Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69110.t1 | GAU14324.1 | 85.2 | 88 | 11 | 2 | 4 | 90 | 1 | 87 | 5.90E-33 | 149.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69110.t1 | G7J4L6 | 85.1 | 87 | 7 | 1 | 4 | 90 | 1 | 81 | 2.8e-32 | 147.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049346 | MS.gene69110 | 0.829685 | 4.48E-55 | -1.69E-46 |
| MS.gene049356 | MS.gene69110 | 0.828756 | 7.53E-55 | -1.69E-46 |
| MS.gene049488 | MS.gene69110 | 0.840685 | 7.49E-58 | -1.69E-46 |
| MS.gene049661 | MS.gene69110 | 0.81487 | 1.24E-51 | -1.69E-46 |
| MS.gene049662 | MS.gene69110 | 0.802067 | 6.69E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene69110 | 0.803852 | 2.86E-49 | -1.69E-46 |
| MS.gene049910 | MS.gene69110 | 0.820868 | 5.46E-53 | -1.69E-46 |
| MS.gene050060 | MS.gene69110 | 0.849764 | 2.62E-60 | -1.69E-46 |
| MS.gene050301 | MS.gene69110 | 0.828353 | 9.42E-55 | -1.69E-46 |
| MS.gene050397 | MS.gene69110 | 0.80374 | 3.02E-49 | -1.69E-46 |
| MS.gene050406 | MS.gene69110 | 0.836747 | 7.81E-57 | -1.69E-46 |
| MS.gene050426 | MS.gene69110 | -0.817394 | 3.37E-52 | -1.69E-46 |
| MS.gene050464 | MS.gene69110 | 0.854516 | 1.17E-61 | -1.69E-46 |
| MS.gene050529 | MS.gene69110 | 0.847868 | 8.80E-60 | -1.69E-46 |
| MS.gene050670 | MS.gene69110 | 0.871212 | 8.12E-67 | -1.69E-46 |
| MS.gene05105 | MS.gene69110 | 0.846412 | 2.21E-59 | -1.69E-46 |
| MS.gene051525 | MS.gene69110 | 0.815239 | 1.02E-51 | -1.69E-46 |
| MS.gene051582 | MS.gene69110 | 0.813065 | 3.09E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene69110 | 0.810173 | 1.31E-50 | -1.69E-46 |
| MS.gene051983 | MS.gene69110 | 0.802416 | 5.67E-49 | -1.69E-46 |
| MS.gene052011 | MS.gene69110 | -0.822285 | 2.57E-53 | -1.69E-46 |
| MS.gene052077 | MS.gene69110 | 0.856533 | 3.02E-62 | -1.69E-46 |
| MS.gene052078 | MS.gene69110 | 0.83492 | 2.27E-56 | -1.69E-46 |
| MS.gene052134 | MS.gene69110 | 0.807747 | 4.35E-50 | -1.69E-46 |
| MS.gene052135 | MS.gene69110 | 0.816648 | 4.96E-52 | -1.69E-46 |
| MS.gene052136 | MS.gene69110 | 0.80048 | 1.41E-48 | -1.69E-46 |
| MS.gene052188 | MS.gene69110 | 0.879182 | 1.54E-69 | -1.69E-46 |
| MS.gene052253 | MS.gene69110 | 0.869621 | 2.70E-66 | -1.69E-46 |
| MS.gene052310 | MS.gene69110 | 0.846721 | 1.82E-59 | -1.69E-46 |
| MS.gene052340 | MS.gene69110 | 0.872386 | 3.31E-67 | -1.69E-46 |
| MS.gene052421 | MS.gene69110 | 0.809773 | 1.60E-50 | -1.69E-46 |
| MS.gene052481 | MS.gene69110 | 0.826813 | 2.21E-54 | -1.69E-46 |
| MS.gene052485 | MS.gene69110 | 0.838187 | 3.34E-57 | -1.69E-46 |
| MS.gene052758 | MS.gene69110 | 0.804236 | 2.38E-49 | -1.69E-46 |
| MS.gene053073 | MS.gene69110 | 0.805714 | 1.17E-49 | -1.69E-46 |
| MS.gene053103 | MS.gene69110 | 0.833495 | 5.16E-56 | -1.69E-46 |
| MS.gene053209 | MS.gene69110 | 0.818281 | 2.13E-52 | -1.69E-46 |
| MS.gene053449 | MS.gene69110 | 0.814084 | 1.84E-51 | -1.69E-46 |
| MS.gene053557 | MS.gene69110 | 0.811952 | 5.41E-51 | -1.69E-46 |
