Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70112.t1 | XP_013446943.1 | 99.7 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.00E+00 | 1146.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70112.t1 | Q941L3 | 70.9 | 578 | 164 | 2 | 1 | 577 | 1 | 575 | 3.4e-231 | 802.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70112.t1 | A0A072U553 | 99.7 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.0e+00 | 1146.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049166 | MS.gene70112 | 0.808511 | 2.99E-50 | -1.69E-46 |
| MS.gene049208 | MS.gene70112 | 0.823767 | 1.16E-53 | -1.69E-46 |
| MS.gene049283 | MS.gene70112 | 0.80207 | 6.68E-49 | -1.69E-46 |
| MS.gene049368 | MS.gene70112 | 0.816201 | 6.25E-52 | -1.69E-46 |
| MS.gene049822 | MS.gene70112 | 0.817811 | 2.72E-52 | -1.69E-46 |
| MS.gene049861 | MS.gene70112 | 0.819389 | 1.19E-52 | -1.69E-46 |
| MS.gene04991 | MS.gene70112 | -0.812823 | 3.49E-51 | -1.69E-46 |
| MS.gene050114 | MS.gene70112 | 0.858394 | 8.49E-63 | -1.69E-46 |
| MS.gene050317 | MS.gene70112 | 0.837069 | 6.46E-57 | -1.69E-46 |
| MS.gene050439 | MS.gene70112 | 0.808209 | 3.47E-50 | -1.69E-46 |
| MS.gene050654 | MS.gene70112 | 0.818417 | 1.98E-52 | -1.69E-46 |
| MS.gene050941 | MS.gene70112 | 0.809051 | 2.29E-50 | -1.69E-46 |
| MS.gene051003 | MS.gene70112 | 0.810847 | 9.40E-51 | -1.69E-46 |
| MS.gene051184 | MS.gene70112 | 0.836203 | 1.07E-56 | -1.69E-46 |
| MS.gene051655 | MS.gene70112 | 0.82505 | 5.79E-54 | -1.69E-46 |
| MS.gene051676 | MS.gene70112 | 0.828658 | 7.95E-55 | -1.69E-46 |
| MS.gene051975 | MS.gene70112 | 0.845982 | 2.89E-59 | -1.69E-46 |
| MS.gene052052 | MS.gene70112 | 0.825174 | 5.41E-54 | -1.69E-46 |
| MS.gene052307 | MS.gene70112 | 0.807506 | 4.89E-50 | -1.69E-46 |
| MS.gene052434 | MS.gene70112 | 0.855232 | 7.24E-62 | -1.69E-46 |
| MS.gene052609 | MS.gene70112 | 0.813977 | 1.95E-51 | -1.69E-46 |
| MS.gene052932 | MS.gene70112 | 0.827611 | 1.42E-54 | -1.69E-46 |
| MS.gene053058 | MS.gene70112 | 0.803737 | 3.02E-49 | -1.69E-46 |
| MS.gene053298 | MS.gene70112 | 0.808572 | 2.90E-50 | -1.69E-46 |
| MS.gene053381 | MS.gene70112 | 0.831347 | 1.76E-55 | -1.69E-46 |
| MS.gene053879 | MS.gene70112 | 0.81344 | 2.56E-51 | -1.69E-46 |
| MS.gene054479 | MS.gene70112 | 0.836793 | 7.60E-57 | -1.69E-46 |
| MS.gene05469 | MS.gene70112 | 0.81803 | 2.43E-52 | -1.69E-46 |
| MS.gene054892 | MS.gene70112 | 0.813435 | 2.56E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene70112 | 0.815225 | 1.03E-51 | -1.69E-46 |
| MS.gene055311 | MS.gene70112 | 0.831237 | 1.87E-55 | -1.69E-46 |
| MS.gene055313 | MS.gene70112 | 0.836286 | 1.02E-56 | -1.69E-46 |
| MS.gene055314 | MS.gene70112 | 0.826944 | 2.05E-54 | -1.69E-46 |
