AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21157

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21157.t1 MTR_5g012270 96.569 583 18 1 1 581 1 583 0.0 1157
MS.gene21157.t1 MTR_5g012290 76.207 580 136 2 1 579 1 579 0.0 907
MS.gene21157.t1 MTR_4g101380 70.962 582 166 2 1 579 1 582 0.0 837
MS.gene21157.t1 MTR_4g114340 52.014 571 248 7 12 573 9 562 0.0 540
MS.gene21157.t1 MTR_5g085850 48.214 560 265 6 15 570 18 556 1.10e-175 510
MS.gene21157.t1 MTR_4g015080 43.705 556 303 6 17 570 24 571 2.33e-156 460
MS.gene21157.t1 MTR_7g067010 43.110 566 308 8 7 570 8 561 5.87e-154 454
MS.gene21157.t1 MTR_3g462710 44.086 558 300 8 17 570 14 563 4.44e-151 446
MS.gene21157.t1 MTR_5g055000 41.652 557 318 5 25 578 16 568 1.74e-150 445
MS.gene21157.t1 MTR_7g098200 41.459 562 310 6 17 571 19 568 2.32e-146 436
MS.gene21157.t1 MTR_7g088825 38.869 566 333 5 23 581 18 577 1.15e-143 429
MS.gene21157.t1 MTR_3g112460 39.895 569 320 7 17 581 13 563 2.26e-143 427
MS.gene21157.t1 MTR_7g098200 41.459 562 300 8 17 571 19 558 2.55e-142 425
MS.gene21157.t1 MTR_3g112470 39.330 567 326 8 17 581 13 563 1.00e-138 415
MS.gene21157.t1 MTR_7g098040 39.649 570 323 7 7 570 18 572 2.83e-137 412
MS.gene21157.t1 MTR_7g098160 39.895 569 323 6 7 570 17 571 1.60e-136 410
MS.gene21157.t1 MTR_1g075440 38.394 573 334 6 8 570 3 566 1.39e-135 407
MS.gene21157.t1 MTR_7g098230 39.411 543 314 5 34 571 33 565 2.29e-135 407
MS.gene21157.t1 MTR_1g041835 39.931 581 325 8 7 577 11 577 1.05e-134 405
MS.gene21157.t1 MTR_3g112450 38.995 577 322 10 14 581 10 565 5.92e-134 402
MS.gene21157.t1 MTR_7g088790 39.455 550 321 4 27 570 29 572 1.29e-133 403
MS.gene21157.t1 MTR_7g098180 37.866 581 345 5 5 579 9 579 3.29e-133 402
MS.gene21157.t1 MTR_7g098090 39.724 579 315 8 7 570 17 576 1.48e-132 400
MS.gene21157.t1 MTR_7g098220 39.336 572 328 6 7 573 14 571 3.20e-132 399
MS.gene21157.t1 MTR_7g010820 40.248 564 317 8 17 569 16 570 2.62e-130 394
MS.gene21157.t1 MTR_1g041815 38.699 584 335 8 7 581 11 580 6.29e-130 393
MS.gene21157.t1 MTR_4g019580 37.433 561 333 8 15 571 24 570 3.97e-129 391
MS.gene21157.t1 MTR_5g014150 38.028 568 339 8 7 567 24 585 3.04e-128 389
MS.gene21157.t1 MTR_5g038060 38.584 565 326 7 25 577 29 584 4.68e-128 389
MS.gene21157.t1 MTR_4g098800 38.732 568 330 9 7 567 16 572 4.84e-128 388
MS.gene21157.t1 MTR_2g005130 38.103 580 327 11 6 578 19 573 5.05e-128 388
MS.gene21157.t1 MTR_7g065080 38.011 563 326 8 17 575 21 564 5.11e-128 388
MS.gene21157.t1 MTR_3g069500 39.384 584 320 12 6 580 13 571 2.69e-127 386
MS.gene21157.t1 MTR_4g098800 38.732 568 330 9 7 567 81 637 3.88e-127 388
MS.gene21157.t1 MTR_3g069420 36.778 571 342 11 6 570 12 569 4.71e-125 380
MS.gene21157.t1 MTR_1g042310 36.130 584 351 8 7 581 10 580 6.66e-125 380
MS.gene21157.t1 MTR_7g088820 34.991 563 359 5 17 576 17 575 1.06e-124 379
MS.gene21157.t1 MTR_3g072300 37.925 559 335 8 17 572 14 563 1.66e-123 376
MS.gene21157.t1 MTR_3g069440 38.908 568 326 9 17 577 23 576 3.52e-123 375
MS.gene21157.t1 MTR_7g098220 38.920 537 313 5 42 573 1 527 4.94e-123 374
MS.gene21157.t1 MTR_7g005930 38.061 557 320 10 19 567 27 566 5.20e-123 375
MS.gene21157.t1 MTR_3g462710 45.638 447 232 7 128 570 15 454 5.98e-123 370
MS.gene21157.t1 MTR_4g033325 37.478 579 329 7 4 578 8 557 2.26e-122 372
MS.gene21157.t1 MTR_7g013270 37.258 569 332 10 8 567 71 623 4.36e-122 374
MS.gene21157.t1 MTR_1g041875 35.889 574 343 7 17 581 19 576 6.97e-122 372
MS.gene21157.t1 MTR_3g100980 37.213 567 330 10 22 578 11 561 6.88e-121 369
MS.gene21157.t1 MTR_0294s0020 36.749 566 325 7 17 578 21 557 2.77e-120 367
MS.gene21157.t1 MTR_4g033315 36.870 575 332 6 8 578 12 559 4.93e-120 366
MS.gene21157.t1 MTR_4g033310 36.870 575 332 6 8 578 12 559 4.93e-120 366
MS.gene21157.t1 MTR_4g107670 37.805 574 323 10 17 571 14 572 1.66e-119 365
MS.gene21157.t1 MTR_1g116930 37.568 551 323 8 23 567 19 554 3.30e-119 365
MS.gene21157.t1 MTR_8g087810 37.479 587 348 10 8 579 2 584 4.10e-118 362
MS.gene21157.t1 MTR_8g038880 38.489 569 313 8 26 580 19 564 7.67e-118 361
MS.gene21157.t1 MTR_8g087780 36.986 584 354 8 8 579 2 583 3.09e-117 360
MS.gene21157.t1 MTR_2g017750 37.088 577 346 10 17 578 24 598 7.04e-117 360
MS.gene21157.t1 MTR_8g469480 36.528 553 330 7 30 570 41 584 9.21e-117 359
MS.gene21157.t1 MTR_1g042310 35.649 547 334 7 44 581 6 543 5.71e-116 356
MS.gene21157.t1 MTR_3g005010 37.011 562 308 8 17 570 20 543 1.64e-115 354
MS.gene21157.t1 MTR_8g469310 38.287 572 319 8 17 570 16 571 1.72e-115 355
MS.gene21157.t1 MTR_7g088820 35.225 511 326 4 69 576 5 513 3.42e-115 353
MS.gene21157.t1 MTR_3g100980 35.462 595 330 11 22 578 11 589 7.39e-115 354
MS.gene21157.t1 MTR_1g042310 35.675 541 330 7 50 581 5 536 9.92e-115 352
MS.gene21157.t1 MTR_4g107530 35.778 559 334 10 30 573 36 584 2.25e-114 353
MS.gene21157.t1 MTR_7g065080 38.462 494 281 8 86 575 3 477 3.74e-114 349
MS.gene21157.t1 MTR_7g098200 40.343 466 259 6 113 571 1 454 5.01e-114 348
MS.gene21157.t1 MTR_7g006540 35.211 568 340 9 26 576 62 618 1.06e-112 350
MS.gene21157.t1 MTR_5g038380 36.632 576 338 7 17 577 18 581 1.08e-112 348
MS.gene21157.t1 MTR_1g041885 36.417 508 303 6 50 553 5 496 2.99e-112 345
MS.gene21157.t1 MTR_4g033375 38.310 509 282 8 11 515 16 496 4.26e-111 341
MS.gene21157.t1 MTR_4g033330 37.251 553 316 7 4 552 8 533 1.17e-110 341
MS.gene21157.t1 MTR_3g014790 35.866 566 334 8 24 581 14 558 1.68e-109 339
