AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50963


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50963.t1 MTR_3g037750 47.126 87 44 2 1 86 1 86 3.76e-16 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 23 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCTTGTTTGTGTTCTGTTTC+TGG 0.051760 3.2:-27080211 MS.gene50963:CDS
TTTCAATTTCAAGGGCATTT+TGG 0.264806 3.2:+27078190 None:intergenic
GGTCTTCCAATTGTTGTAAC+AGG 0.291635 3.2:+27078225 None:intergenic
CATGACTTGTTTCAATTTCA+AGG 0.329598 3.2:+27078181 None:intergenic
TGTGTTCCTCCTGGCTATTA+TGG 0.372312 3.2:-27078265 MS.gene50963:CDS
TGTGTCCCTGTTACAACAAT+TGG 0.425313 3.2:-27078231 MS.gene50963:CDS
GCAAAAGATGCTGCTCAGTA+TGG 0.425821 3.2:-27080015 MS.gene50963:CDS
CATCTTCTTGTGCATTGAGA+TGG 0.446525 3.2:+27078352 None:intergenic
ATCTTCTTGTGCATTGAGAT+GGG 0.464024 3.2:+27078353 None:intergenic
AAATGTCTCTGTGTTCCTCC+TGG 0.478257 3.2:-27078274 MS.gene50963:CDS
AGGAGGAACACAGAGACATT+TGG 0.480481 3.2:+27078276 None:intergenic
ATGACTTGTTTCAATTTCAA+GGG 0.510609 3.2:+27078182 None:intergenic
ACTAACCTGAGTGGTGATCA+TGG 0.521483 3.2:+27080173 None:intergenic
GATTTGTTACCATAATAGCC+AGG 0.572427 3.2:+27078256 None:intergenic
GTCTTCCAATTGTTGTAACA+GGG 0.578624 3.2:+27078226 None:intergenic
TCTCAATGCACAAGAAGATG+TGG 0.608334 3.2:-27078349 MS.gene50963:CDS
CATTGCCATGATCACCACTC+AGG 0.609926 3.2:-27080178 MS.gene50963:intron
AACAATTGGAAGACCAAGCG+TGG 0.618322 3.2:-27078217 MS.gene50963:CDS
AACACAAACAAGCTTAGCCA+TGG 0.629211 3.2:+27080221 None:intergenic
TGGTGATCATGGCAATGCTA+AGG 0.649208 3.2:+27080184 None:intergenic
AATTGGAAGACCAAGCGTGG+AGG 0.716723 3.2:-27078214 MS.gene50963:CDS
TTGTTACCATAATAGCCAGG+AGG 0.723243 3.2:+27078259 None:intergenic
TAGGAGGAGACTAACCTGAG+TGG 0.729040 3.2:+27080164 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTATAATTAAATACAG+AGG - chr3.2:27078913-27078932 MS.gene50963:intron 10.0%
!! TAAAAACTTAGTAAAAAAAA+GGG - chr3.2:27079260-27079279 MS.gene50963:intron 10.0%
!!! ATTATTCTTTTATTAAGATT+AGG + chr3.2:27078321-27078340 None:intergenic 10.0%
!!! TTAATTTTGTACATAAAATA+TGG - chr3.2:27078611-27078630 MS.gene50963:intron 10.0%
!! AAAAAAAGTCTATGTTAAAA+TGG + chr3.2:27079020-27079039 None:intergenic 15.0%
!! AAAAACTTAGTAAAAAAAAG+GGG - chr3.2:27079261-27079280 MS.gene50963:intron 15.0%
!! GTAAAAACTTAGTAAAAAAA+AGG - chr3.2:27079259-27079278 MS.gene50963:intron 15.0%
!! TTAAATAAAGAATATTTCGT+GGG - chr3.2:27079930-27079949 MS.gene50963:intron 15.0%
!! TTTAAATAAAGAATATTTCG+TGG - chr3.2:27079929-27079948 MS.gene50963:intron 15.0%
!!! AAAAAGTTGGTATATTTATA+GGG - chr3.2:27079725-27079744 MS.gene50963:intron 15.0%
