AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene78314


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78314.t1 MTR_7g114210 97.143 175 5 0 1 175 1 175 5.13e-128 357
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene78314.t1 AT3G05000 79.429 175 33 2 1 175 1 172 2.63e-101 289

Find 46 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCGGTTTGGTGATCATT+TGG 0.323253 7.4:-2945733 MS.gene78314:CDS
GATCGAAGCCATTGGTTATC+AGG 0.347972 7.4:-2946123 MS.gene78314:CDS
ATCCTGCGAGCAGCGAGTTC+AGG 0.362814 7.4:+2946143 None:intergenic
TTCCCATGCGGGATCATTAG+AGG 0.368149 7.4:-2945371 MS.gene78314:CDS
GATATACGATGGAGCGGCCT+CGG 0.415640 7.4:-2945749 MS.gene78314:CDS
TAGAGGAGCTCTTTCCAACT+TGG 0.425869 7.4:-2945354 MS.gene78314:CDS
TGCACCTACGGCTGAAAGTA+AGG 0.438829 7.4:-2945423 MS.gene78314:CDS
CTAGAGTAGTTCATACTCAA+TGG 0.454113 7.4:-2945091 MS.gene78314:CDS
GGCAATTAAGTTCATTTGCA+AGG 0.458740 7.4:-2945709 MS.gene78314:CDS
AGCAGCGAGTTCAGGTTTGT+TGG 0.468848 7.4:+2946151 None:intergenic
GGCTGAAAGTAAGGCAGCAC+AGG 0.488115 7.4:-2945414 MS.gene78314:CDS
ACGATGGAGCGGCCTCGGTT+TGG 0.496970 7.4:-2945744 MS.gene78314:CDS
ATAAATTTCCGTGGCTTGCA+CGG 0.497008 7.4:-2945496 MS.gene78314:CDS
AGAGGAGCTCTTTCCAACTT+GGG 0.498177 7.4:-2945353 MS.gene78314:CDS
TGCATCTTTATTTCCCATGC+GGG 0.509752 7.4:-2945382 MS.gene78314:CDS
TAGAGTAGTTCATACTCAAT+GGG 0.517531 7.4:-2945090 MS.gene78314:CDS
TCCGTGGCTTGCACGGATGT+CGG 0.520572 7.4:-2945489 MS.gene78314:CDS
TCGGTTTGGTGATCATTTGG+AGG 0.520959 7.4:-2945730 MS.gene78314:CDS
ACAACTTGAAAACTAACCAT+AGG 0.528003 7.4:-2945653 MS.gene78314:CDS
AACCTGAACTCGCTGCTCGC+AGG 0.543806 7.4:-2946145 MS.gene78314:CDS
CAACTTGAAAACTAACCATA+GGG 0.547452 7.4:-2945652 MS.gene78314:intron
TGATAATGTTAGTTCAGTTG+AGG 0.553497 7.4:-2945453 MS.gene78314:CDS
TGCACAGGGAATTCCCAAGT+TGG 0.556321 7.4:+2945340 None:intergenic
TGAGGATTATTCTGCACCTA+CGG 0.558019 7.4:-2945435 MS.gene78314:CDS
GCTGCCTTACTTTCAGCCGT+AGG 0.558260 7.4:+2945419 None:intergenic
ACCGACATCCGTGCAAGCCA+CGG 0.566327 7.4:+2945488 None:intergenic
TATGTCTGCAGAAACTGCAC+AGG 0.568585 7.4:+2945325 None:intergenic
TTCAGAGAGTGAAAATGGTG+AGG 0.569906 7.4:-2946244 None:intergenic
GAACTAACATTATCAGTGGA+TGG 0.571137 7.4:+2945461 None:intergenic
TTGCGTTGATAGCTTGATGA+CGG 0.587460 7.4:-2946207 MS.gene78314:CDS
GCTCCTCTAATGATCCCGCA+TGG 0.591236 7.4:+2945368 None:intergenic
