AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79483

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79483.t1 MTR_4g011280 96.672 601 20 0 1 601 1 601 0.0 1206
MS.gene79483.t1 MTR_2g101640 70.326 583 169 2 15 593 6 588 0.0 863
MS.gene79483.t1 MTR_2g101590 70.018 567 167 2 18 582 13 578 0.0 818
MS.gene79483.t1 MTR_2g101610 67.802 587 176 5 1 582 1 579 0.0 806
MS.gene79483.t1 MTR_2g101560 66.781 581 188 2 18 593 13 593 0.0 779
MS.gene79483.t1 MTR_1g057460 66.667 561 169 4 18 578 31 573 0.0 766
MS.gene79483.t1 MTR_2g101650 58.475 590 233 5 1 588 1 580 0.0 731
MS.gene79483.t1 MTR_1g057460 67.052 519 154 3 60 578 1 502 0.0 715
MS.gene79483.t1 MTR_0236s0050 67.630 519 151 4 60 578 1 502 0.0 699
MS.gene79483.t1 MTR_8g103233 50.085 587 277 9 18 601 6 579 0.0 572
MS.gene79483.t1 MTR_1g026740 48.474 557 276 6 19 571 6 555 0.0 537
MS.gene79483.t1 MTR_1g009200 49.389 573 278 7 13 582 11 574 0.0 535
MS.gene79483.t1 MTR_1g026750 48.741 556 274 6 33 582 19 569 7.43e-180 521
MS.gene79483.t1 MTR_1g026770 47.273 550 280 6 33 578 16 559 8.47e-174 505
MS.gene79483.t1 MTR_8g469480 37.900 562 331 8 35 584 41 596 5.16e-132 399
MS.gene79483.t1 MTR_5g038060 35.902 610 363 12 2 598 4 598 7.25e-132 399
MS.gene79483.t1 MTR_4g107510 36.097 579 355 10 2 569 7 581 7.38e-130 394
MS.gene79483.t1 MTR_5g055000 38.949 552 313 10 31 569 17 557 4.08e-128 389
MS.gene79483.t1 MTR_4g107580 34.872 585 360 9 13 585 5 580 1.86e-127 387
MS.gene79483.t1 MTR_7g088790 34.899 596 363 11 5 585 2 587 3.62e-118 364
MS.gene79483.t1 MTR_4g107530 34.813 563 353 9 32 583 33 592 7.48e-118 363
MS.gene79483.t1 MTR_5g038190 34.046 561 353 11 20 569 11 565 5.34e-115 355
MS.gene79483.t1 MTR_8g469470 34.483 580 360 11 5 569 4 578 1.14e-113 352
MS.gene79483.t1 MTR_7g088825 32.542 590 372 14 27 599 17 597 1.81e-112 349
MS.gene79483.t1 MTR_7g088820 32.815 579 369 10 26 593 20 589 7.98e-109 339
MS.gene79483.t1 MTR_4g107610 30.328 610 397 11 1 590 1 602 2.22e-102 323
MS.gene79483.t1 MTR_3g462710 34.128 545 340 10 34 569 26 560 1.02e-100 317
MS.gene79483.t1 MTR_1g009170 41.244 434 191 10 155 585 5 377 6.75e-99 306
MS.gene79483.t1 MTR_7g067010 34.522 533 326 9 41 563 33 552 2.32e-98 311
MS.gene79483.t1 MTR_5g012270 31.208 596 364 14 17 585 6 582 8.67e-98 310
MS.gene79483.t1 MTR_7g088820 32.083 533 343 9 72 593 3 527 1.57e-97 308
MS.gene79483.t1 MTR_7g064780 29.877 569 377 9 31 586 25 584 2.72e-97 309
MS.gene79483.t1 MTR_4g015080 33.272 544 344 10 34 569 36 568 9.57e-97 307
MS.gene79483.t1 MTR_7g064800 31.065 573 361 13 13 563 3 563 2.29e-95 304
MS.gene79483.t1 MTR_3g112460 33.034 557 331 16 27 566 15 546 1.30e-92 296
MS.gene79483.t1 MTR_3g112450 33.214 560 342 13 31 578 22 561 3.63e-91 292
MS.gene79483.t1 MTR_4g114340 32.717 541 353 9 34 571 26 558 2.42e-90 290
MS.gene79483.t1 MTR_5g085850 31.707 574 346 15 34 590 32 576 2.70e-90 291
MS.gene79483.t1 MTR_5g012290 30.159 567 371 10 34 585 29 585 2.86e-90 291
MS.gene79483.t1 MTR_3g112470 33.150 546 328 15 34 566 25 546 5.63e-90 289
MS.gene79483.t1 MTR_2g093160 33.454 553 324 15 32 569 21 544 9.29e-90 288
MS.gene79483.t1 MTR_4g033325 34.239 552 326 12 27 569 25 548 1.59e-89 288
MS.gene79483.t1 MTR_4g033315 32.990 582 354 12 2 575 3 556 1.41e-88 285
MS.gene79483.t1 MTR_4g033310 32.990 582 354 12 2 575 3 556 1.41e-88 285
MS.gene79483.t1 MTR_4g101380 30.523 593 392 12 8 585 1 588 5.27e-88 285
MS.gene79483.t1 MTR_0294s0020 32.911 553 332 12 27 569 25 548 4.11e-87 281
MS.gene79483.t1 MTR_7g088820 31.417 487 315 9 118 593 1 479 4.44e-87 279
MS.gene79483.t1 MTR_7g098200 31.746 567 352 12 32 587 29 571 8.41e-87 281
MS.gene79483.t1 MTR_7g098200 31.533 574 354 12 32 587 29 581 9.68e-87 281
MS.gene79483.t1 MTR_2g093180 33.575 551 327 13 32 569 21 545 1.21e-85 277
MS.gene79483.t1 MTR_2g093640 33.216 566 342 11 15 569 4 544 1.22e-85 277
MS.gene79483.t1 MTR_7g098040 30.664 587 382 11 1 575 2 575 2.58e-85 278
MS.gene79483.t1 MTR_3g072300 31.295 556 354 14 27 569 18 558 1.67e-84 275
MS.gene79483.t1 MTR_7g098160 30.102 588 392 11 6 583 4 582 2.01e-84 276
MS.gene79483.t1 MTR_3g069500 30.419 549 353 10 28 569 31 557 3.93e-84 274
MS.gene79483.t1 MTR_4g019580 31.397 551 352 14 41 582 45 578 6.36e-84 274
MS.gene79483.t1 MTR_7g098220 31.455 550 362 10 27 569 24 565 5.18e-83 272
MS.gene79483.t1 MTR_4g033330 33.835 532 321 11 27 552 25 531 5.62e-83 270
MS.gene79483.t1 MTR_3g005010 31.065 573 350 11 13 573 7 546 7.23e-83 270
MS.gene79483.t1 MTR_7g098180 31.338 568 376 10 6 566 4 564 8.00e-83 271
MS.gene79483.t1 MTR_7g098230 31.694 549 358 11 46 587 40 578 1.94e-82 270
MS.gene79483.t1 MTR_3g462710 33.839 461 286 10 118 569 1 451 5.83e-82 265
MS.gene79483.t1 MTR_7g098090 30.329 577 366 12 27 583 27 587 4.43e-81 267
MS.gene79483.t1 MTR_7g006540 32.042 568 339 13 30 569 61 609 9.02e-81 267
MS.gene79483.t1 MTR_1g075440 30.944 572 370 11 27 586 22 580 9.84e-81 266
MS.gene79483.t1 MTR_7g098220 31.559 526 346 9 50 569 4 521 1.35e-80 264
MS.gene79483.t1 MTR_1g042310 30.261 575 370 13 27 586 20 578 1.59e-80 265
MS.gene79483.t1 MTR_3g069420 30.965 549 360 9 28 569 30 566 2.12e-80 265
MS.gene79483.t1 MTR_7g065080 30.364 550 354 13 28 569 27 555 4.07e-80 264
MS.gene79483.t1 MTR_2g005130 29.338 559 364 12 31 581 39 574 2.35e-79 262
MS.gene79483.t1 MTR_3g069440 29.636 550 366 9 28 569 29 565 1.09e-78 260
MS.gene79483.t1 MTR_1g042310 30.325 554 356 12 47 586 4 541 7.58e-78 257
MS.gene79483.t1 MTR_3g100980 30.928 582 366 15 23 589 6 566 2.68e-77 256
