Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54573.t1 | XP_013456272.1 | 91.1 | 584 | 6 | 1 | 1 | 538 | 1 | 584 | 6.00E-306 | 1059.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54573.t1 | Q84RR0 | 71.8 | 529 | 101 | 3 | 1 | 482 | 1 | 528 | 6.6e-229 | 794.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54573.t1 | A0A072UML4 | 91.1 | 584 | 6 | 1 | 1 | 538 | 1 | 584 | 4.3e-306 | 1059.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04918 | MS.gene54573 | 0.816512 | 5.32E-52 | -1.69E-46 |
| MS.gene04919 | MS.gene54573 | 0.827439 | 1.56E-54 | -1.69E-46 |
| MS.gene04920 | MS.gene54573 | 0.844109 | 9.27E-59 | -1.69E-46 |
| MS.gene049283 | MS.gene54573 | 0.871242 | 7.93E-67 | -1.69E-46 |
| MS.gene049411 | MS.gene54573 | 0.820116 | 8.13E-53 | -1.69E-46 |
| MS.gene049499 | MS.gene54573 | 0.84648 | 2.12E-59 | -1.69E-46 |
| MS.gene049680 | MS.gene54573 | 0.815161 | 1.07E-51 | -1.69E-46 |
| MS.gene049983 | MS.gene54573 | 0.814697 | 1.35E-51 | -1.69E-46 |
| MS.gene050084 | MS.gene54573 | 0.805172 | 1.52E-49 | -1.69E-46 |
| MS.gene050085 | MS.gene54573 | 0.830336 | 3.11E-55 | -1.69E-46 |
| MS.gene050271 | MS.gene54573 | 0.811877 | 5.62E-51 | -1.69E-46 |
| MS.gene050359 | MS.gene54573 | 0.804828 | 1.79E-49 | -1.69E-46 |
| MS.gene050384 | MS.gene54573 | 0.827621 | 1.41E-54 | -1.69E-46 |
| MS.gene050386 | MS.gene54573 | 0.831289 | 1.82E-55 | -1.69E-46 |
| MS.gene050414 | MS.gene54573 | 0.842534 | 2.44E-58 | -1.69E-46 |
| MS.gene050646 | MS.gene54573 | 0.872441 | 3.17E-67 | -1.69E-46 |
| MS.gene050654 | MS.gene54573 | 0.818859 | 1.57E-52 | -1.69E-46 |
| MS.gene050931 | MS.gene54573 | 0.801521 | 8.66E-49 | -1.69E-46 |
| MS.gene050945 | MS.gene54573 | 0.901062 | 3.92E-78 | -1.69E-46 |
| MS.gene051003 | MS.gene54573 | 0.811826 | 5.77E-51 | -1.69E-46 |
| MS.gene051043 | MS.gene54573 | 0.858013 | 1.10E-62 | -1.69E-46 |
| MS.gene051234 | MS.gene54573 | 0.826752 | 2.28E-54 | -1.69E-46 |
| MS.gene051343 | MS.gene54573 | 0.846282 | 2.40E-59 | -1.69E-46 |
| MS.gene051363 | MS.gene54573 | 0.802659 | 5.05E-49 | -1.69E-46 |
| MS.gene051365 | MS.gene54573 | 0.808279 | 3.35E-50 | -1.69E-46 |
| MS.gene051597 | MS.gene54573 | 0.833991 | 3.88E-56 | -1.69E-46 |
| MS.gene051676 | MS.gene54573 | 0.80542 | 1.35E-49 | -1.69E-46 |
| MS.gene051699 | MS.gene54573 | 0.827109 | 1.88E-54 | -1.69E-46 |
| MS.gene051731 | MS.gene54573 | 0.819308 | 1.24E-52 | -1.69E-46 |
| MS.gene051856 | MS.gene54573 | 0.815739 | 7.92E-52 | -1.69E-46 |
| MS.gene051902 | MS.gene54573 | 0.850534 | 1.60E-60 | -1.69E-46 |
| MS.gene051975 | MS.gene54573 | 0.814341 | 1.62E-51 | -1.69E-46 |
| MS.gene052072 | MS.gene54573 | 0.890318 | 1.10E-73 | -1.69E-46 |
| MS.gene052242 | MS.gene54573 | -0.824166 | 9.35E-54 | -1.69E-46 |
| MS.gene052296 | MS.gene54573 | 0.80546 | 1.32E-49 | -1.69E-46 |
| MS.gene052307 | MS.gene54573 | 0.822283 | 2.57E-53 | -1.69E-46 |
| MS.gene052342 | MS.gene54573 | 0.806015 | 1.01E-49 | -1.69E-46 |