| MS.gene055603 | MS.gene70112 | 0.852396 | 4.74E-61 | -1.69E-46 |
| MS.gene055864 | MS.gene70112 | 0.834128 | 3.58E-56 | -1.69E-46 |
| MS.gene056045 | MS.gene70112 | 0.808837 | 2.55E-50 | -1.69E-46 |
| MS.gene056181 | MS.gene70112 | 0.822218 | 2.66E-53 | -1.69E-46 |
| MS.gene056403 | MS.gene70112 | 0.826889 | 2.12E-54 | -1.69E-46 |
| MS.gene056605 | MS.gene70112 | 0.817711 | 2.86E-52 | -1.69E-46 |
| MS.gene056638 | MS.gene70112 | 0.818182 | 2.24E-52 | -1.69E-46 |
| MS.gene056787 | MS.gene70112 | 0.84437 | 7.88E-59 | -1.69E-46 |
| MS.gene056986 | MS.gene70112 | 0.850144 | 2.05E-60 | -1.69E-46 |
| MS.gene057360 | MS.gene70112 | 0.818728 | 1.68E-52 | -1.69E-46 |
| MS.gene057999 | MS.gene70112 | 0.828883 | 7.02E-55 | -1.69E-46 |
| MS.gene058278 | MS.gene70112 | 0.810901 | 9.15E-51 | -1.69E-46 |
| MS.gene058429 | MS.gene70112 | 0.808225 | 3.44E-50 | -1.69E-46 |
| MS.gene058638 | MS.gene70112 | 0.800433 | 1.44E-48 | -1.69E-46 |
| MS.gene058639 | MS.gene70112 | 0.808288 | 3.33E-50 | -1.69E-46 |
| MS.gene058758 | MS.gene70112 | 0.833894 | 4.10E-56 | -1.69E-46 |
| MS.gene059144 | MS.gene70112 | 0.836407 | 9.53E-57 | -1.69E-46 |
| MS.gene059360 | MS.gene70112 | 0.810614 | 1.06E-50 | -1.69E-46 |
| MS.gene059623 | MS.gene70112 | 0.802374 | 5.78E-49 | -1.69E-46 |
| MS.gene059719 | MS.gene70112 | 0.822102 | 2.84E-53 | -1.69E-46 |
| MS.gene06010 | MS.gene70112 | 0.826129 | 3.21E-54 | -1.69E-46 |
| MS.gene06011 | MS.gene70112 | 0.832278 | 1.04E-55 | -1.69E-46 |
| MS.gene060191 | MS.gene70112 | 0.812678 | 3.76E-51 | -1.69E-46 |
| MS.gene060763 | MS.gene70112 | 0.801871 | 7.34E-49 | -1.69E-46 |
| MS.gene060869 | MS.gene70112 | 0.804409 | 2.19E-49 | -1.69E-46 |
| MS.gene060933 | MS.gene70112 | 0.828049 | 1.12E-54 | -1.69E-46 |
| MS.gene060935 | MS.gene70112 | 0.805102 | 1.57E-49 | -1.69E-46 |
| MS.gene060937 | MS.gene70112 | 0.823512 | 1.33E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene066539 | MS.gene70112 | PPI |
| MS.gene80471 | MS.gene70112 | PPI |
| MS.gene43347 | MS.gene70112 | PPI |
| MS.gene29288 | MS.gene70112 | PPI |
| MS.gene70112 | MS.gene78319 | PPI |
| MS.gene98925 | MS.gene70112 | PPI |
| MS.gene037909 | MS.gene70112 | PPI |
| MS.gene072429 | MS.gene70112 | PPI |
| MS.gene49772 | MS.gene70112 | PPI |
| MS.gene78320 | MS.gene70112 | PPI |
| MS.gene000424 | MS.gene70112 | PPI |
| MS.gene70112 | MS.gene41325 | PPI |
| MS.gene030511 | MS.gene70112 | PPI |
| MS.gene42007 | MS.gene70112 | PPI |
| MS.gene70112 | MS.gene066540 | PPI |
| MS.gene53726 | MS.gene70112 | PPI |
| MS.gene70112 | MS.gene49772 | PPI |
| MS.gene072428 | MS.gene70112 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70112.t1 | MTR_8g096190 | 99.655 | 579 | 2 | 0 | 1 | 579 | 1 | 579 | 0.0 | 1187 |
