AlfalfaGEDB | MS.gene83217

M. sativa cultivar XinJiangDaYe / MS.gene83217

KEGG: K02872
GO: GO:0003674 GO:0003735 GO:0005198 GO:0005575 GO:0005840 GO:0006412 GO:0006518 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0019538 GO:0034641 GO:0034645 GO:0043043 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043603 GO:0043604 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044267 GO:0044271 GO:0071704 GO:0110165 GO:1901564 GO:1901566 GO:1901576
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83217.t1	XP_013448778.1	99	100	1	0	1	100	107	206	6.20E-47	196.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83217.t1	Q9FKC0	77.0	100	23	0	1	100	107	206	1.2e-38	160.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83217.t1	B7FGF9	99.0	100	1	0	1	100	107	206	4.4e-47	196.4

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049166	MS.gene83217	0.800202	1.61E-48	-1.69E-46
MS.gene049580	MS.gene83217	0.806752	7.06E-50	-1.69E-46
MS.gene049887	MS.gene83217	0.809952	1.47E-50	-1.69E-46
MS.gene050114	MS.gene83217	0.806353	8.57E-50	-1.69E-46
MS.gene050277	MS.gene83217	0.860172	2.49E-63	-1.69E-46
MS.gene050317	MS.gene83217	0.80883	2.55E-50	-1.69E-46
MS.gene050320	MS.gene83217	0.812311	4.52E-51	-1.69E-46
MS.gene050752	MS.gene83217	0.809914	1.50E-50	-1.69E-46
MS.gene050838	MS.gene83217	0.867574	1.24E-65	-1.69E-46
MS.gene050916	MS.gene83217	0.810336	1.21E-50	-1.69E-46
MS.gene051063	MS.gene83217	0.864529	1.14E-64	-1.69E-46
MS.gene051245	MS.gene83217	0.81619	6.28E-52	-1.69E-46
MS.gene051330	MS.gene83217	0.842193	3.00E-58	-1.69E-46
MS.gene05134	MS.gene83217	0.80794	3.95E-50	-1.69E-46
MS.gene051451	MS.gene83217	0.833252	5.93E-56	-1.69E-46
MS.gene051633	MS.gene83217	0.803979	2.69E-49	-1.69E-46
MS.gene051746	MS.gene83217	0.891175	5.05E-74	-1.69E-46
MS.gene051748	MS.gene83217	0.881895	1.64E-70	-1.69E-46
MS.gene051756	MS.gene83217	0.854078	1.56E-61	-1.69E-46
MS.gene051757	MS.gene83217	0.818541	1.86E-52	-1.69E-46
MS.gene051850	MS.gene83217	0.847349	1.22E-59	-1.69E-46
MS.gene05188	MS.gene83217	0.835843	1.32E-56	-1.69E-46
MS.gene051903	MS.gene83217	0.903714	2.61E-79	-1.69E-46
MS.gene051975	MS.gene83217	0.811221	7.80E-51	-1.69E-46
MS.gene052202	MS.gene83217	0.814228	1.71E-51	-1.69E-46
MS.gene052947	MS.gene83217	0.881054	3.30E-70	-1.69E-46
MS.gene053109	MS.gene83217	0.849533	3.04E-60	-1.69E-46
MS.gene053381	MS.gene83217	0.844441	7.55E-59	-1.69E-46
MS.gene053952	MS.gene83217	0.893161	8.13E-75	-1.69E-46
MS.gene054403	MS.gene83217	0.806899	6.57E-50	-1.69E-46
MS.gene05469	MS.gene83217	0.808028	3.79E-50	-1.69E-46
MS.gene054937	MS.gene83217	0.808398	3.16E-50	-1.69E-46
MS.gene055424	MS.gene83217	0.830458	2.90E-55	-1.69E-46
MS.gene055591	MS.gene83217	-0.80588	1.08E-49	-1.69E-46
MS.gene055603	MS.gene83217	0.821819	3.30E-53	-1.69E-46
MS.gene055780	MS.gene83217	0.82264	2.13E-53	-1.69E-46
MS.gene056043	MS.gene83217	-0.801764	7.72E-49	-1.69E-46
MS.gene056075	MS.gene83217	0.840548	8.14E-58	-1.69E-46
MS.gene056432	MS.gene83217	0.835989	1.22E-56	-1.69E-46
MS.gene056580	MS.gene83217	0.802185	6.32E-49	-1.69E-46
MS.gene056601	MS.gene83217	0.808533	2.96E-50	-1.69E-46
MS.gene056631	MS.gene83217	0.866303	3.15E-65	-1.69E-46
MS.gene056986	MS.gene83217	0.820734	5.87E-53	-1.69E-46
MS.gene057057	MS.gene83217	0.84202	3.34E-58	-1.69E-46
MS.gene057094	MS.gene83217	0.914648	1.50E-84	-1.69E-46
MS.gene057140	MS.gene83217	0.817458	3.26E-52	-1.69E-46
MS.gene057234	MS.gene83217	0.869943	2.12E-66	-1.69E-46
MS.gene057705	MS.gene83217	0.800107	1.68E-48	-1.69E-46
MS.gene058055	MS.gene83217	0.823689	1.21E-53	-1.69E-46
MS.gene05809	MS.gene83217	0.815649	8.30E-52	-1.69E-46
MS.gene058238	MS.gene83217	0.840625	7.77E-58	-1.69E-46
MS.gene058654	MS.gene83217	0.808403	3.15E-50	-1.69E-46
MS.gene058906	MS.gene83217	0.877835	4.57E-69	-1.69E-46
MS.gene059171	MS.gene83217	0.810228	1.28E-50	-1.69E-46
MS.gene059227	MS.gene83217	0.813668	2.28E-51	-1.69E-46
MS.gene059726	MS.gene83217	0.819331	1.23E-52	-1.69E-46
MS.gene059830	MS.gene83217	0.808928	2.43E-50	-1.69E-46
MS.gene059898	MS.gene83217	0.873663	1.24E-67	-1.69E-46
MS.gene06001	MS.gene83217	0.913841	3.85E-84	-1.69E-46
MS.gene06011	MS.gene83217	0.878239	3.30E-69	-1.69E-46
MS.gene060463	MS.gene83217	0.826674	2.38E-54	-1.69E-46
MS.gene060465	MS.gene83217	0.830356	3.07E-55	-1.69E-46
MS.gene060586	MS.gene83217	0.813187	2.91E-51	-1.69E-46
MS.gene060763	MS.gene83217	0.853167	2.86E-61	-1.69E-46
MS.gene060830	MS.gene83217	0.808117	3.63E-50	-1.69E-46
MS.gene060917	MS.gene83217	0.806683	7.30E-50	-1.69E-46
MS.gene061145	MS.gene83217	0.861566	9.38E-64	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83217.t1	MTR_7g057770	99.000	100	1	1	100	107	206	1.19e-67	201
MS.gene83217.t1	MTR_1g023590	89.000	100	11	1	100	107	206	1.77e-59	181
MS.gene83217.t1	MTR_5g083790	84.000	100	16	1	100	107	206	6.62e-56	172

