AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene004991

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004991.t1 MTR_1g078200 99.107 224 2 0 1 224 1 224 2.44e-167 460
MS.gene004991.t1 MTR_7g405790 64.651 215 64 2 13 223 14 220 9.00e-96 279
MS.gene004991.t1 MTR_7g051940 73.889 180 47 0 13 192 12 191 8.16e-93 271
MS.gene004991.t1 MTR_4g064897 59.906 212 78 3 12 223 10 214 6.23e-88 259
MS.gene004991.t1 MTR_8g089985 62.887 194 72 0 12 205 15 208 1.20e-86 256
MS.gene004991.t1 MTR_3g086980 57.820 211 86 1 12 222 14 221 3.55e-85 252
MS.gene004991.t1 MTR_0262s0050 55.450 211 90 1 12 222 13 219 2.84e-82 244
MS.gene004991.t1 MTR_2g005510 55.556 216 91 2 8 223 9 219 3.50e-82 244
MS.gene004991.t1 MTR_1g052475 55.660 212 91 3 12 222 13 222 7.20e-82 244
MS.gene004991.t1 MTR_3g069510 55.605 223 91 4 1 223 1 215 1.24e-81 243
MS.gene004991.t1 MTR_4g079350 57.075 212 85 3 12 223 11 216 1.71e-80 240
MS.gene004991.t1 MTR_2g014990 56.604 212 86 3 12 223 11 216 4.37e-80 239
MS.gene004991.t1 MTR_2g083860 63.006 173 64 0 12 184 11 183 2.59e-79 237
MS.gene004991.t1 MTR_4g088090 55.189 212 89 3 12 223 11 216 3.80e-79 236
MS.gene004991.t1 MTR_2g075950 54.245 212 91 2 12 223 11 216 2.02e-78 234
MS.gene004991.t1 MTR_2g090865 55.660 212 87 2 12 223 9 213 9.64e-77 230
MS.gene004991.t1 MTR_8g036220 51.869 214 101 1 12 223 14 227 1.66e-76 230
MS.gene004991.t1 MTR_8g027220 59.239 184 74 1 7 190 20 202 2.32e-76 230
MS.gene004991.t1 MTR_6g079550 52.582 213 93 3 12 223 10 215 1.08e-68 210
MS.gene004991.t1 MTR_8g107070 54.315 197 89 1 12 208 12 207 1.00e-65 204
MS.gene004991.t1 MTR_7g117910 62.238 143 54 0 12 154 18 160 1.07e-64 199
MS.gene004991.t1 MTR_7g081700 45.283 212 100 3 12 223 6 201 5.97e-57 179
MS.gene004991.t1 MTR_2g027660 43.602 211 115 1 13 223 5 211 2.19e-56 178
MS.gene004991.t1 MTR_2g005500 51.807 166 80 0 12 177 7 172 3.55e-56 177
MS.gene004991.t1 MTR_1g068740 42.453 212 111 2 12 222 13 214 1.29e-55 177
MS.gene004991.t1 MTR_1g068730 42.453 212 111 2 12 222 13 214 1.42e-55 176
MS.gene004991.t1 MTR_8g006970 44.811 212 102 3 12 223 20 216 1.51e-55 176
MS.gene004991.t1 MTR_1g115325 42.326 215 107 4 12 222 13 214 1.02e-54 174
MS.gene004991.t1 MTR_6g021790 44.131 213 113 2 12 223 4 211 1.07e-54 174
MS.gene004991.t1 MTR_7g100020 49.697 165 82 1 12 175 13 177 2.29e-54 173
MS.gene004991.t1 MTR_8g006970 51.220 164 80 0 12 175 6 169 2.70e-54 172
MS.gene004991.t1 MTR_8g031660 49.701 167 84 0 10 176 2 168 4.43e-54 172
MS.gene004991.t1 MTR_2g096550 47.059 170 88 2 8 175 9 178 5.67e-52 167
MS.gene004991.t1 MTR_8g031870 50.943 159 78 0 10 168 2 160 6.53e-52 166
MS.gene004991.t1 MTR_8g031680 41.121 214 121 3 10 223 2 210 3.97e-50 162
MS.gene004991.t1 MTR_4g063160 41.121 214 112 4 12 222 13 215 1.36e-49 161
MS.gene004991.t1 MTR_3g064390 40.385 208 105 2 16 223 12 200 1.68e-48 158
MS.gene004991.t1 MTR_8g031830 49.342 152 77 0 13 164 5 156 1.90e-47 155
MS.gene004991.t1 MTR_5g076590 38.942 208 108 3 16 223 12 200 6.36e-47 154
MS.gene004991.t1 MTR_8g093740 43.716 183 100 3 12 192 11 192 2.38e-45 150
MS.gene004991.t1 MTR_4g099470 41.176 187 94 2 37 223 7 177 1.92e-44 147
MS.gene004991.t1 MTR_1g068730 50.769 130 64 0 12 141 13 142 1.97e-44 147
MS.gene004991.t1 MTR_1g094220 44.099 161 89 1 16 175 33 193 2.07e-44 147
MS.gene004991.t1 MTR_1g068740 50.769 130 64 0 12 141 13 142 2.43e-44 147
MS.gene004991.t1 MTR_1g094220 43.275 171 91 2 16 180 33 203 2.50e-44 147