| MS.gene052533 | MS.gene54573 | 0.819646 | 1.04E-52 | -1.69E-46 |
| MS.gene052609 | MS.gene54573 | 0.801088 | 1.06E-48 | -1.69E-46 |
| MS.gene052793 | MS.gene54573 | 0.832772 | 7.81E-56 | -1.69E-46 |
| MS.gene053058 | MS.gene54573 | 0.820407 | 6.97E-53 | -1.69E-46 |
| MS.gene053542 | MS.gene54573 | 0.807575 | 4.73E-50 | -1.69E-46 |
| MS.gene053564 | MS.gene54573 | 0.811629 | 6.36E-51 | -1.69E-46 |
| MS.gene053575 | MS.gene54573 | 0.800134 | 1.66E-48 | -1.69E-46 |
| MS.gene053879 | MS.gene54573 | 0.819529 | 1.11E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene54573 | 0.836981 | 6.80E-57 | -1.69E-46 |
| MS.gene053963 | MS.gene54573 | 0.810274 | 1.25E-50 | -1.69E-46 |
| MS.gene054004 | MS.gene54573 | 0.834289 | 3.27E-56 | -1.69E-46 |
| MS.gene054222 | MS.gene54573 | 0.801806 | 7.57E-49 | -1.69E-46 |
| MS.gene054403 | MS.gene54573 | 0.804661 | 1.94E-49 | -1.69E-46 |
| MS.gene054413 | MS.gene54573 | 0.876654 | 1.18E-68 | -1.69E-46 |
| MS.gene054709 | MS.gene54573 | 0.83275 | 7.91E-56 | -1.69E-46 |
| MS.gene054957 | MS.gene54573 | 0.835912 | 1.27E-56 | -1.69E-46 |
| MS.gene055150 | MS.gene54573 | 0.80635 | 8.59E-50 | -1.69E-46 |
| MS.gene055841 | MS.gene54573 | 0.807951 | 3.93E-50 | -1.69E-46 |
| MS.gene056045 | MS.gene54573 | 0.815335 | 9.75E-52 | -1.69E-46 |
| MS.gene056097 | MS.gene54573 | 0.816889 | 4.38E-52 | -1.69E-46 |
| MS.gene056181 | MS.gene54573 | 0.810638 | 1.04E-50 | -1.69E-46 |
| MS.gene056578 | MS.gene54573 | 0.843054 | 1.77E-58 | -1.69E-46 |
| MS.gene056786 | MS.gene54573 | 0.803265 | 3.79E-49 | -1.69E-46 |
| MS.gene05686 | MS.gene54573 | 0.806597 | 7.62E-50 | -1.69E-46 |
| MS.gene056973 | MS.gene54573 | 0.805226 | 1.48E-49 | -1.69E-46 |
| MS.gene056986 | MS.gene54573 | 0.815146 | 1.07E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene54573 | 0.8369 | 7.13E-57 | -1.69E-46 |
| MS.gene057174 | MS.gene54573 | 0.81311 | 3.02E-51 | -1.69E-46 |
| MS.gene057208 | MS.gene54573 | 0.807279 | 5.46E-50 | -1.69E-46 |
| MS.gene057622 | MS.gene54573 | 0.823251 | 1.53E-53 | -1.69E-46 |
| MS.gene05781 | MS.gene54573 | 0.833611 | 4.83E-56 | -1.69E-46 |
| MS.gene057890 | MS.gene54573 | -0.804064 | 2.58E-49 | -1.69E-46 |
| MS.gene057999 | MS.gene54573 | 0.819159 | 1.34E-52 | -1.69E-46 |
| MS.gene058008 | MS.gene54573 | 0.813972 | 1.95E-51 | -1.69E-46 |
| MS.gene058054 | MS.gene54573 | 0.818186 | 2.24E-52 | -1.69E-46 |
| MS.gene058074 | MS.gene54573 | 0.838944 | 2.13E-57 | -1.69E-46 |
| MS.gene058159 | MS.gene54573 | 0.81353 | 2.44E-51 | -1.69E-46 |
| MS.gene058292 | MS.gene54573 | 0.804044 | 2.61E-49 | -1.69E-46 |
| MS.gene058781 | MS.gene54573 | 0.833871 | 4.16E-56 | -1.69E-46 |
| MS.gene059007 | MS.gene54573 | 0.813384 | 2.63E-51 | -1.69E-46 |
| MS.gene059146 | MS.gene54573 | 0.86701 | 1.87E-65 | -1.69E-46 |
| MS.gene05952 | MS.gene54573 | 0.826067 | 3.32E-54 | -1.69E-46 |
| MS.gene059590 | MS.gene54573 | 0.805472 | 1.31E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene54573 | -0.847814 | 9.12E-60 | -1.69E-46 |