MS.gene79483.t1 MTR_7g005930 30.796 565 365 11 31 585 34 582 3.71e-77 256
MS.gene79483.t1 MTR_5g014150 28.378 592 406 11 3 583 15 599 8.80e-77 255
MS.gene79483.t1 MTR_2g017750 28.571 602 405 15 1 582 1 597 1.00e-76 255
MS.gene79483.t1 MTR_1g042310 30.364 550 353 12 51 586 1 534 1.05e-76 254
MS.gene79483.t1 MTR_8g038880 30.796 565 343 10 18 563 6 541 1.18e-76 254
MS.gene79483.t1 MTR_4g033325 33.902 469 274 11 109 569 10 450 1.24e-76 251
MS.gene79483.t1 MTR_1g041875 30.605 562 363 12 34 586 31 574 1.47e-76 254
MS.gene79483.t1 MTR_4g033375 32.530 498 304 10 27 517 26 498 8.26e-76 250
MS.gene79483.t1 MTR_4g033395 33.533 501 295 10 27 517 26 498 1.19e-75 249
MS.gene79483.t1 MTR_7g065080 31.288 489 307 13 89 569 1 468 1.37e-75 249
MS.gene79483.t1 MTR_4g033325 33.937 442 265 10 136 569 2 424 3.10e-75 246
MS.gene79483.t1 MTR_1g041815 30.017 573 373 14 27 586 21 578 4.64e-75 251
MS.gene79483.t1 MTR_1g041835 29.463 577 371 15 27 586 21 578 2.72e-74 248
MS.gene79483.t1 MTR_1g041885 30.469 512 327 13 51 552 1 493 5.68e-74 246
MS.gene79483.t1 MTR_1g085090 27.778 576 387 13 32 599 19 573 7.16e-74 248
MS.gene79483.t1 MTR_7g013270 30.335 567 369 9 33 586 91 644 4.30e-72 244
MS.gene79483.t1 MTR_3g100980 29.672 610 365 16 23 589 6 594 1.41e-71 241
MS.gene79483.t1 MTR_4g033385 32.759 464 273 10 117 569 16 451 1.48e-71 238
MS.gene79483.t1 MTR_8g087780 31.015 532 342 14 31 543 20 545 3.30e-71 240
MS.gene79483.t1 MTR_3g014790 28.425 584 359 12 25 586 10 556 4.26e-71 239
MS.gene79483.t1 MTR_4g098800 29.346 535 357 11 42 566 46 569 6.12e-71 239
MS.gene79483.t1 MTR_7g098200 31.557 488 295 12 118 587 1 467 7.61e-71 236
MS.gene79483.t1 MTR_4g098800 29.346 535 357 11 42 566 111 634 1.87e-70 240
MS.gene79483.t1 MTR_8g469310 30.357 560 343 15 34 568 28 565 4.56e-69 234
MS.gene79483.t1 MTR_8g087810 30.319 564 368 12 31 572 20 580 1.92e-68 233
MS.gene79483.t1 MTR_1g116930 28.496 565 375 13 31 586 22 566 1.37e-67 231
MS.gene79483.t1 MTR_5g038380 27.993 568 364 11 26 568 21 568 2.41e-67 230
MS.gene79483.t1 MTR_4g107670 29.130 563 352 15 32 568 24 565 2.73e-67 229
MS.gene79483.t1 MTR_7g010820 29.287 519 350 10 42 549 36 548 4.21e-66 226
MS.gene79483.t1 MTR_7g111260 28.092 566 371 14 31 588 18 555 4.53e-66 226
MS.gene79483.t1 MTR_4g123537 27.526 574 374 17 25 586 37 580 1.17e-56 201
MS.gene79483.t1 MTR_6g008690 28.649 555 340 14 41 572 16 537 2.40e-56 199
MS.gene79483.t1 MTR_7g098210 28.736 522 268 14 70 575 12 445 3.80e-53 188
MS.gene79483.t1 MTR_4g033310 31.627 332 210 5 248 575 174 492 2.90e-51 184
MS.gene79483.t1 MTR_4g033310 34.848 198 114 5 2 198 3 186 5.34e-18 87.8
MS.gene79483.t1 MTR_7g098150 28.446 457 301 12 6 449 4 447 4.58e-51 183
MS.gene79483.t1 MTR_4g024930 25.342 584 389 16 1 569 1 552 5.71e-46 171
MS.gene79483.t1 MTR_7g052600 24.178 517 341 11 60 569 53 525 1.67e-35 140
MS.gene79483.t1 MTR_7g051880 24.510 510 336 11 60 563 53 519 6.23e-33 133
MS.gene79483.t1 MTR_7g051830 24.266 511 336 12 60 563 53 519 2.89e-32 131
MS.gene79483.t1 MTR_8g091265 40.367 109 59 4 299 403 3 109 1.51e-17 80.1
MS.gene79483.t1 MTR_3g065350 27.879 165 109 4 341 503 1 157 4.41e-14 70.9
MS.gene79483.t1 MTR_4g033390 34.043 94 59 2 487 577 30 123 6.60e-12 63.5
MS.gene79483.t1 MTR_7g064160 28.571 84 60 0 33 116 30 113 1.55e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79483.t1 AT1G52190 53.897 603 264 7 8 600 1 599 0.0 682
MS.gene79483.t1 AT3G16180 54.454 595 252 10 8 588 1 590 0.0 669
MS.gene79483.t1 AT1G69870 36.318 592 350 13 12 586 32 613 2.54e-131 398
MS.gene79483.t1 AT1G68570 37.081 596 344 12 16 588 1 588 9.55e-122 373
MS.gene79483.t1 AT5G62680 34.150 612 372 12 1 586 1 607 1.16e-121 373
MS.gene79483.t1 AT5G11570 38.674 543 249 11 33 570 14 477 1.16e-114 350
MS.gene79483.t1 AT3G47960 34.310 580 365 10 1 569 31 605 4.77e-114 354
MS.gene79483.t1 AT1G68570 36.702 564 328 11 44 588 5 558 5.87e-114 352
MS.gene79483.t1 AT1G27080 33.391 575 357 10 34 599 16 573 2.63e-112 348
MS.gene79483.t1 AT1G18880 34.369 547 342 10 35 569 22 563 6.46e-111 344
MS.gene79483.t1 AT1G62200 35.915 568 332 12 14 565 30 581 3.88e-106 332
MS.gene79483.t1 AT1G27080 33.394 548 339 10 61 599 1 531 3.56e-105 328
MS.gene79483.t1 AT5G28470 32.628 567 351 12 5 562 2 546 1.45e-104 327
MS.gene79483.t1 AT2G02040 35.466 547 330 12 28 562 36 571 9.55e-103 323
MS.gene79483.t1 AT1G62200 34.338 597 331 12 14 565 30 610 4.50e-102 322
MS.gene79483.t1 AT3G54140 33.640 544 344 8 34 569 26 560 5.69e-102 321
MS.gene79483.t1 AT3G54140 33.640 544 344 8 34 569 26 560 5.69e-102 321
MS.gene79483.t1 AT5G28470 32.451 567 350 13 5 562 2 544 1.02e-101 320
MS.gene79483.t1 AT1G62200 34.338 597 331 12 14 565 44 624 1.12e-101 322
MS.gene79483.t1 AT3G45650 33.581 539 333 9 34 569 24 540 3.94e-98 310
MS.gene79483.t1 AT2G26690 33.155 561 361 10 34 590 26 576 7.94e-97 307
MS.gene79483.t1 AT5G01180 32.971 552 354 11 24 567 16 559 2.65e-96 306
MS.gene79483.t1 AT5G01180 32.971 552 354 11 24 567 16 559 2.65e-96 306
MS.gene79483.t1 AT5G01180 32.971 552 354 11 24 567 16 559 2.65e-96 306
MS.gene79483.t1 AT1G69860 34.004 547 328 11 35 569 21 546 1.24e-94 301
MS.gene79483.t1 AT2G26690 32.969 549 354 10 46 590 5 543 5.80e-94 299
MS.gene79483.t1 AT3G45650 32.035 565 333 10 34 569 24 566 1.54e-92 296
MS.gene79483.t1 AT3G45660 32.965 543 337 10 31 569 20 539 2.21e-92 295
MS.gene79483.t1 AT3G45660 32.965 543 337 10 31 569 20 539 2.21e-92 295
