Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49772.t1 | XP_013446943.1 | 99.7 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.00E+00 | 1146.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49772.t1 | Q941L3 | 70.9 | 578 | 164 | 2 | 1 | 577 | 1 | 575 | 3.4e-231 | 802.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49772.t1 | A0A072U553 | 99.7 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.0e+00 | 1146.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene49772 | 0.809318 | 2.01E-50 | -1.69E-46 |
| MS.gene049208 | MS.gene49772 | 0.822157 | 2.75E-53 | -1.69E-46 |
| MS.gene049368 | MS.gene49772 | 0.812713 | 3.69E-51 | -1.69E-46 |
| MS.gene049822 | MS.gene49772 | 0.821588 | 3.73E-53 | -1.69E-46 |
| MS.gene049861 | MS.gene49772 | 0.822163 | 2.74E-53 | -1.69E-46 |
| MS.gene04991 | MS.gene49772 | -0.810381 | 1.19E-50 | -1.69E-46 |
| MS.gene050114 | MS.gene49772 | 0.857816 | 1.26E-62 | -1.69E-46 |
| MS.gene050317 | MS.gene49772 | 0.840559 | 8.08E-58 | -1.69E-46 |
| MS.gene050439 | MS.gene49772 | 0.809555 | 1.79E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene49772 | 0.817802 | 2.73E-52 | -1.69E-46 |
| MS.gene050838 | MS.gene49772 | 0.800112 | 1.68E-48 | -1.69E-46 |
| MS.gene050941 | MS.gene49772 | 0.808443 | 3.09E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene49772 | 0.810695 | 1.01E-50 | -1.69E-46 |
| MS.gene051184 | MS.gene49772 | 0.836496 | 9.04E-57 | -1.69E-46 |
| MS.gene051655 | MS.gene49772 | 0.825504 | 4.52E-54 | -1.69E-46 |
| MS.gene051676 | MS.gene49772 | 0.829286 | 5.60E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene49772 | 0.843564 | 1.30E-58 | -1.69E-46 |
| MS.gene052052 | MS.gene49772 | 0.827193 | 1.79E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene49772 | 0.806395 | 8.40E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene49772 | 0.860225 | 2.40E-63 | -1.69E-46 |
| MS.gene052609 | MS.gene49772 | 0.812662 | 3.79E-51 | -1.69E-46 |
| MS.gene052932 | MS.gene49772 | 0.823591 | 1.28E-53 | -1.69E-46 |
| MS.gene053058 | MS.gene49772 | 0.802939 | 4.42E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene49772 | 0.80652 | 7.90E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene49772 | 0.837074 | 6.44E-57 | -1.69E-46 |
| MS.gene053879 | MS.gene49772 | 0.813205 | 2.88E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene49772 | 0.832919 | 7.18E-56 | -1.69E-46 |
| MS.gene05469 | MS.gene49772 | 0.816265 | 6.05E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene49772 | 0.812093 | 5.04E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene49772 | 0.813739 | 2.20E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene49772 | 0.828087 | 1.09E-54 | -1.69E-46 |