| MS.gene06018 | MS.gene54573 | 0.816914 | 4.33E-52 | -1.69E-46 |
| MS.gene060244 | MS.gene54573 | 0.825774 | 3.90E-54 | -1.69E-46 |
| MS.gene060527 | MS.gene54573 | 0.801163 | 1.02E-48 | -1.69E-46 |
| MS.gene060763 | MS.gene54573 | 0.814866 | 1.24E-51 | -1.69E-46 |
| MS.gene060933 | MS.gene54573 | 0.883286 | 5.10E-71 | -1.69E-46 |
| MS.gene060935 | MS.gene54573 | 0.880066 | 7.45E-70 | -1.69E-46 |
| MS.gene060937 | MS.gene54573 | 0.893911 | 4.04E-75 | -1.69E-46 |
| MS.gene061100 | MS.gene54573 | 0.829638 | 4.60E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54573.t1 | MTR_4g066650 | 91.096 | 584 | 6 | 1 | 1 | 538 | 1 | 584 | 0.0 | 1101 |
| MS.gene54573.t1 | MTR_2g098580 | 84.884 | 516 | 76 | 1 | 25 | 538 | 81 | 596 | 0.0 | 923 |
| MS.gene54573.t1 | MTR_3g065380 | 68.213 | 582 | 131 | 5 | 1 | 536 | 1 | 574 | 0.0 | 798 |
| MS.gene54573.t1 | MTR_3g065410 | 72.852 | 512 | 116 | 3 | 25 | 536 | 64 | 552 | 0.0 | 766 |
| MS.gene54573.t1 | MTR_5g097670 | 84.566 | 311 | 48 | 0 | 177 | 487 | 4 | 314 | 0.0 | 558 |
| MS.gene54573.t1 | MTR_4g075010 | 40.173 | 463 | 208 | 9 | 26 | 473 | 17 | 425 | 1.29e-111 | 344 |
| MS.gene54573.t1 | MTR_4g075030 | 38.933 | 375 | 176 | 13 | 1 | 349 | 7 | 354 | 2.02e-73 | 238 |
| MS.gene54573.t1 | MTR_4g068940 | 29.461 | 482 | 301 | 11 | 24 | 483 | 56 | 520 | 6.53e-60 | 208 |
| MS.gene54573.t1 | MTR_4g068940 | 29.461 | 482 | 301 | 11 | 24 | 483 | 56 | 520 | 7.92e-60 | 208 |
| MS.gene54573.t1 | MTR_4g096720 | 53.030 | 66 | 28 | 1 | 197 | 259 | 58 | 123 | 3.34e-16 | 76.6 |
| MS.gene54573.t1 | MTR_3g462590 | 24.359 | 234 | 166 | 7 | 56 | 283 | 62 | 290 | 3.13e-14 | 73.9 |
| MS.gene54573.t1 | MTR_3g462570 | 23.552 | 259 | 171 | 10 | 78 | 322 | 33 | 278 | 4.38e-12 | 68.9 |
| MS.gene54573.t1 | MTR_1g105460 | 26.351 | 296 | 183 | 12 | 18 | 287 | 16 | 302 | 8.51e-12 | 66.6 |
| MS.gene54573.t1 | MTR_8g022900 | 25.837 | 209 | 136 | 8 | 91 | 286 | 63 | 265 | 1.09e-11 | 65.9 |
| MS.gene54573.t1 | MTR_2g044440 | 25.263 | 190 | 134 | 5 | 83 | 267 | 88 | 274 | 1.66e-11 | 65.5 |
| MS.gene54573.t1 | MTR_5g078410 | 23.556 | 225 | 143 | 5 | 67 | 283 | 127 | 330 | 2.04e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54573.t1 | AT2G31510 | 70.515 | 563 | 114 | 5 | 1 | 513 | 1 | 561 | 0.0 | 804 |
| MS.gene54573.t1 | AT1G65430 | 72.320 | 513 | 134 | 1 | 26 | 538 | 63 | 567 | 0.0 | 797 |
| MS.gene54573.t1 | AT2G31510 | 76.892 | 489 | 109 | 2 | 28 | 513 | 2 | 489 | 0.0 | 791 |
| MS.gene54573.t1 | AT2G31510 | 71.543 | 499 | 94 | 3 | 1 | 452 | 1 | 498 | 0.0 | 748 |
| MS.gene54573.t1 | AT1G05890 | 74.459 | 462 | 115 | 2 | 26 | 487 | 67 | 525 | 0.0 | 739 |
| MS.gene54573.t1 | AT1G05890 | 74.459 | 462 | 115 | 2 | 26 | 487 | 109 | 567 | 0.0 | 739 |
| MS.gene54573.t1 | AT2G31770 | 58.811 | 471 | 168 | 7 | 16 | 476 | 46 | 500 | 0.0 | 580 |
| MS.gene54573.t1 | AT2G31760 | 60.000 | 450 | 161 | 6 | 26 | 473 | 55 | 487 | 0.0 | 571 |