| MS.gene70112.t1 | MTR_8g096190 | 99.636 | 549 | 2 | 0 | 1 | 549 | 1 | 549 | 0.0 | 1127 |
| MS.gene70112.t1 | MTR_8g096190 | 98.846 | 520 | 6 | 0 | 1 | 520 | 1 | 520 | 0.0 | 1063 |
| MS.gene70112.t1 | MTR_1g048160 | 60.727 | 578 | 217 | 5 | 1 | 577 | 1 | 569 | 0.0 | 721 |
| MS.gene70112.t1 | MTR_7g114130 | 60.381 | 578 | 223 | 4 | 1 | 577 | 1 | 573 | 0.0 | 717 |
| MS.gene70112.t1 | MTR_7g114100 | 54.503 | 433 | 151 | 9 | 150 | 577 | 13 | 404 | 1.29e-147 | 432 |
| MS.gene70112.t1 | MTR_7g033100 | 32.667 | 300 | 154 | 12 | 295 | 568 | 59 | 336 | 1.24e-24 | 107 |
| MS.gene70112.t1 | MTR_3g075530 | 33.219 | 292 | 148 | 11 | 306 | 570 | 55 | 326 | 1.53e-24 | 107 |
| MS.gene70112.t1 | MTR_7g033100 | 32.441 | 299 | 156 | 11 | 295 | 568 | 59 | 336 | 2.32e-24 | 105 |
| MS.gene70112.t1 | MTR_6g021730 | 32.867 | 286 | 147 | 10 | 308 | 568 | 91 | 356 | 4.47e-24 | 106 |
| MS.gene70112.t1 | MTR_8g447220 | 30.961 | 281 | 148 | 10 | 316 | 571 | 53 | 312 | 2.82e-23 | 101 |
| MS.gene70112.t1 | MTR_8g447220 | 31.655 | 278 | 148 | 10 | 316 | 570 | 53 | 311 | 6.08e-23 | 101 |
| MS.gene70112.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 119 | 354 | 1.22e-22 | 101 |
| MS.gene70112.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 119 | 354 | 1.36e-22 | 101 |
| MS.gene70112.t1 | MTR_3g086520 | 34.766 | 256 | 123 | 11 | 337 | 568 | 116 | 351 | 1.62e-22 | 101 |
| MS.gene70112.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 39 | 342 | 3.39e-22 | 100 |
| MS.gene70112.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 39 | 342 | 3.55e-22 | 99.8 |
| MS.gene70112.t1 | MTR_3g096520 | 30.887 | 327 | 174 | 13 | 279 | 576 | 56 | 359 | 4.05e-22 | 99.8 |
| MS.gene70112.t1 | MTR_8g089670 | 34.375 | 256 | 124 | 10 | 337 | 568 | 126 | 361 | 9.26e-22 | 99.0 |
| MS.gene70112.t1 | MTR_6g092020 | 31.525 | 295 | 153 | 15 | 307 | 575 | 36 | 307 | 1.76e-21 | 97.4 |
| MS.gene70112.t1 | MTR_2g063857 | 30.872 | 298 | 154 | 12 | 307 | 575 | 57 | 331 | 1.84e-21 | 97.4 |
| MS.gene70112.t1 | MTR_2g063843 | 31.597 | 288 | 148 | 14 | 307 | 568 | 49 | 313 | 2.13e-21 | 97.1 |
| MS.gene70112.t1 | MTR_2g063857 | 31.058 | 293 | 146 | 12 | 307 | 568 | 57 | 324 | 4.03e-21 | 95.9 |
| MS.gene70112.t1 | MTR_6g092100 | 30.526 | 285 | 152 | 10 | 315 | 575 | 1 | 263 | 1.23e-20 | 93.2 |
| MS.gene70112.t1 | MTR_2g063907 | 30.502 | 259 | 132 | 10 | 343 | 575 | 81 | 317 | 1.38e-18 | 88.6 |
| MS.gene70112.t1 | MTR_7g081160 | 34.973 | 183 | 91 | 8 | 343 | 508 | 14 | 185 | 1.93e-12 | 70.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70112.t1 | AT5G61910 | 70.761 | 578 | 165 | 2 | 1 | 577 | 769 | 1343 | 0.0 | 829 |
