AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene38100


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38100.t1 MTR_5g097970 96.296 162 5 1 1 161 1 162 6.15e-113 317
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene38100.t1 AT2G04790 62.069 145 52 2 15 156 36 180 6.27e-65 197
MS.gene38100.t1 AT2G04790 62.069 145 52 2 15 156 34 178 7.90e-65 196
MS.gene38100.t1 AT2G04790 62.069 145 52 2 15 156 17 161 1.01e-64 195
MS.gene38100.t1 AT2G04790 65.152 132 43 2 15 143 36 167 1.06e-61 188
MS.gene38100.t1 AT2G04790 65.152 132 43 2 15 143 34 165 1.15e-61 188
MS.gene38100.t1 AT2G04790 65.152 132 43 2 15 143 17 148 1.86e-61 187
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 36 112 6.00e-32 111
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 36 112 6.00e-32 111
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 17 93 1.00e-31 109
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 17 93 1.00e-31 109
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 36 112 1.49e-31 110
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 36 112 1.49e-31 110
MS.gene38100.t1 AT2G04790 64.935 77 25 1 15 89 17 93 2.22e-31 109
MS.gene38100.t1 AT2G04790 72.727 44 12 0 15 58 36 79 3.04e-18 75.1
MS.gene38100.t1 AT2G04790 72.727 44 12 0 15 58 36 79 3.04e-18 75.1
MS.gene38100.t1 AT2G04790 72.727 44 12 0 15 58 36 79 3.04e-18 75.1