MS.gene21157.t1 MTR_4g107580 32.881 590 380 9 1 580 1 584 2.99e-109 340
MS.gene21157.t1 MTR_4g107510 35.027 551 345 7 30 571 39 585 3.08e-109 340
MS.gene21157.t1 MTR_1g085090 36.136 559 321 14 25 573 17 549 5.62e-109 339
MS.gene21157.t1 MTR_7g088820 36.247 469 294 4 116 581 4 470 2.42e-108 333
MS.gene21157.t1 MTR_7g111260 36.283 565 326 13 25 580 17 556 2.03e-107 335
MS.gene21157.t1 MTR_8g469470 33.390 584 365 9 3 570 6 581 7.79e-106 330
MS.gene21157.t1 MTR_4g033395 37.328 509 291 6 11 517 16 498 1.32e-105 327
MS.gene21157.t1 MTR_7g098150 39.955 448 253 4 7 451 17 451 1.05e-104 324
MS.gene21157.t1 MTR_5g038190 32.639 576 371 7 4 570 1 568 8.71e-102 320
MS.gene21157.t1 MTR_4g123537 34.495 574 344 11 7 571 21 571 5.19e-101 319
MS.gene21157.t1 MTR_4g033325 36.943 471 264 7 112 578 18 459 8.50e-98 305
MS.gene21157.t1 MTR_2g101650 30.864 567 365 10 24 577 21 573 1.15e-97 309
MS.gene21157.t1 MTR_8g103233 34.301 551 337 9 24 564 15 550 1.35e-97 309
MS.gene21157.t1 MTR_4g033325 37.194 449 257 6 134 578 6 433 2.23e-95 298
MS.gene21157.t1 MTR_4g033385 35.524 487 280 8 96 578 4 460 1.71e-93 294
MS.gene21157.t1 MTR_2g101640 31.459 569 360 10 25 577 21 575 2.05e-93 300
MS.gene21157.t1 MTR_4g011280 31.598 557 342 11 29 567 34 569 2.95e-93 298
MS.gene21157.t1 MTR_2g101590 31.826 553 356 11 25 567 24 565 7.43e-92 295
MS.gene21157.t1 MTR_2g101610 31.769 554 356 12 24 567 25 566 1.86e-89 289
MS.gene21157.t1 MTR_7g064780 31.607 560 362 11 23 571 22 571 5.39e-89 286
MS.gene21157.t1 MTR_1g026740 30.952 546 359 11 29 567 19 553 7.50e-89 286
MS.gene21157.t1 MTR_4g107610 31.317 562 368 8 23 576 27 578 1.04e-85 278
MS.gene21157.t1 MTR_2g093180 32.796 558 335 11 26 571 20 549 8.07e-85 275
MS.gene21157.t1 MTR_2g093640 32.374 556 339 10 26 571 20 548 3.88e-84 273
MS.gene21157.t1 MTR_7g064800 30.973 565 374 9 14 568 12 570 7.61e-84 273
MS.gene21157.t1 MTR_1g026750 31.579 551 357 12 25 567 16 554 3.70e-83 271
MS.gene21157.t1 MTR_2g093160 31.949 554 344 9 26 571 20 548 7.55e-83 269
MS.gene21157.t1 MTR_4g024930 32.168 572 342 13 5 567 18 552 7.69e-83 270
MS.gene21157.t1 MTR_2g101560 31.889 577 354 16 24 580 25 582 1.99e-82 271
MS.gene21157.t1 MTR_1g026770 30.530 547 359 11 29 567 17 550 9.98e-82 267
MS.gene21157.t1 MTR_1g057460 31.139 562 330 15 24 567 42 564 2.13e-80 264
MS.gene21157.t1 MTR_1g009200 29.799 547 361 7 25 561 25 558 5.87e-78 258
MS.gene21157.t1 MTR_6g008690 32.246 552 316 13 43 572 23 538 3.69e-75 249
MS.gene21157.t1 MTR_7g098210 31.332 533 269 11 46 571 1 443 4.94e-75 246
MS.gene21157.t1 MTR_1g057460 30.885 531 311 14 55 567 1 493 5.49e-75 247
MS.gene21157.t1 MTR_0236s0050 32.392 531 303 16 55 567 1 493 2.36e-73 243
MS.gene21157.t1 MTR_7g051880 31.777 557 335 11 14 567 11 525 8.44e-71 237
MS.gene21157.t1 MTR_7g052600 32.504 563 335 13 8 567 5 525 3.02e-70 235
MS.gene21157.t1 MTR_7g051830 31.317 562 341 10 9 567 6 525 7.17e-66 224
MS.gene21157.t1 MTR_4g033310 37.895 285 168 2 296 578 218 495 1.73e-61 211
MS.gene21157.t1 MTR_4g033310 39.785 186 102 2 8 193 12 187 1.66e-32 131
MS.gene21157.t1 MTR_1g009170 26.866 402 235 9 153 549 9 356 9.64e-36 138
MS.gene21157.t1 MTR_3g065350 39.752 161 89 4 339 499 1 153 1.74e-25 103
MS.gene21157.t1 MTR_2g093650 26.702 191 83 4 358 548 45 178 1.52e-13 70.5
MS.gene21157.t1 MTR_4g033390 34.409 93 59 1 487 577 30 122 5.32e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21157.t1 AT1G12110 60.481 582 220 6 3 579 2 578 0.0 694
MS.gene21157.t1 AT2G26690 52.595 578 250 9 12 580 9 571 0.0 573
MS.gene21157.t1 AT2G26690 52.087 551 240 9 39 580 3 538 0.0 538
MS.gene21157.t1 AT2G26690 51.756 541 237 9 49 580 1 526 0.0 525
MS.gene21157.t1 AT3G21670 48.936 564 260 7 14 570 16 558 1.88e-178 517
MS.gene21157.t1 AT5G01180 43.806 557 302 6 17 570 15 563 1.74e-164 481
MS.gene21157.t1 AT5G01180 43.806 557 302 6 17 570 15 563 1.74e-164 481
MS.gene21157.t1 AT5G01180 43.806 557 302 6 17 570 15 563 1.74e-164 481
MS.gene21157.t1 AT3G54140 42.504 567 312 7 7 570 8 563 3.69e-158 464
MS.gene21157.t1 AT3G54140 42.504 567 312 7 7 570 8 563 3.69e-158 464
MS.gene21157.t1 AT2G02040 41.459 562 318 8 17 575 31 584 3.82e-145 432
MS.gene21157.t1 AT1G68570 41.812 574 322 7 7 574 5 572 2.35e-140 420
MS.gene21157.t1 AT1G68570 42.620 542 301 5 39 574 5 542 1.41e-137 412
MS.gene21157.t1 AT1G59740 38.328 574 335 6 14 581 28 588 1.33e-135 407
MS.gene21157.t1 AT5G46050 39.146 562 320 5 17 570 17 564 1.85e-135 407
MS.gene21157.t1 AT1G62200 39.820 555 315 8 17 565 42 583 4.15e-134 404
MS.gene21157.t1 AT1G62200 38.660 582 313 10 17 565 56 626 1.24e-130 396
MS.gene21157.t1 AT1G62200 38.660 582 313 10 17 565 42 612 1.36e-130 395
MS.gene21157.t1 AT5G46040 38.681 561 324 7 17 570 17 564 1.06e-129 392
MS.gene21157.t1 AT1G33440 38.927 578 318 10 2 567 13 567 2.21e-126 384
MS.gene21157.t1 AT2G02020 37.729 546 296 7 17 561 32 534 2.50e-124 377
MS.gene21157.t1 AT2G40460 39.341 577 328 8 7 580 8 565 7.17e-124 377
MS.gene21157.t1 AT4G21680 40.035 572 323 8 7 569 12 572 1.36e-123 377
MS.gene21157.t1 AT1G32450 36.713 572 348 8 17 581 27 591 5.23e-122 373
MS.gene21157.t1 AT3G53960 37.258 569 338 9 8 570 18 573 7.19e-122 372
MS.gene21157.t1 AT2G37900 35.478 575 342 9 3 570 14 566 4.54e-120 367
MS.gene21157.t1 AT3G54450 36.201 558 334 5 17 570 3 542 7.85e-120 366
MS.gene21157.t1 AT1G69870 37.050 556 330 8 25 570 49 594 9.83e-120 367
