.0%
ACCTCGAGAGGTGGAAATGA+TGG - chr4.4:88180467-88180486 MS.gene034047:intron 50.0%
AGCGATGCTCGACATAGTCT+TGG + chr4.4:88180372-88180391 None:intergenic 50.0%
AGCTCAGAGGAAAACCGGTT+AGG - chr4.4:88180111-88180130 MS.gene034047:intron 50.0%
AGGTCTTGCTTGTGGGATCA+TGG + chr4.4:88179118-88179137 None:intergenic 50.0%
ATTCCAGCCGAGTTCTTAGG+TGG + chr4.4:88179567-88179586 None:intergenic 50.0%
CAAAACTTCGAAGTTCCGGC+TGG - chr4.4:88180008-88180027 MS.gene034047:intron 50.0%
CCGTTGCGGCTTATAAGAAC+AGG - chr4.4:88179529-88179548 MS.gene034047:CDS 50.0%
CTCAACGATTTGAAGGGGCA+CGG - chr4.4:88178551-88178570 MS.gene034047:CDS 50.0%
CTCGAAGCTCAGAGGAAAAC+CGG - chr4.4:88180106-88180125 MS.gene034047:intron 50.0%
CTTTGTACGAGTGACCGTTG+CGG - chr4.4:88179515-88179534 MS.gene034047:CDS 50.0%
GATTTCACTAGAGGAGGAGG+AGG + chr4.4:88178694-88178713 None:intergenic 50.0%
GCGATGCTCGACATAGTCTT+GGG + chr4.4:88180371-88180390 None:intergenic 50.0%
GGGAAGGGGAGATGATATTG+AGG + chr4.4:88179000-88179019 None:intergenic 50.0%
TCAGTTGCAGGAATGCAAGG+AGG + chr4.4:88179141-88179160 None:intergenic 50.0%
TCCATCATTTCCACCTCTCG+AGG + chr4.4:88180471-88180490 None:intergenic 50.0%
TGGCTTATTGCCACAGTGAG+TGG - chr4.4:88180536-88180555 MS.gene034047:intron 50.0%
TTGTGAGATTTCGACGGGAG+AGG - chr4.4:88178592-88178611 MS.gene034047:CDS 50.0%
! CAGCCGGAACTTCGAAGTTT+TGG + chr4.4:88180010-88180029 None:intergenic 50.0%
! CCCTGATAACCAAAGGGCAT+TGG - chr4.4:88179290-88179309 MS.gene034047:CDS 50.0%
! CGAGAGGTGGAAATGATGGA+TGG - chr4.4:88180471-88180490 MS.gene034047:intron 50.0%
ATGCTCCATAGCGTTTGGCG+CGG - chr4.4:88179245-88179264 MS.gene034047:CDS 55.0%
CAAGGAGGAGGTCTTGCTTG+TGG + chr4.4:88179126-88179145 None:intergenic 55.0%
CTACCACCTAAGAACTCGGC+TGG - chr4.4:88179561-88179580 MS.gene034047:CDS 55.0%
GAAAGGGTAACCTCGAGAGG+TGG - chr4.4:88180458-88180477 MS.gene034047:intron 55.0%
GTCGAAAGGGTAACCTCGAG+AGG - chr4.4:88180455-88180474 MS.gene034047:intron 55.0%
GTTGCAGGAATGCAAGGAGG+AGG + chr4.4:88179138-88179157 None:intergenic 55.0%
! CGGTTTTCCTCTGAGCTTCG+AGG + chr4.4:88180108-88180127 None:intergenic 55.0%
! TTCGGACTAGGAATGGCCGT+AGG - chr4.4:88180401-88180420 MS.gene034047:intron 55.0%