Find 38 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTGCTTACTCCCTTAATATT+TGG 0.106470 5.1:-78355351 MS.gene38100:CDS
GCTGCAAATCCCAAATATTA+AGG 0.224867 5.1:+78355341 None:intergenic
TGCTTACTCCCTTAATATTT+GGG 0.251158 5.1:-78355350 MS.gene38100:CDS
TATATATCTTCAGGATCTTT+TGG 0.303960 5.1:+78355112 None:intergenic
ATGTGGCAGCAATGCCTCTA+AGG 0.323320 5.1:-78355408 MS.gene38100:CDS
TTGGGATTTGCAGCATTTCA+TGG 0.365079 5.1:-78355332 MS.gene38100:CDS
GGTCCCTTTGAAGCCCTTAG+AGG 0.394810 5.1:+78355394 None:intergenic
CAAACCTTCCTCACCTTCAA+AGG 0.430343 5.1:-78355302 MS.gene38100:intron
CTGCAAATCCCAAATATTAA+GGG 0.434798 5.1:+78355342 None:intergenic
ACCAACCAGCATTTGCTTCG+CGG 0.449071 5.1:+78354909 None:intergenic
AATAGGATTGAAACCTTTGA+AGG 0.455552 5.1:+78355289 None:intergenic
AATGCCTCTAAGGGCTTCAA+AGG 0.472734 5.1:-78355398 MS.gene38100:CDS
AGTGCCGCATATATATCTTC+AGG 0.473960 5.1:+78355103 None:intergenic
TGGGAAACAGATTTGAGAAT+CGG 0.477510 5.1:-78354834 MS.gene38100:CDS
AGAAGAAGATGCTGTGGAAA+TGG 0.484961 5.1:-78354877 MS.gene38100:CDS
CGAAGCAAATGCTGGTTGGT+TGG 0.522860 5.1:-78354906 MS.gene38100:CDS
GGTTTGGTCAGACAACTCAT+TGG 0.526406 5.1:-78354606 MS.gene38100:CDS
GAAACCTTTGAAGGTGAGGA+AGG 0.532186 5.1:+78355298 None:intergenic
AGGCAGAGAGGAAGTGTATG+TGG 0.544412 5.1:-78355425 MS.gene38100:CDS
TGAATTACCTCTAGCTACCC+TGG 0.544685 5.1:+78354553 None:intergenic
TTATCTGGTAGAGCTGTGCC+AGG 0.545939 5.1:-78355073 MS.gene38100:intron
TCAAAGACAAGAATGGTAAG+AGG 0.550312 5.1:-78355445 MS.gene38100:CDS
GATTGCCGCGAAGCAAATGC+TGG 0.556329 5.1:-78354914 MS.gene38100:CDS
ACTTGTATCAAAGACAAGAA+TGG 0.568325 5.1:-78355452 MS.gene38100:CDS
TGTGGCAGCAATGCCTCTAA+GGG 0.570128 5.1:-78355407 MS.gene38100:CDS
GATTGTCGCGATTATACCAA+TGG 0.578050 5.1:-78354804 MS.gene38100:intron
ATGCCTCTAAGGGCTTCAAA+GGG 0.584284 5.1:-78355397 MS.gene38100:CDS
GATTGAAACCTTTGAAGGTG+AGG 0.593749 5.1:+78355294 None:intergenic
GCTGAAACGTTTGAGAAACC+AGG 0.597971 5.1:-78354571 MS.gene38100:CDS
GCAGCAGACATAAGCAATTG+TGG 0.599579 5.1:+78355370 None:intergenic
TCTCAGGAGCTGTTCTTGTG+AGG 0.603725 5.1:-78354947 MS.gene38100:intron
GCCGCGAAGCAAATGCTGGT+TGG 0.618375 5.1:-78354910 MS.gene38100:CDS
AAGAATGGTAAGAGGCAGAG+AGG 0.650232 5.1:-78355437 MS.gene38100:CDS
CTGAAACGTTTGAGAAACCA+GGG 0.653890 5.1:-78354570 MS.gene38100:CDS
TGAGAAACCAGGGTAGCTAG+AGG 0.673317 5.1:-78354560 None:intergenic
AGATCCTGAAGATATATATG+CGG 0.681441 5.1:-78355107 MS.gene38100:CDS
ATATGCAGAAGAAGATGCTG+TGG 0.682714 5.1:-78354883 MS.gene38100:CDS
GCAGACATAAGCAATTGTGG+TGG 0.708742 5.1:+78355373 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAAGAAAGTGAATATTTA+TGG + chr5.1:78355029-78355048 None:intergenic 15.0%
!!! TTTTCGTTAAAAAAAAGTTT+AGG - chr5.1:78354782-78354801 MS.gene38100:intron 15.0%
!! AAGTGAATTCAACAAAAAAT+GGG - chr5.1:78355170-78355189 MS.gene38100:intron 20.0%
!! TGATAATATACAAACACTTA+TGG + chr5.1:78355306-78355325 None:intergenic 20.0%
!! TTAAACAACATTATTACCAT+TGG + chr5.1:78355238-78355257 None:intergenic 20.0%
!!! TATTGCTTGTATTTATTTTC+AGG - chr5.1:78354868-78354887 MS.gene38100:CDS 20.0%
!!! TGAATTTTTGGTCAATTTAT+AGG - chr5.1:78355396-78355415 MS.gene38100:CDS 20.0%
!!! TTCAATCTGAATTGAATTTT+TGG - chr5.1:78355384-78355403 MS.gene38100:CDS 20.0%
! ATTCTGTTCATATAATTCTC+AGG - chr5.1:78355060-78355079 MS.gene38100:intron 25.0%
! CAAGTGAATTCAACAAAAAA+TGG - chr5.1:78355169-78355188 MS.gene38100:intron 25.0%
! GAGAATTATATGAACAGAAT+TGG + chr5.1:78355060-78355079 None:intergenic 25.0%
! TGCTACATAATTCTTTACAT+AGG + chr5.1:78355282-78355301 None:intergenic 25.0%
!! TATATATCTTCAGGATCTTT+TGG + chr5.1:78354914-78354933 None:intergenic 25.0%
!! TTTTGGTCAATTTATAGGTT+TGG - chr5.1:78355401-78355420 MS.gene38100:CDS 25.0%
AATAGGATTGAAACCTTTGA+AGG + chr5.1:78354737-78354756 None:intergenic 30.0%
ACGAAAAACCGATAAAATGT+AGG + chr5.1:78354769-78354788 None:intergenic 30.0%
ACTTGTATCAAAGACAAGAA+TGG - chr5.1:78354571-78354590 MS.gene38100:CDS 30.0%
AGATCCTGAAGATATATATG+CGG - chr5.1:78354916-78354935 MS.gene38100:CDS 30.0%
CTGCAAATCCCAAATATTAA+GGG + chr5.1:78354684-78354703 None:intergenic 30.0%