| MS.gene055313 | MS.gene49772 | 0.832647 | 8.39E-56 | -1.69E-46 |
| MS.gene055314 | MS.gene49772 | 0.822357 | 2.47E-53 | -1.69E-46 |
| MS.gene055603 | MS.gene49772 | 0.854997 | 8.48E-62 | -1.69E-46 |
| MS.gene055864 | MS.gene49772 | 0.833116 | 6.42E-56 | -1.69E-46 |
| MS.gene056045 | MS.gene49772 | 0.806654 | 7.40E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene49772 | 0.819502 | 1.12E-52 | -1.69E-46 |
| MS.gene056403 | MS.gene49772 | 0.825837 | 3.77E-54 | -1.69E-46 |
| MS.gene056605 | MS.gene49772 | 0.815833 | 7.55E-52 | -1.69E-46 |
| MS.gene056638 | MS.gene49772 | 0.820697 | 5.98E-53 | -1.69E-46 |
| MS.gene056787 | MS.gene49772 | 0.842747 | 2.14E-58 | -1.69E-46 |
| MS.gene056849 | MS.gene49772 | 0.80092 | 1.15E-48 | -1.69E-46 |
| MS.gene056986 | MS.gene49772 | 0.849771 | 2.61E-60 | -1.69E-46 |
| MS.gene057360 | MS.gene49772 | 0.821773 | 3.38E-53 | -1.69E-46 |
| MS.gene057999 | MS.gene49772 | 0.828908 | 6.92E-55 | -1.69E-46 |
| MS.gene058278 | MS.gene49772 | 0.815769 | 7.80E-52 | -1.69E-46 |
| MS.gene058429 | MS.gene49772 | 0.808639 | 2.81E-50 | -1.69E-46 |
| MS.gene058639 | MS.gene49772 | 0.808444 | 3.09E-50 | -1.69E-46 |
| MS.gene058758 | MS.gene49772 | 0.832966 | 6.99E-56 | -1.69E-46 |
| MS.gene059144 | MS.gene49772 | 0.838822 | 2.29E-57 | -1.69E-46 |
| MS.gene059360 | MS.gene49772 | 0.81253 | 4.05E-51 | -1.69E-46 |
| MS.gene059623 | MS.gene49772 | 0.802346 | 5.86E-49 | -1.69E-46 |
| MS.gene059719 | MS.gene49772 | 0.822673 | 2.09E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene49772 | 0.822921 | 1.83E-53 | -1.69E-46 |
| MS.gene06011 | MS.gene49772 | 0.833655 | 4.71E-56 | -1.69E-46 |
| MS.gene060191 | MS.gene49772 | 0.80505 | 1.61E-49 | -1.69E-46 |
| MS.gene060763 | MS.gene49772 | 0.80404 | 2.61E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene49772 | 0.802499 | 5.45E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene49772 | 0.824249 | 8.94E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene49772 | 0.801909 | 7.21E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene49772 | 0.821015 | 5.05E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene49772 | MS.gene78319 | PPI |
| MS.gene072428 | MS.gene49772 | PPI |
| MS.gene29288 | MS.gene49772 | PPI |
| MS.gene066539 | MS.gene49772 | PPI |
| MS.gene53726 | MS.gene49772 | PPI |
| MS.gene80471 | MS.gene49772 | PPI |
| MS.gene43347 | MS.gene49772 | PPI |
| MS.gene49772 | MS.gene41325 | PPI |
| MS.gene98925 | MS.gene49772 | PPI |
| MS.gene49772 | MS.gene70112 | PPI |
| MS.gene037909 | MS.gene49772 | PPI |
| MS.gene072429 | MS.gene49772 | PPI |
| MS.gene78320 | MS.gene49772 | PPI |
| MS.gene49772 | MS.gene066540 | PPI |
| MS.gene000424 | MS.gene49772 | PPI |
