AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene80235


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80235.t1 MTR_3g106750 95.420 131 3 2 1 130 1 129 2.33e-63 189
MS.gene80235.t1 MTR_3g106740 74.615 130 31 2 1 129 1 129 2.05e-56 172
MS.gene80235.t1 MTR_1g012690 57.325 157 37 4 1 130 1 154 1.94e-50 157
MS.gene80235.t1 MTR_1g012700 56.627 166 34 4 1 130 1 164 2.16e-49 155
MS.gene80235.t1 MTR_1g012710 60.784 153 35 3 1 130 1 151 1.01e-44 143
MS.gene80235.t1 MTR_1g012630 79.762 84 16 1 47 130 128 210 1.74e-41 137
MS.gene80235.t1 MTR_4g108150 50.820 122 56 2 9 130 6 123 6.67e-33 112
MS.gene80235.t1 MTR_4g101260 63.095 84 30 1 47 130 50 132 4.88e-31 107
MS.gene80235.t1 MTR_4g101330 40.588 170 58 6 1 129 1 168 2.60e-29 104
MS.gene80235.t1 MTR_4g101280 56.818 88 36 2 44 130 57 143 9.70e-29 102
MS.gene80235.t1 MTR_2g026760 47.619 126 61 3 4 129 6 126 1.38e-28 101
MS.gene80235.t1 MTR_4g101310 57.647 85 34 2 47 130 51 134 8.93e-28 99.8
MS.gene80235.t1 MTR_4g101270 57.143 84 33 3 47 129 105 186 1.58e-22 87.8
MS.gene80235.t1 MTR_5g012340 47.619 84 41 2 47 129 95 176 1.70e-22 87.4
MS.gene80235.t1 MTR_5g012380 46.429 84 42 2 47 129 88 169 7.90e-22 85.5
MS.gene80235.t1 MTR_2g026930 48.750 80 38 2 50 129 122 198 1.57e-16 72.4
MS.gene80235.t1 MTR_2g026920 48.750 80 38 2 50 129 118 194 1.70e-16 72.4
MS.gene80235.t1 MTR_3g082100 46.512 86 45 1 44 129 251 335 1.26e-15 72.0
MS.gene80235.t1 MTR_2g026775 41.772 79 40 3 54 130 686 760 6.78e-13 64.7
MS.gene80235.t1 MTR_4g115360 48.235 85 41 3 47 130 151 233 2.63e-11 58.9
MS.gene80235.t1 MTR_2g026820 52.778 72 27 4 52 121 542 608 3.67e-11 59.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene80235.t1 AT2G45180 69.048 84 25 1 47 130 52 134 4.20e-34 115
MS.gene80235.t1 AT4G12490 60.000 95 36 2 37 130 89 182 5.62e-32 112
MS.gene80235.t1 AT4G12500 38.764 178 60 4 1 130 1 177 1.85e-31 110
MS.gene80235.t1 AT4G12480 60.920 87 32 2 45 130 83 168 5.27e-31 108
MS.gene80235.t1 AT4G12470 58.889 90 35 2 42 130 73 161 8.28e-31 108
MS.gene80235.t1 AT5G46900 63.736 91 29 2 40 130 41 127 3.19e-30 105
MS.gene80235.t1 AT1G62510 61.176 85 31 2 46 129 66 149 1.55e-29 104
MS.gene80235.t1 AT5G46890 61.053 95 33 3 36 130 37 127 1.19e-28 101
MS.gene80235.t1 AT1G12090 62.195 82 29 2 49 129 57 137 1.30e-28 102
MS.gene80235.t1 AT4G12520 52.893 121 53 3 11 130 12 129 4.78e-28 100
MS.gene80235.t1 AT4G12510 52.893 121 53 3 11 130 12 129 4.78e-28 100
MS.gene80235.t1 AT4G12550 50.000 92 44 2 40 130 21 111 4.52e-26 94.7
MS.gene80235.t1 AT4G00165 47.664 107 51 3 27 129 23 128 5.45e-26 95.1
MS.gene80235.t1 AT4G00165 47.664 107 51 3 27 129 23 128 5.45e-26 95.1
MS.gene80235.t1 AT1G12100 41.129 124 70 3 9 130 10 132 8.98e-26 94.7
MS.gene80235.t1 AT4G22460 48.571 105 49 2 28 128 28 131 2.15e-25 93.6
MS.gene80235.t1 AT4G12530 50.000 86 42 1 44 129 31 115 5.32e-23 87.0
MS.gene80235.t1 AT4G12545 50.000 92 41 3 40 130 21 108 6.03e-23 86.7
MS.gene80235.t1 AT3G22142 52.381 84 37 3 47 129 1397 1478 2.04e-20 86.3
MS.gene80235.t1 AT3G22120 51.190 84 39 2 47 129 267 349 5.72e-19 81.3
MS.gene80235.t1 AT3G22120 51.190 84 39 2 47 129 250 332 1.18e-18 80.1
MS.gene80235.t1 AT2G10940 47.727 88 43 3 44 130 205 290 1.24e-18 79.7
MS.gene80235.t1 AT2G10940 47.727 88 43 3 44 130 205 290 1.24e-18 79.7
MS.gene80235.t1 AT4G15160 49.412 85 40 3 47 129 99 182 4.34e-17 73.9
MS.gene80235.t1 AT4G15160 49.412 85 40 3 47 129 181 264 1.11e-16 74.3
MS.gene80235.t1 AT1G62500 42.529 87 47 3 44 129 209 293 6.54e-15 69.7
MS.gene80235.t1 AT4G22490 37.634 93 54 3 38 129 27 116 8.04e-15 66.2