MS.gene21157.t1 AT1G72140 36.724 580 312 9 9 573 16 555 3.14e-115 354
MS.gene21157.t1 AT5G19640 36.542 561 324 11 28 580 60 596 1.28e-113 351
MS.gene21157.t1 AT5G13400 35.325 569 344 8 23 577 60 618 3.29e-113 351
MS.gene21157.t1 AT1G22540 39.750 561 303 9 17 570 20 552 1.38e-109 339
MS.gene21157.t1 AT1G27040 37.702 557 330 7 17 569 13 556 2.54e-109 339
MS.gene21157.t1 AT1G27040 37.702 557 330 7 17 569 17 560 4.09e-109 338
MS.gene21157.t1 AT1G22570 37.193 570 329 9 9 570 12 560 1.79e-108 337
MS.gene21157.t1 AT1G69850 37.024 578 329 11 17 571 15 580 2.67e-108 337
MS.gene21157.t1 AT5G46050 39.440 464 259 5 115 570 3 452 2.78e-108 333
MS.gene21157.t1 AT2G37900 35.882 510 298 9 74 576 6 493 1.80e-107 332
MS.gene21157.t1 AT1G22550 35.802 567 334 8 12 570 15 559 2.52e-107 333
MS.gene21157.t1 AT1G27040 37.899 533 314 7 41 569 9 528 7.17e-105 326
MS.gene21157.t1 AT1G72120 34.974 569 338 7 8 570 10 552 1.57e-104 326
MS.gene21157.t1 AT1G72125 33.854 576 347 8 8 574 10 560 3.07e-104 325
MS.gene21157.t1 AT2G02020 34.799 546 291 8 17 561 32 513 5.00e-104 324
MS.gene21157.t1 AT1G72130 36.767 563 300 12 19 570 16 533 1.26e-101 318
MS.gene21157.t1 AT5G62730 34.386 570 334 11 17 573 46 588 1.49e-99 314
MS.gene21157.t1 AT1G18880 32.857 560 360 7 30 579 22 575 2.10e-99 314
MS.gene21157.t1 AT3G25260 35.081 553 309 10 17 567 11 515 5.59e-99 310
MS.gene21157.t1 AT3G47960 33.925 563 352 10 21 570 53 608 2.43e-98 312
MS.gene21157.t1 AT5G62680 32.550 553 355 8 30 570 46 592 2.78e-98 312
MS.gene21157.t1 AT1G22570 36.599 541 314 9 38 570 4 523 4.23e-97 306
MS.gene21157.t1 AT5G62730 34.296 554 324 11 33 573 3 529 5.84e-96 303
MS.gene21157.t1 AT1G27080 34.513 565 337 11 25 577 12 555 1.56e-95 303
MS.gene21157.t1 AT3G25280 34.539 553 318 13 17 567 11 521 4.24e-92 293
MS.gene21157.t1 AT1G52190 30.404 569 375 10 13 567 5 566 5.11e-91 293
MS.gene21157.t1 AT3G16180 31.818 572 362 13 13 567 5 565 1.55e-90 291
MS.gene21157.t1 AT1G27080 34.398 532 316 11 58 577 3 513 1.07e-84 274
MS.gene21157.t1 AT3G45710 29.338 559 359 10 22 573 18 547 4.49e-84 273
MS.gene21157.t1 AT3G45660 30.634 568 364 11 14 577 8 549 5.77e-84 273
MS.gene21157.t1 AT3G45660 30.634 568 364 11 14 577 8 549 5.77e-84 273
MS.gene21157.t1 AT3G45660 30.634 568 364 11 14 577 29 570 1.20e-83 272
MS.gene21157.t1 AT5G14940 31.250 544 327 9 43 567 23 538 9.41e-81 264
MS.gene21157.t1 AT3G45650 29.550 555 361 9 23 573 18 546 1.21e-80 264
MS.gene21157.t1 AT3G45680 30.179 560 354 11 26 577 19 549 6.33e-80 262
MS.gene21157.t1 AT1G72130 36.758 438 237 8 139 569 10 414 6.92e-80 258
MS.gene21157.t1 AT1G72130 36.758 438 237 8 139 569 10 414 6.92e-80 258
MS.gene21157.t1 AT3G45700 28.571 553 358 9 26 573 15 535 7.71e-77 253
MS.gene21157.t1 AT5G28470 28.444 559 376 10 25 573 15 559 4.12e-76 252
MS.gene21157.t1 AT3G45720 28.909 550 357 10 27 570 19 540 6.29e-76 251
MS.gene21157.t1 AT3G45650 28.227 581 361 10 23 573 18 572 9.83e-76 251
MS.gene21157.t1 AT3G01350 31.107 569 343 12 24 567 4 548 1.82e-74 248
MS.gene21157.t1 AT5G28470 28.623 559 373 12 25 573 15 557 2.41e-74 247
MS.gene21157.t1 AT1G69860 28.546 557 368 8 24 571 15 550 1.34e-72 243
MS.gene21157.t1 AT3G45690 29.242 554 314 16 26 571 17 500 6.46e-67 226
MS.gene21157.t1 AT5G11570 26.443 537 306 10 37 567 23 476 7.39e-49 177
MS.gene21157.t1 AT2G38100 29.245 530 323 17 55 573 2 490 5.05e-46 169
MS.gene21157.t1 AT2G38100 29.245 530 323 17 55 573 18 506 5.83e-46 170
MS.gene21157.t1 AT2G38100 29.245 530 323 17 55 573 24 512 7.62e-46 169

Find 143 sgRNAs with CRISPR-Local

Find 201 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTTGTTCGGTGGCTTTCT+AGG 0.219772 5.1:-5566566 MS.gene21157:CDS
TCGATGACATCCTTCTTAAT+TGG 0.224118 5.1:-5564928 MS.gene21157:CDS
TTATGATTATTACTGGCTTT+TGG 0.247074 5.1:-5564443 MS.gene21157:CDS
ATCAACCAGAAAACACTTAA+TGG 0.249004 5.1:+5564691 None:intergenic
AGGAGAGTCCTTTATGTATA+TGG 0.250618 5.1:-5564644 MS.gene21157:CDS
ATTCTTGAGCTCAAGATCTT+TGG 0.255372 5.1:-5564560 MS.gene21157:CDS
CTAATGACACGGTCATAAAA+AGG 0.258608 5.1:+5564886 None:intergenic
GGAGGCTTTATGATTATTAC+TGG 0.269804 5.1:-5564450 MS.gene21157:CDS
ACTTCAGCTTCCATGATATT+AGG 0.285256 5.1:-5566812 MS.gene21157:intron
TTTAACAGGTACAATGCATT+TGG 0.293906 5.1:-5566645 MS.gene21157:CDS
ACTAGGCATAACCGTGAATT+TGG 0.307350 5.1:-5566675 MS.gene21157:CDS
AATTTGGTCACATATTTAAC+AGG 0.308170 5.1:-5566659 MS.gene21157:CDS
AGTTCTTTCAATCTTATCTA+TGG 0.319020 5.1:-5564794 MS.gene21157:CDS
TTCTTGAGCTCAAGATCTTT+GGG 0.322864 5.1:-5564559 MS.gene21157:CDS
AAATCCAGTGTCCCTGGCTT+TGG 0.323797 5.1:-5565727 MS.gene21157:CDS
GGGCATCAACCATCGTGTTT+TGG 0.330504 5.1:-5565041 MS.gene21157:CDS
TCATTTATGCTTTGTTTGTT+CGG 0.333514 5.1:-5566578 MS.gene21157:CDS
CAGGGTAGAGATTGGGGTTA+TGG 0.343473 5.1:-5565574 MS.gene21157:CDS
CACGATGGTTGATGCCCATA+TGG 0.348505 5.1:+5565047 None:intergenic
CACTTCTTCTACATCGGTTA+AGG 0.362617 5.1:+5565092 None:intergenic
CGATGTAGAAGAAGTGAAAT+TGG 0.367647 5.1:-5565085 MS.gene21157:CDS
ATATGGTTAAATTTCTCAAT+TGG 0.370598 5.1:-5565663 MS.gene21157:CDS
AACACTTTCATAGTCCTTGA+TGG 0.371357 5.1:+5565152 None:intergenic
TCTGCAATCCATGGTTTACT+TGG 0.374332 5.1:+5564487 None:intergenic
CAACTCCAGTATGCTGTAAA+GGG 0.380330 5.1:+5564821 None:intergenic
ATTTGCACAATCACATGGTT+TGG 0.380470 5.1:-5564737 MS.gene21157:CDS
GGTATCACAGGCAACAACTA+TGG 0.391203 5.1:-5564989 MS.gene21157:CDS
CCCTGATGCCTATGACTGTA+AGG 0.397338 5.1:-5566870 MS.gene21157:CDS