| MS.gene030511 | MS.gene49772 | PPI |
| MS.gene42007 | MS.gene49772 | PPI |
| MS.gene70112 | MS.gene49772 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49772.t1 | MTR_8g096190 | 99.655 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.0 | 1187 |
| MS.gene49772.t1 | MTR_8g096190 | 99.636 | 549 | 2 | 0 | 1 | 549 | 1 | 549 | 0.0 | 1127 |
| MS.gene49772.t1 | MTR_8g096190 | 98.846 | 520 | 6 | 0 | 1 | 520 | 1 | 520 | 0.0 | 1063 |
| MS.gene49772.t1 | MTR_1g048160 | 60.727 | 578 | 217 | 5 | 1 | 577 | 1 | 569 | 0.0 | 721 |
| MS.gene49772.t1 | MTR_7g114130 | 60.381 | 578 | 223 | 4 | 1 | 577 | 1 | 573 | 0.0 | 717 |
| MS.gene49772.t1 | MTR_7g114100 | 54.503 | 433 | 151 | 9 | 150 | 577 | 13 | 404 | 1.29e-147 | 432 |
| MS.gene49772.t1 | MTR_7g033100 | 32.667 | 300 | 154 | 12 | 295 | 568 | 59 | 336 | 1.24e-24 | 107 |
| MS.gene49772.t1 | MTR_3g075530 | 33.219 | 292 | 148 | 11 | 306 | 570 | 55 | 326 | 1.53e-24 | 107 |
| MS.gene49772.t1 | MTR_7g033100 | 32.441 | 299 | 156 | 11 | 295 | 568 | 59 | 336 | 2.32e-24 | 105 |
| MS.gene49772.t1 | MTR_6g021730 | 32.867 | 286 | 147 | 10 | 308 | 568 | 91 | 356 | 4.47e-24 | 106 |
| MS.gene49772.t1 | MTR_8g447220 | 30.961 | 281 | 148 | 10 | 316 | 571 | 53 | 312 | 2.82e-23 | 101 |
| MS.gene49772.t1 | MTR_8g447220 | 31.655 | 278 | 148 | 10 | 316 | 570 | 53 | 311 | 6.08e-23 | 101 |
| MS.gene49772.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 119 | 354 | 1.22e-22 | 101 |
| MS.gene49772.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 119 | 354 | 1.36e-22 | 101 |
| MS.gene49772.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 116 | 351 | 1.62e-22 | 101 |
| MS.gene49772.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 39 | 342 | 3.39e-22 | 100 |
| MS.gene49772.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 39 | 342 | 3.55e-22 | 99.8 |
| MS.gene49772.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 56 | 359 | 4.05e-22 | 99.8 |
| MS.gene49772.t1 | MTR_8g089670 | 34.375 | 256 | 124 | 10 | 337 | 568 | 126 | 361 | 9.26e-22 | 99.0 |
| MS.gene49772.t1 | MTR_6g092020 | 31.525 | 295 | 153 | 15 | 307 | 575 | 36 | 307 | 1.76e-21 | 97.4 |
| MS.gene49772.t1 | MTR_2g063857 | 30.872 | 298 | 154 | 12 | 307 | 575 | 57 | 331 | 1.84e-21 | 97.4 |
| MS.gene49772.t1 | MTR_2g063843 | 31.597 | 288 | 148 | 14 | 307 | 568 | 49 | 313 | 2.13e-21 | 97.1 |
| MS.gene49772.t1 | MTR_2g063857 | 31.058 | 293 | 146 | 12 | 307 | 568 | 57 | 324 | 4.03e-21 | 95.9 |
| MS.gene49772.t1 | MTR_6g092100 | 30.526 | 285 | 152 | 10 | 315 | 575 | 1 | 263 | 1.23e-20 | 93.2 |
| MS.gene49772.t1 | MTR_2g063907 | 30.502 | 259 | 132 | 10 | 343 | 575 | 81 | 317 | 1.38e-18 | 88.6 |