MS.gene79483.t1 AT3G45660 32.963 540 335 10 34 569 44 560 5.56e-92 295
MS.gene79483.t1 AT2G26690 32.902 541 349 10 54 590 1 531 1.10e-91 293
MS.gene79483.t1 AT3G21670 31.787 582 360 14 28 596 25 582 7.00e-91 292
MS.gene79483.t1 AT2G02020 32.482 548 319 12 28 569 38 540 2.23e-90 290
MS.gene79483.t1 AT3G45720 30.551 563 353 12 32 587 19 550 3.56e-88 284
MS.gene79483.t1 AT3G45680 31.434 579 361 11 16 587 4 553 1.30e-87 283
MS.gene79483.t1 AT5G46040 31.681 565 361 9 41 594 36 586 7.75e-87 281
MS.gene79483.t1 AT3G45710 30.142 564 359 10 31 587 20 555 1.02e-84 275
MS.gene79483.t1 AT1G12110 32.105 570 362 12 34 588 29 588 2.51e-83 272
MS.gene79483.t1 AT1G33440 32.174 575 352 14 27 586 35 586 8.78e-83 271
MS.gene79483.t1 AT5G13400 31.993 572 360 11 29 585 61 618 9.10e-83 272
MS.gene79483.t1 AT3G54450 29.577 568 362 10 31 583 11 555 1.60e-82 269
MS.gene79483.t1 AT5G46050 29.558 565 373 8 33 586 28 578 8.73e-82 268
MS.gene79483.t1 AT3G53960 28.620 587 384 13 1 569 1 570 1.30e-80 266
MS.gene79483.t1 AT1G59740 30.068 592 382 16 7 583 15 589 2.03e-79 262
MS.gene79483.t1 AT3G45700 29.701 569 353 13 34 593 18 548 2.39e-79 261
MS.gene79483.t1 AT2G40460 30.619 565 358 16 27 578 18 561 1.25e-78 260
MS.gene79483.t1 AT1G22540 31.308 543 345 8 32 569 30 549 2.67e-78 258
MS.gene79483.t1 AT2G37900 29.250 547 359 11 28 566 34 560 1.26e-76 254
MS.gene79483.t1 AT1G32450 27.835 582 400 10 32 601 37 610 1.75e-75 252
MS.gene79483.t1 AT1G72125 29.981 537 347 9 41 569 38 553 2.23e-75 251
MS.gene79483.t1 AT1G27040 29.927 548 350 11 34 568 29 555 1.27e-74 249
MS.gene79483.t1 AT1G27040 29.927 548 350 11 34 568 25 551 1.34e-74 249
MS.gene79483.t1 AT1G72140 29.891 552 337 10 32 569 34 549 1.86e-74 248
MS.gene79483.t1 AT2G02020 30.092 545 315 12 28 569 38 519 5.68e-73 243
MS.gene79483.t1 AT1G27040 29.926 538 343 11 44 568 7 523 4.46e-72 241
MS.gene79483.t1 AT2G37900 29.448 489 317 11 86 566 13 481 1.88e-71 238
MS.gene79483.t1 AT1G22570 29.423 537 333 8 32 550 30 538 3.04e-70 237
MS.gene79483.t1 AT5G46050 30.417 480 309 8 118 586 1 466 6.73e-70 234
MS.gene79483.t1 AT4G21680 28.777 556 365 11 32 569 28 570 2.62e-69 235
MS.gene79483.t1 AT3G45690 29.412 544 313 14 32 569 18 496 4.45e-69 233
MS.gene79483.t1 AT1G22550 29.211 558 344 12 32 569 30 556 1.34e-68 233
MS.gene79483.t1 AT5G14940 29.107 560 353 14 42 582 17 551 2.11e-68 232
MS.gene79483.t1 AT1G22570 29.254 523 324 8 46 550 7 501 8.27e-68 229
MS.gene79483.t1 AT1G72130 28.962 549 338 11 31 569 24 530 1.78e-66 226
MS.gene79483.t1 AT1G72120 30.037 546 349 9 32 569 29 549 5.20e-66 226
MS.gene79483.t1 AT5G19640 27.679 560 364 12 33 579 60 591 1.09e-64 223
MS.gene79483.t1 AT1G69850 28.700 554 369 11 34 568 27 573 5.30e-64 221
MS.gene79483.t1 AT3G01350 29.443 574 349 17 42 587 17 562 4.52e-63 218
MS.gene79483.t1 AT3G25260 27.536 552 336 14 27 568 15 512 1.12e-58 205
MS.gene79483.t1 AT5G62730 27.709 563 355 13 26 568 49 579 5.80e-53 191
MS.gene79483.t1 AT1G72130 29.002 431 264 9 149 569 14 412 4.23e-51 182
MS.gene79483.t1 AT1G72130 29.002 431 264 9 149 569 14 412 4.23e-51 182
MS.gene79483.t1 AT5G62730 27.605 547 345 12 41 568 6 520 1.34e-50 183
MS.gene79483.t1 AT3G25280 26.848 514 329 12 59 568 48 518 2.85e-50 182
MS.gene79483.t1 AT2G38100 23.247 542 359 21 60 586 2 501 4.09e-21 97.4
MS.gene79483.t1 AT2G38100 23.432 542 358 21 60 586 24 523 1.32e-20 95.9
MS.gene79483.t1 AT2G38100 23.247 542 359 21 60 586 18 517 1.49e-20 95.9

Find 131 sgRNAs with CRISPR-Local

Find 207 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAATTTCATGCCTCTTAT+TGG 0.106772 4.2:-89161128 MS.gene79483:CDS
TCCAATGCCCTTTGGTTATC+AGG 0.181681 4.2:+89160015 None:intergenic
CGGTGTAGTAGAACTCATTT+TGG 0.201381 4.2:+89158969 None:intergenic
ATCAAATAAAGTATCATATT+TGG 0.223029 4.2:+89161212 None:intergenic
GGCTGGAACTTCGAAGTTTC+GGG 0.229045 4.2:+89159303 None:intergenic
TCATTTCTGAAGTCAATTAA+TGG 0.245568 4.2:+89158659 None:intergenic
CGGTTCCTTGCTGTTGGTTT+AGG 0.251282 4.2:-89161077 MS.gene79483:CDS
CTTAACATTGGAGGCTCATT+TGG 0.269927 4.2:-89159361 MS.gene79483:CDS
CTGTTCCATCTGATAAAATA+AGG 0.270293 4.2:-89159690 MS.gene79483:intron
TTCATTGCTGATTCTCATCT+TGG 0.271738 4.2:-89161101 MS.gene79483:CDS
GCTGAAGCATTCAATGTTAT+AGG 0.290021 4.2:-89158992 MS.gene79483:CDS
TGATATTCACAATATTTATA+TGG 0.291881 4.2:-89159261 MS.gene79483:CDS
CTTGGATGGAAACTTGGTTT+TGG 0.310808 4.2:-89159910 MS.gene79483:CDS
ACAAATAGCACCTTGTTTAC+CGG 0.313397 4.2:-89159814 MS.gene79483:CDS
CTTGCTAGAGTTGCAACATT+AGG 0.322247 4.2:-89161242 MS.gene79483:CDS
GTAGTATGTAGTTGGGCTTA+TGG 0.330481 4.2:-89158731 MS.gene79483:CDS
ACCAATAAGAGGCATGAAAT+TGG 0.330763 4.2:+89161127 None:intergenic
CGGCTGGAACTTCGAAGTTT+CGG 0.331908 4.2:+89159302 None:intergenic
TGGTGGTTGCAACAGATAAA+AGG 0.339836 4.2:+89161147 None:intergenic
ATCACCATGCCTTTCATCAT+AGG 0.343097 4.2:-89161454 MS.gene79483:intron
AGGTTCCATCATCACTTTCA+TGG 0.346117 4.2:-89161057 MS.gene79483:intron
TCCCTTGCTCTTATGTCCAT+TGG 0.348657 4.2:-89160099 MS.gene79483:CDS
AAATATGATACTTTATTTGA+TGG 0.352112 4.2:-89161210 MS.gene79483:CDS
CTTCAATGCTCCATAGCGTT+TGG 0.357999 4.2:-89160066 MS.gene79483:CDS
CAGCAATTGTCGAGACTATA+AGG 0.361104 4.2:-89159114 MS.gene79483:CDS
TACATTCCAGCCGAGTTCTT+AGG 0.374613 4.2:+89159739 None:intergenic
TGGAAATGATGGATGGATAT+CGG 0.386093 4.2:-89158831 MS.gene79483:CDS
GTTTCCATCCAAGATGATCT+TGG 0.407018 4.2:+89159920 None:intergenic
GCCGATTCGACAAGTACTAT+TGG 0.414464 4.2:-89158793 MS.gene79483:CDS