MS.gene004991.t1 MTR_7g103070 40.187 214 113 5 12 223 14 214 4.46e-44 147
MS.gene004991.t1 MTR_3g096350 45.029 171 91 3 12 180 10 179 7.79e-44 146
MS.gene004991.t1 MTR_1g090717 40.183 219 105 5 12 223 13 212 1.43e-43 145
MS.gene004991.t1 MTR_1g029450 41.026 195 103 4 12 195 15 208 2.50e-43 145
MS.gene004991.t1 MTR_1g062760 41.014 217 104 6 12 222 12 210 4.09e-43 144
MS.gene004991.t1 MTR_1g094220 44.872 156 85 1 16 170 33 188 4.62e-43 144
MS.gene004991.t1 MTR_1g094220 45.161 155 84 1 16 169 33 187 1.26e-42 144
MS.gene004991.t1 MTR_2g102060 41.317 167 98 0 16 182 12 178 1.39e-42 143
MS.gene004991.t1 MTR_5g013900 39.490 157 95 0 15 171 10 166 1.65e-40 137
MS.gene004991.t1 MTR_4g098950 38.854 157 96 0 15 171 10 166 2.69e-40 137
MS.gene004991.t1 MTR_8g103550 40.625 160 95 0 16 175 11 170 3.20e-40 137
MS.gene004991.t1 MTR_3g087160 36.313 179 108 1 15 193 10 182 3.56e-40 137
MS.gene004991.t1 MTR_8g090215 38.217 157 97 0 15 171 10 166 1.66e-39 135
MS.gene004991.t1 MTR_3g096350 44.521 146 78 3 12 155 10 154 7.89e-37 127
MS.gene004991.t1 MTR_3g096350 50.413 121 57 3 12 130 10 129 1.31e-36 126
MS.gene004991.t1 MTR_3g064390 45.902 122 66 0 16 137 12 133 2.46e-35 123
MS.gene004991.t1 MTR_4g069850 36.310 168 100 3 14 174 8 175 4.57e-33 119
MS.gene004991.t1 MTR_8g092020 37.725 167 97 2 14 173 12 178 1.47e-32 117
MS.gene004991.t1 MTR_8g093740 41.333 150 86 2 45 192 10 159 6.25e-32 115
MS.gene004991.t1 MTR_1g097420 37.126 167 98 2 14 173 8 174 6.46e-32 115
MS.gene004991.t1 MTR_4g012940 35.928 167 100 3 14 173 8 174 2.89e-31 114
MS.gene004991.t1 MTR_2g101890 35.329 167 101 3 14 173 10 176 3.64e-31 114
MS.gene004991.t1 MTR_7g103070 38.235 170 90 5 56 223 2 158 3.38e-30 110
MS.gene004991.t1 MTR_6g039490 33.014 209 124 5 14 206 8 216 1.23e-29 110
MS.gene004991.t1 MTR_2g005500 38.554 166 62 1 12 177 7 132 9.25e-28 103
MS.gene004991.t1 MTR_1g097420 34.911 169 101 3 12 173 35 201 1.23e-26 102
MS.gene004991.t1 MTR_8g031810 41.346 104 47 1 13 116 5 94 2.63e-21 85.1
MS.gene004991.t1 MTR_3g107717 30.114 176 117 5 1 175 1 171 1.40e-20 86.7
MS.gene004991.t1 MTR_3g107713 30.114 176 117 5 1 175 1 171 1.52e-20 86.7
MS.gene004991.t1 MTR_3g107707 30.114 176 117 5 1 175 1 171 1.67e-20 86.3
MS.gene004991.t1 MTR_1g069245 47.368 95 46 2 27 121 109 199 1.66e-19 83.6
MS.gene004991.t1 MTR_1452s0010 41.772 79 46 0 34 112 1 79 1.80e-18 77.8
MS.gene004991.t1 MTR_2g090875 30.460 174 107 5 14 175 6 177 3.80e-18 79.3
MS.gene004991.t1 MTR_4g088055 30.233 172 106 5 14 173 6 175 4.13e-18 79.3
MS.gene004991.t1 MTR_4g073250 30.460 174 107 5 14 175 6 177 5.82e-18 79.0
MS.gene004991.t1 MTR_6g087980 28.421 190 122 5 14 191 6 193 3.40e-17 77.4
MS.gene004991.t1 MTR_2g078000 61.224 49 19 0 75 123 46 94 5.82e-17 73.9
MS.gene004991.t1 MTR_8g075240 30.114 176 105 6 14 175 6 177 7.86e-17 75.9
MS.gene004991.t1 MTR_5g022600 30.114 176 105 6 14 175 6 177 1.30e-16 75.5
MS.gene004991.t1 MTR_3g078260 28.652 178 113 6 10 175 4 179 6.68e-16 73.9
MS.gene004991.t1 MTR_1686s0010 37.975 79 49 0 34 112 1 79 1.66e-15 69.7
MS.gene004991.t1 MTR_1g059590 32.370 173 107 4 10 182 394 556 3.80e-15 74.3
MS.gene004991.t1 MTR_1g059590 32.370 173 107 4 10 182 427 589 4.52e-15 73.9
MS.gene004991.t1 MTR_4g113620 27.439 164 101 5 15 175 14 162 1.24e-14 70.5
MS.gene004991.t1 MTR_5g092060 24.862 181 129 3 4 178 93 272 9.60e-14 68.9
MS.gene004991.t1 MTR_2g078560 47.619 63 27 1 76 132 54 116 3.33e-13 67.8