Find 44 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCCTAACTTTAAGGTATCTT+TGG 0.166346 3.4:-93008425 None:intergenic
AGCACAAACCCCTAACTTTA+AGG 0.205248 3.4:-93008434 None:intergenic
ATCTTTGGGGCAGTGACCTT+GGG 0.214560 3.4:-93008410 None:intergenic
TTGTCTTTGCACTGCAATTA+AGG 0.231170 3.4:+93008555 MS.gene80235:CDS
AATCAAAGGTTTGGCTGATT+TGG 0.237754 3.4:+93008522 MS.gene80235:CDS
TAAAATGGTGGCAAAGAATT+TGG 0.290687 3.4:-93008296 None:intergenic
CCTAACTTTAAGGTATCTTT+GGG 0.293316 3.4:-93008424 None:intergenic
GAAGGTGTTGGCTTTGGCTT+TGG 0.296299 3.4:-93008385 None:intergenic
TGGGGTGAAGGTGTTGGCTT+TGG 0.321997 3.4:-93008391 None:intergenic
CCCAAAGATACCTTAAAGTT+AGG 0.331524 3.4:+93008424 MS.gene80235:CDS
GTTGGCTTTGGCTTTGGCTT+TGG 0.335137 3.4:-93008379 None:intergenic
TATCTTTGGGGCAGTGACCT+TGG 0.380498 3.4:-93008411 None:intergenic
CCAAAGATACCTTAAAGTTA+GGG 0.385359 3.4:+93008425 MS.gene80235:CDS
AGGATCCAGGTGAGGGTGAT+AGG 0.388310 3.4:-93008607 None:intergenic
GGGCAGTGACCTTGGGGTGA+AGG 0.389809 3.4:-93008403 None:intergenic
AAGGAGAGAGAGTACTAAAA+TGG 0.390388 3.4:-93008311 None:intergenic
ATCACCCTCACCTGGATCCT+TGG 0.406696 3.4:+93008610 MS.gene80235:CDS
GTTGTTGGGAATCCTGCTTC+AGG 0.413980 3.4:+93008481 MS.gene80235:CDS
TTTGGCTTTGGCTTTGGTGA+TGG 0.416452 3.4:-93008373 None:intergenic
CTCAACATTTACTGAGGCTA+AGG 0.420857 3.4:+93008339 MS.gene80235:CDS
TTGACAAGCACCAAGGATCC+AGG 0.457767 3.4:-93008620 None:intergenic
GACTGGTTAATGTTGTTGTT+GGG 0.463209 3.4:+93008467 MS.gene80235:CDS
TTGTGCAATAATCAAAGGTT+TGG 0.466120 3.4:+93008513 MS.gene80235:CDS
GGACTGGTTAATGTTGTTGT+TGG 0.480624 3.4:+93008466 MS.gene80235:CDS
TGACCTTGGGGTGAAGGTGT+TGG 0.502306 3.4:-93008397 None:intergenic
AATTGTTGTGCAATAATCAA+AGG 0.523157 3.4:+93008508 MS.gene80235:CDS
GCAATTAAGGCCAATGTGCT+CGG 0.533371 3.4:+93008568 MS.gene80235:CDS
TCTTTGGGGCAGTGACCTTG+GGG 0.545751 3.4:-93008409 None:intergenic
CCTTAGGCACATTGAAAACC+AGG 0.555069 3.4:-93008655 None:intergenic
CAAAGATACCTTAAAGTTAG+GGG 0.560461 3.4:+93008426 MS.gene80235:CDS
ATGTACCTATCACCCTCACC+TGG 0.591606 3.4:+93008602 MS.gene80235:CDS
CAGTAAATGTTGAGTAAGCA+AGG 0.592270 3.4:-93008330 None:intergenic
TCAACATTTACTGAGGCTAA+GGG 0.596026 3.4:+93008340 MS.gene80235:CDS
CAAGTTTATTCCGAGCACAT+TGG 0.596849 3.4:-93008578 None:intergenic
GGTGGCAAAGAATTTGGTGT+TGG 0.599992 3.4:-93008290 None:intergenic
CAACAATTACTACCTGAAGC+AGG 0.602543 3.4:-93008493 None:intergenic
AGCACCAAGGATCCAGGTGA+GGG 0.612333 3.4:-93008614 None:intergenic