TGTATTATGTAGTATGTAGT+TGG 0.415728 4.2:-89158739 MS.gene79483:CDS
AGGTCTTGCTTGTGGGATCA+TGG 0.416917 4.2:+89160191 None:intergenic
TGACATAGTCTTGGGAAATT+CGG 0.419062 4.2:+89158949 None:intergenic
GATCATCTTGGATGGAAACT+TGG 0.426344 4.2:-89159916 MS.gene79483:CDS
CTTGGACGGTTCCTTGCTGT+TGG 0.429953 4.2:-89161083 MS.gene79483:CDS
TTTCCTCAACTTTGCTTGGG+TGG 0.439391 4.2:-89159019 MS.gene79483:CDS
GCTCTTATGTCCATTGGAAA+TGG 0.445474 4.2:-89160093 MS.gene79483:CDS
ATGGATCACCTTCAGATCCT+TGG 0.446076 4.2:-89159468 MS.gene79483:CDS
AGAATATTTCCAATGCCCTT+TGG 0.446255 4.2:+89160007 None:intergenic
AATATGATACTTTATTTGAT+GGG 0.446790 4.2:-89161209 MS.gene79483:CDS
GATTAGTGCCAAGGCAAGAA+TGG 0.447421 4.2:-89159176 MS.gene79483:CDS
GCCAATAGTACTTGTCGAAT+CGG 0.449366 4.2:+89158792 None:intergenic
GCAATGAAAGCACCAATAAG+AGG 0.457008 4.2:+89161116 None:intergenic
CGACAATTGCTGCAGTTACC+AGG 0.459712 4.2:+89159125 None:intergenic
ATCTGCAACAACTTCACAAA+TGG 0.461858 4.2:-89160136 MS.gene79483:CDS
CTCGAAGCTCAGAGGAAAAC+CGG 0.462836 4.2:-89159203 MS.gene79483:CDS
TCCGAGTCCCATTCTTGCCT+TGG 0.463487 4.2:+89159168 None:intergenic
CCCTGATAACCAAAGGGCAT+TGG 0.463599 4.2:-89160016 MS.gene79483:CDS
ACCTCGAGAGGTGGAAATGA+TGG 0.466678 4.2:-89158842 MS.gene79483:CDS
AAGGAGGAGGTCTTGCTTGT+GGG 0.467412 4.2:+89160184 None:intergenic
CCACCCTTGTTGTGTAATTG+AGG 0.471804 4.2:+89161481 None:intergenic
CATGATTACACTAAGTGAAC+CGG 0.476193 4.2:+89159282 None:intergenic
TTTATTCAGCATTGTCGAAA+GGG 0.476280 4.2:-89158867 MS.gene79483:CDS
ATTAATTGACTTCAGAAATG+AGG 0.483313 4.2:-89158657 MS.gene79483:CDS
CAAACGCTATGGAGCATTGA+AGG 0.485367 4.2:+89160067 None:intergenic
GTATTATGTAGTATGTAGTT+GGG 0.485998 4.2:-89158738 MS.gene79483:CDS
GAAAAGAACATAAGTGCTGC+TGG 0.488631 4.2:+89159883 None:intergenic
ATCAAAAGTAAGTGAAGATG+AGG 0.488823 4.2:-89158690 MS.gene79483:CDS
CCAATGCCCTTTGGTTATCA+GGG 0.489270 4.2:+89160016 None:intergenic
CTGGTCCGCGCCAAACGCTA+TGG 0.494260 4.2:+89160056 None:intergenic
CATAGCGTTTGGCGCGGACC+AGG 0.495367 4.2:-89160055 MS.gene79483:CDS
CATTGGAAATATTCTTCAGT+TGG 0.496292 4.2:-89159999 MS.gene79483:CDS
TCTGTCATAATTGCATTCAC+AGG 0.501051 4.2:-89159955 MS.gene79483:CDS
GCCTTGGCACTAATCCTGAC+CGG 0.501561 4.2:+89159184 None:intergenic
AGCGATGCTTGACATAGTCT+TGG 0.506861 4.2:+89158940 None:intergenic
AGCTCAGAGGAAAACCGGTC+AGG 0.511679 4.2:-89159198 MS.gene79483:CDS
TGGCTTATTGCCACAGTGAG+TGG 0.513020 4.2:-89158773 MS.gene79483:CDS
ACTTACCATGAAAGTGATGA+TGG 0.513494 4.2:+89161052 None:intergenic
GCGATGCTTGACATAGTCTT+GGG 0.515053 4.2:+89158941 None:intergenic
ATTAGTGCCAAGGCAAGAAT+GGG 0.517734 4.2:-89159175 MS.gene79483:CDS
TTGCTGATTCTCATCTTGGA+CGG 0.525595 4.2:-89161097 MS.gene79483:CDS
TTGATGTCACTTAACATTGG+AGG 0.525633 4.2:-89159370 MS.gene79483:CDS
CAGTTGGTACTATGCTTCCT+CGG 0.529829 4.2:-89159983 MS.gene79483:CDS
TTAATTGACTTCAGAAATGA+GGG 0.531298 4.2:-89158656 MS.gene79483:CDS
CAAGGAGGAGGTCTTGCTTG+TGG 0.537281 4.2:+89160183 None:intergenic
GACATGTTCAACACTGAATG+AGG 0.541800 4.2:+89159055 None:intergenic
CTACCACCTAAGAACTCGGC+TGG 0.541857 4.2:-89159745 MS.gene79483:CDS
GTATATATCCAAGATCATCT+TGG 0.542980 4.2:-89159928 MS.gene79483:CDS
AGAAGATATAATGTATCCTA+CGG 0.552827 4.2:+89158892 None:intergenic
ATATGATACTTTATTTGATG+GGG 0.555276 4.2:-89161208 MS.gene79483:CDS
AGAAGTGAACCTATGATGAA+AGG 0.564424 4.2:+89161445 None:intergenic
TTTGATTCTACATCAGCAGT+AGG 0.565843 4.2:+89158707 None:intergenic
CTTATGTCCATTGGAAATGG+TGG 0.567721 4.2:-89160090 MS.gene79483:CDS
TTGAAGGCCACCATTTCCAA+TGG 0.569645 4.2:+89160083 None:intergenic
ACCGGTCAGGATTAGTGCCA+AGG 0.571104 4.2:-89159185 MS.gene79483:CDS
TCGTACAAAGCCGGTAAACA+AGG 0.571512 4.2:+89159804 None:intergenic
ATCTTGATGTCACTTAACAT+TGG 0.572463 4.2:-89159373 MS.gene79483:CDS
CCTCTGAGCTTCGAGGCTAG+AGG 0.574416 4.2:+89159211 None:intergenic
ACAACCTCAATTACACAACA+AGG 0.575178 4.2:-89161485 MS.gene79483:CDS
CGAGAGGTGGAAATGATGGA+TGG 0.575410 4.2:-89158838 MS.gene79483:CDS
AACGGTCACTCGTACAAAGC+CGG 0.581357 4.2:+89159795 None:intergenic
TTATTAAAGTTATTCCATTG+TGG 0.581425 4.2:-89159405 MS.gene79483:CDS
AGATAACCCTGATAACCAAA+GGG 0.582920 4.2:-89160022 MS.gene79483:CDS
TCCATCATTTCCACCTCTCG+AGG 0.582933 4.2:+89158841 None:intergenic
ATATCCAAGATCATCTTGGA+TGG 0.583794 4.2:-89159924 MS.gene79483:CDS
AGAGCAATCAGTGAAGGATA+CGG 0.583806 4.2:-89159085 MS.gene79483:CDS
GCCAAGGCAAGAATGGGACT+CGG 0.585483 4.2:-89159169 MS.gene79483:CDS
TCAATTGTGCATAGACTCCA+AGG 0.586697 4.2:+89159451 None:intergenic
CATAGACTCCAAGGATCTGA+AGG 0.587303 4.2:+89159460 None:intergenic
AAGATAACCCTGATAACCAA+AGG 0.589458 4.2:-89160023 MS.gene79483:CDS
TCCAATGGACATAAGAGCAA+GGG 0.589995 4.2:+89160098 None:intergenic
TCTGTTCTTATAAGCCGCAA+CGG 0.596903 4.2:+89159777 None:intergenic
CGAAGAAGAGCAATCAGTGA+AGG 0.596906 4.2:-89159091 MS.gene79483:CDS
AATAAGAGGCATGAAATTGG+TGG 0.602647 4.2:+89161130 None:intergenic
GTTGCAGGAATGCAAGGAGG+AGG 0.607647 4.2:+89160171 None:intergenic
CGAAACTTCGAAGTTCCAGC+CGG 0.609248 4.2:-89159301 MS.gene79483:CDS
AACAGGGAATGATACTCTTG+TGG 0.610637 4.2:-89160221 MS.gene79483:intron
TTCCAATGGACATAAGAGCA+AGG 0.616534 4.2:+89160097 None:intergenic
TCGACTACCACCTAAGAACT+CGG 0.619351 4.2:-89159749 MS.gene79483:CDS
ATTCCACCCAAGCAAAGTTG+AGG 0.630367 4.2:+89159016 None:intergenic
ATTCCAGCCGAGTTCTTAGG+TGG 0.637012 4.2:+89159742 None:intergenic
ATTACACTAAGTGAACCGGC+TGG 0.645410 4.2:+89159286 None:intergenic
ATCTGAAGGTGATCCATCAG+AGG 0.647498 4.2:+89159474 None:intergenic
GAAAGGGTAACCTCGAGAGG+TGG 0.651879 4.2:-89158851 MS.gene79483:CDS
