AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene43587


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 15 sgRNAs with CRISPR-Local

Find 20 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACATTTCTATTCCATTCT+TGG 0.177100 2.2:+66012162 MS.gene43587:CDS
CATACAGAGGGCCAAGTTGC+AGG 0.331211 2.2:+66012244 MS.gene43587:CDS
ACTACTCCATTGTTGATGAT+TGG 0.342996 2.2:+66012212 MS.gene43587:CDS
ACACAAGGACATACTCGAAT+GGG 0.501906 2.2:+66012581 MS.gene43587:CDS
ACTCCATTGTTGATGATTGG+AGG 0.510049 2.2:+66012215 MS.gene43587:CDS
TATGTGAATACCCTGCAACT+TGG 0.536092 2.2:-66012255 None:intergenic
TTGTTGATGATTGGAGGCAG+TGG 0.574543 2.2:+66012221 MS.gene43587:CDS
CATTCTTGGATACAAAAGCA+TGG 0.590619 2.2:+66012176 MS.gene43587:CDS
ATACAGAGGGCCAAGTTGCA+GGG 0.592654 2.2:+66012245 MS.gene43587:CDS
TACACAAGGACATACTCGAA+TGG 0.594245 2.2:+66012580 MS.gene43587:CDS
TGGAGGCAGTGGCATACAGA+GGG 0.634579 2.2:+66012232 MS.gene43587:CDS
CTGCCTCCAATCATCAACAA+TGG 0.635469 2.2:-66012218 None:intergenic
GATGACATTTGCAACTGTGA+AGG 0.656552 2.2:+66012603 MS.gene43587:CDS
TTGGAGGCAGTGGCATACAG+AGG 0.686415 2.2:+66012231 MS.gene43587:CDS
TGTGCAAGTATAGATACACA+AGG 0.695426 2.2:+66012566 MS.gene43587:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATCTTATAAATAGTTGAA+GGG - chr2.2:66012418-66012437 None:intergenic 15.0%
!! TATATCTTATAAATAGTTGA+AGG - chr2.2:66012419-66012438 None:intergenic 15.0%
!!! TTCTTTTTTCTTTTCTGAAA+TGG + chr2.2:66012335-66012354 MS.gene43587:intron 20.0%
! TCAACAAACGTTAATGTATT+TGG + chr2.2:66012453-66012472 MS.gene43587:intron 25.0%
TGACATTTCTATTCCATTCT+TGG + chr2.2:66012162-66012181 MS.gene43587:CDS 30.0%
CATTCTTGGATACAAAAGCA+TGG + chr2.2:66012176-66012195 MS.gene43587:CDS 35.0%
TGTGCAAGTATAGATACACA+AGG + chr2.2:66012566-66012585 MS.gene43587:intron 35.0%
! CATGCTTTTGTATCCAAGAA+TGG - chr2.2:66012178-66012197 None:intergenic 35.0%
!! ACTACTCCATTGTTGATGAT+TGG + chr2.2:66012212-66012231 MS.gene43587:CDS 35.0%
ACACAAGGACATACTCGAAT+GGG + chr2.2:66012581-66012600 MS.gene43587:CDS 40.0%
TACACAAGGACATACTCGAA+TGG + chr2.2:66012580-66012599 MS.gene43587:CDS 40.0%
TATGTGAATACCCTGCAACT+TGG - chr2.2:66012258-66012277 None:intergenic 40.0%
TGACATTTGCAACTGTGAAG+TGG + chr2.2:66012605-66012624 MS.gene43587:CDS 40.0%
!! ACTCCATTGTTGATGATTGG+AGG + chr2.2:66012215-66012234 MS.gene43587:CDS 40.0%
CTGCCTCCAATCATCAACAA+TGG - chr2.2:66012221-66012240 None:intergenic 45.0%
!! TTGTTGATGATTGGAGGCAG+TGG + chr2.2:66012221-66012240 MS.gene43587:CDS 45.0%
ATACAGAGGGCCAAGTTGCA+GGG + chr2.2:66012245-66012264 MS.gene43587:CDS 50.0%
CATACAGAGGGCCAAGTTGC+AGG + chr2.2:66012244-66012263 MS.gene43587:CDS 55.0%
TGGAGGCAGTGGCATACAGA+GGG + chr2.2:66012232-66012251 MS.gene43587:CDS 55.0%
TTGGAGGCAGTGGCATACAG+AGG + chr2.2:66012231-66012250 MS.gene43587:CDS 55.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 66012153 66012624 66012153 ID=MS.gene43587
chr2.2 mRNA 66012153 66012624 66012153 ID=MS.gene43587.t1;Parent=MS.gene43587
chr2.2 exon 66012153 66012266 66012153 ID=MS.gene43587.t1.exon1;Parent=MS.gene43587.t1
chr2.2 CDS 66012153 66012266 66012153 ID=cds.MS.gene43587.t1;Parent=MS.gene43587.t1
chr2.2 exon 66012579 66012624 66012579 ID=MS.gene43587.t1.exon2;Parent=MS.gene43587.t1
chr2.2 CDS 66012579 66012624 66012579 ID=cds.MS.gene43587.t1;Parent=MS.gene43587.t1
Gene Sequence

>MS.gene43587

TGGCGATCATGACATTTCTATTCCATTCTTGGATACAAAAGCATGGATAAAATCTTTAAACTACTCCATTGTTGATGATTGGAGGCAGTGGCATACAGAGGGCCAAGTTGCAGGGTATTCACATAAATTTTACTAGAATTATTCTATTTTTTCTTTAAGAAAGTGTTGCTAACATTTTTTTTTTCTTTTTTCTTTTCTGAAATGGTCTTTCTTAAAGTAAGAGTTTACAGTTTAATTCAAAAAGTGATCAATAATTTTTACTCCCTTCAACTATTTATAAGATATATTATATATATTTAGTCAACAAACGTTAATGTATTTGGTTCAAAATTTGAATCAAATATATCAAATTTTGTTGATTCAAATGTCATCTATCTAAAAAAAACAAATTAATTTAATATTTATTTTATTTTTGTGCAAGTATAGATACACAAGGACATACTCGAATGGGATGACATTTGCAACTGTGAAG

Protein sequence

>MS.gene43587.t1

WRSUHFYSILGYKSMDKIFKLLHCUULEAVAYRGPSCRIHKDILEWDDICNCE