AAGCCAACACCTTCACCCCA+AGG 0.614009 3.4:+93008394 MS.gene80235:CDS
TGCTTACTCAACATTTACTG+AGG 0.622636 3.4:+93008333 MS.gene80235:CDS
GAGAGAGAGTACTAAAATGG+TGG 0.630559 3.4:-93008308 None:intergenic
AAGCACCAAGGATCCAGGTG+AGG 0.645068 3.4:-93008615 None:intergenic
AGGCACATTGAAAACCAGGA+GGG 0.674973 3.4:-93008651 None:intergenic
CTAACTTTAAGGTATCTTTG+GGG 0.708820 3.4:-93008423 None:intergenic
TAGGCACATTGAAAACCAGG+AGG 0.727521 3.4:-93008652 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AATTGTTGTGCAATAATCAA+AGG + chr3.4:93008508-93008527 MS.gene80235:CDS 25.0%
CAAAGATACCTTAAAGTTAG+GGG + chr3.4:93008426-93008445 MS.gene80235:CDS 30.0%
CCAAAGATACCTTAAAGTTA+GGG + chr3.4:93008425-93008444 MS.gene80235:CDS 30.0%
TAAAATGGTGGCAAAGAATT+TGG - chr3.4:93008299-93008318 None:intergenic 30.0%
TTGTGCAATAATCAAAGGTT+TGG + chr3.4:93008513-93008532 MS.gene80235:CDS 30.0%
!! CCTAACTTTAAGGTATCTTT+GGG - chr3.4:93008427-93008446 None:intergenic 30.0%
!! CTAACTTTAAGGTATCTTTG+GGG - chr3.4:93008426-93008445 None:intergenic 30.0%
AATCAAAGGTTTGGCTGATT+TGG + chr3.4:93008522-93008541 MS.gene80235:CDS 35.0%
CAGTAAATGTTGAGTAAGCA+AGG - chr3.4:93008333-93008352 None:intergenic 35.0%
CCCAAAGATACCTTAAAGTT+AGG + chr3.4:93008424-93008443 MS.gene80235:CDS 35.0%
TCAACATTTACTGAGGCTAA+GGG + chr3.4:93008340-93008359 MS.gene80235:CDS 35.0%
TGCTTACTCAACATTTACTG+AGG + chr3.4:93008333-93008352 MS.gene80235:CDS 35.0%
TTGTCTTTGCACTGCAATTA+AGG + chr3.4:93008555-93008574 MS.gene80235:CDS 35.0%
! GACTGGTTAATGTTGTTGTT+GGG + chr3.4:93008467-93008486 MS.gene80235:CDS 35.0%
!! AAGGAGAGAGAGTACTAAAA+TGG - chr3.4:93008314-93008333 None:intergenic 35.0%
!! CCCTAACTTTAAGGTATCTT+TGG - chr3.4:93008428-93008447 None:intergenic 35.0%
!!! TTGTTTTTTGACAAGCACCA+AGG - chr3.4:93008630-93008649 None:intergenic 35.0%
AGCACAAACCCCTAACTTTA+AGG - chr3.4:93008437-93008456 None:intergenic 40.0%
CAACAATTACTACCTGAAGC+AGG - chr3.4:93008496-93008515 None:intergenic 40.0%
CAAGTTTATTCCGAGCACAT+TGG - chr3.4:93008581-93008600 None:intergenic 40.0%
CTCAACATTTACTGAGGCTA+AGG + chr3.4:93008339-93008358 MS.gene80235:CDS 40.0%
TGTCAAAAAACAATCCCTCC+TGG + chr3.4:93008637-93008656 MS.gene80235:CDS 40.0%
!! GAGAGAGAGTACTAAAATGG+TGG - chr3.4:93008311-93008330 None:intergenic 40.0%
!! GGACTGGTTAATGTTGTTGT+TGG + chr3.4:93008466-93008485 MS.gene80235:CDS 40.0%
!!! TTGTGCTGATGTTTTAGGAC+TGG + chr3.4:93008450-93008469 MS.gene80235:CDS 40.0%
AGGCACATTGAAAACCAGGA+GGG - chr3.4:93008654-93008673 None:intergenic 45.0%