| MS.gene053836 | MS.gene69110 | 0.839379 | 1.64E-57 | -1.69E-46 |
| MS.gene053912 | MS.gene69110 | 0.82771 | 1.35E-54 | -1.69E-46 |
| MS.gene053928 | MS.gene69110 | 0.80479 | 1.82E-49 | -1.69E-46 |
| MS.gene054183 | MS.gene69110 | 0.833046 | 6.68E-56 | -1.69E-46 |
| MS.gene054349 | MS.gene69110 | 0.850681 | 1.45E-60 | -1.69E-46 |
| MS.gene054473 | MS.gene69110 | 0.807784 | 4.27E-50 | -1.69E-46 |
| MS.gene054719 | MS.gene69110 | 0.810434 | 1.15E-50 | -1.69E-46 |
| MS.gene054930 | MS.gene69110 | 0.813948 | 1.98E-51 | -1.69E-46 |
| MS.gene054955 | MS.gene69110 | 0.809752 | 1.62E-50 | -1.69E-46 |
| MS.gene054983 | MS.gene69110 | 0.804214 | 2.40E-49 | -1.69E-46 |
| MS.gene055116 | MS.gene69110 | 0.871605 | 6.02E-67 | -1.69E-46 |
| MS.gene055422 | MS.gene69110 | 0.821622 | 3.66E-53 | -1.69E-46 |
| MS.gene055428 | MS.gene69110 | 0.818941 | 1.51E-52 | -1.69E-46 |
| MS.gene05553 | MS.gene69110 | 0.834268 | 3.31E-56 | -1.69E-46 |
| MS.gene055601 | MS.gene69110 | -0.810571 | 1.08E-50 | -1.69E-46 |
| MS.gene056191 | MS.gene69110 | 0.809082 | 2.26E-50 | -1.69E-46 |
| MS.gene056407 | MS.gene69110 | 0.836777 | 7.67E-57 | -1.69E-46 |
| MS.gene056511 | MS.gene69110 | 0.83305 | 6.66E-56 | -1.69E-46 |
| MS.gene056659 | MS.gene69110 | 0.8395 | 1.53E-57 | -1.69E-46 |
| MS.gene056719 | MS.gene69110 | 0.805533 | 1.27E-49 | -1.69E-46 |
| MS.gene056899 | MS.gene69110 | -0.854555 | 1.14E-61 | -1.69E-46 |
| MS.gene05702 | MS.gene69110 | 0.864437 | 1.22E-64 | -1.69E-46 |
| MS.gene057123 | MS.gene69110 | 0.80265 | 5.07E-49 | -1.69E-46 |
| MS.gene057124 | MS.gene69110 | 0.827009 | 1.98E-54 | -1.69E-46 |
| MS.gene057146 | MS.gene69110 | 0.807973 | 3.89E-50 | -1.69E-46 |
| MS.gene057396 | MS.gene69110 | 0.820388 | 7.04E-53 | -1.69E-46 |
| MS.gene057448 | MS.gene69110 | 0.828612 | 8.16E-55 | -1.69E-46 |
| MS.gene057449 | MS.gene69110 | 0.81211 | 5.00E-51 | -1.69E-46 |
| MS.gene057450 | MS.gene69110 | 0.830051 | 3.65E-55 | -1.69E-46 |
| MS.gene057476 | MS.gene69110 | 0.800439 | 1.44E-48 | -1.69E-46 |
| MS.gene057749 | MS.gene69110 | 0.871234 | 7.98E-67 | -1.69E-46 |
| MS.gene057823 | MS.gene69110 | 0.876639 | 1.19E-68 | -1.69E-46 |
| MS.gene057825 | MS.gene69110 | 0.811364 | 7.26E-51 | -1.69E-46 |
| MS.gene058002 | MS.gene69110 | 0.838338 | 3.05E-57 | -1.69E-46 |
| MS.gene05811 | MS.gene69110 | 0.810791 | 9.67E-51 | -1.69E-46 |
| MS.gene058142 | MS.gene69110 | 0.81914 | 1.36E-52 | -1.69E-46 |
| MS.gene058233 | MS.gene69110 | 0.800267 | 1.56E-48 | -1.69E-46 |
| MS.gene058293 | MS.gene69110 | 0.804252 | 2.36E-49 | -1.69E-46 |
| MS.gene058365 | MS.gene69110 | 0.802833 | 4.65E-49 | -1.69E-46 |
| MS.gene058660 | MS.gene69110 | 0.817452 | 3.27E-52 | -1.69E-46 |
| MS.gene058710 | MS.gene69110 | 0.82609 | 3.28E-54 | -1.69E-46 |
| MS.gene05886 | MS.gene69110 | 0.856536 | 3.01E-62 | -1.69E-46 |
| MS.gene058928 | MS.gene69110 | 0.87032 | 1.59E-66 | -1.69E-46 |