| MS.gene49772.t1 | MTR_7g081160 | 34.973 | 183 | 91 | 8 | 343 | 508 | 14 | 185 | 1.93e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene49772.t1 | AT5G61910 | 70.761 | 578 | 165 | 2 | 1 | 577 | 769 | 1343 | 0.0 | 829 |
| MS.gene49772.t1 | AT5G61900 | 70.761 | 578 | 165 | 2 | 1 | 577 | 1 | 575 | 0.0 | 824 |
| MS.gene49772.t1 | AT5G61900 | 70.761 | 578 | 165 | 2 | 1 | 577 | 1 | 575 | 0.0 | 824 |
| MS.gene49772.t1 | AT5G07300 | 68.056 | 576 | 183 | 1 | 1 | 575 | 1 | 576 | 0.0 | 813 |
| MS.gene49772.t1 | AT1G08860 | 60.309 | 582 | 222 | 5 | 1 | 577 | 1 | 578 | 0.0 | 714 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 107 | 344 | 2.90e-28 | 118 |
| MS.gene49772.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 107 | 344 | 3.94e-28 | 117 |
| MS.gene49772.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene49772.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene49772.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene49772.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene49772.t1 | AT3G01650 | 32.414 | 290 | 145 | 10 | 310 | 568 | 116 | 385 | 4.62e-24 | 106 |
| MS.gene49772.t1 | AT3G01650 | 32.414 | 290 | 145 | 10 | 310 | 568 | 116 | 385 | 4.62e-24 | 106 |
| MS.gene49772.t1 | AT1G79380 | 30.605 | 281 | 153 | 12 | 316 | 573 | 52 | 313 | 3.97e-21 | 96.3 |
| MS.gene49772.t1 | AT5G63970 | 31.061 | 264 | 139 | 9 | 337 | 577 | 31 | 274 | 2.60e-19 | 90.5 |
| MS.gene49772.t1 | AT5G63970 | 31.061 | 264 | 139 | 9 | 337 | 577 | 31 | 274 | 2.60e-19 | 90.5 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCATAAAGAACCATTAT+TGG | 0.170610 | 4.4:-7224374 | None:intergenic |
| AGTACAGTTTGTTCCATTTC+GGG | 0.198239 | 4.4:+7233610 | MS.gene49772:CDS |
| CTTACTCTTTGGTATGAATT+TGG | 0.198746 | 4.4:-7230161 | None:intergenic |
| CGATTTCCTACGTGGGGATT+TGG | 0.249110 | 4.4:+7230300 | MS.gene49772:CDS |
| TTAGAAGAACAACAATTTCT+TGG | 0.262270 | 4.4:+7225546 | MS.gene49772:CDS |
| TAGTACAGTTTGTTCCATTT+CGG | 0.270483 | 4.4:+7233609 | MS.gene49772:CDS |
| GGGATTTGAATTGAACTTCA+TGG | 0.330327 | 4.4:+7229028 | MS.gene49772:CDS |
| GTATCAAAAGTTGTGGAAAA+TGG | 0.333423 | 4.4:+7226189 | MS.gene49772:CDS |
| AAGTATTCAATATACTTTCC+TGG | 0.337996 | 4.4:+7228992 | MS.gene49772:CDS |
| TCTTTACATGACTAGAGATT+TGG | 0.338099 | 4.4:-7233806 | None:intergenic |
| ACTTTCCTGGATTACTTGGC+TGG | 0.341165 | 4.4:+7229005 | MS.gene49772:CDS |
| GAGCTGGAGAAGCTCTATTC+TGG | 0.347750 | 4.4:+7228791 | MS.gene49772:CDS |
| TTGCATTACATTGATCCTTC+AGG | 0.350303 | 4.4:+7230134 | MS.gene49772:CDS |
| AATGGAAAACATGATTTGTT+GGG | 0.354279 | 4.4:+7228660 | MS.gene49772:CDS |
| TGCCGGAGGTCCACTGAATC+CGG | 0.359355 | 4.4:+7222582 | MS.gene49772:CDS |
| GCGTCGTTGGAAGTGTTTCC+CGG | 0.363595 | 4.4:-7222601 | None:intergenic |