CTCCTCTAATGATCCCGCAT+GGG 0.596153 7.4:+2945369 None:intergenic
TGCAAGATAATAAATTTCCG+TGG 0.603647 7.4:-2945505 MS.gene78314:CDS
TCAGAGAGTGAAAATGGTGA+GGG 0.605160 7.4:-2946243 None:intergenic
TCGGTCAACAGCTCTCTGAA+AGG 0.608978 7.4:-2946100 MS.gene78314:intron
GCTCGCAGGATCGAAGCCAT+TGG 0.613190 7.4:-2946131 MS.gene78314:CDS
GGGGATACACACATGCTGGA+AGG 0.619761 7.4:+2945297 None:intergenic
GAAGCCATTGGTTATCAGGT+CGG 0.622295 7.4:-2946119 MS.gene78314:CDS
AACTGAACTAACATTATCAG+TGG 0.624516 7.4:+2945457 None:intergenic
TTGACCGACCTGATAACCAA+TGG 0.626958 7.4:+2946115 None:intergenic
TGTGTAATTCAGATATACGA+TGG 0.627817 7.4:-2945760 MS.gene78314:intron
TGATAGCTTGATGACGGAGA+TGG 0.641800 7.4:-2946201 MS.gene78314:CDS
ATGCATCTTTATTTCCCATG+CGG 0.650443 7.4:-2945383 MS.gene78314:CDS
AATTCAGATATACGATGGAG+CGG 0.678919 7.4:-2945755 MS.gene78314:intron
TCCAAATGATCACCAAACCG+AGG 0.695841 7.4:+2945732 None:intergenic
ATGTCTGCAGAAACTGCACA+GGG 0.724210 7.4:+2945326 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATATAT+TGG - chr7.4:2945686-2945705 MS.gene78314:CDS 0.0%
!!! TTCTTGATATTAATTATTTT+TGG - chr7.4:2945292-2945311 MS.gene78314:intron 10.0%
!!! TGATATTAATTATTTTTGGT+AGG - chr7.4:2945296-2945315 MS.gene78314:intron 15.0%
!! TCATGAAATTGTAATCAAAT+TGG - chr7.4:2945341-2945360 MS.gene78314:CDS 20.0%
!!! TTTTCATTTTTAGTGTTTTC+TGG - chr7.4:2946112-2946131 MS.gene78314:CDS 20.0%
! TGTGAATAATTTGATCTGTT+TGG - chr7.4:2946163-2946182 MS.gene78314:CDS 25.0%
! TGTTTGGAAATGTTTAAACA+GGG - chr7.4:2946179-2946198 MS.gene78314:CDS 25.0%
!! ATTTTCTCGAATTACTACAT+AGG + chr7.4:2945409-2945428 None:intergenic 25.0%
!! CATAGAAGAGAAGATTTTTA+AGG - chr7.4:2946074-2946093 MS.gene78314:intron 25.0%
!!! TTTTTGTGTTTTGATTTCCA+TGG - chr7.4:2945729-2945748 MS.gene78314:CDS 25.0%
AACTGAACTAACATTATCAG+TGG + chr7.4:2945840-2945859 None:intergenic 30.0%
ACAACTTGAAAACTAACCAT+AGG - chr7.4:2945641-2945660 MS.gene78314:intron 30.0%
AGATGAAGAAAGAGAGAATT+AGG + chr7.4:2945235-2945254 None:intergenic 30.0%
CAACTTGAAAACTAACCATA+GGG - chr7.4:2945642-2945661 MS.gene78314:intron 30.0%
CTGTTTGGAAATGTTTAAAC+AGG - chr7.4:2946178-2946197 MS.gene78314:CDS 30.0%
GATGAAGAAAGAGAGAATTA+GGG + chr7.4:2945234-2945253 None:intergenic 30.0%
TAGAGTAGTTCATACTCAAT+GGG - chr7.4:2946204-2946223 MS.gene78314:CDS 30.0%
TGCAAGATAATAAATTTCCG+TGG - chr7.4:2945789-2945808 MS.gene78314:intron 30.0%