TTAAGCACTTTCCTTGCAAC+TGG 0.398521 5.1:+5564862 None:intergenic
TGTTTACATACAAGATCATC+AGG 0.401362 5.1:-5565593 MS.gene21157:CDS
CTTACCCCTTTACAGCATAC+TGG 0.401457 5.1:-5564826 MS.gene21157:CDS
TGCTAAGAATGCTCCCCATA+TGG 0.401978 5.1:-5565062 MS.gene21157:CDS
AGCAAACAATACACAGTTAA+CGG 0.404699 5.1:-5565797 MS.gene21157:CDS
CGTAAACCTAGGATCTCTTA+TGG 0.404734 5.1:-5565629 MS.gene21157:CDS
TACCGCACAGCAAACAGTTT+CGG 0.411818 5.1:-5565333 MS.gene21157:intron
AGATCATCAGGGTAGAGATT+GGG 0.416744 5.1:-5565581 MS.gene21157:CDS
TCCACAGTGAATAAAATAAC+AGG 0.418647 5.1:-5564511 MS.gene21157:CDS
TATGTCACTGCACTTGGCAC+AGG 0.420682 5.1:-5565757 MS.gene21157:CDS
CCAACTCCAGTATGCTGTAA+AGG 0.422699 5.1:+5564820 None:intergenic
TATAAATTTGATGATATACT+TGG 0.422701 5.1:-5564407 MS.gene21157:CDS
TCAACCAGAAAACACTTAAT+GGG 0.427591 5.1:+5564692 None:intergenic
TGGAAAACCCTTACAGTCAT+AGG 0.432523 5.1:+5566862 None:intergenic
CCTTGCATGTTGGAGGACTT+AGG 0.434447 5.1:+5565839 None:intergenic
CCCTTACAGTCATAGGCATC+AGG 0.435507 5.1:+5566869 None:intergenic
TTAACAGGTACAATGCATTT+GGG 0.437090 5.1:-5566644 MS.gene21157:CDS
CCAGCAGAAAGATCTAAAAC+TGG 0.445798 5.1:-5566842 MS.gene21157:CDS
AAGGATGTCATCGATGCTGC+TGG 0.454794 5.1:+5564937 None:intergenic
AAGATCATCAGGGTAGAGAT+TGG 0.456867 5.1:-5565582 MS.gene21157:CDS
CAATTTCACTTCTTCTACAT+CGG 0.458029 5.1:+5565086 None:intergenic
AACCTCTGCTTCTTGATCCT+CGG 0.458174 5.1:+5565355 None:intergenic
CATGTTGGAGGACTTAGGCT+TGG 0.462061 5.1:+5565844 None:intergenic
GCGCTTTATGTCACTGCACT+TGG 0.467474 5.1:-5565763 MS.gene21157:CDS
AACAAAATTTGTAACTATGT+TGG 0.470158 5.1:+5566607 None:intergenic
TAAATATGTGACCAAATTCA+CGG 0.470251 5.1:+5566664 None:intergenic
TCGTGTTTGTGGCCGCTTGG+AGG 0.471555 5.1:-5565447 MS.gene21157:CDS
ATTCCTTACTGTTGCTTGAA+CGG 0.473367 5.1:+5566303 None:intergenic
CCTTTACAGCATACTGGAGT+TGG 0.477525 5.1:-5564820 MS.gene21157:CDS
TAATTGTCCCATATACATAA+AGG 0.480422 5.1:+5564636 None:intergenic
GAGAATAGTTCCAATTAAGA+AGG 0.481152 5.1:+5564918 None:intergenic
GGAGAGTCCTTTATGTATAT+GGG 0.481210 5.1:-5564643 MS.gene21157:CDS
GTATGTTCCTTGCATGTTGG+AGG 0.481231 5.1:+5565832 None:intergenic
TTTATCAGGAGGAGAAGTGA+TGG 0.484364 5.1:-5566711 MS.gene21157:intron
ATGTCACTGCACTTGGCACA+GGG 0.486739 5.1:-5565756 MS.gene21157:CDS
AATTGATCTGAACCAAAGCC+AGG 0.490669 5.1:+5565715 None:intergenic
TTGTGGCCGCTTGGAGGAAG+AGG 0.491260 5.1:-5565441 MS.gene21157:CDS
TCAGATTGCGGTCGTGTTTG+TGG 0.492490 5.1:-5565458 MS.gene21157:CDS
ACGACCGCAATCTGAGTCAA+TGG 0.494754 5.1:+5565466 None:intergenic
TAAAGAAGAGAAGAAACATA+TGG 0.498104 5.1:-5565680 MS.gene21157:CDS
CGGTCGTGTTTGTGGCCGCT+TGG 0.498598 5.1:-5565450 MS.gene21157:CDS
TGAACCAAAGCCAGGGACAC+TGG 0.499870 5.1:+5565723 None:intergenic
ACGATGGTTGATGCCCATAT+GGG 0.500071 5.1:+5565048 None:intergenic
TAAGTATCGGTACAAGAAAC+CGG 0.503825 5.1:-5565497 MS.gene21157:CDS
GTCACTGCACTTGGCACAGG+GGG 0.504363 5.1:-5565754 MS.gene21157:CDS
TAGGCGACACTTACATTGGA+AGG 0.509164 5.1:-5566547 MS.gene21157:intron
CTTAATGGGATCTTTGCCGT+TGG 0.512854 5.1:+5564706 None:intergenic
TTTGGACAAAGCGGCCATCA+AGG 0.514200 5.1:-5565166 MS.gene21157:CDS
AAGGACTATGAAAGTGTTGG+TGG 0.519137 5.1:-5565147 MS.gene21157:CDS
GGTTTAAAATCCAGTGTCCC+TGG 0.528879 5.1:-5565733 MS.gene21157:CDS
TTGTGAGAGACCTAATATCA+TGG 0.530965 5.1:+5566802 None:intergenic
CAGGAGGAGAAGTGATGGAG+AGG 0.532787 5.1:-5566706 MS.gene21157:CDS
GAAGGTTGCTGAGGAAGGCA+TGG 0.535846 5.1:-5564353 MS.gene21157:CDS
CCTCGGTGGTTCATGTAAGA+TGG 0.536385 5.1:+5565372 None:intergenic
CTGCAAAGACAGCGATATTG+AGG 0.538484 5.1:+5566326 None:intergenic
GATGACACTCTCGGTATCAC+AGG 0.543941 5.1:-5565001 MS.gene21157:CDS
ATTTGTAACTATGTTGGCAG+AGG 0.544121 5.1:+5566613 None:intergenic
TTAAGATTATCTGCAATCCA+TGG 0.549313 5.1:+5564478 None:intergenic
TATGTGGAACATGTAAGTAT+CGG 0.552487 5.1:-5565510 MS.gene21157:CDS
AATAATCATAAAGCCTCCCT+TGG 0.555546 5.1:+5564455 None:intergenic
GCTAAGAATGCTCCCCATAT+GGG 0.555683 5.1:-5565061 MS.gene21157:CDS
TTCTCAGAGAATGTCCAGAT+GGG 0.558361 5.1:-5564606 MS.gene21157:CDS
TTTCTCAGAGAATGTCCAGA+TGG 0.560013 5.1:-5564607 MS.gene21157:CDS
GTATGTGTACAAGGATAAGA+AGG 0.564955 5.1:-5564371 MS.gene21157:CDS
AAAGATCTAAAACTGGCGGT+TGG 0.565390 5.1:-5566835 MS.gene21157:CDS
TTTATGCTTTGTTTGTTCGG+TGG 0.566441 5.1:-5566575 MS.gene21157:CDS
TGGGATGAAAACTATGAGCA+TGG 0.566517 5.1:-5564587 MS.gene21157:CDS
GATCATCAGGGTAGAGATTG+GGG 0.567129 5.1:-5565580 MS.gene21157:CDS
AAGAGCAACGCGGAATCAGA+AGG 0.573794 5.1:+5565406 None:intergenic
ACAACTATGGATCGCCACAT+TGG 0.574441 5.1:-5564976 MS.gene21157:CDS
CAGATAATCTTAACCAAGGG+AGG 0.574651 5.1:-5564468 MS.gene21157:CDS
GTTGTATGTTCCTTGCATGT+TGG 0.576661 5.1:+5565829 None:intergenic
TCACTGCCATAAGAGATCCT+AGG 0.584138 5.1:+5565623 None:intergenic
TGCAAAGTGGTATGTGTACA+AGG 0.585233 5.1:-5564380 MS.gene21157:CDS
CCTGATGCCTATGACTGTAA+GGG 0.586443 5.1:-5566869 MS.gene21157:CDS
GAATCATGAAGATAAGTAAG+TGG 0.587811 5.1:-5565125 MS.gene21157:CDS
TTTCTAGGCGACACTTACAT+TGG 0.589042 5.1:-5566551 MS.gene21157:CDS
TATTACTTCATCGTAAACCT+AGG 0.589827 5.1:-5565640 MS.gene21157:CDS
TGAGTCAATGGACTACCTAC+CGG 0.592200 5.1:+5565478 None:intergenic
CCTAAGTCCTCCAACATGCA+AGG 0.595704 5.1:-5565839 MS.gene21157:CDS
GATAAGAAGGTTGCTGAGGA+AGG 0.597576 5.1:-5564358 MS.gene21157:CDS
CTCTGCTTCTTGATCCTCGG+TGG 0.598843 5.1:+5565358 None:intergenic
AGGATGTCATCGATGCTGCT+GGG 0.602143 5.1:+5564938 None:intergenic