| MS.gene54573.t1 | AT2G31780 | 56.829 | 410 | 155 | 6 | 5 | 405 | 45 | 441 | 4.35e-172 | 494 |
| MS.gene54573.t1 | AT1G05880 | 38.854 | 471 | 254 | 9 | 16 | 477 | 42 | 487 | 1.39e-104 | 323 |
| MS.gene54573.t1 | AT1G05880 | 38.641 | 471 | 248 | 11 | 16 | 477 | 42 | 480 | 3.17e-100 | 311 |
| MS.gene54573.t1 | AT1G05880 | 37.969 | 453 | 247 | 9 | 16 | 459 | 42 | 469 | 2.20e-94 | 296 |
| MS.gene54573.t1 | AT4G34370 | 31.343 | 469 | 276 | 15 | 26 | 470 | 53 | 499 | 6.80e-65 | 222 |
| MS.gene54573.t1 | AT2G16090 | 29.868 | 529 | 309 | 15 | 26 | 513 | 53 | 560 | 1.27e-60 | 210 |
| MS.gene54573.t1 | AT2G16090 | 29.254 | 523 | 314 | 13 | 26 | 513 | 53 | 554 | 1.16e-59 | 207 |
| MS.gene54573.t1 | AT2G16090 | 30.490 | 469 | 281 | 12 | 26 | 470 | 53 | 500 | 2.21e-59 | 206 |
| MS.gene54573.t1 | AT2G16090 | 29.865 | 519 | 302 | 15 | 36 | 513 | 3 | 500 | 5.64e-59 | 204 |
| MS.gene54573.t1 | AT3G27710 | 29.828 | 466 | 285 | 14 | 26 | 470 | 55 | 499 | 2.49e-58 | 203 |
| MS.gene54573.t1 | AT2G16090 | 29.779 | 497 | 289 | 14 | 36 | 493 | 3 | 478 | 1.53e-57 | 199 |
| MS.gene54573.t1 | AT5G63750 | 30.290 | 482 | 280 | 15 | 30 | 473 | 25 | 488 | 1.95e-57 | 201 |
| MS.gene54573.t1 | AT5G08730 | 28.070 | 456 | 292 | 10 | 29 | 477 | 23 | 449 | 9.29e-54 | 190 |
| MS.gene54573.t1 | AT4G34370 | 33.524 | 349 | 192 | 12 | 141 | 470 | 5 | 332 | 6.73e-48 | 172 |
| MS.gene54573.t1 | AT5G63730 | 27.119 | 531 | 306 | 17 | 23 | 534 | 18 | 486 | 8.13e-48 | 174 |
| MS.gene54573.t1 | AT3G27720 | 32.047 | 337 | 190 | 10 | 153 | 470 | 143 | 459 | 5.26e-43 | 160 |
| MS.gene54573.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 67 | 534 | 2 | 439 | 5.79e-43 | 159 |
| MS.gene54573.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 67 | 534 | 2 | 439 | 5.79e-43 | 159 |
| MS.gene54573.t1 | AT5G63760 | 27.711 | 498 | 270 | 21 | 67 | 534 | 2 | 439 | 5.79e-43 | 159 |
| MS.gene54573.t1 | AT5G63760 | 28.021 | 389 | 207 | 15 | 162 | 534 | 2 | 333 | 1.08e-33 | 131 |
| MS.gene54573.t1 | AT5G10370 | 27.490 | 251 | 168 | 10 | 41 | 284 | 1512 | 1755 | 5.94e-12 | 69.3 |
| MS.gene54573.t1 | AT2G26135 | 26.291 | 213 | 139 | 8 | 75 | 279 | 140 | 342 | 1.25e-11 | 67.0 |
| MS.gene54573.t1 | AT3G45580 | 29.469 | 207 | 124 | 10 | 90 | 283 | 172 | 369 | 2.64e-11 | 66.2 |
Find 121 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCAGCAAAATTCAAATTTAC+AGG | 0.232442 | 4.3:+37354717 | None:intergenic |
| ATCGACGTTGATTGCAAATC+TGG | 0.244897 | 4.3:+37351112 | None:intergenic |
| TTCGATTGGTCGCTTGCATT+TGG | 0.271459 | 4.3:+37354777 | None:intergenic |
| TATCTCACCTCGCTAGTATT+AGG | 0.330743 | 4.3:+37356479 | None:intergenic |
| TGGCCCACCGCTCATAATAA+TGG | 0.335297 | 4.3:+37354109 | None:intergenic |
| CCCACTTGTGGCGCTGCTAT+TGG | 0.344918 | 4.3:-37355941 | MS.gene54573:CDS |
| GGGAGCAGTAAGGGGAGAGC+TGG | 0.349500 | 4.3:-37351234 | MS.gene54573:CDS |
| AGTCATTGAATTCTTTAGAT+TGG | 0.359218 | 4.3:+37351487 | None:intergenic |
