Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93887.t1 | RHN59754.1 | 91.8 | 146 | 12 | 0 | 2 | 147 | 554 | 699 | 1.50E-70 | 275.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93887.t1 | Q9C7T7 | 36.4 | 129 | 76 | 2 | 24 | 146 | 576 | 704 | 1.8e-14 | 80.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93887.t1 | A0A396I687 | 91.8 | 146 | 12 | 0 | 2 | 147 | 554 | 699 | 1.1e-70 | 275.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049150 | MS.gene93887 | 0.849003 | 4.27E-60 | -1.69E-46 |
| MS.gene049151 | MS.gene93887 | 0.851553 | 8.24E-61 | -1.69E-46 |
| MS.gene049153 | MS.gene93887 | 0.849979 | 2.28E-60 | -1.69E-46 |
| MS.gene049159 | MS.gene93887 | 0.819161 | 1.34E-52 | -1.69E-46 |
| MS.gene049233 | MS.gene93887 | 0.801688 | 8.00E-49 | -1.69E-46 |
| MS.gene049260 | MS.gene93887 | 0.815826 | 7.57E-52 | -1.69E-46 |
| MS.gene049378 | MS.gene93887 | 0.807887 | 4.06E-50 | -1.69E-46 |
| MS.gene049382 | MS.gene93887 | 0.814558 | 1.45E-51 | -1.69E-46 |
| MS.gene049435 | MS.gene93887 | 0.802972 | 4.35E-49 | -1.69E-46 |
| MS.gene049443 | MS.gene93887 | 0.801507 | 8.72E-49 | -1.69E-46 |
| MS.gene049758 | MS.gene93887 | 0.815282 | 1.00E-51 | -1.69E-46 |
| MS.gene050226 | MS.gene93887 | 0.859284 | 4.60E-63 | -1.69E-46 |
| MS.gene050227 | MS.gene93887 | 0.837737 | 4.36E-57 | -1.69E-46 |
| MS.gene050228 | MS.gene93887 | 0.846506 | 2.08E-59 | -1.69E-46 |
| MS.gene050442 | MS.gene93887 | 0.813022 | 3.16E-51 | -1.69E-46 |
| MS.gene050623 | MS.gene93887 | -0.829499 | 4.97E-55 | -1.69E-46 |
| MS.gene050642 | MS.gene93887 | 0.811504 | 6.77E-51 | -1.69E-46 |
| MS.gene050680 | MS.gene93887 | 0.835705 | 1.44E-56 | -1.69E-46 |
| MS.gene050762 | MS.gene93887 | 0.847292 | 1.27E-59 | -1.69E-46 |
| MS.gene050763 | MS.gene93887 | 0.849586 | 2.94E-60 | -1.69E-46 |
| MS.gene050826 | MS.gene93887 | 0.803191 | 3.92E-49 | -1.69E-46 |
| MS.gene050987 | MS.gene93887 | 0.808088 | 3.68E-50 | -1.69E-46 |
| MS.gene051535 | MS.gene93887 | 0.812884 | 3.39E-51 | -1.69E-46 |
| MS.gene051860 | MS.gene93887 | 0.825482 | 4.58E-54 | -1.69E-46 |
| MS.gene051983 | MS.gene93887 | 0.819053 | 1.42E-52 | -1.69E-46 |
| MS.gene052255 | MS.gene93887 | 0.80019 | 1.62E-48 | -1.69E-46 |
| MS.gene052426 | MS.gene93887 | 0.835555 | 1.57E-56 | -1.69E-46 |
| MS.gene053074 | MS.gene93887 | 0.811123 | 8.19E-51 | -1.69E-46 |
| MS.gene053184 | MS.gene93887 | 0.810522 | 1.11E-50 | -1.69E-46 |
| MS.gene053216 | MS.gene93887 | 0.832931 | 7.13E-56 | -1.69E-46 |
| MS.gene053494 | MS.gene93887 | 0.830534 | 2.78E-55 | -1.69E-46 |
| MS.gene053559 | MS.gene93887 | 0.80523 | 1.48E-49 | -1.69E-46 |
| MS.gene053577 | MS.gene93887 | 0.83167 | 1.46E-55 | -1.69E-46 |
| MS.gene053836 | MS.gene93887 | 0.828509 | 8.64E-55 | -1.69E-46 |