| GATACTCAGGTGTACAGATT+TGG | 0.363618 | 4.4:+7226037 | MS.gene49772:CDS |
| ATATACTTTCCTGGATTACT+TGG | 0.364628 | 4.4:+7229001 | MS.gene49772:CDS |
| TCCCATTGTTTCAACTTGAA+TGG | 0.386734 | 4.4:+7230348 | MS.gene49772:CDS |
| GTGTACAGATTTGGAATATA+GGG | 0.392511 | 4.4:+7226046 | MS.gene49772:CDS |
| TTCTTACTGTGATTATCAAC+AGG | 0.392780 | 4.4:-7230617 | None:intergenic |
| TCAATGTAATGCAAAGAATC+TGG | 0.395751 | 4.4:-7230125 | None:intergenic |
| GCCGGTGGCCGTACGGCAAT+CGG | 0.399080 | 4.4:+7222553 | MS.gene49772:CDS |
| CAATGGAAAACATGATTTGT+TGG | 0.408884 | 4.4:+7228659 | MS.gene49772:CDS |
| GGTGTACAGATTTGGAATAT+AGG | 0.414115 | 4.4:+7226045 | MS.gene49772:CDS |
| CTACTTTGAACATCCCGAAA+TGG | 0.420882 | 4.4:-7233623 | None:intergenic |
| TTCCACCATTTGCTACAGAT+TGG | 0.430209 | 4.4:-7230581 | None:intergenic |
| ATAATGGTTCTTTATGCAAA+AGG | 0.434192 | 4.4:+7224377 | MS.gene49772:CDS |
| ACCATTTGCTACAGATTGGC+TGG | 0.436179 | 4.4:-7230577 | None:intergenic |
| GCAGACATCTCTGGTGGAGC+TGG | 0.441893 | 4.4:+7228775 | MS.gene49772:CDS |
| TGATCGAGATGTGCTCTCTA+AGG | 0.450912 | 4.4:+7222793 | MS.gene49772:CDS |
| CAGAAAACTGCAGACATCTC+TGG | 0.451157 | 4.4:+7228766 | MS.gene49772:intron |
| TCCATTCAAGTTGAAACAAT+GGG | 0.454390 | 4.4:-7230349 | None:intergenic |
| TCCCGGATTCAGTGGACCTC+CGG | 0.455624 | 4.4:-7222584 | None:intergenic |
| GATAGGCTTGAAAGTTCATC+TGG | 0.456547 | 4.4:+7233566 | MS.gene49772:CDS |
| TTTGGAGCACGACCGATTGA+TGG | 0.458957 | 4.4:+7230318 | MS.gene49772:CDS |
| GAAGTTATCGACGGCGTCGT+TGG | 0.460192 | 4.4:-7222614 | None:intergenic |
| CGATGTCGCCGGTGGCCGTA+CGG | 0.467233 | 4.4:+7222546 | MS.gene49772:CDS |
| CTGCTTAACGTTTCTCTCGC+AGG | 0.467436 | 4.4:+7230510 | MS.gene49772:CDS |
| CTTTCCTGGATTACTTGGCT+GGG | 0.475384 | 4.4:+7229006 | MS.gene49772:CDS |
| AGACAAGATTCTACCAGATC+TGG | 0.479706 | 4.4:+7225942 | MS.gene49772:CDS |
| CTCCTCCTACGCCAACGATA+AGG | 0.479761 | 4.4:-7233369 | None:intergenic |
| GCCGATTGCCGTACGGCCAC+CGG | 0.479897 | 4.4:-7222554 | None:intergenic |
| TTGCAGGTAGAAGGCATTCA+AGG | 0.481279 | 4.4:+7230462 | MS.gene49772:intron |
| TTTCCTGGATTACTTGGCTG+GGG | 0.488293 | 4.4:+7229007 | MS.gene49772:CDS |
| ATTGCAGCATTATTTATGAC+AGG | 0.495261 | 4.4:-7230549 | None:intergenic |
| ACGGCAATCGGCGGAACTGC+CGG | 0.496253 | 4.4:+7222565 | MS.gene49772:CDS |
| ATCCTTATCGTTGGCGTAGG+AGG | 0.496936 | 4.4:+7233367 | MS.gene49772:CDS |
| TGTTGTTTACCAGCTTCAAA+TGG | 0.499401 | 4.4:+7230089 | MS.gene49772:intron |
| AATGGAGCACTTGAGGAACT+TGG | 0.502480 | 4.4:+7224404 | MS.gene49772:CDS |
| AGGAGCTGATTTCAAAGAGA+TGG | 0.503459 | 4.4:+7233387 | MS.gene49772:CDS |
| CCAACGATAAGGATTGATAA+TGG | 0.504163 | 4.4:-7233358 | None:intergenic |