| TCGATTGGTCGCTTGCATTT+GGG | 0.365336 | 4.3:+37354778 | None:intergenic |
| GTCACAGCTTAAGTTTATTA+CGG | 0.378239 | 4.3:-37351985 | MS.gene54573:CDS |
| CCGAAAAGCGGTTGGCTTGT+TGG | 0.378590 | 4.3:-37356522 | MS.gene54573:CDS |
| CTATCTGATGTAGATGCTAA+TGG | 0.382104 | 4.3:-37351297 | MS.gene54573:CDS |
| TATGCACGATGCTCACGATA+TGG | 0.384878 | 4.3:-37358092 | MS.gene54573:CDS |
| TACTACTTCGTCACCACAAT+TGG | 0.386991 | 4.3:-37357108 | MS.gene54573:intron |
| GGGAGAGCTGGGAGAGGAAA+GGG | 0.387635 | 4.3:-37351222 | MS.gene54573:CDS |
| TTATATCGAAGACAATAAAA+AGG | 0.396653 | 4.3:-37355849 | MS.gene54573:intron |
| ATCCATACTGTTTCTCATGC+TGG | 0.403926 | 4.3:-37356284 | MS.gene54573:CDS |
| GTATTAGGATGCTGGACAAC+TGG | 0.406004 | 4.3:+37356494 | None:intergenic |
| AACTATCCTCGCTCTGGGAT+TGG | 0.407143 | 4.3:-37356324 | MS.gene54573:CDS |
| TATCTGATGTAGATGCTAAT+GGG | 0.411020 | 4.3:-37351296 | MS.gene54573:CDS |
| GACTTTCGTACAAAATTAGC+TGG | 0.416189 | 4.3:-37351468 | MS.gene54573:CDS |
| GCAGCGCCACAAGTGGGTTC+AGG | 0.416212 | 4.3:+37355947 | None:intergenic |
| TCCCAGCATGAGAAACAGTA+TGG | 0.418225 | 4.3:+37356282 | None:intergenic |
| TTTGAAAACTATCCTCGCTC+TGG | 0.436202 | 4.3:-37356330 | MS.gene54573:CDS |
| GGGGAGAGCTGGGAGAGGAA+AGG | 0.438486 | 4.3:-37351223 | MS.gene54573:CDS |
| TTAGTGAGGGCTTTAGAGAA+TGG | 0.439616 | 4.3:-37351321 | MS.gene54573:CDS |
| GGCCCACCGCTCATAATAAT+GGG | 0.441437 | 4.3:+37354110 | None:intergenic |
| CTGAAATGGACGTATGCATA+TGG | 0.442573 | 4.3:-37351729 | MS.gene54573:CDS |
| TACTGAAAGAAGAAGGGAAA+TGG | 0.451324 | 4.3:-37354157 | MS.gene54573:CDS |
| GGTCGCTTGCATTTGGGACA+TGG | 0.453953 | 4.3:+37354784 | None:intergenic |
| TGTAGAAATCGTCATCTTCA+AGG | 0.456356 | 4.3:+37358065 | None:intergenic |
| CACTGGACGATGAGCCTCCT+CGG | 0.458483 | 4.3:+37355324 | None:intergenic |
| ATGATGATACTGAAAGAAGA+AGG | 0.463836 | 4.3:-37354164 | MS.gene54573:CDS |
| GGAGCAGTAAGGGGAGAGCT+GGG | 0.464600 | 4.3:-37351233 | MS.gene54573:CDS |
| ACATCCAGGACCATCATTGA+TGG | 0.478158 | 4.3:+37355985 | None:intergenic |
| AGCAGCTAAGCAAGAGGGAG+TGG | 0.478756 | 4.3:-37354278 | MS.gene54573:intron |
| TCCATACTGTTTCTCATGCT+GGG | 0.479243 | 4.3:-37356283 | MS.gene54573:CDS |
| AGTGTAGGAGAGTGCTGAAA+TGG | 0.479640 | 4.3:-37351743 | MS.gene54573:CDS |
| ATAAGATCTACGAAGGTAAC+GGG | 0.483111 | 4.3:+37355868 | None:intergenic |
| TCAAAGTGGTGTCCTGCCCC+TGG | 0.492940 | 4.3:-37355525 | MS.gene54573:CDS |
| CAGAGTCTGAAAACATGAAC+TGG | 0.493565 | 4.3:-37355261 | MS.gene54573:intron |
| GATACATACAGATTGGTTGC+TGG | 0.496836 | 4.3:+37354089 | None:intergenic |
| ATCTGATATCCAAGTAAGAC+AGG | 0.497385 | 4.3:-37357188 | MS.gene54573:intron |
| AGGTGAAGCAGAATCTGGTC+TGG | 0.499622 | 4.3:-37351565 | MS.gene54573:CDS |
| TATTACGGAGGCCTGGCTGC+AGG | 0.501896 | 4.3:-37351970 | MS.gene54573:intron |
| ACCAATAGCAGCGCCACAAG+TGG | 0.504223 | 4.3:+37355940 | None:intergenic |
| ATAAACTTAAGCTGTGACTC+AGG | 0.505087 | 4.3:+37351990 | None:intergenic |
