AlfalfaGEDB | MS.gene63280

M. sativa cultivar XinJiangDaYe / MS.gene63280

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0008150 GO:0008152 GO:0009521 GO:0009523 GO:0009654 GO:0009987 GO:0015979 GO:0019898 GO:0032991 GO:0043167 GO:0043169 GO:0044237 GO:0046872 GO:0098796 GO:0110165 GO:1902494 GO:1990204
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63280.t1	XP_003600596.2	97.6	127	3	0	1	127	51	177	3.30E-61	244.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63280.t1	Q2V4B2	44.5	128	65	3	6	127	60	187	3.7e-21	102.4

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene63280.t1	G7J7V3	97.6	127	3	0	1	127	51	177	2.4e-61	244.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049346	MS.gene63280	0.803065	4.16E-49	-1.69E-46
MS.gene049351	MS.gene63280	0.831854	1.32E-55	-1.69E-46
MS.gene049427	MS.gene63280	0.824398	8.25E-54	-1.69E-46
MS.gene049661	MS.gene63280	0.987052	6.91E-169	-1.69E-46
MS.gene049662	MS.gene63280	0.995367	1.45E-215	-1.69E-46
MS.gene049758	MS.gene63280	0.826228	3.04E-54	-1.69E-46
MS.gene050076	MS.gene63280	0.839424	1.60E-57	-1.69E-46
MS.gene050301	MS.gene63280	0.854859	9.30E-62	-1.69E-46
MS.gene050397	MS.gene63280	0.84409	9.38E-59	-1.69E-46
MS.gene050406	MS.gene63280	0.806896	6.58E-50	-1.69E-46
MS.gene050432	MS.gene63280	0.847656	1.01E-59	-1.69E-46
MS.gene050464	MS.gene63280	0.80655	7.79E-50	-1.69E-46
MS.gene050529	MS.gene63280	0.836504	9.00E-57	-1.69E-46
MS.gene05053	MS.gene63280	0.827367	1.63E-54	-1.69E-46
MS.gene050670	MS.gene63280	0.819307	1.24E-52	-1.69E-46
MS.gene050767	MS.gene63280	0.8199	9.11E-53	-1.69E-46
MS.gene050770	MS.gene63280	0.821571	3.76E-53	-1.69E-46
MS.gene05101	MS.gene63280	0.851458	8.76E-61	-1.69E-46
MS.gene05105	MS.gene63280	0.854874	9.20E-62	-1.69E-46
MS.gene051471	MS.gene63280	0.83282	7.60E-56	-1.69E-46
MS.gene051692	MS.gene63280	0.807367	5.23E-50	-1.69E-46
MS.gene051877	MS.gene63280	0.83157	1.55E-55	-1.69E-46
MS.gene052078	MS.gene63280	0.840563	8.07E-58	-1.69E-46
MS.gene052178	MS.gene63280	0.808379	3.19E-50	-1.69E-46
MS.gene052188	MS.gene63280	0.833308	5.75E-56	-1.69E-46
MS.gene052253	MS.gene63280	0.843188	1.63E-58	-1.69E-46
MS.gene052290	MS.gene63280	0.81232	4.50E-51	-1.69E-46
MS.gene052310	MS.gene63280	0.865789	4.58E-65	-1.69E-46
MS.gene052322	MS.gene63280	0.814362	1.60E-51	-1.69E-46
MS.gene052326	MS.gene63280	0.822027	2.95E-53	-1.69E-46
MS.gene052340	MS.gene63280	0.836766	7.72E-57	-1.69E-46
MS.gene052421	MS.gene63280	0.819516	1.11E-52	-1.69E-46
MS.gene052481	MS.gene63280	0.80574	1.15E-49	-1.69E-46
MS.gene052485	MS.gene63280	0.815591	8.55E-52	-1.69E-46
MS.gene053103	MS.gene63280	0.811846	5.71E-51	-1.69E-46
MS.gene053209	MS.gene63280	0.833533	5.05E-56	-1.69E-46
MS.gene053322	MS.gene63280	0.819774	9.73E-53	-1.69E-46
MS.gene053557	MS.gene63280	0.815974	7.02E-52	-1.69E-46
MS.gene053758	MS.gene63280	0.807816	4.20E-50	-1.69E-46
MS.gene053836	MS.gene63280	0.83992	1.19E-57	-1.69E-46
MS.gene054123	MS.gene63280	0.825346	4.93E-54	-1.69E-46
MS.gene054376	MS.gene63280	0.834724	2.54E-56	-1.69E-46
MS.gene05455	MS.gene63280	0.803833	2.89E-49	-1.69E-46
MS.gene054802	MS.gene63280	0.82864	8.04E-55	-1.69E-46
MS.gene054803	MS.gene63280	0.830055	3.64E-55	-1.69E-46
MS.gene054804	MS.gene63280	0.829956	3.85E-55	-1.69E-46