| GCTGTAGAGTGATCCAATAA+TGG | 0.504399 | 4.4:+7224361 | MS.gene49772:intron |
| TGGATGCAGACAAAGGAGAT+AGG | 0.507223 | 4.4:+7233549 | MS.gene49772:CDS |
| TGGAGTACCTGTAAAATCAA+TGG | 0.509634 | 4.4:-7229054 | None:intergenic |
| GCCAGCCAATCTGTAGCAAA+TGG | 0.513378 | 4.4:+7230576 | MS.gene49772:CDS |
| AAGCTCTATTCTGGTGGCCA+AGG | 0.515420 | 4.4:+7228800 | MS.gene49772:CDS |
| TTTATTAGTATCAAAAGTTG+TGG | 0.517406 | 4.4:+7226182 | MS.gene49772:CDS |
| TGCTACAACTTCAATAGCAA+TGG | 0.517499 | 4.4:+7228642 | MS.gene49772:CDS |
| AAGTTGAAACAATGGGAGAC+AGG | 0.519091 | 4.4:-7230342 | None:intergenic |
| CCATTATCAATCCTTATCGT+TGG | 0.521967 | 4.4:+7233358 | MS.gene49772:CDS |
| GGCAATTATTGAGGTTGGAG+AGG | 0.522341 | 4.4:+7230251 | MS.gene49772:intron |
| CGTACGGCCACCGGCGACAT+CGG | 0.526840 | 4.4:-7222545 | None:intergenic |
| GCCGGAGGTCCACTGAATCC+GGG | 0.531146 | 4.4:+7222583 | MS.gene49772:CDS |
| TTCCTGGATTACTTGGCTGG+GGG | 0.531776 | 4.4:+7229008 | MS.gene49772:CDS |
| TTCCATTCAAGTTGAAACAA+TGG | 0.534177 | 4.4:-7230350 | None:intergenic |
| AATTGCTGCTCCGATGTCGC+CGG | 0.549086 | 4.4:+7222535 | MS.gene49772:CDS |
| TATGAATTTGGCCGTCCTGA+AGG | 0.556160 | 4.4:-7230149 | None:intergenic |
| AAAATTTGTCCACAAATAGT+CGG | 0.556612 | 4.4:-7228965 | None:intergenic |
| TGAGAATCATGACCCACGGC+AGG | 0.557508 | 4.4:-7228842 | None:intergenic |
| GGGTCATGATTCTCAGACCA+AGG | 0.557519 | 4.4:+7228850 | MS.gene49772:CDS |
| TGGGAGACAGGACCATCAAT+CGG | 0.559133 | 4.4:-7230330 | None:intergenic |
| GAAGTGTTTCCCGGATTCAG+TGG | 0.560239 | 4.4:-7222592 | None:intergenic |
| TCAATCCTTATCGTTGGCGT+AGG | 0.573889 | 4.4:+7233364 | MS.gene49772:CDS |
| CGATCTGAGAGAAGAGACCT+TGG | 0.574746 | 4.4:-7222654 | None:intergenic |
| TGCTGCTCCGATGTCGCCGG+TGG | 0.582433 | 4.4:+7222538 | MS.gene49772:CDS |
| ATGGAAAACATGATTTGTTG+GGG | 0.584941 | 4.4:+7228661 | MS.gene49772:CDS |
| AGAACAACAATTTCTTGGTG+AGG | 0.589436 | 4.4:+7225551 | MS.gene49772:CDS |
| GACAAGCGATTTCCTACGTG+GGG | 0.590517 | 4.4:+7230294 | MS.gene49772:CDS |
| AGATGCACTTGTTAAAGCAT+CGG | 0.592040 | 4.4:+7233330 | MS.gene49772:CDS |
| CAGTTCCGCCGATTGCCGTA+CGG | 0.592626 | 4.4:-7222561 | None:intergenic |
| TACATGACTAGAGATTTGGT+TGG | 0.595044 | 4.4:-7233802 | None:intergenic |
| AATTCAAAACTACTTCCGTC+CGG | 0.595748 | 4.4:-7224427 | None:intergenic |
| CTACTAGGATGGAGTTGTGA+CGG | 0.599974 | 4.4:+7233291 | MS.gene49772:intron |
| TGTTCAACAAGTAGGAAGCA+AGG | 0.601240 | 4.4:+7226287 | MS.gene49772:CDS |
| TTCAAGTCTCGTGGCTACCA+AGG | 0.609125 | 4.4:+7222637 | MS.gene49772:CDS |
| GAGCACTTGAGGAACTTGGC+CGG | 0.609390 | 4.4:+7224408 | MS.gene49772:CDS |
| ACTTGAGGAACTTGGCCGGA+CGG | 0.609426 | 4.4:+7224412 | MS.gene49772:CDS |
| AGCTGATTTCAAAGAGATGG+AGG | 0.615204 | 4.4:+7233390 | MS.gene49772:CDS |