| MS.gene059011 | MS.gene69110 | 0.820883 | 5.42E-53 | -1.69E-46 |
| MS.gene05907 | MS.gene69110 | 0.835153 | 1.98E-56 | -1.69E-46 |
| MS.gene059141 | MS.gene69110 | 0.81659 | 5.11E-52 | -1.69E-46 |
| MS.gene059285 | MS.gene69110 | 0.876455 | 1.38E-68 | -1.69E-46 |
| MS.gene059294 | MS.gene69110 | 0.800477 | 1.41E-48 | -1.69E-46 |
| MS.gene059366 | MS.gene69110 | 0.862061 | 6.61E-64 | -1.69E-46 |
| MS.gene059420 | MS.gene69110 | 0.869125 | 3.91E-66 | -1.69E-46 |
| MS.gene059455 | MS.gene69110 | 0.806005 | 1.01E-49 | -1.69E-46 |
| MS.gene059597 | MS.gene69110 | 0.861952 | 7.14E-64 | -1.69E-46 |
| MS.gene059676 | MS.gene69110 | 0.828942 | 6.79E-55 | -1.69E-46 |
| MS.gene059688 | MS.gene69110 | 0.868062 | 8.63E-66 | -1.69E-46 |
| MS.gene059692 | MS.gene69110 | 0.867624 | 1.19E-65 | -1.69E-46 |
| MS.gene059695 | MS.gene69110 | 0.863875 | 1.82E-64 | -1.69E-46 |
| MS.gene059807 | MS.gene69110 | 0.814942 | 1.19E-51 | -1.69E-46 |
| MS.gene05984 | MS.gene69110 | 0.830212 | 3.33E-55 | -1.69E-46 |
| MS.gene059894 | MS.gene69110 | 0.844472 | 7.40E-59 | -1.69E-46 |
| MS.gene060247 | MS.gene69110 | 0.83113 | 1.99E-55 | -1.69E-46 |
| MS.gene06037 | MS.gene69110 | 0.817208 | 3.71E-52 | -1.69E-46 |
| MS.gene060403 | MS.gene69110 | 0.850832 | 1.32E-60 | -1.69E-46 |
| MS.gene060407 | MS.gene69110 | 0.813067 | 3.09E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene69110 | 0.891518 | 3.69E-74 | -1.69E-46 |
| MS.gene061051 | MS.gene69110 | -0.814732 | 1.33E-51 | -1.69E-46 |
| MS.gene061154 | MS.gene69110 | 0.80736 | 5.25E-50 | -1.69E-46 |
| MS.gene061159 | MS.gene69110 | 0.847811 | 9.13E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69110.t1 | MTR_3g111360 | 85.057 | 87 | 7 | 1 | 4 | 90 | 1 | 81 | 5.34e-47 | 145 |
| MS.gene69110.t1 | MTR_5g067050 | 65.714 | 70 | 20 | 3 | 22 | 89 | 13 | 80 | 1.47e-23 | 85.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAGGGTTGCTTTCATGCTT+TGG | 0.283837 | 3.4:-96313446 | MS.gene69110:CDS |
| TTAGCTAGCAATGGAAAGTT+TGG | 0.296785 | 3.4:-96313510 | None:intergenic |
| GAGGGTTGCTTTCATGCTTT+GGG | 0.333399 | 3.4:-96313445 | MS.gene69110:CDS |
| TTGTTTGTTCTCCTCTTCCA+TGG | 0.436490 | 3.4:+96313404 | None:intergenic |
| GAGGACGACGAAGAATCTTT+AGG | 0.441691 | 3.4:-96313305 | MS.gene69110:CDS |
| TCTCCTATAAGAAGCAAAAC+AGG | 0.462208 | 3.4:-96313473 | MS.gene69110:CDS |
| AGAAGCAAAACAGGTGGAGG+AGG | 0.521376 | 3.4:-96313464 | MS.gene69110:CDS |
| TGATGATGGTAATTTGGAGG+AGG | 0.522189 | 3.4:-96313324 | MS.gene69110:CDS |
| AACACTTATAGAACCGTTGG+TGG | 0.530511 | 3.4:-96313383 | MS.gene69110:CDS |
| AATTGAAGCTTCCATGGAAG+AGG | 0.536567 | 3.4:-96313415 | MS.gene69110:CDS |
| AACAACACTTATAGAACCGT+TGG | 0.561342 | 3.4:-96313386 | MS.gene69110:CDS |
| GTCTCAAATTGAAGCTTCCA+TGG | 0.569556 | 3.4:-96313421 | MS.gene69110:CDS |
| ATAAGAAGCAAAACAGGTGG+AGG | 0.576069 | 3.4:-96313467 | MS.gene69110:CDS |
| GACGAAGAATCTTTAGGTCG+CGG | 0.579606 | 3.4:-96313299 | MS.gene69110:CDS |