ATGCTCCATAGCGTTTGGCG+CGG 0.653136 4.2:-89160061 MS.gene79483:CDS
GTCGAAAGGGTAACCTCGAG+AGG 0.656180 4.2:-89158854 MS.gene79483:CDS
CTTTGTACGAGTGACCGTTG+CGG 0.660158 4.2:-89159791 MS.gene79483:CDS
GATATCGGATAATATTAACA+AGG 0.662248 4.2:-89158816 MS.gene79483:CDS
CCTCTAGCCTCGAAGCTCAG+AGG 0.662495 4.2:-89159211 MS.gene79483:CDS
AAATGAGGGTTCATACGACA+AGG 0.666942 4.2:-89158642 MS.gene79483:CDS
AAGATACTTGTAGACCACAA+TGG 0.668658 4.2:+89159391 None:intergenic
ATATCGGATAATATTAACAA+GGG 0.683833 4.2:-89158815 MS.gene79483:CDS
CAATTGCTGCAGTTACCAGG+TGG 0.683991 4.2:+89159128 None:intergenic
TCAGTTGCAGGAATGCAAGG+AGG 0.690858 4.2:+89160168 None:intergenic
CAACCTCAATTACACAACAA+GGG 0.698645 4.2:-89161484 MS.gene79483:CDS
AATTATGACAGAAATAGCCG+AGG 0.702109 4.2:+89159966 None:intergenic
TTCGGACTAGGAATGGCCGT+AGG 0.705285 4.2:-89158908 MS.gene79483:CDS
CCTCAATTACACAACAAGGG+TGG 0.712528 4.2:-89161481 MS.gene79483:CDS
TGAACCTATGATGAAAGGCA+TGG 0.712733 4.2:+89161450 None:intergenic
TGGAACCTAAACCAACAGCA+AGG 0.712932 4.2:+89161072 None:intergenic
TATATTAAGACCACTCACTG+TGG 0.737154 4.2:+89158763 None:intergenic
TGTTGAACATGTCTGCAATG+TGG 0.741046 4.2:-89159045 MS.gene79483:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATATTCACAATATTTATA+TGG - chr4.2:89160935-89160954 MS.gene79483:intron 15.0%
!!! AAATATGATACTTTATTTGA+TGG - chr4.2:89158986-89159005 MS.gene79483:CDS 15.0%
!!! AATATGATACTTTATTTGAT+GGG - chr4.2:89158987-89159006 MS.gene79483:CDS 15.0%
!!! AATTTAGTGTTTTATATGTT+TGG - chr4.2:89158880-89158899 MS.gene79483:CDS 15.0%
!!! ATCAAATAAAGTATCATATT+TGG + chr4.2:89158987-89159006 None:intergenic 15.0%
!! AATATAACATGAAAGTTTAG+AGG - chr4.2:89159540-89159559 MS.gene79483:intron 20.0%
!! ACAAAATCATTTACATTGTT+TGG + chr4.2:89159922-89159941 None:intergenic 20.0%
!! ATATCGGATAATATTAACAA+GGG - chr4.2:89161381-89161400 MS.gene79483:intron 20.0%
!! CCAAATATTAAATAAGTATC+TGG + chr4.2:89159660-89159679 None:intergenic 20.0%
!! CCAGATACTTATTTAATATT+TGG - chr4.2:89159657-89159676 MS.gene79483:intron 20.0%
!! GTATTAATATCAGTAATTTG+TGG - chr4.2:89160634-89160653 MS.gene79483:intron 20.0%
!! TTATTAAAGTTATTCCATTG+TGG - chr4.2:89160791-89160810 MS.gene79483:intron 20.0%
!!! ATATGATACTTTATTTGATG+GGG - chr4.2:89158988-89159007 MS.gene79483:CDS 20.0%
!!! GTTTTATTCTTTACATAAAG+TGG + chr4.2:89160364-89160383 None:intergenic 20.0%
!!! TATTTTAGTAAGTTACTACT+AGG - chr4.2:89159743-89159762 MS.gene79483:CDS 20.0%
! AATAATAGAGTTTAGTGATC+CGG + chr4.2:89158860-89158879 None:intergenic 25.0%
! AGAAGATATAATGTATCCTA+CGG + chr4.2:89161307-89161326 None:intergenic 25.0%
! CAAATAAAGATGATGCATTT+AGG - chr4.2:89159620-89159639 MS.gene79483:intron 25.0%
! GATATCGGATAATATTAACA+AGG - chr4.2:89161380-89161399 MS.gene79483:intron 25.0%
! GTATTATGTAGTATGTAGTT+GGG - chr4.2:89161458-89161477 MS.gene79483:CDS 25.0%
! TAAAGAGCTTCTTTAATAAC+AGG - chr4.2:89159686-89159705 MS.gene79483:intron 25.0%
! TGAATATCAACTTCTCTAAA+AGG + chr4.2:89160568-89160587 None:intergenic 25.0%
! TGTATTATGTAGTATGTAGT+TGG - chr4.2:89161457-89161476 MS.gene79483:CDS 25.0%
! TTCAATGAATCTTTCTTGTT+TGG - chr4.2:89159418-89159437 MS.gene79483:CDS 25.0%
!! ATTAATTGACTTCAGAAATG+AGG - chr4.2:89161539-89161558 MS.gene79483:CDS 25.0%
!! TTAATTGACTTCAGAAATGA+GGG - chr4.2:89161540-89161559 MS.gene79483:CDS 25.0%
!!! ACACTAGACATTTTAGAAAA+TGG - chr4.2:89159277-89159296 MS.gene79483:CDS 25.0%
!!! TCATTTCTGAAGTCAATTAA+TGG + chr4.2:89161540-89161559 None:intergenic 25.0%
!!! TTCAACCTTATTTTATCAGA+TGG + chr4.2:89160514-89160533 None:intergenic 25.0%
AAAATGTCTAGTGTTTGTGT+CGG + chr4.2:89159271-89159290 None:intergenic 30.0%
AGCAAGAAAAAAGAAGAAAG+TGG + chr4.2:89160338-89160357 None:intergenic 30.0%
ATCAAAAGTAAGTGAAGATG+AGG - chr4.2:89161506-89161525 MS.gene79483:CDS 30.0%
CACACAATGACAACAAAAAT+CGG + chr4.2:89160599-89160618 None:intergenic 30.0%
CATTGGAAATATTCTTCAGT+TGG - chr4.2:89160197-89160216 MS.gene79483:CDS 30.0%
CATTGTGAAGACAAGATTAA+TGG - chr4.2:89159379-89159398 MS.gene79483:CDS 30.0%
CTAATATTGTGAGATTTCGA+CGG - chr4.2:89159476-89159495 MS.gene79483:CDS 30.0%
CTGTTCCATCTGATAAAATA+AGG - chr4.2:89160506-89160525 MS.gene79483:intron 30.0%
GAAATCTCACAATATTAGAG+AGG + chr4.2:89159474-89159493 None:intergenic 30.0%
GTATATATCCAAGATCATCT+TGG - chr4.2:89160268-89160287 MS.gene79483:intron 30.0%
TAATATTGTGAGATTTCGAC+GGG - chr4.2:89159477-89159496 MS.gene79483:CDS 30.0%
TGTCTTCACAATGAAACAAT+TGG + chr4.2:89159372-89159391 None:intergenic 30.0%
TTGGATATGTTAATGCTAAG+TGG - chr4.2:89158899-89158918 MS.gene79483:CDS 30.0%
TTTATTCAGCATTGTCGAAA+GGG - chr4.2:89161329-89161348 MS.gene79483:intron 30.0%
! ACATTGTTTGGTTCAAATGA+GGG + chr4.2:89159910-89159929 None:intergenic 30.0%
! ATCTTGATGTCACTTAACAT+TGG - chr4.2:89160823-89160842 MS.gene79483:intron 30.0%
! CAACTCAATTTTCTTCCTTT+TGG - chr4.2:89159951-89159970 MS.gene79483:CDS 30.0%
! TACATTGTTTGGTTCAAATG+AGG + chr4.2:89159911-89159930 None:intergenic 30.0%
! TTTTATTCAGCATTGTCGAA+AGG - chr4.2:89161328-89161347 MS.gene79483:intron 30.0%
! TTTTGATCAATCATAACTGC+CGG - chr4.2:89158838-89158857 MS.gene79483:CDS 30.0%
!! AAATGATAGTGTCTTTTTGC+TGG + chr4.2:89158690-89158709 None:intergenic 30.0%
!! AATGATAGTGTCTTTTTGCT+GGG + chr4.2:89158689-89158708 None:intergenic 30.0%
!! ATGTGAATGATTAACTTGTG+TGG - chr4.2:89159197-89159216 MS.gene79483:CDS 30.0%
!! ATTTTCTTCCTTTTGGTAAC+AGG - chr4.2:89159958-89159977 MS.gene79483:CDS 30.0%