| GGAAATGGCGAAGAATTCAT+TGG | 0.506032 | 4.3:-37354142 | MS.gene54573:CDS |
| TACTGTTTCTCATGCTGGGA+AGG | 0.514636 | 4.3:-37356279 | MS.gene54573:intron |
| GAAATTGAATTTACCAATTG+TGG | 0.519364 | 4.3:+37357095 | None:intergenic |
| TATCCACATAGGTTATGCCT+TGG | 0.520190 | 4.3:-37354358 | MS.gene54573:intron |
| AAATGCTGCAGGGAGCAGTA+AGG | 0.522523 | 4.3:-37351244 | MS.gene54573:CDS |
| CCTCGCTAGTATTAGGATGC+TGG | 0.523850 | 4.3:+37356486 | None:intergenic |
| AGCATCGTGCATATCATCTT+CGG | 0.523950 | 4.3:+37358102 | None:intergenic |
| TTGAAAACTATCCTCGCTCT+GGG | 0.528875 | 4.3:-37356329 | MS.gene54573:CDS |
| ACCACAGTGCCACAGTCCAC+TGG | 0.533681 | 4.3:+37355307 | None:intergenic |
| TTAAGTTTATTACGGAGGCC+TGG | 0.533852 | 4.3:-37351977 | MS.gene54573:CDS |
| CAGCAATTCCTCAGTGCTGA+AGG | 0.541611 | 4.3:-37351510 | MS.gene54573:CDS |
| CAAAATTAGCTGGATTGACT+AGG | 0.541759 | 4.3:-37351458 | MS.gene54573:intron |
| ACGTCCATCAATGATGGTCC+TGG | 0.548432 | 4.3:-37355989 | MS.gene54573:CDS |
| TGAGCTCGTTGCTTTGCTGA+AGG | 0.552429 | 4.3:+37351269 | None:intergenic |
| ATCAGCACGTCCATCAATGA+TGG | 0.552763 | 4.3:-37355995 | MS.gene54573:CDS |
| CAAATCATTTACCTGCAGCC+AGG | 0.554241 | 4.3:+37351959 | None:intergenic |
| ATAGGTTATGCCTTGGTGCA+TGG | 0.554256 | 4.3:-37354351 | MS.gene54573:intron |
| TGATGATACTGAAAGAAGAA+GGG | 0.556998 | 4.3:-37354163 | MS.gene54573:CDS |
| AATGCTGCAGGGAGCAGTAA+GGG | 0.560155 | 4.3:-37351243 | MS.gene54573:CDS |
| ACTTAAGCTGTGACTCAGGT+TGG | 0.561742 | 4.3:+37351994 | None:intergenic |
| GAACTTCTGATGATAGTCAC+TGG | 0.562651 | 4.3:-37351182 | MS.gene54573:CDS |
| CAGTTCTTTGAGTATTTGCA+AGG | 0.563576 | 4.3:-37351672 | MS.gene54573:intron |
| CTACTTCGAGAATTTAGTGA+GGG | 0.566132 | 4.3:-37351334 | MS.gene54573:CDS |
| AGTAAGGGGAGAGCTGGGAG+AGG | 0.566304 | 4.3:-37351228 | MS.gene54573:CDS |
| TGCTGTGGCCAGCTTACATG+TGG | 0.566414 | 4.3:-37356360 | MS.gene54573:intron |
| TCTTCCAGATAGTTGAGTGT+AGG | 0.573883 | 4.3:-37351758 | MS.gene54573:intron |
| GCATATTCACAACCAGGGGC+AGG | 0.573937 | 4.3:+37355513 | None:intergenic |
| CAGCAAAATTCAAATTTACA+GGG | 0.574826 | 4.3:+37354718 | None:intergenic |
| TCAGACCATGGTGAAAGAAC+TGG | 0.575256 | 4.3:-37354328 | MS.gene54573:CDS |
| GGGATTGGAATGGCTTCTTG+TGG | 0.575363 | 4.3:-37356309 | MS.gene54573:CDS |
| TCCTCGCTCTGGGATTGGAA+TGG | 0.579366 | 4.3:-37356319 | MS.gene54573:CDS |
| GTAGAAATCGTCATCTTCAA+GGG | 0.579796 | 4.3:+37358066 | None:intergenic |
| ACTGTGGCACTGTGGTAAAG+TGG | 0.587937 | 4.3:-37355300 | MS.gene54573:CDS |
| ACACCCATTATTATGAGCGG+TGG | 0.591402 | 4.3:-37354113 | MS.gene54573:CDS |
| TATAAGATCTACGAAGGTAA+CGG | 0.591928 | 4.3:+37355867 | None:intergenic |
| GAGCGAGTCCGAAAAGCGGT+TGG | 0.598853 | 4.3:-37356530 | MS.gene54573:CDS |
| CATCTCAACATCAAACATCC+AGG | 0.599803 | 4.3:+37355971 | None:intergenic |
| CCAATAGCAGCGCCACAAGT+GGG | 0.601504 | 4.3:+37355941 | None:intergenic |
| GTACTATTTACCTGAGCATG+AGG | 0.605750 | 4.3:-37351706 | MS.gene54573:CDS |