| ATCATACCTAAAACCACCAA+CGG | 0.621720 | 3.4:+96313370 | None:intergenic |
| TTGCTTCTTATAGGAGACGA+TGG | 0.630393 | 3.4:+96313479 | None:intergenic |
| GAAGCAAAACAGGTGGAGGA+GGG | 0.645020 | 3.4:-96313463 | MS.gene69110:CDS |
| CCTATAAGAAGCAAAACAGG+TGG | 0.657762 | 3.4:-96313470 | MS.gene69110:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATTTTGATGATGGTAATT+TGG | - | chr3.4:96313420-96313439 | MS.gene69110:CDS | 20.0% |
| !! | AAATTTTGAGCATAGCTTAA+AGG | + | chr3.4:96313406-96313425 | None:intergenic | 25.0% |
| !!! | TATGCTCAAAATTTTGATGA+TGG | - | chr3.4:96313412-96313431 | MS.gene69110:CDS | 25.0% |
| !!! | TTTTGATGATGGTAATTTGG+AGG | - | chr3.4:96313423-96313442 | MS.gene69110:CDS | 30.0% |
| AACAACACTTATAGAACCGT+TGG | - | chr3.4:96313364-96313383 | MS.gene69110:CDS | 35.0% | |
| ATCATACCTAAAACCACCAA+CGG | + | chr3.4:96313383-96313402 | None:intergenic | 35.0% | |
| TCTCCTATAAGAAGCAAAAC+AGG | - | chr3.4:96313277-96313296 | MS.gene69110:CDS | 35.0% | |
| AACACTTATAGAACCGTTGG+TGG | - | chr3.4:96313367-96313386 | MS.gene69110:CDS | 40.0% | |
| AATTGAAGCTTCCATGGAAG+AGG | - | chr3.4:96313335-96313354 | MS.gene69110:CDS | 40.0% | |
| ATAAGAAGCAAAACAGGTGG+AGG | - | chr3.4:96313283-96313302 | MS.gene69110:CDS | 40.0% | |
| CCTATAAGAAGCAAAACAGG+TGG | - | chr3.4:96313280-96313299 | MS.gene69110:CDS | 40.0% | |
| GTCTCAAATTGAAGCTTCCA+TGG | - | chr3.4:96313329-96313348 | MS.gene69110:CDS | 40.0% | |
| TTGCTTCTTATAGGAGACGA+TGG | + | chr3.4:96313274-96313293 | None:intergenic | 40.0% | |
| TTGTTTGTTCTCCTCTTCCA+TGG | + | chr3.4:96313349-96313368 | None:intergenic | 40.0% | |
| ! | CCACCTGTTTTGCTTCTTAT+AGG | + | chr3.4:96313283-96313302 | None:intergenic | 40.0% |
| !! | TGATGATGGTAATTTGGAGG+AGG | - | chr3.4:96313426-96313445 | MS.gene69110:CDS | 40.0% |
| !!! | ATAGAACCGTTGGTGGTTTT+AGG | - | chr3.4:96313374-96313393 | MS.gene69110:CDS | 40.0% |
| !!! | TGCTTGAGTGTTTTAGCTGA+AGG | + | chr3.4:96313499-96313518 | None:intergenic | 40.0% |
| GACGAAGAATCTTTAGGTCG+CGG | - | chr3.4:96313451-96313470 | MS.gene69110:CDS | 45.0% | |
| GAGGACGACGAAGAATCTTT+AGG | - | chr3.4:96313445-96313464 | MS.gene69110:CDS | 45.0% | |
| ! | GAGGGTTGCTTTCATGCTTT+GGG | - | chr3.4:96313305-96313324 | MS.gene69110:CDS | 45.0% |
| AGAAGCAAAACAGGTGGAGG+AGG | - | chr3.4:96313286-96313305 | MS.gene69110:CDS | 50.0% | |
| GAAGCAAAACAGGTGGAGGA+GGG | - | chr3.4:96313287-96313306 | MS.gene69110:CDS | 50.0% | |
| ! | GGAGGGTTGCTTTCATGCTT+TGG | - | chr3.4:96313304-96313323 | MS.gene69110:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 96313250 | 96313522 | 96313250 | ID=MS.gene69110 |
| chr3.4 | mRNA | 96313250 | 96313522 | 96313250 | ID=MS.gene69110.t1;Parent=MS.gene69110 |
| chr3.4 | exon | 96313250 | 96313522 | 96313250 | ID=MS.gene69110.t1.exon1;Parent=MS.gene69110.t1 |
| chr3.4 | CDS | 96313250 | 96313522 | 96313250 | ID=cds.MS.gene69110.t1;Parent=MS.gene69110.t1 |
| Gene Sequence |
| Protein sequence |