TTAACATTTGCACAATCACA+TGG 0.609665 5.1:-5564742 MS.gene21157:CDS
GGGATGAAAACTATGAGCAT+GGG 0.611383 5.1:-5564586 MS.gene21157:CDS
ATCAAGGACTATGAAAGTGT+TGG 0.611419 5.1:-5565150 MS.gene21157:CDS
AGTTCAAATGATGACACTCT+CGG 0.615015 5.1:-5565010 MS.gene21157:CDS
AAGGTTGCTGAGGAAGGCAT+GGG 0.615646 5.1:-5564352 MS.gene21157:CDS
AGGATCTCTTATGGCAGTGA+CGG 0.616609 5.1:-5565620 MS.gene21157:CDS
CAAGGATAAGAAGGTTGCTG+AGG 0.616944 5.1:-5564362 MS.gene21157:CDS
GATGATCTTGTATGTAAACA+AGG 0.619329 5.1:+5565596 None:intergenic
ATGGAGAGGTTGACAACACT+AGG 0.626970 5.1:-5566692 MS.gene21157:CDS
TTGCAGATAATCTTAACCAA+GGG 0.627914 5.1:-5564471 MS.gene21157:CDS
CGATGGTTGATGCCCATATG+GGG 0.629413 5.1:+5565049 None:intergenic
TGGTTTGGTGAATGATCCAA+CGG 0.633408 5.1:-5564722 MS.gene21157:CDS
ATTGCAGATAATCTTAACCA+AGG 0.634000 5.1:-5564472 MS.gene21157:CDS
CACCGAGGATCAAGAAGCAG+AGG 0.635285 5.1:-5565357 MS.gene21157:CDS
GTGTCATTAGTCCAGTTGCA+AGG 0.637046 5.1:-5564873 MS.gene21157:CDS
CCTTACAGTCATAGGCATCA+GGG 0.637502 5.1:+5566870 None:intergenic
ATTGATCTGAACCAAAGCCA+GGG 0.638982 5.1:+5565716 None:intergenic
TAACAGGTCCAAGTAAACCA+TGG 0.640549 5.1:-5564495 MS.gene21157:CDS
TGTAAAGGGGTAAGCCCTTG+TGG 0.646381 5.1:+5564835 None:intergenic
AACTCCAGTATGCTGTAAAG+GGG 0.649597 5.1:+5564822 None:intergenic
TAGTCCATTGACTCAGATTG+CGG 0.654481 5.1:-5565470 MS.gene21157:CDS
TATCGGTACAAGAAACCGGT+AGG 0.659573 5.1:-5565493 MS.gene21157:CDS
TACCGAAACTGTTTGCTGTG+CGG 0.669157 5.1:+5565331 None:intergenic
TTGCAACTGGACTAATGACA+CGG 0.673194 5.1:+5564875 None:intergenic
GCAGAAAGATCTAAAACTGG+CGG 0.673481 5.1:-5566839 MS.gene21157:CDS
TGTCACTGCACTTGGCACAG+GGG 0.685312 5.1:-5565755 MS.gene21157:CDS
GTTTACATACAAGATCATCA+GGG 0.688578 5.1:-5565592 MS.gene21157:CDS
CAAGTGCCTCTTCCTCCAAG+CGG 0.708297 5.1:+5565435 None:intergenic
TATACTTGGCTTGTGCAAAG+TGG 0.710999 5.1:-5564393 MS.gene21157:CDS
TTGAACAGTCCAAAACACGA+TGG 0.740184 5.1:+5565032 None:intergenic
CCATCTTACATGAACCACCG+AGG 0.777518 5.1:-5565372 MS.gene21157:CDS
ATCGTCGTTAAAGAGCAACG+CGG 0.846283 5.1:+5565396 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTATTCATAATTTAAT+AGG - chr5.1:5565351-5565370 MS.gene21157:CDS 10.0%
!!! ATTATGATGAATTATTTTAT+AGG + chr5.1:5565252-5565271 None:intergenic 10.0%
!!! GTTTTATTCATAATTTAATA+GGG - chr5.1:5565352-5565371 MS.gene21157:CDS 10.0%
!! TAAAATCAATACAAAAGAAA+CGG + chr5.1:5564507-5564526 None:intergenic 15.0%
!!! AGATATATTTTATAAAGTGT+CGG - chr5.1:5565979-5565998 MS.gene21157:intron 15.0%
!!! GAAATGTTGATTATTAAATT+GGG + chr5.1:5565025-5565044 None:intergenic 15.0%
!!! TATAAATTTGATGATATACT+TGG - chr5.1:5566843-5566862 MS.gene21157:CDS 15.0%
!!! TATAGGAAAATTTGTTTTAT+AGG + chr5.1:5565235-5565254 None:intergenic 15.0%
!!! TGAAATGTTGATTATTAAAT+TGG + chr5.1:5565026-5565045 None:intergenic 15.0%
!! AACAAAATTTGTAACTATGT+TGG + chr5.1:5564646-5564665 None:intergenic 20.0%
!! AATAAATTTATAGCAAGTTG+CGG + chr5.1:5565100-5565119 None:intergenic 20.0%
!! ATACAAAAGACAAAATCAAA+GGG + chr5.1:5565167-5565186 None:intergenic 20.0%
!! ATATGGTTAAATTTCTCAAT+TGG - chr5.1:5565587-5565606 MS.gene21157:CDS 20.0%
!! TATCTATAACACTATCTAAA+GGG - chr5.1:5565184-5565203 MS.gene21157:intron 20.0%
!!! AACATAGTTACAAATTTTGT+TGG - chr5.1:5564645-5564664 MS.gene21157:CDS 20.0%
! AATATCTACGATAAGATACA+AGG + chr5.1:5565956-5565975 None:intergenic 25.0%
! AATTTGGTCACATATTTAAC+AGG - chr5.1:5564591-5564610 MS.gene21157:CDS 25.0%
! AGTTCTTTCAATCTTATCTA+TGG - chr5.1:5566456-5566475 MS.gene21157:intron 25.0%
! ATACTTGTTCGATATTAGTA+AGG - chr5.1:5565129-5565148 MS.gene21157:CDS 25.0%
! ATATCTACGATAAGATACAA+GGG + chr5.1:5565955-5565974 None:intergenic 25.0%
! GAAATTTGATTGATACTGTT+AGG + chr5.1:5564844-5564863 None:intergenic 25.0%
! GATACAAAAGACAAAATCAA+AGG + chr5.1:5565168-5565187 None:intergenic 25.0%
! GTATCTATAACACTATCTAA+AGG - chr5.1:5565183-5565202 MS.gene21157:intron 25.0%
! TAAAGAAGAGAAGAAACATA+TGG - chr5.1:5565570-5565589 MS.gene21157:CDS 25.0%
! TAAATATGTGACCAAATTCA+CGG + chr5.1:5564589-5564608 None:intergenic 25.0%
! TAATTGTCCCATATACATAA+AGG + chr5.1:5566617-5566636 None:intergenic 25.0%
! TACAAAAGACAAAATCAAAG+GGG + chr5.1:5565166-5565185 None:intergenic 25.0%
! TACACTACAGTAAAAAAAAG+CGG - chr5.1:5565273-5565292 MS.gene21157:intron 25.0%
!! TAAAGGGAATATGAGATTTT+TGG - chr5.1:5565200-5565219 MS.gene21157:intron 25.0%
!! TATATTTTCGAACAGCTTTT+TGG - chr5.1:5566066-5566085 MS.gene21157:intron 25.0%
!!! TATGTGTCTTTTTGTTTATC+AGG - chr5.1:5564525-5564544 MS.gene21157:CDS 25.0%
!!! TCATTTATGCTTTGTTTGTT+CGG - chr5.1:5564672-5564691 MS.gene21157:CDS 25.0%
!!! TTATGATTATTACTGGCTTT+TGG - chr5.1:5566807-5566826 MS.gene21157:intron 25.0%
!!! TTTGAAATGATTTTCCAATG+TGG + chr5.1:5566291-5566310 None:intergenic 25.0%
AAGTGCTTAAAAATCCACAA+GGG - chr5.1:5566401-5566420 MS.gene21157:intron 30.0%
AGCAAACAATACACAGTTAA+CGG - chr5.1:5565453-5565472 MS.gene21157:CDS 30.0%
ATACTGTTAGGACTAATTGA+GGG + chr5.1:5564832-5564851 None:intergenic 30.0%
ATCAACCAGAAAACACTTAA+TGG + chr5.1:5566562-5566581 None:intergenic 30.0%
ATTGCAGATAATCTTAACCA+AGG - chr5.1:5566778-5566797 MS.gene21157:intron 30.0%
CAATTTCACTTCTTCTACAT+CGG + chr5.1:5566167-5566186 None:intergenic 30.0%
GAATCATGAAGATAAGTAAG+TGG - chr5.1:5566125-5566144 MS.gene21157:intron 30.0%
GAGAATAGTTCCAATTAAGA+AGG + chr5.1:5566335-5566354 None:intergenic 30.0%
GATGATCTTGTATGTAAACA+AGG + chr5.1:5565657-5565676 None:intergenic 30.0%