TGTGTAATTCAGATATACGA+TGG - chr7.4:2945534-2945553 MS.gene78314:intron 30.0%
! AGTTCATTTGCAAGGATTTT+TGG - chr7.4:2945593-2945612 MS.gene78314:intron 30.0%
! ATTTCCATGGTTGTGTTATT+TGG - chr7.4:2945742-2945761 MS.gene78314:CDS 30.0%
! TGATAATGTTAGTTCAGTTG+AGG - chr7.4:2945841-2945860 MS.gene78314:intron 30.0%
AATTCAGATATACGATGGAG+CGG - chr7.4:2945539-2945558 MS.gene78314:intron 35.0%
AGAACCAAATAACACAACCA+TGG + chr7.4:2945749-2945768 None:intergenic 35.0%
CTAGAGTAGTTCATACTCAA+TGG - chr7.4:2946203-2946222 MS.gene78314:CDS 35.0%
CTGTTATCACACAACATAGT+AGG - chr7.4:2945259-2945278 MS.gene78314:intron 35.0%
GAACTAACATTATCAGTGGA+TGG + chr7.4:2945836-2945855 None:intergenic 35.0%
GGCAATTAAGTTCATTTGCA+AGG - chr7.4:2945585-2945604 MS.gene78314:intron 35.0%
! ATGCATCTTTATTTCCCATG+CGG - chr7.4:2945911-2945930 MS.gene78314:intron 35.0%
AACCAATTCAACTCACCCTA+TGG + chr7.4:2945660-2945679 None:intergenic 40.0%
GAATGTAGTCACATGTGTCA+GGG - chr7.4:2945438-2945457 MS.gene78314:CDS 40.0%
TGAATGTAGTCACATGTGTC+AGG - chr7.4:2945437-2945456 MS.gene78314:CDS 40.0%
TGAGGATTATTCTGCACCTA+CGG - chr7.4:2945859-2945878 MS.gene78314:intron 40.0%
! AACCATAGGGTGAGTTGAAT+TGG - chr7.4:2945655-2945674 MS.gene78314:CDS 40.0%
! ATAAATTTCCGTGGCTTGCA+CGG - chr7.4:2945798-2945817 MS.gene78314:intron 40.0%
! GTCAGATAGGTTATGATGGA+TGG - chr7.4:2945460-2945479 MS.gene78314:CDS 40.0%
! TGCATCTTTATTTCCCATGC+GGG - chr7.4:2945912-2945931 MS.gene78314:intron 40.0%
!! TGTTATTTGGTTCTGCTGCA+GGG - chr7.4:2945755-2945774 MS.gene78314:intron 40.0%
!! TTGCGTTGATAGCTTGATGA+CGG - chr7.4:2945087-2945106 MS.gene78314:CDS 40.0%
!!! TTGCAAGGATTTTTGGTCTG+AGG - chr7.4:2945600-2945619 MS.gene78314:intron 40.0%
ACTACATAGGTCGTGCTTCA+TGG + chr7.4:2945396-2945415 None:intergenic 45.0%
AGAGGAGCTCTTTCCAACTT+GGG - chr7.4:2945941-2945960 MS.gene78314:intron 45.0%
ATGTCTGCAGAAACTGCACA+GGG + chr7.4:2945971-2945990 None:intergenic 45.0%
CAGGGTCAGATAGGTTATGA+TGG - chr7.4:2945456-2945475 MS.gene78314:CDS 45.0%
TAGAGGAGCTCTTTCCAACT+TGG - chr7.4:2945940-2945959 MS.gene78314:intron 45.0%
TATGTCTGCAGAAACTGCAC+AGG + chr7.4:2945972-2945991 None:intergenic 45.0%
TCCAAATGATCACCAAACCG+AGG + chr7.4:2945565-2945584 None:intergenic 45.0%
TTGACCGACCTGATAACCAA+TGG + chr7.4:2945182-2945201 None:intergenic 45.0%
! GAAGCCATTGGTTATCAGGT+CGG - chr7.4:2945175-2945194 MS.gene78314:intron 45.0%