MS.gene004991.t1 MTR_8g099250 60.465 43 17 0 12 54 14 56 9.71e-11 56.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene004991.t1 AT5G47520 85.268 224 28 2 1 223 1 220 1.51e-138 387
MS.gene004991.t1 AT2G43130 60.811 222 79 2 3 224 1 214 2.56e-95 277
MS.gene004991.t1 AT2G31680 62.443 221 79 1 3 223 1 217 1.12e-94 276
MS.gene004991.t1 AT1G05810 61.712 222 80 2 2 223 46 262 1.70e-93 275
MS.gene004991.t1 AT1G05810 61.538 221 80 2 3 223 1 216 2.94e-93 272
MS.gene004991.t1 AT3G07410 63.470 219 75 2 6 224 4 217 4.59e-93 272
MS.gene004991.t1 AT5G47960 58.685 213 81 2 12 222 13 220 1.01e-86 256
MS.gene004991.t1 AT5G65270 66.667 177 59 0 12 188 15 191 2.42e-86 255
MS.gene004991.t1 AT1G07410 57.547 212 82 3 12 223 10 213 1.01e-84 251
MS.gene004991.t1 AT5G59150 57.870 216 86 2 8 223 6 216 1.50e-84 250
MS.gene004991.t1 AT4G39990 57.277 213 85 3 12 223 15 222 1.61e-84 250
MS.gene004991.t1 AT3G12160 55.399 213 91 1 12 224 13 221 2.96e-84 249
MS.gene004991.t1 AT3G46830 57.075 212 86 1 12 223 10 216 8.84e-83 246
MS.gene004991.t1 AT3G46830 57.075 212 86 1 12 223 10 216 8.84e-83 246
MS.gene004991.t1 AT1G09630 53.812 223 95 3 1 223 1 215 2.85e-82 244
MS.gene004991.t1 AT4G18430 55.660 212 88 2 12 223 11 216 2.34e-81 242
MS.gene004991.t1 AT5G60860 57.075 212 85 2 12 223 11 216 4.18e-81 241
MS.gene004991.t1 AT1G16920 57.547 212 83 3 12 223 11 215 7.46e-81 241
MS.gene004991.t1 AT4G18800 55.660 212 85 2 12 223 11 213 3.41e-80 239
MS.gene004991.t1 AT1G28550 53.704 216 95 2 8 223 7 217 5.18e-78 234
MS.gene004991.t1 AT3G15060 54.930 213 88 3 12 223 11 216 2.97e-77 232
MS.gene004991.t1 AT1G06400 54.245 212 90 2 12 223 11 215 3.24e-77 231
MS.gene004991.t1 AT5G45750 53.302 212 92 2 12 223 11 215 1.18e-76 230
MS.gene004991.t1 AT2G33870 52.778 216 97 2 8 223 7 217 3.99e-76 229
MS.gene004991.t1 AT1G18200 52.511 219 98 2 12 224 11 229 1.05e-75 228
MS.gene004991.t1 AT1G73640 58.470 183 75 1 1 183 1 182 4.23e-73 222
MS.gene004991.t1 AT1G01200 58.427 178 71 2 12 186 26 203 1.28e-68 211
MS.gene004991.t1 AT5G59150 54.762 168 71 2 56 223 3 165 3.62e-59 184
MS.gene004991.t1 AT1G02130 45.283 212 101 3 12 223 6 202 1.55e-58 184
MS.gene004991.t1 AT3G11730 44.601 213 105 3 12 224 6 205 2.94e-57 181
MS.gene004991.t1 AT4G17170 43.128 211 116 1 13 223 5 211 9.53e-56 177
MS.gene004991.t1 AT4G35860 43.662 213 114 2 12 223 4 211 2.32e-55 176
MS.gene004991.t1 AT5G03520 44.601 213 106 3 12 223 13 214 4.04e-55 175
MS.gene004991.t1 AT3G09900 43.256 215 108 4 12 223 13 216 2.69e-54 173
MS.gene004991.t1 AT3G46060 49.412 170 85 1 12 180 13 182 5.30e-54 172
MS.gene004991.t1 AT3G46060 49.412 170 85 1 12 180 13 182 5.30e-54 172
MS.gene004991.t1 AT3G46060 49.412 170 85 1 12 180 13 182 5.30e-54 172
MS.gene004991.t1 AT3G53610 42.523 214 108 4 12 222 13 214 5.97e-54 172
MS.gene004991.t1 AT3G53610 42.523 214 108 4 12 222 13 214 5.97e-54 172
MS.gene004991.t1 AT3G53610 42.523 214 108 4 12 222 13 214 5.97e-54 172
MS.gene004991.t1 AT5G59840 49.697 165 82 1 12 175 13 177 8.37e-54 172
MS.gene004991.t1 AT4G17160 44.762 210 106 3 13 222 5 204 8.67e-54 172
MS.gene004991.t1 AT4G17530 43.396 212 104 2 12 223 6 201 9.52e-54 171
MS.gene004991.t1 AT5G47200 43.662 213 102 3 12 223 6 201 1.11e-53 171
MS.gene004991.t1 AT4G19640 38.942 208 108 3 16 223 12 200 5.82e-48 157
MS.gene004991.t1 AT3G54840 45.963 161 86 1 16 175 35 195 1.00e-45 151
MS.gene004991.t1 AT3G54840 45.625 160 86 1 17 175 2 161 1.98e-45 149
MS.gene004991.t1 AT3G54840 47.097 155 81 1 16 169 35 189 3.12e-45 149