| ATGCTGCAGGGAGCAGTAAG+GGG | 0.605774 | 4.3:-37351242 | MS.gene54573:CDS |
| CACCCATTATTATGAGCGGT+GGG | 0.609091 | 4.3:-37354112 | MS.gene54573:CDS |
| TGAAATGGACGTATGCATAT+GGG | 0.610291 | 4.3:-37351728 | MS.gene54573:CDS |
| AGATGTCCTGAACCCACTTG+TGG | 0.620859 | 4.3:-37355953 | MS.gene54573:CDS |
| CAAAATTCAAATTTACAGGG+TGG | 0.621414 | 4.3:+37354721 | None:intergenic |
| TTATGAAGCAGCTAAGCAAG+AGG | 0.623330 | 4.3:-37354284 | MS.gene54573:CDS |
| CGAGGAGGCTCATCGTCCAG+TGG | 0.625879 | 4.3:-37355323 | MS.gene54573:CDS |
| ATCTGATGTAGATGCTAATG+GGG | 0.630234 | 4.3:-37351295 | MS.gene54573:CDS |
| GAAGATGACGATTTCTACAG+TGG | 0.633360 | 4.3:-37358061 | MS.gene54573:intron |
| TATGAAGCAGCTAAGCAAGA+GGG | 0.635282 | 4.3:-37354283 | MS.gene54573:CDS |
| TGAAGAGCGAGTCCGAAAAG+CGG | 0.637498 | 4.3:-37356534 | MS.gene54573:CDS |
| GCCATTCCAATCCCAGAGCG+AGG | 0.643551 | 4.3:+37356318 | None:intergenic |
| GACCATGGTGAAAGAACTGG+TGG | 0.643713 | 4.3:-37354325 | MS.gene54573:CDS |
| ACAGCTTAAGTTTATTACGG+AGG | 0.646409 | 4.3:-37351982 | MS.gene54573:CDS |
| CTTCGATATAAGATCTACGA+AGG | 0.648378 | 4.3:+37355861 | None:intergenic |
| GCTCATCGTCCAGTGGACTG+TGG | 0.650054 | 4.3:-37355316 | MS.gene54573:CDS |
| TACTATTTACCTGAGCATGA+GGG | 0.652156 | 4.3:-37351705 | MS.gene54573:CDS |
| ACTACTTCGAGAATTTAGTG+AGG | 0.655176 | 4.3:-37351335 | MS.gene54573:CDS |
| GTAACTGCATATTCACAACC+AGG | 0.655441 | 4.3:+37355507 | None:intergenic |
| AACAAAAGCTATACGAACAG+AGG | 0.655471 | 4.3:+37355448 | None:intergenic |
| AACTGCATATTCACAACCAG+GGG | 0.659172 | 4.3:+37355509 | None:intergenic |
| ATTGCAAATCTGGCATGTGG+TGG | 0.660392 | 4.3:+37351122 | None:intergenic |
| AACCACCAGTTCTTTCACCA+TGG | 0.660461 | 4.3:+37354323 | None:intergenic |
| TCAGTAAAGTGCATGATGCA+TGG | 0.665829 | 4.3:-37356565 | MS.gene54573:CDS |
| TAACTGCATATTCACAACCA+GGG | 0.669788 | 4.3:+37355508 | None:intergenic |
| TAGATTGGCCTTCAGCACTG+AGG | 0.685503 | 4.3:+37351502 | None:intergenic |
| GATACACCCATTATTATGAG+CGG | 0.686220 | 4.3:-37354116 | MS.gene54573:CDS |
| TTGATTGCAAATCTGGCATG+TGG | 0.691443 | 4.3:+37351119 | None:intergenic |
| CCAGCATCCTAATACTAGCG+AGG | 0.692364 | 4.3:-37356486 | MS.gene54573:intron |
| GAATCTACAGTGCACCGAGG+AGG | 0.695123 | 4.3:-37355338 | MS.gene54573:intron |
| CATGGTCTGACCATGCACCA+AGG | 0.699416 | 4.3:+37354341 | None:intergenic |
| CTTGGTGCATGGTCAGACCA+TGG | 0.701151 | 4.3:-37354340 | MS.gene54573:CDS |
| TCCAGTGGACTGTGGCACTG+TGG | 0.734400 | 4.3:-37355308 | MS.gene54573:CDS |
| GGCAGATTTGACATTCACAG+AGG | 0.749917 | 4.3:+37351143 | None:intergenic |
| ACTGAATCTACAGTGCACCG+AGG | 0.790165 | 4.3:-37355341 | MS.gene54573:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 37351110 | 37358131 | 37351110 | ID=MS.gene54573 |
| chr4.3 | mRNA | 37351110 | 37358131 | 37351110 | ID=MS.gene54573.t1;Parent=MS.gene54573 |