! GATCGAAGCCATTGGTTATC+AGG - chr7.4:2945171-2945190 MS.gene78314:intron 45.0%
! TCAACAGCTCTCTGAAAGGT+TGG - chr7.4:2945198-2945217 MS.gene78314:intron 45.0%
!! GTGTTATTTGGTTCTGCTGC+AGG - chr7.4:2945754-2945773 MS.gene78314:intron 45.0%
!! TCGGTTTGGTGATCATTTGG+AGG - chr7.4:2945564-2945583 MS.gene78314:intron 45.0%
!! TGATAGCTTGATGACGGAGA+TGG - chr7.4:2945093-2945112 MS.gene78314:CDS 45.0%
AGAGAGAGAGAGAGAGAGAG+AGG + chr7.4:2946028-2946047 None:intergenic 50.0%
AGAGAGAGAGAGAGGGAGAT+GGG + chr7.4:2946022-2946041 None:intergenic 50.0%
CACATGTGTCAGGGTCAGAT+AGG - chr7.4:2945447-2945466 MS.gene78314:CDS 50.0%
CTCCTCTAATGATCCCGCAT+GGG + chr7.4:2945928-2945947 None:intergenic 50.0%
GAGAGAGAGAGAGAGAGAGA+GGG + chr7.4:2946029-2946048 None:intergenic 50.0%
TCGGTCAACAGCTCTCTGAA+AGG - chr7.4:2945194-2945213 MS.gene78314:intron 50.0%
TGCACAGGGAATTCCCAAGT+TGG + chr7.4:2945957-2945976 None:intergenic 50.0%
TGCACCTACGGCTGAAAGTA+AGG - chr7.4:2945871-2945890 MS.gene78314:intron 50.0%
TTCCCATGCGGGATCATTAG+AGG - chr7.4:2945923-2945942 MS.gene78314:intron 50.0%
! AGCAGCGAGTTCAGGTTTGT+TGG + chr7.4:2945146-2945165 None:intergenic 50.0%
!! GCCTCGGTTTGGTGATCATT+TGG - chr7.4:2945561-2945580 MS.gene78314:intron 50.0%
AGAGAGAGAGAGGGAGATGG+GGG + chr7.4:2946020-2946039 None:intergenic 55.0%
ATGGGGGGATACACACATGC+TGG + chr7.4:2946004-2946023 None:intergenic 55.0%
GAGAGAGAGAGAGAGGGAGA+TGG + chr7.4:2946023-2946042 None:intergenic 55.0%
GAGAGAGAGAGAGGGAGATG+GGG + chr7.4:2946021-2946040 None:intergenic 55.0%
GATATACGATGGAGCGGCCT+CGG - chr7.4:2945545-2945564 MS.gene78314:intron 55.0%
GCTCCTCTAATGATCCCGCA+TGG + chr7.4:2945929-2945948 None:intergenic 55.0%
GCTGCCTTACTTTCAGCCGT+AGG + chr7.4:2945878-2945897 None:intergenic 55.0%
GGCTGAAAGTAAGGCAGCAC+AGG - chr7.4:2945880-2945899 MS.gene78314:intron 55.0%
GGGGATACACACATGCTGGA+AGG + chr7.4:2946000-2946019 None:intergenic 55.0%
AACCTGAACTCGCTGCTCGC+AGG - chr7.4:2945149-2945168 MS.gene78314:intron 60.0%
ACCGACATCCGTGCAAGCCA+CGG + chr7.4:2945809-2945828 None:intergenic 60.0%
ATCCTGCGAGCAGCGAGTTC+AGG + chr7.4:2945154-2945173 None:intergenic 60.0%
GAGAGAGAGAGGGAGATGGG+GGG + chr7.4:2946019-2946038 None:intergenic 60.0%
GCTCGCAGGATCGAAGCCAT+TGG - chr7.4:2945163-2945182 MS.gene78314:intron 60.0%
! TCCGTGGCTTGCACGGATGT+CGG - chr7.4:2945805-2945824 MS.gene78314:intron 60.0%