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene83217.t1	AT5G48760	77.000	100	23	0	1	100	107	206	7.21e-53	164
MS.gene83217.t1	AT5G48760	77.000	100	23	0	1	100	107	206	7.21e-53	164
MS.gene83217.t1	AT4G13170	75.000	100	25	0	1	100	107	206	1.18e-52	164
MS.gene83217.t1	AT3G07110	76.000	100	24	0	1	100	107	206	1.06e-51	161
MS.gene83217.t1	AT3G24830	72.000	100	28	0	1	100	107	206	1.81e-50	158
MS.gene83217.t1	AT3G07110	75.248	101	24	1	1	100	107	207	6.89e-50	157

Find 30 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AACAAAATAACTCACCTTGA+TGG	0.377070	7.3:-47321608	None:intergenic
TAGGGTGTTGAGGCTTCAAA+AGG	0.434306	7.3:+47321525	MS.gene83217:intron
AAATACTACTCAGTACTTAA+CGG	0.437826	7.3:-47321836	None:intergenic
CTCCATATGACAAAATTAAG+AGG	0.448931	7.3:+47321406	MS.gene83217:CDS
AATACTTACTTAAGAGCATC+AGG	0.452121	7.3:-47321438	None:intergenic
TGCCAGGATGAAGGTGTATG+AGG	0.452984	7.3:+47321374	None:intergenic
AATACTACTCAGTACTTAAC+GGG	0.453354	7.3:-47321835	None:intergenic
CGGGAGCAAGAATTTCAAGT+TGG	0.456903	7.3:-47321816	None:intergenic
GGGAGCAAGAATTTCAAGTT+GGG	0.464166	7.3:-47321815	None:intergenic
AGAAGCAGATCAATAAATTG+AGG	0.485847	7.3:+47321755	MS.gene83217:CDS
GGACACAAATACTGCACCCT+TGG	0.514031	7.3:+47321547	MS.gene83217:CDS
AATTATCCGCAGAGGTTGGA+TGG	0.517619	7.3:+47321572	MS.gene83217:CDS
TGGAGACAAAGAGGAAGGAA+AGG	0.523797	7.3:+47321710	MS.gene83217:CDS
GAAGCAGATCAATAAATTGA+GGG	0.524740	7.3:+47321756	MS.gene83217:CDS
GGACAATTATCCGCAGAGGT+TGG	0.557219	7.3:+47321568	MS.gene83217:CDS
ATAACTCACCTTGATGGTGC+CGG	0.561236	7.3:-47321602	None:intergenic
TGGTTGAAATTAGGGTGTTG+AGG	0.577187	7.3:+47321515	MS.gene83217:intron
GAACTACGCCGGCACCATCA+AGG	0.577844	7.3:+47321594	MS.gene83217:CDS
AACAGGAGCTGGAGACAAAG+AGG	0.580730	7.3:+47321701	MS.gene83217:intron
ATATGACAAAATTAAGAGGA+TGG	0.585968	7.3:+47321410	MS.gene83217:CDS
GCCAGGATGAAGGTGTATGA+GGG	0.597406	7.3:+47321375	None:intergenic
GGAGACAAAGAGGAAGGAAA+GGG	0.597942	7.3:+47321711	MS.gene83217:CDS
CCTCTGCGGATAATTGTCCA+AGG	0.614344	7.3:-47321564	None:intergenic
AGGGTGTTGAGGCTTCAAAA+GGG	0.617854	7.3:+47321526	MS.gene83217:intron
GGAGCTGGAGACAAAGAGGA+AGG	0.618380	7.3:+47321705	MS.gene83217:intron
GGAGCAAGAATTTCAAGTTG+GGG	0.639957	7.3:-47321814	None:intergenic
CTCTGCGGATAATTGTCCAA+GGG	0.646384	7.3:-47321563	None:intergenic
GAGGTTGGATGGAACTACGC+CGG	0.675697	7.3:+47321583	MS.gene83217:CDS
CCTTGGACAATTATCCGCAG+AGG	0.677671	7.3:+47321564	MS.gene83217:CDS
GTAGTTCCATCCAACCTCTG+CGG	0.753253	7.3:-47321578	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTGTATTTGATAAATATCTG+CGG	+	chr7.3:47321651-47321670	MS.gene83217:intron	20.0%
!!!	TTAATTTTTCACTTGTTAAC+AGG	+	chr7.3:47321684-47321703	MS.gene83217:intron	20.0%
!	ATATGACAAAATTAAGAGGA+TGG	+	chr7.3:47321410-47321429	MS.gene83217:CDS	25.0%
!!	ATCCTCTTAATTTTGTCATA+TGG	-	chr7.3:47321411-47321430	None:intergenic	25.0%
	AACAAAATAACTCACCTTGA+TGG	-	chr7.3:47321611-47321630	None:intergenic	30.0%
	AGAAGCAGATCAATAAATTG+AGG	+	chr7.3:47321755-47321774	MS.gene83217:CDS	30.0%