| MS.gene054184 | MS.gene93887 | 0.815896 | 7.31E-52 | -1.69E-46 |
| MS.gene054185 | MS.gene93887 | 0.817202 | 3.73E-52 | -1.69E-46 |
| MS.gene054379 | MS.gene93887 | 0.863063 | 3.25E-64 | -1.69E-46 |
| MS.gene054542 | MS.gene93887 | 0.820712 | 5.94E-53 | -1.69E-46 |
| MS.gene054930 | MS.gene93887 | 0.858392 | 8.50E-63 | -1.69E-46 |
| MS.gene05510 | MS.gene93887 | 0.851731 | 7.33E-61 | -1.69E-46 |
| MS.gene055356 | MS.gene93887 | 0.804258 | 2.36E-49 | -1.69E-46 |
| MS.gene055358 | MS.gene93887 | 0.813385 | 2.63E-51 | -1.69E-46 |
| MS.gene055701 | MS.gene93887 | 0.837597 | 4.73E-57 | -1.69E-46 |
| MS.gene056069 | MS.gene93887 | -0.803215 | 3.88E-49 | -1.69E-46 |
| MS.gene056760 | MS.gene93887 | 0.803073 | 4.15E-49 | -1.69E-46 |
| MS.gene056875 | MS.gene93887 | 0.802303 | 5.98E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene93887 | 0.817626 | 2.99E-52 | -1.69E-46 |
| MS.gene057394 | MS.gene93887 | 0.823697 | 1.20E-53 | -1.69E-46 |
| MS.gene057469 | MS.gene93887 | 0.828823 | 7.25E-55 | -1.69E-46 |
| MS.gene05761 | MS.gene93887 | 0.802625 | 5.13E-49 | -1.69E-46 |
| MS.gene057750 | MS.gene93887 | 0.84457 | 6.96E-59 | -1.69E-46 |
| MS.gene057754 | MS.gene93887 | 0.835579 | 1.55E-56 | -1.69E-46 |
| MS.gene057869 | MS.gene93887 | 0.817214 | 3.70E-52 | -1.69E-46 |
| MS.gene058146 | MS.gene93887 | 0.813787 | 2.14E-51 | -1.69E-46 |
| MS.gene058399 | MS.gene93887 | 0.815579 | 8.60E-52 | -1.69E-46 |
| MS.gene058595 | MS.gene93887 | 0.820097 | 8.21E-53 | -1.69E-46 |
| MS.gene058749 | MS.gene93887 | 0.805027 | 1.63E-49 | -1.69E-46 |
| MS.gene058833 | MS.gene93887 | 0.814806 | 1.28E-51 | -1.69E-46 |
| MS.gene058949 | MS.gene93887 | 0.800349 | 1.50E-48 | -1.69E-46 |
| MS.gene059124 | MS.gene93887 | 0.814278 | 1.67E-51 | -1.69E-46 |
| MS.gene059128 | MS.gene93887 | 0.863194 | 2.96E-64 | -1.69E-46 |
| MS.gene059161 | MS.gene93887 | 0.833855 | 4.20E-56 | -1.69E-46 |
| MS.gene059285 | MS.gene93887 | 0.803387 | 3.57E-49 | -1.69E-46 |
| MS.gene05984 | MS.gene93887 | 0.809535 | 1.80E-50 | -1.69E-46 |
| MS.gene059899 | MS.gene93887 | 0.804705 | 1.90E-49 | -1.69E-46 |
| MS.gene060084 | MS.gene93887 | 0.827027 | 1.96E-54 | -1.69E-46 |
| MS.gene060322 | MS.gene93887 | 0.803442 | 3.48E-49 | -1.69E-46 |
| MS.gene060480 | MS.gene93887 | 0.812191 | 4.80E-51 | -1.69E-46 |
| MS.gene060495 | MS.gene93887 | 0.824374 | 8.35E-54 | -1.69E-46 |
| MS.gene060683 | MS.gene93887 | 0.829939 | 3.89E-55 | -1.69E-46 |
| MS.gene060685 | MS.gene93887 | 0.82004 | 8.46E-53 | -1.69E-46 |
| MS.gene060802 | MS.gene93887 | 0.841519 | 4.52E-58 | -1.69E-46 |
| MS.gene060803 | MS.gene93887 | 0.825646 | 4.18E-54 | -1.69E-46 |