| MS.gene70112.t1 | AT5G61900 | 70.761 | 578 | 165 | 2 | 1 | 577 | 1 | 575 | 0.0 | 824 |
| MS.gene70112.t1 | AT5G61900 | 70.761 | 578 | 165 | 2 | 1 | 577 | 1 | 575 | 0.0 | 824 |
| MS.gene70112.t1 | AT5G07300 | 68.056 | 576 | 183 | 1 | 1 | 575 | 1 | 576 | 0.0 | 813 |
| MS.gene70112.t1 | AT1G08860 | 60.309 | 582 | 222 | 5 | 1 | 577 | 1 | 578 | 0.0 | 714 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 87 | 324 | 2.18e-28 | 118 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 107 | 344 | 2.90e-28 | 118 |
| MS.gene70112.t1 | AT1G67800 | 35.271 | 258 | 123 | 9 | 337 | 570 | 107 | 344 | 3.94e-28 | 117 |
| MS.gene70112.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene70112.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene70112.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene70112.t1 | AT5G14420 | 33.333 | 291 | 149 | 10 | 303 | 568 | 81 | 351 | 8.42e-26 | 111 |
| MS.gene70112.t1 | AT3G01650 | 32.414 | 290 | 145 | 10 | 310 | 568 | 116 | 385 | 4.62e-24 | 106 |
| MS.gene70112.t1 | AT3G01650 | 32.414 | 290 | 145 | 10 | 310 | 568 | 116 | 385 | 4.62e-24 | 106 |
| MS.gene70112.t1 | AT1G79380 | 30.605 | 281 | 153 | 12 | 316 | 573 | 52 | 313 | 3.97e-21 | 96.3 |
| MS.gene70112.t1 | AT5G63970 | 31.061 | 264 | 139 | 9 | 337 | 577 | 31 | 274 | 2.60e-19 | 90.5 |
| MS.gene70112.t1 | AT5G63970 | 31.061 | 264 | 139 | 9 | 337 | 577 | 31 | 274 | 2.60e-19 | 90.5 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGCATAAAGAACCATTAT+TGG | 0.170610 | 4.1:-7130380 | None:intergenic |
| AGTACAGTTTGTTCCATTTC+GGG | 0.198239 | 4.1:+7139109 | MS.gene70112:CDS |
| CTTACTCTTTGGTATGAATT+TGG | 0.198746 | 4.1:-7135656 | None:intergenic |
| CGATTTCCTACGTGGGGATT+TGG | 0.249110 | 4.1:+7135795 | MS.gene70112:CDS |
| TTAGAAGAACAACAATTTCT+TGG | 0.262270 | 4.1:+7131536 | MS.gene70112:CDS |
| TAGTACAGTTTGTTCCATTT+CGG | 0.270483 | 4.1:+7139108 | MS.gene70112:CDS |
| GGGATTTGAATTGAACTTCA+TGG | 0.330327 | 4.1:+7134521 | MS.gene70112:CDS |
| GTATCAAAAGTTGTGGAAAA+TGG | 0.333423 | 4.1:+7132179 | MS.gene70112:CDS |
| AAGTATTCAATATACTTTCC+TGG | 0.337996 | 4.1:+7134485 | MS.gene70112:CDS |
| TCTTTACATGACTAGAGATT+TGG | 0.338099 | 4.1:-7139305 | None:intergenic |
| ACTTTCCTGGATTACTTGGC+TGG | 0.341165 | 4.1:+7134498 | MS.gene70112:CDS |
| GAGCTGGAGAAGCTCTATTC+TGG | 0.347750 | 4.1:+7134284 | MS.gene70112:CDS |
| TTGCATTACATTGATCCTTC+AGG | 0.350303 | 4.1:+7135629 | MS.gene70112:CDS |
| AATGGAAAACATGATTTGTT+GGG | 0.354279 | 4.1:+7134153 | MS.gene70112:CDS |
| TGCCGGAGGTCCACTGAATC+CGG | 0.359355 | 4.1:+7128605 | MS.gene70112:CDS |
| GCGTCGTTGGAAGTGTTTCC+CGG | 0.363595 | 4.1:-7128624 | None:intergenic |
| GATACTCAGGTGTACAGATT+TGG | 0.363618 | 4.1:+7132027 | MS.gene70112:CDS |