| TGAATTCGTTGAATCCGACA+TGG | 0.617366 | 4.4:+7224444 | MS.gene49772:CDS |
| CAGACAAGCGATTTCCTACG+TGG | 0.619290 | 4.4:+7230292 | MS.gene49772:CDS |
| CAGATCTTGGATGCAGACAA+AGG | 0.620101 | 4.4:+7233542 | MS.gene49772:intron |
| GTTTCACAACGCAGACGTGA+AGG | 0.620590 | 4.4:+7224635 | MS.gene49772:CDS |
| AAAACTGCAGACATCTCTGG+TGG | 0.621038 | 4.4:+7228769 | MS.gene49772:CDS |
| TTGTAGCACTCTATTAACAA+AGG | 0.621510 | 4.4:-7228627 | None:intergenic |
| GTTTGTTTAGTAATCCATGT+CGG | 0.622631 | 4.4:-7224458 | None:intergenic |
| CTACAGTAGAGATGATACTC+AGG | 0.624513 | 4.4:+7226024 | MS.gene49772:CDS |
| CGATAAGGATTGATAATGGT+AGG | 0.628743 | 4.4:-7233354 | None:intergenic |
| AGAATGATCACAATCCAACA+TGG | 0.632845 | 4.4:+7226247 | MS.gene49772:CDS |
| GGTGGCCGTACGGCAATCGG+CGG | 0.635146 | 4.4:+7222556 | MS.gene49772:CDS |
| TGTAATGCAAAGAATCTGGA+AGG | 0.637807 | 4.4:-7230121 | None:intergenic |
| CTGGAGAAGCTCTATTCTGG+TGG | 0.643477 | 4.4:+7228794 | MS.gene49772:CDS |
| AGGCATTCAAGGAATCATGA+TGG | 0.645806 | 4.4:+7230473 | MS.gene49772:CDS |
| GATAACTTCTTCAAGTCTCG+TGG | 0.649983 | 4.4:+7222628 | MS.gene49772:CDS |
| AGCCAATCTGTAGCAAATGG+TGG | 0.650165 | 4.4:+7230579 | MS.gene49772:CDS |
| GGTCTGAGAATCATGACCCA+CGG | 0.650774 | 4.4:-7228846 | None:intergenic |
| AGGTCTCTTCTCTCAGATCG+AGG | 0.653280 | 4.4:+7222657 | MS.gene49772:CDS |
| GGCAACTTGTGCATTATCTG+AGG | 0.654797 | 4.4:+7225572 | MS.gene49772:CDS |
| AGACAAGCGATTTCCTACGT+GGG | 0.662700 | 4.4:+7230293 | MS.gene49772:CDS |
| GCAATCGGCGGAACTGCCGG+AGG | 0.665003 | 4.4:+7222568 | MS.gene49772:CDS |
| ATTACATTGATCCTTCAGGA+CGG | 0.667479 | 4.4:+7230138 | MS.gene49772:CDS |
| ATTTGAATTGAACTTCATGG+TGG | 0.674358 | 4.4:+7229031 | MS.gene49772:CDS |
| GTGTATGCTGAGGAATGTGT+TGG | 0.678518 | 4.4:+7225993 | MS.gene49772:CDS |
| AGACTTGAAGAAGTTATCGA+CGG | 0.701794 | 4.4:-7222623 | None:intergenic |
| GAAGCTCAAAGTGTATGCTG+AGG | 0.705846 | 4.4:+7225983 | MS.gene49772:CDS |
| CGTGCTCCAAATCCCCACGT+AGG | 0.721272 | 4.4:-7230306 | None:intergenic |
| TGGAAGCAATACTTACTGTG+AGG | 0.795076 | 4.4:+7230368 | MS.gene49772:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 7222529 | 7233817 | 7222529 | ID=MS.gene49772 |
| chr4.4 | mRNA | 7222529 | 7233817 | 7222529 | ID=MS.gene49772.t1;Parent=MS.gene49772 |
| chr4.4 | exon | 7222529 | 7222678 | 7222529 | ID=MS.gene49772.t1.exon1;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7222529 | 7222678 | 7222529 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7222767 | 7222814 | 7222767 | ID=MS.gene49772.t1.exon2;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7222767 | 7222814 | 7222767 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7224368 | 