!!! AATTTTTTCGATTTATTTAC+AGG - chr3.2:27078513-27078532 MS.gene50963:intron 15.0%
!!! AGTATCATTAGTTAATTTTT+AGG + chr3.2:27079148-27079167 None:intergenic 15.0%
!!! ATATATCCATTTTAAATTAC+TGG + chr3.2:27079779-27079798 None:intergenic 15.0%
!!! ATGAATTTTTATATTTTTGG+TGG + chr3.2:27079509-27079528 None:intergenic 15.0%
!!! ATTTTTTCGATTTATTTACA+GGG - chr3.2:27078514-27078533 MS.gene50963:intron 15.0%
!!! TAGTGATTGAAAATTTTATA+GGG + chr3.2:27079364-27079383 None:intergenic 15.0%
!!! TTTTATGTACAAAATTAAGT+TGG + chr3.2:27078609-27078628 None:intergenic 15.0%
!!! TTTTTTACTAAGTTTTTACT+AGG + chr3.2:27079258-27079277 None:intergenic 15.0%
!! AAATCACCAGTAATTTAAAA+TGG - chr3.2:27079770-27079789 MS.gene50963:intron 20.0%
!! CACATAAACAAATATATAAG+TGG + chr3.2:27079189-27079208 None:intergenic 20.0%
!! CACTTATATATTTGTTTATG+TGG - chr3.2:27079187-27079206 MS.gene50963:intron 20.0%
!! TTATTAAGATTAGGAAGATT+AGG + chr3.2:27078312-27078331 None:intergenic 20.0%
!! TTCAGAAACATATAATAAGT+GGG + chr3.2:27078815-27078834 None:intergenic 20.0%
!! TTTCAGAAACATATAATAAG+TGG + chr3.2:27078816-27078835 None:intergenic 20.0%
!!! ACATGCTTTTTTTTCTTTTA+TGG - chr3.2:27078703-27078722 MS.gene50963:intron 20.0%
!!! ATATGAGTTTACTTTAAAAG+AGG - chr3.2:27079633-27079652 MS.gene50963:intron 20.0%
!!! ATCATTAGTTAATTTTTAGG+CGG + chr3.2:27079145-27079164 None:intergenic 20.0%
!!! CAAAAAGTTGGTATATTTAT+AGG - chr3.2:27079724-27079743 MS.gene50963:intron 20.0%
!!! GAAAAGTTGGTATATTTATT+GGG + chr3.2:27079304-27079323 None:intergenic 20.0%
!!! GTAGTGATTGAAAATTTTAT+AGG + chr3.2:27079365-27079384 None:intergenic 20.0%
!!! TAAATTACTGGTGATTTTTA+GGG + chr3.2:27079767-27079786 None:intergenic 20.0%
!!! TATGAGTTTACTTTAAAAGA+GGG - chr3.2:27079634-27079653 MS.gene50963:intron 20.0%
!!! TATGTGGCAATTTTATTTTT+TGG - chr3.2:27079203-27079222 MS.gene50963:intron 20.0%
!!! TTAAATTACTGGTGATTTTT+AGG + chr3.2:27079768-27079787 None:intergenic 20.0%
!!! TTTTAGGGTTAATATGTTTT+TGG + chr3.2:27079752-27079771 None:intergenic 20.0%
!!! TTTTTTCGATTTATTTACAG+GGG - chr3.2:27078515-27078534 MS.gene50963:intron 20.0%
! AAATGAATGAATGTTAGAGA+CGG + chr3.2:27078955-27078974 None:intergenic 25.0%
! ATTTATTTACAGGGGAATTA+TGG - chr3.2:27078523-27078542 MS.gene50963:intron 25.0%
! CATGAGGAAATTTCTTTAAA+TGG + chr3.2:27078884-27078903 None:intergenic 25.0%
! TAAAGAAATTTCCTCATGTA+TGG - chr3.2:27078886-27078905 MS.gene50963:intron 25.0%
! TCAGAAACATATAATAAGTG+GGG + chr3.2:27078814-27078833 None:intergenic 25.0%
!! AAATTTCCTATACATTTTGC+AGG - chr3.2:27078349-27078368 MS.gene50963:CDS 25.0%
!! AAGTTGAAGGAAAATTTTAG+AGG + chr3.2:27079712-27079693 None:intergenic 25.0%
!! AGTTGAAGGAAAATTTTAGA+GGG + chr3.2:27079713-27079694 None:intergenic 25.0%