| ATATACTTTCCTGGATTACT+TGG | 0.364628 | 4.1:+7134494 | MS.gene70112:CDS |
| TCCCATTGTTTCAACTTGAA+TGG | 0.386734 | 4.1:+7135843 | MS.gene70112:CDS |
| GTGTACAGATTTGGAATATA+GGG | 0.392511 | 4.1:+7132036 | MS.gene70112:CDS |
| TTCTTACTGTGATTATCAAC+AGG | 0.392780 | 4.1:-7136112 | None:intergenic |
| TCAATGTAATGCAAAGAATC+TGG | 0.395751 | 4.1:-7135620 | None:intergenic |
| GCCGGTGGCCGTACGGCAAT+CGG | 0.399080 | 4.1:+7128576 | MS.gene70112:CDS |
| CAATGGAAAACATGATTTGT+TGG | 0.408884 | 4.1:+7134152 | MS.gene70112:CDS |
| GGTGTACAGATTTGGAATAT+AGG | 0.414115 | 4.1:+7132035 | MS.gene70112:CDS |
| CTACTTTGAACATCCCGAAA+TGG | 0.420882 | 4.1:-7139122 | None:intergenic |
| TTCCACCATTTGCTACAGAT+TGG | 0.430209 | 4.1:-7136076 | None:intergenic |
| ATAATGGTTCTTTATGCAAA+AGG | 0.434192 | 4.1:+7130383 | MS.gene70112:CDS |
| ACCATTTGCTACAGATTGGC+TGG | 0.436179 | 4.1:-7136072 | None:intergenic |
| GCAGACATCTCTGGTGGAGC+TGG | 0.441893 | 4.1:+7134268 | MS.gene70112:CDS |
| TGATCGAGATGTGCTCTCTA+AGG | 0.450912 | 4.1:+7128816 | MS.gene70112:CDS |
| CAGAAAACTGCAGACATCTC+TGG | 0.451157 | 4.1:+7134259 | MS.gene70112:intron |
| TCCATTCAAGTTGAAACAAT+GGG | 0.454390 | 4.1:-7135844 | None:intergenic |
| TCCCGGATTCAGTGGACCTC+CGG | 0.455624 | 4.1:-7128607 | None:intergenic |
| GATAGGCTTGAAAGTTCATC+TGG | 0.456547 | 4.1:+7139065 | MS.gene70112:CDS |
| TTTGGAGCACGACCGATTGA+TGG | 0.458957 | 4.1:+7135813 | MS.gene70112:CDS |
| GAAGTTATCGACGGCGTCGT+TGG | 0.460192 | 4.1:-7128637 | None:intergenic |
| CGATGTCGCCGGTGGCCGTA+CGG | 0.467233 | 4.1:+7128569 | MS.gene70112:CDS |
| CTGCTTAACGTTTCTCTCGC+AGG | 0.467436 | 4.1:+7136005 | MS.gene70112:CDS |
| CTTTCCTGGATTACTTGGCT+GGG | 0.475384 | 4.1:+7134499 | MS.gene70112:CDS |
| AGACAAGATTCTACCAGATC+TGG | 0.479706 | 4.1:+7131932 | MS.gene70112:CDS |
| CTCCTCCTACGCCAACGATA+AGG | 0.479761 | 4.1:-7138868 | None:intergenic |
| GCCGATTGCCGTACGGCCAC+CGG | 0.479897 | 4.1:-7128577 | None:intergenic |
| TTGCAGGTAGAAGGCATTCA+AGG | 0.481279 | 4.1:+7135957 | MS.gene70112:intron |
| TTTCCTGGATTACTTGGCTG+GGG | 0.488293 | 4.1:+7134500 | MS.gene70112:CDS |
| ATTGCAGCATTATTTATGAC+AGG | 0.495261 | 4.1:-7136044 | None:intergenic |
| ACGGCAATCGGCGGAACTGC+CGG | 0.496253 | 4.1:+7128588 | MS.gene70112:CDS |
| ATCCTTATCGTTGGCGTAGG+AGG | 0.496936 | 4.1:+7138866 | MS.gene70112:CDS |
| TGTTGTTTACCAGCTTCAAA+TGG | 0.499401 | 4.1:+7135584 | MS.gene70112:intron |
| AATGGAGCACTTGAGGAACT+TGG | 0.502480 | 4.1:+7130410 | MS.gene70112:CDS |
| AGGAGCTGATTTCAAAGAGA+TGG | 0.503459 | 4.1:+7138886 | MS.gene70112:CDS |
| CCAACGATAAGGATTGATAA+TGG | 0.504163 | 4.1:-7138857 | None:intergenic |
| GCTGTAGAGTGATCCAATAA+TGG | 0.504399 | 4.1:+7130367 | MS.gene70112:intron |