7224516 | 7224368 | ID=MS.gene49772.t1.exon3;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7224368 | 7224516 | 7224368 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7224608 | 7224656 | 7224608 | ID=MS.gene49772.t1.exon4;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7224608 | 7224656 | 7224608 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7225537 | 7225593 | 7225537 | ID=MS.gene49772.t1.exon5;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7225537 | 7225593 | 7225537 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7225900 | 7226085 | 7225900 | ID=MS.gene49772.t1.exon6;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7225900 | 7226085 | 7225900 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7226174 | 7226308 | 7226174 | ID=MS.gene49772.t1.exon7;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7226174 | 7226308 | 7226174 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7228621 | 7228682 | 7228621 | ID=MS.gene49772.t1.exon8;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7228621 | 7228682 | 7228621 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7228769 | 7228871 | 7228769 | ID=MS.gene49772.t1.exon9;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7228769 | 7228871 | 7228769 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7228954 | 7229068 | 7228954 | ID=MS.gene49772.t1.exon10;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7228954 | 7229068 | 7228954 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7230102 | 7230178 | 7230102 | ID=MS.gene49772.t1.exon11;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7230102 | 7230178 | 7230102 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7230252 | 7230389 | 7230252 | ID=MS.gene49772.t1.exon12;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7230252 | 7230389 | 7230252 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7230468 | 7230632 | 7230468 | ID=MS.gene49772.t1.exon13;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7230468 | 7230632 | 7230468 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7233298 | 7233411 | 7233298 | ID=MS.gene49772.t1.exon14;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7233298 | 7233411 | 7233298 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7233545 | 7233641 | 7233545 | ID=MS.gene49772.t1.exon15;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7233545 | 7233641 | 7233545 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| chr4.4 | exon | 7233723 | 7233817 | 7233723 | ID=MS.gene49772.t1.exon16;Parent=MS.gene49772.t1 |
| chr4.4 | CDS | 7233723 | 7233817 | 7233723 | ID=cds.MS.gene49772.t1;Parent=MS.gene49772.t1 |
| Gene Sequence |
| Protein sequence |