MS.gene004991.t1 AT5G45130 42.945 163 93 0 16 178 12 174 5.65e-45 149
MS.gene004991.t1 AT5G45130 42.857 161 92 0 18 178 22 182 1.32e-43 145
MS.gene004991.t1 AT5G03530 41.014 217 106 7 12 223 11 210 1.35e-43 145
MS.gene004991.t1 AT5G03530 41.014 217 106 7 12 223 11 210 1.35e-43 145
MS.gene004991.t1 AT1G43890 45.783 166 87 3 12 175 11 175 1.05e-42 144
MS.gene004991.t1 AT1G43890 45.783 166 87 3 12 175 11 175 1.05e-42 144
MS.gene004991.t1 AT1G43890 45.783 166 87 3 12 175 11 175 1.05e-42 144
MS.gene004991.t1 AT4G39890 40.506 158 93 1 15 171 10 167 4.81e-42 142
MS.gene004991.t1 AT5G03520 41.477 176 91 3 49 223 40 204 1.27e-41 140
MS.gene004991.t1 AT2G44610 39.490 157 95 0 15 171 10 166 1.15e-40 138
MS.gene004991.t1 AT5G10260 34.450 209 125 3 15 223 10 206 1.29e-40 138
MS.gene004991.t1 AT4G35860 42.169 166 90 2 58 222 4 164 1.50e-38 131
MS.gene004991.t1 AT2G22290 36.943 157 99 0 15 171 10 166 1.24e-37 130
MS.gene004991.t1 AT3G09910 37.788 217 107 6 12 223 11 204 2.36e-37 129
MS.gene004991.t1 AT3G09910 37.788 217 107 6 12 223 11 204 2.36e-37 129
MS.gene004991.t1 AT3G09910 37.788 217 107 6 12 223 11 204 2.36e-37 129
MS.gene004991.t1 AT4G19640 43.846 130 73 0 16 145 12 141 5.25e-37 127
MS.gene004991.t1 AT4G19640 43.511 131 74 0 16 146 12 142 1.91e-36 127
MS.gene004991.t1 AT3G09910 39.572 187 104 4 12 193 11 193 4.29e-36 126
MS.gene004991.t1 AT3G09910 39.572 187 104 4 12 193 11 193 4.29e-36 126
MS.gene004991.t1 AT1G22740 38.323 167 96 2 14 173 8 174 1.32e-34 122
MS.gene004991.t1 AT5G64990 35.714 154 99 0 15 168 8 161 2.38e-33 119
MS.gene004991.t1 AT1G52280 37.647 170 99 4 14 176 8 177 3.90e-33 119
MS.gene004991.t1 AT3G18820 37.500 168 98 4 14 174 8 175 1.66e-32 117
MS.gene004991.t1 AT3G16100 37.725 167 97 3 14 173 8 174 5.93e-32 115
MS.gene004991.t1 AT4G09720 33.641 217 118 5 14 223 8 205 8.06e-31 113
MS.gene004991.t1 AT2G21880 36.970 165 97 4 14 171 9 173 8.88e-31 113
MS.gene004991.t1 AT5G64990 34.161 161 99 1 15 168 8 168 1.20e-30 112
MS.gene004991.t1 AT4G09720 32.883 222 118 5 14 223 8 210 7.72e-30 110
MS.gene004991.t1 AT5G39620 33.945 218 115 7 15 223 6 203 2.08e-29 109
MS.gene004991.t1 AT4G09720 32.456 228 117 5 14 223 8 216 4.81e-29 108
MS.gene004991.t1 AT1G49300 35.152 165 100 4 14 171 8 172 5.16e-28 105
MS.gene004991.t1 AT1G49300 35.152 165 100 4 14 171 8 172 5.16e-28 105
MS.gene004991.t1 AT5G46025 47.573 103 53 1 76 177 6 108 2.95e-25 95.9
MS.gene004991.t1 AT2G21880 35.135 148 89 4 31 171 1 148 1.12e-22 91.3
MS.gene004991.t1 AT2G21880 35.135 148 89 4 31 171 1 148 1.12e-22 91.3
MS.gene004991.t1 AT4G09720 33.690 187 98 5 44 223 4 171 1.38e-22 90.5
MS.gene004991.t1 AT4G09720 33.690 187 98 5 44 223 4 171 1.38e-22 90.5
MS.gene004991.t1 AT4G09720 33.690 187 98 5 44 223 4 171 1.38e-22 90.5
MS.gene004991.t1 AT5G55080 28.571 175 121 3 1 175 1 171 5.80e-22 90.5
MS.gene004991.t1 AT2G21880 32.121 165 97 5 14 171 9 165 5.25e-21 87.4
MS.gene004991.t1 AT2G44690 32.222 180 108 5 14 181 18 195 1.09e-20 86.7
MS.gene004991.t1 AT5G20020 31.053 190 117 7 1 189 1 177 1.18e-20 86.7
MS.gene004991.t1 AT5G20010 31.053 190 117 7 1 189 1 177 1.41e-20 86.7
MS.gene004991.t1 AT5G55190 30.114 176 117 5 1 175 1 171 1.82e-20 86.3
MS.gene004991.t1 AT4G35020 31.034 174 106 6 14 175 6 177 1.87e-17 77.8
MS.gene004991.t1 AT4G35020 31.034 174 106 6 14 175 6 177 1.87e-17 77.8
MS.gene004991.t1 AT4G35020 31.034 174 106 6 14 175 6 177 1.87e-17 77.8