| MS.gene060998 | MS.gene93887 | 0.836096 | 1.14E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93887.t1 | MTR_4g035200 | 82.143 | 112 | 20 | 0 | 1 | 112 | 337 | 448 | 7.97e-61 | 194 |
| MS.gene93887.t1 | MTR_4g035200 | 82.143 | 112 | 20 | 0 | 1 | 112 | 530 | 641 | 2.54e-60 | 197 |
| MS.gene93887.t1 | MTR_3g110860 | 31.373 | 153 | 88 | 5 | 1 | 144 | 544 | 688 | 6.10e-12 | 62.4 |
| MS.gene93887.t1 | MTR_3g110860 | 31.373 | 153 | 88 | 5 | 1 | 144 | 544 | 688 | 7.71e-12 | 62.4 |
| MS.gene93887.t1 | MTR_5g090100 | 32.394 | 142 | 85 | 6 | 3 | 140 | 562 | 696 | 3.33e-11 | 60.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene93887.t1 | AT1G72180 | 38.158 | 152 | 88 | 2 | 1 | 146 | 553 | 704 | 9.02e-23 | 93.6 |
| MS.gene93887.t1 | AT5G49660 | 33.784 | 148 | 87 | 6 | 4 | 145 | 534 | 676 | 1.18e-15 | 73.2 |
| MS.gene93887.t1 | AT5G25930 | 30.201 | 149 | 84 | 5 | 1 | 142 | 567 | 702 | 1.48e-13 | 67.4 |
| MS.gene93887.t1 | AT4G28490 | 31.757 | 148 | 84 | 6 | 1 | 145 | 570 | 703 | 1.35e-11 | 61.6 |
Find 20 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTCTGGTGGAATTCCTTT+TGG | 0.134344 | 4.2:-70407464 | MS.gene93887:CDS |
| TTCGATTCTTATTGCATCTA+TGG | 0.229360 | 4.2:+70407249 | None:intergenic |
| CTTGACTCGATAAACCTTTC+CGG | 0.295561 | 4.2:+70407094 | None:intergenic |
| TTGGATGAAGATAATTTAAT+CGG | 0.349048 | 4.2:-70407128 | MS.gene93887:CDS |
| TGCAGATGAAGTACGTCATT+TGG | 0.396558 | 4.2:-70407147 | MS.gene93887:CDS |
| AAGATTTGTGATAAAGATCA+TGG | 0.425316 | 4.2:-70407350 | MS.gene93887:CDS |
| TCTGAGAATTCTCTTTCTGG+TGG | 0.488085 | 4.2:-70407476 | MS.gene93887:CDS |
| CCAATAATCAAAATCCCAAA+AGG | 0.502715 | 4.2:+70407449 | None:intergenic |
| TTAATCGGCTACGGTGGCAC+CGG | 0.506802 | 4.2:-70407113 | MS.gene93887:CDS |
| TTTGATTATTGGTGGAGAGA+AGG | 0.520661 | 4.2:-70407438 | MS.gene93887:CDS |
| GAAGATAATTTAATCGGCTA+CGG | 0.544968 | 4.2:-70407122 | MS.gene93887:CDS |
| CGGCTACGGTGGCACCGGAA+AGG | 0.572431 | 4.2:-70407108 | MS.gene93887:CDS |
| GAAAATGAAACTAAGTTCAG+TGG | 0.577728 | 4.2:-70407504 | None:intergenic |
| CATCTGCATCGATATCCACT+TGG | 0.579727 | 4.2:+70407162 | None:intergenic |
| AAAGATCATGGTCATAGAAG+AGG | 0.596154 | 4.2:-70407338 | MS.gene93887:CDS |
| AAGATCATGGTCATAGAAGA+GGG | 0.602774 | 4.2:-70407337 | MS.gene93887:CDS |
| GCATCTATGGATCACAATCA+CGG | 0.633777 | 4.2:+70407262 | None:intergenic |
| GATAATTTAATCGGCTACGG+TGG | 0.640512 | 4.2:-70407119 | MS.gene93887:CDS |
| AGTTGAAGAAAACCGCATAG+TGG | 0.665444 | 4.2:-70407073 | None:intergenic |
| GCTTTACGGCCACCACTATG+CGG | 0.684678 | 4.2:+70407061 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAGAAAAAAATTTACAAAA+AGG | - | chr4.2:70407349-70407368 | MS.gene93887:CDS | 10.0% |
| !! | TTGGATGAAGATAATTTAAT+CGG | - | chr4.2:70407448-70407467 | MS.gene93887:CDS | 20.0% |