!! TAATTTTTGTACCATACATG+AGG + chr3.2:27078900-27078919 None:intergenic 25.0%
!! TTTTGCAAAATGCCTATTAA+AGG + chr3.2:27078457-27078476 None:intergenic 25.0%
!!! ATGACTTGTTTCAATTTCAA+GGG + chr3.2:27080213-27080232 None:intergenic 25.0%
!!! ATTAAGTTGGTCTACAAAAA+TGG + chr3.2:27078596-27078615 None:intergenic 25.0%
!!! CGAAAAGTTGGTATATTTAT+TGG + chr3.2:27079305-27079324 None:intergenic 25.0%
!!! TGAAGATGGTTAGTTTTAAT+GGG - chr3.2:27079665-27079684 MS.gene50963:intron 25.0%
AAATATTAAGCAATGCTCTG+AGG - chr3.2:27079068-27079087 MS.gene50963:intron 30.0%
AAATGCCTATTAAAGGAACT+AGG + chr3.2:27078450-27078469 None:intergenic 30.0%
AATATTAAGCAATGCTCTGA+GGG - chr3.2:27079069-27079088 MS.gene50963:intron 30.0%
AATTCTCAAAACAGAGAGAA+AGG + chr3.2:27078415-27078434 None:intergenic 30.0%
GAGAGACTAAAACAAAAAGT+TGG - chr3.2:27079712-27079731 MS.gene50963:intron 30.0%
GAGCATTCAAACAGAAATAT+AGG + chr3.2:27079810-27079829 None:intergenic 30.0%
TCTTTCCTAGTTCCTTTAAT+AGG - chr3.2:27078442-27078461 MS.gene50963:intron 30.0%
TGATACACATTCATGTAACA+TGG + chr3.2:27078272-27078291 None:intergenic 30.0%
TTAATGGGACTAAAAGTTGA+AGG - chr3.2:27079680-27079699 MS.gene50963:intron 30.0%
TTATAGGGACTAAAAGTTGA+AGG + chr3.2:27079349-27079368 None:intergenic 30.0%
! AAACTAACCATCTTCACTTT+TGG + chr3.2:27079661-27079680 None:intergenic 30.0%
! ATGGATATATGATTGACTCA+CGG + chr3.2:27078570-27078589 None:intergenic 30.0%
!! CATGACTTGTTTCAATTTCA+AGG + chr3.2:27080214-27080233 None:intergenic 30.0%
!! GTTTTGTGTTGAGTTGTTTT+AGG + chr3.2:27078250-27078269 None:intergenic 30.0%
!!! GTGAAGATGGTTAGTTTTAA+TGG - chr3.2:27079664-27079683 MS.gene50963:intron 30.0%
!!! GTGGCAATTTTATTTTTTGG+TGG - chr3.2:27079206-27079225 MS.gene50963:intron 30.0%
!!! TTTCAATTTCAAGGGCATTT+TGG + chr3.2:27080205-27080224 None:intergenic 30.0%
ACTAGGTACTTTGACGAATA+TGG + chr3.2:27079241-27079260 None:intergenic 35.0%
ATCTTCTTGTGCATTGAGAT+GGG + chr3.2:27080042-27080061 None:intergenic 35.0%
CCATAACCTGCAAAATGTAT+AGG + chr3.2:27078358-27078377 None:intergenic 35.0%
CTAGGTACTTTGACGAATAT+GGG + chr3.2:27079240-27079259 None:intergenic 35.0%
GTCTTCCAATTGTTGTAACA+GGG + chr3.2:27080169-27080188 None:intergenic 35.0%
GTTAACACAATGCAGTCTAA+TGG - chr3.2:27079983-27080002 MS.gene50963:intron 35.0%
TAAAGGAACTAGGAAAGAAG+TGG + chr3.2:27078440-27078459 None:intergenic 35.0%
TCTGAGAGAAATTGAATGAG+TGG - chr3.2:27079857-27079876 MS.gene50963:intron 35.0%
TGAATGAGTGGAATCAAACA+TGG - chr3.2:27079869-27079888 MS.gene50963:intron 35.0%
TGGTCTACAAAAATGGAGTA+TGG + chr3.2:27078589-27078608 None:intergenic 35.0%
! CCTATACATTTTGCAGGTTA+TGG - chr3.2:27078355-27078374 MS.gene50963:CDS 35.0%