MS.gene004991.t1 AT5G45970 30.233 172 106 5 14 173 6 175 2.59e-17 77.4
MS.gene004991.t1 AT4G28950 29.050 179 115 5 14 182 6 182 4.20e-17 77.0
MS.gene004991.t1 AT1G20090 30.460 174 107 6 14 175 5 176 5.34e-17 76.6
MS.gene004991.t1 AT5G62880 29.213 178 112 5 14 179 8 183 5.57e-17 77.0
MS.gene004991.t1 AT1G75840 29.885 174 108 6 14 175 6 177 6.70e-17 76.3
MS.gene004991.t1 AT3G48040 29.310 174 109 5 14 175 8 179 1.20e-16 75.9
MS.gene004991.t1 AT3G51300 30.233 172 106 6 16 175 8 177 1.77e-16 75.1
MS.gene004991.t1 AT4G35950 29.310 174 109 6 14 175 6 177 3.60e-16 74.3
MS.gene004991.t1 AT2G17800 29.310 174 109 6 14 175 6 177 5.01e-16 73.9
MS.gene004991.t1 AT2G17800 29.310 174 109 6 14 175 6 177 5.01e-16 73.9
MS.gene004991.t1 AT3G48040 29.299 157 97 5 14 158 8 162 3.74e-14 68.6
MS.gene004991.t1 AT5G62880 28.662 157 98 5 14 158 8 162 2.61e-13 66.6
MS.gene004991.t1 AT4G35020 32.456 114 74 3 16 128 8 119 5.75e-12 62.0
MS.gene004991.t1 AT4G35020 32.456 114 74 3 16 128 8 119 5.75e-12 62.0
MS.gene004991.t1 AT3G22950 31.405 121 77 3 15 134 18 133 4.09e-11 60.5
MS.gene004991.t1 AT3G22950 31.405 121 77 3 15 134 18 133 4.09e-11 60.5

Find 44 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGCACAAGGTTTGTTCTTTA+TGG 0.121880 1.4:-64053735 MS.gene004991:CDS
CCTATAGTTGACTTTGAATT+AGG 0.178199 1.4:+64054872 None:intergenic
CTGTTACTTCTGCATATTAT+AGG 0.243592 1.4:-64054760 MS.gene004991:CDS
TGTTACTTCTGCATATTATA+GGG 0.293105 1.4:-64054759 MS.gene004991:CDS
AAAGGGTTGTTGTTCGAAAT+AGG 0.304511 1.4:-64053522 None:intergenic
GGGTGCAGTTGGAGCACTTC+TGG 0.313947 1.4:-64054738 MS.gene004991:CDS
GATGCTTCCTGGATTGAAAA+TGG 0.324916 1.4:-64053599 MS.gene004991:CDS
AGGAAGTTAAAGCGCAGATA+TGG 0.333773 1.4:-64054811 MS.gene004991:CDS
TGGGAACAAGTCAGATCTTA+AGG 0.381456 1.4:-64053801 MS.gene004991:CDS
TCAAGAAAGATGATGCTTCC+TGG 0.401349 1.4:-64053610 MS.gene004991:CDS
CCTAATTCAAAGTCAACTAT+AGG 0.411802 1.4:-64054872 MS.gene004991:CDS
CTTGAGACATCATAACCTTC+CGG 0.418307 1.4:+64053636 None:intergenic
AACGTTGTCACAATACTTGT+TGG 0.431302 1.4:-64053821 MS.gene004991:CDS
CGCAGATATGGGACACAGCT+GGG 0.471236 1.4:-64054799 MS.gene004991:CDS
GCGCAGATATGGGACACAGC+TGG 0.472726 1.4:-64054800 MS.gene004991:CDS
GGGACACAGCTGGGCAAGAA+AGG 0.479969 1.4:-64054790 MS.gene004991:CDS
GGAAGTTAAAGCGCAGATAT+GGG 0.487077 1.4:-64054810 MS.gene004991:CDS
ACGTTGTCACAATACTTGTT+GGG 0.487177 1.4:-64053820 MS.gene004991:CDS
ATTTACTCGCAAGATTTGCC+AGG 0.491537 1.4:-64054907 MS.gene004991:CDS
TAATTGGTGATTCAGCTGTT+GGG 0.495291 1.4:-64054937 MS.gene004991:CDS
GCATATTATAGGGGTGCAGT+TGG 0.498462 1.4:-64054749 MS.gene004991:CDS
TTCTGGTATATGACATCAGC+AGG 0.502132 1.4:-64054721 MS.gene004991:CDS
TTAATTGGTGATTCAGCTGT+TGG 0.515554 1.4:-64054938 MS.gene004991:CDS
CGTCAAACGTTTGATAGCAT+TGG 0.518334 1.4:-64054698 MS.gene004991:CDS
GAAGGGGATCGATCAGCAGA+AGG 0.552374 1.4:-64053554 MS.gene004991:CDS
GTTACTTCTGCATATTATAG+GGG 0.554984 1.4:-64054758 MS.gene004991:CDS
ACTGTTGTTCTACAACAAGA+AGG 0.557959 1.4:-64053572 MS.gene004991:CDS
ATTAGGATAAAATTCATCCC+TGG 0.561389 1.4:+64054889 None:intergenic
CGTTTGATAGCATTGGTCGA+TGG 0.567811 1.4:-64054691 MS.gene004991:CDS
CTGTTGTTCTACAACAAGAA+GGG 0.571816 1.4:-64053571 MS.gene004991:CDS
AGATCTTAAGGATGCGAGAG+AGG 0.573972 1.4:-64053789 MS.gene004991:CDS
AAATCTACAACATATTAAGC+CGG 0.576659 1.4:-64053655 MS.gene004991:CDS
AGGGGATCGATCAGCAGAAG+GGG 0.577182 1.4:-64053552 MS.gene004991:CDS
AAGGGGATCGATCAGCAGAA+GGG 0.592624 1.4:-64053553 MS.gene004991:CDS