TGCTTACTCCCTTAATATTT+GGG - chr5.1:78354673-78354692 MS.gene38100:intron 30.0%
! ATTCCTCTCCTACATTTTAT+CGG - chr5.1:78354758-78354777 MS.gene38100:intron 30.0%
! TAACTTGTGAACTTTCTGAT+TGG - chr5.1:78354993-78355012 MS.gene38100:intron 30.0%
AAACCGATAAAATGTAGGAG+AGG + chr5.1:78354764-78354783 None:intergenic 35.0%
AATGTAGGAGAGGAATGAAT+AGG + chr5.1:78354754-78354773 None:intergenic 35.0%
AATTAAGTCCAAATCTGACC+TGG + chr5.1:78354971-78354990 None:intergenic 35.0%
CTGCTTACTCCCTTAATATT+TGG - chr5.1:78354672-78354691 MS.gene38100:intron 35.0%
GCTGCAAATCCCAAATATTA+AGG + chr5.1:78354685-78354704 None:intergenic 35.0%
TCAAAGACAAGAATGGTAAG+AGG - chr5.1:78354578-78354597 MS.gene38100:CDS 35.0%
TGGGAAACAGATTTGAGAAT+CGG - chr5.1:78355189-78355208 MS.gene38100:intron 35.0%
AGAAGAAGATGCTGTGGAAA+TGG - chr5.1:78355146-78355165 MS.gene38100:intron 40.0%
AGTGCCGCATATATATCTTC+AGG + chr5.1:78354923-78354942 None:intergenic 40.0%
ATATGCAGAAGAAGATGCTG+TGG - chr5.1:78355140-78355159 MS.gene38100:intron 40.0%
CTGAAACGTTTGAGAAACCA+GGG - chr5.1:78355453-78355472 MS.gene38100:CDS 40.0%
GATTGTCGCGATTATACCAA+TGG - chr5.1:78355219-78355238 MS.gene38100:intron 40.0%
TTGGGATTTGCAGCATTTCA+TGG - chr5.1:78354691-78354710 MS.gene38100:intron 40.0%
! GATTGAAACCTTTGAAGGTG+AGG + chr5.1:78354732-78354751 None:intergenic 40.0%
AAGAATGGTAAGAGGCAGAG+AGG - chr5.1:78354586-78354605 MS.gene38100:CDS 45.0%
CAAACCTTCCTCACCTTCAA+AGG - chr5.1:78354721-78354740 MS.gene38100:intron 45.0%
GCAGACATAAGCAATTGTGG+TGG + chr5.1:78354653-78354672 None:intergenic 45.0%
GCAGCAGACATAAGCAATTG+TGG + chr5.1:78354656-78354675 None:intergenic 45.0%
GCTGAAACGTTTGAGAAACC+AGG - chr5.1:78355452-78355471 MS.gene38100:CDS 45.0%
TTAGACGCTCTCTAAGCTTG+AGG + chr5.1:78354840-78354859 None:intergenic 45.0%
! AATGCCTCTAAGGGCTTCAA+AGG - chr5.1:78354625-78354644 MS.gene38100:intron 45.0%
! ATGCCTCTAAGGGCTTCAAA+GGG - chr5.1:78354626-78354645 MS.gene38100:intron 45.0%
! GAAACCTTTGAAGGTGAGGA+AGG + chr5.1:78354728-78354747 None:intergenic 45.0%
! GGTTTGGTCAGACAACTCAT+TGG - chr5.1:78355417-78355436 MS.gene38100:CDS 45.0%
ACCAACCAGCATTTGCTTCG+CGG + chr5.1:78355117-78355136 None:intergenic 50.0%
ATGTGGCAGCAATGCCTCTA+AGG - chr5.1:78354615-78354634 MS.gene38100:intron 50.0%
GTCCGAATGTTCACCATCGA+CGG - chr5.1:78354813-78354832 MS.gene38100:CDS 50.0%
GTCCGTCGATGGTGAACATT+CGG + chr5.1:78354818-78354837 None:intergenic 50.0%
TCTCAGGAGCTGTTCTTGTG+AGG - chr5.1:78355076-78355095 MS.gene38100:CDS 50.0%
TGTGGCAGCAATGCCTCTAA+GGG - chr5.1:78354616-78354635 MS.gene38100:intron 50.0%
TTATCTGGTAGAGCTGTGCC+AGG - chr5.1:78354950-78354969 MS.gene38100:intron 50.0%
! AGGCAGAGAGGAAGTGTATG+TGG - chr5.1:78354598-78354617 MS.gene38100:CDS 50.0%
! CGAAGCAAATGCTGGTTGGT+TGG - chr5.1:78355117-78355136 MS.gene38100:CDS 50.0%
!! GCGGCACTAGCAGTTTTATC+TGG - chr5.1:78354935-78354954 MS.gene38100:CDS 50.0%
CTAAGCTTGAGGTCCGTCGA+TGG + chr5.1:78354829-78354848 None:intergenic 55.0%
GAGCTGTGCCAGGTCAGATT+TGG - chr5.1:78354960-78354979 MS.gene38100:intron 55.0%
GATTGCCGCGAAGCAAATGC+TGG - chr5.1:78355109-78355128 MS.gene38100:CDS 55.0%
!! GGTCCCTTTGAAGCCCTTAG+AGG + chr5.1:78354632-78354651 None:intergenic 55.0%
GCCGCGAAGCAAATGCTGGT+TGG - chr5.1:78355113-78355132 MS.gene38100:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 78354563 78355482 78354563 ID=MS.gene38100
chr5.1 mRNA 78354563 78355482 78354563 ID=MS.gene38100.t1;Parent=MS.gene38100
chr5.1 exon 78355303 78355482 78355303 ID=MS.gene38100.t1.exon1;Parent=MS.gene38100.t1
chr5.1 CDS 78355303 78355482 78355303 ID=cds.MS.gene38100.t1;Parent=MS.gene38100.t1
chr5.1 exon 78355074 78355155 78355074 ID=MS.gene38100.t1.exon2;Parent=MS.gene38100.t1
chr5.1 CDS 78355074 78355155 78355074 ID=cds.MS.gene38100.t1;Parent=MS.gene38100.t1
chr5.1 exon 78354805 78354963 78354805 ID=MS.gene38100.t1.exon3;Parent=MS.gene38100.t1
chr5.1 CDS 78354805 78354963 78354805 ID=cds.MS.gene38100.t1;Parent=MS.gene38100.t1
chr5.1 exon 78354563 78354627 78354563 ID=MS.gene38100.t1.exon4;Parent=MS.gene38100.t1
chr5.1 CDS 78354563 78354627 78354563 ID=cds.MS.gene38100.t1;Parent=MS.gene38100.t1
Gene Sequence