GTTTACATACAAGATCATCA+GGG - chr5.1:5565658-5565677 MS.gene21157:CDS 30.0%
TAATGACACGGTCATAAAAA+GGG + chr5.1:5566366-5566385 None:intergenic 30.0%
TATGTGGAACATGTAAGTAT+CGG - chr5.1:5565740-5565759 MS.gene21157:CDS 30.0%
TATTACTTCATCGTAAACCT+AGG - chr5.1:5565610-5565629 MS.gene21157:CDS 30.0%
TCAACCAGAAAACACTTAAT+GGG + chr5.1:5566561-5566580 None:intergenic 30.0%
TCCACAGTGAATAAAATAAC+AGG - chr5.1:5566739-5566758 MS.gene21157:intron 30.0%
TGTTTACATACAAGATCATC+AGG - chr5.1:5565657-5565676 MS.gene21157:CDS 30.0%
TTAACAGGTACAATGCATTT+GGG - chr5.1:5564606-5564625 MS.gene21157:CDS 30.0%
TTAACATTTGCACAATCACA+TGG - chr5.1:5566508-5566527 MS.gene21157:intron 30.0%
TTAAGATTATCTGCAATCCA+TGG + chr5.1:5566775-5566794 None:intergenic 30.0%
TTGCAGATAATCTTAACCAA+GGG - chr5.1:5566779-5566798 MS.gene21157:intron 30.0%
TTTAACAGGTACAATGCATT+TGG - chr5.1:5564605-5564624 MS.gene21157:CDS 30.0%
! AAAGTGCTTAAAAATCCACA+AGG - chr5.1:5566400-5566419 MS.gene21157:intron 30.0%
! ACAATTTTGTCTTGTGTTGT+GGG + chr5.1:5564360-5564379 None:intergenic 30.0%
! AGTGTCGGAATACTATAATA+GGG - chr5.1:5565994-5566013 MS.gene21157:intron 30.0%
!! AAGTGTCGGAATACTATAAT+AGG - chr5.1:5565993-5566012 MS.gene21157:intron 30.0%
!!! ACCTGTTATTTTATTCACTG+TGG + chr5.1:5566743-5566762 None:intergenic 30.0%
AACACTTTCATAGTCCTTGA+TGG + chr5.1:5566101-5566120 None:intergenic 35.0%
AATAATCATAAAGCCTCCCT+TGG + chr5.1:5566798-5566817 None:intergenic 35.0%
ACTTCAGCTTCCATGATATT+AGG - chr5.1:5564438-5564457 MS.gene21157:CDS 35.0%
AGGAGAGTCCTTTATGTATA+TGG - chr5.1:5566606-5566625 MS.gene21157:CDS 35.0%
AGTTCAAATGATGACACTCT+CGG - chr5.1:5566240-5566259 MS.gene21157:intron 35.0%
ATTCTTGAGCTCAAGATCTT+TGG - chr5.1:5566690-5566709 MS.gene21157:CDS 35.0%
ATTTGCACAATCACATGGTT+TGG - chr5.1:5566513-5566532 MS.gene21157:intron 35.0%
ATTTGTAACTATGTTGGCAG+AGG + chr5.1:5564640-5564659 None:intergenic 35.0%
CATAGTATGCTTTATCCATC+AGG - chr5.1:5565070-5565089 MS.gene21157:CDS 35.0%
CGATGTAGAAGAAGTGAAAT+TGG - chr5.1:5566165-5566184 MS.gene21157:intron 35.0%
CTAATGACACGGTCATAAAA+AGG + chr5.1:5566367-5566386 None:intergenic 35.0%
GATACTGTTAGGACTAATTG+AGG + chr5.1:5564833-5564852 None:intergenic 35.0%
GGAGAGTCCTTTATGTATAT+GGG - chr5.1:5566607-5566626 MS.gene21157:CDS 35.0%
GGTCATAAAAAGGGACATTT+AGG + chr5.1:5566357-5566376 None:intergenic 35.0%
GTATGTGTACAAGGATAAGA+AGG - chr5.1:5566879-5566898 MS.gene21157:CDS 35.0%
TACTAGTTAATTAGTGCGTG+TGG - chr5.1:5564732-5564751 MS.gene21157:CDS 35.0%
TCGATGACATCCTTCTTAAT+TGG - chr5.1:5566322-5566341 MS.gene21157:CDS 35.0%
TTCTTGAGCTCAAGATCTTT+GGG - chr5.1:5566691-5566710 MS.gene21157:CDS 35.0%
TTGTGAGAGACCTAATATCA+TGG + chr5.1:5564451-5564470 None:intergenic 35.0%
! AGATCCCATTAAGTGTTTTC+TGG - chr5.1:5566554-5566573 MS.gene21157:CDS 35.0%
! ATCAAGGACTATGAAAGTGT+TGG - chr5.1:5566100-5566119 MS.gene21157:intron 35.0%
! ATTCCTTACTGTTGCTTGAA+CGG + chr5.1:5564950-5564969 None:intergenic 35.0%
! CAATTTTGTCTTGTGTTGTG+GGG + chr5.1:5564359-5564378 None:intergenic 35.0%
! CTGATGGATAAAGCATACTA+TGG + chr5.1:5565072-5565091 None:intergenic 35.0%
! GACAATTTTGTCTTGTGTTG+TGG + chr5.1:5564361-5564380 None:intergenic 35.0%
! GGAGGCTTTATGATTATTAC+TGG - chr5.1:5566800-5566819 MS.gene21157:intron 35.0%
!! GTGTCTTTTTGTTTATCAGG+AGG - chr5.1:5564528-5564547 MS.gene21157:CDS 35.0%
!! TAAGTATCGGTACAAGAAAC+CGG - chr5.1:5565753-5565772 MS.gene21157:CDS 35.0%
!! TTTATGCTTTGTTTGTTCGG+TGG - chr5.1:5564675-5564694 MS.gene21157:CDS 35.0%
!!! GCACTTGTTGTGTTTTTATG+TGG - chr5.1:5565724-5565743 MS.gene21157:CDS 35.0%
AACTCCAGTATGCTGTAAAG+GGG + chr5.1:5566431-5566450 None:intergenic 40.0%
AAGATCATCAGGGTAGAGAT+TGG - chr5.1:5565668-5565687 MS.gene21157:CDS 40.0%
AATTGATCTGAACCAAAGCC+AGG + chr5.1:5565538-5565557 None:intergenic 40.0%
ACTAGGCATAACCGTGAATT+TGG - chr5.1:5564575-5564594 MS.gene21157:CDS 40.0%
AGATCATCAGGGTAGAGATT+GGG - chr5.1:5565669-5565688 MS.gene21157:CDS 40.0%
ATTGATCTGAACCAAAGCCA+GGG + chr5.1:5565537-5565556 None:intergenic 40.0%
CAACTCCAGTATGCTGTAAA+GGG + chr5.1:5566432-5566451 None:intergenic 40.0%
CAGATAATCTTAACCAAGGG+AGG - chr5.1:5566782-5566801 MS.gene21157:intron 40.0%
CGTAAACCTAGGATCTCTTA+TGG - chr5.1:5565621-5565640 MS.gene21157:CDS 40.0%
GATCCCACGATGAAAAATTG+CGG + chr5.1:5566586-5566605 None:intergenic 40.0%
GGGATGAAAACTATGAGCAT+GGG - chr5.1:5566664-5566683 MS.gene21157:CDS 40.0%
GTTAGGACTAATTGAGGGTT+TGG + chr5.1:5564827-5564846 None:intergenic 40.0%
GTTGTATGTTCCTTGCATGT+TGG + chr5.1:5565424-5565443 None:intergenic 40.0%
TAACAGGTCCAAGTAAACCA+TGG - chr5.1:5566755-5566774 MS.gene21157:intron 40.0%
TAGGGTGAGATGTAGTTAGT+TGG - chr5.1:5566012-5566031 MS.gene21157:intron 40.0%
TCTGCAATCCATGGTTTACT+TGG + chr5.1:5566766-5566785 None:intergenic 40.0%
TGCAAAGTGGTATGTGTACA+AGG - chr5.1:5566870-5566889 MS.gene21157:CDS 40.0%
TGGAAAACCCTTACAGTCAT+AGG + chr5.1:5564391-5564410 None:intergenic 40.0%
TGGGATGAAAACTATGAGCA+TGG - chr5.1:5566663-5566682 MS.gene21157:CDS 40.0%
TTAAGCACTTTCCTTGCAAC+TGG + chr5.1:5566391-5566410 None:intergenic 40.0%
TTCTCAGAGAATGTCCAGAT+GGG - chr5.1:5566644-5566663 MS.gene21157:CDS 40.0%
TTGAACAGTCCAAAACACGA+TGG + chr5.1:5566221-5566240 None:intergenic 40.0%
TTGCAACTGGACTAATGACA+CGG + chr5.1:5566378-5566397 None:intergenic 40.0%
TTTATCAGGAGGAGAAGTGA+TGG - chr5.1:5564539-5564558 MS.gene21157:CDS 40.0%