GCAATTAAGGCCAATGTGCT+CGG + chr3.4:93008568-93008587 MS.gene80235:CDS 45.0%
TAGGCACATTGAAAACCAGG+AGG - chr3.4:93008655-93008674 None:intergenic 45.0%
!! GGGGTTTGTGCTGATGTTTT+AGG + chr3.4:93008445-93008464 MS.gene80235:CDS 45.0%
!! GGTGGCAAAGAATTTGGTGT+TGG - chr3.4:93008293-93008312 None:intergenic 45.0%
!! TTTGGCTTTGGCTTTGGTGA+TGG - chr3.4:93008376-93008395 None:intergenic 45.0%
ATGTACCTATCACCCTCACC+TGG + chr3.4:93008602-93008621 MS.gene80235:CDS 50.0%
GTTGTTGGGAATCCTGCTTC+AGG + chr3.4:93008481-93008500 MS.gene80235:CDS 50.0%
TTGACAAGCACCAAGGATCC+AGG - chr3.4:93008623-93008642 None:intergenic 50.0%
!! ATCTTTGGGGCAGTGACCTT+GGG - chr3.4:93008413-93008432 None:intergenic 50.0%
!! GAAGGTGTTGGCTTTGGCTT+TGG - chr3.4:93008388-93008407 None:intergenic 50.0%
!! GTTGGCTTTGGCTTTGGCTT+TGG - chr3.4:93008382-93008401 None:intergenic 50.0%
!! TATCTTTGGGGCAGTGACCT+TGG - chr3.4:93008414-93008433 None:intergenic 50.0%
AAGCACCAAGGATCCAGGTG+AGG - chr3.4:93008618-93008637 None:intergenic 55.0%
AAGCCAACACCTTCACCCCA+AGG + chr3.4:93008394-93008413 MS.gene80235:CDS 55.0%
AGCACCAAGGATCCAGGTGA+GGG - chr3.4:93008617-93008636 None:intergenic 55.0%
AGGATCCAGGTGAGGGTGAT+AGG - chr3.4:93008610-93008629 None:intergenic 55.0%
ATCACCCTCACCTGGATCCT+TGG + chr3.4:93008610-93008629 MS.gene80235:CDS 55.0%
! TGACCTTGGGGTGAAGGTGT+TGG - chr3.4:93008400-93008419 None:intergenic 55.0%
!! TCTTTGGGGCAGTGACCTTG+GGG - chr3.4:93008412-93008431 None:intergenic 55.0%
!! TGGGGTGAAGGTGTTGGCTT+TGG - chr3.4:93008394-93008413 None:intergenic 55.0%
GGGCAGTGACCTTGGGGTGA+AGG - chr3.4:93008406-93008425 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 93008283 93008675 93008283 ID=MS.gene80235
chr3.4 mRNA 93008283 93008675 93008283 ID=MS.gene80235.t1;Parent=MS.gene80235
chr3.4 exon 93008283 93008675 93008283 ID=MS.gene80235.t1.exon1;Parent=MS.gene80235.t1
chr3.4 CDS 93008283 93008675 93008283 ID=cds.MS.gene80235.t1;Parent=MS.gene80235.t1
Gene Sequence

>MS.gene80235

ATGGCTTCCAACACCAAATTCTTTGCCACCATTTTAGTACTCTCTCTCCTTGCTTACTCAACATTTACTGAGGCTAAGGGTTCATGCTCACCATCACCAAAGCCAAAGCCAAAGCCAACACCTTCACCCCAAGGTCACTGCCCCAAAGATACCTTAAAGTTAGGGGTTTGTGCTGATGTTTTAGGACTGGTTAATGTTGTTGTTGGGAATCCTGCTTCAGGTAGTAATTGTTGTGCAATAATCAAAGGTTTGGCTGATTTGGATGCTGCACTTTGTCTTTGCACTGCAATTAAGGCCAATGTGCTCGGAATAAACTTGAATGTACCTATCACCCTCACCTGGATCCTTGGTGCTTGTCAAAAAACAATCCCTCCTGGTTTTCAATGTGCCTAA

Protein sequence

>MS.gene80235.t1

MASNTKFFATILVLSLLAYSTFTEAKGSCSPSPKPKPKPTPSPQGHCPKDTLKLGVCADVLGLVNVVVGNPASGSNCCAIIKGLADLDAALCLCTAIKANVLGINLNVPITLTWILGACQKTIPPGFQCA