GGTACCTACGACGGAAGGCA+AGG 0.605827 1.4:-64053768 MS.gene004991:CDS
AGAGAGGTACCTACGACGGA+AGG 0.610104 1.4:-64053773 MS.gene004991:CDS
TAATGAAGAAGAGAAGACAG+AGG 0.611987 1.4:-64054981 MS.gene004991:CDS
TGTTGTTCTACAACAAGAAG+GGG 0.621100 1.4:-64053570 MS.gene004991:CDS
AACGCCTTGCCTTCCGTCGT+AGG 0.621241 1.4:+64053764 None:intergenic
CTACAACATATTAAGCCGGA+AGG 0.622382 1.4:-64053651 MS.gene004991:CDS
TTTACTCGCAAGATTTGCCA+GGG 0.623585 1.4:-64054906 MS.gene004991:CDS
AAGGCGTTAGCTGAGGCACA+AGG 0.638041 1.4:-64053749 MS.gene004991:CDS
GGAAGGCAAGGCGTTAGCTG+AGG 0.641771 1.4:-64053756 MS.gene004991:CDS
TGCGAGAGAGGTACCTACGA+CGG 0.743487 1.4:-64053777 MS.gene004991:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTATTTGTATATACTTTA+GGG - chr1.4:64054371-64054390 MS.gene004991:intron 10.0%
!! TAATTATTTGTATATACTTT+AGG - chr1.4:64054370-64054389 MS.gene004991:intron 10.0%
!!! AATTTTGTTTATTTACATAT+AGG + chr1.4:64054064-64054083 None:intergenic 10.0%
!! CAAAAAAAAAAAAACGTATA+TGG + chr1.4:64054320-64054339 None:intergenic 15.0%
!! TTTATGATACTGAAAATTAA+TGG - chr1.4:64053994-64054013 MS.gene004991:intron 15.0%
!!! GTTTTTTTTTTTTTGAAGAA+GGG - chr1.4:64054325-64054344 MS.gene004991:intron 15.0%
!!! TTTTTTTTTTTTTGAAGAAG+GGG - chr1.4:64054326-64054345 MS.gene004991:intron 15.0%
!! AAAGAACAAGAAAAATTTCA+AGG + chr1.4:64053900-64053919 None:intergenic 20.0%
!! ACTCAAAAAATGGAAATTAA+CGG - chr1.4:64053679-64053698 MS.gene004991:CDS 20.0%
!! AGTCTAAGTATATGAAAATA+TGG + chr1.4:64054284-64054303 None:intergenic 20.0%
!! TCATATACTTAGACTAAAAT+GGG - chr1.4:64054289-64054308 MS.gene004991:intron 20.0%
!! TTCATATACTTAGACTAAAA+TGG - chr1.4:64054288-64054307 MS.gene004991:intron 20.0%
!!! AAAAACTTTAGAAACTAAGT+TGG + chr1.4:64054192-64054211 None:intergenic 20.0%
!!! AAACTTTAGAGATTAAATTG+AGG + chr1.4:64054161-64054180 None:intergenic 20.0%
!!! AACTTTAGAGATTAAATTGA+GGG + chr1.4:64054160-64054179 None:intergenic 20.0%
!!! CAATTAAGACAATTTTGAAA+AGG + chr1.4:64053564-64053583 None:intergenic 20.0%
!!! CGTTTTTTTTTTTTTGAAGA+AGG - chr1.4:64054324-64054343 MS.gene004991:intron 20.0%
!!! CTTTTCAAAATTGTCTTAAT+TGG - chr1.4:64053562-64053581 MS.gene004991:CDS 20.0%
!!! TAAGCAAATTTTGAAGTATA+TGG - chr1.4:64054220-64054239 MS.gene004991:intron 20.0%
!!! TAATTTCCATTTTTTGAGTT+TGG + chr1.4:64053678-64053697 None:intergenic 20.0%
! AAAAATGGAAATTAACGGAA+AGG - chr1.4:64053684-64053703 MS.gene004991:CDS 25.0%
! AAATCTACAACATATTAAGC+CGG - chr1.4:64054860-64054879 MS.gene004991:CDS 25.0%
! ACAAAAAAACTTTACACTGT+TGG + chr1.4:64054468-64054487 None:intergenic 25.0%
! ATATACTTAGACTAAAATGG+GGG - chr1.4:64054291-64054310 MS.gene004991:intron 25.0%
! CATATACTTAGACTAAAATG+GGG - chr1.4:64054290-64054309 MS.gene004991:intron 25.0%
! TGAATCAAAGCAAAAACAAA+AGG + chr1.4:64054582-64054601 None:intergenic 25.0%
! TTTCTCAATAAACTTGACAT+TGG - chr1.4:64053955-64053974 MS.gene004991:intron 25.0%
!! AATGGTTTACTCTTCATTTT+AGG - chr1.4:64054426-64054445 MS.gene004991:intron 25.0%
!! TGTTACTTCTGCATATTATA+GGG - chr1.4:64053756-64053775 MS.gene004991:CDS 25.0%
!!! TTGTTCTTTCTATGTTTTTG+AGG - chr1.4:64053911-64053930 MS.gene004991:intron 25.0%
AAACATGTACTCGCATATAT+TGG - chr1.4:64054603-64054622 MS.gene004991:intron 30.0%
AGAGTTCCAAACTCAAAAAA+TGG - chr1.4:64053669-64053688 MS.gene004991:CDS 30.0%
AGGACCATATAAGTACTATA+AGG + chr1.4:64054044-64054063 None:intergenic 30.0%
ATTAGGATAAAATTCATCCC+TGG + chr1.4:64053629-64053648 None:intergenic 30.0%