! TTTTGACAGAAGAACATGCA+TGG + chr3.2:27080088-27080107 None:intergenic 35.0%
!! GATTTGTTACCATAATAGCC+AGG + chr3.2:27080139-27080158 None:intergenic 35.0%
!!! TTGTGTTGAGTTGTTTTAGG+AGG + chr3.2:27078247-27078266 None:intergenic 35.0%
CATCTTCTTGTGCATTGAGA+TGG + chr3.2:27080043-27080062 None:intergenic 40.0%
GCTTGTTTGTGTTCTGTTTC+TGG - chr3.2:27078181-27078200 MS.gene50963:CDS 40.0%
GGTCTTCCAATTGTTGTAAC+AGG + chr3.2:27080170-27080189 None:intergenic 40.0%
TCTCAATGCACAAGAAGATG+TGG - chr3.2:27080043-27080062 MS.gene50963:intron 40.0%
TGTGTCCCTGTTACAACAAT+TGG - chr3.2:27080161-27080180 MS.gene50963:intron 40.0%
TTGTTACCATAATAGCCAGG+AGG + chr3.2:27080136-27080155 None:intergenic 40.0%
! AGGCGGCTAATTTTCATTGA+TGG + chr3.2:27079128-27079147 None:intergenic 40.0%
AAATGTCTCTGTGTTCCTCC+TGG - chr3.2:27080118-27080137 MS.gene50963:intron 45.0%
AACAATTGGAAGACCAAGCG+TGG - chr3.2:27080175-27080194 MS.gene50963:intron 45.0%
ACTAACCTGAGTGGTGATCA+TGG + chr3.2:27078222-27078241 None:intergenic 45.0%
AGAGGGACCAAAAGTGAAGA+TGG - chr3.2:27079651-27079670 MS.gene50963:intron 45.0%
AGGAGGAACACAGAGACATT+TGG + chr3.2:27080119-27080138 None:intergenic 45.0%
GAGGGACGAAAACGAAAAGT+TGG + chr3.2:27079317-27079336 None:intergenic 45.0%
GCAAAAGATGCTGCTCAGTA+TGG - chr3.2:27078377-27078396 MS.gene50963:intron 45.0%
TACAGGGGAATTATGGACCT+GGG - chr3.2:27078530-27078549 MS.gene50963:intron 45.0%
TGGTGATCATGGCAATGCTA+AGG + chr3.2:27078211-27078230 None:intergenic 45.0%
TGTGTTCCTCCTGGCTATTA+TGG - chr3.2:27080127-27080146 MS.gene50963:intron 45.0%
TTACAGGGGAATTATGGACC+TGG - chr3.2:27078529-27078548 MS.gene50963:intron 45.0%
!!! AAAAAATTCTAATTTTTTTT+GGG + chr3.2:27079567-27079548 None:intergenic 5.0%
!!! AAAAAATTCTAATTTTTTTT+TGG - chr3.2:27079548-27079567 MS.gene50963:intron 5.0%
!!! AAAAATTCTAATTTTTTTTT+GGG - chr3.2:27079549-27079568 MS.gene50963:intron 5.0%
!!! AATATGAATTTTTATATTTT+TGG + chr3.2:27079512-27079531 None:intergenic 5.0%
!!! TAAAAAATTCTAATTTTTTT+TGG + chr3.2:27079566-27079547 None:intergenic 5.0%
AATTGGAAGACCAAGCGTGG+AGG - chr3.2:27080178-27080197 MS.gene50963:intron 50.0%
CATTGCCATGATCACCACTC+AGG - chr3.2:27078214-27078233 MS.gene50963:CDS 50.0%
TAGGAGGAGACTAACCTGAG+TGG + chr3.2:27078231-27078250 None:intergenic 50.0%
!! CGGTAGCTTTGAAGACTCCC+AGG + chr3.2:27078550-27078569 None:intergenic 55.0%
!! GCATTTTGGTCCTCCACGCT+TGG + chr3.2:27080191-27080210 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 27078173 27080241 27078173 ID=MS.gene50963
chr3.2 mRNA 27078173 27080241 27078173 ID=MS.gene50963.t1;Parent=MS.gene50963
chr3.2 exon 27080179 27080241 27080179 ID=MS.gene50963.t1.exon1;Parent=MS.gene50963.t1