>MS.gene38100

ATGGAATCACTTGTATCAAAGACAAGAATGGTAAGAGGCAGAGAGGAAGTGTATGTGGCAGCAATGCCTCTAAGGGCTTCAAAGGGACCACCACAATTGCTTATGTCTGCTGCTTACTCCCTTAATATTTGGGATTTGCAGCATTTCATGGTCATCATCAAACCTTCCTCACCTTCAAAGGTTTCAATCCTATTCATTCCTCTCCTACATTTTATCGGTTTTTCGTTAAAAAAAAGTTTAGGCGAGTTGAGTCCGAATGTTCACCATCGACGGACCTCAAGCTTAGAGAGCGTCTAATATATTTTTATTGCTTGTATTTATTTTCAGGTTCTAGTTTTTGATTTTCAACCAAAAGATCCTGAAGATATATATGCGGCACTAGCAGTTTTATCTGGTAGAGCTGTGCCAGGTCAGATTTGGACTTAATTCATAACTTGTGAACTTTCTGATTGGATTTTTCTCTCCATAAATATTCACTTTCTTTATATAAATCACCAATTCTGTTCATATAATTCTCAGGAGCTGTTCTTGTGAGGAAGTTAAAAAGATTGCCGCGAAGCAAATGCTGGTTGGTTGGATATGCAGAAGAAGATGCTGTGGAAATGGCAAGTGAATTCAACAAAAAATGGGAAACAGATTTGAGAATCGGTGTTAATGATTGTCGCGATTATACCAATGGTAATAATGTTGTTTAAACAATCTCATGTTACATACGTCCTATGTAAAGAATTATGTAGCATCCATAAGTGTTTGTATATTATCACTAATCACTATATCAATTAAATGATAAATTTTTAAATTATGAATATCTATGATCATGATTCAATCTGAATTGAATTTTTGGTCAATTTATAGGTTTGGTCAGACAACTCATTGGTGAAGAAGATGTGCTGAAACGTTTGAGAAACCAGGGTAGCTAG

Protein sequence

>MS.gene38100.t1

MESLVSKTRMVRGREEVYVAAMPLRASKGPPQLLMSAAYSLNIWDLQHFMVIIKPSSPSKVLVFDFQPKDPEDIYAALAVLSGRAVPGAVLVRKLKRLPRSKCWLVGYAEEDAVEMASEFNKKWETDLRIGVNDCRDYTNGLVRQLIGEEDVLKRLRNQGS