MS.gene05505	MS.gene63280	0.812472	4.17E-51	-1.69E-46
MS.gene055116	MS.gene63280	0.879459	1.23E-69	-1.69E-46
MS.gene055254	MS.gene63280	0.813468	2.52E-51	-1.69E-46
MS.gene055277	MS.gene63280	0.874233	7.92E-68	-1.69E-46
MS.gene055390	MS.gene63280	0.81301	3.18E-51	-1.69E-46
MS.gene055397	MS.gene63280	0.804307	2.30E-49	-1.69E-46
MS.gene055422	MS.gene63280	0.844661	6.59E-59	-1.69E-46
MS.gene055428	MS.gene63280	0.8463	2.37E-59	-1.69E-46
MS.gene055499	MS.gene63280	0.830248	3.27E-55	-1.69E-46
MS.gene055604	MS.gene63280	0.801639	8.19E-49	-1.69E-46
MS.gene056191	MS.gene63280	0.849714	2.71E-60	-1.69E-46
MS.gene056407	MS.gene63280	0.827177	1.81E-54	-1.69E-46
MS.gene056410	MS.gene63280	0.823065	1.69E-53	-1.69E-46
MS.gene056511	MS.gene63280	0.828978	6.66E-55	-1.69E-46
MS.gene056609	MS.gene63280	0.824466	7.95E-54	-1.69E-46
MS.gene056612	MS.gene63280	0.844413	7.68E-59	-1.69E-46
MS.gene057011	MS.gene63280	0.801694	7.98E-49	-1.69E-46
MS.gene05702	MS.gene63280	0.871191	8.25E-67	-1.69E-46
MS.gene057123	MS.gene63280	0.816904	4.35E-52	-1.69E-46
MS.gene057124	MS.gene63280	0.807857	4.12E-50	-1.69E-46
MS.gene057129	MS.gene63280	0.82095	5.23E-53	-1.69E-46
MS.gene057146	MS.gene63280	0.811794	5.86E-51	-1.69E-46
MS.gene057245	MS.gene63280	0.803447	3.47E-49	-1.69E-46
MS.gene057295	MS.gene63280	0.803617	3.20E-49	-1.69E-46
MS.gene05733	MS.gene63280	0.83457	2.78E-56	-1.69E-46
MS.gene057448	MS.gene63280	0.80097	1.12E-48	-1.69E-46
MS.gene057450	MS.gene63280	0.804937	1.70E-49	-1.69E-46
MS.gene057749	MS.gene63280	0.814153	1.78E-51	-1.69E-46
MS.gene057782	MS.gene63280	0.8134	2.61E-51	-1.69E-46
MS.gene057823	MS.gene63280	0.808211	3.46E-50	-1.69E-46
MS.gene058002	MS.gene63280	0.833164	6.24E-56	-1.69E-46
MS.gene058082	MS.gene63280	0.808773	2.63E-50	-1.69E-46
MS.gene05811	MS.gene63280	0.822373	2.45E-53	-1.69E-46
MS.gene058233	MS.gene63280	0.834784	2.45E-56	-1.69E-46
MS.gene058293	MS.gene63280	0.826625	2.45E-54	-1.69E-46
MS.gene058365	MS.gene63280	0.806946	6.43E-50	-1.69E-46
MS.gene058660	MS.gene63280	0.882545	9.53E-71	-1.69E-46
MS.gene058787	MS.gene63280	0.822123	2.80E-53	-1.69E-46
MS.gene058928	MS.gene63280	0.834871	2.33E-56	-1.69E-46
MS.gene05907	MS.gene63280	0.842835	2.03E-58	-1.69E-46
MS.gene059141	MS.gene63280	0.80214	6.46E-49	-1.69E-46
MS.gene059294	MS.gene63280	0.82089	5.40E-53	-1.69E-46
MS.gene059536	MS.gene63280	0.834993	2.17E-56	-1.69E-46
MS.gene059597	MS.gene63280	0.837545	4.88E-57	-1.69E-46
MS.gene059676	MS.gene63280	0.811384	7.19E-51	-1.69E-46
MS.gene059688	MS.gene63280	0.874728	5.38E-68	-1.69E-46
MS.gene059692	MS.gene63280	0.876373	1.47E-68	-1.69E-46
MS.gene059695	MS.gene63280	0.870039	1.97E-66	-1.69E-46
MS.gene05984	MS.gene63280	0.808938	2.42E-50	-1.69E-46
MS.gene059894	MS.gene63280	0.827804	1.28E-54	-1.69E-46
MS.gene059960	MS.gene63280	0.811427	7.04E-51	-1.69E-46
MS.gene06037	MS.gene63280	0.854797	9.69E-62	-1.69E-46
MS.gene060403	MS.gene63280	0.814376	1.59E-51	-1.69E-46
MS.gene060804	MS.gene63280	0.840806	6.97E-58	-1.69E-46
MS.gene061041	MS.gene63280	0.829299	5.56E-55	-1.69E-46
MS.gene061049	MS.gene63280	0.882183	1.29E-70	-1.69E-46
MS.gene061051	MS.gene63280	-0.840271	9.61E-58	-1.69E-46
MS.gene061200	MS.gene63280	0.816939	4.27E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 15 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATTGCAAATGCATTTGATTT+TGG	0.133260	3.3:-60679806	MS.gene63280:CDS