CATTAAACTTTCCCCAAAAA+GGG + chr1.4:64054020-64054039 None:intergenic 30.0%
CCTAATTCAAAGTCAACTAT+AGG - chr1.4:64053643-64053662 MS.gene004991:CDS 30.0%
GTTACTTCTGCATATTATAG+GGG - chr1.4:64053757-64053776 MS.gene004991:CDS 30.0%
TACAAAATAAAATCGCCTCA+AGG + chr1.4:64054105-64054124 None:intergenic 30.0%
TACATGTACACACATATTGA+TGG - chr1.4:64054547-64054566 MS.gene004991:intron 30.0%
! AAAATTAATGGCCCTTTTTG+GGG - chr1.4:64054006-64054025 MS.gene004991:intron 30.0%
! CCTATAGTTGACTTTGAATT+AGG + chr1.4:64053646-64053665 None:intergenic 30.0%
! CTGTTACTTCTGCATATTAT+AGG - chr1.4:64053755-64053774 MS.gene004991:CDS 30.0%
! GAAAATTAATGGCCCTTTTT+GGG - chr1.4:64054005-64054024 MS.gene004991:intron 30.0%
! TGAAAATTAATGGCCCTTTT+TGG - chr1.4:64054004-64054023 MS.gene004991:intron 30.0%
!! ATGGCCTTATAGTACTTATA+TGG - chr1.4:64054037-64054056 MS.gene004991:intron 30.0%
!!! TGTTTTTGAGGTAAATGATC+AGG - chr1.4:64053923-64053942 MS.gene004991:intron 30.0%
AACGTTGTCACAATACTTGT+TGG - chr1.4:64054694-64054713 MS.gene004991:CDS 35.0%
ACGTTGTCACAATACTTGTT+GGG - chr1.4:64054695-64054714 MS.gene004991:CDS 35.0%
ACTGTTGTTCTACAACAAGA+AGG - chr1.4:64054943-64054962 MS.gene004991:CDS 35.0%
AGTGATACTTCAAAGGCTAA+TGG - chr1.4:64054408-64054427 MS.gene004991:intron 35.0%
CCATTAAACTTTCCCCAAAA+AGG + chr1.4:64054021-64054040 None:intergenic 35.0%
CCTCAAGGAAAAGAATGAAA+AGG + chr1.4:64054090-64054109 None:intergenic 35.0%
CTGTTGTTCTACAACAAGAA+GGG - chr1.4:64054944-64054963 MS.gene004991:CDS 35.0%
GAAATGATGATTAAGTGCAC+TGG - chr1.4:64054642-64054661 MS.gene004991:intron 35.0%
TAATGAAGAAGAGAAGACAG+AGG - chr1.4:64053534-64053553 MS.gene004991:CDS 35.0%
TGAAGAGAGTGATACTTCAA+AGG - chr1.4:64054401-64054420 MS.gene004991:intron 35.0%
TGTTGTTCTACAACAAGAAG+GGG - chr1.4:64054945-64054964 MS.gene004991:CDS 35.0%
! AGTCTTTCCATTTTCAATCC+AGG + chr1.4:64054926-64054945 None:intergenic 35.0%
! CCTTTTCATTCTTTTCCTTG+AGG - chr1.4:64054087-64054106 MS.gene004991:intron 35.0%
! TAATTGGTGATTCAGCTGTT+GGG - chr1.4:64053578-64053597 MS.gene004991:CDS 35.0%
! TTAATTGGTGATTCAGCTGT+TGG - chr1.4:64053577-64053596 MS.gene004991:CDS 35.0%
!! CCTTTTTGGGGAAAGTTTAA+TGG - chr1.4:64054018-64054037 MS.gene004991:intron 35.0%
AGGAAGTTAAAGCGCAGATA+TGG - chr1.4:64053704-64053723 MS.gene004991:CDS 40.0%
ATTTACTCGCAAGATTTGCC+AGG - chr1.4:64053608-64053627 MS.gene004991:CDS 40.0%
CTACAACATATTAAGCCGGA+AGG - chr1.4:64054864-64054883 MS.gene004991:CDS 40.0%
CTTGAGACATCATAACCTTC+CGG + chr1.4:64054882-64054901 None:intergenic 40.0%
GATGCTTCCTGGATTGAAAA+TGG - chr1.4:64054916-64054935 MS.gene004991:CDS 40.0%
GGAAGTTAAAGCGCAGATAT+GGG - chr1.4:64053705-64053724 MS.gene004991:CDS 40.0%
TCAAGAAAGATGATGCTTCC+TGG - chr1.4:64054905-64054924 MS.gene004991:CDS 40.0%
TGGGAACAAGTCAGATCTTA+AGG - chr1.4:64054714-64054733 MS.gene004991:CDS 40.0%
TTCTGGTATATGACATCAGC+AGG - chr1.4:64053794-64053813 MS.gene004991:CDS 40.0%
TTTACTCGCAAGATTTGCCA+GGG - chr1.4:64053609-64053628 MS.gene004991:CDS 40.0%
! CGTCAAACGTTTGATAGCAT+TGG - chr1.4:64053817-64053836 MS.gene004991:CDS 40.0%
! GGCACAAGGTTTGTTCTTTA+TGG - chr1.4:64054780-64054799 MS.gene004991:CDS 40.0%
AGATCTTAAGGATGCGAGAG+AGG - chr1.4:64054726-64054745 MS.gene004991:CDS 45.0%
AGCAGAAGGGGAGACAAAAA+AGG - chr1.4:64054975-64054994 MS.gene004991:CDS 45.0%
GCAGAAGGGGAGACAAAAAA+GGG - chr1.4:64054976-64054995 MS.gene004991:CDS 45.0%
! GCATATTATAGGGGTGCAGT+TGG - chr1.4:64053766-64053785 MS.gene004991:CDS 45.0%