| TGGATGCAGACAAAGGAGAT+AGG | 0.507223 | 4.1:+7139048 | MS.gene70112:CDS |
| TGGAGTACCTGTAAAATCAA+TGG | 0.509634 | 4.1:-7134547 | None:intergenic |
| GCCAGCCAATCTGTAGCAAA+TGG | 0.513378 | 4.1:+7136071 | MS.gene70112:CDS |
| AAGCTCTATTCTGGTGGCCA+AGG | 0.515420 | 4.1:+7134293 | MS.gene70112:CDS |
| TTTATTAGTATCAAAAGTTG+TGG | 0.517406 | 4.1:+7132172 | MS.gene70112:CDS |
| TGCTACAACTTCAATAGCAA+TGG | 0.517499 | 4.1:+7134135 | MS.gene70112:CDS |
| AAGTTGAAACAATGGGAGAC+AGG | 0.519091 | 4.1:-7135837 | None:intergenic |
| CCATTATCAATCCTTATCGT+TGG | 0.521967 | 4.1:+7138857 | MS.gene70112:CDS |
| GGCAATTATTGAGGTTGGAG+AGG | 0.522341 | 4.1:+7135746 | MS.gene70112:intron |
| CGTACGGCCACCGGCGACAT+CGG | 0.526840 | 4.1:-7128568 | None:intergenic |
| GCCGGAGGTCCACTGAATCC+GGG | 0.531146 | 4.1:+7128606 | MS.gene70112:CDS |
| TTCCTGGATTACTTGGCTGG+GGG | 0.531776 | 4.1:+7134501 | MS.gene70112:CDS |
| TTCCATTCAAGTTGAAACAA+TGG | 0.534177 | 4.1:-7135845 | None:intergenic |
| AATTGCTGCTCCGATGTCGC+CGG | 0.549086 | 4.1:+7128558 | MS.gene70112:CDS |
| TATGAATTTGGCCGTCCTGA+AGG | 0.556160 | 4.1:-7135644 | None:intergenic |
| AAAATTTGTCCACAAATAGT+CGG | 0.556612 | 4.1:-7134458 | None:intergenic |
| TGAGAATCATGACCCACGGC+AGG | 0.557508 | 4.1:-7134335 | None:intergenic |
| GGGTCATGATTCTCAGACCA+AGG | 0.557519 | 4.1:+7134343 | MS.gene70112:CDS |
| TGGGAGACAGGACCATCAAT+CGG | 0.559133 | 4.1:-7135825 | None:intergenic |
| GAAGTGTTTCCCGGATTCAG+TGG | 0.560239 | 4.1:-7128615 | None:intergenic |
| TCAATCCTTATCGTTGGCGT+AGG | 0.573889 | 4.1:+7138863 | MS.gene70112:CDS |
| CGATCTGAGAGAAGAGACCT+TGG | 0.574746 | 4.1:-7128677 | None:intergenic |
| TGCTGCTCCGATGTCGCCGG+TGG | 0.582433 | 4.1:+7128561 | MS.gene70112:CDS |
| ATGGAAAACATGATTTGTTG+GGG | 0.584941 | 4.1:+7134154 | MS.gene70112:CDS |
| AGAACAACAATTTCTTGGTG+AGG | 0.589436 | 4.1:+7131541 | MS.gene70112:CDS |
| GACAAGCGATTTCCTACGTG+GGG | 0.590517 | 4.1:+7135789 | MS.gene70112:CDS |
| AGATGCACTTGTTAAAGCAT+CGG | 0.592040 | 4.1:+7138829 | MS.gene70112:CDS |
| CAGTTCCGCCGATTGCCGTA+CGG | 0.592626 | 4.1:-7128584 | None:intergenic |
| TACATGACTAGAGATTTGGT+TGG | 0.595044 | 4.1:-7139301 | None:intergenic |
| AATTCAAAACTACTTCCGTC+CGG | 0.595748 | 4.1:-7130433 | None:intergenic |
| CTACTAGGATGGAGTTGTGA+CGG | 0.599974 | 4.1:+7138790 | MS.gene70112:intron |
| TGTTCAACAAGTAGGAAGCA+AGG | 0.601240 | 4.1:+7132277 | MS.gene70112:CDS |
| TTCAAGTCTCGTGGCTACCA+AGG | 0.609125 | 4.1:+7128660 | MS.gene70112:CDS |
| GAGCACTTGAGGAACTTGGC+CGG | 0.609390 | 4.1:+7130414 | MS.gene70112:CDS |
| ACTTGAGGAACTTGGCCGGA+CGG | 0.609426 | 4.1:+7130418 | MS.gene70112:CDS |
| AGCTGATTTCAAAGAGATGG+AGG | 0.615204 | 4.1:+7138889 | MS.gene70112:CDS |