| ! | AAATTTACAAAAAGGCGAAA+TGG | - | chr4.2:70407357-70407376 | MS.gene93887:CDS | 25.0% |
| ! | AAGATTTGTGATAAAGATCA+TGG | - | chr4.2:70407226-70407245 | MS.gene93887:CDS | 25.0% |
| AAATGGAAGCAAGTCAAAAA+TGG | - | chr4.2:70407374-70407393 | MS.gene93887:CDS | 30.0% | |
| CCAATAATCAAAATCCCAAA+AGG | + | chr4.2:70407130-70407149 | None:intergenic | 30.0% | |
| GAAGATAATTTAATCGGCTA+CGG | - | chr4.2:70407454-70407473 | MS.gene93887:CDS | 30.0% | |
| TTCGATTCTTATTGCATCTA+TGG | + | chr4.2:70407330-70407349 | None:intergenic | 30.0% | |
| !! | TTTTCTGAGAATTCTCTTTC+TGG | - | chr4.2:70407097-70407116 | MS.gene93887:CDS | 30.0% |
| !!! | CCTTTTGGGATTTTGATTAT+TGG | - | chr4.2:70407127-70407146 | MS.gene93887:CDS | 30.0% |
| !!! | TTTGGGATTTTGATTATTGG+TGG | - | chr4.2:70407130-70407149 | MS.gene93887:CDS | 30.0% |
| AAAGATCATGGTCATAGAAG+AGG | - | chr4.2:70407238-70407257 | MS.gene93887:CDS | 35.0% | |
| AAGATCATGGTCATAGAAGA+GGG | - | chr4.2:70407239-70407258 | MS.gene93887:CDS | 35.0% | |
| TTTGATTATTGGTGGAGAGA+AGG | - | chr4.2:70407138-70407157 | MS.gene93887:CDS | 35.0% | |
| ! | GCAGTTTTCATTGAAGTTTG+TGG | + | chr4.2:70407202-70407221 | None:intergenic | 35.0% |
| !! | TTTCTGGTGGAATTCCTTTT+GGG | - | chr4.2:70407113-70407132 | MS.gene93887:CDS | 35.0% |
| GATAATTTAATCGGCTACGG+TGG | - | chr4.2:70407457-70407476 | MS.gene93887:CDS | 40.0% | |
| GCATCTATGGATCACAATCA+CGG | + | chr4.2:70407317-70407336 | None:intergenic | 40.0% | |
| TCTGAGAATTCTCTTTCTGG+TGG | - | chr4.2:70407100-70407119 | MS.gene93887:CDS | 40.0% | |
| TGCAGATGAAGTACGTCATT+TGG | - | chr4.2:70407429-70407448 | MS.gene93887:CDS | 40.0% | |
| ! | ACAAGCATCTTTTCACCAAG+TGG | - | chr4.2:70407399-70407418 | MS.gene93887:CDS | 40.0% |
| ! | CTTGACTCGATAAACCTTTC+CGG | + | chr4.2:70407485-70407504 | None:intergenic | 40.0% |
| !! | CTTTCTGGTGGAATTCCTTT+TGG | - | chr4.2:70407112-70407131 | MS.gene93887:CDS | 40.0% |
| ! | CATCTGCATCGATATCCACT+TGG | + | chr4.2:70407417-70407436 | None:intergenic | 45.0% |
| ! | GGTGGAGAGAAGGCTTTTGT+TGG | - | chr4.2:70407148-70407167 | MS.gene93887:CDS | 50.0% |
| TTAATCGGCTACGGTGGCAC+CGG | - | chr4.2:70407463-70407482 | MS.gene93887:CDS | 55.0% | |
| ! | CGGCTACGGTGGCACCGGAA+AGG | - | chr4.2:70407468-70407487 | MS.gene93887:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 70407076 | 70407522 | 70407076 | ID=MS.gene93887 |
| chr4.2 | mRNA | 70407076 | 70407522 | 70407076 | ID=MS.gene93887.t1;Parent=MS.gene93887 |
| chr4.2 | exon | 70407076 | 70407522 | 70407076 | ID=MS.gene93887.t1.exon1;Parent=MS.gene93887.t1 |
| chr4.2 | CDS | 70407076 | 70407522 | 70407076 | ID=cds.MS.gene93887.t1;Parent=MS.gene93887.t1 |
| Gene Sequence |
| Protein sequence |