	CTCCATATGACAAAATTAAG+AGG	+	chr7.3:47321406-47321425	MS.gene83217:CDS	30.0%
	GAAGCAGATCAATAAATTGA+GGG	+	chr7.3:47321756-47321775	MS.gene83217:CDS	30.0%
	GAATTTGAATACTGAATGAG+TGG	+	chr7.3:47321495-47321514	MS.gene83217:intron	30.0%
	TGAATGAGTGGTTGAAATTA+GGG	+	chr7.3:47321507-47321526	MS.gene83217:intron	30.0%
!	CTCTTAATTTTGTCATATGG+AGG	-	chr7.3:47321408-47321427	None:intergenic	30.0%
!	TTAATTTTGTCATATGGAGG+TGG	-	chr7.3:47321405-47321424	None:intergenic	30.0%
!!	AATACTTACTTAAGAGCATC+AGG	-	chr7.3:47321441-47321460	None:intergenic	30.0%
	CTGAATGAGTGGTTGAAATT+AGG	+	chr7.3:47321506-47321525	MS.gene83217:intron	35.0%
!	TTTCACTTGTTAACAGGAGC+TGG	+	chr7.3:47321690-47321709	MS.gene83217:intron	40.0%
!!	GGAGCAAGAATTTCAAGTTG+GGG	-	chr7.3:47321817-47321836	None:intergenic	40.0%
!!	GGGAGCAAGAATTTCAAGTT+GGG	-	chr7.3:47321818-47321837	None:intergenic	40.0%
!!	TGGTTGAAATTAGGGTGTTG+AGG	+	chr7.3:47321515-47321534	MS.gene83217:intron	40.0%
	AATTATCCGCAGAGGTTGGA+TGG	+	chr7.3:47321572-47321591	MS.gene83217:CDS	45.0%
	CTCTGCGGATAATTGTCCAA+GGG	-	chr7.3:47321566-47321585	None:intergenic	45.0%
	GGAGACAAAGAGGAAGGAAA+GGG	+	chr7.3:47321711-47321730	MS.gene83217:CDS	45.0%
	TGGAGACAAAGAGGAAGGAA+AGG	+	chr7.3:47321710-47321729	MS.gene83217:CDS	45.0%
!	CGGGAGCAAGAATTTCAAGT+TGG	-	chr7.3:47321819-47321838	None:intergenic	45.0%
!!	AGGGTGTTGAGGCTTCAAAA+GGG	+	chr7.3:47321526-47321545	MS.gene83217:intron	45.0%
!!	ATAACTCACCTTGATGGTGC+CGG	-	chr7.3:47321605-47321624	None:intergenic	45.0%
!!	TAGGGTGTTGAGGCTTCAAA+AGG	+	chr7.3:47321525-47321544	MS.gene83217:intron	45.0%
	AACAGGAGCTGGAGACAAAG+AGG	+	chr7.3:47321701-47321720	MS.gene83217:intron	50.0%
	CCTCTGCGGATAATTGTCCA+AGG	-	chr7.3:47321567-47321586	None:intergenic	50.0%
	CCTTGGACAATTATCCGCAG+AGG	+	chr7.3:47321564-47321583	MS.gene83217:CDS	50.0%
	GGACAATTATCCGCAGAGGT+TGG	+	chr7.3:47321568-47321587	MS.gene83217:CDS	50.0%
	GGACACAAATACTGCACCCT+TGG	+	chr7.3:47321547-47321566	MS.gene83217:CDS	50.0%
	GTAGTTCCATCCAACCTCTG+CGG	-	chr7.3:47321581-47321600	None:intergenic	50.0%
	GAGGTTGGATGGAACTACGC+CGG	+	chr7.3:47321583-47321602	MS.gene83217:CDS	55.0%
	GGAGCTGGAGACAAAGAGGA+AGG	+	chr7.3:47321705-47321724	MS.gene83217:intron	55.0%
	GAACTACGCCGGCACCATCA+AGG	+	chr7.3:47321594-47321613	MS.gene83217:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.3	gene	47321381	47321849	47321381	ID=MS.gene83217
chr7.3	mRNA	47321381	47321849	47321381	ID=MS.gene83217.t1;Parent=MS.gene83217
chr7.3	exon	47321381	47321451	47321381	ID=MS.gene83217.t1.exon1;Parent=MS.gene83217.t1
chr7.3	CDS	47321381	47321451	47321381	ID=cds.MS.gene83217.t1;Parent=MS.gene83217.t1
chr7.3	exon	47321528	47321615	47321528	ID=MS.gene83217.t1.exon2;Parent=MS.gene83217.t1
chr7.3	CDS	47321528	47321615	47321528	ID=cds.MS.gene83217.t1;Parent=MS.gene83217.t1
chr7.3	exon	47321706	47321849	47321706	ID=MS.gene83217.t1.exon3;Parent=MS.gene83217.t1
chr7.3	CDS	47321706	47321849	47321706	ID=cds.MS.gene83217.t1;Parent=MS.gene83217.t1