AACTGTGACCTTTCACTTCC+AGG	0.232695	3.3:+60679605	None:intergenic
GCCTCTTCAACTGTTTGATC+AGG	0.315431	3.3:+60679773	None:intergenic
TCATAACGCTGCCACACTTC+AGG	0.379368	3.3:+60679391	None:intergenic
GTCATGGAAAATAGCACAAA+AGG	0.423780	3.3:-60679687	MS.gene63280:CDS
TTAGTGGAGAGTGCATGTTT+AGG	0.480583	3.3:-60679835	None:intergenic
ACCATAATTCAAAACAAGCC+TGG	0.511434	3.3:-60679623	MS.gene63280:CDS
GAAGTGAAAGGTCACAGTTA+AGG	0.517020	3.3:-60679601	MS.gene63280:CDS
CCTCTTCAACTGTTTGATCA+GGG	0.547039	3.3:+60679774	None:intergenic
CCCTGATCAAACAGTTGAAG+AGG	0.549247	3.3:-60679774	MS.gene63280:CDS
AAAACAAGCCTGGAAGTGAA+AGG	0.596015	3.3:-60679613	MS.gene63280:CDS
ATTTGTTAGAGTCAAAGTCA+TGG	0.608950	3.3:-60679703	MS.gene63280:CDS
ATCAAGATGGACCTGAAGTG+TGG	0.634230	3.3:-60679402	MS.gene63280:CDS
GCGTTATGAGAACATTGTTG+TGG	0.635212	3.3:-60679377	MS.gene63280:CDS
TATGCAGCAAGAGATCAAGA+TGG	0.661307	3.3:-60679415	MS.gene63280:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAATTCTATCAAGAATATAA+TGG	-	chr3.3:60679834-60679853	MS.gene63280:CDS	15.0%
!!!	TTTATTGTTTTTGTTGTGTA+AGG	-	chr3.3:60679682-60679701	MS.gene63280:CDS	20.0%
!!!	ATTGATGTTTAGTTTTGCAT+TGG	-	chr3.3:60679720-60679739	MS.gene63280:CDS	25.0%
!!!	ATTGCAAATGCATTTGATTT+TGG	-	chr3.3:60679373-60679392	MS.gene63280:CDS	25.0%
	TCAACAATGTTACTAGAGTA+AGG	-	chr3.3:60679620-60679639	MS.gene63280:CDS	30.0%
!	ATTTGTTAGAGTCAAAGTCA+TGG	-	chr3.3:60679476-60679495	MS.gene63280:intron	30.0%
	ACCATAATTCAAAACAAGCC+TGG	-	chr3.3:60679556-60679575	MS.gene63280:intron	35.0%
	GTCATGGAAAATAGCACAAA+AGG	-	chr3.3:60679492-60679511	MS.gene63280:intron	35.0%
!!	AAGTTCAGCACTTTTGAAGA+AGG	-	chr3.3:60679525-60679544	MS.gene63280:intron	35.0%
!!	TGACAAAGTGTCCTAGTTTT+AGG	+	chr3.3:60679655-60679674	None:intergenic	35.0%
!!!	TCCAGGCTTGTTTTGAATTA+TGG	+	chr3.3:60679560-60679579	None:intergenic	35.0%
	AAAACAAGCCTGGAAGTGAA+AGG	-	chr3.3:60679566-60679585	MS.gene63280:CDS	40.0%
	CCTCTTCAACTGTTTGATCA+GGG	+	chr3.3:60679408-60679427	None:intergenic	40.0%
	GAAGTGAAAGGTCACAGTTA+AGG	-	chr3.3:60679578-60679597	MS.gene63280:CDS	40.0%
	GCGTTATGAGAACATTGTTG+TGG	-	chr3.3:60679802-60679821	MS.gene63280:CDS	40.0%
	GGAGACTTTGTCCTAAAACT+AGG	-	chr3.3:60679641-60679660	MS.gene63280:CDS	40.0%
	TATGCAGCAAGAGATCAAGA+TGG	-	chr3.3:60679764-60679783	MS.gene63280:CDS	40.0%
	AACTGTGACCTTTCACTTCC+AGG	+	chr3.3:60679577-60679596	None:intergenic	45.0%
	ATCAAGATGGACCTGAAGTG+TGG	-	chr3.3:60679777-60679796	MS.gene63280:CDS	45.0%
	CCCTGATCAAACAGTTGAAG+AGG	-	chr3.3:60679405-60679424	MS.gene63280:CDS	45.0%
	GCCTCTTCAACTGTTTGATC+AGG	+	chr3.3:60679409-60679428	None:intergenic	45.0%
	TCATAACGCTGCCACACTTC+AGG	+	chr3.3:60679791-60679810	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	60679348	60679853	60679348	ID=MS.gene63280
chr3.3	mRNA	60679348	60679853	60679348	ID=MS.gene63280.t1;Parent=MS.gene63280
chr3.3	exon	60679565	60679853	60679565	ID=MS.gene63280.t1.exon1;Parent=MS.gene63280.t1
chr3.3	CDS	60679565	60679853	60679565	ID=cds.MS.gene63280.t1;Parent=MS.gene63280.t1
chr3.3	exon	60679348	60679443	60679348	ID=MS.gene63280.t1.exon2;Parent=MS.gene63280.t1
chr3.3	CDS	60679348	60679443	60679348	ID=cds.MS.gene63280.t1;Parent=MS.gene63280.t1