!! TTTTCTTCCTTTTGGTAACA+GGG - chr4.2:89159959-89159978 MS.gene79483:CDS 30.0%
AAGATAACCCTGATAACCAA+AGG - chr4.2:89160173-89160192 MS.gene79483:CDS 35.0%
AAGATACTTGTAGACCACAA+TGG + chr4.2:89160808-89160827 None:intergenic 35.0%
AATTATGACAGAAATAGCCG+AGG + chr4.2:89160233-89160252 None:intergenic 35.0%
ACAACCTCAATTACACAACA+AGG - chr4.2:89158711-89158730 MS.gene79483:CDS 35.0%
ACCAATTTCATGCCTCTTAT+TGG - chr4.2:89159068-89159087 MS.gene79483:CDS 35.0%
ACTTACCATGAAAGTGATGA+TGG + chr4.2:89159147-89159166 None:intergenic 35.0%
AGAAGTGAACCTATGATGAA+AGG + chr4.2:89158754-89158773 None:intergenic 35.0%
AGAATATTTCCAATGCCCTT+TGG + chr4.2:89160192-89160211 None:intergenic 35.0%
AGATAACCCTGATAACCAAA+GGG - chr4.2:89160174-89160193 MS.gene79483:CDS 35.0%
ATATCCAAGATCATCTTGGA+TGG - chr4.2:89160272-89160291 MS.gene79483:intron 35.0%
ATATTGACACCAACACGATA+CGG + chr4.2:89159317-89159336 None:intergenic 35.0%
ATCTGCAACAACTTCACAAA+TGG - chr4.2:89160060-89160079 MS.gene79483:CDS 35.0%
CAACCTCAATTACACAACAA+GGG - chr4.2:89158712-89158731 MS.gene79483:CDS 35.0%
CATGATTACACTAAGTGAAC+CGG + chr4.2:89160917-89160936 None:intergenic 35.0%
GAAAAAGACTTAACCTCTGA+TGG - chr4.2:89160709-89160728 MS.gene79483:intron 35.0%
GTGAAGACAAGATTAATGGA+GGG - chr4.2:89159383-89159402 MS.gene79483:CDS 35.0%
TATATTAAGACCACTCACTG+TGG + chr4.2:89161436-89161455 None:intergenic 35.0%
TATCATTCCCTGTTACCAAA+AGG + chr4.2:89159969-89159988 None:intergenic 35.0%
TCTGTCATAATTGCATTCAC+AGG - chr4.2:89160241-89160260 MS.gene79483:intron 35.0%
TGAAGACAAGATTAATGGAG+GGG - chr4.2:89159384-89159403 MS.gene79483:CDS 35.0%
TGTGAAGACAAGATTAATGG+AGG - chr4.2:89159382-89159401 MS.gene79483:CDS 35.0%
TTTGATTCTACATCAGCAGT+AGG + chr4.2:89161492-89161511 None:intergenic 35.0%
! AATAAGAGGCATGAAATTGG+TGG + chr4.2:89159069-89159088 None:intergenic 35.0%
! ACAAATAGCACCTTGTTTAC+CGG - chr4.2:89160382-89160401 MS.gene79483:intron 35.0%
! ACCAATAAGAGGCATGAAAT+TGG + chr4.2:89159072-89159091 None:intergenic 35.0%
! CATCTTTATTTGATTCACCG+AGG + chr4.2:89159612-89159631 None:intergenic 35.0%
! CTTTTTCCTCAACTTTGCTT+GGG - chr4.2:89161174-89161193 MS.gene79483:CDS 35.0%
! GAAGTCAATTTTGTATGAGG+AGG + chr4.2:89158667-89158686 None:intergenic 35.0%
! GCTGAAGCATTCAATGTTAT+AGG - chr4.2:89161204-89161223 MS.gene79483:CDS 35.0%
! GGTTATCTTTTCTATTCACC+TGG + chr4.2:89160162-89160181 None:intergenic 35.0%
! TCAGAGGTTAAGTCTTTTTC+TGG + chr4.2:89160709-89160728 None:intergenic 35.0%
! TGACATAGTCTTGGGAAATT+CGG + chr4.2:89161250-89161269 None:intergenic 35.0%
! TGGGAAGTCAATTTTGTATG+AGG + chr4.2:89158670-89158689 None:intergenic 35.0%
! TTGATGTCACTTAACATTGG+AGG - chr4.2:89160826-89160845 MS.gene79483:intron 35.0%
!! GATATGCATTTTTCAGTTGC+AGG + chr4.2:89160043-89160062 None:intergenic 35.0%
!! TGGAAATGATGGATGGATAT+CGG - chr4.2:89161365-89161384 MS.gene79483:intron 35.0%
!! TTCATTGCTGATTCTCATCT+TGG - chr4.2:89159095-89159114 MS.gene79483:CDS 35.0%
!!! GTGGTAATGCTTTGTTTTCT+AGG - chr4.2:89160653-89160672 MS.gene79483:intron 35.0%
AAATGAGGGTTCATACGACA+AGG - chr4.2:89161554-89161573 MS.gene79483:CDS 40.0%
AACAGGGAATGATACTCTTG+TGG - chr4.2:89159975-89159994 MS.gene79483:CDS 40.0%
AGAGCAATCAGTGAAGGATA+CGG - chr4.2:89161111-89161130 MS.gene79483:CDS 40.0%
AGGTTCCATCATCACTTTCA+TGG - chr4.2:89159139-89159158 MS.gene79483:CDS 40.0%
ATCACCATGCCTTTCATCAT+AGG - chr4.2:89158742-89158761 MS.gene79483:CDS 40.0%
ATTAGTGCCAAGGCAAGAAT+GGG - chr4.2:89161021-89161040 MS.gene79483:intron 40.0%
CAGCAATTGTCGAGACTATA+AGG - chr4.2:89161082-89161101 MS.gene79483:CDS 40.0%
CTTAACATTGGAGGCTCATT+TGG - chr4.2:89160835-89160854 MS.gene79483:intron 40.0%
CTTATGTCCATTGGAAATGG+TGG - chr4.2:89160106-89160125 MS.gene79483:CDS 40.0%
CTTGCTAGAGTTGCAACATT+AGG - chr4.2:89158954-89158973 MS.gene79483:CDS 40.0%
GACATGTTCAACACTGAATG+AGG + chr4.2:89161144-89161163 None:intergenic 40.0%
GATCATCTTGGATGGAAACT+TGG - chr4.2:89160280-89160299 MS.gene79483:intron 40.0%
GCCAATAGTACTTGTCGAAT+CGG + chr4.2:89161407-89161426 None:intergenic 40.0%
GCTCTTATGTCCATTGGAAA+TGG - chr4.2:89160103-89160122 MS.gene79483:CDS 40.0%
GTAGTATGTAGTTGGGCTTA+TGG - chr4.2:89161465-89161484 MS.gene79483:CDS 40.0%
GTGTGGAAGAAGAGATGAAT+AGG - chr4.2:89159214-89159233 MS.gene79483:CDS 40.0%
GTTTCCATCCAAGATGATCT+TGG + chr4.2:89160279-89160298 None:intergenic 40.0%
TCAATTGTGCATAGACTCCA+AGG + chr4.2:89160748-89160767 None:intergenic 40.0%
TCCAATGGACATAAGAGCAA+GGG + chr4.2:89160101-89160120 None:intergenic 40.0%
TCTAGTGAAATCTCTCTCCT+CGG - chr4.2:89159592-89159611 MS.gene79483:intron 40.0%
TCTGTTCTTATAAGCCGCAA+CGG + chr4.2:89160422-89160441 None:intergenic 40.0%
TGAACCTATGATGAAAGGCA+TGG + chr4.2:89158749-89158768 None:intergenic 40.0%
TGGTGGTTGCAACAGATAAA+AGG + chr4.2:89159052-89159071 None:intergenic 40.0%
TGTTGAACATGTCTGCAATG+TGG - chr4.2:89161151-89161170 MS.gene79483:CDS 40.0%
TTCCAATGGACATAAGAGCA+AGG + chr4.2:89160102-89160121 None:intergenic 40.0%
! CGGTGTAGTAGAACTCATTT+TGG + chr4.2:89161230-89161249 None:intergenic 40.0%
! CTTGGATGGAAACTTGGTTT+TGG - chr4.2:89160286-89160305 MS.gene79483:intron 40.0%
! GAAAAGAACATAAGTGCTGC+TGG + chr4.2:89160316-89160335 None:intergenic 40.0%
! GTTTGGCTCAACGATTTGAA+GGG - chr4.2:89159435-89159454 MS.gene79483:CDS 40.0%
! GTTTGGTTCAAATGAGGGAA+GGG + chr4.2:89159905-89159924 None:intergenic 40.0%
! GTTTTTCTCTTTTCTCCACC+TGG - chr4.2:89161053-89161072 MS.gene79483:intron 40.0%
! TGTTTGGCTCAACGATTTGA+AGG - chr4.2:89159434-89159453 MS.gene79483:CDS 40.0%