!! CGTTTGATAGCATTGGTCGA+TGG - chr1.4:64053824-64053843 MS.gene004991:CDS 45.0%
AAGGGGATCGATCAGCAGAA+GGG - chr1.4:64054962-64054981 MS.gene004991:CDS 50.0%
AGGGGATCGATCAGCAGAAG+GGG - chr1.4:64054963-64054982 MS.gene004991:CDS 55.0%
CGCAGATATGGGACACAGCT+GGG - chr1.4:64053716-64053735 MS.gene004991:CDS 55.0%
GAAGGGGATCGATCAGCAGA+AGG - chr1.4:64054961-64054980 MS.gene004991:CDS 55.0%
! AAGGCGTTAGCTGAGGCACA+AGG - chr1.4:64054766-64054785 MS.gene004991:CDS 55.0%
!! AGAGAGGTACCTACGACGGA+AGG - chr1.4:64054742-64054761 MS.gene004991:CDS 55.0%
!! TGCGAGAGAGGTACCTACGA+CGG - chr1.4:64054738-64054757 MS.gene004991:CDS 55.0%
AACGCCTTGCCTTCCGTCGT+AGG + chr1.4:64054754-64054773 None:intergenic 60.0%
GCGCAGATATGGGACACAGC+TGG - chr1.4:64053715-64053734 MS.gene004991:CDS 60.0%
GGGACACAGCTGGGCAAGAA+AGG - chr1.4:64053725-64053744 MS.gene004991:CDS 60.0%
GGTACCTACGACGGAAGGCA+AGG - chr1.4:64054747-64054766 MS.gene004991:CDS 60.0%
! GGAAGGCAAGGCGTTAGCTG+AGG - chr1.4:64054759-64054778 MS.gene004991:CDS 60.0%
! GGGTGCAGTTGGAGCACTTC+TGG - chr1.4:64053777-64053796 MS.gene004991:CDS 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 64053523 64055014 64053523 ID=MS.gene004991
chr1.4 mRNA 64053523 64055014 64053523 ID=MS.gene004991.t1;Parent=MS.gene004991
chr1.4 exon 64054675 64055014 64054675 ID=MS.gene004991.t1.exon1;Parent=MS.gene004991.t1
chr1.4 CDS 64054675 64055014 64054675 ID=cds.MS.gene004991.t1;Parent=MS.gene004991.t1
chr1.4 exon 64053523 64053857 64053523 ID=MS.gene004991.t1.exon2;Parent=MS.gene004991.t1
chr1.4 CDS 64053523 64053857 64053523 ID=cds.MS.gene004991.t1;Parent=MS.gene004991.t1
Gene Sequence

>MS.gene004991

ATGGCATTTAATAATGAAGAAGAGAAGACAGAGGATTACCTTTTCAAAATTGTCTTAATTGGTGATTCAGCTGTTGGGAAATCAAATTTACTCGCAAGATTTGCCAGGGATGAATTTTATCCTAATTCAAAGTCAACTATAGGAGTAGAGTTCCAAACTCAAAAAATGGAAATTAACGGAAAGGAAGTTAAAGCGCAGATATGGGACACAGCTGGGCAAGAAAGGTTCAGAGCTGTTACTTCTGCATATTATAGGGGTGCAGTTGGAGCACTTCTGGTATATGACATCAGCAGGCGTCAAACGTTTGATAGCATTGGTCGATGGCTTAACGAACTTCACAGTAAGAATTATAACTATGTTTGAATCTTTCTTTGCCTTGAAATTTTTCTTGTTCTTTCTATGTTTTTGAGGTAAATGATCAGGAACTGTTAATTTCTCAATAAACTTGACATTGGTACAATATAGAAAACATTTATGATACTGAAAATTAATGGCCCTTTTTGGGGAAAGTTTAATGGCCTTATAGTACTTATATGGTCCTATATGTAAATAAACAAAATTATGCCTTTTCATTCTTTTCCTTGAGGCGATTTTATTTTGTAAATACTAAATTGCAAATTTTCATTTGAATAAACCCTCAATTTAATCTCTAAAGTTTCAGTCTCTCCAACTTAGTTTCTAAAGTTTTTAAAAAGAGTAAGCAAATTTTGAAGTATATGGAACTCGTCACTTTAGTATATGCTTTTAAGACTAACACGCCATATTTTCATATACTTAGACTAAAATGGGGGATGCCATATACGTTTTTTTTTTTTTGAAGAAGGGGATGTCATATACGTTAAGAGACTAATTATTTGTATATACTTTAGGGACTAAATTGAAGAGAGTGATACTTCAAAGGCTAATGGTTTACTCTTCATTTTAGGTTCTTAAGCTACTTCACCAACAGTGTAAAGTTTTTTTGTGTCTTCTCATTTGAATTTGAACTTCTGCATCATTATGCTAGTCTTGTTATTCTTCGACATACATGTACACACATATTGATGGTTGTTTTTTCCTTTTGTTTTTGCTTTGATTCAAAAACATGTACTCGCATATATTGGTAGTTGTGAATATGATGAAATGATGATTAAGTGCACTGGTGTATTATTTAACAGCTCACTCTGATATGAACGTTGTCACAATACTTGTTGGGAACAAGTCAGATCTTAAGGATGCGAGAGAGGTACCTACGACGGAAGGCAAGGCGTTAGCTGAGGCACAAGGTTTGTTCTTTATGGAGACATCAGCACTTGATTCATCAAATGTAGTTGCTGCTTTTCAAACAGTTGTAAAAGAAATCTACAACATATTAAGCCGGAAGGTTATGATGTCTCAAGAGCTCAAGAAAGATGATGCTTCCTGGATTGAAAATGGAAAGACTGTTGTTCTACAACAAGAAGGGGATCGATCAGCAGAAGGGGAGACAAAAAAGGGTTGTTGTTCGAAATAG
Protein sequence

>MS.gene004991.t1

MAFNNEEEKTEDYLFKIVLIGDSAVGKSNLLARFARDEFYPNSKSTIGVEFQTQKMEINGKEVKAQIWDTAGQERFRAVTSAYYRGAVGALLVYDISRRQTFDSIGRWLNELHTHSDMNVVTILVGNKSDLKDAREVPTTEGKALAEAQGLFFMETSALDSSNVVAAFQTVVKEIYNILSRKVMMSQELKKDDASWIENGKTVVLQQEGDRSAEGETKKGCCSK