CCTCTAGCCTCGAAGCTCAG+AGG - chr4.4:88180098-88180117 MS.gene034047:intron 60.0%
CCTCTGAGCTTCGAGGCTAG+AGG + chr4.4:88180101-88180120 None:intergenic 60.0%
CATAGCGTTTGGCGCGGACC+AGG - chr4.4:88179251-88179270 MS.gene034047:CDS 65.0%
CTGGTCCGCGCCAAACGCTA+TGG + chr4.4:88179253-88179272 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 88178115 88180697 88178115 ID=MS.gene034047
chr4.4 mRNA 88178115 88180697 88178115 ID=MS.gene034047.t1;Parent=MS.gene034047
chr4.4 exon 88180542 88180697 88180542 ID=MS.gene034047.t1.exon1;Parent=MS.gene034047.t1
chr4.4 CDS 88180542 88180697 88180542 ID=cds.MS.gene034047.t1;Parent=MS.gene034047.t1
chr4.4 exon 88179175 88179722 88179175 ID=MS.gene034047.t1.exon2;Parent=MS.gene034047.t1
chr4.4 CDS 88179175 88179722 88179175 ID=cds.MS.gene034047.t1;Parent=MS.gene034047.t1
chr4.4 exon 88178115 88179024 88178115 ID=MS.gene034047.t1.exon3;Parent=MS.gene034047.t1
chr4.4 CDS 88178115 88179024 88178115 ID=cds.MS.gene034047.t1;Parent=MS.gene034047.t1
Gene Sequence

>MS.gene034047

ATGGGGAGTTATAATCTTCATTTATCTAAAGCTACTCATATACTCCTTTTATCTGTTGCAACCACCAATTTCATGCCTCTTATTGGTGCTTTCATTGCTGATTCTCATCTTGGAAGGTTTCTTGCTGTTGGTTTAGGTTCTATCATCACTTTCATGGTAAGTAGACTACTTTTCATTTTTCCTATCATTATCATGTGAATGATTAACTTGTGTGGAAGAAGAGATGAATAGGATATGTATTTTGACTCATTTGCTAAAAAGCACCGACACAAACACTAGACATTTTAGAAAATGGAATTGATCGAATGTCCGTATCGTGTTGGTGTCAATATGGAGTGTCGGTGTTACATAGCTCATTTGTGGACCAATTGTTTCATTGTGAAGACAAGATTAATGGAGGGGATGGAAAGATTTTCAATGAATCTTTCTTGCTTGGCTCAACGATTTGAAGGGGCACGGCATCTATCCTCTCTAATATTGTGAGATTTCGACGGGAGAGGATACGTGTTTAAGATATTTTAAACATGTTGACTTTAATATAACATGAAAGTTTAGAGGTTGTAAAAAGTAATTAGTCCTCCTCCTCCTCTAGTGAAATCTCTCTCCTCGGTGAATCAAATAAAGATGATGCATTTAGGTGTGAAGAGCTTAACCAGATACTTATTTAATATTTGGCAATCTTAAAGAGCTTCTTTAATAACAGGTTATAGTAGAACTCTGCATAAGCTATGTATAAACTATTTTAGTAAGTTACTACTATGAGCTTATGAAATAAGCTTAAAATAGTTTATGAATACTTCATACGTTATGTTATAAACTCGTTCAAGCTCTTACATAAATGCTTACGTCAAAAGTTAAGCACAAAATAAGCTCTTCCAAGCACCTCAATATCATCTCCCCTTCCCTCATTTGTACCAAACAATGTAAATGATTTTGTATTGAAAAACAACTCAATTTTCTTCCTTTTGGTAACAGGGAATGATACTCTTGTGGCTAACAGCCATGATCCCACAAGCAAGACCTCCTCCTTGCATTCCTGCAACTGAAAAATGCATATCTGCAACAACTTCACAAATGGCAATGCTGATTTCTTCCCTTGCTCTTATGTCCATTGGAAATGGTGGCCTTCAATGCTCCATAGCGTTTGGCGCGGACCAGGTGAATAGAAAAGATAACCCTGATAACCAAAGGGCATTGGAAATATTCTTCAGTTGGTACTATGCTTCCTCGGCTATTTCTGTCATAATTGCATTCACGGGAATAGTATATATCCAAGATCATCTTGGATGGAAACTTGGTTTTGGTGTTCCAGCAACACTTATGTTCTTTTCCACTTTCTTCTTTTTTCTTGCTTCTCCACTTTATGTAAAGAATAAAACAAATAGCACCTTGTTTACCGGCTTTGTACGAGTGACCGTTGCGGCTTATAAGAACAGGAAAATTCGACTACCACCTAAGAACTCGGCTGGAATGTATCATCATATAAAGGATTCTGAACTTACTGTTCCAACTGATAAAATAAGGTTGAATCACTTGCATATTCACATTAGTATTAGAGTCCCTACATTTAGATAAGTTGATATTCAAATTATTGCCAATTTTTGTTGTCATTTTGTGTTAAAATATCATTATGTATTAATATACTGAATTTGTGGTAATGCTTTGTTTTCTAGGTTTCTGAATAAAGCTTGCTTCATTAGAAGTCCAGAAAAAGACATAACCTCTGATGGATCAGCTTCAGATCCTTGGAGTCTATGCACAATTGATCAAGTAGAAGAATTAAAAGCTATCATTAAAGTTATTCCACTGTGGTCTACAAGCATCTTGATGTCACTTAACATTGGAGGCTCATTTGGATTACTGCAAGCGAAATCGCTGAACAGACACATAACCCAAAACTTCGAAGTTCCGGCTGGTTCACTTAGTGTAATCATGATATTCACAATATTTATATGGATAGCTCTTTATGATCGTGTCGTTATTCCTCTAGCCTCGAAGCTCAGAGGAAAACCGGTTAGGATTAGTGCAAAGACAAGAATGGGACTTGGATTGTTTTTCTCTTTTCTTCACTTGGTAACTGCAGCAATTGTCGAGACTATAAGGCGAAGAAGAGCAATCAATGAAGGATATGGTAATGATCCTCATTCAGTGTTGAACATGTCTGCAATGTGGCTTTTTCCTCAACTTTGCTTGGGTGGAATAGCTGAAGCATTCAATGTTATAGGCCAAAATGAGTTCTACTATACCGAATTTCCCAAGACTATGTCGAGCATCGCTTCATCACTTTTCGGACTAGGAATGGCCGTAGGATACATTATATCTTCTTTTTTATTCAGCATTGTCGAAAGGGTAACCTCGAGAGGTGGAAATGATGGATGGATATCGGATAATATTAACAAGGGCCGATTCGACAAGTATTATTGGCTTATTGCCACAGTGAGTGGTCTTAATATACTGTATTATGTAGTATGTAGTTGGGCTTATGGACCTACTGCTGATGTAGAATCAAAAGTAAGTGAAGATGAGGAAGAATTGCCATTAATTGACTTCAGAAATGAGGGTTCATATGACAAGGATAACTGA
Protein sequence

>MS.gene034047.t1

MGSYNLHLSKATHILLLSVATTNFMPLIGAFIADSHLGRFLAVGLGSIITFMGMILLWLTAMIPQARPPPCIPATEKCISATTSQMAMLISSLALMSIGNGGLQCSIAFGADQVNRKDNPDNQRALEIFFSWYYASSAISVIIAFTGIVYIQDHLGWKLGFGVPATLMFFSTFFFFLASPLYVKNKTNSTLFTGFVRVTVAAYKNRKIRLPPKNSAGMYHHIKDSELTVPTDKIRFLNKACFIRSPEKDITSDGSASDPWSLCTIDQVEELKAIIKVIPLWSTSILMSLNIGGSFGLLQAKSLNRHITQNFEVPAGSLSVIMIFTIFIWIALYDRVVIPLASKLRGKPVRISAKTRMGLGLFFSFLHLVTAAIVETIRRRRAINEGYGNDPHSVLNMSAMWLFPQLCLGGIAEAFNVIGQNEFYYTEFPKTMSSIASSLFGLGMAVGYIISSFLFSIVERVTSRGGNDGWISDNINKGRFDKYYWLIATVSGLNILYYVVCSWAYGPTADVESKVSEDEEELPLIDFRNEGSYDKDN