! TGTTTGGTTCAAATGAGGGA+AGG + chr4.2:89159906-89159925 None:intergenic 40.0%
! TTTGGCTCAACGATTTGAAG+GGG - chr4.2:89159436-89159455 MS.gene79483:CDS 40.0%
! TTTGGTTCAAATGAGGGAAG+GGG + chr4.2:89159904-89159923 None:intergenic 40.0%
! TTTTCAGTTGCAGGAATGCA+AGG + chr4.2:89160034-89160053 None:intergenic 40.0%
!! AGCATCGCTTCATCACTTTT+CGG - chr4.2:89161270-89161289 MS.gene79483:intron 40.0%
!! ATCACTTTTCGGACTAGGAA+TGG - chr4.2:89161281-89161300 MS.gene79483:intron 40.0%
!! GCAATGAAAGCACCAATAAG+AGG + chr4.2:89159083-89159102 None:intergenic 40.0%
!! GCTTTTTCCTCAACTTTGCT+TGG - chr4.2:89161173-89161192 MS.gene79483:CDS 40.0%
!! GTATCGTGTTGGTGTCAATA+TGG - chr4.2:89159316-89159335 MS.gene79483:CDS 40.0%
!! TTGCTGATTCTCATCTTGGA+CGG - chr4.2:89159099-89159118 MS.gene79483:CDS 40.0%
!! TTGGTGTCAATATGGAGTGT+CGG - chr4.2:89159324-89159343 MS.gene79483:CDS 40.0%
AGAGATTTCACTAGAGGAGG+AGG + chr4.2:89159587-89159606 None:intergenic 45.0%
AGCGATGCTTGACATAGTCT+TGG + chr4.2:89161259-89161278 None:intergenic 45.0%
ATCGAATGTCCGTATCGTGT+TGG - chr4.2:89159305-89159324 MS.gene79483:CDS 45.0%
ATCTGAAGGTGATCCATCAG+AGG + chr4.2:89160725-89160744 None:intergenic 45.0%
ATGGATCACCTTCAGATCCT+TGG - chr4.2:89160728-89160747 MS.gene79483:intron 45.0%
ATTACACTAAGTGAACCGGC+TGG + chr4.2:89160913-89160932 None:intergenic 45.0%
ATTCCACCCAAGCAAAGTTG+AGG + chr4.2:89161183-89161202 None:intergenic 45.0%
CATAGACTCCAAGGATCTGA+AGG + chr4.2:89160739-89160758 None:intergenic 45.0%
CCAATGCCCTTTGGTTATCA+GGG + chr4.2:89160183-89160202 None:intergenic 45.0%
CCTCAATTACACAACAAGGG+TGG - chr4.2:89158715-89158734 MS.gene79483:CDS 45.0%
CGAAGAAGAGCAATCAGTGA+AGG - chr4.2:89161105-89161124 MS.gene79483:CDS 45.0%
CTTCAATGCTCCATAGCGTT+TGG - chr4.2:89160130-89160149 MS.gene79483:CDS 45.0%
GACAAGATTAATGGAGGGGA+TGG - chr4.2:89159388-89159407 MS.gene79483:CDS 45.0%
GAGAGAGATTTCACTAGAGG+AGG + chr4.2:89159590-89159609 None:intergenic 45.0%
GAGGAGAGAGATTTCACTAG+AGG + chr4.2:89159593-89159612 None:intergenic 45.0%
GATTAGTGCCAAGGCAAGAA+TGG - chr4.2:89161020-89161039 MS.gene79483:intron 45.0%
GCGATGCTTGACATAGTCTT+GGG + chr4.2:89161258-89161277 None:intergenic 45.0%
TACATTCCAGCCGAGTTCTT+AGG + chr4.2:89160460-89160479 None:intergenic 45.0%
TCCAATGCCCTTTGGTTATC+AGG + chr4.2:89160184-89160203 None:intergenic 45.0%
TCCCTTGCTCTTATGTCCAT+TGG - chr4.2:89160097-89160116 MS.gene79483:CDS 45.0%
TCGACTACCACCTAAGAACT+CGG - chr4.2:89160447-89160466 MS.gene79483:intron 45.0%
TCGTACAAAGCCGGTAAACA+AGG + chr4.2:89160395-89160414 None:intergenic 45.0%
TGGAACCTAAACCAACAGCA+AGG + chr4.2:89159127-89159146 None:intergenic 45.0%
TTGAAGGCCACCATTTCCAA+TGG + chr4.2:89160116-89160135 None:intergenic 45.0%
TTTCCTCAACTTTGCTTGGG+TGG - chr4.2:89161177-89161196 MS.gene79483:CDS 45.0%
! CAGTTGGTACTATGCTTCCT+CGG - chr4.2:89160213-89160232 MS.gene79483:CDS 45.0%
! CCACCCTTGTTGTGTAATTG+AGG + chr4.2:89158718-89158737 None:intergenic 45.0%
! GGTGTTACATAGCTCATCTG+TGG - chr4.2:89159345-89159364 MS.gene79483:CDS 45.0%
!! CAAACGCTATGGAGCATTGA+AGG + chr4.2:89160132-89160151 None:intergenic 45.0%
!! GCCGATTCGACAAGTACTAT+TGG - chr4.2:89161403-89161422 MS.gene79483:intron 45.0%
!! GCTTCATCACTTTTCGGACT+AGG - chr4.2:89161276-89161295 MS.gene79483:intron 45.0%
!!! GGAGATGATTTTGAGGTGCT+TGG + chr4.2:89159883-89159902 None:intergenic 45.0%
AACGGTCACTCGTACAAAGC+CGG + chr4.2:89160404-89160423 None:intergenic 50.0%
AAGGAGGAGGTCTTGCTTGT+GGG + chr4.2:89160015-89160034 None:intergenic 50.0%
ACCTCGAGAGGTGGAAATGA+TGG - chr4.2:89161354-89161373 MS.gene79483:intron 50.0%
AGGTCTTGCTTGTGGGATCA+TGG + chr4.2:89160008-89160027 None:intergenic 50.0%
ATTCCAGCCGAGTTCTTAGG+TGG + chr4.2:89160457-89160476 None:intergenic 50.0%
CAATTGCTGCAGTTACCAGG+TGG + chr4.2:89161071-89161090 None:intergenic 50.0%
CGAAACTTCGAAGTTCCAGC+CGG - chr4.2:89160895-89160914 MS.gene79483:intron 50.0%
CGACAATTGCTGCAGTTACC+AGG + chr4.2:89161074-89161093 None:intergenic 50.0%
CGGCTGGAACTTCGAAGTTT+CGG + chr4.2:89160897-89160916 None:intergenic 50.0%
CTCAACGATTTGAAGGGGCA+CGG - chr4.2:89159441-89159460 MS.gene79483:CDS 50.0%
CTCGAAGCTCAGAGGAAAAC+CGG - chr4.2:89160993-89161012 MS.gene79483:intron 50.0%
CTTTGTACGAGTGACCGTTG+CGG - chr4.2:89160405-89160424 MS.gene79483:intron 50.0%
GATTTCACTAGAGGAGGAGG+AGG + chr4.2:89159584-89159603 None:intergenic 50.0%
GGCTGGAACTTCGAAGTTTC+GGG + chr4.2:89160896-89160915 None:intergenic 50.0%
TCAGTTGCAGGAATGCAAGG+AGG + chr4.2:89160031-89160050 None:intergenic 50.0%
TCCATCATTTCCACCTCTCG+AGG + chr4.2:89161358-89161377 None:intergenic 50.0%
TGGCTTATTGCCACAGTGAG+TGG - chr4.2:89161423-89161442 MS.gene79483:intron 50.0%
TTGTGAGATTTCGACGGGAG+AGG - chr4.2:89159482-89159501 MS.gene79483:CDS 50.0%
! CCCTGATAACCAAAGGGCAT+TGG - chr4.2:89160180-89160199 MS.gene79483:CDS 50.0%
! CGAGAGGTGGAAATGATGGA+TGG - chr4.2:89161358-89161377 MS.gene79483:intron 50.0%
! GGGAAGGGGAGATGATTTTG+AGG + chr4.2:89159890-89159909 None:intergenic 50.0%
!! CGGTTCCTTGCTGTTGGTTT+AGG - chr4.2:89159119-89159138 MS.gene79483:CDS 50.0%
AGCTCAGAGGAAAACCGGTC+AGG - chr4.2:89160998-89161017 MS.gene79483:intron 55.0%
ATGCTCCATAGCGTTTGGCG+CGG - chr4.2:89160135-89160154 MS.gene79483:CDS 55.0%
CAAGGAGGAGGTCTTGCTTG+TGG + chr4.2:89160016-89160035 None:intergenic 55.0%
CTACCACCTAAGAACTCGGC+TGG - chr4.2:89160451-89160470 MS.gene79483:intron 55.0%
GAAAGGGTAACCTCGAGAGG+TGG - chr4.2:89161345-89161364 MS.gene79483:intron 55.0%
GCCAAGGCAAGAATGGGACT+CGG - chr4.2:89161027-89161046 MS.gene79483:intron 55.0%