Gene Sequence

>MS.gene63280

TGGAGAGTGCATGTTTAGGACAAAGATTGCAAATGCATTTGATTTTGGACTTGTTGTCCCTGATCAAACAGTTGAAGAGGCACAAAATGTAGTGAGAGTTCATGCACAAGATTTATTGCAAGTTAGAGATTTGTTAGAGTCAAAGTCATGGAAAATAGCACAAAAGGAATTGAGAAGAAGTTCAGCACTTTTGAAGAAGGATATATACACCATAATTCAAAACAAGCCTGGAAGTGAAAGGTCACAGTTAAGGAAGCTCTATTCTACTCTCTTCAACAATGTTACTAGAGTAAGGAGACTTTGTCCTAAAACTAGGACACTTTGTCATGAAGAGTTTATTGTTTTTGTTGTGTAAGGTTTTATTACTTTTCAATTGATGTTTAGTTTTGCATTGGTGAAAAATAACGCAGCTAGATTATGCAGCAAGAGATCAAGATGGACCTGAAGTGTGGCAGCGTTATGAGAACATTGTTGTGGCTGTGAATGAAATTCTATCAAGAATATAA

Protein sequence

>MS.gene63280.t1

WRVHVUDKDCKCIUFWTCCPUSNSURGTKCSESSCTRFIASURFVRVKVMENSTKGIEKKFSTFEEGYIHHNSKQAWKUKVTVKEALFYSLQQCYUTRLCSKRSRWTUSVAALUEHCCGCEUNSIKNI