| chr4.3 | exon | 37358062 | 37358131 | 37358062 | ID=MS.gene54573.t1.exon1;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37358062 | 37358131 | 37358062 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37357109 | 37357196 | 37357109 | ID=MS.gene54573.t1.exon2;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37357109 | 37357196 | 37357109 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37356487 | 37356592 | 37356487 | ID=MS.gene54573.t1.exon3;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37356487 | 37356592 | 37356487 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37356280 | 37356370 | 37356280 | ID=MS.gene54573.t1.exon4;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37356280 | 37356370 | 37356280 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37355850 | 37356022 | 37355850 | ID=MS.gene54573.t1.exon5;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37355850 | 37356022 | 37355850 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37355443 | 37355547 | 37355443 | ID=MS.gene54573.t1.exon6;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37355443 | 37355547 | 37355443 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37355262 | 37355350 | 37355262 | ID=MS.gene54573.t1.exon7;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37355262 | 37355350 | 37355262 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37354718 | 37354825 | 37354718 | ID=MS.gene54573.t1.exon8;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37354718 | 37354825 | 37354718 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37354279 | 37354369 | 37354279 | ID=MS.gene54573.t1.exon9;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37354279 | 37354369 | 37354279 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37354098 | 37354187 | 37354098 | ID=MS.gene54573.t1.exon10;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37354098 | 37354187 | 37354098 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37352348 | 37352395 | 37352348 | ID=MS.gene54573.t1.exon11;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37352348 | 37352395 | 37352348 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37351971 | 37352042 | 37351971 | ID=MS.gene54573.t1.exon12;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37351971 | 37352042 | 37351971 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37351673 | 37351772 | 37351673 | ID=MS.gene54573.t1.exon13;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37351673 | 37351772 | 37351673 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37351459 | 37351585 | 37351459 | ID=MS.gene54573.t1.exon14;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37351459 | 37351585 | 37351459 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| chr4.3 | exon | 37351110 | 37351368 | 37351110 | ID=MS.gene54573.t1.exon15;Parent=MS.gene54573.t1 |
| chr4.3 | CDS | 37351110 | 37351368 | 37351110 | ID=cds.MS.gene54573.t1;Parent=MS.gene54573.t1 |
| Gene Sequence |
| Protein sequence |