chr3.2 CDS 27080179 27080241 27080179 ID=cds.MS.gene50963.t1;Parent=MS.gene50963.t1
chr3.2 exon 27080011 27080043 27080011 ID=MS.gene50963.t1.exon2;Parent=MS.gene50963.t1
chr3.2 CDS 27080011 27080043 27080011 ID=cds.MS.gene50963.t1;Parent=MS.gene50963.t1
chr3.2 exon 27078173 27078379 27078173 ID=MS.gene50963.t1.exon3;Parent=MS.gene50963.t1
chr3.2 CDS 27078173 27078379 27078173 ID=cds.MS.gene50963.t1;Parent=MS.gene50963.t1
Gene Sequence

>MS.gene50963

ATGGCTAAGCTTGTTTGTGTTCTGTTTCTGGCACTCCTTAGCATTGCCATGATCACCACTCAGGTTAGTCTCCTCCTAAAACAACTCAACACAAAACCATGTTACATGAATGTGTATCATTGTTGTTTTATGATATCCTAATCTTCCTAATCTTAATAAAAGAATAATCTTGCTAAAAATTTCCTATACATTTTGCAGGTTATGGCAAAAGATGCTGCTCAGTATGGCAGTGTGAGTATCCTTTCTCTCTGTTTTGAGAATTTTCCACTTCTTTCCTAGTTCCTTTAATAGGCATTTTGCAAAAGTTTCATAAATTTTACTTAAAATTAGTTTGTTTACTAATTTTTTCGATTTATTTACAGGGGAATTATGGACCTGGGAGTCTTCAAAGCTACCGTGAGTCAATCATATATCCATACTCCATTTTTGTAGACCAACTTAATTTTGTACATAAAATATGGCTTTTCAAGTTGCATCATGCACTCTTAAAGTTTTAACTCATTAATTTTCTTTAATTATGAGACAGTTAAACATGCTTTTTTTTCTTTTATGGATTACAGTTAAACATACTTTTAATATTTGTAAAATTTCGTTGATTTAAATTTATTTTGTACTTAATTTTTCTTATAAGTTTCACACCCCACTTATTATATGTTTCTGAAATCTCTCGTAAAATAGTCATTTTAGTACTTAAATATATGAATGATGCCATTTAAAGAAATTTCCTCATGTATGGTACAAAAATTATAATTAAATACAGAGGAATGTTATGAATAACACCGTCTCTAACATTCATTCATTTATTAGATGAAACACATATGAATTTCATTTCTTTTTATATTGTCCATTTTAACATAGACTTTTTTTATCTTGTACTCTTTTTAAATATGTGAGAAAATATTAAGCAATGCTCTGAGGGCACTGTTTAACATTCTCGAAAAAAAAAAAATATCCATCAATGAAAATTAGCCGCCTAAAAATTAACTAATGATACTTTTAAACTAATTTTGACTCCACTTATATATTTGTTTATGTGGCAATTTTATTTTTTGGTGGATAAATTACCCATATTCGTCAAAGTACCTAGTAAAAACTTAGTAAAAAAAAGGGGTTAAATATGTTTTTGATCCCAATAAATATACCAACTTTTCGTTTTCGTCCCTCTAAAATTTTCCTTCAACTTTTAGTCCCTATAAAATTTTCAATCACTACTTTTAAGTTAATTTTTGTATTTTTTAATTAAATTGTGCAGAAATGTGTAGATATTGTAAAAAGAAATCCCAAAAAAAATTAGAATTTTTTAACAAAACATAAATTAATTATGAATTTTTAACCACCAAAAATATAAAAATTCATATTAAATTCATGTTTTGTTAAAAAATTCTAATTTTTTTTTGGGAGAGATTTTTATAGTATTATGAATTTTTGTGCAAAATTTTATTCAATAATATGAATTTTACATATGAGTTTACTTTAAAAGAGGGACCAAAAGTGAAGATGGTTAGTTTTAATGGGACTAAAAGTTGAAGGAAAATTTTAGAGAGACTAAAACAAAAAGTTGGTATATTTATAGGGACCAAAAACATATTAACCCTAAAAATCACCAGTAATTTAAAATGGATATATATGTTTGACCTATATTTCTGTTTGAATGCTCAACAAAAAAATAAGTTAGAATAATCAATCTGAGAGAAATTGAATGAGTGGAATCAAACATGGCACAAAACTTAGTTGAGTTGAGACTAGATCAACTAAATTTAAATAAAGAATATTTCGTGGGAAAAAAAAACACACTTTAACTGATTATCAAGTTAACACAATGCAGTCTAATGGATTTACGTTTGTGAAATTGATTTGATCAGAGTGCCCATCTCAATGCACAAGAAGATGTGGACAGACACAATATCACAAGCCATGCATGTTCTTCTGTCAAAAATGTTGTGCCAAATGTCTCTGTGTTCCTCCTGGCTATTATGGTAACAAATCTGTGTGTCCCTGTTACAACAATTGGAAGACCAAGCGTGGAGGACCAAAATGCCCTTGAAATTGAAACAAGTCATGATCATTAA

Protein sequence

>MS.gene50963.t1

MAKLVCVLFLALLSIAMITTQVMAKDAAQYGSSAHLNAQEDVDRHNITSHACSSVKNVVPNVSVFLLAIMVTNLCVPVTTIGRPSVEDQNALEIETSHDH