TTTCTAGGCGACACTTACAT+TGG - chr5.1:5564699-5564718 MS.gene21157:CDS 40.0%
TTTCTCAGAGAATGTCCAGA+TGG - chr5.1:5566643-5566662 MS.gene21157:CDS 40.0%
! AAAGATCTAAAACTGGCGGT+TGG - chr5.1:5564415-5564434 MS.gene21157:CDS 40.0%
! ACCATGGATTACCAGAATCA+CGG + chr5.1:5564800-5564819 None:intergenic 40.0%
! ATGCCGCAATTTTTCATCGT+GGG - chr5.1:5566580-5566599 MS.gene21157:CDS 40.0%
! ATTGAGGTATCTGTCACAGA+AGG + chr5.1:5564911-5564930 None:intergenic 40.0%
! CAATTTTTCATCGTGGGATC+AGG - chr5.1:5566586-5566605 MS.gene21157:CDS 40.0%
! CACTTCTTCTACATCGGTTA+AGG + chr5.1:5566161-5566180 None:intergenic 40.0%
! CCAGCAGAAAGATCTAAAAC+TGG - chr5.1:5564408-5564427 MS.gene21157:CDS 40.0%
! CTCATAGTTTTCATCCCATC+TGG + chr5.1:5566661-5566680 None:intergenic 40.0%
! GAACAGCTTTTTGGACAAAG+CGG - chr5.1:5566075-5566094 MS.gene21157:intron 40.0%
! GCAGAAAGATCTAAAACTGG+CGG - chr5.1:5564411-5564430 MS.gene21157:CDS 40.0%
!! AAGGACTATGAAAGTGTTGG+TGG - chr5.1:5566103-5566122 MS.gene21157:intron 40.0%
!! TAGTCCATTGACTCAGATTG+CGG - chr5.1:5565780-5565799 MS.gene21157:CDS 40.0%
!! TATACTTGGCTTGTGCAAAG+TGG - chr5.1:5566857-5566876 MS.gene21157:CDS 40.0%
!! TGGTTTGGTGAATGATCCAA+CGG - chr5.1:5566528-5566547 MS.gene21157:intron 40.0%
!!! CCAGTTTTAGATCTTTCTGC+TGG + chr5.1:5564411-5564430 None:intergenic 40.0%
AACCTCTGCTTCTTGATCCT+CGG + chr5.1:5565898-5565917 None:intergenic 45.0%
ACAACTATGGATCGCCACAT+TGG - chr5.1:5566274-5566293 MS.gene21157:intron 45.0%
ACCGTGATTCTGGTAATCCA+TGG - chr5.1:5564796-5564815 MS.gene21157:CDS 45.0%
AGGATCTCTTATGGCAGTGA+CGG - chr5.1:5565630-5565649 MS.gene21157:CDS 45.0%
ATCGTCGTTAAAGAGCAACG+CGG + chr5.1:5565857-5565876 None:intergenic 45.0%
ATGGAGAGGTTGACAACACT+AGG - chr5.1:5564558-5564577 MS.gene21157:CDS 45.0%
CAAGGATAAGAAGGTTGCTG+AGG - chr5.1:5566888-5566907 MS.gene21157:CDS 45.0%
CACGATGTTAACCGTGATTC+TGG - chr5.1:5564786-5564805 MS.gene21157:CDS 45.0%
CCAACTCCAGTATGCTGTAA+AGG + chr5.1:5566433-5566452 None:intergenic 45.0%
CCTGATGCCTATGACTGTAA+GGG - chr5.1:5564381-5564400 MS.gene21157:CDS 45.0%
CCTTACAGTCATAGGCATCA+GGG + chr5.1:5564383-5564402 None:intergenic 45.0%
CCTTTACAGCATACTGGAGT+TGG - chr5.1:5566430-5566449 MS.gene21157:intron 45.0%
CTGCAAAGACAGCGATATTG+AGG + chr5.1:5564927-5564946 None:intergenic 45.0%
CTTAATGGGATCTTTGCCGT+TGG + chr5.1:5566547-5566566 None:intergenic 45.0%
CTTACCCCTTTACAGCATAC+TGG - chr5.1:5566424-5566443 MS.gene21157:intron 45.0%
GATAAGAAGGTTGCTGAGGA+AGG - chr5.1:5566892-5566911 MS.gene21157:CDS 45.0%
GATCATCAGGGTAGAGATTG+GGG - chr5.1:5565670-5565689 MS.gene21157:CDS 45.0%
GCTAAGAATGCTCCCCATAT+GGG - chr5.1:5566189-5566208 MS.gene21157:intron 45.0%
GGTATCACAGGCAACAACTA+TGG - chr5.1:5566261-5566280 MS.gene21157:intron 45.0%
GTATGTTCCTTGCATGTTGG+AGG + chr5.1:5565421-5565440 None:intergenic 45.0%
TACCGCACAGCAAACAGTTT+CGG - chr5.1:5565917-5565936 MS.gene21157:intron 45.0%
TAGGCGACACTTACATTGGA+AGG - chr5.1:5564703-5564722 MS.gene21157:CDS 45.0%
TATCGGTACAAGAAACCGGT+AGG - chr5.1:5565757-5565776 MS.gene21157:CDS 45.0%
TCACTGCCATAAGAGATCCT+AGG + chr5.1:5565630-5565649 None:intergenic 45.0%
TGCTAAGAATGCTCCCCATA+TGG - chr5.1:5566188-5566207 MS.gene21157:intron 45.0%
! ACGATGGTTGATGCCCATAT+GGG + chr5.1:5566205-5566224 None:intergenic 45.0%
! GATGCCGCAATTTTTCATCG+TGG - chr5.1:5566579-5566598 MS.gene21157:CDS 45.0%
! GGTTTAAAATCCAGTGTCCC+TGG - chr5.1:5565517-5565536 MS.gene21157:CDS 45.0%
! GTGTCATTAGTCCAGTTGCA+AGG - chr5.1:5566377-5566396 MS.gene21157:intron 45.0%
! TGTTTGTTCGGTGGCTTTCT+AGG - chr5.1:5564684-5564703 MS.gene21157:CDS 45.0%
! TTGAGGGTTTGGTATCACCA+TGG + chr5.1:5564816-5564835 None:intergenic 45.0%
!! TACCGAAACTGTTTGCTGTG+CGG + chr5.1:5565922-5565941 None:intergenic 45.0%
!! TGAGTCAATGGACTACCTAC+CGG + chr5.1:5565775-5565794 None:intergenic 45.0%
AAATCCAGTGTCCCTGGCTT+TGG - chr5.1:5565523-5565542 MS.gene21157:CDS 50.0%
AAGAGCAACGCGGAATCAGA+AGG + chr5.1:5565847-5565866 None:intergenic 50.0%
AAGGATGTCATCGATGCTGC+TGG + chr5.1:5566316-5566335 None:intergenic 50.0%
ACGACCGCAATCTGAGTCAA+TGG + chr5.1:5565787-5565806 None:intergenic 50.0%
AGGATGTCATCGATGCTGCT+GGG + chr5.1:5566315-5566334 None:intergenic 50.0%
ATGTCACTGCACTTGGCACA+GGG - chr5.1:5565494-5565513 MS.gene21157:CDS 50.0%
CAGCCGTTCAAGCAACAGTA+AGG - chr5.1:5564944-5564963 MS.gene21157:CDS 50.0%
CAGGGTAGAGATTGGGGTTA+TGG - chr5.1:5565676-5565695 MS.gene21157:CDS 50.0%
CATGTTGGAGGACTTAGGCT+TGG + chr5.1:5565409-5565428 None:intergenic 50.0%
CCATCTTACATGAACCACCG+AGG - chr5.1:5565878-5565897 MS.gene21157:CDS 50.0%
CCCTGATGCCTATGACTGTA+AGG - chr5.1:5564380-5564399 MS.gene21157:CDS 50.0%
CCCTTACAGTCATAGGCATC+AGG + chr5.1:5564384-5564403 None:intergenic 50.0%
CCTAAGTCCTCCAACATGCA+AGG - chr5.1:5565411-5565430 MS.gene21157:CDS 50.0%
CCTCGGTGGTTCATGTAAGA+TGG + chr5.1:5565881-5565900 None:intergenic 50.0%
CCTTGCATGTTGGAGGACTT+AGG + chr5.1:5565414-5565433 None:intergenic 50.0%
GATGACACTCTCGGTATCAC+AGG - chr5.1:5566249-5566268 MS.gene21157:intron 50.0%
GCGCTTTATGTCACTGCACT+TGG - chr5.1:5565487-5565506 MS.gene21157:CDS 50.0%
TATGTCACTGCACTTGGCAC+AGG - chr5.1:5565493-5565512 MS.gene21157:CDS 50.0%
TCAGATTGCGGTCGTGTTTG+TGG - chr5.1:5565792-5565811 MS.gene21157:CDS 50.0%
TGTAAAGGGGTAAGCCCTTG+TGG + chr5.1:5566418-5566437 None:intergenic 50.0%
TTTGGACAAAGCGGCCATCA+AGG - chr5.1:5566084-5566103 MS.gene21157:intron 50.0%
! AAGGTTGCTGAGGAAGGCAT+GGG - chr5.1:5566898-5566917 MS.gene21157:CDS 50.0%