| TGAATTCGTTGAATCCGACA+TGG | 0.617366 | 4.1:+7130450 | MS.gene70112:CDS |
| CAGACAAGCGATTTCCTACG+TGG | 0.619290 | 4.1:+7135787 | MS.gene70112:CDS |
| CAGATCTTGGATGCAGACAA+AGG | 0.620101 | 4.1:+7139041 | MS.gene70112:intron |
| GTTTCACAACGCAGACGTGA+AGG | 0.620590 | 4.1:+7130641 | MS.gene70112:CDS |
| AAAACTGCAGACATCTCTGG+TGG | 0.621038 | 4.1:+7134262 | MS.gene70112:CDS |
| TTGTAGCACTCTATTAACAA+AGG | 0.621510 | 4.1:-7134120 | None:intergenic |
| GTTTGTTTAGTAATCCATGT+CGG | 0.622631 | 4.1:-7130464 | None:intergenic |
| CTACAGTAGAGATGATACTC+AGG | 0.624513 | 4.1:+7132014 | MS.gene70112:CDS |
| CGATAAGGATTGATAATGGT+AGG | 0.628743 | 4.1:-7138853 | None:intergenic |
| AGAATGATCACAATCCAACA+TGG | 0.632845 | 4.1:+7132237 | MS.gene70112:CDS |
| GGTGGCCGTACGGCAATCGG+CGG | 0.635146 | 4.1:+7128579 | MS.gene70112:CDS |
| TGTAATGCAAAGAATCTGGA+AGG | 0.637807 | 4.1:-7135616 | None:intergenic |
| CTGGAGAAGCTCTATTCTGG+TGG | 0.643477 | 4.1:+7134287 | MS.gene70112:CDS |
| AGGCATTCAAGGAATCATGA+TGG | 0.645806 | 4.1:+7135968 | MS.gene70112:CDS |
| GATAACTTCTTCAAGTCTCG+TGG | 0.649983 | 4.1:+7128651 | MS.gene70112:CDS |
| AGCCAATCTGTAGCAAATGG+TGG | 0.650165 | 4.1:+7136074 | MS.gene70112:CDS |
| GGTCTGAGAATCATGACCCA+CGG | 0.650774 | 4.1:-7134339 | None:intergenic |
| AGGTCTCTTCTCTCAGATCG+AGG | 0.653280 | 4.1:+7128680 | MS.gene70112:CDS |
| GGCAACTTGTGCATTATCTG+AGG | 0.654797 | 4.1:+7131562 | MS.gene70112:CDS |
| AGACAAGCGATTTCCTACGT+GGG | 0.662700 | 4.1:+7135788 | MS.gene70112:CDS |
| GCAATCGGCGGAACTGCCGG+AGG | 0.665003 | 4.1:+7128591 | MS.gene70112:CDS |
| ATTACATTGATCCTTCAGGA+CGG | 0.667479 | 4.1:+7135633 | MS.gene70112:CDS |
| ATTTGAATTGAACTTCATGG+TGG | 0.674358 | 4.1:+7134524 | MS.gene70112:CDS |
| GTGTATGCTGAGGAATGTGT+TGG | 0.678518 | 4.1:+7131983 | MS.gene70112:CDS |
| AGACTTGAAGAAGTTATCGA+CGG | 0.701794 | 4.1:-7128646 | None:intergenic |
| GAAGCTCAAAGTGTATGCTG+AGG | 0.705846 | 4.1:+7131973 | MS.gene70112:CDS |
| CGTGCTCCAAATCCCCACGT+AGG | 0.721272 | 4.1:-7135801 | None:intergenic |
| TGGAAGCAATACTTACTGTG+AGG | 0.795076 | 4.1:+7135863 | MS.gene70112:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 7128552 | 7139316 | 7128552 | ID=MS.gene70112 |
| chr4.1 | mRNA | 7128552 | 7139316 | 7128552 | ID=MS.gene70112.t1;Parent=MS.gene70112 |
| chr4.1 | exon | 7128552 | 7128701 | 7128552 | ID=MS.gene70112.t1.exon1;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7128552 | 7128701 | 7128552 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7128790 | 7128837 | 7128790 | ID=MS.gene70112.t1.exon2;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7128790 | 7128837 | 7128790 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7130374 | 7130522 | 