GCCTTGGCACTAATCCTGAC+CGG + chr4.2:89161015-89161034 None:intergenic 55.0%
GTCGAAAGGGTAACCTCGAG+AGG - chr4.2:89161342-89161361 MS.gene79483:intron 55.0%
GTTGCAGGAATGCAAGGAGG+AGG + chr4.2:89160028-89160047 None:intergenic 55.0%
TCCGAGTCCCATTCTTGCCT+TGG + chr4.2:89161031-89161050 None:intergenic 55.0%
! ACCGGTCAGGATTAGTGCCA+AGG - chr4.2:89161011-89161030 MS.gene79483:intron 55.0%
! CGGTTTTCCTCTGAGCTTCG+AGG + chr4.2:89160995-89161014 None:intergenic 55.0%
! CTTGGACGGTTCCTTGCTGT+TGG - chr4.2:89159113-89159132 MS.gene79483:CDS 55.0%
! TTCGGACTAGGAATGGCCGT+AGG - chr4.2:89161288-89161307 MS.gene79483:intron 55.0%
CCTCTAGCCTCGAAGCTCAG+AGG - chr4.2:89160985-89161004 MS.gene79483:intron 60.0%
CCTCTGAGCTTCGAGGCTAG+AGG + chr4.2:89160988-89161007 None:intergenic 60.0%
CATAGCGTTTGGCGCGGACC+AGG - chr4.2:89160141-89160160 MS.gene79483:CDS 65.0%
CTGGTCCGCGCCAAACGCTA+TGG + chr4.2:89160143-89160162 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 89158634 89161584 89158634 ID=MS.gene79483
chr4.2 mRNA 89158634 89161584 89158634 ID=MS.gene79483.t1;Parent=MS.gene79483
chr4.2 exon 89161455 89161584 89161455 ID=MS.gene79483.t1.exon1;Parent=MS.gene79483.t1
chr4.2 CDS 89161455 89161584 89161455 ID=cds.MS.gene79483.t1;Parent=MS.gene79483.t1
chr4.2 exon 89161058 89161275 89161058 ID=MS.gene79483.t1.exon2;Parent=MS.gene79483.t1
chr4.2 CDS 89161058 89161275 89161058 ID=cds.MS.gene79483.t1;Parent=MS.gene79483.t1
chr4.2 exon 89159691 89160238 89159691 ID=MS.gene79483.t1.exon3;Parent=MS.gene79483.t1
chr4.2 CDS 89159691 89160238 89159691 ID=cds.MS.gene79483.t1;Parent=MS.gene79483.t1
chr4.2 exon 89158634 89159543 89158634 ID=MS.gene79483.t1.exon4;Parent=MS.gene79483.t1
chr4.2 CDS 89158634 89159543 89158634 ID=cds.MS.gene79483.t1;Parent=MS.gene79483.t1
Gene Sequence

>MS.gene79483

ATGGAGAAGAATGAAATAGAGCTTTCTTCACCTCCTCATACAAAATTGACTTCCCAGCAAAAAGACACTATCATTTCACAACCTCAATTACACAACAAGGGTGGCTTCATCACCATGCCTTTCATCATAGGTTCACTTCTTTTTATGTTGAAATTAATTTATTGATATTCATTTAATTTCAATGTAAAGAAACTTTCTTTACACTTTTGATCAATCATAACTGCCGGATCACTAAACTCTATTATTAATTTAGTGTTTTATATGTTTGGATATGTTAATGCTAAGTGGTAATTAATGTGTGAATTGCAGCAAATGAATCACTTGCTAGAGTTGCAACATTAGGATTATTGCCAAATATGATACTTTATTTGATGGGGAGTTATAATCTTCATTTATCTAAAGCTACTCATATACTCCTTTTATCTGTTGCAACCACCAATTTCATGCCTCTTATTGGTGCTTTCATTGCTGATTCTCATCTTGGACGGTTCCTTGCTGTTGGTTTAGGTTCCATCATCACTTTCATGGTAAGTAGACTACTTTTCATTTTTTCTATCATTATCATGTGAATGATTAACTTGTGTGGAAGAAGAGATGAATAGGATATGTATTTTGACTCATTTGCTAAAAAGCACCGACACAAACACTAGACATTTTAGAAAATGGAATTGATCGAATGTCCGTATCGTGTTGGTGTCAATATGGAGTGTCGGTGTTACATAGCTCATCTGTGGACCAATTGTTTCATTGTGAAGACAAGATTAATGGAGGGGATGGAAAGATTTTCAATGAATCTTTCTTGTTTGGCTCAACGATTTGAAGGGGCACGGCATCTATCCTCTCTAATATTGTGAGATTTCGACGGGAGAGGATACGTGTTTAAGATATTTTAAACATGTTGACTTTAATATAACATGAAAGTTTAGAGGTTGTAAAATGTAATTAGTCCTCCTCCTCCTCTAGTGAAATCTCTCTCCTCGGTGAATCAAATAAAGATGATGCATTTAGGTGTGAAGAGCTTAACCAGATACTTATTTAATATTTGGCAATCTTAAAGAGCTTCTTTAATAACAGGTTATAGTAGAACTCTGCATAAGCTATGTATAAACTATTTTAGTAAGTTACTACTAGGAGCTTATGAAATAAGCTTAAAATAGTTTATGAATACTTCATACGTTATGTTATAAACTCGTTCAAGCTCTTACATAAATGCTTACGTCTAAAGTTAAGCACAAAATAAGCTCTTCCAAGCACCTCAAAATCATCTCCCCTTCCCTCATTTGAACCAAACAATGTAAATGATTTTGTATTGAAAAACAACTCAATTTTCTTCCTTTTGGTAACAGGGAATGATACTCTTGTGGCTAACAGCCATGATCCCACAAGCAAGACCTCCTCCTTGCATTCCTGCAACTGAAAAATGCATATCTGCAACAACTTCACAAATGGCAATGCTGATTTCTTCCCTTGCTCTTATGTCCATTGGAAATGGTGGCCTTCAATGCTCCATAGCGTTTGGCGCGGACCAGGTGAATAGAAAAGATAACCCTGATAACCAAAGGGCATTGGAAATATTCTTCAGTTGGTACTATGCTTCCTCGGCTATTTCTGTCATAATTGCATTCACAGGAATAGTATATATCCAAGATCATCTTGGATGGAAACTTGGTTTTGGTGTTCCAGCAGCACTTATGTTCTTTTCCACTTTCTTCTTTTTTCTTGCTTCTCCACTTTATGTAAAGAATAAAACAAATAGCACCTTGTTTACCGGCTTTGTACGAGTGACCGTTGCGGCTTATAAGAACAGAAACATTCGACTACCACCTAAGAACTCGGCTGGAATGTATCATCATATGAAGAATTCTGAACTTACTGTTCCATCTGATAAAATAAGGTTGAATCACTTACATATTCACATTAGTATTAGAGTTCCTTTTAGAGAAGTTGATATTCAAATTATTGCCGATTTTTGTTGTCATTGTGTGTTAAAATATCATTATGTATTAATATCAGTAATTTGTGGTAATGCTTTGTTTTCTAGGTTTCTGAATAAAGCTTGCTACATTAGAAGTCCAGAAAAAGACTTAACCTCTGATGGATCACCTTCAGATCCTTGGAGTCTATGCACAATTGATCAAGTAGAAGAATTAAAAGCTATTATTAAAGTTATTCCATTGTGGTCTACAAGTATCTTGATGTCACTTAACATTGGAGGCTCATTTGGATTACTGCAAGCGAAATCGCTGAACAGACACATCACCCGAAACTTCGAAGTTCCAGCCGGTTCACTTAGTGTAATCATGATATTCACAATATTTATATGGATAGCTCTTTATGATCGTGTCGTTATTCCTCTAGCCTCGAAGCTCAGAGGAAAACCGGTCAGGATTAGTGCCAAGGCAAGAATGGGACTCGGATTGTTTTTCTCTTTTCTCCACCTGGTAACTGCAGCAATTGTCGAGACTATAAGGCGAAGAAGAGCAATCAGTGAAGGATACGGTAATGATCCTCATTCAGTGTTGAACATGTCTGCAATGTGGCTTTTTCCTCAACTTTGCTTGGGTGGAATAGCTGAAGCATTCAATGTTATAGGCCAAAATGAGTTCTACTACACCGAATTTCCCAAGACTATGTCAAGCATCGCTTCATCACTTTTCGGACTAGGAATGGCCGTAGGATACATTATATCTTCTTTTTTATTCAGCATTGTCGAAAGGGTAACCTCGAGAGGTGGAAATGATGGATGGATATCGGATAATATTAACAAGGGCCGATTCGACAAGTACTATTGGCTTATTGCCACAGTGAGTGGTCTTAATATACTGTATTATGTAGTATGTAGTTGGGCTTATGGACCTACTGCTGATGTAGAATCAAAAGTAAGTGAAGATGAGGAAGAATTACCATTAATTGACTTCAGAAATGAGGGTTCATACGACAAGGATAACTGA
Protein sequence

>MS.gene79483.t1

MEKNEIELSSPPHTKLTSQQKDTIISQPQLHNKGGFITMPFIIANESLARVATLGLLPNMILYLMGSYNLHLSKATHILLLSVATTNFMPLIGAFIADSHLGRFLAVGLGSIITFMGMILLWLTAMIPQARPPPCIPATEKCISATTSQMAMLISSLALMSIGNGGLQCSIAFGADQVNRKDNPDNQRALEIFFSWYYASSAISVIIAFTGIVYIQDHLGWKLGFGVPAALMFFSTFFFFLASPLYVKNKTNSTLFTGFVRVTVAAYKNRNIRLPPKNSAGMYHHMKNSELTVPSDKIRFLNKACYIRSPEKDLTSDGSPSDPWSLCTIDQVEELKAIIKVIPLWSTSILMSLNIGGSFGLLQAKSLNRHITRNFEVPAGSLSVIMIFTIFIWIALYDRVVIPLASKLRGKPVRISAKARMGLGLFFSFLHLVTAAIVETIRRRRAISEGYGNDPHSVLNMSAMWLFPQLCLGGIAEAFNVIGQNEFYYTEFPKTMSSIASSLFGLGMAVGYIISSFLFSIVERVTSRGGNDGWISDNINKGRFDKYYWLIATVSGLNILYYVVCSWAYGPTADVESKVSEDEEELPLIDFRNEGSYDKDN