! CACGATGGTTGATGCCCATA+TGG + chr5.1:5566206-5566225 None:intergenic 50.0%
! CGATGGTTGATGCCCATATG+GGG + chr5.1:5566204-5566223 None:intergenic 50.0%
! GGGCATCAACCATCGTGTTT+TGG - chr5.1:5566209-5566228 MS.gene21157:intron 50.0%
CAAGTGCCTCTTCCTCCAAG+CGG + chr5.1:5565818-5565837 None:intergenic 55.0%
CACCGAGGATCAAGAAGCAG+AGG - chr5.1:5565893-5565912 MS.gene21157:intron 55.0%
CTCTGCTTCTTGATCCTCGG+TGG + chr5.1:5565895-5565914 None:intergenic 55.0%
GAAGGTTGCTGAGGAAGGCA+TGG - chr5.1:5566897-5566916 MS.gene21157:CDS 55.0%
TGAACCAAAGCCAGGGACAC+TGG + chr5.1:5565530-5565549 None:intergenic 55.0%
TGTCACTGCACTTGGCACAG+GGG - chr5.1:5565495-5565514 MS.gene21157:CDS 55.0%
! CAGGAGGAGAAGTGATGGAG+AGG - chr5.1:5564544-5564563 MS.gene21157:CDS 55.0%
GCAAGTTGCGGCGATCCTGA+TGG + chr5.1:5565088-5565107 None:intergenic 60.0%
GTCACTGCACTTGGCACAGG+GGG - chr5.1:5565496-5565515 MS.gene21157:CDS 60.0%
TCGTGTTTGTGGCCGCTTGG+AGG - chr5.1:5565803-5565822 MS.gene21157:CDS 60.0%
TTGTGGCCGCTTGGAGGAAG+AGG - chr5.1:5565809-5565828 MS.gene21157:CDS 60.0%
CGGTCGTGTTTGTGGCCGCT+TGG - chr5.1:5565800-5565819 MS.gene21157:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 5564345 5566927 5564345 ID=MS.gene21157
chr5.1 mRNA 5564345 5566927 5564345 ID=MS.gene21157.t1;Parent=MS.gene21157
chr5.1 exon 5566813 5566927 5566813 ID=MS.gene21157.t1.exon1;Parent=MS.gene21157.t1
chr5.1 CDS 5566813 5566927 5566813 ID=cds.MS.gene21157.t1;Parent=MS.gene21157.t1
chr5.1 exon 5566548 5566725 5566548 ID=MS.gene21157.t1.exon2;Parent=MS.gene21157.t1
chr5.1 CDS 5566548 5566725 5566548 ID=cds.MS.gene21157.t1;Parent=MS.gene21157.t1
chr5.1 exon 5566312 5566351 5566312 ID=MS.gene21157.t1.exon3;Parent=MS.gene21157.t1
chr5.1 CDS 5566312 5566351 5566312 ID=cds.MS.gene21157.t1;Parent=MS.gene21157.t1
chr5.1 exon 5565334 5565899 5565334 ID=MS.gene21157.t1.exon4;Parent=MS.gene21157.t1
chr5.1 CDS 5565334 5565899 5565334 ID=cds.MS.gene21157.t1;Parent=MS.gene21157.t1
chr5.1 exon 5564345 5565191 5564345 ID=MS.gene21157.t1.exon5;Parent=MS.gene21157.t1
chr5.1 CDS 5564345 5565191 5564345 ID=cds.MS.gene21157.t1;Parent=MS.gene21157.t1
Gene Sequence

>MS.gene21157

ATGGGCACTCTCCCCACAACACAAGACAAAATTGTCCCTGATGCCTATGACTGTAAGGGTTTTCCAGCAGAAAGATCTAAAACTGGCGGTTGGACTTCAGCTTCCATGATATTAGGTCTCTCACAAATTAATCAATCACTCTCTTCTACTGCATGCATGCCGTTTCTTTTGTATTGATTTTATGTGTCTTTTTGTTTATCAGGAGGAGAAGTGATGGAGAGGTTGACAACACTAGGCATAACCGTGAATTTGGTCACATATTTAACAGGTACAATGCATTTGGGTAACGCTGCCTCTGCCAACATAGTTACAAATTTTGTTGGAACTTCATTTATGCTTTGTTTGTTCGGTGGCTTTCTAGGCGACACTTACATTGGAAGGTTCGTATACTAGTTAATTAGTGCGTGTGGATGTTTGTTATCACGATGAGTTTGTCATAATCACGATGTTAACCGTGATTCTGGTAATCCATGGTGATACCAAACCCTCAATTAGTCCTAACAGTATCAATCAAATTTCTTTTTAGTTTCATTTCATTCAAGTGATATTTCGTTATTTTTGTTCCTTCTGTGACAGATACCTCAATATCGCTGTCTTTGCAGCCGTTCAAGCAACAGTAAGGAATCAAAACATGTTACTATTCATATTTGTTGCTAGCTACTAGTTTTGCTTTTACACCCAATTTAATAATCAACATTTCATTTTCATCTAAATATCTACACAACCATAGTATGCTTTATCCATCAGGATCGCCGCAACTTGCTATAAATTTATTATTATCTTTATACTTGTTCGATATTAGTAAGGTTTATTGTCTACCCCTTTGATTTTGTCTTTTGTATCTATAACACTATCTAAAGGGAATATGAGATTTTTGGCTCGATTTTCCTATAAAACAAATTTTCCTATAAAATAATTCATCATAATTTACACTACAGTAAAAAAAAGCGGAGAAATTGAACGCTAGTTTCTAATAATAATGATAATTTAAGAGTAGAATTTTGATAGTTTTATTCATAATTTAATAGGGTGTTGCTCTCTTGATGATATCAACTATCATTCCAAGCCTAAGTCCTCCAACATGCAAGGAACATACAACTTGTGTAAAAGCAAACAATACACAGTTAACGGTTCTGTTTTTAGCGCTTTATGTCACTGCACTTGGCACAGGGGGTTTAAAATCCAGTGTCCCTGGCTTTGGTTCAGATCAATTTGATGCTACTGATAAAGAAGAGAAGAAACATATGGTTAAATTTCTCAATTGGTATTACTTCATCGTAAACCTAGGATCTCTTATGGCAGTGACGGTCCTTGTTTACATACAAGATCATCAGGGTAGAGATTGGGGTTATGGTATCTGTGCTTGTGCTATTGTGCTAGCACTTGTTGTGTTTTTATGTGGAACATGTAAGTATCGGTACAAGAAACCGGTAGGTAGTCCATTGACTCAGATTGCGGTCGTGTTTGTGGCCGCTTGGAGGAAGAGGCACTTGCAATTGCCTTCTGATTCCGCGTTGCTCTTTAACGACGATGCCATCTTACATGAACCACCGAGGATCAAGAAGCAGAGGTTACCGCACAGCAAACAGTTTCGGTAACACTTTTTACCCTTGTATCTTATCGTAGATATTTGTAGATATATTTTATAAAGTGTCGGAATACTATAATAGGGTGAGATGTAGTTAGTTGGATGCATGTGTAAAGTTAACTGATAGTGTAAATATATTTTCGAACAGCTTTTTGGACAAAGCGGCCATCAAGGACTATGAAAGTGTTGGTGGAATCATGAAGATAAGTAAGTGGCATCTATCTACCTTAACCGATGTAGAAGAAGTGAAATTGGTGCTAAGAATGCTCCCCATATGGGCATCAACCATCGTGTTTTGGACTGTTCAAGTTCAAATGATGACACTCTCGGTATCACAGGCAACAACTATGGATCGCCACATTGGAAAATCATTTCAAATCCCAGCAGCATCGATGACATCCTTCTTAATTGGAACTATTCTCCTAAATGTCCCTTTTTATGACCGTGTCATTAGTCCAGTTGCAAGGAAAGTGCTTAAAAATCCACAAGGGCTTACCCCTTTACAGCATACTGGAGTTGGTTTAGTTCTTTCAATCTTATCTATGGTTGCAGCAGCTCTTGTCGAACAAAAGCGATTAACATTTGCACAATCACATGGTTTGGTGAATGATCCAACGGCAAAGATCCCATTAAGTGTTTTCTGGTTGATGCCGCAATTTTTCATCGTGGGATCAGGAGAGTCCTTTATGTATATGGGACAATTAGACTTTTTTCTCAGAGAATGTCCAGATGGGATGAAAACTATGAGCATGGGACTATTCTTGAGCTCAAGATCTTTGGGATTTTTCTTCAGCTCTTTATTAGTGTCCACAGTGAATAAAATAACAGGTCCAAGTAAACCATGGATTGCAGATAATCTTAACCAAGGGAGGCTTTATGATTATTACTGGCTTTTGGCTATGTTGAGTGCTATAAATTTGATGATATACTTGGCTTGTGCAAAGTGGTATGTGTACAAGGATAAGAAGGTTGCTGAGGAAGGCATGGGAGAGTAA
Protein sequence

>MS.gene21157.t1

MGTLPTTQDKIVPDAYDCKGFPAERSKTGGWTSASMILGGEVMERLTTLGITVNLVTYLTGTMHLGNAASANIVTNFVGTSFMLCLFGGFLGDTYIGRYLNIAVFAAVQATGVALLMISTIIPSLSPPTCKEHTTCVKANNTQLTVLFLALYVTALGTGGLKSSVPGFGSDQFDATDKEEKKHMVKFLNWYYFIVNLGSLMAVTVLVYIQDHQGRDWGYGICACAIVLALVVFLCGTCKYRYKKPVGSPLTQIAVVFVAAWRKRHLQLPSDSALLFNDDAILHEPPRIKKQRLPHSKQFRFLDKAAIKDYESVGGIMKISKWHLSTLTDVEEVKLVLRMLPIWASTIVFWTVQVQMMTLSVSQATTMDRHIGKSFQIPAASMTSFLIGTILLNVPFYDRVISPVARKVLKNPQGLTPLQHTGVGLVLSILSMVAAALVEQKRLTFAQSHGLVNDPTAKIPLSVFWLMPQFFIVGSGESFMYMGQLDFFLRECPDGMKTMSMGLFLSSRSLGFFFSSLLVSTVNKITGPSKPWIADNLNQGRLYDYYWLLAMLSAINLMIYLACAKWYVYKDKKVAEEGMGE