7130374 | ID=MS.gene70112.t1.exon3;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7130374 | 7130522 | 7130374 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7130614 | 7130662 | 7130614 | ID=MS.gene70112.t1.exon4;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7130614 | 7130662 | 7130614 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7131527 | 7131583 | 7131527 | ID=MS.gene70112.t1.exon5;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7131527 | 7131583 | 7131527 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7131890 | 7132075 | 7131890 | ID=MS.gene70112.t1.exon6;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7131890 | 7132075 | 7131890 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7132164 | 7132298 | 7132164 | ID=MS.gene70112.t1.exon7;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7132164 | 7132298 | 7132164 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7134114 | 7134175 | 7134114 | ID=MS.gene70112.t1.exon8;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7134114 | 7134175 | 7134114 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7134262 | 7134364 | 7134262 | ID=MS.gene70112.t1.exon9;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7134262 | 7134364 | 7134262 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7134447 | 7134561 | 7134447 | ID=MS.gene70112.t1.exon10;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7134447 | 7134561 | 7134447 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7135597 | 7135673 | 7135597 | ID=MS.gene70112.t1.exon11;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7135597 | 7135673 | 7135597 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7135747 | 7135884 | 7135747 | ID=MS.gene70112.t1.exon12;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7135747 | 7135884 | 7135747 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7135963 | 7136127 | 7135963 | ID=MS.gene70112.t1.exon13;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7135963 | 7136127 | 7135963 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7138797 | 7138910 | 7138797 | ID=MS.gene70112.t1.exon14;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7138797 | 7138910 | 7138797 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7139044 | 7139140 | 7139044 | ID=MS.gene70112.t1.exon15;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7139044 | 7139140 | 7139044 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| chr4.1 | exon | 7139222 | 7139316 | 7139222 | ID=MS.gene70112.t1.exon16;Parent=MS.gene70112.t1 |
| chr4.1 | CDS | 7139222 | 7139316 | 7139222 | ID=cds.MS.gene70112.t1;Parent=MS.gene70112.t1 |
| Gene Sequence |
| Protein sequence |