ACGATGGAGCGGCCTCGGTT+TGG - chr7.4:2945550-2945569 MS.gene78314:intron 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 2945064 2946252 2945064 ID=MS.gene78314
chr7.4 mRNA 2945064 2946252 2945064 ID=MS.gene78314.t1;Parent=MS.gene78314
chr7.4 exon 2946101 2946252 2946101 ID=MS.gene78314.t1.exon1;Parent=MS.gene78314.t1
chr7.4 CDS 2946101 2946252 2946101 ID=cds.MS.gene78314.t1;Parent=MS.gene78314.t1
chr7.4 exon 2945653 2945770 2945653 ID=MS.gene78314.t1.exon2;Parent=MS.gene78314.t1
chr7.4 CDS 2945653 2945770 2945653 ID=cds.MS.gene78314.t1;Parent=MS.gene78314.t1
chr7.4 exon 2945309 2945540 2945309 ID=MS.gene78314.t1.exon3;Parent=MS.gene78314.t1
chr7.4 CDS 2945309 2945540 2945309 ID=cds.MS.gene78314.t1;Parent=MS.gene78314.t1
chr7.4 exon 2945064 2945116 2945064 ID=MS.gene78314.t1.exon4;Parent=MS.gene78314.t1
chr7.4 CDS 2945064 2945116 2945064 ID=cds.MS.gene78314.t1;Parent=MS.gene78314.t1
Gene Sequence

>MS.gene78314

ATGGTGAGGGAAGTTTCAGAGAGTTGCGTTGATAGCTTGATGACGGAGATGGTTGCTTGTTATTGCAATCGATTCTATGCCAACAAACCTGAACTCGCTGCTCGCAGGATCGAAGCCATTGGTTATCAGGTCGGTCAACAGCTCTCTGAAAGGTTGGTTGTAACAAACCCTAATTCTCTCTTTCTTCATCTAGATCTGTTATCACACAACATAGTAGGATTATTCTATTTCTTGATATTAATTATTTTTGGTAGGTTGATTCTGTATTGCTTTTGTATCATGAAATTGTAATCAAATTGGTTAGACTATGATTGATTAGTTGTTATTATCCATGAAGCACGACCTATGTAGTAATTCGAGAAAATGAAATAATTGAATGTAGTCACATGTGTCAGGGTCAGATAGGTTATGATGGATGGTTCACTGAAGTAAGTTATGATTAAATTGCAATGAATTGAATAAATGTGATGTGTGTAATTCAGATATACGATGGAGCGGCCTCGGTTTGGTGATCATTTGGAGGCAATTAAGTTCATTTGCAAGGATTTTTGGTCTGAGGTGTTTAAGAAGCAGATAGACAACTTGAAAACTAACCATAGGGTGAGTTGAATTGGTTGAATGTATATATATATATATATATATATATATATATATATATATTGGTATTTTTGTGTTTTGATTTCCATGGTTGTGTTATTTGGTTCTGCTGCAGGGTACATTTGTGTTGCAAGATAATAAATTTCCGTGGCTTGCACGGATGTCGGTTGATCCATCCACTGATAATGTTAGTTCAGTTGAGGATTATTCTGCACCTACGGCTGAAAGTAAGGCAGCACAGGCAATGAGCATGCATCTTTATTTCCCATGCGGGATCATTAGAGGAGCTCTTTCCAACTTGGGAATTCCCTGTGCAGTTTCTGCAGACATATCAAGCCTTCCAGCATGTGTGTATCCCCCCATCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACATAGAAGAGAAGATTTTTAAGGATAGCAAAGAGTTTCTTTTCATTTTTAGTGTTTTCTGGATGCACAATTTGCATGTGACAAGTCTGATGTGAATAATTTGATCTGTTTGGAAATGTTTAAACAGGGTCTAGAGTAGTTCATACTCAATGGGTTACAGTGAGAAGTTCAAGAGTGTGA

Protein sequence

>MS.gene78314.t1

MVREVSESCVDSLMTEMVACYCNRFYANKPELAARRIEAIGYQVGQQLSERYTMERPRFGDHLEAIKFICKDFWSEVFKKQIDNLKTNHRGTFVLQDNKFPWLARMSVDPSTDNVSSVEDYSAPTAESKAAQAMSMHLYFPCGIIRGALSNLGIPCAVSADISSLPAWSRVVHTQWVTVRSSRV