Gene Sequence

>MS.gene83217

ATGAAGGTGTATGAGGGTATTCCACCTCCATATGACAAAATTAAGAGGATGGTTGTTCCTGATGCTCTTAAGTAAGTATTATTTCGACGATGAATTAGAATTTTTATTGAATTTGAATTTGAATACTGAATGAGTGGTTGAAATTAGGGTGTTGAGGCTTCAAAAGGGACACAAATACTGCACCCTTGGACAATTATCCGCAGAGGTTGGATGGAACTACGCCGGCACCATCAAGGTGAGTTATTTTGTTTATGTTTGAAGTTTTCTTCTTTGTATTTGATAAATATCTGCGGTTCTTAAGTTTTAATTTTTCACTTGTTAACAGGAGCTGGAGACAAAGAGGAAGGAAAGGGCTTTTGCTGCTTATGAGAAAAAGAAGCAGATCAATAAATTGAGGGCTAAAGCAGAGAAGATCGTAGACGAGAAGCTTGCTCCCCAACTTGAAATTCTTGCTCCCGTTAAGTACTGA

Protein sequence

>MS.gene83217.t1

MKVYEGIPPPYDKIKRMVVPDALKVLRLQKGHKYCTLGQLSAEVGWNYAGTIKELETKRKERAFAAYEKKKQINKLRAKAEKIVDEKLAPQLEILAPVKY